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Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F73 I2 R1	76	833.2	37290668	99.4%	37066923	136.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	AE015451_phaM1	1,738,086	Δ40,059 bp		[PP_1532 CDS (putative Phage integrase)]–[PP_1586 CDS (putative Killer protein)]	60 genes [PP_1532 CDS (putative Phage integrase)], PP_1533 CDS (putative Excisionase), PP_1534 CDS (conserved protein of unknown function), PP_1535 CDS (putative Methyltransferase), PP_1536 CDS (conserved protein of unknown function), PP_1537 CDS (conserved protein of unknown function), PP_5472 CDS (conserved exported protein of unknown function), PP_1538 CDS (conserved protein of unknown function), PP_1539 CDS (conserved protein of unknown function), PP_1540 CDS (conserved protein of unknown function), PP_1541 CDS (putative Methyltransferase), PP_1542 CDS (conserved protein of unknown function), PP_1543 CDS (conserved protein of unknown function), PP_1544 CDS (conserved protein of unknown function), PP_1545 CDS (conserved protein of unknown function), PP_1546 CDS (conserved protein of unknown function), PP_5473 CDS (putative transcriptional regulator_ LuxR family), PP_5474 CDS (conserved protein of unknown function of phage origin), PP_1548 CDS (conserved protein of unknown function), PP_5744 CDS (conserved protein of unknown function_ phage origin), PP_1549 CDS (Transcriptional regulator_ Cro_CI family), PP_1550 CDS (Transcriptional regulator_ Cro_CI family), PP_1551 CDS (putative Phage replication protein O), PP_1552 CDS (putative Phage replication protein), PP_1553 CDS (conserved protein of unknown function), PP_1554 CDS (conserved protein of unknown function), PP_1555 CDS (putative Phage integrase), PP_1556 CDS (conserved protein of unknown function), PP_1557 CDS (conserved protein of unknown function), PP_1558 CDS (conserved protein of unknown function), PP_1559 CDS (Holin), PP_1560 CDS (conserved protein of unknown function), PP_1561 CDS (putative Phage holin), PP_1562 CDS (putative Phage terminase_ small subunit), PP_1563 CDS (putative Phage terminase_ large subunit), PP_1564 CDS (conserved exported protein of unknown function), PP_1565 CDS (Phage portal protein_ HK97 family), PP_1566 CDS (Peptidase U35), PP_1567 CDS (Capsid protein), PP_1568 CDS (conserved protein of unknown function), PP_1569 CDS (conserved protein of unknown function), PP_1570 CDS (Head‑tail adaptor), PP_1571 CDS (conserved protein of unknown function), PP_1572 CDS (conserved protein of unknown function), PP_1573 CDS (putative Major tail protein), PP_1574 CDS (conserved protein of unknown function), PP_1575 CDS (conserved protein of unknown function), PP_1576 CDS (putative Immunity protein), PP_1577 CDS (Phage tail tape meausure protein_ lambda family), PP_1578 CDS (conserved protein of unknown function), PP_5475 CDS (conserved protein of unknown function), PP_1579 CDS (conserved protein of unknown function), PP_1580 CDS (conserved protein of unknown function), PP_1581 CDS (BNR domain protein), PP_1582 CDS (conserved protein of unknown function), PP_1583 CDS (putative Structural protein P5), PP_1584 CDS (conserved protein of unknown function), PP_5476 CDS (conserved protein of unknown function), PP_1585 CDS (putative Antidote protein), [PP_1586 CDS (putative Killer protein)]

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	AE015451_phaM1	1738086	1778144	40059	909 [0]	[0] 907	[PP_1532 CDS (putative Phage integrase)]–[PP_1586 CDS (putative Killer protein)]	[PP_1532 CDS (putative Phage integrase)],PP_1533 CDS (putative Excisionase),PP_1534 CDS (conserved protein of unknown function),PP_1535 CDS (putative Methyltransferase),PP_1536 CDS (conserved protein of unknown function),PP_1537 CDS (conserved protein of unknown function),PP_5472 CDS (conserved exported protein of unknown function),PP_1538 CDS (conserved protein of unknown function),PP_1539 CDS (conserved protein of unknown function),PP_1540 CDS (conserved protein of unknown function),PP_1541 CDS (putative Methyltransferase),PP_1542 CDS (conserved protein of unknown function),PP_1543 CDS (conserved protein of unknown function),PP_1544 CDS (conserved protein of unknown function),PP_1545 CDS (conserved protein of unknown function),PP_1546 CDS (conserved protein of unknown function),PP_5473 CDS (putative transcriptional regulator_ LuxR family),PP_5474 CDS (conserved protein of unknown function of phage origin),PP_1548 CDS (conserved protein of unknown function),PP_5744 CDS (conserved protein of unknown function_ phage origin),PP_1549 CDS (Transcriptional regulator_ Cro_CI family),PP_1550 CDS (Transcriptional regulator_ Cro_CI family),PP_1551 CDS (putative Phage replication protein O),PP_1552 CDS (putative Phage replication protein),PP_1553 CDS (conserved protein of unknown function),PP_1554 CDS (conserved protein of unknown function),PP_1555 CDS (putative Phage integrase),PP_1556 CDS (conserved protein of unknown function),PP_1557 CDS (conserved protein of unknown function),PP_1558 CDS (conserved protein of unknown function),PP_1559 CDS (Holin),PP_1560 CDS (conserved protein of unknown function),PP_1561 CDS (putative Phage holin),PP_1562 CDS (putative Phage terminase_ small subunit),PP_1563 CDS (putative Phage terminase_ large subunit),PP_1564 CDS (conserved exported protein of unknown function),PP_1565 CDS (Phage portal protein_ HK97 family),PP_1566 CDS (Peptidase U35),PP_1567 CDS (Capsid protein),PP_1568 CDS (conserved protein of unknown function),PP_1569 CDS (conserved protein of unknown function),PP_1570 CDS (Head‑tail adaptor),PP_1571 CDS (conserved protein of unknown function),PP_1572 CDS (conserved protein of unknown function),PP_1573 CDS (putative Major tail protein),PP_1574 CDS (conserved protein of unknown function),PP_1575 CDS (conserved protein of unknown function),PP_1576 CDS (putative Immunity protein),PP_1577 CDS (Phage tail tape meausure protein_ lambda family),PP_1578 CDS (conserved protein of unknown function),PP_5475 CDS (conserved protein of unknown function),PP_1579 CDS (conserved protein of unknown function),PP_1580 CDS (conserved protein of unknown function),PP_1581 CDS (BNR domain protein),PP_1582 CDS (conserved protein of unknown function),PP_1583 CDS (putative Structural protein P5),PP_1584 CDS (conserved protein of unknown function),PP_5476 CDS (conserved protein of unknown function),PP_1585 CDS (putative Antidote protein),[PP_1586 CDS (putative Killer protein)]

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451_phaM1	= 1738085	0 (0.000)	907 (1.090)	125/270	0.2	100%	intergenic (‑67/+1)	yffB CDS (putative glutathione‑dependent thiol reductase)/PP_1532 CDS (putative Phage integrase)	–/–
*	?	AE015451_phaM1	1778145 =	0 (0.000)	907 (1.090)	125/270	0.2	100%	intergenic (‑1/‑196)	PP_1586 CDS (putative Killer protein)/PP_1587 CDS (NhaP‑type Na+_H+ and K+_H+ antiporters)	–/–

GATK/CNVnator alignment

N/A