Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F84 I10 R1 267 426.1 13521957 97.0% 13116298 150.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,401,607 C→T T312T (ACC→ACT)  hflX → GTPase, stimulated by 50S subunit binding; Mn(2+) homeostasis regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,401,6070CT97.8% 1551.8 / 12.7 449T312T (ACC→ACT) hflXGTPase, stimulated by 50S subunit binding; Mn(2+) homeostasis regulator
Reads supporting (aligned to +/- strand):  ref base C (4/6);  new base T (205/234);  total (209/240)
Fisher's exact test for biased strand distribution p-value = 7.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.12e-01

Only 100 of 455 total aligned reads displayed.

TGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCTTTTTCAGGCTTTGACGGAGCGGCTT  >  NC_000913/4401459‑4401754
                                                                                                                                                    |                                                                                                                                                   
tggtggCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAActct                                                                                                                                                   >  8:111820/1‑151 (MQ=255)
tggtggCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAActct                                                                                                                                                   >  4:518122/1‑151 (MQ=255)
    ggcggcATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTctgctg                                                                                                                                               <  1:1155302/151‑1 (MQ=255)
        gcATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGtga                                                                                                                                           <  4:1320511/151‑1 (MQ=255)
         cATTTAAAGCCACGTTACAAGAGCCGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAACAAAACCTCGAAGCGGTGAATACGGTTCTTGAAGAGCTCGACGCTCACGAGATCCGCACTCTGCTGGtgat                                                                                                                                          >  2:1159227/1‑151 (MQ=255)
         cATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGtgat                                                                                                                                          <  3:1068278/151‑1 (MQ=255)
          aTTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGtgatg                                                                                                                                         <  5:519905/151‑1 (MQ=255)
            ttAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGtgatga                                                                                                                                        <  5:60852/150‑1 (MQ=255)
             tAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGaa                                                                                                                                       <  6:1228793/150‑1 (MQ=255)
             tAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAAc                                                                                                                                      >  7:695781/1‑151 (MQ=255)
              aaaGCCACGTTACAAGAGACGCGGCAAGCGACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGCTCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAAc                                                                                                                                      >  1:1049362/1‑150 (MQ=255)
              aaaGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAAc                                                                                                                                      <  1:625737/150‑1 (MQ=255)
               aaGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGGGATGAACa                                                                                                                                     >  2:1540470/1‑150 (MQ=255)
                  ccACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGAt                                                                                                                                 <  5:157361/151‑1 (MQ=255)
                   cACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATc                                                                                                                                <  4:393549/151‑1 (MQ=255)
                   cACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATc                                                                                                                                <  5:1383042/151‑1 (MQ=255)
                   cACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATc                                                                                                                                <  7:61595/151‑1 (MQ=255)
                      gTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGAGGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGat                                                                                                                             <  2:899634/151‑1 (MQ=255)
                         aCAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATg                                                                                                                          >  2:655495/1‑151 (MQ=255)
                          cAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGc                                                                                                                         >  6:1572332/1‑151 (MQ=255)
                           aaGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCt                                                                                                                        <  8:893489/151‑1 (MQ=255)
                              agaCGCGGCTAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCTTGTACAAGAAAACTTCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGa                                                                                                                     <  8:1634991/151‑1 (MQ=255)
                               gaCGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGaa                                                                                                                    <  2:1628536/151‑1 (MQ=255)
                               gaCGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGaa                                                                                                                    <  7:342954/151‑1 (MQ=255)
                               caCGCGGCAAGCCACATTACTGCGGCACGGCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGaa                                                                                                                    <  3:666990/150‑1 (MQ=255)
                                  gcgGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGa                                                                                                                     >  6:1708145/1‑147 (MQ=255)
                                  gcgGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGCATGTGCGTGTACAAGAAAACATCGAAGAGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTCGTGATGAAGAAGATCGATATGCTGGa                                                                                                                     >  1:100041/1‑147 (MQ=255)
                                   cgGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGAtt                                                                                                                >  4:1036276/1‑151 (MQ=255)
                                     gCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTc                                                                                                              <  5:427485/151‑1 (MQ=255)
                                     gCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTc                                                                                                              <  1:199230/151‑1 (MQ=255)
                                      cAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCg                                                                                                             <  8:1235774/151‑1 (MQ=255)
                                       aaGCCACATTACTGCTGCACGTCATTGATGCGGCGGAGGTGCGGGGACAAGAAAACATCGAAGCGGTGAATACGGTTATTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGa                                                                                                            <  8:213912/151‑1 (MQ=255)
                                          ccACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGCTCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGCTTTCGAAcc                                                                                                         >  6:861204/1‑151 (MQ=255)
                                          ccACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAAcc                                                                                                         >  6:1194204/1‑151 (MQ=255)
                                           cacaTTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACcg                                                                                                        <  3:1201908/151‑1 (MQ=255)
                                           cacaTTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCAAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACcg                                                                                                        >  1:555772/1‑151 (MQ=255)
                                            acaTTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACcgc                                                                                                       <  2:732620/151‑1 (MQ=255)
                                              aTTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGt                                                                                                     <  3:406957/151‑1 (MQ=255)
                                               ttACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGt                                                                                                     >  1:372904/1‑150 (MQ=255)
                                                tACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTAt                                                                                                   >  5:1496850/1‑151 (MQ=255)
                                                 aCTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTAtt                                                                                                  >  6:573026/1‑151 (MQ=255)
                                                    gctgcACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGa                                                                                                <  4:1418758/150‑1 (MQ=255)
                                                      tgcACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCg                                                                                             >  6:909060/1‑151 (MQ=255)
                                                       gcACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCgg                                                                                            <  3:1240970/151‑1 (MQ=255)
                                                        cACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGCTCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCggg                                                                                           >  2:180960/1‑151 (MQ=255)
                                                        cACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCggg                                                                                           <  3:54313/151‑1 (MQ=255)
                                                          cGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGAc                                                                                         <  1:1413594/151‑1 (MQ=255)
                                                            tCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACg                                                                                        >  6:1364786/1‑150 (MQ=255)
                                                            tCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACg                                                                                        >  7:1611331/1‑150 (MQ=255)
                                                             cATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGa                                                                                       <  2:386513/150‑1 (MQ=255)
                                                                tGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGCTCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAag                                                                                     >  1:825734/1‑149 (MQ=255)
                                                                tGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAaga                                                                                    <  4:118445/150‑1 (MQ=255)
                                                                    gcggcgGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACa                                                                               <  7:85929/151‑1 (MQ=255)
                                                                       gcggATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAaac                                                                            >  8:379520/1‑151 (MQ=255)
                                                                        cggATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAaacc                                                                           <  1:915060/151‑1 (MQ=255)
                                                                         ggATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAaaccg                                                                          >  1:990918/1‑151 (MQ=255)
                                                                             gtgCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAaaccgaacc                                                                      >  1:456935/1‑151 (MQ=255)
                                                                             gtgCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAaaccgaac                                                                       <  3:937654/150‑1 (MQ=255)
                                                                               gCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCgt                                                                    >  3:140903/1‑151 (MQ=255)
                                                                                 gtgtACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGACCCgtgt                                                                  >  2:132217/1‑151 (MQ=255)
                                                                                  ggtACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATGGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTc                                                                 <  4:1678929/150‑1 (MQ=255)
                                                                                        aGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCtt                                                           >  5:739612/1‑151 (MQ=255)
                                                                                         gAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCttt                                                          >  5:1195246/1‑151 (MQ=255)
                                                                                          aaaaCATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTc                                                         >  8:133269/1‑151 (MQ=255)
                                                                                           aaaCATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTcc                                                        >  5:1607650/1‑151 (MQ=255)
                                                                                            aaCATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCg                                                       <  2:534639/151‑1 (MQ=255)
                                                                                            aaCATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCg                                                       <  3:775246/151‑1 (MQ=255)
                                                                                              cATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGc                                                      >  5:1082990/1‑150 (MQ=255)
                                                                                                tCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGcac                                                    >  3:1333605/1‑150 (MQ=255)
                                                                                                tCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGcac                                                    >  3:863284/1‑150 (MQ=255)
                                                                                                 cGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAACATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGcac                                                    >  7:1000125/1‑149 (MQ=255)
                                                                                                  gAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGcac                                                    >  8:788616/1‑148 (MQ=255)
                                                                                                  gAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATAAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGcac                                                    >  8:1051939/1‑148 (MQ=255)
                                                                                                    aGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGAcc                                               >  3:170920/1‑151 (MQ=255)
                                                                                                           aaTACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCgg                                        <  5:1237596/151‑1 (MQ=255)
                                                                                                            aTACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGCCCGGAGCggg                                       >  8:1460190/1‑151 (MQ=255)
                                                                                                            aTACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCggg                                       <  2:1376215/151‑1 (MQ=255)
                                                                                                              aCGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGATCAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCgggg                                      <  1:1646520/150‑1 (MQ=255)
                                                                                                               cGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGa                                     <  1:1200540/150‑1 (MQ=255)
                                                                                                                ggTTCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATa                                   <  2:777449/151‑1 (MQ=255)
                                                                                                                   tCTTGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATAccc                                >  1:1519284/1‑150 (MQ=255)
                                                                                                                     ttGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACcac                               >  6:49037/1‑150 (MQ=255)
                                                                                                                      tGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACcaca                              <  2:257815/150‑1 (MQ=255)
                                                                                                                      tGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACcaca                              <  5:887135/150‑1 (MQ=255)
                                                                                                                      tGAAGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACca                                <  4:1630100/148‑1 (MQ=255)
                                                                                                                      tGAAGAGATCGACGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACcaca                              <  7:1342985/150‑1 (MQ=255)
                                                                                                                        aaGAGATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGCATACCACAGCt                           >  4:685746/1‑151 (MQ=255)
                                                                                                                         agagATCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATCTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCtt                          <  6:720332/151‑1 (MQ=255)
                                                                                                                          gagaTCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCttt                         <  4:919158/151‑1 (MQ=255)
                                                                                                                           agaTCGACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCtttt                        <  7:93991/151‑1 (MQ=255)
                                                                                                                                gACGCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCTTTTTCAgg                   <  5:982639/151‑1 (MQ=255)
                                                                                                                                   gCTCACGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCTTTTTCAGGCtt                >  6:691782/1‑151 (MQ=255)
                                                                                                                                        cGAGATCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCTTTTTCAGGCTTTGACg           <  5:359237/151‑1 (MQ=255)
                                                                                                                                            aTCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACGGACCGGAGCGGGGATACCACAGCTTTTTCAGGCTTTGACGGAGc       <  7:234/151‑1 (MQ=255)
                                                                                                                                            aTCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCTTTTTCAGGCTTTGACGGAGc       <  6:305182/151‑1 (MQ=255)
                                                                                                                                            aTCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCTTTTTCAGGCTTTGACGGAGc       >  1:258720/1‑151 (MQ=255)
                                                                                                                                            aTCCCAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCTTTTTCAGGCTTTGACGGAGc       <  1:768605/151‑1 (MQ=255)
                                                                                                                                              cccAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCTTTTTCAGGCTTTGACGGAGCgg     <  8:535270/151‑1 (MQ=255)
                                                                                                                                                cAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCTTTTTCAGGCTTTGACGGAGCGGCt   >  7:1133167/1‑151 (MQ=255)
                                                                                                                                                cAACTCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCTTTTTCAGGCTTTGACGGAGCGGCt   >  8:666457/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCTTTTTCAGGCTTTGACGGAGCGGCTT  >  NC_000913/4401459‑4401754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A