Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F91 I0 R2 42 81.4 1505765 88.3% 1329590 282.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,173,361 Δ2 bp 100% pseudogene (1‑2/442 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0009132,173,3610C→.100.0% 14.1 67pseudogene (2/442 nt)gatCpseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
Reads supporting (aligned to +/- strand):  new base (33/28):  ref base (2/4):  total (35/32)
Fisher's exact test strand distribution p-value = 4.14e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.52e-03
*NC_0009132,173,3620C→.100.0% 12.4 67pseudogene (1/442 nt)gatCpseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
Reads supporting (aligned to +/- strand):  new base (32/28):  ref base (3/3):  total (35/32)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.29e-04

GGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAA  >  NC_000913/2173062‑2173654
                                                                                                                                                                                                                                                                                                           ||                                                                                                                                                                                                                                                                                                    
ggTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGCGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACCAACCGCAATTCAAATGGTGCGGGGGATAAAAATCAGGCTTGCCGATaacac                                                                                                                                                                                                                                                                                                       >  2:62799/1‑300 (MQ=255)
  tAATGGGAGACCCGCCCTGAGCCATGGAAGCAACCATACCCCCGGCTTTTACCGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGAGCCACAGGGTGATGCTCATAATGATGACACCCGAGATT‑AGGTGCGGAACAGATGTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATaccaccgc                                                                                                                                                                                                                                                                                                    <  1:220799/300‑3 (MQ=255)
    ttGGGAGACCCCCCCTGATCCATTGAAGCCACCCGACCCCCTGCTGTTAACGCCCCAGCATTAGCCGCCAGGTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGGGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATaccaccgccg                                                                                                                                                                                                                                                                                                  <  2:130411/300‑5 (MQ=255)
    aTGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCACACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCAACGAAGAAGCCGATGGTGGCAAGATCGCCCAACGGAAGCACCTGATTACCCGGCACACAAACAGCAAGTAAAATGGTGAGTGGGAGAAAAATCAGGCTTGCCGATACCAcacccg                                                                                                                                                                                                                                                                                                  >  1:130416/1‑299 (MQ=255)
     tGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACCGGGTGATGCTCATCATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCCAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATaccaccgccgt                                                                                                                                                                                                                                                                                                 >  1:221071/1‑296 (MQ=255)
       ggAAAACCGCCCTGAGCCATGGAAGCCACCCTACCACCGGCTGTTAACTCCCCATCATTAGCCGCTAGTGGGGTGTGAAAACCAATAGTTTGCGTCGCGATCCACAGGGGGATGCTCATAATGACGACACCCGAGACTAAGGGGCGGAACAGATTTCCACGAGGCACGGCGACCGCCAGCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATaccaccgccgta                                                                                                                                                                                                                                                                                                <  1:404618/300‑7 (MQ=255)
          aaaCCGCCCTGAGCCAATGAAGCCACCATACCCCCGGCTTGTAACGCCCCAGCAGTAGCCGCCAGGGGGGTTGGCAAACCAATAGTTTGGGTCGCGATCCAAAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAGATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTc                                                                                                                                                                                                                                                                                          <  2:195096/300‑1 (MQ=255)
            aCCGCCCTGATCCATTGAAGCCACCATACCACCGGCGTTTAACGCGCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTATGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTccc                                                                                                                                                                                                                                                                                        <  1:394222/301‑1 (MQ=255)
                   agaaagaTTGAAGACACCATACCCCCGGCTTTTAACGCCCCAGCATGAGCCGCCAGTTGGGTGTGCAAACCAAGAGTTTGCGTCGCGAGCCACAGGGTGATGCTCATAATGATGACACCGGAGATTAAGGGGAGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTTGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCagcagc                                                                                                                                                                                                                                                                                  <  1:617039/294‑1 (MQ=255)
                       ccATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAACCGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCC‑ACAAC‑CCCCCCCGTATGCCCCGGAAGCAACga                                                                                                                                                                                                                                                                             >  2:188532/1‑300 (MQ=255)
                         aTTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATTGTGGCACGATCGCCAAACGGCAGCCCCTGCTTCCCCGGCCCACACACCGCAATTAAAATTGTAAGTGGGATAAAAATCAGGATTGCCGATaacaccaccctatggcccaccagcctgtcc                                                                                                                                                                                                                                                                              >  2:617015/1‑276 (MQ=255)
                         aTTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGAGGGTGGCAAGATCGCCAAAAGGCAGCACCTGATTACCCGGCCAACAAACAGCAAATAAAATGGGGAGTGGGATAAAAATCGGGCTTGCCGATA‑CA‑CCCGCCGAAGGTCCCAGAAGAAAAGcgg                                                                                                                                                                                                                                                                           >  2:342148/1‑299 (MQ=255)
                                  accaTACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGAGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATGAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGc                                                                                                                                                                                                                                                                  <  1:227997/301‑1 (MQ=255)
                                                 tttAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAACAATCAGGCTTGCCGATCCCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGAACAAGACCACTCAGGAACTc                                                                                                                                                                                                                                                     >  1:143451/1‑299 (MQ=255)
                                                       gcccgatcatactgcgccCGTTTGGTGTGACAACCAATAGTTTTCGTCGAGAGCCACAGGGCGATTGTCATAATGCTTACACCCGAGATTAAGGCGCGGAACCGATTTCCACGATGCACGCCTACCCCCATCGCCCCGAACAAGCCAGTGGTGGCAAGATCGCCAAACGGCAGCACCTGACTACCCGGCACACAAACAGCAATTAGAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTTAcc                                                                                                                                                                                                                                               <  1:342155/285‑1 (MQ=255)
                                                        ccccAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGGGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAACTGGTACGTGGCATACAACTCATGCTTACAGATACGA‑‑CCGCCGTATGTCCCCGCAGCACCGCGGGATCAAGACCCAACCCGAACTCCTAAccgc                                                                                                                                                                                                                                             >  1:303836/1‑300 (MQ=255)
                                                            cgCATTATCCGCCCGTTCCGTGTGCAAACCAATAGTGTGCGTCGCGATCCCCCGGCTGATGCTCATAATGATGACACCCCAGATTAAGTTGCGGAACAGCTTTCCACGATGCCCGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAACATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCCGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAAc                                                                                                                                                                                                                                        <  1:269046/300‑1 (MQ=255)
                                                                            ttggTGTGCAAACCAATAGTGTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGAc                                                                                                                                                                                                                        <  1:188539/299‑1 (MQ=255)
                                                                                        ccAATAGTTTGAGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCAAGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACCGCAATTAAAATGGTTAGTGGGATAAAAATCAGGATTGCCGATACCA‑‑CCGCCGTATGTACCAGCAGCAACGCCGGACACAGGCCCATCAGGACATCATGACAGCCGACCTTAGACTGTAACAGACTACGAGcagtca                                                                                                                                                                                                            >  2:95762/1‑296 (MQ=255)
                                                                                              ttgtGCGTGGCGAGCCAAAGGGGGATGCGCATAAGAAGGACACCCGAGATAAAGGTGCGGAACAGATTTCCACGAGGAACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGa                                                                                                                                                                                                      <  2:133974/298‑1 (MQ=255)
                                                                                              ttgtGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCTTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGa                                                                                                                                                                                                      <  1:437646/298‑1 (MQ=255)
                                                                                               tttGCGGCGCGATCCACAGGGCGATGATCAAAATGATGACACCCGAGATTAAGGTGAGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCg                                                                                                                                                                                                       <  1:60437/299‑1 (MQ=255)
                                                                                                       gcgATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGACGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATGACACGGCACACAAACAGCAATTAAAATGGTGAGTGGGATACAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCCACGCCGGATCAAGGCCAATCCGGAACTCCTGACCGACGAACTTCGCCTGTAAACGACTACGAGACTGCTTAGCGATGGGCGTTa                                                                                                                                                                                             >  1:126701/1‑301 (MQ=255)
                                                                                                          atcccaaGGGTGATGATCATAAGGATGACACCCGAGAGTAAGGGGCGGAACAGAGTGCCAAGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAATATCGCCAAACGTCAGCACCTGATTACCCGGCACACAAACCGCAATTAAAATGGTGAGTGGGATAACAAGCAGGCGTGCCGATAC‑‑CCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAc                                                                                                                                                                                          <  1:242477/295‑1 (MQ=255)
                                                                                                          aTCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGATGCGGATCAGATTTCCACGATGCACGGCGACCGCCTTAGCCACGGAGAAGACAATGATGGCAAAAACGCCACACGGCAGCACCTGCTTACCATGCACACAACCAGCAAGTAAAATGTTGAGACAGCTAAAAATAAGGCTTGCCGAAACTA‑‑CCGCCGTAAGTCCCAGCAGCAGCGGAGAATAAAGAGCAACACGAAAGTCCTGAACGGCGAACTTCTCCGGTAAAGTACTAGGAGACTACTTCACGCTGGACGCTaac                                                                                                                                                                                           >  1:639287/1‑299 (MQ=255)
                                                                                                            ccACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAACAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGGCCCAGCAGCAACGCCGGATCAAGGCCAAGCAGGAACTCCTACCAGCCGAACTTCGCCAGTAACCGACTACGAGCCTGCTTAACGATGGGCGTGAACcca                                                                                                                                                                                        >  1:395833/1‑301 (MQ=255)
                                                                                                            ccACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAAAAGCAATTAAAATGGTGAGAGGGATAAAAATCAGGCTTGACGATAC‑‑CCCCGCCGTATGTCCCAGACGCAAAGCCGGATCAAGGCACATCAGGAACTCCTGACCGCCGCCATTCGCCTGTAAACGAACACCGACATGCTTAGCGATGGGCGTGAAAcca                                                                                                                                                                                        >  1:485968/1‑301 (MQ=255)
                                                                                                            ccACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGACACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCAACTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGAAAGGCCGGATCAAGGCCAATCAGGAACTCATGACCAGCGCACTTCGCCGGTAAACGAACACGAGCCTGCTTAGCGATGGACCTTaaaac                                                                                                                                                                                         >  1:60348/1‑298 (MQ=255)
                                                                                                                 gggTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCCAACGGCAGCACCTGATTACCCGGCACGCAAACAGAAATTAAAATGGTAAGTGGGATAAAAATCAGGATTGCCGATACCA‑‑CCTCCGTATGTCCAAGCAGCAACGCCGGATCAAGGAAAATCCGGAACTCCTGCCCTCCGGACTTCGCCTGTAAACGACTACAGGCCGGCTTAACCAGTGGCCTTAAAcactccc                                                                                                                                                                                    >  1:98867/1‑294 (MQ=255)
                                                                                                                       tGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGAATCAAGGCCAAACAGGAACTCCTGACCGACGACCTTCGCCTGTACACGACTCACAGCCTGCTTAGCGATGGGCGTTAACCACCCCATGCTggg                                                                                                                                                                             >  2:638659/1‑301 (MQ=255)
                                                                                                                              aaTGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGa                                                                                                                                                                                                      <  1:394442/269‑1 (MQ=255)
                                                                                                                               actatgaCACCCGAGATTAAGGTGCAGAACAGATTTCCACGAGGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATAC‑‑CCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCa                                                                                                                                                                     <  1:711081/298‑1 (MQ=255)
                                                                                                                               aagaagaCACCCAAGATTAAGGGTAGGAACGGAGTTCCACGAAGCACGACGAACGCCATCGCCACGAAGAAGCAGATGGTGACAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAAGTAAAATGGTGAATGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCAGATCAAGGCCAATCGGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCa                                                                                                                                                                     <  1:241334/296‑1 (MQ=255)
                                                                                                                                            agaTTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGGTAAACCATCCATGCTGGGTTTAATCACCCGTGGCATTag                                                                                                                                                        >  1:443797/1‑301 (MQ=255)
                                                                                                                                             gaTTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTACTCACCCGTTGCATTac                                                                                                                                                        >  1:437838/1‑299 (MQ=255)
                                                                                                                                                     tcgggaaaagaatgcccagcatcagggCGACCCACAGCGCAACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGACTACCCGGCACACCAACAGCACTTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCTCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCAc                                                                                                                                               <  2:569399/276‑1 (MQ=255)
                                                                                                                                                        cgAACACATTGCCAAGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTTATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTgc                                                                                                                                            <  2:437827/300‑1 (MQ=255)
                                                                                                                                                         gAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAATATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCCTTAAACCATCCCTGATGGGTTTAATCCCCCGTGTCATTCTCAGCATACCTgc                                                                                                                                            >  2:557818/1‑300 (MQ=255)
                                                                                                                                                              gatggcccagATGCACGGCGACCGACATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGc                                                                                                                                                                                                 <  2:180105/233‑1 (MQ=255)
                                                                                                                                                              gATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGc                                                                                                                                                                                                 >  1:180112/1‑242 (MQ=255)
                                                                                                                                                              gATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGACCCTGCTTATCGATGGGCGTTAAACCACCCCTGATGGTTTTAACCCCCCGTGCCATTGTCACGCTCACCGCCGCCGt                                                                                                                                      >  2:7207/1‑301 (MQ=255)
                                                                                                                                                              gATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGCTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCCCACAAACAGCAATTAACATGGTGAGTGGGCTCCAACTCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCACCAGCAACGCCGGATCACGGCCATTCCGGAACTCCTGACCGCCGCACTTCGCCTGTACACGACACCGACCCTGCTTCGCAATGGGCGTTAACCCCTCCATGCTGGTTTTATTTCCCCGTGGCATTACCCGCACCCCTCccaccgg                                                                                                                                      >  2:442253/1‑300 (MQ=255)
                                                                                                                                                                        ttccACGGCGATCCCAATCGCACCCAATACGTCGTTGATAGCAAGACCGCCAAACAGCAGCACCTGATTATCCGGCACACAAACCGCAATTAAAATGGTGAGTTGGTTAAAAACCAGGTTTGCCGACACCA‑‑CCGCCGTAGGTCCCAGCAGCAACGCCGGATCAAGTCCAATCAGTAACTCCTGACCGCCGAACTTCTCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGcc                                                                                                                            <  1:294865/298‑1 (MQ=255)
                                                                                                                                                                        aTGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGACCTTCGCCTGTACACGACTACGAGCCTGATTAGCGATGGGCGTTACACCATCCATTATGGGTTTACTCACCCGTTGCCTTAGCAGCATCCCTGCCCCCGtttttcaccc                                                                                                                             >  2:558703/1‑295 (MQ=255)
                                                                                                                                                                        aTGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTCGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCCACACTGCCGCAGTTTTTCcccc                                                                                                                             >  2:279261/1‑298 (MQ=255)
                                                                                                                                                                        aTGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCTTCACTGCCGCCGTTTTTACCGc                                                                                                                             >  1:428546/1‑300 (MQ=255)
                                                                                                                                                                                   ccGCCATCGCCACGAAGAAGCAGAGGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATac                                                                                                                 <  1:62803/301‑1 (MQ=255)
                                                                                                                                                                                   ccGCCACCGCCACAAAAAAGCCGATGGTGGCAAGATCGCCAAACGGCAACACCTGATTACCCTGCACACAAACAGCAATGAAAAGGGGGAGTGGGATCAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATac                                                                                                                 <  2:443784/301‑1 (MQ=255)
                                                                                                                                                                                   ccGCAAACGCAACGAAGAAGCAGATGGGGGAAAGATCGCAAAACGGCAGCAACTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAGAAAAATCAGGCTTGCCGATAC‑‑CGCCGCCGTATGTCTCAGCAGGAACGCCGGATCAAGGCCAATCAGGAACTCCTTACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATac                                                                                                                 <  2:303830/301‑1 (MQ=255)
                                                                                                                                                                                     ctcggaaagacgtataatagacaggtgtCAAGCTCGCCAACCTCCAGCACCTTATTACCCGACCCACAAAAAGCATTTAATATTGTGAGTGCGATAATAATCAGGCTTCCCGATACCA‑‑CCTCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGCCCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCTTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATacac                                                                                                               <  1:190691/278‑1 (MQ=255)
                                                                                                                                                                                     cccatccgcacagagaagCCGATGGTGGCAAGAACGCAAAACGGCAGCACCTGAGTACCCGGCACAAAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATacac                                                                                                               <  1:31265/288‑1 (MQ=255)
                                                                                                                                                                                            ccacagagaagCCGAAGGGGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGAACACAAAAAGCAAGTGAAATGGTGAGTGGGATAAAAATCAGGCTCGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATAAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTg                                                                                                          <  2:428535/293‑1 (MQ=255)
                                                                                                                                                                                              aagaaaaaGCCGAGGGGGGCAAAATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCGGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCACCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACAt                                                                                                      <  1:7208/299‑1 (MQ=255)
                                                                                                                                                                                                   gaagCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTAAGCGATGGGCGTTAAACCATCCATGATGGGTTTACTCACCCGTGGCATTAGCGGCAACACTGCCGCCGTTTTTACCGCCGGCCGCAATCCCACTTTAACCTCGTaa                                                                                                 >  1:600864/1‑301 (MQ=255)
                                                                                                                                                                                                   gaagCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTAGCCTGTAAACGACTACGAGCCTGCTTACCGCTGGCCGTTAAACCATCCATGCTGGGTTTAATCACCCGTGTCATTAGCCGCCTCCCTGCCCCGGTTTTTACCTCAAGCCGCAATCCACCttgccccttctaa                                                                                                 >  2:227842/1‑290 (MQ=255)
                                                                                                                                                                                                       ccGATGGTGGCAAGAGCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAAcc                                                                                               <  2:221064/299‑1 (MQ=255)
                                                                                                                                                                                                              tggCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGAt                                                                                      >  1:195860/1‑301 (MQ=255)
                                                                                                                                                                                                                        cacAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTTGGATAAAAATCATGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTTACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATc                                                                            <  1:558726/299‑1 (MQ=255)
                                                                                                                                                                                                                             aacgcagaaCCCGATTACCCGGCACACAATGATCACTTAAAATGTTGAGTGGGATAAAAATCAGGCCTGCCGATACCA‑‑ACTCCGTATGTCCAAGCAGCAACGCCGGATCAATGCCAAGCAGGCACTCTTGACCGCCGAACTTCGCCTGTAATCGCCTTCGAGCCTGCTTGGCGATGGGCGTTCAACCATCCATGATGGGGTTAATCACCCGTGGTATTAGCAGCATCACTGCCGCCTTTTTTACCGCCAGCTGCAATACCCCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGccc                                                                       <  1:463210/298‑1 (MQ=255)
                                                                                                                                                                                                                                      ccggagcaCCCGGCACCAAAACAGCAATTAAAATGGTGAGTGGGAGAAAAACCAGGCTTGCCGATACCA‑‑CCGCCGTAGGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACaaa                                                              <  1:557841/294‑1 (MQ=255)
                                                                                                                                                                                                                                        tGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACAACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCAGCCGTTTTTCCCGCCAGCTGCAATACACCTTTGACCTCGTAACCCGCGAGGATGCCGATACTCATGCCCCTcacacaca                                                            >  1:679196/1‑297 (MQ=255)
                                                                                                                                                                                                                                         taatacacagcaaagaaaCACAAAGTAAAATGGGGAGGGGGATAAACATCAGGCGTGCCGCTCCCA‑‑CCGACTGATGTCCCAGCAGCAGCGCCGGATCAAGGCCAATCAGGAACTCCGGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTGTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTCTTTACCGCCAGCTGCAATACACCTTTGACAGCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAAcc                                                           <  2:98863/286‑1 (MQ=255)
                                                                                                                                                                                                                                                  gCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCAATGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGCTAACAAGCCCCATCACAAAACCCACGGtca                                                   >  1:518937/1‑298 (MQ=255)
                                                                                                                                                                                                                                                   cacacaAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCAGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTAGCCTGTAAACGCCTACGAGCCTGCTTAGCGATGAGCGTTCAACAATCCATGATGGGTTTAATCACCCGTGGCCTTCGCAGCATCACTGACGCCGTTTTTCCCGCGAGCTGCCATCCACCTTTGCCATCGTCACCCGCGAGGCTCCCGATAATCAGACCCAtaccacaacacccgttcac                                                  >  1:644554/1‑282 (MQ=255)
                                                                                                                                                                                                                                                      tgatagcccacgagcgtggcggAGTGGGATAAAAATGTGGTGTCCCTATAC‑‑CCCCGCCGTCGGCCCCAGCAGCCGCTCCTGAGCAAGGACAAACAGCAACTCCTGACCGCCCAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCAGGATGGGTTTAATCACCCGTGGCAGTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGc                                              <  2:660548/280‑1 (MQ=255)
                                                                                                                                                                                                                                                      acaAACAGCAATGAAAATGGTGAGTGGAATAAAAATCAGGCCTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCAGCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGc                                              <  1:279267/301‑1 (MQ=255)
                                                                                                                                                                                                                                                           cAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCCTCACAAACCCCACGGTGACAGGCCCGc                                          >  2:251163/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                   aaaaTGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCTTGGCATTAGCAGCATCACTGCCGCCGTTTTTCCCGCCAGCTGCAATACACCTTTGACCTCGTAACCCGCGAGGCTTCCGATAATCAGCCCCATCACAACAACCCCGGTGCCCGGCTCGCCACAtcgc                                  >  2:706369/1‑297 (MQ=255)
                                                                                                                                                                                                                                                                                ggATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGGTAATCAGCCCCATCAAAAAACCCACGGTGCACGGCTCGCCCAATAGACCAAATTTgcgca                     >  2:169249/1‑299 (MQ=255)
                                                                                                                                                                                                                                                                                 gATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCGGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGCTGCCAGTCTAGCCAAATGTACCAAATTTGCGCTGa                   >  1:504409/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                  aTAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATTAACCTTTGACATCGTAACCCGCGAGGTTGCCGGTAATCAGCCCCCTCACAAAACCCACGGTGACAGGATCCCCAAACAGACCAAATTTCCGCTGaa                  >  1:283939/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                      aaaTCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGCGGATGCCGATAATCAGCCCCATCACAACACCCACGGTTACAGGCTCCCCAAATGGACCCAATTTCCGCtcgataa               >  1:610680/1‑294 (MQ=255)
                                                                                                                                                                                                                                                                                                tGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTCACAGGCTCGCCAAAGGAACCAAATTTGCCCTGAATATCGTCGgcgcca    >  2:543912/1‑299 (MQ=255)
                                                                                                                                                                                                                                                                                                  ccGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCCCTTCCGACGTTTTTACCGCCAGCTGCACTACACCTTTGACATCGTACCCCGCGAGGATGCCGATAATCAGCCCCATCCAAAAACCCCAGGTGCAAGCCTCCCCAAATGCAACAAATTTTCACTGAATTtctaccccacaaaa  >  2:567470/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                                           ||                                                                                                                                                                                                                                                                                                    
GGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAA  >  NC_000913/2173062‑2173654

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.

GATK/CNVnator alignment

N/A