Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F17 I0 R1	115	121.7	2441348	96.4%	2353459	231.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,282,677	T→G	14.6%	G938G (GGT→GGG)	narG →	nitrate reductase 1, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,282,677	0	T	G	14.6%	190.6 / 2.1	83	G938G (GGT→GGG)	narG	nitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand): ref base T (31/34); new base G (12/3); total (44/39)
Fisher's exact test for biased strand distribution p-value = 4.20e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTG‑CCTGGAACACCCAGAGCG‑AGATGGATCTGCTGCGTAAGCTCAACTACA‑CCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAG‑ATGATCCTGAC‑ACTGGCACCGGAAACCAACGGTCAGGTA‑GCCGTGAAAGCCTGGG‑CTGCCCTGAGC‑GAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGG  >  NC_000913/1282440‑1282912

tGAACTGGCGCAGCCGCTGGATGTGAAAGACTGAAAAAAAGGCGAGTGCGACCTGATCCCAGGTAAAGCCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTG‑CCTGGAACACCCAGAGCG‑AGATGGATCTGCTGCGTAAGCTCAACTACA‑CCAAAGCGGAAGGt                                                                                                                                                                                                                                                  <  2:421967/238‑1 (MQ=255)

          cAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTG‑CCTGGAACACCCAGAGCG‑AGATGGATCTGCTGCGTAAGCTCAACTACC‑CCAAAGCGTAAGGTCCGGCGAAAgg                                                                                                                                                                                                                                       <  2:1161190/239‑1 (MQ=255)

            gCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGGATG‑CCTGGGACACCCACAGCG‑AGCTGGATCTTCTGGGTAAGGTCCAATACA‑CCCAACCCGAAGGGCCGGGGAAAGGc                                                                                                                                                                                                                                      >  1:797768/1‑238 (MQ=255)

               gCTGGAAGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCAGGTAAAACCGCGCCACACATTATGGTCGTGGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTG‑CCTGGAACACCCAGAGCG‑AGATGGATCTGCTGCGTAAGCTCAACTACA‑CCAAAGCGGAAGGTCCGGCGAAAGTCCAGc                                                                                                                                                                                                                                  <  2:644967/239‑1 (MQ=255)

                 tGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTG‑CCTGGAACACCCAGAGCG‑AGATGGATCTGCTGCGTAAGCTCAACTACA‑CCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCg                                                                                                                                                                                                                                <  1:329142/239‑1 (MQ=255)

                 tGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTG‑CCCGGAACACCCAGAGCG‑AGATGGATCTGCTGCGTAAGCTCCAACACC‑ACAAAGCGGAAGGGTCGGCGAAAGGcacgacg                                                                                                                                                                                                                                >  1:1067477/1‑233 (MQ=255)

                  ggATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTG‑CCTGGAACACCCAGAGCG‑AGATGGATCTGCTGCGTAAGCTCAACTACA‑CCAAAGCGGAAGGCCCGGCGAAAGGCCAGCCGa                                                                                                                                                                                                                               <  1:10467/239‑1 (MQ=255)

                          aaGACTGGAAAAAAGGCGATGGCGACCTGATCCCAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTG‑CCTGGAACACCCAGAGCG‑AGATGGATCTGCTGCGTAAGCTCAACTACA‑CCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGaa                                                                                                                                                                                                                        <  1:563649/238‑1 (MQ=255)

                               tGGAAAAAAGGCGAGTGCGACCTGATCCCAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGAAAAAGGATTG‑CCTGGAAAACCCCAAGGG‑AGATGGATCTTCGGCGTAAGCTCAACTACA‑CCACATCGGAAGGGCCGGGGAAAAGCCGCCCGAtcatacaacccgc                                                                                                                                                                                                                  >  2:854391/1‑227 (MQ=255)

                                       aggggggTGCGACCTGATCCCAGGTAAAACCGCGCCACGCATTAGTGTCGTAGGGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTG‑CCTGGAACACCCAGAGCG‑AGATGGATCGGCTGCGTAAGCTCAACTACA‑CCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATg                                                                                                                                                                                                          <  2:856242/233‑1 (MQ=255)

                                        ggCGAGTGCGACCTGATCCCAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGACCCGGAA‑ACCCAGAGCG‑CGATGGGGCTGCGCCGTGAGACCAAATACA‑CCAAAGCGGGAAGGGCGGGGAAAAGCCAGCCGGTGCGGCACAccaccaatggtcg                                                                                                                                                                                                         >  2:861889/1‑228 (MQ=255)

                                            aGTGCGACCTGATCCCAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTG‑CCATGCACAACCAGAGCG‑AGCGGGATCGCATGCGTAGGATCAACTACA‑CCAAAGGGGAAAGAGCGGGGGAAGGCGCGCCGATGCTGAACAACGCAACTGGTggcggc                                                                                                                                                                                                     >  2:114518/1‑234 (MQ=255)

                                                 gaacagATCCCAGGTAAAACCGCGCATCTCATTGTGGTCGTGGAGCGCGATTAGCAGGCGACGTACGAACGCTTTACCTCTATCGGCCCGCAGATGGAGAAAATCGGTAATGGCGTTAAAGGGATTG‑CCTGGAACACCCAGAGCG‑AGATGGATCTGCTGCGTAAGCTCAACTACA‑CCAAAGCTGAAGGTCCGGCGAAAGTCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAg‑at                                                                                                                                                                                               <  2:1193803/234‑1 (MQ=255)

                                                        tCCCAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTG‑CCTGGAACACCCAGAGCG‑AGATGGATCTGCTGCGTAAGCTCAACTACA‑CCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAG‑ATGATCCt                                                                                                                                                                                         <  1:760674/238‑1 (MQ=255)

                                                        tCCCAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCGATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTG‑CCTGGAACACCCAGAGCG‑AGATGGATCTGCTGCGTAAGCTCAACTACA‑CCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAG‑ATGATCCt                                                                                                                                                                                         <  1:114518/238‑1 (MQ=255)

                                                             ggTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAAGGATTG‑GCTGGTACCCCCCGAGCG‑AGAGGGGTCTGTTTCGTAAGTCCAACTCCA‑CCCAAGCGGAACGTGCGGCGGAAGGCCCCCCGGGGCCGAACACCGCACATGACGCggtcagggtggtgctggc                                                                                                                                                                                       >  2:64069/1‑220 (MQ=255)

                                                                aaaaCCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTG‑CCTGGAACACCCAGAGCG‑AGATGGATCTGCTGCGTAAGCTCAACTACA‑CCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAG‑ATGATCCTGAC‑ACTgg                                                                                                                                                                                <  2:122460/238‑1 (MQ=255)

                                                                   aCCGCGCCACACATTATGGTCGTAGCGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTT‑CCCGGAACACCCAGAGCG‑AGATGGATCTTCTGCGTTAGCTCAACCACACCCAAACCGGAAGTGCCGCGGAAGTGCCA‑CCGCTGCCGTACACCCGAATTTATGAGGGCGAG‑GAGATCCTCACAACTGGcca                                                                                                                                                                             >  2:794299/1‑237 (MQ=255)

                                                                            cacaTTAGTGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTGTACCTCTATCGGCCCGCTGATGGAGAAAATGGGTAATGGCGGTAAAGCGATTG‑CCTGGAACACCCAGAGCG‑AGATGGATCTGCTGCGTAAGCTCAACTACA‑CCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAG‑ATGATCCTGAC‑ACTGGCACCGGAAACCaa                                                                                                                                                                   <  2:129546/239‑1 (MQ=255)

                                                                                 tatgagcGTAGCGCCAGATTATCCGGCGACTTACGTACGCTTTACCTCTATCGGGCCGCTGACGGAGAAAATCGGTAATCGCGGTAAAGGGATTG‑CCTGGAACACCCAGAGCG‑AGATGGATCTGCTTCGTAAGCTGAACTACA‑CCAAAGCGGAAGGCCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGTG‑ATGATCCTGAC‑ACTGGCACCGGAAACCAACGGTc                                                                                                                                                              <  2:467427/233‑1 (MQ=255)

                                                                                       cGTCGAGCGCTATTATCCGGCCACTTTCGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTG‑CCTGGAACACCCAGAGCG‑AGATGGATCTGCTGCGTAAGCTCAACTACA‑CCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAG‑ATGATCCTGAC‑ACTGGCACCGGAAACCAACGGTCAGGTa                                                                                                                                                         <  2:45215/238‑1 (MQ=255)

                                                                                       cGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTG‑CCTGGAACACCCCGAGCG‑AGATGGATCTGCTGCGTAAGCTCAACTACA‑CCAAAGCGGAAGGGCCGGCGAAAGGCCAGCCGATGCTGAACACCCCAATTGATGCGGCAGAG‑ATGATCCTGAC‑ACTGGCACCGGGAACCAACGGTCAggga                                                                                                                                                         >  1:956217/1‑236 (MQ=255)

                                                                                             gcgcGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTG‑CCTGGAACCCCCAGAGCG‑AGATGGATCTGCTGCGTAAGCTCAACTACA‑CCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAG‑ATGATCCTGAC‑ACTGGCACCGGAAACCAACGGTCAGGTA‑GCCGTg                                                                                                                                                  <  1:642964/238‑1 (MQ=255)

                                                                                              cgcgATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAATGGATTT‑CCTGGGACCCCCCGGGCG‑GGATGGATCTTGTGCGGAAGCTCAAATACC‑CCCAAACGGAAGGGCCGGCGAAAAGACCGCCGCTGATGAACACCCCAATTGATGCGGCCGAG‑ACTAACCCGGC‑ACCCGCCCCGGAAACCAACGGTCCGGCA‑GGCgctga                                                                                                                                                >  2:239717/1‑235 (MQ=255)

                                                                                                 gATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAAGCGGTAATGGCGGTAAAGGGATTG‑CCTGGAACACCCAGAGCG‑AGATGGATCTGCTGCGTAAGCTCAACTACA‑CCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAG‑ATGATCCTGAC‑ACTGGCACCGGAAACCAACGGTCAGGTA‑GCCGTGAAAGc                                                                                                                                             <  1:466926/239‑1 (MQ=255)

                                                                                                            gACTTACGAACGCTTTACCTCCACCGGCCCGCTGATGGAGAAAAACGGTAATGGCGATAAAGGGATTG‑CCTGGAACACCCAGAGCG‑AGATGGATCTGCTGCGTAAGCTCAACTTCA‑CCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAG‑ATGATCCTGAC‑ACTGGCACCGGAAACCAACGGTCAGGTA‑GCCGTGAAAGCCTGGG‑CTGcc                                                                                                                                  <  1:353251/238‑1 (MQ=255)

                                                                                                                         ttaaCCTGTATCGTCCCGCTGATGGAGAAAATCGGTAAGGGGGGTAAAGGGATTG‑CCTGGAACACGCAGGGCG‑AGATGGATCTGCGGCGTAAGCTCACATACA‑CCAAAGCGGAAGGGCCGGCGAAAGGCCGGCCGATGCTGAACACCGCAATTGATGCGGCAGAG‑ATGATCCTGAC‑ACTGGCACCGGAAACCAACGGTCAGGTA‑GCCGTGAAAGCCTGGG‑CTGCGCTGAGC‑GAAGTTa                                                                                                                    <  2:1198689/235‑1 (MQ=255)

                                                                                                                           tACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAAGGATTG‑CCTGGAACACCCAGAGCG‑AGATGGGTATGCTGCGTAAGCTCAACTACA‑CCAAAACGGGAGGGCCGGCGAAAGGCCAGCCGATGCTGAACACCCCAATTGGAGCGGCAGGG‑AAGATACTGAC‑CCTGGCCCCCGGAAACAACGGTCCGGGA‑AGCGTGAAAGCCCGAG‑CTTCCCCCAGC‑GGAtttcacc                                                                                                                 >  2:354031/1‑235 (MQ=255)

                                                                                                                                 tATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTG‑CCTGGAACACCCAGAGCG‑AGATGGATCTGCTGCGTAAGCTCAACTACA‑CCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAG‑ATGATCCTGAC‑ACTGGCACCGGAAACCAACGGTCAGGTA‑GCCGTGAAAGCCTGGG‑CTGCCCTGAGC‑GAATTTACCGGTCGTg                                                                                                           <  1:249562/239‑1 (MQ=255)

                                                                                                                                 tATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAAGGATTG‑CCTTGAACACCCAGAGCG‑AGATGGAACTGCTGCGTAAGCTCAACTACA‑CCAAAGCGGAAGGGCCGGCGAAAGGCCAACCGATGCTGAACACCCCCATTGATGCGGCAGGG‑AGGATCCTTAC‑AACGGCCCCCGCAACCAACCGTAAGGGA‑GGCGGGAAAACCCCGG‑CTGCCCTGGGC‑GCAttttacgccggtc                                                                                                           >  2:1008542/1‑229 (MQ=255)

                                                                                                                                   tCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTT‑GCTGGGACACCCAAAGCG‑GGAGGGATCTTGCGCGTAAGCTCAACTACA‑CCCAAGCGGGAGGGCACGGGAAACGCCCGCCGAGGCCTCATCCCGCCATTGCTGAGGCCGGGCATAACCTCGCC‑CCGGGCCCCGAAAACAAAC‑GCCAGGTAGGCCGGAAAAGCC‑GGG‑CCGCCCTCAGC‑GGATTTAACCGTCggca                                                                                                          >  2:1073002/1‑235 (MQ=255)

                                                                                                                                   tCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTG‑TCTGGGACACCCCGAGCG‑AGATGGGTCTGCTGCGGAAGCGCAACTACA‑CCAAAGCGGAAGGGCCGGCGGAAGGCCCGCCGCTGCTGCACACCGCCATTGATGCGGCCGAG‑ATGATCCTGCC‑ACCGGCACCCGAAACCAACGGTCAGGGA‑GCCGGGAAAACCTGGGCCTGCAC‑GAGCGGAATTTACCGGtgcgg                                                                                                           >  2:899405/1‑234 (MQ=255)

                                                                                                                                       cccGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGACTG‑CCCGGAAAACCCCAAGCG‑AGAGGGATCTGGCGCGTAAGCGCCAATACA‑ACAAAACGGGAGGTCCGGCGCAAGGCCCCCCGACGCGGAACACCCCAAATGAAGGGGCAGGG‑GCGAGACCGGC‑ACTGGCACCCGGAACAAACGGTCAGGTG‑AGCGGGAAAACACGGG‑GTGACCTGAGC‑GACATTAcccggagggacccca                                                                                                     >  2:540118/1‑226 (MQ=255)

                                                                                                                                       cccGCTGATGGAGAAAATCGGTAATGGCGGAAAAAGGATTG‑CCCGGGACACCCAGAGCG‑AGATGGGTCTGCTGCGTAAGCTCAACTAAA‑CCAAAGCGGAAGGGCCGGCGAAAGGCCCGCCGATGGTGAAAACCCCAATTGGAGCGGCAGGG‑GTGATCCTGAC‑ACTGGCACCGGGAACCAAAGGTCCGGGA‑GCCGGGAAAGCCTGGG‑CTGGCCCGGGG‑GAAttttacgggcggggcccg                                                                                                      >  2:35527/1‑223 (MQ=255)

                                                                                                                                           cTGATGGAGAAAATCGGTAATGGCGGTAAAAGGATTG‑TCGGGAAAAACCCGCGCGAAGAGGGA‑CTGAGGCGGAAGCTCGACGCCA‑CCAAAACGGAAAGTGCGGCGGAAGGCCAGCCGATGCTGTACACCGCAACTGATGCGGCAGAG‑AGGATCCTGCC‑ACTGGCACCGGAAAACCACGGTGAAGTA‑CCAGGGGAAACCCGGG‑CTGCCACCGGA‑GGATATACCCGTCGTGAcgctaacc                                                                                                  >  2:1136400/1‑231 (MQ=255)

                                                                                                                                                   gaAAATCGGTAATGGCGGTAAAGGGATTG‑CCTGGAACACCCAGAGCG‑AGATGGATCTGCTGCGTAAGCTCAACTACA‑CCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAG‑ATGATCCTGAC‑ACTGGCACCGGAAACCAACGGTCAGGTA‑GCCGTGAAAGCCTGGG‑CTGCCCTGAGC‑GAATTTACCGGTCGTGACCATACGCATCTGgcg                                                                                          <  1:323032/238‑1 (MQ=255)

                                                                                                                                                                  cGGTAAAGGGATTG‑CCTGGAACACCCAGAGCG‑AGATGGATCTGCTGCGTAAGCTCAACTAAA‑CCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAG‑ATGATCCTGAC‑ACTGGCACCGGAAACCAACGGTCAGGTA‑GCCGTGAAAGCCTGGG‑CTGCCCTGAGC‑GAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGAc                                                                           <  1:1136400/238‑1 (MQ=255)

                                                                                                                                                                               g‑CCTGGAACACCCAGAGCG‑AGATGGATCTGCTGCGTAAGCTCAACTACA‑CCAAAGCGGAAGGTCCGGCGAAAGGCCACCCGCTGCTGAACACCGCAATTGATGCGGCAGAG‑ATGATCCTGAC‑ACTGGCACCGGCAACCAACGGTCAGGTA‑GCGGTGAAAGCCCGGG‑CTGCCCTGGGC‑GCATTTACAGGTCGCGACCATACGCATCTGGCGCTGACTAAAGAGGCCGAGACGATCCgac                                                              >  2:221548/1‑236 (MQ=255)

                                                                                                                                                                                 ccTGGAACACCCAGAGCG‑AGATGGATCTGCTGCGTAAGCTCAACTACA‑CCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAG‑ATGATCCTGAC‑ACTGGCACCGGAAACCAACGGTCAGGTA‑GCCGTGAAAGCCTGGG‑CTGCCCTGAGC‑GAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCtt                                                             >  1:389002/1‑238 (MQ=255)

                                                                                                                                                                                   tGGAACACCCAGAGCG‑AGATGGATCTGCTGCGTAAGCTCAACTACT‑CCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATGGATGCGGCAGAG‑ATGATCCTGAC‑ACTGGCACCGGAAACCAACGGTCAGGTA‑GCCGTGAAAGCCTGGG‑CTGCCATGAGC‑GAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCcg                                                          <  1:461273/239‑1 (MQ=255)

                                                                                                                                                                                    ggAACACCCAGAGCG‑AGATGGATCTGCTGCGTAAGCTCAACTACA‑CCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAG‑ATGATCCTGAC‑ACTGGCACCGGAAACCAACGGTCAGGTA‑GCCGTGAAAGCCTGGG‑CTGCCCTGAGC‑GAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCcgc                                                         <  1:1025273/239‑1 (MQ=255)

                                                                                                                                                                                    ggAACACCCAGAGCG‑AGATGGATCTGCTGCGTAAGCGCAACTACA‑CCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAG‑ATGATCCTGAC‑ACTGGCACCGGAAACCAACGGTCAGGTA‑GCCGTGAAAGCCTGGG‑CTGCCCTGAGC‑GAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCcgc                                                         <  1:1017095/239‑1 (MQ=255)

                                                                                                                                                                                    ggAACACCCAGAGCG‑AGATGGATCTGCGGCGTAAGCTCAACTACA‑CCAAAGCGGAAGGTCCGGCGAAGGGCCAGCCGATGCTGAACACCGAAATTGATGCGGCAGAG‑ATGATCCTGAC‑ACTGGCACCGGAAACCAACGGTCAGGAA‑GCCGTGAAAGCCTGGG‑CTGCCCTGAGC‑GAATTTAGCGGTCGTGACCATCCGCATCTGGCGCTGAGTAAAGAAGCCAAGAACATCGGCTTCcg                                                          >  2:698673/1‑238 (MQ=255)

                                                                                                                                                                                       acacCCAGCGCG‑AGACGGATCTGCTGCGTAAGCTCAACTACA‑CCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAG‑ATGAACCTGAC‑ACTGGCACCGGAAACCAACGGTCAGGTA‑GCCGTGAAAGCCTGGG‑CTGCCCTGAGC‑GAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGa                                                       <  2:460867/238‑1 (MQ=255)

                                                                                                                                                                                           ccAGAGCG‑AGATGGATCTGCTGCGTAAGCTCAACTACA‑CCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAG‑ATGATCCTGAC‑ACTGGCACCGGAAACCAACGGTCAGGTA‑GCCGTGAAAGCCTGGG‑CTGCCCTGAGC‑GAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTc                                                  >  1:335522/1‑239 (MQ=255)

                                                                                                                                                                                                gCG‑AGATGGATCTGCTGCGTAAGCTCAACTACA‑CCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAG‑ATGATCCTGAC‑ACTGGCACCGGAAACCAACGGTCAGGTA‑GCCGTGAAAGCCTGGG‑CTGCCCTGAGC‑GAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGc                                              >  2:958787/1‑238 (MQ=255)

                                                                                                                                                                                                    agaTGGATCTGCTGCGTAAGCTCAACTACA‑CCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAG‑ATGATCCTGAC‑ACTGGCACCGGAAACCAACGGTCAGGTA‑GCCGTGAAAGCCTGGG‑CTGCCCTGAGC‑GAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGcaca                                           >  1:640862/1‑238 (MQ=255)

                                                                                                                                                                                                     gaggggtcggctgcCTAAGCTCAACGACA‑AGAATGCGGAAGGGGCGGGGAAAGGCCGGCCGATGCTGAACACCGCAATTGATGGGGCAGAG‑ATGATCATGAG‑ATTGGCACCGGAAACCAACGGTCAGGTA‑GCCGTGAAAGCCTGGG‑CTGCCCTGAGC‑GAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAg                                          <  2:797768/229‑1 (MQ=255)

                                                                                                                                                                                                      aTGGATCTGCTGCGTAAGCTCAACTACA‑CCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAG‑ATGATCCTGAC‑ACTGGCACCGGAAACCAACGGTCAGGTA‑GCCGTGAAAGCCTGGG‑CTGCCCTGAGC‑GAATTTACCGGTCGTGACCATCAGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCCTCCGCGATCTTCAGGCCCCGcc                                        >  2:862353/1‑239 (MQ=255)

                                                                                                                                                                                                      aTGGATCTGCTGCGTAAGCTCAACTACA‑CCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAG‑ATGATCCTGAC‑ACTGGCACCGGAAACCAACGGTCAGGTA‑GCCGTGAAAGCCTGGG‑CTGCCCTGAGC‑GAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGcc                                        >  1:267113/1‑239 (MQ=255)

                                                                                                                                                                                                      aTGGATCTGCTGCGTAAGCTCAACTACA‑CCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAG‑ATGATCCTGAC‑ACTGGCACCGGAAACCAACGGTCAGGTA‑GCCGTGAAAGCCTGGG‑CTGCCCTGAGC‑GAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGcc                                        >  1:1001049/1‑239 (MQ=255)

                                                                                                                                                                                                           tCTGCTGCGTAAGCTCAACTACA‑CCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAG‑ATGATCCTGAC‑ACTGGCACCGGAAACCAACGGTCAGGTA‑GCCGTGAAAGCCTGGG‑CTGCCCTGAGC‑GAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCgcgc                                    <  1:794299/238‑1 (MQ=255)

                                                                                                                                                                                                               ctgcGTAAGCTCAACTACA‑CCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAG‑ATGATCCTGAC‑ACTGGCACCGGAAACCAACGGTCAGGTA‑GCCGTGAAAGCCTGGG‑CTGCCCTGAGC‑GAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAAt                               >  2:878143/1‑239 (MQ=255)

                                                                                                                                                                                                               ctgcGTAAGCTCAACTACA‑CCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAG‑ATGATCCTGAC‑ACTGGCACCGGAAACCAACGGTCAGGTA‑GCCGTGAAAGCCTGGG‑CTGCCCTGAGC‑GAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAAt                               >  2:1166584/1‑239 (MQ=255)

                                                                                                                                                                                                                tgcGTAAGCTCAACTACA‑CCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAG‑ATGATCCTGAC‑ACTGGCACCGGAAACCAACGGTCAGGTA‑GCCGTGAAAGCCTGGG‑CTGCCCTGAGC‑GAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAAt                               <  1:899405/238‑1 (MQ=255)

                                                                                                                                                                                                                 gcGTAAGCTCAACTACA‑CCAAAGCGGAAGGTCCGGCGAAAGGAGAGAAGAAGAAGAAAAAAGAAAAGGAAGAGGAAGAG‑AAGAGAGTGAA‑ACTGGCACCGGAAACCAACGGTCAGGTA‑GGAGGGAAAGAAGGGG‑ATGGAAGGAGA‑GAAAAAAAAGGGAGAGACCAGACGCAACCGGCGCCGAATAAAGAAGACGAGAAGATCCGCTTCCGAGATAGTCAGGCAAAGCCGCGCCAAATTa                             >  2:925364/1‑239 (MQ=255)

                                                                                                                                                                                                                  cGTAAGCTCACCTACG‑CCAAAGCGGAAGGGCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAG‑ATGATCCTGAC‑ACTGGCACCGGAAACCAACGGTCAGGTA‑GCCGTGAAAGCCTGGG‑CTGCCCTGAGC‑GAATTTACCGGTCGTGACCATACGCATCTGgcg                                                                                          <  2:209453/177‑1 (MQ=255)

                                                                                                                                                                                                                    taggCTCAACTACA‑CCAAAGCGGAAGGTCCGGCGAAAGGCCAGCGGATGTTGAACACGCAAATTGAGGGGGCAGAG‑ATGATCCGGAC‑ACTGGCCCCGGAAACAACCGGTCAGGAG‑GCCGTAAAAGCCGGGG‑CGGCCCTGAGG‑GAATTTACCGGTGGGGACCATAGGCATCTGGCGCTGATTAAAGAGGACGAGAAGATCCGCTTCCGCGATATTCAGGCACACCCCCGAAAAATTAtc                           >  2:194226/4‑238 (MQ=255)

                                                                                                                                                                                                                    tAAGCTCAACTACA‑CCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAG‑ATGATCCTGAC‑ACTGGCACCGGAAACCAACGGTCAGGTA‑GCCGTGAAAGCCTGGG‑CTGCCCTGAGC‑GAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTAtc                           >  2:156257/1‑238 (MQ=255)

                                                                                                                                                                                                                    tAAGCTCAACTACA‑CCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAG‑ATGATCCTGAC‑ACTGGCACCGGAAACCAACGGTCAGGTA‑GCCGTGAAAGCCTGGG‑CTGCCCTGAGC‑GAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTAtc                           >  1:407029/1‑238 (MQ=255)

                                                                                                                                                                                                                    tAAGCTCAACTACA‑CCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAG‑ATGATCCTGAC‑ACTGGCACCGGAAACCAACGGTCAGGTA‑GCCGTGAAAGCCTGGG‑CTGCCCTGAGC‑GAATTTACCGGTCGTGACCATACGCATCTGGCCCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGCCACAGCCGCGCAAAATTAtc                           >  2:822045/1‑238 (MQ=255)

                                                                                                                                                                                                                    tAAGCTCAACTACA‑CCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAG‑ATGATCCTGAC‑ACTGGCACCGGAAACCAACGGTCAGGTA‑GCCGTGAAAGCCTGGG‑CTGCCCTGAGC‑GAATTTACCGGTCGTGACCATACGCATCTGGAGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCCCAGCCGCGCACAATTAtc                           >  2:596008/1‑238 (MQ=255)

                                                                                                                                                                                                                     aaGCTCAACTACA‑CCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAG‑ATGATCCTGAC‑ACTGGCACCGGAAACCAACGGTCAGGTA‑GCCGTGAAAGCCTGGG‑CTGCCCTGAGC‑GAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTAtct                          <  1:152365/238‑1 (MQ=255)

                                                                                                                                                                                                                          cAACTACA‑CCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAG‑ATGATCCTGAC‑ACTGGCACCGGAAACCAACGGTCAGGTA‑GCCGTGAAAGCCTGGG‑CTGCCCTGAGC‑GAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGcc                    <  1:82738/239‑1 (MQ=255)

                                                                                                                                                                                                                            aCTACA‑CCAAAGCGGAAGGTCCGGTGAAGGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAG‑ATGATCCTGAC‑ACTGGCACCGGAAACCAACGGTCAGGTA‑GCCGTGAAAGCCTGGG‑CTGCCCTGAGC‑GAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCg                  <  1:1093540/239‑1 (MQ=255)

                                                                                                                                                                                                                            aCTACA‑CCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAG‑ATGATCCTGAC‑ACTGGCACCGGAAACCAACGGTCAGGTA‑GCCGTGAAAGCCTGGG‑CTGCCCTGAGC‑GAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAaag                                                                                >  1:209453/1‑177 (MQ=255)

                                                                                                                                                                                                                            aCTACA‑CCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAG‑ATGATCCTGAC‑ACTGGCACCGGAAACCAACGGTCAGGTA‑GCCGTGAAAGCCTGGG‑CTGCCCTGAGC‑GAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCg                  >  1:165449/1‑239 (MQ=255)

                                                                                                                                                                                                                              tACA‑CCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAG‑ATGATCCTGAC‑ACTGGCACCGGAAACCAACGGTCAGGTA‑GCCGTGAAAGCCTGGG‑CTGCCCTGAGC‑GAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGAc                <  1:599325/239‑1 (MQ=255)

                                                                                                                                                                                                                                 a‑cCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAG‑ATGATCCTGAC‑ACTGGCACCGGAAACCAACGGTCAGGTA‑GCCGTGAAAGCCTGGG‑CTGCCCTGAGC‑GAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACctg             >  2:405979/1‑239 (MQ=255)

                                                                                                                                                                                                                                    taaaGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAG‑ATGATCCTGAC‑ACTGGCACCGGAAACCAACGGTCAGGTA‑GCCGTGAAAGCCTGGG‑CTGCCCTGATC‑GAATTTACCGGTCTTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACctggt           <  1:880810/238‑1 (MQ=255)

                                                                                                                                                                                                                                    cAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAG‑ATGATCCTGAC‑ACTGGCACCGGAAACCAACGGTCAGGTA‑GCCGTGAAAGCCTGGG‑CTGCCCTGAGC‑GAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACctggt           <  1:590893/239‑1 (MQ=255)

                                                                                                                                                                                                                                      acgCGGAAGGTCCCGCGAAAGGCCAGCCGATGCTGAACACCACAATTGATGCGGCAGAG‑ATGAACCTGAC‑ACTGGCACCGGAAACCAACGGTCAGGTA‑GCCGTGAAAGCCTGGG‑CTGCCCTGAGC‑GAATTTACCGGTCGAGTCCTTACGAATCTGGCTCTGAATAATGTAGTCGAGAAGATTCGCGTCCGCGACATTCAGGCACAGACGCGCAAAATTTGCCCCAGCCCCACctgg            >  2:529072/3‑236 (MQ=255)

                                                                                                                                                                                                                                      aaGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAg‑atgat                                                                                                                                                                                            <  1:772232/64‑1 (MQ=255)

                                                                                                                                                                                                                                      aaGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAg‑atgat                                                                                                                                                                                            >  2:772232/1‑64 (MQ=255)

                                                                                                                                                                                                                                      aaGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAG‑ATGATCCTGAC‑ACTGGCACCGGAAACCAACGGTCAGGTA‑GCCGTGAAAGCCTGGG‑CTGCCCTGAGC‑GAATTTACCGGTCGTGCCCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGTCACAGCCGCGCAAAATTATCTCCAGCCCGCCctgg            >  1:674592/1‑236 (MQ=255)

                                                                                                                                                                                                                                      aaGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAG‑ATGATCCTGAC‑ACTGGCACCGGAAACCAACGGTCAGGTA‑GCCGTGAAAGCCTGGG‑CTGCCCTGAGC‑GAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACctgg            >  1:332244/1‑236 (MQ=255)

                                                                                                                                                                                                                                      aaGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAG‑ATGATCCTGAC‑ACTGGCACCGGAAACCAACGGTCAGGTA‑GCCGTGAAAGCCTGGG‑CTGCCCTGAGC‑GAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACctgg            >  1:546243/1‑236 (MQ=255)

                                                                                                                                                                                                                                      aaGCGGAAGGTCCGGCGAAACGCCAGCCGATGCTGCACACCGCAATTGATGCGGCAGAG‑ATGATCCTGAC‑ACAGGCACCGGAAACCAACGGTCAGGGA‑GCCGTGAAAGCCTGGG‑CTGCCCTGAGC‑GAATGTACCGGTCGTGCCCATACGCATGTCGCGCTGAATAAAGAAGACGAGAAGATCCCCTTCACCGATCTTCAGGCACAGCCGCGCAACATTATTTCAGGCCCGACctgg            >  2:1166565/1‑236 (MQ=255)

                                                                                                                                                                                                                                          ggAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAG‑ATGATCCTGAC‑ACTGGCACCGGAAACCAACGGTCAGGTA‑GCCGTGAAAGCCTGGG‑CTGCCCTGAGC‑GAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACctggtctgg       <  1:1166584/237‑1 (MQ=255)

                                                                                                                                                                                                                                          ggAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGACGCGGCAGAG‑ATGATCCTGAC‑ACTGGCACCGGAATCCAACGGTCAGGTA‑GCCGTGAAAGCCTGGG‑CTGCCCTGAGC‑GAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCtggtctggtc     <  1:421599/239‑1 (MQ=255)

                                                                                                                                                                                                                                            aaGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAG‑ATGATCCTGAC‑ACTGGCACCGGAAACCAACGGTCAGGTA‑GCCGTGAAAGCCTGGG‑CTGCCCTGAGC‑GAATTTACCGGTCGTGACCATACGCATCTGgcg                                                                                          <  1:332754/152‑1 (MQ=255)

                                                                                                                                                                                                                                            aaGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAG‑ATGATCCTGAC‑ACTGGCACCGGAAACCAACGGTCAGGTA‑GCCGTGAAAGCCTGGG‑CTGCCCTGAGC‑GAATTTACCGGTCGTGACCATACGCATCTGgcg                                                                                          >  2:332754/1‑152 (MQ=255)

                                                                                                                                                                                                                                            aaGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAG‑ATGATCCTGAC‑ACTGGCACCGGAAACCAACGGTCAGGTA‑GCCGTGAAAGCCTGGG‑CTGCCCTGAGC‑GAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGgtctggtctg   >  1:986516/1‑239 (MQ=255)

                                                                                                                                                                                                                                             aGGTCCGGCGAAAGGCCAGCCGAGGCTGAACACCGCAATTGATGCGGCAGAG‑ATGATCCTGAC‑ACTGGCACCGGAAACCAACGGTCAGGTA‑GCCGTGAAAGCCTGGG‑CTGCCCTGAGC‑GAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGtctggtctgg  <  1:64069/239‑1 (MQ=255)

TGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTG‑CCTGGAACACCCAGAGCG‑AGATGGATCTGCTGCGTAAGCTCAACTACA‑CCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAG‑ATGATCCTGAC‑ACTGGCACCGGAAACCAACGGTCAGGTA‑GCCGTGAAAGCCTGGG‑CTGCCCTGAGC‑GAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGG  >  NC_000913/1282440‑1282912

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A