Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F17 I0 R1	115	121.7	2441348	96.4%	2353459	231.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,904,285	C→A	11.6%	A438S (GCC→TCC)	bglF ←	fused beta‑glucoside‑specific PTS enzymes: IIA component/IIB component/IIC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,904,285	0	C	A	11.6%	394.1 / 2.8	126	A438S (GCC→TCC)	bglF	fused beta‑glucoside‑specific PTS enzymes: IIA component/IIB component/IIC component
Reads supporting (aligned to +/- strand): ref base C (89/19); new base A (2/15); total (91/35)
Fisher's exact test for biased strand distribution p-value = 1.95e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

Only 100 of 127 total aligned reads displayed.

CGCACTTCACCAACCGAGGGCAGAATGGCAATACCTTTACCCAACAGGCCACTGGCAAACGTGGTATCAGCGACGTGAATGAGCGGCACAATCTCT‑CCCGTCATCGGTGAACAGATACCGCCCTGCTCAGGTGGTGT‑AATAACC‑TCTGGTGTTTTCTCTT‑GCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAA  >  NC_000913/3904047‑3904516

       cACCAACCGAGGGCAGAATGGCAATACCTTTACCCAACAGGCCACTGGCAAACGTGGTATCAGCGACGTGAATGAGCGGCACAATCTCT‑CCCGTCATCGGTGAACAGATACCGCCCTGCTCAGGTGGTGT‑AATAACC‑TCTGGTGTTTTCTCTT‑GCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGGCAATGac                                                                                                                                                                                                                                    >  1:719642/1‑238 (MQ=255)

             ccGAGGGCAGAATGGCAATACCTTTACCCAACAGGCCACTGGCAAACGTGGTATCAGCGACGTGAATGAGCGGCACAATCTCT‑CCCGTCATCGGTGAACAGATACCGCCCTGCTCAGGTGGTGT‑AATAACC‑TCTGGTGTTTTCTCTT‑GCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGGCAATGACA‑CCGCCa                                                                                                                                                                                                                            >  1:1020636/1‑239 (MQ=255)

               gAGGGCAGAATGGCAATACCTTTACCCAACAGGCCACTGGCAAACGTGGTATCAGCGACGTGAATGAGCGGCACAATCTCT‑CCCGTCATCGGTGAACAGATACCGCCCTGCTCAGCTGGTGT‑AATAACC‑TCTGGTGTTTTCTCTT‑GCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGAACCGACAAATGCGAAACCGATGGCAATGACA‑CGACCAAt                                                                                                                                                                                                                          >  2:339864/1‑239 (MQ=255)

                    cagagggacAATACCGCTACCCAACAGGCCACTGGCAAACGTGCTATCCGCGACGTGAAGGAGCGCCACCATCTCC‑CCCTTCTTCGGTGAACAGATCCCGCCCCCCTCGGGTGGTTT‑AATACCC‑TCTGGTGTTTTCTCTT‑GCGGGGCCCCCTGCGCTGCATGCCGTTTACCGGTGATAAAATAAAGCATCCCCGTACCGACAAATCCGCAACCGATGGCAATGACA‑CCGCCAATAACg                                                                                                                                                                                                                      <  2:126622/230‑1 (MQ=255)

                                  ctttaccagtcagcacACTGCCAAACGTGGTATCAGCGCAGTGAATGGGCGGCGCAATCTCT‑CCAGTCTGCGGTGAACAGACACCGCCGTGTGCGGGTGGTGT‑AATACCC‑CCTGGTTTTTTCCCTG‑GCGGGTCACCCTGCGCTGGCTGACGTTAACGGGTGATAAAAAAAAGCACCACAGTCCCGACAAAGGCGCAAACGTTGGTAACCACA‑CCGCCAATAACGCTGGCCCAGACGGTg                                                                                                                                                                                                       <  2:1161256/224‑1 (MQ=255)

                                   tttACCCAACAGGCCACTGGCAAACGTGGTATCAGCGACGTGAATGAGCGGCACAATCTCT‑CCCGTCATCGGTGAACAGATACCGCCCTGCTCAGGTGGTGT‑AATAACC‑TCTGGTGTTTTCTCTT‑GCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGAGGGGGa                                                                                                                                                                                                      >  1:1108950/1‑239 (MQ=255)

                                     tACCCAACAGGCCACTGGCAAACGTGGTATCAGCGACGTGAATGAGCGGCACAATCTCT‑CCCGTAATCGGTGAACAAATACCGCCCTGCTCAGGCAGGGT‑AATAACC‑TCTGGTGTTTTCTCTT‑GCGGGGCACCCTGGGCTGGCTGACGTTTAGCGGTAAGGAAATGAAGCATCACCGTACCGACAAATGCGCAACGAATGGAAATGACA‑CCGCCAATAACGCTGGCCCAGACGGGGaat                                                                                                                                                                                                    >  2:743141/1‑238 (MQ=255)

                                          aaCAGGCCACTGGCAAACGTGGTATCAGCGACGTGAATGAGCGGCACAATCTCT‑CCCGTCATCGGTGAACAGATACCGCCCTGCTCAGGTGGTGT‑AATAACC‑TCTGGTGTTTTCTCTT‑GCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAAt                                                                                                                                                                                               >  1:1170614/1‑239 (MQ=255)

                                          aaCAGGCCACTGGCAAACGTGGTATCAGCGACGTGAATGAGCGGCACAATCTCT‑CCCGTCATCGGTGAACAGATACCGCCCTGCTCAGGTGGTGT‑AATAACC‑TCTGGTGTTTTCTCTT‑GCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAAt                                                                                                                                                                                               >  1:960856/1‑239 (MQ=255)

                                           aCAGGCCACTGGCAAACGTGGTATCAGCGACGTGAATGAGCGGCACAATCTCT‑CCCGTCATCGGTGAACAGATACCGCCCTGCTCAGGTGGTGT‑AATAACC‑TCTGGTGTTTTCTCTT‑GCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGGCAATGACA‑CCGCCACTAACGCTGGCCCAGACGGTGAAATCAAt                                                                                                                                                                                               >  1:645280/1‑238 (MQ=255)

                                            cAGGCCACTGGCAAACGTGGTATCAGCGACGTGAATGAGCGGCACAATCTCT‑CCCGTCATCGGTGAACAGATACCGCCCTGCTCAGGTGGTGT‑AATAACC‑TCTGGTGTTTTCTCTT‑GCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAAtt                                                                                                                                                                                              >  1:1058404/1‑238 (MQ=255)

                                            cAGGCCACTGGCAAACGTGGTATCAGCGACGTGAATGAGCGGCACAATCTCT‑CCCGTCATCGGTGAACAGATACCGCCCTGCTCAGGTGGTGT‑AATAACC‑TCTGGTGTTTTCTCTT‑GCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAAtt                                                                                                                                                                                              >  1:689981/1‑238 (MQ=255)

                                            cAGGCCACTGGCAAACGTGGTATCAGCGACGTGAATGAGCGGCACAATCTCT‑CCCGTCATCGGTGAACAGATACCGCCCTGCTCAGGTGGTGT‑AATAACC‑TCTGGTGTTTTCTCTT‑GCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAAtt                                                                                                                                                                                              >  1:1195869/1‑238 (MQ=255)

                                            cAGGCCACTGGCAAACGTGGTATCAGCGACGTGAATGAGCGGCACAATCTCT‑CCCGTCATCGGTGAACAGATACCGCCCTGCTCAGGTGGTGT‑AATAACC‑TCTGGTGTTTTCTCTT‑GCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAAtt                                                                                                                                                                                              >  2:319319/1‑238 (MQ=255)

                                            cAGGCCACTGGCAAACGTGGTATCAGCGACGTGAATGAGCGGCACAATCTCT‑CCCGTCATCGGTGAACAGATACCGCCCTGCTCAGGTGGTGT‑AATAACC‑TCTGGTGTTTTCTCTT‑GCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAAtt                                                                                                                                                                                              >  1:354460/1‑238 (MQ=255)

                                             aGGCCACTGGCAAACGTGGTATCAGCGACGTGAATGAGCGGCACAATCTCT‑CCCGTCATCGGTGAACAGATACCGCCCTGCTCAGGTGGTGT‑AATAACC‑TCTGGTGTTTTCTCTT‑GCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGGGAAAGCAATTc                                                                                                                                                                                             >  2:544454/1‑238 (MQ=255)

                                               gCCACTGGCAAACGTGGTATCAGCGACGTGAATGAGCGGCACAATCTCT‑CCCGTCATCGGTGAACAGATACCGCCCTGCTCAGGTGGTGT‑AATAACC‑TCTGGTGTTTTCTCTT‑GCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCg                                                                                                                                                                                          >  1:100558/1‑239 (MQ=255)

                                               gCCACTGGCAAACGTGGTATCAGCGACGTGAATGAGCGGCACAATCTCT‑CCCGTCATCGGTGAACAGATACCGCCCTGCTCAGGTGGTGT‑AATAACC‑TCTGGTGTTTTCTCTT‑GCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGGCAATGACA‑CCGCCAATAACGCAGGCCCAGACGGTGAAATCAATTCCCg                                                                                                                                                                                          >  1:458815/1‑239 (MQ=255)

                                               gCCACTGGCAAACGTGGTATCAGCGACGTGAATGAGCGGCACAATCTCT‑CCCGTCATCGGTAAACAGATACCGCCCTGCTCAGGTGGTGT‑AATAACC‑TCTGGTGTTTTCTCTT‑GCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCg                                                                                                                                                                                          >  1:674386/1‑239 (MQ=255)

                                               gCCACTGGCAAACGTGGCATCAGCGACGTGAATGAGCGGCACAATCTCT‑CCCGTCATCGGTGAACAGATACCGCCCTGCTCAGGTGGTGT‑AATAACC‑TCTGGTGTTTTCTCTT‑GCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGCCAAATGCGCAACCGATGGCAATGACA‑CCGCCAATAACACTGGCCGAGACGGTGAAATCAATTCCCg                                                                                                                                                                                          >  1:329559/1‑239 (MQ=255)

                                                  aCTGCCATACGTGGTATCAGCGCCGTGAAGGGGCGGCACAATCTCT‑CCCGTCGTCGGGTAACAAATCCCGCCCTGCCCGGGGGGTGT‑AATACCC‑TCGGGTTTTTTTTCTT‑GCGGGGCCCCTTGCGCGGGCTGACTTTTAGCGGGGAAAAAAGAAAGCATCACCGTACCGAAAAAGGCGCACCCGATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTg                                                                                                                                                                                       <  2:785966/239‑1 (MQ=255)

                                                    tGGCAAACGTGGTATCAGCGACGTGAATGAGCGGCACAATCTCT‑CCCGTCATCGGTGAACAGATACCGCCCTGCTCAGGTGGTGT‑AATAACC‑TCTGGTGTTTTCTCTT‑GCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGGCAATGACA‑CCGCCAATAACGCTGGCCCCGACGGTGAAATCAATACCCATTGAc                                                                                                                                                                                     >  2:390910/1‑239 (MQ=255)

                                                      gCAAACGTGGTATCAGCGACGTGAATGAGCGGCACAATCTCT‑CCCGTCATCGGTGAACAGATACCGCCCTGCTCAGGTGGTGT‑AATAACC‑TCTGGTGTTTTCTCTT‑GCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACgg                                                                                                                                                                                   >  1:394103/1‑239 (MQ=255)

                                                                tATCAGCGACGTGAATGAGCGGCACAATCTCT‑CCCGTCATCGGTGAACAGATACCGCCCTGCTCAGGTGGTGT‑AATAACC‑TCTGGTGTTTTCTCTT‑GCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCCCCGTACCGACAAATGCGCAACCGATGGCAATGACA‑ACGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGc                                                                                                                                                                         >  2:1067865/1‑239 (MQ=255)

                                                                   cggCGACGTAAGTGGGGGGCAAATCCTCC‑CCGGTCATCTGTAAACACAGCCCGCCCGGCACGGGGGGGGA‑AAAAACT‑TCGGGTATTTTCTCTT‑GCGGGGCACCCTGCGGGGACGACCGTTGAGCGGTGAAGAAATAAAGCATCCCCGTCCAGAAAAACGCGAAACGAAAAGAAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATg                                                                                                                                                                      <  2:1173834/237‑1 (MQ=255)

                                                                   aagggggttGAATGGGCGGCACAATCCCC‑CCCTTCAGCGGTGAACAGTGCCCGCCCTGCCGAGGTGTGGT‑ATTACCC‑GTTGGTGTTTTCTTTG‑GCGGGGCCCCCGGCGCGGGCTGACGTTAGGGGGGAATAAAATGAACCATCCCCGTACCGACAAATGCGCAACCGAGGGAAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATg                                                                                                                                                                      <  2:960856/231‑1 (MQ=255)

                                                                          gTGAGTGCGCGGCA‑AATCCCTCCCCTTCATGGGTGAACATTTCCCGCCGTGCTCAGGT‑GTGTAAAGACCC‑TCGGGGTTTTTCCTTG‑GCGGGCCCCCCTCCCCGGTATGCCGTTTGGGGGTGACAAAATAACCCATCACCGTCCCAAAAAATGCGAAACAGAGGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGa                                                                                                                                                               <  2:576034/239‑1 (MQ=255)

                                                                          gTGAATGAGCGGCACAATCTCT‑CCCGTCATCGGTGAACAGATACCGCCCTGCTCAGGTGGTGT‑AATAACC‑TCTGGTGTTTTCTCTT‑GCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGa                                                                                                                                                               >  1:302135/1‑239 (MQ=255)

                                                                                     ggctatattaccccattCATGGGTGTACAGATCCCCCTCTGCCGGGGTTGTTT‑AATAACC‑T‑TGGGTTTGTCTTTTGGCGGCCCCCCCGGGGCGGGAGGTCGTTTAGCGGTGAAAAAAAGAAGCACACCCGCACCGACAAAGGCCCAACATATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTgg                                                                                                                                                     <  2:1077472/223‑1 (MQ=255)

                                                                                     gccaactctct‑cCCGTCATCGGTGAACAGATCCCGCCCTGCTCAGGTGGTTT‑AATACCC‑TCTGGTGTTTTCTCTT‑GCGGGGCACCCTGCGCTGGCGGACGTTTAGCGGTGATGAAATGAAGCATCCCCGTACCGACAAATGCGCACCCGATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTgg                                                                                                                                                     <  1:540544/232‑1 (MQ=255)

                                                                                           tctct‑cCCGTCATCGGTGAACAGATACCGCCCTGCTCAGGCGGTGT‑AATAACC‑TCTGGTGTTTTCTCTT‑GCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGCGATGAAATGAAGCATCACCGCACCGACAAATGCGCAACCGATGGCAATGACA‑CCGCCACTAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGGTTCCATGACGGTGAACATACTTGGCCACCCa                                                                                                                                              >  2:704898/1‑239 (MQ=255)

                                                                                              ct‑cCCGTCAGGGGTGAACAGATCCCGCCTTGCTCAGGTGGTGT‑AATACCC‑TCTGGTGTTTTCTCTT‑GCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATAAAATGAAGCATCCCCGTACCGACAAATGCGCAACCGAGGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAg                                                                                                                                           <  1:780296/239‑1 (MQ=255)

                                                                                                       aTCGGTGAACAGATACCGCCCTGCTCAGGTGGTGT‑AATAACC‑TCTGGTGTTTTCTCTT‑GCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGCAGGTGAAAATACTTGGCAAACCAAAGGAGTAg                                                                                                                                     >  2:867781/1‑237 (MQ=255)

                                                                                                       aTCGGTGAACAGATACCGCCCTGCTCAGGTGGTGT‑AATAACC‑TCTGGTGTTTTCTCTT‑GCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGACGGTGAAAATACTTGGCAAACCAAAGGAGTAg                                                                                                                                     >  1:154097/1‑237 (MQ=255)

                                                                                                       aTCGGTGAACAGATACCGCCCTGCTCAGGTGGTGT‑AATAACC‑TCTGGTGTTTTCTCTT‑GCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAg                                                                                                                                     >  1:544721/1‑237 (MQ=255)

                                                                                                       aTCGGTGAACAGATACCGCCCTGCTCAGGTGGTGT‑AATAACC‑TCTGGTGTTTTCTCTT‑GCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAg                                                                                                                                     >  1:1178651/1‑237 (MQ=255)

                                                                                                       aTCGGTGAACAGATACCGCCCTGCTCAGGTGGTGT‑AATAACC‑TCTGGTGTTTTCTCTT‑GCGGGGCACCCTGCGCTGGCTGACGCTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGGCAATGACA‑CCGCCAATAACGCTGGCCAAGCCGGTGAAATCAATTCCCGTTGACGGGACGGTTTGCATGAAGGTGAAAATACTTGCCAACCCAAAGGAGAAg                                                                                                                                     >  2:826932/1‑237 (MQ=255)

                                                                                                           gTGAACAGATACCGCCCTGCTCAGGTGGTGT‑AATAACC‑TCTGGTGTTTTCTCTT‑GCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTc                                                                                                                               >  2:1216215/1‑239 (MQ=255)

                                                                                                                    tcccGCCCGGCTCAGGTGGTGT‑AATAACC‑TGTGGTGTTTTCTCTT‑GCGGGGCCCCCGGCGCGGGCTGACGTTGAGCGGTGAGAAAAGGAAGCATCACCGTACCGACAAAGGCGAAACCGAGGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTgcgc                                                                                                                       <  1:29540/236‑1 (MQ=255)

                                                                                                                      ccctctggCTCAGGTGGTGT‑‑ATAACCTTGTGTTGTTTTCTCTT‑CGGGGGCCCCCTCCGCGGACGGTCGTTTAGCGGTGAGGAAAGGACACATCCCCTCCCAGCCAAATGCGAAACCAATGGAAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTa                                                                                                                    <  2:302135/232‑1 (MQ=255)

                                                                                                                       ccccctcgtcggggggtgT‑AATAACC‑TCTGGTGTTTTCTCTT‑GCGGGGCCCCCTGCGCGGGTTGACGTTTAGCGGTGAAAAAAAGAAGCATAACCGTACCGAAAAAGGCGAAAACGATGGCAATGATT‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTa                                                                                                                    <  2:419601/224‑1 (MQ=255)

                                                                                                                        gCCCTGCTCAGGTGGTGT‑AATAACC‑TCTGGTGTTTTCTCTT‑GCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAg                                                                                                                   >  1:825928/1‑238 (MQ=255)

                                                                                                                        gCCCTGCTCAGGTGGTGT‑AATAACC‑TCTGGTGTTTTCTCTT‑GCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAg                                                                                                                   >  1:329810/1‑238 (MQ=255)

                                                                                                                        gCCCTGCTCAGGTGGTGT‑AATAACC‑TCTGGTGTTTTCTCTT‑GCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTACCGTTGACGGGATGGTTTGCATGAAGGGGAAAATAATTGGCAAACCAAAGGAGTAGGCTTTCGTTTGCGCGGAg                                                                                                                   >  2:902517/1‑238 (MQ=255)

                                                                                                                        gCCCTGCTCAGGTGGTGT‑AATAACC‑TCTGGTGTTTTCTCTT‑GCGGGGAACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGCCGGGATGGTTTGCATGAAGGGGAAAATAATTGGAAAACCAAAGGAGGAGACTTTCGTTTGCGCGTAg                                                                                                                   >  2:392961/1‑238 (MQ=255)

                                                                                                                         cccTGCTCAGTTGGTAT‑AATAACC‑TCTGGTGTTTTCTCGT‑GCGGGGCCCCCTGCGCTGGCGGACTTTTAGCGGTGATGAAATGAAGCACCACCGTACCGAAAAATGCGCAACCGATGGAAAGGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGcc                                                                                                                 <  1:701992/239‑1 (MQ=255)

                                                                                                                          ccTGCTCAGGTGGTGT‑AATAACC‑TCTGGTGTTTTCTCTT‑GCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGCCTTTCGTTGGCGCGTAGcaa                                                                                                                >  2:1164775/1‑237 (MQ=255)

                                                                                                                           cTGCTCAGGTGGTGT‑AATAACC‑TCTGGTGTTTTCTCTT‑GCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCaa                                                                                                               >  1:350875/1‑239 (MQ=255)

                                                                                                                             gCTCAGGTGGTGT‑CATAACA‑TCTGGTGTTTTCTCTT‑GCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGAGAAATCAATTCACGCTGACGGGACGGTTTGCATGAAGGTGACAATACCTGGCAAACCAAAGGAGTAGGCTTTCGTTGGCGCTAAGCCaata                                                                                                             >  2:362889/1‑239 (MQ=255)

                                                                                                                              cTCAGGTGGTGT‑AATAACC‑TCTGGTGTTTTCTCTT‑GCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCAaataa                                                                                                            >  1:1014377/1‑239 (MQ=255)

                                                                                                                                      gtgt‑AATAACC‑TCTGGTGTTTTCTCTT‑GCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGAGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGc                                                                                                     >  1:637808/1‑238 (MQ=255)

                                                                                                                                        gt‑AATAACC‑TCTGGTGTTTTCTCTT‑GCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGTTGAAATGAAGCATCACCGTACCGGCAAATGCGCAACCGATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGACATCAATTCCCGTTGTCGGGAAGGGTTGCATGAAGGTGAAGATACTTGACAAAACAAAGGCGTAAACTTTTGTTTGCGCGAAGCGAATAATGGAGGacc                                                                                                   >  2:863960/1‑238 (MQ=255)

                                                                                                                                        gt‑AATAACC‑TCTGGTGTTTTCTCTT‑GCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCTTGAAGGTGAAAATACTTGGCAACCCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGccc                                                                                                   >  2:845439/1‑238 (MQ=255)

                                                                                                                                        gt‑AATAACC‑TCTGGTGTTTTCTCTT‑GCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAGATACTTAGCAACCCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGccc                                                                                                   >  2:315024/1‑238 (MQ=255)

                                                                                                                                        gt‑AATAACC‑TCTGGTGTTTTCTCTT‑GCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAACCCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGccc                                                                                                   >  2:500238/1‑238 (MQ=255)

                                                                                                                                        gt‑AATAACC‑TCTGGTGTTTTCTCTT‑GCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGAAAAATGCGCAACCGATGGCAATGACA‑CCGCCAATAACGCTGGTCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTa                                                                                                                    <  1:896544/221‑1 (MQ=255)

                                                                                                                                              aaCC‑TCTGGTGTTTTCTCTT‑GCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGGTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCaaa                                                                                              >  1:634846/1‑238 (MQ=255)

                                                                                                                                               aCC‑TCTGGTGTTTTCTCTT‑GCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGAAGACTTTCGTTTGCGCGTAGCCAATAATGGGGGCCCCCAAAg                                                                                             >  2:920743/1‑238 (MQ=255)

                                                                                                                                                   tctGGTGTTTTCTCTT‑GCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGccac                                                                                         >  1:68117/1‑237 (MQ=255)

                                                                                                                                                   tctGGTGTTTTCTCTT‑GCGGAGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGcccc                                                                                         >  2:1019675/1‑239 (MQ=255)

                                                                                                                                                    ctGGTGTTTTCTCTT‑GCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGcccc                                                                                         >  2:88089/1‑238 (MQ=255)

                                                                                                                                                    ctGGTGTTTTCTCTT‑GCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGGGATGAAATGAAGCATCACCGTCCCGACAAATGCGCAACCGATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGCGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAGTAGTGGCCCCCAAAGccgc                                                                                         >  2:587621/1‑236 (MQ=255)

                                                                                                                                                     tGGTGTTTTCTCTT‑GCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAAtg                                                                                                          >  1:1111365/1‑220 (MQ=255)

                                                                                                                                                     tGGTGTTTTCTCTT‑GCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAACGcccc                                                                                         >  1:1121218/1‑237 (MQ=255)

                                                                                                                                                     tGGTGTTTTCTCTT‑GCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGcacc                                                                                         >  2:916013/1‑237 (MQ=255)

                                                                                                                                                     tGGAGTTTTCTCTT‑GCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAATTGAAGCATCACCGTACCGACAAATTCGCAACAGATAGCAATGACA‑CAGCAAATAAAGCGGGCCCCGACGGTCAAATCAATTCCCGTCGACCGGTTGGTTTGCATGATGGTGACAATACTTGGCACACCAAAGGGGTAGACTTTCGTTGGCACGTTGCCAATGATGCTCGCCATCAAAGCCacac                                                                                       >  2:652297/1‑239 (MQ=255)

                                                                                                                                                     tGGAGTTTTCTCTT‑GCAGCGCCGCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGGGAAAATACTTGTCCAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGATGGCCCCCAAAGcccc                                                                                         >  2:453468/1‑237 (MQ=255)

                                                                                                                                                     gggTGTTTCCCCTT‑GCGGGCCCCCTCGCGAGGGCTGACGTTGAGCGGTGATAAAATAAAGCACCACCGTACAGACAAATGGGCAACCGATGGAAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCAc                                                                                       <  1:826932/238‑1 (MQ=255)

                                                                                                                                                      ggTGTTTTCTCTT‑GCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGcccc                                                                                         >  2:986151/1‑236 (MQ=255)

                                                                                                                                                         gtTTTCTCTT‑GCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGAt                                                                                   >  1:471794/1‑239 (MQ=255)

                                                                                                                                                          ttatctcTG‑GCGGGGCCCCGCGCGTGGTCTAACGTTTAGCGTGTAAGAAATGAACCACCACCGTACCGCAAAAGGCCAACCCGTTGGAAATAACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATa                                                                                  <  1:515602/236‑1 (MQ=255)

                                                                                                                                                            ttCTCTT‑GCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACTGACAAATGCGCAACCGATGGCAATGACA‑CCGCCAATAACGCTGGCCAAGACGGTGAAATCAATTCCCGTTGAGGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGGAGACTTTCGTTTGCGCGAAGCCAATAATGGTGGCCCCCGAAGACCCACTGAGACa                                                                                >  2:231607/1‑239 (MQ=255)

                                                                                                                                                            ttCTCTT‑GCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACa                                                                                >  1:646286/1‑239 (MQ=255)

                                                                                                                                                            ttCTCTT‑GCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACa                                                                                >  1:521692/1‑239 (MQ=255)

                                                                                                                                                            ttCTCTT‑GCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGGGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACa                                                                                >  1:472540/1‑239 (MQ=255)

                                                                                                                                                            ttCTCTT‑GCGGGGCACCATGCGCTGGCTGACGTTTAGCGGTGATGAACTGAAGCATCACCGTACCGACAAATGCGCAACCGATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGCGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTTCGCGTAGCCACTAATGGTGG‑CCCCAAAGCCCCAATGATACAg                                                                               >  2:1194409/1‑239 (MQ=255)

                                                                                                                                                             tcctttggCGGGGCCCCCTGCTGTGTCTGCAGGTTAGTGGTAATAAATCGCAGCACCGCCACCCCGACAATTCCCCAGCATATGCCATTGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACa                                                                                <  2:719642/232‑1 (MQ=255)

                                                                                                                                                                       gggCACCCTGCGCTGGCTGACGTTTAGCGGTGCTGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGGCAATGACA‑CCGCCCATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGCTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAACGCCCCACTGGTACAGGAGATAACa                                                                      >  1:820249/1‑239 (MQ=255)

                                                                                                                                                                         ccccccAGCGCGGGCGAACGTTAAGCGGTGAAGAAAGGAACCACCACCGTCCCGACAAAGGCGCAACCGATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACaa                                                                     <  1:393342/235‑1 (MQ=255)

                                                                                                                                                                                      gctgACGTTTAGCGGTGATGAACTGAAGCATCACCGTACCGACAAATGCGCAACCGATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGGAGACTTTCGTTTGCGCGTAGACAATAATGGAGGCCCCCAAAGCACCACTGATAACAGCGATAACAAAGGGgtacgtacc                                                        >  2:983969/1‑237 (MQ=255)

                                                                                                                                                                                        cgccGTTGAGGGGTGATAAAAGGAAGCATACCCGGACCGAAAAAGGCGCAACCGATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCgg                                                     <  2:354460/236‑1 (MQ=255)

                                                                                                                                                                                         acgcttaaggggTGATGAAAGGACGACTCCCCGTCCGGACAAAACCGCACCGAATGAAAAGGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCgg                                                     <  2:946267/228‑1 (MQ=255)

                                                                                                                                                                                              ttaggggtaagaaaagaaaGCATACCCGAACCGAAAAGTGCGCAAAAGATGACAAAGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGt                                                <  2:1170614/221‑1 (MQ=255)

                                                                                                                                                                                                       atgaaatgaaGCATCACCGTACCGACAAATGCGCAACCGATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGAGAAATCAATTCCCGTTGACGGGATGGTTTGCATGCAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCACAAAGCCCCACTGATACAGGCGCAAACAAAGGGGTACTTACGCGGCCGGTAGATGCCata                                      >  2:430065/1‑239 (MQ=255)

                                                                                                                                                                                                               aaGCATCACCGTACCGACAAATGCGCAACCGATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCGCTg                              >  2:863613/1‑239 (MQ=255)

                                                                                                                                                                                                                 gCATCACCGTACCGACAAATGCGCAACCGATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCGCTGGt                            >  2:700455/1‑239 (MQ=255)

                                                                                                                                                                                                                  cATCACCGTACCGACAAATGCGCAACCGATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGTAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCGCTGGt                            >  2:760758/1‑238 (MQ=255)

                                                                                                                                                                                                                    tCACCGTACCGACAAATGCGCAACCGATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCg                         >  2:24582/1‑239 (MQ=255)

                                                                                                                                                                                                                     ccccttccgaaCAAATGCGCAACCGTTGTAAAT‑ACAGCCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCgg                        <  1:319319/229‑1 (MQ=255)

                                                                                                                                                                                                                     cACCGTACCGACAAATGCGCAACCGATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCgg                        >  2:281526/1‑239 (MQ=255)

                                                                                                                                                                                                                     aacggcaCCGAAAAATGCGCACACGTTGGAAAGGCTA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAAGACTTGGCAAACCAAAGAAGTAGACTTTCGTTTGCGTGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGTTCTTACGCGGCAGGTTGACGCCATATCCCGCTGGTTCgg                        <  2:34714/233‑1 (MQ=255)

                                                                                                                                                                                                                          tACCGACAAATGCGCAACCGATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGAt                    >  1:753163/1‑238 (MQ=255)

                                                                                                                                                                                                                               aCAAATGCGCAACCGATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGCTGACGCCATATACCGCTGGTTCGGGGAAACCaaa              >  2:1036756/1‑239 (MQ=255)

                                                                                                                                                                                                                               aCAAATGCGCAACCGATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGGGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGCCGCCATATACCGCTGGTTCGGTGATACCaa               >  2:161952/1‑238 (MQ=255)

                                                                                                                                                                                                                                  aaTGCGCAACCGATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTTGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCAtata                                    >  1:789669/1‑214 (MQ=255)

                                                                                                                                                                                                                                  aaTGCGCAACCGATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAg           >  1:1046580/1‑239 (MQ=255)

                                                                                                                                                                                                                                         aaCCGATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGt     <  1:986151/238‑1 (MQ=255)

                                                                                                                                                                                                                                           cggATGACATTGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCaa  <  1:281568/237‑1 (MQ=255)

                                                                                                                                                                                                                                           ccGATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCGATATACCGCTGGTTCGGGGATACCAAACAGACTCGTCa   >  2:332511/1‑238 (MQ=255)

                                                                                                                                                                                                                                           ccGATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCaa  <  1:24582/239‑1 (MQ=255)

CGCACTTCACCAACCGAGGGCAGAATGGCAATACCTTTACCCAACAGGCCACTGGCAAACGTGGTATCAGCGACGTGAATGAGCGGCACAATCTCT‑CCCGTCATCGGTGAACAGATACCGCCCTGCTCAGGTGGTGT‑AATAACC‑TCTGGTGTTTTCTCTT‑GCGGGGCACCCTGCGCTGGCTGACGTTTAGCGGTGATGAAATGAAGCATCACCGTACCGACAAATGCGCAACCGATGGCAATGACA‑CCGCCAATAACGCTGGCCCAGACGGTGAAATCAATTCCCGTTGACGGGATGGTTTGCATGAAGGTGAAAATACTTGGCAAACCAAAGGAGTAGACTTTCGTTTGCGCGTAGCCAATAATGGTGGCCCCCAAAGCCCCACTGATACAGGCGATAACAAAGGGGTACTTACGCGGCAGGTTGACGCCATATACCGCTGGTTCGGTGATACCAAACAGACTCGTCAA  >  NC_000913/3904047‑3904516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A