Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F9 I0 R1 105 139.1 2894862 97.5% 2822490 224.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,815,499 T→G 12.0% D90A (GAC→GCC)  pyrE ← orotate phosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,815,4990TG12.0% 338.3 / 5.6 152D90A (GAC→GCC) pyrEorotate phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (45/81);  new base G (19/3);  total (66/86)
Fisher's exact test for biased strand distribution p-value = 1.04e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 155 total aligned reads displayed.

CTGAATCGCCGAAATCTCGCCGCGCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATC‑CACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGC  >  NC_000913/3815264‑3815734
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                             
cTGAATCGCCGAAATCTCGCCGCGCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGt                                                                                                                                                                                                                                            <  1:557579/239‑1 (MQ=255)
cTGAACCGCCGAAACCCCGCCGCGCCCGCGTCCCTGACGATCGGGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGt                                                                                                                                                                                                                                            <  2:676794/239‑1 (MQ=255)
        ccGAAATCTCGCCGCGCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGGCCCGGTCGTGGTGCTcc                                                                                                                                                                                                                                    >  1:1147725/1‑239 (MQ=255)
            aaTCTCGCCGCGCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCa                                                                                                                                                                                                                                <  2:1237094/239‑1 (MQ=255)
                  gccgcGCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGGCCCGGGCGTGGTGCTCCAcccacgcc                                                                                                                                                                                                                           >  2:423173/1‑232 (MQ=255)
                     gcgcCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAg                                                                                                                                                                                                                       <  1:561790/239‑1 (MQ=255)
                                         cGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCCGGGCGTGAAGCTCCGCCAGTGCCACAG‑CGGGTGGGGTGGCAAACggg                                                                                                                                                                                                  >  1:1443550/1‑239 (MQ=255)
                                         cGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGCCCAGGGCCTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCggg                                                                                                                                                                                                  >  1:70788/1‑239 (MQ=255)
                                          gAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTCAAAGCAGTACGGCAGGGCAAGGTCGTGATGCTCCCCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGGa                                                                                                                                                                                                 <  1:850119/239‑1 (MQ=255)
                                           aGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGTTGCTCCGCCAGTGCCCCAA‑CGGTTGTGGTGGCAATCCGGa                                                                                                                                                                                                 >  1:896722/1‑238 (MQ=255)
                                             cgaatccaccacGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCACCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGGATc                                                                                                                                                                                               <  1:1317420/229‑1 (MQ=255)
                                             cGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGGATc                                                                                                                                                                                               <  1:772955/238‑1 (MQ=255)
                                              gAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGGATc                                                                                                                                                                                               <  1:1345079/237‑1 (MQ=255)
                                                               aaGCGTCGCTCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGcc                                                                                                                                                                              <  1:1175696/237‑1 (MQ=255)
                                                                     cgcgCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAAcagc                                                                                                                                                                       <  1:412245/238‑1 (MQ=255)
                                                                       cgcCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCCAGGCGTGATGCCCCCGCCGGGCCCAAA‑CGGTTTTGGGGGGCATCGGGATCCCTTTGTAAGAACGGGCAAAcagaggg                                                                                                                                                                    >  2:167591/1‑235 (MQ=255)
                                                                              ggCCTGAATAATCTCCATCGACTCGCGAATCGCCGTGCCGGCGGTGATCACATCAGCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCagggctggatcaccgca                                                                                                                                                             <  1:1068784/239‑17 (MQ=255)
                                                                               gCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAggg                                                                                                                                                                                <  1:1315478/219‑1 (MQ=255)
                                                                                    aataatCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGt                                                                                                                                                                                                                                            <  1:222535/155‑1 (MQ=255)
                                                                                    aataatCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGGCCCGGGCCTGATGCTCCCCCCGGTCCACAGCCGGTTGGGGGGGCAACGGGGATCCCTTT‑TAAACAAGGGCAAAcagccagtcggacacaaat                                                                                                                                                        >  2:196735/1‑223 (MQ=255)
                                                                                     ataatCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGc                                                                                                                                                       <  2:66527/238‑1 (MQ=255)
                                                                                     ataatCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGc                                                                                                                                                       <  1:175807/238‑1 (MQ=255)
                                                                                         tctcCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCCGGTCGTGATGCTCCCCCAGTGCCACCG‑CGGTTGTGGTGGCAATCGGGGTCCCCTTGTAAGCAGGGGCCAACAGCAGATCGAACTCAATGcccgga                                                                                                                                                  >  1:1261381/1‑235 (MQ=255)
                                                                                         tctcCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATc                                                                                                                                                                  >  1:1161235/1‑223 (MQ=255)
                                                                                         tctcCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATc                                                                                                                                                                  <  2:1161235/223‑1 (MQ=255)
                                                                                         tctcCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGaa                                                                                                                                                  >  1:945523/1‑239 (MQ=255)
                                                                                         tctcCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCGTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGaa                                                                                                                                                  <  1:759772/239‑1 (MQ=255)
                                                                                          ctcCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCGCCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAAt                                                                                                                                                 <  1:24735/239‑1 (MQ=255)
                                                                                            ccATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAGCCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATc‑c                                                                                                                                              <  1:557004/239‑1 (MQ=255)
                                                                                              aTCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCCGTTCCACAG‑CGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAACAGATCGAACTCAATGCCGGGATc‑cc                                                                                                                                             >  1:474173/1‑237 (MQ=255)
                                                                                              aTCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGGCCCGGCTGTGACGcctctc                                                                                                                                                                                                                                  >  2:222535/1‑150 (MQ=255)
                                                                                              aTCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGGGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGGACAGGGCGTGATTCTCCGCCAGTGCCACAG‑CGGTTGGGGTGGGCATCGGGATTCCTTTTTAAGCAGGGCCCAACAGCAGCTAGCACTAAATGCCGGAATc‑cc                                                                                                                                             >  2:1010078/1‑237 (MQ=255)
                                                                                                      gcgAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATC‑CACCAACGCtt                                                                                                                                    <  1:153681/239‑1 (MQ=255)
                                                                                                                  tCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATC‑CACCAACGCTTCAGCGTaaaaa                                                                                                                         <  1:386142/238‑1 (MQ=255)
                                                                                                                  tCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATC‑CACCAACGCTTCAGCGTaaaaa                                                                                                                         <  1:830935/238‑1 (MQ=255)
                                                                                                                     ggctgTCATCACATCATCTACCAGCAGTGCGCGTCCTTGTAACGCGCTACCATCCAGATTGCCGCCTTCACCGTGGGCTTTTGCTGCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATC‑CTCCAACGCTTCAGCGTAAAAACGGc                                                                                                                     <  2:896722/239‑1 (MQ=255)
                                                                                                                          tgatacaatcatcTACCAGGATTCCGCCTCCTTGGAAGGCGTTACCACCCAGATTGCCGCCTTCACCGTGGTCTTTTTCTGCTTTGCGTTTCAAGCAGTACGGGAGGTCCGGGTCGAAATGCTCCGGCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGGCGGAATC‑CACCAACGCTTCAGCGTAAAAACGGCCTaa                                                                                                                 <  2:94628/231‑1 (MQ=255)
                                                                                                                          tGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATC‑CACCAACGCTTCAGCGTAAAAACGGCCTaa                                                                                                                 <  2:1044026/238‑1 (MQ=255)
                                                                                                                          tGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATC‑CACCAACGCTTCAGCGTAAAAACGGCCTaa                                                                                                                 <  2:805129/238‑1 (MQ=255)
                                                                                                                          tGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATC‑CACCAACGCTTCAGCGTAAAAACGGCCTaa                                                                                                                 <  2:1313340/238‑1 (MQ=255)
                                                                                                                          tGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATC‑CACCAACGCTTCAGCGTAAAAACGGCCTaa                                                                                                                 <  2:945523/238‑1 (MQ=255)
                                                                                                                          tGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTGCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATA‑CACCAACGCTTCAGCGTAAAAACGGCCTaa                                                                                                                 <  1:820238/238‑1 (MQ=255)
                                                                                                                          gcatcacatcacCTACCCGCATTACTCGTCCTTGTAACGCGCTCCCAACCATATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATC‑CACCAACTCTTCAGCGTAAAAACGGCCTaa                                                                                                                 <  2:42951/236‑1 (MQ=255)
                                                                                                                           gATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATC‑CACCAACGCTTCAGCGTAAAAACGGCCTAACa                                                                                                               <  1:1035436/239‑1 (MQ=255)
                                                                                                                            aTCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGGCGTGATGCCCCGCCCGGGCCACAG‑CGGTTGTGGTGGGCATCGGGGTCCCCTTGTTAGCAGGGCCCAACAGCAGATCGGACTCAATGCCGGAATC‑CACCAACGCTTCAGCGTAAAAACGGCCTAACAg                                                                                                              >  2:77997/1‑239 (MQ=255)
                                                                                                                              cacaTCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAggg                                                                                                                                                                                <  1:1334318/172‑1 (MQ=255)
                                                                                                                                   catcTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCCGGTCGCGATGCCCCGCTCGTGCCCCAG‑CGGTTGAGGTGGCAATCCGGACACCTTTGTAAGCGGGGCCAAACAGCAGATCGAATTCAATTCCGGAATC‑CACCAACCCCTCACCGTAAAAACGGCCCAACAATGcacc                                                                                                        >  2:1135296/1‑235 (MQ=255)
                                                                                                                                      cTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATC‑CCCCAACGCTTCAGCGTAAAAACGGCCCAAAAGTGCCAGATc                                                                                                     >  1:545729/1‑238 (MQ=255)
                                                                                                                                      cTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGGCCGGGTCGTTATGCTCCGCCCGTGCCACCG‑GGGTTGTGGCGGCAATACGTATCCCCTTTTAAGCCTGCGCACACATGAGATAGGACTCCATGCCAGAATC‑CTCCAAAGCCTCAGCGTAACAACAGACCAACAgatccaaata                                                                                                     >  2:508955/1‑229 (MQ=255)
                                                                                                                                      cTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGCGCCAGGTCGTGATGCTCTGCCAGTGCCACAC‑GGGTTGGGGGGGCAATCGGGATCCCTTTGTAAGCCTGGCCTAAAAGCGCATCGGACTCAATGGCGGAATCTCACAAAC‑CTTACGCGTAAAAATCGCCCAAAAATGCCTGa                                                                                                       >  2:840225/1‑236 (MQ=255)
                                                                                                                                      cTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCATGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGGAACCCTTTGTAAGCAGTGCCAAACAGCAGAACGAACTAAATGCCGGGATC‑CCACAACGCTTCCGCGGAAAAACGGCCTAAAAGTGCCAGATc                                                                                                     >  1:964175/1‑238 (MQ=255)
                                                                                                                                        aCCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAG‑CCGGTGTGGGGGCCATCGGGATCCCTTTGTAAGCAGGGCCAAAcagca                                                                                                                                                                      >  2:1334318/1‑172 (MQ=255)
                                                                                                                                        aCCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCCCCCCCCGTGCCACAG‑CGGTTGGGGTGGCCATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATC‑CACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATccc                                                                                                   >  1:24247/1‑236 (MQ=255)
                                                                                                                                          ccgCATTACGCGTCCTTGTAACGCTCTACCAACCAGATTGCCGCCTTCGCCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATC‑CACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGcc                                                                                                <  1:329511/237‑1 (MQ=255)
                                                                                                                                          cAGCATTCCGCGTCCTTGTACCGCGCCACCATCCAGATTGTCGCCTTCACCGTGGTATTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGAGGTGGCATTCGGGAGCCCTTCGTAAGCAGGGACAAACAGCAGATCGAACTCAATGCCGGAATC‑CACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGcc                                                                                                <  2:550940/239‑1 (MQ=255)
                                                                                                                                          cAGCATTACGCGTCCTTTTAACGCGCTCCCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTGCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATC‑CACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGcc                                                                                                <  2:1236783/239‑1 (MQ=255)
                                                                                                                                          cAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATC‑CACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGcc                                                                                                <  1:1240244/239‑1 (MQ=255)
                                                                                                                                          cAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATC‑CACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGcc                                                                                                <  2:823612/239‑1 (MQ=255)
                                                                                                                                          cAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATC‑CACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGcc                                                                                                <  1:947648/239‑1 (MQ=255)
                                                                                                                                          cAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATC‑CACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGcc                                                                                                <  1:1231189/239‑1 (MQ=255)
                                                                                                                                          cAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATC‑CACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGcc                                                                                                <  1:196735/239‑1 (MQ=255)
                                                                                                                                          cAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATC‑CACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGcc                                                                                                <  1:474248/239‑1 (MQ=255)
                                                                                                                                          cAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATC‑CACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGcc                                                                                                <  2:626893/239‑1 (MQ=255)
                                                                                                                                          aaGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATGGCCGCCTGCTCCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGGCGTGAGGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATC‑CACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGcc                                                                                                <  2:31800/238‑1 (MQ=255)
                                                                                                                                             cATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCg                                                                                                                                                     <  2:271285/184‑1 (MQ=255)
                                                                                                                                             cATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCg                                                                                                                                                     >  1:271285/1‑184 (MQ=255)
                                                                                                                                             cATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATC‑CACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGcc                                                                                                >  1:619186/1‑236 (MQ=255)
                                                                                                                                             cATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATC‑CACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCgg                                                                                             >  2:650135/1‑239 (MQ=255)
                                                                                                                                             cATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGGCGTGATGCTCCTCCAGTGCCACAG‑CGGTTGTGGTGGCAAACGGGATCCCTTTGTACGCAAGGCCAAACAGCAGATCGAACTCAATGCCGGAATA‑CACCAACGCCTCAGCGTAAAAACGGCCCAAAAGTGCCAGAACGCGccccg                                                                                             >  1:393258/1‑237 (MQ=255)
                                                                                                                                               ttACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGGCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCCGATCGAACTCAATGCCGGAATC‑CACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCggga                                                                                           >  1:854413/1‑237 (MQ=255)
                                                                                                                                                         ttGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATC‑CACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGcc                                                                                 <  1:423173/239‑1 (MQ=255)
                                                                                                                                                          tGTAACGCGCTACCAACCAGATTGCCGCTTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCCGGATCCCTTTGTAAGCAGGGCCCAAAAGCAGATCGGACTCAATGCCGGAATA‑CACCAAAGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGAACCCGCCCGGTATTAAACAGcc                                                                                 >  2:1272051/1‑238 (MQ=255)
                                                                                                                                                               cgcgCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATC‑CACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGt                                                                           <  1:1279614/239‑1 (MQ=255)
                                                                                                                                                                 cgcTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATC‑CACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTg                                                                         >  1:1351564/1‑239 (MQ=255)
                                                                                                                                                                 cgcTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATC‑CACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTg                                                                         >  1:1261507/1‑239 (MQ=255)
                                                                                                                                                                 cgcTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATC‑CACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGCTg                                                                         >  1:367780/1‑239 (MQ=255)
                                                                                                                                                                 cgcTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGGCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCCAACAGCAGATCGAACTCAAAGCCGGAATC‑CACCAACGCTTCAGCGTAAAAACGGCCTAACAGGGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTg                                                                         >  1:1297402/1‑239 (MQ=255)
                                                                                                                                                                  gcTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATC‑CACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTg                                                                         <  1:63197/238‑1 (MQ=255)
                                                                                                                                                                   cTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCCGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATC‑CACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTgaa                                                                       >  2:90331/1‑239 (MQ=255)
                                                                                                                                                                          ccaGACTGCCGCCACAGCCGTGGTCGTTTGCTTCTTTGCGGGGAAAGCAGTACGGCAGGTCCAGGCCGTGAGGCGCCGCCAGTGGCACAG‑CGGTAGTGGTGGCAATCGGAACCCCAGCGCAAACAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATC‑CACCAACGCATCAGCGTAAAAACGGCCTAACAGAGCACGTTGGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAagag                                                                <  2:297959/239‑1 (MQ=255)
                                                                                                                                                                                tGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGGATCCCTTTGTAAg                                                                                                                                                                                     >  1:997490/1‑117 (MQ=255)
                                                                                                                                                                                tGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATC‑CACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCtt                                                          >  1:1134681/1‑239 (MQ=255)
                                                                                                                                                                                 gccgccTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATC‑CACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGCAGAAATAAGGGCttt                                                         >  1:494907/1‑239 (MQ=255)
                                                                                                                                                                                  ccgccCTCACCGTGGTCTTTTGTTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCCGGTCGGGATGCTCCGTCCGTGGCACAG‑CGCGTGTGGTTGCCATCGCGAACCCTTTGTTAGCAGGGCCACACACCAGACCGAACTCCATGCCCGATTC‑CAACAACACCTTCGCGAAAAAAAGGCCTAACCAGACCCGATCCAGCCAGGTATTAAACAGCCCCGCCGCGCACGAATAGCGGCtttt                                                        >  2:906801/1‑239 (MQ=255)
                                                                                                                                                                                     ccTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATC‑CACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTgc                                                      >  1:1427538/1‑238 (MQ=255)
                                                                                                                                                                                        tCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATC‑CACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTTCGccc                                                  >  1:583536/1‑239 (MQ=255)
                                                                                                                                                                                        tCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGCGATGCTCCGCCAGTGCCACAG‑CGGTTGGGGTGGCAATCGGGATCCCTTTTTAAGCAGGGCCAAACAGCCGATCGAACTCAATGCCGGCATC‑CACCAACGCTTCAGCGGAAAAACGGCCTAACAGTGCCAGATCCCGCCCGGTATTAAACAGCCCGGCGTTTAAGAAATAAGGGCTTTTGCGccc                                                  >  1:1370721/1‑239 (MQ=255)
                                                                                                                                                                                         cACCTTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTCCGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATC‑CACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCg                                                 <  2:1316334/239‑1 (MQ=255)
                                                                                                                                                                                                ggcTTTTGCTTCTTTGCGGTTAAGGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATC‑CACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTc                                           <  1:906801/236‑1 (MQ=255)
                                                                                                                                                                                                gTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCCGGGCGTGGTGCTCCCCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGGATCCCTTTTTAAGCAGGGCCACACAGCCGAGCGAACTCAATGCCGGAATC‑CACCAACGCTTCAGCGCAAAAACCGCCTAACAGGGCCAGATCCCGCCCGGGATTTAACAGCCCCGCGGTGAAGAAATAAGGGCCTTTGCGCCCGGGTTTc                                           >  2:1161112/1‑238 (MQ=255)
                                                                                                                                                                                                gTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGCCGTGATGCCCCGACAGTGCCAAAG‑CGGTTGTGGTGGCAATCGGGGTCCCTTTGTAAGCAGGGCCAAACAGCAGATCGGACTCAATGCCGGAATC‑CACCAACGCCTCAGCGTAAAAACGGCCTAACAGGGCCAGATCGCGCCCGGTATTAAACAGACCGGCGTTGAAGAAATAGGGGCTTTTTGGCCCCGCTTTCa                                          >  2:1256575/1‑239 (MQ=255)
                                                                                                                                                                                                  cttttgtctcgttcgtgtcaaaGCAGTCCGGCAGTCACACGTCGGTATGCTCCGCCAGTGCCACAG‑CGGTTGTCGTTGCTATCGGGAGCCCTTTGTAAGCAGGGCCAGACAGCAGATCGAACTCAATGCCGGAATC‑CACCATCTCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGc                                        <  2:582947/220‑1 (MQ=255)
                                                                                                                                                                                                   ttttGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAAACGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATC‑CACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAAGGGCTTTTGCGGCCGGCTTTTAGCg                                       >  2:169720/1‑239 (MQ=255)
                                                                                                                                                                                                      tGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCCCAG‑CGGTTTGGGGGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATC‑CACCAACGCTTCCGCGTAAAAACGGCCTAACAGCGCCAGATCGCGCCCGGTATTAAACAACCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAACgcta                                    >  1:1413505/1‑236 (MQ=255)
                                                                                                                                                                                                           cttTGCGGTTAAAGCAGTACGGCAGGTCCCGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGGGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATC‑CACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGt                                                                           >  1:54092/1‑195 (MQ=255)
                                                                                                                                                                                                           cttTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGTATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATC‑CACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTc                                >  1:1311385/1‑238 (MQ=255)
                                                                                                                                                                                                             ttGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCGGATCGAACTCAATGCCGGAATC‑CACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGcc                                                                                                <  2:503852/172‑1 (MQ=255)
                                                                                                                                                                                                              tGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATC‑CACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCa                            <  1:169720/239‑1 (MQ=255)
                                                                                                                                                                                                                             aCGGCAGGTCCAGGTCGTGATGCTCCCCCCGTGCCACAG‑CGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGCATC‑CACCAACGCTTCAGCGCAAAAACGGCCTAACAGTGCCAGAACGCCCCCGGGATTAAACAGCCCGGCGGTgaa                                                                       >  2:993470/1‑181 (MQ=255)
                                                                                                                                                                                                                                     tccaggtcGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATC‑CACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTa       <  1:1275674/237‑1 (MQ=255)
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                             
CTGAATCGCCGAAATCTCGCCGCGCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAG‑CGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATC‑CACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGC  >  NC_000913/3815264‑3815734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A