Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F18 I0 R1	274	115.5	2655328	86.7%	2302169	226.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,065,570	T→A	16.4%	T66S (ACC→TCC)	yihO ←	putative sulphoquinovose importer

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,065,570	0	T	A	16.4%	229.0 / 7.5	106	T66S (ACC→TCC)	yihO	putative sulphoquinovose importer
Reads supporting (aligned to +/- strand): ref base T (62/21); new base A (4/15); total (68/38)
Fisher's exact test for biased strand distribution p-value = 2.18e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 106 total aligned reads displayed.

ATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACA‑GCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGGAACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAACT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTTTAT  >  NC_000913/4065347‑4065802

atcGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACA‑GCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGGAACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑Taaaaa                                                                                                                                                                                                                              >  2:13908/1‑238 (MQ=255)

     aCCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACA‑GCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGGAACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAACT‑TCg                                                                                                                                                                                                                       <  2:864021/239‑1 (MQ=255)

      cctaagatggttttaaagaaaaggTATGAAAGTCAAAACAT‑AAAAACAGGGGGGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGGAACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAACT‑TCGc                                                                                                                                                                                                                      <  1:265810/216‑1 (MQ=255)

      ccAGACGAACAGTTCAACAGACTGTACGAAAGTCCAAACATCATAAACA‑GCCCGGTGGCGATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGGAACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAACT‑TCGc                                                                                                                                                                                                                      <  2:373574/239‑1 (MQ=255)

       cATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACA‑GCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGGAACCGATGCATCTAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGCAAAAATCCGGTGA‑GCATATAGGTAAAC‑GCGG‑TAAAAAACT‑TCGcc                                                                                                                                                                                                                     >  2:182762/1‑239 (MQ=255)

         tacggtgagtttctcagctgtgaTGAAAGGCCAAAAATCATAAAGA‑GCGGGGTGGCAATGGGTGGTTTAACCGGCAAACAAAAGGGGGGGGAG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGGAACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAACT‑TCGccac                                                                                                                                                                                                                   <  1:876345/217‑1 (MQ=255)

         tACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACA‑GCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGGAACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TCAAAAAAT‑TCGcga                                                                                                                                                                                                                    >  2:821104/1‑236 (MQ=255)

         tACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACA‑GCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGGAACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAACT‑TCGcca                                                                                                                                                                                                                    >  2:1080995/1‑238 (MQ=255)

          aCGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACA‑GCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGGAACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAG‑GCGG‑TAAAAAACT‑TCGccacc                                                                                                                                                                                                                  >  2:1235587/1‑239 (MQ=255)

            gAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACA‑GCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGGAACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAACT‑TCGCCACCAg                                                                                                                                                                                                                <  2:1141864/239‑1 (MQ=255)

            acgCAGTTCAACAGACTGTTTGAAAGACCAAACATCATAAACA‑GCGCGGTTGCAATCGTCGTTTTAACCGGCAACAAAAAGGTGGTGGCG‑‑ATAAATTGGAGCG‑TTGCAATTAACGCCGC‑CGGAACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTGTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAACT‑TCGCCTCCAg                                                                                                                                                                                                                <  1:13908/237‑1 (MQ=255)

               cAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACA‑GCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGGAACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAACT‑TCGCCACCAGaaa                                                                                                                                                                                                             >  2:38619/1‑239 (MQ=255)

                 gTTCATCAGACTGTATGAAAGTCCAAACATCATAAACA‑GCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGGAACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑GGGCC‑CAATATTTTTCCG‑CGA‑GTAGGGTAAAAATCCGGGAA‑GCAAATCGGAAAAC‑GCGG‑TAAAAAACT‑TCGCAACAAAAAAGa                                                                                                                                                                                                           >  1:651645/1‑239 (MQ=255)

                 gTTCATCAGACTGTATGAAAGTCCAAACATCATAAACA‑GCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGGAACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATCTCGGTAAAC‑GCGG‑GAAAAAACT‑TCGCCACCACaaaaa                                                                                                                                                                                                           >  1:1141864/1‑237 (MQ=255)

                 gTTCATCAGACTGTATGAAAGTCCAAACATCATAAACA‑GCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGGAACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAACT‑TCGCCACCGGAAAGa                                                                                                                                                                                                           >  2:697260/1‑239 (MQ=255)

                 gTTCATCAGACTGTATGAAAGTCCAAACATCATAAACA‑GCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGAGGAGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACTCCGC‑CGGAACTGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGT‑TAAAAAACT‑TCGCCACCATAAAGa                                                                                                                                                                                                           >  2:752005/1‑239 (MQ=255)

                    tattagattttataaatataaaaaCATGATAAACA‑GGGGTGTGGCTAGCGTCTTTTAAAGCGG‑AAA‑AAAAGGTGGGGGGGGAAAAAACGGGGGTG‑TGTAAAATAACCCCGC‑CGGAACCGTGTAATAAAAAAAAAA‑G‑GGAGAAATTTTGCTTTT‑AGGCA‑AAATATTTTCCGG‑AGA‑GTCGAGAAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGT‑TAAAAAACT‑CCGCCACCAAAAAGAT‑aa                                                                                                                                                                                                       <  1:293988/218‑1 (MQ=255)

                    catcaGACTGTATGAAAGTCCAAACATCATAAACA‑GCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGGAACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGTGTAAAAATCCGGTGA‑GCATATCGGAAAAC‑GCGG‑TAAAAAAAT‑TCGCCACCAAAAAGaa‑aa                                                                                                                                                                                                       >  1:959432/1‑239 (MQ=255)

                            tGTATGAAAGTCCAAACATCATAAACA‑GCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGGAACCGCTGCATATAACATAAA‑G‑GGACAAAATTTGCCTTT‑AAGCC‑CAATATTTTTCCG‑CGA‑GTCGAGAAAAAAACCGGGAA‑GCGTAACGGGAAAC‑GCCG‑GAAAAAAAT‑TCCCCAACAAAAAGaa‑aacacccaca                                                                                                                                                                                               >  1:1304304/1‑231 (MQ=255)

                            tGTATGAAAGTCCAAACATCATAAACA‑GCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGGAACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAACT‑TCGCCACCAGAAAGAT‑AATCCCGCCa                                                                                                                                                                                               >  2:935501/1‑239 (MQ=255)

                            tGTATGAAAGTCCAAACATCATAAACA‑GCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGGAACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCAGGTAAAAATCCGGTGA‑GCATATCGGAAAAG‑CCGG‑TAAAAAACT‑TCGCAACAAGAAAGAT‑AATCCCGCCa                                                                                                                                                                                               >  2:587945/1‑239 (MQ=255)

                              tATGAAAGTCCAAACATCATAAACA‑GCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCG‑‑ATAAACTGGATCG‑TTGCAATTAACGCCGC‑CGGAACCGATGCGTATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCT‑CGA‑GTCGAGTAAAAAACCGGGTA‑GCATATCGGCAAAC‑ATGG‑TAAAAAACT‑TGCCCAACAAAAAGAC‑aaacaccccaca                                                                                                                                                                                             >  1:718950/1‑229 (MQ=255)

                              tATGAAAGTCCAAACATCATAAACA‑GCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGGAACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTGAAC‑GCGG‑TAAAAAACT‑TCGCCACCAGAAAGAT‑AATCCCGCCATa                                                                                                                                                                                             >  2:330125/1‑239 (MQ=255)

                              tATGAAAGTCCAAACATCATAAACA‑GCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGGAACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAACT‑TCGCCACCAGAAAGAT‑AATCCCGCCATa                                                                                                                                                                                             >  2:293988/1‑239 (MQ=255)

                                      tCCAATCATCATAAACA‑GCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGGAACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAACT‑TCGccac                                                                                                                                                                                                                   <  2:793493/210‑1 (MQ=255)

                                      tCCAATCATCATAAACA‑GCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGGAACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAACT‑TCGccac                                                                                                                                                                                                                   >  1:793493/1‑210 (MQ=255)

                                        cAAACATCATAAACA‑GCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGGAACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAACT‑TCGCCACCAGAAAg                                                                                                                                                                                                            >  2:265810/1‑215 (MQ=255)

                                        cAAACATCATAAACA‑GCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGGAACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAACT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGc                                                                                                                                                                                   >  2:271177/1‑239 (MQ=255)

                                        cAAACATCATAAACA‑GCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGGAACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGGGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAACT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGc                                                                                                                                                                                   >  2:1177027/1‑239 (MQ=255)

                                        cAAACATCATAAACA‑GCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGGAACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAAAATTTTTCCG‑AGA‑GTCGAGTAAAAATCCGGTGA‑GCAAACCGGTAAAC‑GCGG‑TAAAAAACT‑TCGCCACAAAAAAGAA‑AATCCCGCCaaaaaaagaagga                                                                                                                                                                                   >  1:428683/1‑227 (MQ=255)

                                          aaCATCATAAACA‑GCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGGAACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGC‑GCATATCGGTAAAC‑GCGG‑GAAAAAACT‑TCGCCACCGGAAAGAT‑AATCCAGCCATAGTAAGCAGGCa                                                                                                                                                                                  >  2:907809/1‑238 (MQ=255)

                                               catAAACA‑GCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGGAACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAACT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCACCCCTa                                                                                                                                                                            >  2:564390/1‑239 (MQ=255)

                                                atAAACA‑GCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGGAACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGGGA‑GCATAACGGTAAAC‑GCGG‑TCAAAAACT‑TCGCAACCAGAAAAAA‑AATCCCGcaacacaaaaaaggcaacccaaa                                                                                                                                                                           >  1:21196/1‑217 (MQ=255)

                                                        gcgcgggggccatttcttttttTACCGGGAAAAAAAAGGGGGGGGCGA‑ATAAAATGGAGGG‑TTGAAATTACCCCCGC‑CGGACCCGTTGCATAAAAAATAAA‑G‑GGACAAAATTTGCCTTT‑AGGCC‑CATTATTTTTCCG‑CGA‑GTCGATAAAAAATCCGGTGA‑GCATACGGGTAACC‑GCGG‑AAAAAATCT‑TCGCCACAAGAAAAAA‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATc                                                                                                                                                                     <  1:595067/221‑1 (MQ=255)

                                                        gcgcGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGGAACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAACT‑TCGCCACCCGAAAGAT‑AATCCCGCCATAGTAACCAGGCACCCCTAATTCATCg                                                                                                                                                                    >  2:34859/1‑239 (MQ=255)

                                                        gcgcGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGGAACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAACT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAAtt                                                                                                                                                                         >  2:861180/1‑234 (MQ=255)

                                                        gcgcGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGGAACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAACT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCg                                                                                                                                                                    >  2:458218/1‑239 (MQ=255)

                                                        gcgcGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGGAACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GAATATCGGTAACC‑GCGG‑TACAAAAAT‑TTCCACCCAGAAAGAA‑AATCCCCCCAAAATAAGCAGCAAACCCTAAATcccag                                                                                                                                                                    >  1:49615/1‑235 (MQ=255)

                                                        gcgcGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGGAACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAAGATTTTTCCG‑CGA‑GTCGATTAAAAAACCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAACT‑TCGCCACCACAACGAA‑AATCCCGCCAAAGAAAGAAAGCACACCTaaacaacag                                                                                                                                                                    >  1:413082/1‑232 (MQ=255)

                                                         ctcgGTGGCAATCGTCGTTTTAACCGGCAAACAACAGGTGTCGGCG‑‑ACAAACTCGAGCG‑TTGCAATTCACGCCGC‑CGGACCCGCTGCCAACAAAATAAA‑G‑GGCCGAAATTTGCCTTT‑AGCCC‑CAAAATTTTTCCG‑AGA‑ATCGGGACAAAATCCGCGGT‑TCATACCGCTAAAC‑CCGG‑AAAAACACT‑TCACCACCCAGCAGAT‑AACCCCGCCATAGAAAGAAggacaccctacatacacgg                                                                                                                                                                   >  1:1316058/3‑222 (MQ=255)

                                                         cgcgGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGGAACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAACT‑TCGCCACCAGAAAGGT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCgg                                                                                                                                                                   >  2:881549/1‑239 (MQ=255)

                                                         cgcgGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGGAACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAACT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCgg                                                                                                                                                                   >  2:595067/1‑239 (MQ=255)

                                                         cgcgGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGGAACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAACT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCgg                                                                                                                                                                   >  2:746514/1‑239 (MQ=255)

                                                         cgcgGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCG‑‑ACAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGGAACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAc‑g                                                                                                                                                                                                                                        >  2:549957/1‑173 (MQ=255)

                                                         cgcgGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCG‑‑ACAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGGAACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAc‑g                                                                                                                                                                                                                                        <  1:549957/173‑1 (MQ=255)

                                                          gcgGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGGAACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAACT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGt                                                                                                                                                                  >  2:1300143/1‑239 (MQ=255)

                                                           cgGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGGAACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAACT‑TCGCCACCAGAAAGAT‑AATCCGCCCTAAGTAAGCAGGCACCCCTAATTCATCGGta                                                                                                                                                                 >  2:501482/1‑239 (MQ=255)

                                                             ggtccactcgttgTTTTACCCGGAAAACAAAGTGTGGTTGAG‑‑AAAAACTGGACCG‑TTGAAATTAACCCCGC‑CGGACCCGTAGCATAAAAAGTAAA‑G‑GAAAAAATTTTGCCTTT‑GGCCC‑AAATATTTGTCCG‑CGA‑GTCGAAAAAAACTCCGGTGA‑TCATATCGGTAAAC‑GAGA‑TAAAAACCT‑TCTCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGtat                                                                                                                                                                <  1:1300143/231‑1 (MQ=255)

                                                                      tgttttttAACCGCCAAAAAAAGGGGGGGGGCA‑‑ATAAAAGGGAGCG‑TTACAATTACCCGCGC‑AGGACCCGCTTAAAAAAAAAAAAA‑G‑GGAAGAAATTTGGCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑TACGAATAAAAAGCCGGTGA‑AAATATCGTAAAAC‑GTGG‑TAAAAATCT‑TCGCCACCAGAAAGAT‑AACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTgag                                                                                                                                                      <  1:716765/235‑1 (MQ=255)

                                                                      gtttttttAACCGCAAAAGAAAATGGGGGGGCA‑‑AAAAAATGGAGTG‑GATCAATTACGCCCGC‑CGGAACCG‑TGTAAAAAAAAAAAAGG‑GGAAGAATTTGTCGTTT‑AGGCC‑CAATATTTGCCCG‑GGA‑GTCGAATAAAAACCCGGTGA‑TAATATCGGAAACC‑GCGG‑AAAAAATCT‑CCGCCACAAAAAAAAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTgag                                                                                                                                                      <  1:427868/235‑1 (MQ=255)

                                                                       ttgtTTTTACCCGGAAAAAAAAGGGGGTGGGG‑‑AAAAAAGGGGGCG‑TTGCAAATTACGCCGCGCGAAACGGTTGATTAAAAAAAAAA‑G‑GGA‑AAAATTTGGCTTTGAGGCC‑AAATATTTTTGCG‑CGA‑GTGGAGTAAAAACCCGGTGA‑GCTTTTCGGAAAAC‑GCGG‑AAAAAACCT‑TCGCCACCAAAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTgag                                                                                                                                                      <  1:1257455/237‑1 (MQ=255)

                                                                        cgtTTTAACCGGCAAACAAAAGGTGGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGGAACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAACT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTTTAAAATTTgagga                                                                                                                                                    >  2:361330/1‑239 (MQ=255)

                                                                                ccGGCAAACAAAAGGTGGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGGAACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAACT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTgaggagcaaaaag                                                                                                                                            >  2:763914/1‑232 (MQ=255)

                                                                                 cGGAAAACAAAAGGTGGT‑GCC‑‑AAAAACTGGGGGGTTTGCAATAAACGCCGC‑CGAAACCGCTGAAAATAAAATAAA‑G‑GGACGAATTTTGCCTTT‑AGGCC‑AAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑AAAAAAACT‑TCGCCACAAAAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACagag                                                                                                                                           <  2:206443/239‑1 (MQ=255)

                                                                                    caaacaaaAGGTGGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGGAACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAACT‑TCGCCACCAGAAAGAT‑AATCCCGCCACAGTAAGCAGGCAACCCTACTTCATCGGAGTAAAATTTGAGGAAATaaaaagaa                                                                                                                                         >  1:1179303/1‑231 (MQ=255)

                                                                                                ggggggccaaaaacggggggtg‑TTACAATTACCCCCGC‑GGAACCCGCTGATTAAAAAAAAAG‑G‑GTAAGAATTTTGCCTTA‑GGGCC‑CAATATTTCGCCG‑CGA‑GTCGAGAAAAAAGCCGGTGA‑GCATTGCGGTAAAC‑GAGA‑TAAAAAACT‑CCGCCACAAAAAAGAA‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCag                                                                                                                                <  1:697260/216‑1 (MQ=255)

                                                                                                 gtggCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGGAACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAACT‑TCGCCACCAGAAAGAT‑AATCCCGCCATCGTAAGAAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCAt                                                                                                                           >  2:796883/1‑239 (MQ=255)

                                                                                                 gtggCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGGAACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAACT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATACAATTTGAGGAGATACAGGGTACCGCAGCAGAGCAt                                                                                                                           >  2:463469/1‑239 (MQ=255)

                                                                                                   ggCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGGAACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAACT‑TCGCCACCAGa                                                                                                                                                                                                               <  2:54502/154‑1 (MQ=255)

                                                                                                   ggCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGGAACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAACT‑TCGCCACCAGa                                                                                                                                                                                                               >  1:54502/1‑154 (MQ=255)

                                                                                                         aTAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGGAACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAACT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGGATAAAATTTCAGGAGATCCACAGTACCCGTGCCGAGCATTAAAt                                                                                                                      >  2:1285478/1‑238 (MQ=255)

                                                                                                          tAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGGAACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAACT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTc                                                                                                                    >  2:543928/1‑239 (MQ=255)

                                                                                                           aaaCTGGAGCG‑TTGCAATTAACGCCGC‑CGGAACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAACT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCg                                                                                                                   >  2:901282/1‑239 (MQ=255)

                                                                                                                 gAGCG‑TTGCAATTAACGCCGC‑CGGAACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAACT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGGATAAAATTTGAGGAGATACAAAGAACCGATGCAGACAATTAAATTGAAAcca                                                                                                              >  1:268499/1‑237 (MQ=255)

                                                                                                                     ttttgaaataacacccgc‑cgGACCCGCTGAAAAAAAAAAAAG‑G‑GGACAAAATTT‑CCTTT‑TAGCC‑CAAATTTTTTCCG‑GAAGGGCGAGAAAAAACCCGGGAG‑GAATACCGGAAAAC‑GCGG‑TAAAAAACT‑TCCCCACAAAAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACg                                                                                                           <  2:684656/224‑1 (MQ=255)

                                                                                                                       ttGCAATTAACGCCGC‑CGGAACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAACT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGGAATAAAAAGTACCGATGCAGAGAATTAAATTCGAAacgacgac                                                                                                         >  1:526885/1‑236 (MQ=255)

                                                                                                                        tGCAATTAACGCCGC‑CGGAACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAACT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCg                                                                                                        >  2:447570/1‑238 (MQ=255)

                                                                                                                                 cgccgc‑CGGAACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAACT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTAATCGGTATACAATTTGAGGAGATACAGAGTACCg                                                                                                                                      >  2:891897/1‑199 (MQ=255)

                                                                                                                                  gccgc‑cGGAACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAACT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTAACGATGAAGAGAATTAAATTCGAACCGAAGTCCCccaaacaaaa                                                                                             >  1:299098/1‑235 (MQ=255)

                                                                                                                                      c‑cgGAACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAACT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCTAACCGAAGGCCCccaaacaataaggg                                                                                         >  1:723233/1‑235 (MQ=255)

                                                                                                                                                   taaataaaaaaaa‑GAGGAGAAAATTTTCCTTT‑GAGCC‑CAAATTTTTTCCGTGGA‑GTCGAGTAAAAATAGTGTGA‑GCAT‑GCAGAAAAA‑GCGG‑AAAAAAATTCCCGCACCCAAAAAGAA‑ACGCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTccc                                                                               <  1:15198/236‑1 (MQ=255)

                                                                                                                                                    atatAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAACT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCCCa                                                                            >  2:15198/1‑239 (MQ=255)

                                                                                                                                                        aaaaaaag‑G‑GGAAAAATTTGGCCTTT‑AGGCC‑AAATTTTTTCCGG‑GGA‑GTCGAGAAAAAACCCGGGAA‑GAATAGCGGTAACC‑GGGG‑AAAAAAACT‑CCGCCACCAAAAAGAT‑AACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGAtt                                                                        <  2:268499/239‑1 (MQ=255)

                                                                                                                                                        aaaaaaaa‑G‑GGAAAAATTTTGCTTTT‑AAGCA‑CATTTTTTTCCCG‑CAA‑TCCAAGAAAAAACCCGAGGA‑ACTACTCGGAAAAC‑GAGG‑AAAAAATCC‑TCCCCACAAAAAAAAT‑AACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGGCGTCGCCCATACCATAGGCGATTTTTTCCCGCAGAtt                                                                        <  1:458218/239‑1 (MQ=255)

                                                                                                                                                        aaaaaaaa‑G‑GAAAGAAATTTGCCTTT‑AGCCC‑AAATATTTTCCCG‑GGA‑GTCGAGAAAAAATCCGGGGA‑CGAATTGGGTAAAC‑GGGG‑TAAAAAACT‑CCCCCCCAAGAAAGAA‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGAtt                                                                        <  2:428683/239‑1 (MQ=255)

                                                                                                                                                          aataaa‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAACT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGAAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGTCGATTTTTTCCCGCAGATTCa                                                                      >  2:229054/1‑239 (MQ=255)

                                                                                                                                                               a‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAACT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGtttt                                                                 >  2:624389/1‑239 (MQ=255)

                                                                                                                                                                    gACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAACT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATCCCATAGGCGATTTTTTCCCCCACATTCAGTTTTAAt                                                              >  2:1086987/1‑239 (MQ=255)

                                                                                                                                                                     tgacaatttttctgtttggccacAATTTTTTCCCG‑CGA‑GTC‑AATTAAAATCCGGGGAGGCATCCCGG‑AAAC‑GGGGATAAAAACCC‑ACACC‑CAAAAAAAATAAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGtttt                                                                 <  1:348476/215‑1 (MQ=255)

                                                                                                                                                                      cGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAACT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATTCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTACCGCCAATTCAGTTTTCATGt                                                            >  1:156052/1‑239 (MQ=255)

                                                                                                                                                                      cGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAACT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGt                                                            >  1:135602/1‑239 (MQ=255)

                                                                                                                                                                      cGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAACT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCACGCCGATTCAGTTTTAATg                                                             >  2:1083506/1‑238 (MQ=255)

                                                                                                                                                                         attttGCCTTT‑ATGCC‑CAATTTTTTCCCG‑CGT‑GCGGAGAAAAAATCCGGGAA‑TCATATCGGAAACC‑GCGG‑TAAAAACCT‑TCGCCACCAAAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAg                                                         <  2:413082/237‑1 (MQ=255)

                                                                                                                                                                              tgtttt‑GGGCC‑AAATTTTTTCCGG‑GGA‑ATCGAAAAAAAACCGGGGGA‑GAATTGGGGAAAAC‑GCGG‑TAAAAACCT‑CCGCAACAAGAAAGAA‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGAt                                                    <  2:526885/236‑1 (MQ=255)

                                                                                                                                                                              gCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAACT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCCTCCCTAATTCATCGGGATAAAATTTGAGAGGATACAGAGTCCCGAGGCAGGGAATACAATTCGAACCGCCTGCGCCCAACCCCTAGGCGTTTTTTTCCCTCAAATTCacatataacataagagaca                                                    >  1:573614/1‑221 (MQ=255)

                                                                                                                                                                               ccTTG‑AGCCA‑CAATTTTTTCCCG‑GGA‑GTCAAAAAAAAATCCGGTGA‑GCATATGGAAAAAC‑GCGA‑AAAAAACCT‑CCCCCACCAAAAAGAT‑ATCCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGAtt                                                   <  2:21196/239‑1 (MQ=255)

                                                                                                                                                                                 ttt‑AGGCCACAATTTTTTCCCG‑GAA‑GTGGAAAAAAATCCCGGTGA‑CAATACCGGAAAAC‑GCGG‑TAAAAACCT‑TCCCCACCAAAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTa                                                  <  2:1304304/239‑1 (MQ=255)

                                                                                                                                                                                     agccc‑cATTTTTTTCCCG‑GGA‑GTCAATAAAAAATCCGGGAA‑GATTATCGGTAAAC‑GGGA‑AAAAAAACT‑TCCCCCCAAAAAAAAT‑AACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGt                                              <  2:723233/236‑1 (MQ=255)

                                                                                                                                                                                     aGGCC‑TAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAACT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGCAGATATACAGAGTAACGATGCAGGGCATTAAATTCTAACCGCCGTGGCCCATACCATGGGGGCTTTTTTCCCCGAAATTCCGTTTTAACGTCAAGTGATTATgtg                                              >  1:750390/1‑237 (MQ=255)

                                                                                                                                                                                     aGGCC‑AAATTTTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGGAA‑GAATTCCGGTAAAC‑GCGA‑TAAAAACCT‑TCCCCACCAAAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGt                                              <  2:651645/239‑1 (MQ=255)

                                                                                                                                                                                        aacaaaTTTTTTCCCG‑GGA‑GTGGAGAAAAAGTCGGGGGA‑GAATATCGGAAAAC‑GGGG‑TAAAAACCT‑CCACCACAAGAAAGAA‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCa                                            <  2:1286880/235‑1 (MQ=255)

                                                                                                                                                                                                      cccgagagcggagtAAAAGTCCGGTGA‑GCATACCGGTAAAC‑GGGG‑TAAAAACCT‑TCACCACCAAAAAAAT‑AACTCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACt                                <  2:1179303/229‑1 (MQ=255)

                                                                                                                                                                                                         cga‑gtcgagtAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAACT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATATTCACTCCtt                            >  2:820610/1‑239 (MQ=255)

                                                                                                                                                                                                         cga‑gtcgagtAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAACT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGCCGATTTTTTCCCGCAGATTACGTTTTAATGTAAGTGGATTATGGTCAGACATAATCAcctt                              >  1:1286880/1‑234 (MQ=255)

                                                                                                                                                                                                                       actCCGGTGA‑GCATATCGGAAACC‑GGGG‑AAAAAACCT‑TCGCCACCAAAAAAAA‑AACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCa               <  2:299098/237‑1 (MQ=255)

                                                                                                                                                                                                                            ggTGA‑GTATATCGTAAAAC‑AGGT‑TAAAAACCG‑TCGCCCCCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGcg          <  2:349800/239‑1 (MQ=255)

                                                                                                                                                                                                                              tGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAACT‑TCGCCCCCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAAtt                                                                                                                                                          <  2:806756/93‑1 (MQ=255)

                                                                                                                                                                                                                              tGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAACT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAAtt                                                                                                                                                          >  1:806756/1‑93 (MQ=255)

                                                                                                                                                                                                                                     tgccGGAAAAC‑GCGA‑AAAAAACCT‑CCCCAAGAAGAAAAAG‑AACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTttat  <  1:1235587/236‑1 (MQ=255)

                                                                                                                                                                                                                                      atCGGGAAACGGAGG‑AAAAAACCT‑TCGCCACAAAAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTttat  <  2:535661/239‑1 (MQ=255)

ATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACA‑GCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCG‑‑ATAAACTGGAGCG‑TTGCAATTAACGCCGC‑CGGAACCGCTGCATATAAAATAAA‑G‑GGACGAAATTTGCCTTT‑AGGCC‑CAATATTTTTCCG‑CGA‑GTCGAGTAAAAATCCGGTGA‑GCATATCGGTAAAC‑GCGG‑TAAAAAACT‑TCGCCACCAGAAAGAT‑AATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTTTAT  >  NC_000913/4065347‑4065802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A