breseq  version 0.32.0  revision 6ff6de7d1b87
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 37,250 A→C 18.6% V192G (GTG→GGG)  caiC ← putative crotonobetaine/carnitine‑CoA ligase
RA 37,255 A→C 17.3% G190G (GGT→GGG caiC ← putative crotonobetaine/carnitine‑CoA ligase
RA 71,870 A→G 17.7% S174G (AGC→GGC)  yabI → DedA family inner membrane protein
RA 82,217 C→G 9.4% R438P (CGC→CCC)  leuA ← 2‑isopropylmalate synthase
RA 83,780 C→A 11.5% intergenic (‑72/‑588) leuL ← / → leuO leu operon leader peptide/global transcription factor
RA 86,765 A→G 10.9% E379G (GAG→GGG)  ilvI → acetolactate synthase 3 large subunit
RA 86,769 T→C 6.7% T380T (ACT→ACC ilvI → acetolactate synthase 3 large subunit
RA 99,078 A→T 12.6% I226F (ATC→TTC)  ftsW → putative lipid II flippase, integral membrane protein, FtsZ ring stabilizer
RA 154,321 A→C 11.3% V369G (GTC→GGC)  htrE ← putative outer membrane usher protein
RA 164,031 T→G 8.8% W643G (TGG→GGG)  hrpB → putative ATP‑dependent helicase
RA 175,537 T→G 22.7% V144G (GTG→GGG)  clcA → H(+)/Cl(‑) exchange transporter
RA 218,734 T→G 8.6% T14T (ACA→ACC proS ← prolyl‑tRNA synthetase
RA 252,387 G→A 13.0% K29K (AAG→AAA yafO → mRNA interferase toxin of the YafO‑YafN toxin‑antitoxin system
MC JC 257,908 Δ776 bp 100% [crl] [crl]
RA 319,100 T→A 5.0% Y301F (TAC→TTC)  rclA ← reactive chlorine stress species (RCS) resistance protein, pyridine nucleotide‑dependent disulfide oxidoreductase family
RA 343,444 A→G 14.5% G187G (GGA→GGG yahK → broad specificity NADPH‑dependent aldehyde reductase, Zn‑containing
RA 345,302 C→A 17.2% intergenic (+311/‑102) yahL → / → yahM uncharacterized protein/uncharacterized protein
RA 361,668 C→T 17.0% E65K (GAA→AAA)  lacA ← thiogalactoside acetyltransferase
RA 361,728 C→T 16.9% E45K (GAA→AAA)  lacA ← thiogalactoside acetyltransferase
RA 388,772 T→C 10.2% E319G (GAG→GGG)  hemB ← 5‑aminolevulinate dehydratase (porphobilinogen synthase)
RA 417,533 T→G 19.2% V131G (GTG→GGG)  phoB → response regulator in two‑component regulatory system with PhoR
RA 429,830 T→G 19.0% V109G (GTG→GGG)  secF → SecYEG protein translocase auxillary subunit
RA 433,649 T→G 39.7% G65G (GGT→GGG ribD → fused diaminohydroxyphosphoribosylaminopyrimidine deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase
RA 437,444 A→C 18.8% V231G (GTG→GGG)  yajO ← 2‑carboxybenzaldehyde reductase
RA 442,372 T→G 12.6% V275G (GTG→GGG)  thiI → tRNA s(4)U8 sulfurtransferase
RA 452,547 G→C 14.9% G333G (GGC→GGG ampG ← muropeptide transporter
RA 454,195 T→G 12.0% intergenic (‑28/‑277) yajG ← / → bolA putative lipoprotein/stationary‑phase morphogene, transcriptional repressor for mreB, also regulator for dacA, dacC, and ampC
RA 465,928 C→T 4.9% R462K (AGA→AAA)  ybaE ← putative ABC transporter periplasmic binding protein
RA 484,581 A→C 9.8% W347G (TGG→GGG)  acrA ← multidrug efflux system
RA 538,368 A→G 13.6% S246G (AGC→GGC)  ybbW → putative allantoin transporter
RA 540,577 G→T 15.1% A5S (GCA→TCA)  ybbY → putative uracil/xanthine transporter
RA 569,209 T→C 3.6% L103S (TTG→TCG)  ybcK → DLP12 prophage, putative recombinase
RA 649,330 A→C 11.5% V81G (GTG→GGG)  citF ← citrate lyase, citrate‑ACP transferase (alpha) subunit
RA 665,104 A→C 18.9% S29R (AGT→AGG rlpA ← septal ring protein, suppressor of prc, minor lipoprotein
RA 715,338 T→A 11.1% intergenic (+140/‑74) pgm → / → ybfP phosphoglucomutase/lipoprotein
RA 727,561 A→C 16.8% V391G (GTG→GGG)  kdpA ← potassium translocating ATPase, subunit A
RA 759,427 A→G 13.9% E241G (GAG→GGG)  sucA → 2‑oxoglutarate decarboxylase, thiamine triphosphate‑binding
RA 770,523 A→C 11.0% H849P (CAC→CCC)  mngB → alpha‑mannosidase
RA 783,293 A→G 16.1% E43G (GAG→GGG)  pnuC → nicotinamide riboside transporter
RA 811,721 T→G 24.7% V67G (GTG→GGG)  bioC → malonyl‑ACP O‑methyltransferase, SAM‑dependent
RA 825,248 G→C 20.2% G287G (GGC→GGG ybhR ← putative ABC transporter permease
RA 846,304 T→G 11.6% T54P (ACC→CCC)  glnQ ← glutamine transporter subunit
RA 861,108 A→C 16.7% V500G (GTG→GGG)  ybiW ← putative pyruvate formate lyase
RA 871,360 A→G 17.9% S132G (AGT→GGT)  gsiC → glutathione ABC transporter permease
RA 909,181 A→G 13.9% A38A (GCT→GCC ltaE ← L‑allo‑threonine aldolase, PLP‑dependent
RA 956,640 A→C 15.8% intergenic (‑8/‑122) ycaO ← / → ycaP ribosomal protein S12 methylthiotransferase accessory factor/UPF0702 family putative inner membrane protein
RA 973,698 A→G 14.8% G54G (GGA→GGG smtA → putative S‑adenosyl‑L‑methionine‑dependent methyltransferase
RA 1,058,486 T→C 21.7% S135P (TCG→CCG)  torC → trimethylamine N‑oxide (TMAO) reductase I, cytochrome c‑type subunit
RA 1,069,065 G→A 14.7% L259L (CTG→TTG)  rutG ← pyrimidine permease
RA 1,123,777 A→C 12.4% V93G (GTC→GGC)  grxB ← glutaredoxin 2 (Grx2)
RA 1,137,894 T→C 16.0% L175P (CTC→CCC)  flgJ → flagellar rod assembly protein and murein hydrolase, flagellum‑specific muramidase
RA 1,158,048 A→C 20.7% P60P (CCA→CCC ptsG → fused glucose‑specific PTS enzymes: IIB component/IIC component
RA 1,196,305 C→T 89.0% R395C (CGT→TGT)  icd → isocitrate dehydrogenase, e14 prophage attachment site, tellurite reductase
RA 1,263,180 G→T 19.3% D107E (GAC→GAA lolB ← lipoprotein localization factor
RA 1,271,857 A→T 16.5% intergenic (‑8/‑262) chaA ← / → chaB calcium/sodium:proton antiporter/cation transport regulator
RA 1,272,501 T→G 16.1% intergenic (+152/‑6) chaB → / → chaC cation transport regulator/cation transport regulator
RA 1,357,427 T→C 9.2% E81G (GAG→GGG)  ymjA ← DUF2543 family protein
RA 1,383,219 A→C 12.4% V248G (GTG→GGG)  ycjW ← LacI family putative transcriptional repressor
RA 1,383,718 C→A 19.6% E82* (GAA→TAA)  ycjW ← LacI family putative transcriptional repressor
RA 1,404,865 A→G 16.1% E42G (GAG→GGG)  abgR → putative DNA‑binding transcriptional regulator of abgABT operon
RA 1,435,725 C→A 18.0% intergenic (‑106/+35) uspF ← / ← ompN stress‑induced protein, ATP‑binding protein/outer membrane pore protein N, non‑specific
RA 1,464,046 G→T 12.8% E170* (GAA→TAA)  paaX → transcriptional repressor of phenylacetic acid degradation paa operon, phenylacetyl‑CoA inducer
RA 1,466,210 G→A 6.8% pseudogene (819/2513 nt) ydbA → pseudogene, autotransporter homolog, interrupted by IS2 and IS30
RA 1,473,746 T→G 21.5% pseudogene (3230/3497 nt) ydbA → pseudogene, autotransporter homolog, interrupted by IS2 and IS30
RA 1,477,207 A→C 16.4% A688A (GCA→GCC ydbD → PF10971 family putative periplasmic methylglyoxal resistance protein
RA 1,503,645 Δ1 bp 3.6% pseudogene (5/432 nt) insP ← pseudogene, IS609 transposase A,IS, phage, Tn, Transposon‑related functions, extrachromosomal, transposon related
RA 1,520,062 T→A 15.5% *346K (TAA→AAA)  curA → curcumin/dihydrocurcumin reductase, NADPH‑dependent
RA 1,589,069 C→T 23.4% pseudogene (1011/1227 nt) ydeT ← pseudogene, putative outer membrane protein
RA 1,616,835 T→A 16.5% I358K (ATA→AAA)  yneK → uncharacterized protein
RA 1,639,706 A→C 12.5% C118G (TGT→GGT)  rrrQ ← Qin prophage, putative lysozyme
RA 1,744,088 A→T 12.6% Y211F (TAC→TTC)  mdtK → multidrug efflux system transporter
RA 1,827,565 A→C 11.8% V232G (GTG→GGG)  astB ← succinylarginine dihydrolase
RA 1,872,402 T→G 12.0% V121G (GTG→GGG)  yeaJ → putative diguanylate cyclase
RA 1,912,136 A→C 19.5% G147G (GGT→GGG htpX ← putative endopeptidase
RA 1,915,574 G→A 4.8% intergenic (‑40/+57) proQ ← / ← msrC RNA chaperone, putative ProP translation regulator/free methionine‑(R)‑sulfoxide reductase
RA 1,921,260 T→G 12.2% G346G (GGT→GGG rsmF → 16S rRNA m(5)C1407 methyltransferase, SAM‑dependent
RA 1,923,359 A→T 9.6% intergenic (+45/+6) ryeA → / ← yebY novel sRNA, function unknown/DUF2511 family protein
RA 1,976,685 T→C 15.9% E152G (GAG→GGG)  motA ← proton conductor component of flagella motor
RA 2,054,696 C→T 5.9% L352L (CTG→TTG)  shiA → shikimate transporter
RA 2,064,843 A→C 12.6% V127G (GTG→GGG)  cobS ← cobalamin synthase
RA 2,074,021 A→C 13.3% Y828S (TAC→TCC)  flu → CP4‑44 prophage, antigen 43 (Ag43) phase‑variable biofilm formation autotransporter
RA 2,077,484 T→C 11.0% intergenic (+4/‑85) cbeA → / → cbtA CP4‑44 prophage, cytoskeleton bundling‑enhancing factor A, CbtA antitoxin/CP4‑44 prophage, toxin of the YeeV‑YeeU toxin‑antitoxin system
RA 2,120,560 A→C 10.8% V332G (GTG→GGG)  wcaJ ← colanic biosynthesis UDP‑glucose lipid carrier transferase
RA 2,132,787 A→C 100% I204S (ATC→AGC)  wcaA ← putative glycosyl transferase
RA 2,145,868 A→G 10.6% E868G (GAG→GGG)  yegE → putative diguanylate cyclase
RA 2,193,508 A→C 9.9% V220G (GTG→GGG)  mrp ← antiporter inner membrane protein
RA 2,219,304 A→C 15.5% V59G (GTG→GGG)  osmF ← putative ABC transporter periplasmic binding protein
RA 2,235,396 T→G 23.1% V45G (GTG→GGG)  preA → dihydropyrimidine dehydrogenase, NADH‑dependent, subunit C
RA 2,274,347 T→G 24.0% G57G (GGT→GGG yejB → microcin C ABC transporter permease
RA 2,331,257 A→C 11.1% pseudogene (3144/4605 nt) yfaS ← pseudogene, bacterial alpha2‑macroglobulin YfaS variant family, putative membrane protein
RA 2,349,403 T→C 8.6% E23G (GAG→GGG)  inaA ← acid‑inducible Kdo/WaaP family putative kinase
RA 2,459,223 T→C 25.4% G415G (GGA→GGG fadI ← beta‑ketoacyl‑CoA thiolase, anaerobic, subunit
RA 2,504,890 A→C 16.7% V219G (GTG→GGG)  ypdE ← aminopeptidase
RA 2,513,127 A→T 9.1% I30F (ATC→TTC)  nupC → nucleoside (except guanosine) transporter
RA 2,541,268 T→C 18.2% E137G (GAG→GGG)  cysW ← sulfate/thiosulfate ABC transporter permease
RA 2,623,865 T→G 11.6% V275G (GTG→GGG)  ppk → polyphosphate kinase, component of RNA degradosome
RA 2,625,567 T→G 11.9% V152G (GTG→GGG)  ppx → exopolyphosphatase
RA 2,649,242 A→C 18.6% V1015G (GTG→GGG)  yfhM ← bacterial alpha2‑macroglobulin colonization factor ECAM, anti‑host protease defense factor, periplasmic inner membrane‑anchored lipoprotein
RA 2,704,839 T→G 11.6% T157P (ACT→CCT)  lepB ← leader peptidase (signal peptidase I)
RA 2,707,558 Δ1 bp 100% coding (242/480 nt) rseC ← SoxR iron‑sulfur cluster reduction factor component
RA 2,712,526 A→C 10.0% S79R (AGT→AGG trmN ← tRNA1(Val) (adenine(37)‑N6)‑methyltransferase
RA 2,739,682 T→C 13.9% E129G (GAG→GGG)  tyrA ← fused chorismate mutase T/prephenate dehydrogenase
RA 2,745,087 A→C 10.0% V84G (GTG→GGG)  trmD ← tRNA m(1)G37 methyltransferase, SAM‑dependent
RA 2,777,457 A→C 15.3% T3P (ACT→CCT)  ypjF → CP4‑57 prophage, toxin of the YpjF‑YfjZ toxin‑antitoxin system
RA 2,872,178 A→C 13.6% V104G (GTG→GGG)  ispD ← 4‑diphosphocytidyl‑2C‑methyl‑D‑erythritol synthase
RA 2,877,025 T→G 16.8% G149G (GGT→GGG iap → aminopeptidase in alkaline phosphatase isozyme conversion
RA 2,909,227 A→C 13.1% G146G (GGT→GGG pyrG ← CTP synthetase
RA 2,913,657 T→C 12.7% intergenic (‑8/+40) relA ← / ← rlmD (p)ppGpp synthetase I/GTP pyrophosphokinase/23S rRNA m(5)U1939 methyltransferase, SAM‑dependent
RA 2,944,709 A→G 11.4% E57G (GAG→GGG)  csdE → CsdA‑binding activator, Fe‑S protein
RA 2,947,215 G→A 11.7% intergenic (‑39/‑170) mltA ← / → metZ membrane‑bound lytic murein transglycosylase A/tRNA‑Met
RA 2,952,348 C→G 12.3% A38P (GCG→CCG)  recD ← exonuclease V (RecBCD complex), alpha chain
RA 2,967,843 A→C 17.8% V197G (GTG→GGG)  ptsP ← PEP‑protein phosphotransferase enzyme I, GAF domain containing protein
RA 3,017,755 T→G 14.8% G566G (GGT→GGG ygfK → putative Fe‑S subunit oxidoreductase subunit
RA 3,051,837 A→C 12.3% V160G (GTG→GGG)  ubiI ← 2‑octaprenylphenol hydroxylase, FAD‑dependent
RA 3,053,470 G→C 16.9% A16G (GCG→GGG)  ubiH ← 2‑octaprenyl‑6‑methoxyphenol hydroxylase, FAD/NAD(P)‑binding
RA 3,062,716 A→C 14.2% T623T (ACA→ACC scpA → methylmalonyl‑CoA mutase
RA 3,074,200 T→C 12.0% E163G (GAG→GGG)  yggC ← putative PanK family P‑loop kinase
RA 3,090,487 G→T 11.6% C48F (TGC→TTC)  endA → DNA‑specific endonuclease I
RA 3,135,444 G→T 11.2% A309E (GCA→GAA)  pitB ← phosphate transporter
RA 3,140,309 T→C 13.3% E3G (GAG→GGG) 
*163W (TGA→TGG
hybF ←
hybE ←
protein involved with the maturation of hydrogenases 1 and 2
hydrogenase 2‑specific chaperone
RA 3,141,135 G→C 15.2% G50G (GGC→GGG hybD ← maturation protease for hydrogenase 2
JC 3,149,291:1 (CCA)2→3 4.2% coding (179/495 nt) yqhA ← UPF0114 family putative inner membrane protein
RA 3,206,436 G→A 17.6% intergenic (‑182/‑25) ttdR ← / → ttdA transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
RA 3,206,450 C→A 16.3% intergenic (‑196/‑11) ttdR ← / → ttdA transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
RA 3,206,469 C→G 16.1% S3R (AGC→AGG ttdA → L‑tartrate dehydratase, alpha subunit
RA 3,206,475 T→A 16.5% S5R (AGT→AGA ttdA → L‑tartrate dehydratase, alpha subunit
RA 3,206,481 G→A 16.4% K7K (AAG→AAA ttdA → L‑tartrate dehydratase, alpha subunit
RA 3,206,504 C→A 16.5% T15K (ACA→AAA)  ttdA → L‑tartrate dehydratase, alpha subunit
RA 3,209,178 T→G 13.9% V386G (GTG→GGG)  ttdT → L‑tartrate/succinate antiporte
RA 3,209,978 A→G 20.4% A188A (GCT→GCC tsaD ← tRNA(ANN) t(6)A37 threonylcarbamoyladenosine modification protein, glycation binding protein
RA 3,218,574 T→C 14.2% G167G (GGA→GGG aer ← fused signal transducer for aerotaxis sensory component/methyl accepting chemotaxis component
RA 3,263,923 T→G 11.7% T365P (ACT→CCT)  tdcC ← L‑threonine/L‑serine transporter
RA 3,265,358 T→C 18.5% G223G (GGA→GGG tdcB ← L‑threonine dehydratase, catabolic
RA 3,282,197 T→C 13.4% L75P (CTC→CCC)  agaS → tagatose‑6‑phosphate ketose/aldose isomerase
RA 3,289,076 C→A 15.5% Q89K (CAG→AAG)  yraJ → putative outer membrane protein
RA 3,308,006 C→A 9.8% intergenic (‑75/+32) yrbN ← / ← nlpI uncharacterized protein/lipoprotein involved in osmotic sensitivity and filamentation
RA 3,317,601 A→C 12.3% V135G (GTG→GGG)  rimP ← ribosome maturation factor for 30S subunits
RA 3,319,871 A→C 10.4% T413P (ACT→CCT)  argG → argininosuccinate synthetase
RA 3,332,559 T→C 17.2% R59G (AGG→GGG)  yhbE ← EamA family inner membrane putative transporter
RA 3,338,867 T→C 12.5% E127G (GAG→GGG)  mlaE ← ABC transporter maintaining OM lipid asymmetry, inner membrane permease protein
RA 3,340,103 G→C 11.0% intergenic (‑40/‑170) mlaF ← / → yrbG ABC transporter maintaining OM lipid asymmetry, ATP‑binding protein/putative calcium/sodium:proton antiporter
RA 3,344,982 A→C 15.0% T90P (ACC→CCC)  rpoN → RNA polymerase, sigma 54 (sigma N) factor
RA 3,364,405 C→A 11.6% S534* (TCA→TAA)  yhcD → putative outer membrane fimbrial subunit usher protein
RA 3,395,439 T→C 11.8% E273G (GAG→GGG)  yhdP ← DUF3971‑AsmA2 domains protein
RA 3,405,779 T→G 12.9% C116G (TGC→GGC)  accB → acetyl CoA carboxylase, BCCP subunit
RA 3,416,432 C→T 16.1% R468C (CGC→TGC)  acrF → multidrug efflux system protein
RA 3,417,904 T→G 12.4% G958G (GGT→GGG acrF → multidrug efflux system protein
RA 3,428,569 T→C 11.5% noncoding (192/1542 nt) rrsD ← 16S ribosomal RNA of rrnD operon
RA 3,438,595 G→T 6.1% A18E (GCA→GAA)  arfA ← alternate ribosome‑rescue factor A
RA 3,439,037 A→C 12.1% V31G (GTG→GGG)  zntR ← zntA gene transcriptional activator
RA 3,460,785 T→C 17.4% F324S (TTC→TCC)  gspF → general secretory pathway component, cryptic
RA 3,473,919 G→A 9.2% S54F (TCT→TTT)  rpsG ← 30S ribosomal subunit protein S7
RA 3,479,970 G→C 13.5% G212G (GGC→GGG kefB ← potassium:proton antiporter
RA 3,480,294 T→C 20.3% G104G (GGA→GGG kefB ← potassium:proton antiporter
RA 3,492,827 G→T 13.2% A88S (GCC→TCC)  tsgA → putative transporter
RA 3,499,607 A→C 17.1% A54A (GCA→GCC yhfL → small lipoprotein
RA 3,499,649 G→T 24.0% intergenic (+36/‑259) yhfL → / → frlA small lipoprotein/putative fructoselysine transporter
RA 3,499,692 G→T 13.4% intergenic (+79/‑216) yhfL → / → frlA small lipoprotein/putative fructoselysine transporter
RA 3,499,701 G→T 11.4% intergenic (+88/‑207) yhfL → / → frlA small lipoprotein/putative fructoselysine transporter
RA 3,520,595 A→C 8.8% V36G (GTG→GGG)  hofQ ← DNA catabolic putative fimbrial transporter
RA 3,522,373 A→G 14.5% L126P (CTC→CCC)  hofM ← DNA catabolic putative pilus assembly protein
RA 3,532,593 C→A 13.8% intergenic (‑156/‑223) yhgE ← / → pck DUF4153 family putative inner membrane protein/phosphoenolpyruvate carboxykinase [ATP]
RA 3,536,133 G→C 23.1% R150R (CGC→CGG ompR ← response regulator in two‑component regulatory system with EnvZ
RA 3,551,915 A→C 15.9% V186G (GTG→GGG)  malP ← maltodextrin phosphorylase
RA 3,553,283 T→G 17.0% G67G (GGT→GGG malT → mal regulon transcriptional activator
RA 3,590,223 T→G 13.2% T230P (ACC→CCC)  ugpA ← sn‑glycerol‑3‑phosphate ABC transporter permease
RA 3,592,949 T→C 4.4% E163G (GAG→GGG)  livF ← branched‑chain amino acid ABC transporter ATPase
RA 3,622,502 T→G 14.9% S1104R (AGT→AGG rhsB → Rhs protein with DUF4329 family putative toxin domain, putative neighboring cell growth inhibitor
RA 3,638,184 A→G 17.2% S182G (AGC→GGC)  pitA → phosphate transporter, low‑affinity, tellurite importer
RA 3,638,227 A→G 17.6% E196G (GAG→GGG)  pitA → phosphate transporter, low‑affinity, tellurite importer
RA 3,640,983 C→T 14.7% F41F (TTC→TTT dtpB → dipeptide and tripeptide permease B
RA 3,665,587 C→A 9.3% A75S (GCA→TCA)  gadX ← acid resistance regulon transcriptional activator, autoactivator
RA 3,672,788 A→G 6.3% S126G (AGT→GGT)  yhjC → LysR family putative transcriptional regulator
RA 3,673,736 A→C 19.5% T126P (ACC→CCC)  yhjD → inner membrane putative BrbK family alternate lipid exporter
RA 3,674,171 A→T 6.4% I271F (ATT→TTT)  yhjD → inner membrane putative BrbK family alternate lipid exporter
RA 3,686,538 T→C 15.2% S879G (AGC→GGC)  bcsC ← cellulose synthase subunit
RA 3,699,211 G→T 14.7% L333F (TTG→TTT bcsG → DUF3260 family cellulose production inner membrane protein
RA 3,731,743 T→A 11.9% L205Q (CTA→CAA)  xylF → D‑xylose transporter subunit
RA 3,741,499 T→C 8.5% E28G (GAG→GGG)  ysaA ← putative hydrogenase, 4Fe‑4S ferredoxin‑type component
RA 3,749,428 T→G 19.7% G66G (GGT→GGG sgbU → putative L‑xylulose 5‑phosphate 3‑epimerase
RA 3,762,268 T→G 22.8% G29G (GGT→GGG rhsA → Rhs protein with putative toxin 55 domain, putative polysaccharide synthesis/export protein, putative neighboring cell growth inhibitor
RA 3,765,493 T→G 20.9% S1104R (AGT→AGG rhsA → Rhs protein with putative toxin 55 domain, putative polysaccharide synthesis/export protein, putative neighboring cell growth inhibitor
RA 3,771,322 G→A 10.7% I19I (ATC→ATT yibH ← putative membrane fusion protein (MFP) component of efflux pump
RA 3,777,790 A→G 9.8% G131G (GGA→GGG lldP → L‑lactate permease
RA 3,784,899 A→C 13.3% V39G (GTG→GGG)  yibN ← putative rhodanese‑related sulfurtransferase
MC JC 3,815,858 Δ82 bp 100% [rph][rph] [rph], [rph]
RA 3,840,797 G→A 9.2% I314I (ATC→ATT nepI ← putative transporter
RA 3,852,083 T→C 10.2% E234G (GAG→GGG)  ilvB ← acetolactate synthase 2 large subunit
RA 3,854,897 A→G 18.4% E326G (GAG→GGG)  emrD → multidrug efflux system protein
RA 3,862,661 T→G 30.2% pseudogene (942/1617 nt) glvC ← pseudogene, arbutin specific enzyme IIC component of PTS,enzyme, Transport of small molecules: Carbohydrates, organic acids, alcohols, PTS system, arbutin‑like IIB component, PTS system, arbutin‑like IIC component
RA 3,862,673 C→A 30.8% pseudogene (930/1617 nt) glvC ← pseudogene, arbutin specific enzyme IIC component of PTS,enzyme, Transport of small molecules: Carbohydrates, organic acids, alcohols, PTS system, arbutin‑like IIB component, PTS system, arbutin‑like IIC component
RA 3,862,676 G→C 32.7% pseudogene (927/1617 nt) glvC ← pseudogene, arbutin specific enzyme IIC component of PTS,enzyme, Transport of small molecules: Carbohydrates, organic acids, alcohols, PTS system, arbutin‑like IIB component, PTS system, arbutin‑like IIC component
RA 3,880,847 A→G 9.6% L125P (CTC→CCC)  recF ← gap repair protein
RA 3,901,864 A→C 9.8% V119G (GTG→GGG)  bglH ← carbohydrate‑specific outer membrane porin, cryptic
RA 3,915,874 A→C 9.9% L33R (CTG→CGG)  atpC ← F1 sector of membrane‑bound ATP synthase, epsilon subunit
RA 3,932,711 A→G 11.2% E466G (GAG→GGG)  kup → potassium transporter
RA 3,943,487 T→G 13.1% noncoding (54/76 nt) gltU → tRNA‑Glu
RA 3,956,960 T→C 8.9% E287G (GAG→GGG)  ilvY ← transcriptional activator of ilvC, autorepressor
RA 3,975,376 A→G 22.4% S78G (AGC→GGC)  wecE → TDP‑4‑oxo‑6‑deoxy‑D‑glucose transaminase
RA 3,975,382 A→C 17.4% T80P (ACC→CCC)  wecE → TDP‑4‑oxo‑6‑deoxy‑D‑glucose transaminase
RA 3,977,198 A→G 16.5% S308G (AGT→GGT)  wzxE → O‑antigen translocase
RA 3,981,334 A→G 13.9% E150G (GAG→GGG)  yifK → putative APC family amino acid transporter
RA 3,991,072:1 +G 2.6% intergenic (‑307/‑80) hemC ← / → cyaA hydroxymethylbilane synthase/adenylate cyclase
JC 4,001,644 Δ5 bp 96.4% coding (220‑224/951 nt) corA → magnesium/nickel/cobalt transporter
RA 4,013,576 T→G 11.7% G175G (GGT→GGG metE → 5‑methyltetrahydropteroyltriglutamate‑ homocysteine S‑methyltransferase
RA 4,043,013 A→G 13.6% G200G (GGA→GGG srkA → Cpx stress response Thr/Ser protein kinase, MazF antagonist protein
RA 4,045,671 A→C 7.9% *311E (TAA→GAA)  yihG ← inner membrane protein, inner membrane acyltransferase
RA 4,046,373 G→A 9.3% L77L (CTG→TTG)  yihG ← inner membrane protein, inner membrane acyltransferase
RA 4,062,374 T→G 8.1% G43G (GGT→GGG yihN → MFS transporter family protein
RA 4,062,444 G→T 6.6% A67S (GCC→TCC)  yihN → MFS transporter family protein
RA 4,065,547 T→A 13.9% L73F (TTA→TTT yihO ← putative sulphoquinovose importer
RA 4,065,570 T→A 16.4% T66S (ACC→TCC)  yihO ← putative sulphoquinovose importer
RA 4,065,595 C→A 15.1% L57L (CTG→CTT yihO ← putative sulphoquinovose importer
RA 4,072,424 T→C 7.8% E76G (GAG→GGG)  yihT ← 6‑deoxy‑6‑sulphofructose‑1‑phosphate aldolase
RA 4,101,254 C→T 9.5% S149F (TCT→TTT)  sodA → superoxide dismutase, Mn
RA 4,103,630 T→C 9.4% I449V (ATT→GTT)  cpxA ← sensory histidine kinase in two‑component regulatory system with CpxR
RA 4,103,632 A→C 15.1% V448G (GTG→GGG)  cpxA ← sensory histidine kinase in two‑component regulatory system with CpxR
RA 4,107,591 C→G 7.9% A14G (GCG→GGG)  pfkA → 6‑phosphofructokinase I
RA 4,135,881 T→G 10.9% V683G (GTG→GGG)  katG → catalase‑peroxidase HPI, heme b‑containing
RA 4,136,803 A→G 15.2% S233G (AGC→GGC)  yijE → EamA‑like transporter family protein
RA 4,140,197 T→G 14.8% H675P (CAC→CCC)  frwA ← putative PTS enzyme: Hpr, enzyme I and II components
RA 4,143,497 A→G 14.8% G323G (GGA→GGG frwC → putative enzyme IIC component of PTS
RA 4,158,652 T→G 11.0% V55G (GTG→GGG)  oxyR → oxidative and nitrosative stress transcriptional regulator
RA 4,184,542 C→A 100% P1100Q (CCG→CAG)  rpoB → RNA polymerase, beta subunit
RA 4,192,033 A→C 17.9% V201G (GTG→GGG)  thiG ← thiamine biosynthesis ThiGH complex subunit
RA 4,201,277 A→C 19.0% G137G (GGT→GGG zraP ← Zn‑dependent periplasmic chaperone
RA 4,217,718 T→G 11.0% V204G (GTG→GGG)  aceA → isocitrate lyase
RA 4,217,811 C→G 4.5% A235G (GCT→GGT)  aceA → isocitrate lyase
RA 4,219,538 C→T 16.6% A315V (GCG→GTG)  aceK → isocitrate dehydrogenase kinase/phosphatase
RA 4,241,926 T→A 9.5% T110S (ACC→TCC)  xylE ← D‑xylose transporter
RA 4,241,997 T→C 15.1% D86G (GAT→GGT)  xylE ← D‑xylose transporter
RA 4,253,208 T→G 11.8% V65G (GTG→GGG)  ubiA → p‑hydroxybenzoate octaprenyltransferase
RA 4,279,724 T→G 6.4% V416G (GTG→GGG)  ghxP → guanine/hypoxanthine permease, high affinity, guanine/hypoxanthine:H+ symporter
RA 4,280,456 C→T 12.7% L160L (CTG→TTG)  yjcE → putative cation/proton antiporter
RA 4,287,459 C→A 6.7% intergenic (‑89/‑304) acs ← / → nrfA acetyl‑CoA synthetase/nitrite reductase, formate‑dependent, cytochrome
RA 4,295,699 T→G 18.6% V407G (GTG→GGG)  gltP → glutamate/aspartate:proton symporter
RA 4,300,841 A→G 6.9% L64P (CTC→CCC)  mdtP ← outer membrane factor of efflux pump
RA 4,313,537 A→G 9.7% I63V (ATT→GTT)  rpiB → ribose 5‑phosphate isomerase B/allose 6‑phosphate isomerase
RA 4,317,499 A→G 15.7% L133P (CTC→CCC)  phnL ← ribophosphonate triphosphate synthase subunit, putative ABC transporter‑related ATPase
RA 4,319,405 A→C 11.5% V68G (GTG→GGG)  phnJ ← carbon‑phosphorus lyase, SAM‑dependent
RA 4,327,764 A→G 8.8% E210G (GAG→GGG)  crfC → clamp‑binding sister replication fork colocalization protein, dynamin‑related
RA 4,359,236 A→G 9.4% L266P (CTC→CCC)  cadB ← putative lysine/cadaverine transporter
RA 4,368,876 A→G 19.7% E71G (GAG→GGG)  fxsA → suppressor of F exclusion of phage T7
RA 4,368,889 T→G 17.3% S75R (AGT→AGG fxsA → suppressor of F exclusion of phage T7
RA 4,368,899 A→G 13.7% I79V (ATC→GTC)  fxsA → suppressor of F exclusion of phage T7
RA 4,368,938 A→T 11.3% T92S (ACC→TCC)  fxsA → suppressor of F exclusion of phage T7
RA 4,378,929 A→G 19.8% L6P (CTC→CCC)  ampC ← penicillin‑binding protein, beta‑lactamase, intrinsically weak
JC 4,381,476 Δ3 bp 2.6% coding (842‑844/1809 nt) frdA ← anaerobic fumarate reductase catalytic and NAD/flavoprotein subunit
RA 4,401,810 A→G 9.4% R380G (AGA→GGA)  hflX → GTPase, stimulated by 50S subunit binding, Mn(2+) homeostasis regulator
RA 4,402,980 Δ1 bp 3.0% coding (942/1260 nt) hflK → modulator for HflB protease specific for phage lambda cII repressor
RA 4,403,906 T→G 16.1% G202G (GGT→GGG hflC → HflB protease modulator specific for phage lambda cII repressor
RA 4,424,137 T→G 14.6% V146G (GTG→GGG)  ulaF → L‑ribulose 5‑phosphate 4‑epimerase
RA 4,425,175 G→A 2.8% P19P (CCG→CCA rpsF → 30S ribosomal subunit protein S6
RA 4,429,998 C→T 17.3% S45F (TCT→TTT)  cycA → D‑alanine/D‑serine/glycine transporter
RA 4,444,675 T→G 13.5% V188G (GTG→GGG)  tamB → translocation and assembly module for autotransporter export, inner membrane subunit
RA 4,455,886 T→G 13.4% V34G (GTG→GGG)  mpl → UDP‑N‑acetylmuramate:L‑alanyl‑gamma‑D‑glutamyl‑ meso‑diaminopimelate ligase
RA 4,459,472 T→G 11.6% intergenic (+160/‑19) pmbA → / → cybC putative antibiotic peptide MccB17 maturation peptidase/pseudogene, truncated cytochrome b562, cytochrome b(562)
RA 4,463,824 T→G 8.7% D296A (GAC→GCC)  treC ← trehalose‑6‑P hydrolase
RA 4,463,875 T→G 11.3% H279P (CAC→CCC)  treC ← trehalose‑6‑P hydrolase
RA 4,464,860 A→C 10.2% V441G (GTG→GGG)  treB ← trehalose‑specific PTS enzyme: IIB and IIC component
RA 4,480,112 C→T 10.4% L128F (CTC→TTC)  yjgN → DUF898 family inner membrane protein
RA 4,487,251 C→T 12.6% L345F (CTC→TTC)  lptF → lipopolysaccharide export ABC permease
RA 4,489,889 A→C 11.6% V59G (GTG→GGG)  yjgR ← DUF853 family protein with NTPase fold
RA 4,502,618 A→C 10.6% V272G (GTG→GGG)  insG ← IS4 transposase
RA 4,534,597 G→A 13.0% pseudogene (80/246 nt) yjhZ ← pseudogene, rimK paralog, C‑terminal fragment
RA 4,542,224 C→T 13.1% R63C (CGC→TGC)  fimE → tyrosine recombinase/inversion of on/off regulator of fimA
RA 4,563,969 G→T 12.3% A241E (GCA→GAA)  yjiL ← putative ATPase, activator of (R)‑hydroxyglutaryl‑CoA dehydratase
RA 4,587,543 A→G 13.0% G198G (GGA→GGG mrr → methylated adenine and cytosine restriction protein
RA 4,588,080 A→C 16.0% W263G (TGG→GGG)  yjiA ← metal‑binding GTPase
RA 4,593,918 Δ1 bp 3.1% coding (806/1362 nt) lgoT ← putative L‑galactonate:H+ symporter
RA 4,596,353 T→G 18.9% S121R (AGT→AGG lgoD → L‑galactonate oxidoreductase, L‑gulonate oxidoreductase
RA 4,601,682 T→A 11.1% L139F (TTA→TTT yjjB ← DUF3815 family inner membrane protein
RA 4,625,086 T→G 12.7% V64G (GTG→GGG)  serB → 3‑phosphoserine phosphatase
RA 4,631,506 A→G 10.6% E258G (GAG→GGG)  slt → lytic murein transglycosylase, soluble

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 299949 300198 250 27 [26] [24] 27 paoC PaoABC aldehyde oxidoreductase, Moco‑containing subunit
* * ÷ NC_000913 583141 583757 617 27 [26] [26] 27 [appY] [appY]
* * ÷ NC_000913 1212024 1212575 552 38 [26] [21] 30 iraM/ycgX RpoS stabilzer during Mg starvation, anti‑RssB factor/DUF1398 family protein
* * ÷ NC_000913 1812686 1812984 299 27 [23] [25] 27 ydjO uncharacterized protein
* * ÷ NC_000913 1978495 1979278 784 27 [26] [6] 29 insB1–insA insB1, insA
* * ÷ NC_000913 2455622 2456120 499 27 [26] [19] 27 [yfcV] [yfcV]
* * ÷ NC_000913 2469137 2469788 652 37 [23] [26] 28 gtrS serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
* * ÷ NC_000913 3653651 3653938 288 27 [25] [26] 29 [yhiS] [yhiS]
* * ÷ NC_000913 3798957 3799506 550 28 [26] [13] 27 [waaU]–[waaZ] [waaU], [waaZ]
* * ÷ NC_000913 4233760 4235474 1715 27 [26] [26] 27 [pgi] [pgi]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 236931 =108 (1.030)80 (0.970)
+45 bp
4/342 NT 46.6% noncoding (1/77 nt) aspV tRNA‑Asp
?NC_000913 2726060 = 124 (1.180)intergenic (‑316/+7) kgtP/rrfG alpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon
* ? NC_000913 257908 =NA (NA)109 (1.040) 68/432 NT 98.2% intergenic (+9/‑768) crl/crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator,regulator, Surface structures, transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator,regulator, Surface structures, transcriptional regulator of cryptic csgA gene for curli surface fibers
?NC_000913 = 1293039 2 (0.020)intergenic (‑117/‑488) hns/tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
* ? NC_000913 279163 =103 (0.980)67 (0.640) 3/432 NT 43.5% intergenic (‑2/+15) afuB/insB1 pseudogene, CP4‑6 prophage, ferric iron ABC transporter permease gene fragment,Phage or Prophage Related/IS1 transposase B
?NC_000913 = 2760084 71 (0.680)coding (1102/1410 nt) yfjI CP4‑57 prophage, uncharacterized protein
* ? NC_000913 458787 =29 (0.280)75 (0.730) 47/426 NT 72.4% intergenic (+87/‑101) clpX/lon ATPase and specificity subunit of ClpX‑ClpP ATP‑dependent serine protease/DNA‑binding ATP‑dependent protease La
?NC_000913 608011 = NA (NA)intergenic (+23/‑54) hokE/insL1 toxic polypeptide, small/IS186 transposase
* ? NC_000913 = 45879920 (0.190)82 (0.800) 48/424 NT 80.7% intergenic (+99/‑89) clpX/lon ATPase and specificity subunit of ClpX‑ClpP ATP‑dependent serine protease/DNA‑binding ATP‑dependent protease La
?NC_000913 = 609347 NA (NA)intergenic (+170/+112) insL1/entD IS186 transposase/phosphopantetheinyltransferase component of enterobactin synthase multienzyme complex
* ? NC_000913 1207790 =32 (0.310)77 (0.790) 54/400 NT 77.1% coding (290/630 nt) stfP e14 prophage, uncharacterized protein
?NC_000913 1209619 = 16 (0.170)pseudogene (1/501 nt) stfE pseudogene, e14 prophage, side tail fiber protein fragment family,Phage or Prophage Related
* ? NC_000913 = 120780532 (0.310)65 (0.670) 42/400 NT 74.0% coding (305/630 nt) stfP e14 prophage, uncharacterized protein
?NC_000913 = 1209602 16 (0.170)pseudogene (18/501 nt) stfE pseudogene, e14 prophage, side tail fiber protein fragment family,Phage or Prophage Related
* ? NC_000913 1293032 =0 (0.000)88 (0.840) 52/432 NT 100% intergenic (‑110/‑495) hns/tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
?NC_000913 = 1979270 NA (NA)intergenic (‑55/‑483) insA/uspC IS1 repressor TnpA/universal stress protein
* ? NC_000913 = 12994980 (0.000)26 (0.250) 21/424 NT 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 1936520 =80 (0.760)11 (0.110) 10/432 NT 12.1% intergenic (‑206/‑132) zwf/yebK glucose‑6‑phosphate 1‑dehydrogenase/putative DNA‑binding transcriptional regulator
?NC_000913 = 2290111 NA (NA)intergenic (‑32/+1) insH1/yejO IS5 transposase and trans‑activator/pseudogene, autotransporter outer membrane homology,putative transport, Not classified, putative ATP‑binding component of a transport system
* ? NC_000913 = 193652481 (0.770)14 (0.130) 12/430 NT 14.8% intergenic (‑210/‑128) zwf/yebK glucose‑6‑phosphate 1‑dehydrogenase/putative DNA‑binding transcriptional regulator
?NC_000913 2101750 = NA (NA)intergenic (‑6/+145) wbbL/insH1 pseudogene, lipopolysaccharide biosynthesis protein/IS5 transposase and trans‑activator
* ? NC_000913 = 19785020 (0.000)23 (0.230) 19/416 NT 100% intergenic (‑305/+16) flhD/insB1 flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B
?NC_000913 1979279 = 0 (0.000)intergenic (‑64/‑474) insA/uspC IS1 repressor TnpA/universal stress protein
* ? NC_000913 1979486 =0 (0.000)37 (0.350) 26/432 NT 100% intergenic (‑271/‑267) insA/uspC IS1 repressor TnpA/universal stress protein
?NC_000913 2101749 = NA (NA)intergenic (‑5/+146) wbbL/insH1 pseudogene, lipopolysaccharide biosynthesis protein/IS5 transposase and trans‑activator
* ? NC_000913 = 19794890 (0.000)98 (0.940) 58/432 NT 100% intergenic (‑274/‑264) insA/uspC IS1 repressor TnpA/universal stress protein
?NC_000913 = 2290111 NA (NA)intergenic (‑32/+1) insH1/yejO IS5 transposase and trans‑activator/pseudogene, autotransporter outer membrane homology,putative transport, Not classified, putative ATP‑binding component of a transport system
* ? NC_000913 3375664 =78 (0.750)2 (0.020) 3/416 NT 2.6% coding (798/1368 nt) dcuD putative transporter
?NC_000913 3375684 = 76 (0.750)coding (818/1368 nt) dcuD putative transporter
* ? NC_000913 = 339005567 (0.640)3 (0.030) 3/426 NT 3.0% coding (538/930 nt) aaeR transcriptional regulator for aaeXAB operon
?NC_000913 = 3392499 129 (1.250)coding (3758/3801 nt) yhdP DUF3971‑AsmA2 domains protein