Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F17 I0 R1 160 98.0 2214160 89.0% 1970602 222.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,175,770 T→C 11.1% L115P (CTC→CCC)  lolC → lipoprotein‑releasing system transmembrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,175,7700TC11.1% 170.6 / 2.5 73L115P (CTC→CCC) lolClipoprotein‑releasing system transmembrane protein
Reads supporting (aligned to +/- strand):  ref base T (26/35);  new base C (6/3);  total (35/38)
Fisher's exact test for biased strand distribution p-value = 2.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAG‑CGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTC‑TTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGC‑GTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACA‑GCTAGGCGTTAATCGCGGTGATCAAATCCGCGTG‑ATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCC  >  NC_000913/1175532‑1175999
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                       
cgcGGGGTTGTGGCGCTTGGCACAGTATTGTCAGTGATGAACGGCTTTGAG‑CGCGATCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTC‑GTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGC‑GTGGCGGTCGGGGTGATGCt                                                                                                                                                                                                                                         <  1:438873/237‑1 (MQ=255)
  cGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAG‑CGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTC‑TTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGTTGATGTGGTACTGCAAAGCGCGCGCAGC‑GTGGCGGTCGGGGGGATGCTCg                                                                                                                                                                                                                                       >  1:982328/1‑239 (MQ=255)
   ggggTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAG‑CGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTC‑TTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGC‑GTGGCGGTCGGGGTGATGCCCgg                                                                                                                                                                                                                                      >  1:675823/1‑239 (MQ=255)
   ggggTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAG‑CGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTC‑TTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGC‑GTGGCGGTCGGGGGGCTGCTCgg                                                                                                                                                                                                                                      >  1:85296/1‑239 (MQ=255)
   ggggTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAG‑CGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTC‑TTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGC‑GTGGCGGTCGGGGGGATGCCCgg                                                                                                                                                                                                                                      >  1:870820/1‑239 (MQ=255)
               cTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAG‑CGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTC‑TTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGACGTTAATCGCGTCGCACCTATTACTACCGGTGATTTGGTACTGCAAAGCGCGCGCAGC‑GTGGCGGTCGGGGGGATGCGCGGTATCGCACCGgc                                                                                                                                                                                                                          >  1:1062246/1‑239 (MQ=255)
                 ggTCACAGTATTGTCAGTGATGAACGGCTTTGAG‑CGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTC‑TTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGC‑GTGGCGGTCGGGGGGATGCTCGGTATCGACCCGgcgc                                                                                                                                                                                                                        >  1:807962/1‑239 (MQ=255)
                    caccgttttttcggggatgaACGGGTTTGAG‑CGCGAGGTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTC‑TTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAAGCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGC‑GTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCaaa                                                                                                                                                                                                                     <  2:982328/224‑1 (MQ=255)
                     acaGTATTGTCAGTGAGGAACGGCTTTGAG‑CGCTAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTC‑TTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGC‑GTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCaaa                                                                                                                                                                                                                     <  1:855997/238‑1 (MQ=255)
                       aGTATTGTCAGTGATGAACGGCTTTGAG‑CGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTC‑TTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGGGATGTGGGACTGCAAAACGGGCGCAGC‑GTGCGGGTCGGGGGGGTGCGCGGGTTCGGCCCCGCCCAAAAAg                                                                                                                                                                                                                  >  2:830006/1‑239 (MQ=255)
                        tttttggtgtgggtgaACGGCTTTGAG‑CGGGGGTTGCAAAACAACATCCTTGGGCGGATGCCACAGGCAATTCTCTC‑TTCTGAGCATGGCTCTCTTAACCCGCAGCAACCCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGC‑GTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGa                                                                                                                                                                                                                 <  2:870820/226‑1 (MQ=255)
                        ttATTGTCAGTGATGAACGGCTTTGAG‑CGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTC‑TTCGGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGC‑GTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGa                                                                                                                                                                                                                 <  1:302/238‑1 (MQ=255)
                          aTTGTCAGTGATGAACGGCTTTGAG‑CGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTC‑TTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGC‑GTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGa                                                                                                                                                                                                                 <  1:291006/237‑1 (MQ=255)
                            tGTCAGTGATGAACGGCTTTGAG‑CGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTC‑TTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAACCGCGCGCAGC‑GTGGCGGCCGGGGTGATGTTCCGTATCCGCCCGGCGCAAAAAGATccc                                                                                                                                                                                                             >  1:55872/1‑238 (MQ=255)
                            tGTCAGTGATGAACGGCTTTGAG‑CGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTC‑TTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGCCGCACCTATTACTCCCGGTGAGTTGGCACTGCAAAACGCGCCCACCGGGGGCGTTCGGGGGTCTGCCCGGGTGAGACCCGGCGCCAAAAGaac                                                                                                                                                                                                               >  2:765987/1‑236 (MQ=255)
                            tGTCAGTGATGAACGGCTTTGAG‑CGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTC‑TTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGGCAAACTGGACGGCGTTAGTCGCGTCGCACCTATTACAACCGGTGCTGTGGTACTCCAAAACACGCGCGGC‑GGGGCGGCCAGGGGGGTGTGCGGAGACCACCCGGCGGGAAAAGAACCa                                                                                                                                                                                                             >  2:473144/1‑239 (MQ=255)
                              tCAGTGATGAACGGCTTTGAG‑CGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTC‑TTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGC‑GTGGCGGTCGGGGGGATGGTCGGTATCGACCCGGCGCAAAAAGATCCCCt                                                                                                                                                                                                           >  1:1030843/1‑239 (MQ=255)
                                 gTGATGAACGGCTTTGAG‑CGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTC‑TTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGAGCGCCCAGC‑GTGGCGGTCGGGGGGATGCTCGGTATCGACCCCGCGCACAAAAAACCACTTa                                                                                                                                                                                                         >  1:1006232/1‑238 (MQ=255)
                                 gTGATGAACGGCTTTGAG‑CGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTC‑TTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGAGTCGCACCTATTACTACCGGTGATTTGGTACTGCAAAGCGCGCGCAGC‑GTGGCGGGCGGGGGGAGGCTCGGCGTTGACCCGGCTCAAAAAAATCCACTTac                                                                                                                                                                                                        >  1:551651/1‑239 (MQ=255)
                                  tgttctccctgctttagaccgtggCTTCAAAACACCTTCCTTGGCCTCGTGCCACAGGCAATTC‑CTCTTTCTGGGCATGGTCCGCTTACCCCGCAGCAACTCCCAAAAACGGCAGTCAAACTGGACGGCCTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGC‑GTGGCGGTCGGGGTGATGCTCGGTAGCGACCCGGCGCAAAAAGATCCACTTac                                                                                                                                                                                                        <  2:1105358/216‑1 (MQ=255)
                                   gttgaACGGCTTTGGGCCGCG‑GCTGCAAAAAAACATCCTTGGCCGGATGCCCAAGGCAATTCTCTC‑TTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGC‑GTGGCGGTCGGGGTGATGCCCGGTATCGACCCGGCGCAAAAAGATCCACTTacac                                                                                                                                                                                                      <  2:673070/237‑1 (MQ=255)
                                   gatgaACGGCTTTGTG‑CGTGCGTTTGAAAACTAATCCCTTGGCCTGAGGCCACAGGCAAGTCTCTC‑TTGTGAGCCTGGCTCTCTTAACCCGCAGCAACTCCCAGAGACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCGCCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGC‑GTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTacac                                                                                                                                                                                                      <  2:274027/239‑1 (MQ=255)
                                   gatgaACGGCTTTGAG‑CGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTC‑TTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGC‑GTGGCGGTCGGGGGGATGCCCGGGATCGACCCGGCCCAAAAAGAACCCCTTacc                                                                                                                                                                                                       >  1:673070/1‑237 (MQ=255)
                                    atgaAGGGGTTTGAG‑CGCGGGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATGCTCTC‑TTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGC‑GTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTacac                                                                                                                                                                                                      <  1:999128/238‑1 (MQ=255)
                                             tttGAC‑CGCGGGCTGCAAATCAACATCCTTGGCCTGATGCCACCGGCAATTCTCTC‑TTCTGGGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATTGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGC‑GTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGt                                                                                                                                                                                            <  2:295846/239‑1 (MQ=255)
                                              ttGAG‑CCCGGGCTGAAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTC‑TTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGC‑GTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGt                                                                                                                                                                                            <  1:934409/238‑1 (MQ=255)
                                                              aaaaCAACATCCTTGGCCGGATGCCACAGGCAATTCTCTC‑TTCTGAGCATGGCTTTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGC‑GTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGaaacaaac                                                                                                                                                                             <  1:698353/238‑1 (MQ=255)
                                                                        ccTTTGCCTGACGCCACAGGCAATTTCTTC‑TTCTGAGCATGGCTCCTCTAACCCGCAGCAACCCCCAGAACCGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGC‑GTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGc                                                                                                                                                                  <  2:881863/239‑1 (MQ=255)
                                                                        ccTTGCCCTGAGGCCCCAGGCAATTCTCCC‑GTCGTAGCATGGCTGTCTTAACCCGCAGCAACCCCCAGAAACGGCAGTCAAACTGGACGGGGTTAATCGCGTCGCCCCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGC‑GTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGc                                                                                                                                                                  <  2:196129/239‑1 (MQ=255)
                                                                          ttggccgcattcaccaGGCAATTCTCCC‑TTCTGAGCATGGCCCTCTTAACCCGCAGCAACTCACAGAAACGGCAGTCAAACTGGGCGGCGTTAATCGGCGCGGACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGC‑GTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCg                                                                                                                                                                <  2:1018612/225‑1 (MQ=255)
                                                                          ttGGCCTGATGCCACAGGCAATTCTCTC‑TTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGC‑GTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCg                                                                                                                                                                <  1:765987/239‑1 (MQ=255)
                                                                             gCCTGCTGCCACCGGCAATTCTCTC‑TTCTGAGCAGGGCTCCCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGC‑GTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCgggg                                                                                                                                                             <  2:249251/239‑1 (MQ=255)
                                                                              ccTGATGCCACAGGCCACTCTCTT‑GTCTGGGCTGGGCTACTTTACCCCGCGGCAGCTCCCAGAGACGGGAGGCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACGGCCGGTGATGTGGTACTGCAGAGCGCGCGCAGC‑GTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCgggg                                                                                                                                                             <  2:82837/238‑1 (MQ=255)
                                                                                  aTGCCACAGGCAATTCTCTC‑TTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGC‑GTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCCAAAAGATCCCCCTACACCGTATCTGGTCAATGTGAAACAAAATGACCCCGAGCCGGGGaaaac                                                                                                                                                        >  1:208295/1‑237 (MQ=255)
                                                                                  aTGCCACAGGCAATTCTCTC‑TTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGC‑GTGGCGGTCGGGGGGATGCTCGGGATCGGCCCGGCGCCAAAAGATCCACTTACACCCGATCTGGGCAATGTGAAACAAACTGACCTCGAGCCGGGGAAata                                                                                                                                                        >  1:499348/1‑239 (MQ=255)
                                                                                          ggCAATTCTCTC‑TTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGC‑GTGGCGGTCGGGGTGATGCt                                                                                                                                                                                                                                         <  1:766603/150‑1 (MQ=255)
                                                                                              aTTCTCTC‑TTCTGAGCATGGCTCTCTTAACCCGCAGCAACCCCCAGAAACGGCAGGCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGC‑GTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCTACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCt                                                                                                                                            <  1:991729/239‑1 (MQ=255)
                                                                                                            aGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGC‑GTGGCGGCCGGGGTGATGGCCGGGATCTACCCGGCCCAAAAAAGACCCCTTAAACCGAAACTGGGCAACTGGGAACAAACCGAACACAAACCGGGGAAAAAAAAAGTCACACTCgcggaaaaaaag                                                                                                                               >  2:493496/1‑228 (MQ=255)
                                                                                                                       ctgaaCCCGCAGGAACTCCCAAGAACTGCAGTCAAACCGGACGGCGTTAAGCGCGTCGCTCCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGC‑GTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACA‑GCt                                                                                                                   <  1:143244/236‑1 (MQ=255)
                                                                                                                                cagcaACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGC‑GTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCCCA‑GCTAGGCGTTaa                                                                                                          >  1:681073/1‑239 (MQ=255)
                                                                                                                                cagcaACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGC‑GTGGCGGTCGGGGGGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAAGTTGAAACAAACTGACCTCGAGCCGGGGAAATATAAAGTCATCCTCGCGGAACAACTTGCCTCACA‑GCCAGGCGGTaa                                                                                                          >  1:412357/1‑239 (MQ=255)
                                                                                                                                     acccccGGAAACGGCAGTTAAACTGGACGGGCTTAAGCCGGGCGCACGTATTACTACCGGTGTTGTGGTACTGCAAAGCGCGCGCAGC‑GTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACCGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACA‑GCTAGGCGTTAATcgcg                                                                                                     <  2:397431/236‑1 (MQ=255)
                                                                                                                                     acccccAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGC‑GTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACA‑GCTAGGCGTTAATcgcg                                                                                                     <  1:754616/236‑1 (MQ=255)
                                                                                                                                     aCTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGC‑GTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACA‑GCTAGGCGTTAATcgcg                                                                                                     <  1:493496/239‑1 (MQ=255)
                                                                                                                                                   cAGTCAAACTGGAAGGCGTTTATCGCGTCGGACCTACTACTACCGGTGATGTGGCACTGCAAAGCGCGCGCAGC‑GTGGCGGTCGGGGTGATGCTCGGTATCGACGCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCGCGAGCCGGGGAAATATAATGTCATCCTCGGTGAACAACTTGCCTCACA‑GCTAGGCGTTAATCGCGGTGATCAAATCcg                                                                                        <  1:868100/238‑1 (MQ=255)
                                                                                                                                                     atccatcgggAGGGGGTTAATCGCGTCGCCCCTTTTACGACCGGGGATGTGGTACTGCAAAGCGCGCGCAGC‑GTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACA‑GCTAGGCGTTAATCGCGGTGATCAAATCCGCGt                                                                                     <  2:1062246/231‑1 (MQ=255)
                                                                                                                                                       aaaaCTGGACGGCGTTAATCGCGTGGCCCCTATTACTACCGGTGAGGTGGTACTGCAAAGCGCGCGCAGC‑GTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACA‑GCTAGGCGTTAATCGCGGTGATCAAATCCGCGTG‑a                                                                                  <  2:85296/238‑1 (MQ=255)
                                                                                                                                                               gcGGGGTTAATCGCGTCGCACCTATTTCTACCGGTGATGTGGTACGGCAAAGCGCGCGCAGC‑GTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACA‑GCTAGGCGTTAATCGCGGTGATCAAATCCGCGTG‑ATGGTAcc                                                                           <  1:196772/237‑1 (MQ=255)
                                                                                                                                                                             gTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGC‑GTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACt                                                                                                                                                                                                           <  2:956748/98‑1 (MQ=255)
                                                                                                                                                                             gTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGC‑GTGGCGGTCGGGGTGATGCCCGGTATCGACCCGGCGCAAAAAGATCCACt                                                                                                                                                                                                           >  1:956748/1‑98 (MQ=255)
                                                                                                                                                                               ctgtccTTTTCCTACCTGTGATGGGCTCCTCCAAAGCGCGCGCGGT‑GTGGCGGTGGGGGTGATGCCGGGTATCGACCCGGCGCAAAAAGGTCCCCTTACACCGTATCGGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACA‑GCTAGGCGTTAATCGCGGTGATCAAATCCGCGTG‑ATGGTACCATCTGCCAGCCAGTTCa                                                          <  2:499348/235‑1 (MQ=255)
                                                                                                                                                                                gCACCTATTACTGCCGGTGATGGGGTCCTGCAAAGGGGTCGCAGC‑GTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACA‑GCTAGGCGTTAATCGCGGTGATCAAATCCGCGTG‑ATGGTACCATCTGCCAGCCAGTTCa                                                          <  1:962448/238‑1 (MQ=255)
                                                                                                                                                                                  cccTATTACGACCGGTGATGTGGTACGGCAAAGCGCGCGCAGC‑GTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACA‑GCTAGGCGTTAATCGCGGTGATCAAATCcgcg                                                                                      <  1:42803/208‑1 (MQ=255)
                                                                                                                                                                                      aTTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGC‑GGTGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACA‑GCTAGGCGTTAATCGCGGTGATCAAATCCGCGTG‑ATGGTAcc                                                                           <  1:621344/215‑1 (MQ=255)
                                                                                                                                                                                            ccgggtgggggggtcatGCAAACGGCGCGGGGC‑GTGGCTGTCGGGGGGATGCTGGAGATCGACCCGGCGCAAAAAGGTCCCCTTCGACCTTAGCTGGTCAATGTGAAACAAACGGCCCTCGAGCCGGGGAAATATAATGTCATCCTGGGCGAACAACTTGCCTCACA‑GCTGGGCGTGAACCGCGGTGATCAAATCCGCGTG‑ATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCg                                             <  2:869910/223‑1 (MQ=255)
                                                                                                                                                                                              cGGTGATGTGGTACTGCAAAGCGCGCGCAGC‑GTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACA‑GCTAGGCGTTAATCGCGGTGATCAAATCCGCGTG‑ATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTa                                           >  1:771278/1‑239 (MQ=255)
                                                                                                                                                                                              cGGTGATGTGGTACTGCAAAGCGCGCGCAGC‑GTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACA‑GCTAGGCGTTAATCGCGGTGATCAAATCCCCGTG‑ATGGTACCATCTGCCAGCCCGTTCACGCCGATGGGGCGTa                                           >  1:681485/1‑239 (MQ=255)
                                                                                                                                                                                                gTGATGTGGTACTGCAAAGCGCGCGCAGC‑GTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGaaca                                                                                                                                   >  2:143245/1‑151 (MQ=255)
                                                                                                                                                                                                gTGATGTGGTACTGCAAAGCGCGCGCAGC‑GTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGaaca                                                                                                                                   <  1:143245/151‑1 (MQ=255)
                                                                                                                                                                                                gTGATGTGGTACTGCAAAGCGCGCGCAGC‑GTGGCGGTCGGGGGGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAACATGACCTCCAAACGGGGAAAAATAATGTCATCCTCTGCGAACAACTTTCCTCACA‑GCTAGGCGTTAATCGCGGGGATCAAAACCGCGCG‑CTGGGACACTCTgcccgccccgtctcttatacacaacgcaa                                          >  2:621344/1‑216 (MQ=255)
                                                                                                                                                                                                 tGATGTGGTACTGCAAAGCGCGCGCAGC‑GTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCGTCCTCGGCGAACAACTTGCCTCACA‑GCTAGGCGGTAATCGCGGTGATCAAATACGCGGG‑CTGGTACCTTTTGCCAGCCAGTTCACGCCCATTGGGCGGATTc                                        >  1:251619/1‑239 (MQ=255)
                                                                                                                                                                                                  gATGTGGTACTGCAAAGCGCGCGCAGC‑GTGGCGGTCGGGGTGATGCt                                                                                                                                                                                                                                         <  2:454393/47‑1 (MQ=255)
                                                                                                                                                                                                  gATGTGGTACTGCAAAGCGCGCGCAGC‑GTGGCGGTCGGGGTGATGCt                                                                                                                                                                                                                                         >  1:454393/1‑47 (MQ=255)
                                                                                                                                                                                                   gtggggTACCGCAAAGCGCGCGCCGC‑GTGGCGGTCGGGTTGATGCCCGGTAACGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACA‑GCTAGGCGTTAATCGCGGTGATCAAATCCGCGTG‑ATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTcc                                       <  1:473144/234‑1 (MQ=255)
                                                                                                                                                                                                          aCTGCAAAGCGCGCGCAGC‑GTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACA‑GCTAGGCGTTAATCGCGGGGATCAAATCCGCGTG‑ATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGGTGATTCCAAGCCAg                                >  1:565393/1‑238 (MQ=255)
                                                                                                                                                                                                              cAAAGCGCGCGCAGC‑GTGGCGGTCGGGGTGATTCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGGTAATGTGAAAAAAACTGACCTCGAGCCGGGGAAATATAATGTCCACCTCGTCGAACAACTTGCCTCAAC‑GCCAACAGTTAATCGCTGGGGTCACATACGCCGG‑ATGGTACACTCAGCCACACAGTTCACTCCCATAGGGCGGATccccaacaccgcccc                           >  2:454375/1‑227 (MQ=255)
                                                                                                                                                                                                              cAAAGCGCGCGCAGC‑GTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACA‑GCTAGGCGTTAATCGCGGTGATCAAATCCCCGTG‑ATGGTACCATCTGCCAGCCAGTTCACGCCGGTGGGGCGGATTCCAAGCCAgcg                              >  1:767121/1‑236 (MQ=255)
                                                                                                                                                                                                              cAAAGCGCGCGCAGC‑GTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACA‑GCTAGGCGTTAATCGCGGTGATCAAATCCCCGTG‑ATGGTACCATCTGCCAGCCAGTTCACGCCGAAGGTGCGGATTCCAAGCCCGCCCCt                           >  1:1070589/1‑239 (MQ=255)
                                                                                                                                                                                                              cAAAGCGCGCGCAGC‑GTGGCGGTCGGGGAGATGCTTGGCATCGGCCCCGCGCCAAAAGGACCACTTACCCCGGATCTGGTCAATGGGAAACAAACTGACTCCGAGACGGGGGAAAATAATTGCATCCTCGGGAAAAAACTTGAC‑CACACGCTAGGCGTTCAACGCGGGGGACAAAACCACGTG‑AGGGGAACACCTGCCAGCCGGATTACCCCGATGGGGGGGATTCCaaacgaaggccc                           >  2:884758/1‑229 (MQ=255)
                                                                                                                                                                                                                aaGCGCGCGCAGC‑GTGGCGGTCGGGGGGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACA‑GCTAGGCGTTAATCGCGGTGATCAAATCCGCGTG‑ATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGGATTCCAAGCCAACGCCTGt                         >  1:818493/1‑239 (MQ=255)
                                                                                                                                                                                                                   cgggcgcAGG‑GTGGGGGTCGGGGGGATTCTCGGTAGCGCCCCGGCGCAAAAATATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACA‑GCTAGGCGTTAATCGCGGTGATCAAATCCGCGTG‑ATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCa                      <  2:681073/236‑1 (MQ=255)
                                                                                                                                                                                                                   cgcgcgcAGC‑GTGGCGGTCGGGGTGATTCTCGGTATCGACCCGGCGCAAAAAGATACACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACA‑GCTAGGCGTTAATCGCGGTGATCAAATCCGCGTG‑CTGGTACCATCTGCCAGCCCGGTCACGCCGGTGGGGGGGATTCCacacacacgccctttca                      >  2:93398/1‑223 (MQ=255)
                                                                                                                                                                                                                   cgcgcgcAGC‑GTGGCGGTCAGGGTGATGCTCGGTATCGACCCGGCGCCAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGGGCCGGGGAAATATTATGTCATCCTCGGCGAAAAAATTTCCCCACA‑GCTAGGCGTTAATCGCGGGGATCAAATCCCCCTG‑ATGGGACCAACCGCCACCCATTTCACGCCGGTGGGGGGTATTCCAAGCCAACGCCGGGTCa                      >  2:17265/1‑239 (MQ=255)
                                                                                                                                                                                                                     cgcgcAGC‑GTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCCCTTACACCGTATCTGGTCAAAGTGAAACAAACTGACTTCGAGCCGGGGAAATTTAATGTAATCCTGGCCGAACAACTTGCCTCCAA‑GCTGAGCGTTAATCTGGGAGATCAAAACAG‑GTGCTTGGTACCACGACCCACCCAGTTTAACCCGATGGGGGGGTTTCCAATCCA‑CGCCCGtttcaaa                   >  2:804818/1‑234 (MQ=255)
                                                                                                                                                                                                                     cgcgcAGC‑GTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCCAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACA‑GCTAGGCGTTAATCTCGGTGACCAAATCCCCCGC‑TTGGTACCCACTCCCCGCCAGTTCACGCCCATAGGGCGGATTCCAAACCAGCGCCCCTTCaaa                    >  2:203381/1‑238 (MQ=255)
                                                                                                                                                                                                                      ccctcggtctGGCGGTCGGGGTTATTCTCGGGATCGCCCCGGCGAAAAAAGCTCCACTTCCCCGGTATCTGGTCAATGTGAAACAAACTGTCCGTGGGCCGGGGAAATATAATGTCAGCCTCGGCGAACAACTTGCCTCACA‑GCTAGGCGTTAATCGCGGTGATCAAATCCGCGTG‑ATGGTACCATCTGCCCGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAAt                    <  2:726759/230‑1 (MQ=255)
                                                                                                                                                                                                                                         ggTGATGCTAGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTACTTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACA‑GCTAGGCGTTAATCGCGGTGATCAAATCCGCGTG‑ATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTcgcc  <  1:804818/238‑1 (MQ=255)
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                       
CTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAG‑CGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTC‑TTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGC‑GTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACA‑GCTAGGCGTTAATCGCGGTGATCAAATCCGCGTG‑ATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCC  >  NC_000913/1175532‑1175999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A