Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F9 I0 R1 71 67.1 1514203 90.7% 1373382 220.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,196,131 G→A 20.3% A337T (GCC→ACC)  icd → isocitrate dehydrogenase, e14 prophage attachment site, tellurite reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,196,1310GA20.3% 86.9 / 21.5 51A337T (GCC→ACC) icdisocitrate dehydrogenase, e14 prophage attachment site, tellurite reductase
Reads supporting (aligned to +/- strand):  ref base G (25/15);  new base A (4/6);  total (30/21)
Fisher's exact test for biased strand distribution p-value = 2.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.68e-01

GGCCCGTGGCTGAAAGTTAAAAACCCGAACACTGGCAAAGAGATCGTCATTAAAGACGTGATTGCTGATGCATTCCTGCAACAGATCCTGCTGCGTCCGGCTGAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCG‑CCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACG  >  NC_000913/1195903‑1196353
                                                                                                                                                                                                                                     |                                                                                                                                                                                                                              
ggCCCGTGGCTGAAAGTTAAAAACCCGAACCCTGGCAAAGGGATCGGCATTAAAGCCGTGATTGCTGATGCATTCCTGCAACAGATCCTGCTGCGTCCGGCTGAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCG‑CCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAg                                                                                                                                                                                                                                <  1:325064/229‑1 (MQ=255)
ggCCCGTGGCTGAAAGTTAAAAACCCGAACACTGGCAAAGAGATCGTCATTAAAGACGTGATTGCTGATGCATTCCTGCAACAGATCCTGCTGCGTCCGGCTGAATATGATGGTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCG‑CCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAg                                                                                                                                                                                                                                <  1:478260/229‑1 (MQ=255)
  cccGTGGCTGAAAGTTAAAAACCCGAACACTGGCAAAGAGATCGTCATTAAAGACGTGATTGCTGATGCATTCCTGCAACAGATCCTGCTGCGTCCGGCTGAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCG‑CCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAg                                                                                                                                                                                                                                >  1:171300/1‑227 (MQ=255)
  cccGTGGCTGAAAGTTAAAAACCCGAACACTGGCAAAGAGATCGTCATTAAAGACGTGATTGCTGATGCATTCCTGCAACAGATCCTGCTGCGTCCGGCTGAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCG‑CCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAg                                                                                                                                                                                                                                <  2:171300/227‑1 (MQ=255)
   ccGTGGCTGAAAGTTAAAACCCCGAACACTGGCAAAGAGATCGTCATTAAAGACGTGATTGCTGATGCATTCCTGCAACAGATCCTGCTGCGTCCGGCTGAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCG‑CCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAAcc                                                                                                                                                                                                                              <  2:70402/228‑1 (MQ=255)
             aaGTTAAAACCCCGAACAGTGGCAAAGAGATCGTCATTAAAGACGTGATTGCTGATGCATTCCTGCAACAGATCCTGCTGCGTCCGGCTGAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCG‑CCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTa                                                                                                                                                                                                                    <  1:626002/228‑1 (MQ=255)
                   aaaaCCCGAACACTGGCAAAGAGATCGTCATTAAAGACGTGATTGCTGATGCATTCCTGCAACAGATCCTGCTGCGTCCGGCTGAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTGGCAGCGCAGGTTGGCGGCATCGGTATCC‑CCCCTGGTGCAAACATCGGTGACGAAGGCGCCCTGTTTGAAGCACCCCCCGGTACACCGcc                                                                                                                                                                                                             >  2:338850/1‑229 (MQ=255)
                                gggcaaagggtccttgaTTAAAGAGGTGATTGGCGTTGCATTCCTGGAACAGAGCCGGCTGCGTCCGGCTGAATATGATGTTATCGCCCGTATGCACCTGAACGGTGACTACATTTCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCG‑CCCCTTGTGCAAACATCGGGTACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGt                                                                                                                                                                                                <  2:109251/213‑1 (MQ=255)
                                  gCAAAGAGATCGTCATTAAAGACGTGATTGCTGATGCATTCCTGCAACAGATCCTGCTGCGTCCGGCTGAATCTGATGTTATCGCCTGTATGAACCTGAACGGTGACTCCATTTCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCG‑CCCCTGGTGCAAACATCGGGGACCAATAGGCCCTGTTTGAGGCCACCCACGGAACTgcgcagcaccaggccggcag                                                                                                                                                                                              >  1:595630/1‑213 (MQ=255)
                                          aTCGTCATTAAAGACGTGATTGCTGATGCATTCCTGCAACAGATCCTGCTGCGTCCGGCTGAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCG‑CCCCTGGTGCAAACATCGGGGACGAATGCGCCCGTTGTGAAGCCACCCACGGTACTGCGCCGCAACATGCCGGTC‑GGGCAAAg                                                                                                                                                                                      >  1:599777/1‑228 (MQ=255)
                                                  tAAAGACGTGATTGCTGATGCATTCCTGCAACAGATCCTGCTGCGTCCGGCTGAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCG‑CCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCCCGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCt                                                                                                                                                                              >  1:695097/1‑229 (MQ=255)
                                                      ggcGTGAATGCTGATGCATTCCTGCAACAGATCCTGCTGCGGCCGGCTGAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCG‑CCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAACCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGct                                                                                                                                                                          <  1:228646/227‑1 (MQ=255)
                                                         gTGATTGCTGATGCATTCCTGCAACAGATCCTGCTGCGTCCGGCTGAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCG‑CCCCTGGTGCAAACATCGGTTACGAATGCGCCCTGTTTGAAGCCACCCCCGGTACTTCCCCGAAATATGCCGGTCCGGCAAAAGGAAAACCAGGcccg                                                                                                                                                                        >  2:302450/1‑225 (MQ=255)
                                                         gTGATTGCTGATGCATTCCTGCAACAGATCCTGCTGCGTCCGGCTGAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCG‑CCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAACCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGGCAAAGTAAAACCTGGctct                                                                                                                                                                        >  1:673498/1‑228 (MQ=255)
                                                         gTGATTACTCCTGCATTCCTGCAACAGATCCTGCTGCCTCCGGCTGAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTGGCATCGCCGGTTGGCGGTATCGGTATTG‑ACCCTGGTGCAAACCACGGGGACGAATGCGGCCTGTTTGAAGCACCCCACGGTACTGGCCCGACAAATGCCGGTCAGGAACAAGAAAAACCGGGCGCta                                                                                                                                                                       >  2:234804/1‑229 (MQ=255)
                                                          tGATTGCTGATGCATTCCTGCAACAGATCCTGCTGCGTCCGGCTGAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCG‑CCCCTGGTGCCACCATCGGTGACGAATGCGCCCTGTTTGAAACCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGCCAACGTAAATCCTGCCTCta                                                                                                                                                                       >  1:306348/1‑228 (MQ=255)
                                                                  ggtGCTTTCCTGCAACAGATCCTGCCGCGTCCGGCTGAAGATGATGTTGTCGCCTATATGAACCTGAACGGTGACTTCATTTCTGACGACCTGGCAGCGCAGGTTGGCGGTATCGGTATCG‑CCCCTGGTGCAAATATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATtct                                                                                                                                                                <  2:467248/225‑1 (MQ=255)
                                                                  gaggCATTCCTGCAACAGATCCTGCTGCGTCCGGCTGAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCG‑CCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAACCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATtctct                                                                                                                                                              <  1:486997/226‑1 (MQ=255)
                                                                   aTGCATTCCTGCCAAAGATCCTGCTGCGTCCGGTTGAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCG‑CCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAACCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATtctctc                                                                                                                                                             <  2:306348/229‑1 (MQ=255)
                                                                      ccttCCTGCAACAGATCCTGCTGCGTCCGGCTGAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATTG‑CCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAACCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGc                                                                                                                                                          <  1:20147/227‑1 (MQ=255)
                                                                      cATTCCTGCAACAGTTCCTGCTGCGTCCGGCTGAATATGATGGTATCGCCTGCATGAACCTGAACGGTGGCTACATTTCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCG‑CCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGc                                                                                                                                                          <  1:325596/229‑1 (MQ=255)
                                                                                               tCCGGCTGAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCG‑CCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCCCCGCTGAGCTGATGCTGCGCCACATggtt                                                                                                                                 >  1:491627/1‑227 (MQ=255)
                                                                                               tCCGGCTGAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCG‑CACCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAACCCACCCACGGTACTGCGCCGAAATATGCCGGGCCGGCAAAAGTACTACCTGGCTTTCTTCTTCTCACCGCTGAAGTGATGGTGGGCCAAATGGGt                                                                                                                                 >  2:693355/1‑229 (MQ=255)
                                                                                                  ggCTGAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCG‑CCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTCCTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTTTCCGCTTAGATGGTGCTGCGCCACCTGGGTTgt                                                                                                                              >  2:40540/1‑228 (MQ=255)
                                                                                                  ggCTGAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCG‑CCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTg                                                                                                                               >  1:173239/1‑228 (MQ=255)
                                                                                                              tgTTATCGCCTGTATGAACCTGAACGGAGACTACATTTCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCG‑CCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGGTTGAAGCCACCCACGGTACTTCGCCGAAATATGCCGGTCAGGAGAACGTAAATCCTGGCTCAATTATTCTCTCCGCGGGGATGATGCTGCGACACAGGGGTTGGACCGAAGCgccc                                                                                                                  >  1:747388/1‑226 (MQ=255)
                                                                                                                  aTCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCG‑CCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACt                                                                                                              >  1:477542/1‑229 (MQ=255)
                                                                                                                      cttttttGAACCTGACAGGTTACTACTTTTCGTGCGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCGCCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAACCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTaa                                                                                                           <  2:327795/223‑1 (MQ=255)
                                                                                                                          tATGAACCTGAACGGTGACTACATTTCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCG‑CCCCTGGTGCAAACATCGGTTACGAATGCGCCCTGTTTGAAACCACCCACGGCACTGCGCCGAAATAAGCCTGTCCGGACAAAGTAAATCCTGGCTCTATTATTCACTCCGCTTCGATGAGTCTGCGCCACCTGGGTTGGACCggaccagccgcacttattttt                                                                                                      >  2:351727/1‑209 (MQ=255)
                                                                                                                          tATGAACCTGAACGGTGACTACATTTCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCG‑CCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAAAATGCCGGTCAGGACAACGAAAATCCTGGCCCTATTATTCTCTCCGCTGGGATGATGTTCAGCCAAATGGGGTGGACCGAACGGGCTGACTTAATTGtt                                                                                                      >  2:250968/1‑229 (MQ=255)
                                                                                                                          tATGAACCTGAACGGTGACTACATTTCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCG‑CCCCTGGTGCAAACATCGGTGACGAATGCGCCATGTTTGAAGCCCCCCGCGGGACTGCGCCGAATTATGCCGGTCAGGACAACGTAAATCCTGGCTCTGTTCTTCTCTCCGCTGAGATGATGCTGCACCACATGGGTTGGGCGCAATAGCCTGCCTTAATtttt                                                                                                      >  2:606439/1‑229 (MQ=255)
                                                                                                                          tATGAACCTGAACGGTGACTACATTTCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGGATCG‑CCCCTGGTGCAAACATCGGGGAAGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATTATGCGCCACATGGGTTGTACCGAAGCGGCTGACTTAATTGtt                                                                                                      >  2:673414/1‑229 (MQ=255)
                                                                                                                           aTGAACCTGAACGGTGACTACATTTCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCG‑CCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTa                                                                                                     <  2:477542/229‑1 (MQ=255)
                                                                                                                                ccTGAACGGTGACTACATTTCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCG‑CCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCCATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGCAGCGGCTGCCTTAATTGTTTAAGGt                                                                                                >  1:698624/1‑229 (MQ=255)
                                                                                                                                    aaCGGTGACTACATTTCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCG‑CCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCAGCTGGATTACTTGTTACAggactgg                                                                                            >  1:299598/1‑224 (MQ=255)
                                                                                                                                    aaCGGTGACTACATTTCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCG‑CCCCTGGGGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGGGATGATGCTGCGCCACATGGGTTGGACGGAAGCGGCTGACTTAATTGTTAAAggaaggg                                                                                            >  1:250237/1‑224 (MQ=255)
                                                                                                                                               cATTTCTGACGCCCTGGCAGCTCAGGTTGGCGGTATCGGTATCG‑CCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATc                                                                                 <  1:302450/229‑1 (MQ=255)
                                                                                                                                                   tCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCG‑CCCCTGGTGCAAACATCGGTGACGAAGGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCCCTATTATTCGCTCCCCTGAAATGATGCTGCGCCACATGGGTTGGACCGAGGCGGCTGCCTTAATAGTTAAGGGGATGGAGGGCGCAATAAAcg                                                                             >  1:344937/1‑229 (MQ=255)
                                                                                                                                                      gACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCG‑CCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAACCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGa                                                                          <  1:184958/229‑1 (MQ=255)
                                                                                                                                                       gcGCCCTGGCCGCGCAGGTTGGCGGTTTCGGTATCG‑CCCCTGGTGCAAACATCGGGGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACCGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCGCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGa                                                                          <  2:698623/227‑1 (MQ=255)
                                                                                                                                                       aCGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCG‑CCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGGATGGAAGGCGCAATCAAcgcg                                                                           >  1:509026/1‑227 (MQ=255)
                                                                                                                                                                  gcgcGGGTTGGGGGTTTCGGTATCG‑CCCCTGGTGCAAACCTCGGTGACGAATGCGCCCTGGTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCt                                                              <  2:305732/229‑1 (MQ=255)
                                                                                                                                                                   cgcAGGTTGGCGGTATCGGTATCG‑CCCCTGGTGCAAACATCGGTGACGAATGCGGCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATCCTGCGCCACAAGGGTTGGACCGAAGCGGCTGACCGAATTGTTAAAGGGATGAAAGGGGCAACCAAAGGCAAAACCTTAAccat                                                             >  2:352040/1‑227 (MQ=255)
                                                                                                                                                                    gcAGTTTGGCTGTATCGGTATCG‑CCCCTGGTGCAAACAACGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCGCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTAt                                                            <  2:684346/229‑1 (MQ=255)
                                                                                                                                                                              ggtaacggtatcG‑TCCCTGGTGCAAACATCGGTGACGAATGCGACCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTACCCTATGACTTCGa                                                    <  1:606440/227‑1 (MQ=255)
                                                                                                                                                                                         tccccccTGGTGCAAACAGCGGTGAAGAATGCGCCCTGTTTGAAGCCACCCACGGTAATGCCCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTgatgga                                        <  2:173239/226‑1 (MQ=255)
                                                                                                                                                                                         cg‑CCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTgatgga                                        <  1:673415/228‑1 (MQ=255)
                                                                                                                                                                                          g‑CCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTAGAGTGTCTgctgg                                         >  1:220528/1‑226 (MQ=255)
                                                                                                                                                                                            ccccTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGCCTTAATTGTTAAAGGTATGGAAGGGGCAACCACCCCGGAAACCGTAACCCATAACTTagaggtgcggagggtggg                                     >  2:351459/1‑215 (MQ=255)
                                                                                                                                                                                                   gtcAAACCTCGGTGACGAATGCGCCCTGGTGTAAGCCACCCCCGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTaaa                              <  1:338850/227‑1 (MQ=255)
                                                                                                                                                                                                       aaCATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATCCTGCGCAACATGTGTTCGACCGAAGCGTCTGCCTTAATTGTTAAAGGTATGAAAGGCGCAATCAAAGCGAAAACCATAAACAATGACTTAGCGAGGCTgatggcaacggccaaaaggc                          >  2:423007/1‑214 (MQ=255)
                                                                                                                                                                                                            cGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCCATCAACGCGAAAACCGTAAACTATGACTTCGAGTGTCGGATGGTGGGCGCTAAAACGCGGaaa                     >  2:636194/1‑229 (MQ=255)
                                                                                                                                                                                                             ggTGACGAATGCGCCCTGTTTGAAACCACCCGCGGTACTGCGCCGAAATATGCCGGTCCGGACAACGTAACACCTGGCTCTATTCTTCTCTCCGCTGAGCTGATGCTGCGCCACATGGGTTGGACCGCACCGGCTGACTTAGTTGTTAAAGTGATGGCAGGGGCAATCAACGCGAAAACCACAACCTATGACTCCGAGCGTGTTAGGGATGGTGCTAAACAGCTGaaaa                    >  2:497512/1‑228 (MQ=255)
                                                                                                                                                                                                                    aaTGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGCCCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGACATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAAACCCTGAAATGTTCaga             >  1:401861/1‑229 (MQ=255)
                                                                                                                                                                                                                               tttGAAGCCACCCCCGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGAcg  <  2:446582/229‑1 (MQ=255)
                                                                                                                                                                                                                                     |                                                                                                                                                                                                                              
GGCCCGTGGCTGAAAGTTAAAAACCCGAACACTGGCAAAGAGATCGTCATTAAAGACGTGATTGCTGATGCATTCCTGCAACAGATCCTGCTGCGTCCGGCTGAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCG‑CCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACG  >  NC_000913/1195903‑1196353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A