Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F17 I0 R1	160	98.0	2214160	89.0%	1970602	222.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,511,862	T→G	18.1%	V70G (GTG→GGG)	ydcS →	putative ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,511,862	0	T	G	18.1%	150.4 / 3.2	80	V70G (GTG→GGG)	ydcS	putative ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand): ref base T (48/14); new base G (8/9); total (56/24)
Fisher's exact test for biased strand distribution p-value = 3.17e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGGAGTAAGACCTTATGAGCAAGACATTTGCCCGCAGCAGCCTGTGTGCGCTCAGCATGACAATAATGACCGCTCACGCCGCCGAACCGCCTACCAATTTAGATAAACCGGAAGGGCGACTGGATATTATCGCCTGGCCGGGATACATCGAACGCGGACAAACTGATAAACAATACGACTGGGTAACG‑CAGTTCGAAAAAGAGA‑CA‑GGCT‑GCGCGGTGAA‑TGTGAAAA‑CCGC‑CGCGACTTCCGATGAAATGGTCAGTCTGATGACCAAAGGGGGTTACGATCTGGTTACGGCATCC‑GGCGATGCCTCGCTGCGTTTGATTATGGGTAAACGCGTGCAGCCGATTAATACCGCATTGATT‑CCCAACTGGAAAACGCTCGATCCGCGCGTGGTTAAAGGCGACTGGTTTAATGTTGGCGGCAAAGTTTACGGCACACCTTACCAATGGGGG  >  NC_000913/1511639‑1512088

cAGGAGTAAGACCTTATGAGCAAGACATTTGCCCGCAGCAGCCTGTGTGCGCTCAGCATGACAATAATGACCGCTCACGCCGCCGAACCGCCTACCAATTTAGATAAACCGGAAGGGCGACTGGATATTATCGCCTGGCCGGGATACATCGAACGCGGACAAACTGATAAAAAATACGACCGGGTAACG‑CAGTTCGAAAAAGAGA‑AG‑GGCTAGCG‑GGTGAA‑TGGGAAAA‑ccgc‑caggaa                                                                                                                                                                                                                       >  2:987413/1‑234 (MQ=255)

cAGGAGTAAGACCTTATGAGCAAGACATTTGCCCGCAGCAGCCTGTGTGCGCTCAGCATGACAATAATGACCGCTCACGCCGCCGAACCGCCTACCAATTTAGATAAACCGGAAGGGCGACTGGATATTATCGCCTGGCCCGGATACATCGAACCCGGACAAACAGATAAACAATACGACTGGGTAAAC‑CCGTTCGAAAAAAAGA‑CA‑GGCA‑GCGCGAGGAA‑TTGGAAAA‑cccacccgcg                                                                                                                                                                                                                        >  2:194717/1‑231 (MQ=255)

   gAGTAAGACCTTATGAGCAAGACATTTGCCCGCAGCAGCCTGTGTGCGCTCAGCATGACAATAATGACCGCTCACGCCGCCGAACCGCCTCCCACTTTAGATAAACCGGAAGGGCGACTGGATATTATCGCCTGGCCGGGATACATCGAACGCGGACAAACTGCTAAACAATACGAATGGGTAACG‑CGGTTCTAAAAAAAGA‑CA‑GGGC‑GCGCGGGGAG‑TTGGAAAA‑CCCC‑cgcgcatc                                                                                                                                                                                                                     >  2:558610/1‑234 (MQ=255)

      tAAGACCTTATGAGCAAGACATTTGCCCGCAGCAGCCTGTGTGCGCTCAGCATGACAATAATGACCGCTCACGCCGCCGAACCGCCTACCAATTTAGATAAACCGGAAGGGCGACTGGATATTATCGCCTGGCCGGGATACATCGAACGCGGACAAACTGATAAACAATACGACTGGGTAACG‑CAGTTCGAAAAAGAGA‑CA‑GGCT‑GCGCGGTGAA‑TGTGAAAA‑CCGC‑CGCGACTTCCGa                                                                                                                                                                                                                 >  1:624230/1‑239 (MQ=255)

          actttAGGAGCAAAACATTGCCCCGCAGCAGCCTGTGTGCGCTCAGCATAACAATAAGGACCGCCACCGCCGCCGACCCGCCTCCCAATTAAGATAAACCGGAAGGGCGACTGGATATTATCGCCGGGCCGGGATACATCGAACGCGGAAAAACTGAAAAACAATCCGACTGGGTAACG‑CAGTTCGAAAAAGAGA‑CA‑GGCT‑GCGCGGTGAA‑TGTGAAAA‑CCGC‑CGCGACTTCCGATGaa                                                                                                                                                                                                             <  1:878955/236‑1 (MQ=255)

             ttATGAGCAAGACATTTGCCCGCAGCAGCCTGTGTGCGCTCAGCATGACAATAATGACCGCTCACGCCGCCGAACCGCCTACCAATTTAGATAAACCGGAAGGGCGACTGGATATTATCGCCTGGCCGGGATACATCGAACGCGGACAAACTGATAAAAAATACGACTGGGTAACG‑CAGTTCGAAAAAGAGA‑CA‑GGCT‑GCGCGGTGAA‑TGTGAAAA‑CCGC‑CGCGACTTCAGAGGaaagg                                                                                                                                                                                                          >  2:698974/1‑237 (MQ=255)

              tattagcaacggaTTTGCCCGCAGCAGCCTGTGTGCGCTCAGCATGACAATAATGCCCGCTCACGCCGCCGAACCGCCTACCAATTTAGATAAACCGGAAGGGCAACGGGCAATTATCGCCTGGCCGGGCTACATCGAACACGGCCAAGCTGTGAAACAATACGCCTGGGACACG‑CAGAT‑GGAAAAGAGACCC‑GGCC‑ACACGGTGAA‑TTGAAAAA‑CCCC‑CGCACCTTcccaaaaaagga                                                                                                                                                                                                         >  2:1040107/13‑229 (MQ=255)

              tATGAGCAAGACATTTGCCCGCAGCAGCCTGTGTGCGCTCAGCATGACAATAATGACCGCTCACGCCGCCGAACCGCCTACCAATTTAGATAAACCGGAAGGGCGACTGGATATTATCGCCTGGCCGGGATACATCGAACGCGGCAAAACTGATAACCCATACGCCCGGGCAAAG‑CAGTTCGAAAAAACAA‑CA‑GGCC‑CCCCGGGGAA‑AGGGGAAA‑cccc‑ccccgaacccgaagaaacgg                                                                                                                                                                                                         >  2:959842/1‑220 (MQ=255)

              tATGAGCAAGACATTTGCCCGCAGCAGCCTGTGTGCGCTCAGCATGACAATAATGACCGCTCACGCCGCCGAACCGCCTACCAATTTAGATAAACCGGAAGGGCGACTGGATATTATCGCCTGGCCGGGATACATCGAACGCGGACAAACTGATAAACAATACGACTGGGTAACG‑CAGTTCGCAAAAGAGA‑CA‑GGCT‑TCCCGGGGAA‑TGGGAAAA‑ACGC‑CCCGACCTCCCAAGAAAAgg                                                                                                                                                                                                         >  2:994882/1‑239 (MQ=255)

              tATGAGCAAGACATTTGCCCGCAGCAGCCTGTGTGCGCTCAGCATGACAATAATGACCGCTCACGCCGCCGAACCGCCTACCAATTTAGATAAACCGGAAGGGCGACTGGATATTATCGCCTGGCCGGGATACATCGAACGCGGACAAACTGATAAACAAAACGACTGGGTACCG‑CAGTTCGAAAAAGAGA‑CC‑GGCT‑GCGCGGAGAA‑TGTGAAAA‑CAGA‑CGCGACCTCCAATGAAATgg                                                                                                                                                                                                         >  2:435008/1‑239 (MQ=255)

                    cAAGACATTTGCCCGCAGCAGCCTGTGTGCGCTCAGCATGACAATAATGACCGCTCACGCCGCCGAACCGCCTACCAATTTAGATAAACCGGAAGGGCGACTGGATATTATCGCCTGGCCGGGATACATCGAACGCGGACAAACTGATAAACAATACGACTGGGTAACG‑CAGTTCGAAAAAAAGA‑CA‑GGCT‑GCGCGGTGAA‑TGTGAAAA‑CCGC‑CGCGACTTCCGCTGAAATGGTCAg                                                                                                                                                                                                     >  1:966116/1‑237 (MQ=255)

                       gACATTTGCCCGCAGCAGCCTGTGTGCGCTCAGCATGACAATAATGACCGCTCACGCCGCCGAACCGCCTACCAATTTAGATAAACCGGAAGGGCGACTGGATATTATCGCCTGGCCGGGATACATCGAACGCGGACAAACTGATAAACAATACGACTGGGTAACG‑CAGTTCGAAAAAGAGA‑CA‑GGCT‑GCGCGGTGAA‑TGTGCAAA‑CCGC‑CGCGACTTCCGATGAAATGGTCAGTAtga                                                                                                                                                                                                >  1:518244/1‑239 (MQ=255)

                            ttGCCCGCAGCAGCCTGTGTGCGCTCAGCATGACAATAATGACCGCTCACGCCGCCGAACCGCCTACCAATTTAGATAAACCGGAAGGCCAACTGGATATTATCGCCTGCCCGGGATACATCCAACCCGGCCAAAATGTTAACCAAT‑CGAATGGGGAAAGCCAATTCGAAAAGGAGA‑CA‑GGCG‑CCGCGGCTAA‑TTGGAAAA‑CACC‑CGCGACATCCGAGAAAAGGGtaacccttgtgaca                                                                                                                                                                                           >  2:403155/1‑226 (MQ=255)

                                               tgCGCTCAGCATGACAATAATGACCGCTCACGCCGCCGAACCGCCTACCAATTTAGATAAACCGGAAGGGCGACTGGATATTATCGCCTGGCCGGGATACATCGAACGCGGACAAACTGATAAACAATACGACTGGGTAACG‑CAGTTCGAAAAAGAGA‑CA‑GGCT‑GCGCGGTGAA‑TGTGAAAA‑CCGC‑CGCGACTTCCGATGAAATGGTCAGTCGGATGACCAAAGGGGGTTAAGATCTgt                                                                                                                                                                        >  1:473333/1‑238 (MQ=255)

                                                       gCATGACAATAATGACCGCTCACGCCGCCGAACCGCCTACCAATTTAGATAAACCGGAAGGGCGACTGGATATTATCGCCTGGCCGGGATACATCGAACGCGGACAAACTGATAAACAATACGCATGGGTAACG‑CAGTTCGAAAAAAAGA‑CA‑GGCT‑TCGCGTTGAA‑GGTGAAAA‑CCGC‑CGCGACTTCCGATGAAATGGTCAGTCTGAATGCCAAAGGGGCTAACAATCGGGGTACGGc                                                                                                                                                                 >  2:207327/1‑238 (MQ=255)

                                                       gCATGACAATAATGACCGCTCACGCCGCCGAACCGCCTACCAATTTAGATAAACCGGAAGGGCGACTGGATATTATCGCCTGGCCGGGATACATCGAACGCGGACAAACTGATAAACAATACGACTGGGTAACG‑CAGTTCGAAAAAGAGA‑CA‑GGCT‑GCGCGGTGAA‑TGTGAAAA‑CAGC‑CGCGACTTCCGATGAAATGGTCAGCCTGATGACCAAAGGGGGTTACGACCTGGTTACGGc                                                                                                                                                                 >  1:791457/1‑238 (MQ=255)

                                                           gACAATAATGACCGCTCACGCCGCCGAACCGCCTACCAATTTAGATAAACCGGAAGGGCGACTGGATATTATCGCCTGGCCGGGATACATCGAACGCGGACCAACTGATAAACAATACGACTGGGTAACG‑CAGTTCGAAAAAGAGA‑CA‑GGCT‑GCGCGGTGAA‑TGTGAAAA‑CCGC‑CGCGACTTCCGATGAAATGGTCAGTCTGATGACCAAAGGGGGTTACGATCTGGTTACGGCATCC‑g                                                                                                                                                           >  1:1045534/1‑239 (MQ=255)

                                                              ataaatgcccccgccccccgccgAACCGCCTACAAATTTAAAAAAACGGGAAGGGGCAGTGGATCTTACCGCCGGGCAGGAAAAAGTCGAACGAGGAAAAGCTGAAAACAAATAGGACTGGTAAACG‑AAGTACGAAAAAGAGA‑CA‑GGCT‑GCGGGGGGAA‑TGGAAAAA‑CCGC‑CGCGACTTCCGATGAAATGGTCAGTCTGATGACCAAAGGGGGTTACGATCTGGTTACGGCATCC‑GGCg                                                                                                                                                        <  2:518244/222‑1 (MQ=255)

                                                                taatGACCGCTCACGCCGCCGAACCGCCTACCAATTTAGATAAACCGGAAGGGCGACTGGATATTATCGCCTGGCCGGGATACATCGAACGCGGACAAACTGATAAACAATACGACTGGGTAACG‑CAGTTCGAAAAAGAGA‑CA‑GGCT‑GCGCGGTGAA‑TGTGAAAA‑CCGC‑CGCGACTTCCGATGAAATGGTCAGTCTGATGACCAAAGGGGGTTACGATCTGGTTACGGCATCC‑GGCGAt                                                                                                                                                      >  1:175470/1‑239 (MQ=255)

                                                                taatGACCGCTCACGCCGCCGAACCGCCTACCAATTTAGATAAACCGGAAGGGCGACTGGATATTATCGCCTGGCCGGGATACATCGAACGCGGACAAACTGATAAACAATACGACTGGGTAACG‑CAGTTCGAAAAAGAGA‑CA‑GGCA‑GCGCGGCGAA‑TGTGAAAA‑CCGC‑CGCGACTGCCGATGAAATGGTCAGTCTGGTGACCAAAGGGGGTTACGATCTGGTAACGGAATCC‑gggacg                                                                                                                                                      >  2:740465/1‑235 (MQ=255)

                                                                  atGACCGCTCACGCCGCCGAACCGCCTACCAATTTAGATAAACCGGAAGGGCGACTGGATATTATCGCCTGGCCGGGATACATCGAACGCGGACAAACTGATAAACAATACGACTGGGTAACG‑CAGTTCGAAAAAGAGA‑CA‑GGCT‑GCGCGGTGAA‑TGTGAAAA‑CCGC‑CGCGACTTCCGATGAAATGGTCAGTCTGATGACCAAAGGGGGTTACGATCTGGTTACGGCATCC‑GGCGTTg                                                                                                                                                     >  1:409389/1‑238 (MQ=255)

                                                                       cGCTCACGCCGCCGAACCGCCTACCAATTTAGATAAACCGGAAGGGCGACTGGATATTATCGCCTGGCCGGGATACATCGAACGCGGACAAACTGATAAACAATACGACTGGGTAACG‑CAGTTCGAAAAAGAGA‑CA‑GGCT‑GCGCGGTGTA‑TGTGAAAA‑CCGC‑CGCGACTTCCGATGAAATGGTCAGTCTGATGACCAAAGGGGGTTACGATCTGGTTACGGCATCC‑GGCGATGCCTCGc                                                                                                                                               >  1:168379/1‑239 (MQ=255)

                                                                         cTCACGCCGCCGAACCGCCTACCAATTTAGATAAACCGGAAGGGCGACTGGATATTATCGCCTGGCCGGGATACATCGAACGCGGACAAACTGATAAACAATACGACTGGGTAACG‑CAGTTCGAAAAAGAGA‑CA‑GGCT‑GCGCGGTGAA‑TGTGAAAA‑CCGC‑CCCGACTTCCGATGAAATGGTCAGTCTGATGACCAAAGTGGGTTACGATCTGGTTACGGCATCC‑GCCGATGCCTCGCt                                                                                                                                              >  1:1074387/1‑238 (MQ=255)

                                                                          tCACGCCGCCGAACCGCCTACCAATTTAGATAAACCGGAAGGGCGACTGGATATTATCGCCTGGCCGGGATACATCGAACGCGGACAAACTGATAAACAATACGACTGGGTAACG‑CAGTTCGAAAAAGAGA‑CA‑GGCT‑GCGCGGTGAA‑TGTGAAAA‑CGCC‑CGCGACTTCCGATGAAATGGTCATGCTGAGAGCCAAAGGGGGTTACGTTCTGGTTACGGAGACC‑GGCGagacccccggcc                                                                                                                                            >  2:735742/1‑228 (MQ=255)

                                                                          tCACGCCGCCGAACCGCCTACCAATTTAGATAAACCGGAAGGGCGACTGGATATTATCGCCTGGCCGGGATACATCGAACGCGGACAAACTGATAAACAATACGACTGGGTAACG‑CAGTTCGAAAAAGAGA‑CA‑GGCT‑GCGCGGTGAA‑TGTGAAAA‑CCGC‑AGCGACTTCCGATGAAATGGTCAGTCTGATGACCAAAGGGGGTTACGATCTGGTTACGGCATCC‑GCCGATGCCTCGCAGc                                                                                                                                            >  1:999438/1‑239 (MQ=255)

                                                                             ggcccccGACCCGCCAACGAAGTTAGAAAAGCGGGGAGGGGGAATGGTTATGTTGGGCGGGCCGGGAAGAATGGAAGGCGGAAAAACGGATACCGAAGACGTAGGGAGAAAG‑GAGTGGAAAAAAGGGA‑CA‑GGGG‑GCGGGGTGAA‑GGAAAAAA‑CCGC‑CGCGTCTTCCGATGAAATGGTCAGTCTGATGACCAAAGGGGGTTACGATCTGGTTACGGCATCC‑GGCGATGCCTCGCTGCGtt                                                                                                                                         <  2:791457/238‑1 (MQ=255)

                                                                                 gccgAACCGCCTACCAATTTAGATAAACCGGAAGGGCGACTGGATATTATCGCCTGGCCGGGATACATCGAACGCGGACAAACTGATAAACAATACGCCTGGGTAACG‑CAGTTCGAAAAAGAGA‑CA‑GGCT‑GCGCGGTGAA‑TGTGAAAA‑ACGC‑CGCGACTTCCGATGAAATGGTCAGTCTGATGACCAAAGGGGGTTACGAACTGGTTACGGCATCC‑GGCGAGGCCCCGCCGCGGTTGAt                                                                                                                                     >  2:1063683/1‑239 (MQ=255)

                                                                                                    aaaaaaaaCAGGAAGGGCGACGGAATATTACCGCCGGGCGGGAAAACATCGAACGCGGAAAAACTGATAAACAATACGACTGGGAAACG‑CAGTTCAAAAAGGGGA‑CA‑GGCT‑GCGCGGGGAA‑TGTAAAAA‑CCGC‑CGCGACTTCCGATGAAATGGTCAGTCTGATGACCAAAGGGGGTTACGATCTGGTTACGGCATCC‑GGCGATGCCTCGCTGCGTTTGATTATGGGTAAACGCGTGCAg                                                                                                                  <  1:1063683/234‑1 (MQ=255)

                                                                                                       aTAAACCGGAAGGGCGACTGGATATTATCGCCTGGCCGGGATACATCGAACGCGGACAACCTGATAAACAATACGACTGGGTAACG‑CAGTTCGAAAAAGAGA‑CA‑GGCT‑GCGCGGTGAA‑TTGGAAAA‑CCGC‑CGCGACTTCCGAGGAAAGGGTCAGTCTGATGACCAACGGGGGTTCCGATCTGGTTACGGACTCCAGGCG‑GGCCGCGATGGGGTTGATTATGGGTaaaaaggggaaaccg                                                                                                               >  2:521406/1‑227 (MQ=255)

                                                                                                       aTAAACCGGAAGGGCGACTGGATATTATCGCCTGGCCGGGATACATCGAACGCGGACAAACTGATAAACAATACGACTGGGTAACG‑CAGTTCGAAAAAGAGA‑CA‑GGCT‑GCGCGGTGAA‑TGTGAAAA‑CCGC‑CGCGACTTCCGATGAAATGGTCAGTCTGATGACCAAAGGGGGTTACGATCTGGTTACGGCATCC‑GGCGATGCCTCGCTGCGTTTGATTATGGGTAAACGCGTGCAGCCg                                                                                                               >  1:1049326/1‑239 (MQ=255)

                                                                                                         agcccGGAGGGGAGACGGAATTTTGCCGCCTGGCCGGAAACCAGCAAAGGGGGAAAAACTGAAAAAAAAGCCGACGGGGAACCG‑CAGTCCAAAAAAGAGA‑CA‑GGCT‑GCGCGGTGAA‑TGGGAAAA‑CCGC‑CGCGACTTCCGATGAAATGGTCAGTCTGATGACCAAAGGGGGTTACGATCTGGTTACGGCATCC‑GGCGATGCCTCGCTGCGTTTGATTATGGGTAAACGCGTGCAGCCGAt                                                                                                             <  1:46825/236‑1 (MQ=255)

                                                                                                         aacccGGAAGGGCGATTGTATATTCACGCCGGGCGGGAATACAACGAACGGGGACAAACTGAAAAACAATCCGACGGGGTAACG‑CGGTTCGAAAAAGGGA‑CG‑GGGT‑GCGGGGTGAA‑TGTGAAAC‑CCGC‑CGCGACTTCCGATGAAATGGTCAGTCTGATGACCAAAGGGGGTTACGATCTGGTTACGGCATCC‑GGCGATGCCTCGCTGCGTTTGATTATGGGTAAACGCGTGCAGCCGAt                                                                                                             <  1:435008/236‑1 (MQ=255)

                                                                                                         aaaCCGGAAGGGCGACTGGATATTATCGCCTGGCCGGGATACATCCAACGCGGACAAACTGATAAACAATACGACTGGGTAACG‑CAGTTCGAAAAAGAGA‑CA‑GGCT‑GCGCGGTGAA‑TGTGAAAA‑CCGC‑CGCGACTTCCGATGAAATGGTCAGTCTGATGACCAAAGGGGGTTCCGTTCTGGTTACGGCATCC‑GGCGATGCCTCGCTGGGTTTGGTTATGGTGAAACGCGGGccccccct                                                                                                             >  2:1013378/1‑232 (MQ=255)

                                                                                                                aaGGGCGACTGGATATTATCGCCTGGCCGGGATACATCGAACGCGGACAAACTGATAAACAATACGACTGGGTAACG‑CAGTTCGAAAAAGAGA‑CA‑GGCT‑GCGCGGTGAA‑TGTGAAAA‑CCGC‑CGCGACTTCCGATGAAATGGTCAGTCTGATGACCAAAGGGGGTTACGAACTGGTTCAGGCATCC‑GGCGATGCCTCCCTTCGTTTGATTATGGGGAAAAGGGGGCAGCCGCTAAAAAcc                                                                                                      >  2:470684/1‑239 (MQ=255)

                                                                                                                       aCTGGATATTATCGCCTGGCCGGGATACATCGAACGCGGACAAACTGATAAACAATACGACTGGGTAACG‑CAGTTCGAAAAAGAGA‑CA‑GGCT‑GCGCGGTGAA‑TGTGAAAA‑CCGC‑CGCGACTTCCGATGAAATGGTCAGTCTGATGACCAAAGGGGGTTACGATCTGGTTACGGACTCC‑GGCGATGCCTCCCTGCGTTTGATTATGGGTAAACCCGGGCACCCGATAAAAACCCCa                                                                                                   >  2:46825/1‑235 (MQ=255)

                                                                                                                                tatCGCCTGGCCGGGATACATCGAACGCGGACAAACTGATAAACAATACGACTGGGTAACG‑CAGTTCGAAAAAGAGA‑CA‑GGCT‑GCGCGGTGAA‑TGTGAAAA‑CCGC‑CGCGACTTCCGATGAAATGGTCAGTCTGATGACCAAAGGGGGTTACGATCTGGTTACGGCATCC‑GGCGATGCCTCGCTGCGTTTGATTATGGGTAAACGCGTGCAGCCGATTAATACCGCATTGGTT‑CCCa                                                                                        >  1:429005/1‑236 (MQ=255)

                                                                                                                                tatCGCCTGGCCGGGATACATCGAACGCGGACAAACTGATAAACAATACGACTGGGTAACG‑CAGTTCGAAAAAGAGA‑CA‑GGCT‑GCGCGGTGAA‑TGTGAAAA‑CCGC‑CGCGACTTCCGATGAAATGGTCAGTCTGATGACCAAAGGGGGTTACGATCTGGTTACGGCATCC‑GGCGATGCCTCGCTGCGTTTGATTATGGGTAAACGCGTGCAACCGATTAATACCGCCTTGATT‑CCCa                                                                                        >  1:1081955/1‑236 (MQ=255)

                                                                                                                                  tCGCCTGGCCGGGATACATCGAACGCGGACAAACTGATAAACAATACGACTGGGTAACG‑CAGTTCGAAAAAGAGA‑CA‑GGCT‑GCGCGGTGAA‑TGTGAAAA‑CCGC‑CGCGACTTCCGATGAAATGGTCAGTCTGATGACCAAAGGGGGTTACGATCTGGTTACGGCATCC‑GGCGATGCCTCGCTGCGTTTGATTATGGGTAAACGCGTGCAGCCGATTAATACCGCATTGATT‑CCCAACTgg                                                                                   >  1:803806/1‑239 (MQ=255)

                                                                                                                                  tCGCCTGGCCGGGATACATCGAACGCGGACAAACTGATAAACAATACGACTGGGTAACG‑CAGTTCGAAAAAGAGA‑CA‑GGCT‑GAGCGGTGCA‑TGTGAAAA‑CCGC‑CGCGACTTCCGATGAAATGGTCAGTCTGATGACCAAAGGGGGTTACGCTCTGGTTACGGCATCC‑GGCGATGGCCACCTGCGTTTGATTTTGGGTAAACGCGTGCAGCCGATTAATACCCACTTGTTT‑CCCAAATgg                                                                                   >  2:341701/1‑239 (MQ=255)

                                                                                                                                     ccTGGCCGGGATACATCGAACGCGGACAAACTGATAAACAATACGACTGGGTAACG‑CAGTTCGAAAAAGAGA‑CA‑GGCT‑GCGCGGTGAA‑TGTGAAAA‑CCGC‑CGCGACTTCCGATGAAATGGTCAGTCTGATGACCAAAGGGGGTTACGATCTGGTTACGGCATCC‑GGCGATGCCTCGCTGCGTTTGATTATGGGTAAACGCGTGCAGCCGATTAATCACGCATTGTTT‑CCCACCTGGaaa                                                                                >  1:433174/1‑239 (MQ=255)

                                                                                                                                        ggCCGGGATACATCGAACGCGGACAAACTGATAAACAATACGACTGGGTAACG‑CAGTTCGAAAAAGAGA‑CA‑GGCT‑GCGCGGTGAA‑TGTGAAAA‑CCGC‑CGCGACTTCCGATGAAATGGTCAGTCTGATGACCAAAGGGGGTTACGATCTGGTTACGGCATCC‑GGCGATGCCTCGCTGCGTTTGATTATGGGTAAACGGGGGAAGCCGCTAAAAACCGCATTGATT‑CCAAACGGGaaaaa                                                                              >  2:528904/1‑237 (MQ=255)

                                                                                                                                           cGGGATCCATCCAACGGGGAAAAAGTGAAAAACAATGCGACGGGGTAACG‑CAGTTCGAAAAAGAGA‑CA‑GGCT‑GCGGGGTGAA‑TGTGAAAA‑CCGC‑CGCGACTTCCGATGAAATGGTCAGTCTGATGACCAAAGGGGGTTACGATCTGGTTACGGCATCC‑GGCGATGCCTCGCTGCGTTTGATTATGGGTAAACGCGTGCAGCCGATTAATACCGCATTGATT‑CCCAACTGGAAAACGCt                                                                           <  1:757799/238‑1 (MQ=255)

                                                                                                                                                   acagaacgggaaaaaacgaaaaaacaaacCGACGGGGAAACC‑AAGTTAAAAAAAGGGA‑CG‑GGCT‑GGGGGGGGAA‑TGGAAAAA‑CCGC‑CGCGACTTCCGATGAAATGGTCAGTCTGATGACCAAAGGGGGTTACGATCTGGTTACGGCATCC‑GGCGATGCCTCGCTGCGTTTGATTATGGG‑AAACGCGTGCAGCCGATTAATACCGCATTGATT‑CCCAACTGGAAAACGCTCGATCcgcgc                                                                 <  1:994882/218‑1 (MQ=255)

                                                                                                                                                    tggAACGGGGAAAAACTGAAAAAAAATACGACTGGATAACG‑AGTTTCAAAAAAGAGA‑CA‑GGCT‑GCGGGGGGAA‑TGTGAAAA‑CCGC‑CGCGACTTCCGATGAAATGGTCAGTCTGATGACCAAAGGGGGTTACGATCTGGTTACGGCATCC‑GGCGATGCCTCGCTGCGTTTGATTATGGGTAAACGCGTGCAGCCGATTAATACCGCATTGATT‑CCCAACTGGAAAACGCTCGATCcgcgc                                                                 <  1:528904/237‑1 (MQ=255)

                                                                                                                                                    tagacccgggaaaactggaaaaaaaaTCCGATGGGGTAACG‑CAGTCCAAAAAGGGGA‑CA‑GTCT‑GCGGGGGAAA‑GGGAAAAA‑CCGC‑CGCGACTTCCGATGAAATGGTCAGTCTGATGACCAAAGGGGGTTACGATCTGGTTACGGCATCC‑GGCGATGCCTCGCTGCGTTTGATTATGGGTAAACGCGTGCAGCCGATTAAGACCGCATTGATT‑CCCAACTGGAAAACGCTCGATCcgcgc                                                                 <  1:987413/219‑1 (MQ=255)

                                                                                                                                                    tCGAACGCGGACAAACTGATAAACAATACGACTGGGTAACG‑CAGTTCGAAAAAGAGA‑CA‑GGCT‑GCGCGGTGAA‑TGTGAAAA‑CCGC‑CGCGACTTCCGATGAAATGGTCAGTCTGATGACCAACGGGGGTTACGATCTGGTTACGGCATCC‑GGCGATTCCACCCTGCGTTTGGTTTTGGGTAAACGCGTGCCGCCGGTAAAAAACCACTTGTTT‑CCCACCTGGAAAACGCCCGACCcccgc                                                                 >  2:757799/1‑239 (MQ=255)

                                                                                                                                                    acGAACGCGGACAAACTGAAAAAAAAGACGACGGGATAACG‑CAGGTAGAAAAAGAGA‑CA‑GGCT‑GGGGGGTAAA‑TGTGAAAA‑CCGC‑CGCGACTTCCGATGAAATGGTCAGTCTGATGACCAAAGGGGGTTACGATCTGGTTACGGCATCC‑GGCGATGCCTCGCTGCGTTTGATTATGGGTAAACGCGTGCAGCCGATTAATACCGCATTGATT‑CCCAACTGGAAAACGCTCGATCcgcgc                                                                 <  1:959842/238‑1 (MQ=255)

                                                                                                                                                        aCGCGGACAAACTGATAAACAATACGACTGGGTAACG‑CAGTTCGAAAAAGAGA‑CA‑GGCT‑GCGCGGTGAA‑TGTGAAAA‑CCGC‑CGCGACTTCCGATGAAATGGTCAGTCTGATGACCAAAGGGGGTTACGATCTGGTTACGGCATCC‑GGCGATGc                                                                                                                                                    >  1:295735/1‑153 (MQ=255)

                                                                                                                                                                   tgtaaaaaCAACCCGAGGGGGTACCC‑GAGTCCAAAAAAGGGG‑CG‑GGGT‑GCGGGGT‑AA‑TGGGAAAAGCCGC‑CGCGACTTCCGATGAAATGGTCAGTCTGATGACCAAAGGGGGTTACGATCTGGTTACGGCATCC‑GGCGATGCCTCGCTGCGTTTGATTATGGGTAAACGCGTGCAGCCGATTAATACCGCATTGATT‑CCCAACTGGAAAACGCTCGATCCGCGCGTGGTTAAAGGCGAc                                                  <  1:698974/234‑1 (MQ=255)

                                                                                                                                                                            aaTACGACTGGGTAACG‑CAGTTCGAAAAAGAGA‑CA‑GGCT‑GCGCGGTGAA‑TGTGAAAA‑CCGC‑CGCGACTTCCGATGAAATGGTCAGTCTGATGACCAAAGGGGGTTACGATCTGGTTACGGCATCC‑GGCGATGCCTCGCTGCGTTTGATTATGGGTAAACGCGTGCAGCCGATTAATACCGCATTGATT‑CCCAACTGGAAAACGCTCGATCCGCGCGTGGTTAAAGGCGACTGGTTTAAt                                         >  1:91043/1‑239 (MQ=255)

                                                                                                                                                                              tgcGACTGGGTAACG‑AATGGCAAAAAAGAGC‑CA‑GGCT‑GCGAGGTGAA‑TGGGAAAC‑CCGG‑CGCGACTTCCGATGAAATGGTCAGTCTGATGACCAAAGGGGGTTACGATCTGGTTACGGCATCC‑GGCGATGCCTCGCTGCGTTTGATTATGGGTAAACGCGTGCAGCCGATTAATACCGCATTGATT‑CCCAACTGGAAAACGCTCGATCCGCGCGTGGTTAAAGGCGACTGGTTTAATGt                                       <  1:194717/237‑1 (MQ=255)

                                                                                                                                                                              tACGACTGGGTAACG‑CAGTTCGAAAAAGAGA‑CA‑GGCT‑GCGCGGTGAA‑TGTGAAAA‑CCGC‑CGCGACTTCCGATGAAATGGTCAGTCTGATGACCAAAGGGGGTTACGCTCTGGTTACGGCATCC‑GGCGATGCCTCGCTGCGTTTGATTTTGGGTAAACGCGGGCAGCCGGTTAATACCGTATTGCTT‑CCCACCTGGAAAATCCTCGTTCCGCGCGTGGGTAAACACCACTGGTTTAAGGt                                       >  2:250259/1‑239 (MQ=255)

                                                                                                                                                                              tACGACTGGGTAACG‑CAGTTCGAAAAAGAGA‑CA‑GGCT‑GCGCGGTGAA‑TGTGAAAA‑CCGC‑CGCGACTTCCGATGAAATGGTCAGTCTGATGACCAAAGGGGGTTACGATCTGGTTACGGCATCC‑GGCGATGCCTCGCTGCGTTTGATTATGGGTAAACGCGTGCAGCCGATTAATACCGCATTGATT‑CCCAACTGGAAAACGCTCGATCCGCGCGTGGTTAAAGGCGACTGGTTTAATGt                                       >  1:298195/1‑239 (MQ=255)

                                                                                                                                                                              tACGACTGGGTAACG‑CAGTTCGAAAAAGAGA‑CA‑GGCT‑GCGCGGTGAA‑TGTGAAAA‑CCGC‑CGCGACTTCCGATGAAATGGTCAGTCTGATGACCAAAGGGGGTTACGATCTGGTTACGGCATCC‑GGCGATGCCTCGCTGCGTTTGATTATGGGTAAACGCGTGCAGCCGATTAATACCGCATTGATT‑CCCAACTGGAAAACGCTCGATCCGCGCGGGGTTAAAGGCGACTGGTTTAATGt                                       >  1:204331/1‑239 (MQ=255)

                                                                                                                                                                              tACGACTGGGTAACG‑CAGTTCGAAAAAGAGA‑CA‑GGCT‑GCGCGGTGAA‑TGTGAAAA‑CCGC‑CGCGACTTCCGATGAAATGGTCAGTCTGATGACCAAAGGGGGTTACGATCTGGTTACGGCATCC‑GGCGATGCCTCGCTCCGTTTGATTATGGGTAAACCCGTGCAGCCCATTAATCCCGCATTGATT‑TCCAACTGAAAAAAACCCGATCCGCGCGAGGTTAAAggggcaggggttaagtt                                       >  2:919040/1‑224 (MQ=255)

                                                                                                                                                                              tACGACTGGGTAACG‑CAGTTCGAAAAAGAGA‑CA‑GGCT‑GCGCGGTGAA‑TGTGAAAA‑CCGC‑AGCGACTTCCGATGAAATGGTCAGTCTGATGACCAAAGGGGGTTACGATCTGGTTACGGCATCC‑GGCGGTGCATCGCTGCGTTTGATTATGGGTAAACGCGTGCAGCCGATTAATACCGCATTGTTT‑CCAAACTGGAAAACGGTCGATCCGCCCAGGATTAAACGCGACCGGTTAAAAGt                                       >  2:168927/1‑239 (MQ=255)

                                                                                                                                                                                cGACTGGGTAACG‑CAGTTCGAAAAAGAGA‑CA‑GGCT‑GCGCGGTGAA‑TGTGAAAA‑CCGC‑CGCGACTTCCGATGAAATGGTCAGTCTGATGACCAAAGGGGGTTACGATCTGGTTACGGCATCC‑GGCGCTGCCTCGCTTCGTTTGATTATGGGTAAACGCGGGCAGCCGCTTAATACCCCATTTATT‑CCCAACTGGAAAACGCTGGATCCCCACGGGGTTAAAGGGGACCGGTTTAttgttg                                     >  2:694514/1‑239 (MQ=255)

                                                                                                                                                                                cGACTGGGTAACG‑CAGTTCGAAAAAGAGA‑CA‑GGCT‑GCGCGGTGAA‑TGTGAAAA‑CCGC‑CGCGACTTCCGATGAAATGGTCAGTCTGATGACCAAAGGGGGTTACGATCTGGTTACGGCATCC‑GGCGATGCCTCGCTGCGTTTGATTATGGGTAAACGCGTGCAg                                                                                                                  >  2:934259/1‑163 (MQ=255)

                                                                                                                                                                                cGACTGGGTAACG‑CAGTTCGAAAAAGAGA‑CA‑GGCT‑GCGCGGTGAA‑TGTGAAAA‑CCGC‑CGCGACTTCCGATGAAATGGTCAGTCTGATGACCAAAGGGGGTTACGATCTGGTTACGGCATCC‑GGCGATGCCTCGCTGCGTTTGATTATGGGTAAACGCGTGCAg                                                                                                                  <  1:934259/163‑1 (MQ=255)

                                                                                                                                                                                cGACTGGGTAACG‑CAGTTCGAAAAAGAGA‑CA‑GGCT‑GCGCGGTGAA‑TGTGAAAA‑CCGC‑CGCGACTTCCGATGAAATGGTCAGTCTGATGACCAAAGGGGGTTACGATCTGGTTACGGCATCC‑GGCGATGCCTCGCTGCGTTTGATTATGGGTAAACGCGTGCAGCCGATTAATACCGCATTGATT‑CCCAACTGGAAAACGCTCGATCCCCGCGTGGTTAAAGGCGACTGGTTTAATGTTg                                     >  1:991064/1‑239 (MQ=255)

                                                                                                                                                                                      ggTAACG‑CAGTTCGAAAAAGAGA‑CA‑GGCT‑GCGCGGTGAA‑TGTGAAAA‑CCGC‑CGCGACTTCCGATGAAATGGTCAGTCTGATGACCAACGGGGGGTCCGATCTGGTTACGGCATCC‑GGCGATGCCTCGCTGCGTTTGATTATGGGTAAAAGCGTGCCGCCGATTAAAAACGCCTTTATT‑CCCAACTGGAAAAAGGCGGAACCGCCCGCGGGTAAAGGCAACGTGTTTAATGTTGCCTGCa                               >  2:511932/1‑239 (MQ=255)

                                                                                                                                                                                      ggTAACG‑CAGTTCGAAAAAGAGA‑CA‑GGCT‑GCGCGGTGAA‑TGTGAAAA‑CCGC‑CGCGACTTCCGATGAAATGGTCAGTCTGATGACCAAAGGGGGTTACGATCTGGTTACGGCATCC‑GGCGATGCCTCGCTGCGTTTGATTATGGGTAAACGCGTGCAGCCGATTAATa                                                                                                        >  1:206569/1‑167 (MQ=255)

                                                                                                                                                                                      ggTAACG‑CAGTTCGAAAAAGAGA‑CA‑GGCT‑GCGCGGTGAA‑TGTGAAAA‑CCGC‑CGCGACTTCCGATGAAATGGTCAGTCTGATGACCAAAGGGGGTTACGATCTGGTTACGGCATCC‑GGCGATGCCTCGCTGCGTTTGATTATGGGTAAACGCGTGCAGCCGATTAATACCGCATTGATT‑CCCACCTGGAAAAGGCTCGATCGGCAGGTGGTTAAAGGCGACTGGTTTAAGTTTGGCGGCa                               >  1:755014/1‑239 (MQ=255)

                                                                                                                                                                                      ggTAACG‑CAGTTCGAAAAAGAGA‑CA‑GGCT‑GCGCGGTGAA‑TGTGAAAA‑CCGC‑CGCGACTTCCGATGAAATGGTCAGTCTGATGACCAAAGGGGGTTACGATCTGGTTACGGCATCC‑GGCGATGCCTCGCTGCGTTTGATTATGGGTAAACGCGGGCAGCCGATTAATACCGCATTTATT‑CCCAACTGGAAAACCCTCGATCCGCCCGTGGTTAAAGGGGACCGGTTTAATGTGGGGGGCa                               >  2:441542/1‑239 (MQ=255)

                                                                                                                                                                                        taaacgaattccGAAAAACAGA‑AG‑GGGT‑GCGC‑GTGAATTGGAAAAA‑CCGC‑CGCGACTTCCGATGAAATGGTCAGTCTGATGACCAAAGGGGGTTACGATCTGGTTACGGCATCC‑GGCGATGCCTCGCTGCGTTTGATTATGGGTAAACGCGTGCAGCCGATTAATACCGCATTGATT‑CCCAACTGGAAAACGCTCGATCCGCGCGTGGTTAAAGGCGACTGGTTTAATGTTGGCGGCa                               <  1:694514/227‑1 (MQ=255)

                                                                                                                                                                                                 ttCGAAAAAGAGA‑CA‑GGCT‑GCGCGGTGAA‑TGTGAAAA‑CCGC‑CGCGACTTCCGATGAAATGGTCAGTCTGATGACCAAAGGGGGTTACGATCTGGTTACGGCATCC‑GGCGATGCCTCGCTGCGTTTGATTATGGGTAAAc                                                                                                                          >  1:153982/1‑139 (MQ=255)

                                                                                                                                                                                                 ttCGAAAAAGAGA‑CA‑GGCT‑GCGCGGTGAA‑TGTGAAAA‑CCGC‑CGCGACTTCCGATGAAATGGTCAGTCTGATGACCAAAGGGGGTTACGATCTGGTTACGGCATCC‑GGCGATGCCTCGCTGCGTTTGATTATGGGTAAACGCGTGCAGCCGATTAATACCGCATTGAtt                                                                                             >  1:405381/1‑168 (MQ=255)

                                                                                                                                                                                                 ttCGAAAAAGAGA‑CA‑GGCT‑GCGCGGTGAA‑TGTGAAAA‑CCGC‑CGCGACTTCCGATGAAATGGTCAGTCTGATGACCAAAGGGGGTTACGATCTGGTTACGGCATCC‑GGCGATGCCTCGCTGCGTTTGATTATGGGTAAACGCGTGCAGCCGATTAATACCGCATTGATT‑CCCAACTGGAAAACGCTCGATCCGCCCGTGGTTAAAGGCGACTGGTTTAATTTTTGCGGCAAAGTTTACg                      >  1:830499/1‑238 (MQ=255)

                                                                                                                                                                                                 ttCGAAAAAGAGA‑CA‑GGCT‑GCGCGGTGAA‑TGTGAAAA‑CCGC‑CGCGACTTCCGATGAAATGGTCAGTCTGATGACCAAAGGGGGTTACGATCTGGTTACGGCATCC‑GGCGATGCCTCGCTGCGTTTGATTATGGGTAAACGCGTGCAGCCGATTAAATCCGCATTGATTCCCCAACT‑GAAAACCCCCGGCCCGCGACGGGGTAAAGGCGAACGGTTTAAAGATGGAGGGAAATGTTACgg                     >  2:533519/1‑239 (MQ=255)

                                                                                                                                                                                                  tcaaaaaaGTGA‑CG‑GGGG‑GCCGGGTGAA‑T‑TGAAAA‑CCCCGCGCGAGTTACGGATAAAGTGTCAGGCTGATTGCCAAAGGGGGTAACGCTCTGGATACGGCATCC‑GGCAATGCCTCACTGCGTTTGATTATGGGGAAACCGGTGCAGCCGATTAATACCGCAGTGATT‑CCCAACTGGAAAACGCTCGATCCGCGCGTGGTTAAAGGCGACTGGTTTAATGTTGGCGGCAAAGTTTACgg                     <  2:999438/235‑1 (MQ=255)

                                                                                                                                                                                                    tcaaaaaaggagcacggttcggcgGTAAA‑TGGGAAAC‑CCGC‑CGCGACTTCCGATGAAATGGTCAGTCTGATGACCAAAGGGGGTTACGATCTGGTTACGGCATCC‑GGCGATGCCTCGCTGCGTTTGATTATGGGTAAACGCGTGCAGCCGATTAATACCGCATTGATT‑CCCAACTGGAAAACGCTCGATCCGCGCGTGGTTAAAGGCGACTGGTTTAATGTTGGCGGCAAAGTTTACgg                     <  1:376583/218‑1 (MQ=255)

                                                                                                                                                                                                    aaaaaaGAGA‑CG‑GGTG‑GGGCGGTGAA‑TGTGAAAA‑CCGC‑CGCGACTTCCGATGAAATGGTCAGTCTGATGACCAAAGGGGGTTACGATCTGGTTACGGCATCC‑GGCGATGCCTCGCTGCGTTTGATTATGGGTAAACGCGTGCAGCCGATTAATACCGCATTGATT‑CCCAACTGGAAAACGCTCGATCCGCGCGTGGTTAAAGGCGACTGGTTTAATGTTGGCGGCAAAGTTTACGGca                   <  1:740465/237‑1 (MQ=255)

                                                                                                                                                                                                     gaaaaaatgcacgggCT‑GCGCGGTAAA‑TGTGAAAC‑CCGC‑CGCGACTTCCGATGAAATGGTCAGTCTGATGACCAAAGGGGGTTACGATCTGGTTACGGCATCC‑GGCGATGCCTCGCTGCGTTTGATTATGGGTAAACGCGTGCAGCCGATTAATACCGCATTGATT‑CCCAACTGGAAAACGCTCGATCCGCGCGTGGTTAAAGGCGACTGGTTTAATGTTGGCGGCAAAGTTTACGGca                   <  1:470684/226‑1 (MQ=255)

                                                                                                                                                                                                     cgaaaaaggacttgggt‑GCGGGGGGAA‑TGTGAAGA‑CCGG‑CGCTGCTTCCGATGAAACGGGCAGTCGGATGACCAAAGGGGGTTAGGATCGGGTTCCGGCAGCC‑GGCGATGCCTCGCTGCGTTTGATTATGGGTAAACGCGTGCAGCCGATTAATACCGCAGTGATT‑CCCAACTGGAAAACGCTCGATCCGCGCGTGGTTAAAGGCGACTGGTTTAATGTTGGCGGCAAAGTTTACGGca                   <  2:1045534/223‑1 (MQ=255)

                                                                                                                                                                                                     aaaaaGAGA‑CA‑GGCT‑GCGGGGTAAA‑TGTGAAAA‑CCGC‑CGCGACTTCCGATGAAATGGTCAGTCTGATGACCAAAGGGGGTTACGATCTGGTTACGGCATCC‑GGCGATGCCTCGCTGCGTTTGATTATGGGTAAACGCGTGCAGCCGATTAATACCGCATTGATT‑CCCAACTGGAAAACGCTCGATCCGCGCGTGGTTAAAGGCGACTGGTTTAATGTTGGCGGCAAAGTTTACGGcaca                 <  1:735742/239‑1 (MQ=255)

                                                                                                                                                                                                      aaaaaagggaagaggggggggggTGAG‑TGGAAAAC‑CCGC‑CGCGACTTCCGATGAAATGGTCAGTCTGATGACCAAAGGGGGTTACGATCTGGTTACGGCATCC‑GGCGATGCCTCGCTGCGTTTGATTATGGGTAAACGCGTGCAGCCGATTAATACCGCATTGATT‑CCCAACTGGAAAACGCTCGATCCGCGCGTGGTTAAAGGCGACTGGTTTAATGTTGGCGGCAAAGTTTACGGca                   <  2:803806/218‑1 (MQ=255)

                                                                                                                                                                                                       aaaaaagcgacgtggcgtggggagAA‑TGTGAAAC‑CCGG‑CTGGTCTTCCGGTTAAAGGGGCAGTCTGATGACCAAAGGGGGTTACGTTCTGGTACCGGAATCC‑GGCGATGCCTCGCTGCGTTTGATTATGGGTAAACGCGTGCAGCCGATTTATACCGCATTGATT‑CCCAACTGGAAAACGCTCGATCCGCGCGTGGTTAAAGGTGACCGGTTTAATGTTGGCGGCAAAGTTTACGGca                   <  2:1081955/215‑1 (MQ=255)

                                                                                                                                                                                                        aaGGGA‑CAGGGCT‑GCGGGGTAAA‑TGTAAAAC‑CCGC‑CGCGACTTCCGATGAAATGGTCAGTCTGATGACCAAAGGGGGTTACGATCTGGTTACGGCATCC‑GGCGATGCCTCGCTGCGTTTGATTATGGGTAAACGCGTGCAGCCGATTAATACCGCATTGATT‑CCCAACTGGAAAACGCTCGATCCGCGCGTGGTTAAAGGCGACTGGTTTAATGTTGGCGGCAAAGTTTACGGCACAcc               <  1:974536/239‑1 (MQ=255)

                                                                                                                                                                                                                        cgcgGTGAA‑TGTGAAAA‑CCGC‑CGCGACTTCCGATGAAATGGTCAGTCTGATGACCAAAGGGGGTTACGATCTGGTTACGGCATCC‑GGCGATGCCTCGCTGCGTTTGATTATGGGTAAACGCGTGCAGCCGATTAATACCGCATTGATT‑CCCAACTGGAAAACGCTCGATCCGCGCGTGGTTAAAGGCGACTGGTTTAATGTTGGCGGCAAAGTTTACGGCACACCTTCCCAATggggg  >  1:735850/1‑238 (MQ=255)

                                                                                                                                                                                                                        cgcgGTGAA‑TGTGAAAA‑CCGC‑CGCGACTTCCGATGAAATGGTCAGTCTGATGACCAAAGGGGGTTACGATCTGGTTACGGCATCC‑GGCGATGCCTCGCTGCGTTTGATTATGGGTAAACGCGTGCAGCCGATTAATACCGCATTGATT‑CCCAACTGGAAAACGCTCGATCCGCGCGTGGTTAAAGGCGACTGGTTTAATGTTGGCGGCAAAGTTTACGGCACACCTTACCAATggggg  >  1:125664/1‑238 (MQ=255)

CAGGAGTAAGACCTTATGAGCAAGACATTTGCCCGCAGCAGCCTGTGTGCGCTCAGCATGACAATAATGACCGCTCACGCCGCCGAACCGCCTACCAATTTAGATAAACCGGAAGGGCGACTGGATATTATCGCCTGGCCGGGATACATCGAACGCGGACAAACTGATAAACAATACGACTGGGTAACG‑CAGTTCGAAAAAGAGA‑CA‑GGCT‑GCGCGGTGAA‑TGTGAAAA‑CCGC‑CGCGACTTCCGATGAAATGGTCAGTCTGATGACCAAAGGGGGTTACGATCTGGTTACGGCATCC‑GGCGATGCCTCGCTGCGTTTGATTATGGGTAAACGCGTGCAGCCGATTAATACCGCATTGATT‑CCCAACTGGAAAACGCTCGATCCGCGCGTGGTTAAAGGCGACTGGTTTAATGTTGGCGGCAAAGTTTACGGCACACCTTACCAATGGGGG  >  NC_000913/1511639‑1512088

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A