Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F17 I0 R1 160 98.0 2214160 89.0% 1970602 222.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,749,742 A→G 17.4% E171G (GAG→GGG)  sgbU → putative L‑xylulose 5‑phosphate 3‑epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,749,7420AG17.4% 156.8 / 2.7 70E171G (GAG→GGG) sgbUputative L‑xylulose 5‑phosphate 3‑epimerase
Reads supporting (aligned to +/- strand):  ref base A (27/28);  new base G (5/9);  total (32/38)
Fisher's exact test for biased strand distribution p-value = 5.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTGGAGATTATGGATACCGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCG‑ATCCACCTGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAGGCTGCGTCGATTTCGTTGGCATCTTCAAAACGCTGCATAAACTGAACTATCG  >  NC_000913/3749510‑3749976
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                            
ttaagagccaggAGTTAAGCTATTCGCCTGGCTCCCGTTCTCGGCATTCGCCCCACCCAGCCTGCAGGTTAGGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTGGAGATTATGGATACCGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCt                                                                                                                                                                                                                                       <  2:504293/228‑1 (MQ=255)
cgGGAAATCATGGGTAAAGCGTTTCGCCTGGCGTGCGATCTCGGCATTCGCACCCTCCAGGTGGCAGGTAATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTGGAGATTATGGATACCGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCt                                                                                                                                                                                                                                       <  2:956018/239‑1 (MQ=255)
 gggaaaagagtggtaaaagggttCGGCTGGGGCGGGAGTTCGGAAGTCGACCCAGCCAGCTGGCAAGTGTTGACGTCTAATACGAAGGCCACGACGAAGGCCCCCGGCAAAGTTTGGCGGAAGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTGGAGATTATGGATACCGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAG‑GGGACGAG‑ATGCt                                                                                                                                                                                                                                       <  2:245614/217‑1 (MQ=255)
       tCATGAGTAAAGCGATTCGCCTGGCGGGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTGGAGATTATGGATACCGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCa                                                                                                                                                                                                                                <  1:92780/239‑1 (MQ=255)
       tCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTGGAGATTATGGATACCGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCa                                                                                                                                                                                                                                <  1:1076400/239‑1 (MQ=255)
       tCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTGGAGATTATGGATACCGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCa                                                                                                                                                                                                                                <  1:10043/239‑1 (MQ=255)
           gAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTGGAGATTATGGATACCGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGt                                                                                                                                                                                                                            <  1:301646/239‑1 (MQ=255)
               aaaGCGATTCGGCTGGCGCGCGATCCGGGCATTCGCACCCACCGGGTGGCAGGTTATGGCGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTGGAGATTATGGATACCGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGTGGtt                                                                                                                                                                                                                        <  2:758587/239‑1 (MQ=255)
                 caggtttCGCCTGGCGGCGGATCTCGGCTATCCCCCCATCCAGCGGGCAGGTTGTGACGTCTAATACGAAGACCACGACGAAGGCACCCGGCAACGTTGTGCTGAAGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTGGAGATTATGGATACCGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGTGGtt                                                                                                                                                                                                                        <  2:112504/232‑1 (MQ=255)
                 aGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTGGAGATTATGGATACCGCGGTTATGAAATCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGACCCCCTCACCGGGGTTc                                                                                                                                                                                                                       >  1:454549/1‑238 (MQ=255)
                   ctaTTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGGCGAAGGCACACGGCAACGTTTTGCTGAAGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTGGAGATTATGGATACCGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGTGGTTCAcc                                                                                                                                                                                                                    <  1:554354/237‑1 (MQ=255)
                     aTTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTGGGGATTATGGAAACCGCGGTTATGAACTCCATCCGCAAAT‑GGAAAAAAT‑GGGACAGG‑TAGCTCGCCCCACCGCGGTTTACCg                                                                                                                                                                                                                   >  1:416810/1‑238 (MQ=255)
                      ttCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTGGAGATTATGGGTACCGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGGG‑ATGCTCGCCTCACCCTGGTTCACCGt                                                                                                                                                                                                                  >  1:758587/1‑238 (MQ=255)
                      ttCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTGGAGATTATGGATACCGCGTTTATGAACTCCCTTCGCCAAT‑GGGAAAAAT‑GGGGCGGG‑ATGCTCGCCTCACCg                                                                                                                                                                                                                             >  1:504293/1‑227 (MQ=255)
                             ggCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTGGAGATTATGGATACCGCGGTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACTAG‑ATGCTCGCCTCACCGGGGTTCACCGTCTACCCgg                                                                                                                                                                                                          >  1:716646/1‑239 (MQ=255)
                               gggcggATCTTGGCATTCGCGCCCACCAGCGGGCAGGTTATGAGGTTTATTACGAAGGCCACGGAGAAGGCCCCCGGCAACGTTTGGCTGAAGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGGAATGCTGGCGGTGGAGATTATGGATACCGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGa                                                                                                                                                                                                         <  2:204087/233‑1 (MQ=255)
                               cgcgcgATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTGGGGATTATGGATACCCCGTTTTTGAACTCCCTCAGCAAAA‑GGAAAAAAT‑GGGGCGGG‑GTGCTCGCCTAACCGTGGTTCACCGTCTaa                                                                                                                                                                                                              >  1:112504/1‑232 (MQ=255)
                                             aTTCGCCCCAGCCCGCTGGCAGGTTATGGCGTTTATTACGAAGGCCACGACGAAGGCCCCCGGCAACGTTTTGCTGAAGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTGGAGATTATGGATACCGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCa                                                                                                                                                                                           <  1:506448/238‑1 (MQ=255)
                                                cGCATCTTCCATCTGGCAGGTTATGACGTCTATTACGAAGCCCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTGGAGATT‑TGGATACCGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCAGCGcc                                                                                                                                                                                      <  1:556917/239‑1 (MQ=255)
                                                cGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTGGAGATTATGGATACCGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGTGGTTCCCCGTCTAACCGGGCGTCGGCAACCCCAgcg                                                                                                                                                                                        >  1:969125/1‑238 (MQ=255)
                                                      ctCCAGCTGGCAGGTTATGGCGTTTATTAGGAAGACCCCGAAGAAGGCACCCGGCAACGTTTTGCTGAAGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGTATAACAAGTAATGCTGGCGGTGGAGATTAGGGATACCGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGTGGTGTGCCGTCTCCCCGTACGTCGGCAACCTCAGCGCCTgggg                                                                                                                                                                                 <  2:762105/238‑1 (MQ=255)
                                                               gCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGGGGAGATTATGGAAACCGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGGCGAG‑ATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGCCACCTCAGCGCCTGGGGGAATTATGt                                                                                                                                                                        >  1:877409/1‑239 (MQ=255)
                                                                    ttATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTGGAGATTATGGATACCGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGTGGtt                                                                                                                                                                                                                        <  1:579593/186‑1 (MQ=255)
                                                                     ttgggcttttttttcGAAGGCCCCGGCGGAGGCCCCCGGAACCGTTTTGCGGAGGGGCG‑GGCGTGGGACGTGAAACAGGGGGGGGACACAAAAGTAAGGCGGGGGGTGGAAATTAGGGATCCCGGGTTTATGAACTCCATCAGCAAAG‑GGAAAAAAT‑GGGACGAG‑ATGCGCGCCCCACCGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCAGCGCCTGGGGCAATGATGTTCccgc                                                                                                                                                                  <  2:877409/225‑1 (MQ=255)
                                                                             cTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTGGAGATTATGGATACCGCGTTTATGAACTCCATCAGCAAAT‑GGGAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGTGGTTCACCGTCTACCCCGACGTCCGCAACCTCAGCGCCCGGGGCCATGAGGTTCCCGCCGAACTGa                                                                                                                                                          >  1:1104732/1‑239 (MQ=255)
                                                                             cTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTGGAGATTATGGATACCGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGCCGGCAACCCCAGCGCCCGGGGCAATGAAGTTTCCCCCCAACTGa                                                                                                                                                          >  1:125145/1‑239 (MQ=255)
                                                                              tATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTGGAGATTATGGATACCGCGTTTATGAACTCCATCAGCAAAT‑GGGAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGCCGGCAACCTAAACCCCTGGGGCAATGATGTTCCCGccgaaccgaa                                                                                                                                                         >  1:1017102/1‑239 (MQ=255)
                                                                                ttccGAAGCCCCCGCCGAAGGCCCCGGGCAACTTTTTGCGGAGGGGGT‑GGGGGGGGCAGTCGAAAAGGCGGCGGCATCAAAAGTAATGCTGGCGGTGGAGATTAGGGATACCGCGTTTATGAACCCCATCAGAAAAG‑GGAAAAAAG‑GGGACGAG‑ATGCTCGCCTCACCGTGGTTCACCGTCTCCCCGGACGTCGGCAACCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAAc                                                                                                                                                       <  2:1085662/236‑1 (MQ=255)
                                                                                ttACGAAGGCCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTGGAGATTATGGATACCGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGTGGTTCACCGt                                                                                                                                                                                                                  <  1:755737/180‑1 (MQ=255)
                                                                                        aCCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTGGAGATTATGGATACCGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑TGGACGAG‑ATGCTCGCCTCAcc                                                                                                                                                                                                                              >  2:458777/1‑160 (MQ=255)
                                                                                        aCCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTGGAGATTATGGATACCGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCAcc                                                                                                                                                                                                                              <  1:458777/160‑1 (MQ=255)
                                                                                        aCCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTGGAGATTATGGATACCGCGGTTATGAACTCCCTCAGCAAAT‑GGGAAAAAT‑GGGACGGG‑ATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCAGCGCCCGGGGCAATGAGGTTCCCGCCCAACTGaaaacgggacat                                                                                                                                               >  1:164578/1‑230 (MQ=255)
                                                                                        aCCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCT‑GGCGTGGGCAGGCGAACAGGCGGCGGCATCACAAGTAATTCTGGCGGGGGAGATTATCGAGTCCGCGTGTATTACATCCCACCGCAAAT‑GTCAAAAAG‑TGGACGAA‑GTGCCTGACTCACACTCGATGCGCCACAACCCGCAAGTCGGCAACCTCCGCCCCTGGGGCGAGGATGGTCCCCCCGacattgaacggggcctt                                                                                                                                               >  2:772121/1‑223 (MQ=255)
                                                                                         ccccgggggagggccccgcggaCGTTTTGGGGGAGGGGTGGGGGTGGGCGGTCGAACAGGCGGTGATAACCAACGGAGTGCTGGGGGTGGAGATTAGGGATACCGCGTTTATGAACTCCATCAGAAAAG‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCACGGGGGTTCACCGTCTCCCCGGACGTCGGAATCCTAAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCAtt                                                                                                                                               <  2:547179/218‑1 (MQ=255)
                                                                                            cgacgaAGGCACCCGGCAACGTTTTGCTGAAGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTGGAGATTATGGATACCGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCAGCCCCTGGGGCAATGATGTTCCCGCCGAACTGCAAATGGGCCTTGAcc                                                                                                                                           >  1:959940/1‑239 (MQ=255)
                                                                                                 aaGGCCCCCGGCAACGTTTTGCTGAAGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTGGAGATTATGGATACCGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCAGCGcc                                                                                                                                                                                      <  1:924851/191‑1 (MQ=255)
                                                                                                      ccccGGCAAGTTTTTGCGGAGGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGCATCAAAAGTAAGGCTGGCGGTGGAGATTAGGGATACCGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATcgccg                                                                                                                                  <  1:875233/237‑1 (MQ=255)
                                                                                                           ggccctttttGCTGGGGGGTC‑GGCGGGGGGAGGCGAACGGGGGGCGGCATCACAATTAATGGGGGGGGTGGAGATTATGGATACCGCGTTTATCAACTCGATCATCAAAT‑GGAAAAAGT‑GGGACGGG‑ATGCTCCCCTCACCGTGGTTCACCGTCTCCCCGGAGGTCGGCACCCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCG‑ATcc                                                                                                                           <  2:292301/233‑1 (MQ=255)
                                                                                                                    ttcgcgaggggggtggggggggAGTCGAATGGGCGGGGACCTAACAAGGTAGGCGGGGGGTGGAGATTAGGGGACCCGCGTTTATGAACGCCGTCAGCAAAT‑GGAAAAAAG‑GGGACGGG‑CTGCGCGCCTCCCGGTGGTTCACCGCCTACCCGGACGGCGGCAACCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCG‑ATCCACCTGaaa                                                                                                                   <  2:783630/221‑1 (MQ=255)
                                                                                                                        gagggggt‑ggggtGGGGGTTCTAACGGGGGGCGGCATCACAAGTAGTGCGGGGGGTGGAGATTATGGACACCGCGTTTATGAACTCCATCAGAAAAG‑GGAAAAAGT‑GGGCCGAG‑ATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCG‑ATCCACCTGAAAGaca                                                                                                               <  2:959940/235‑1 (MQ=255)
                                                                                                                          aGGGCG‑GGGGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTGGAGATGATGGATACCGCGTTTATGACCTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCAGCGCCTGGGGAAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCG‑ATCCACCTGAAAGACAcc                                                                                                             <  1:196616/238‑1 (MQ=255)
                                                                                                                                       ggCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGGGGGGATTGTGGGTAACGCGGTTATGAAATCCCTCAGGCAAT‑GGGAAAAAT‑GGGGCGAG‑ATGGTCGCCTCACCGTGGTTCACCGTCTCCCCGCACGTAGGCCACCCCCGACCCCGGGGCAATGATGTTCCCCCCGAACCGCAAATGGGGATTGACCCTAACGCCCCC‑AGCCCCCCCAAAAACACCCCACAAGTCACCg                                                                                                >  2:959829/1‑239 (MQ=255)
                                                                                                                                            tggAACGGGGGGGGGTATCAAAAGTAAGGGGGGGGGGGGAAATTGGGGACACCGTTTTTAAGACCCCCAGC‑GAAAATGGGAAAAAAG‑GGGACGAG‑AGCCTCGCCTCACCGGGTTTCACCGTCTCCCCGGACGCCGGCAACCTCAGCCCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCG‑ATCCACCTGAAAGACACCCAGCCAGTCACCGGGCa                                                                                            <  2:125145/236‑1 (MQ=255)
                                                                                                                                             caaaCGGGCGGGGGCAACACAAGTTTGGCTGGGTGGGGAAATTTTGGAACCCGCGTTTATCAACCCCCTCAG‑AAAT‑GGGAAAAAA‑GGGGCGGGTGTGCCCCCCTCACCGTGGTTCCCCGTCTACCCGGACCTCGGCACCCTCAGCGCCTGGGGCATTGATGTCCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCG‑ATCCACCTGAAAGACACCCAGCCAGTCACCGGGCa                                                                                            <  2:969125/235‑1 (MQ=255)
                                                                                                                                                              cacaAGTAATGCTGGCGGTGGAGATTATGGATACCGCGGTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCCCCGCCTGGGGGAATGATGTTCCCGCCGAACTGAAACTGAGCATCGACAGGATCG‑CGCGCATCACCCCTAAAAACACCCCGCCAAGAACCCGGGAAAGTCCCGGAAAGATcgcg                                                                         >  2:113943/1‑236 (MQ=255)
                                                                                                                                                                           ggCGGTGGAGATTATGGATACCGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGc                                                                                                                                                                                                  <  2:612156/106‑1 (MQ=255)
                                                                                                                                                                           ggCGGTGGAGATTATGGATACCGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGc                                                                                                                                                                                                  >  1:612156/1‑106 (MQ=255)
                                                                                                                                                                                 ggAGATTATGGATCCCGCGTTTTTGCACGCCATCCGAAAAT‑GGAAAAAAT‑GGGCTGAG‑ATGCTCGCCTCCCCGTGGTTCACCGTCTACCCGGACGTCGGCCACCCCAGCGCCTGGGGCAAGGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTAGCGCCGCG‑ATTCCCCTGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAg                                                      <  2:416810/239‑1 (MQ=255)
                                                                                                                                                                                 ggAGATTATGGATACCGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGCAACCCCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGCATCGCCGCG‑ATCCCCCTGAAAGACACCCAGCCAGGCACCGGGCAAAGTCCCGGACCGTTCCGCGACGTGCCGTTTGGCGAAg                                                      >  1:48481/1‑239 (MQ=255)
                                                                                                                                                                                     aTTAGGGATCCCGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCG‑ATCCACCTGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCg                                                         <  1:1070266/232‑1 (MQ=255)
                                                                                                                                                                                          ggATACCGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACCGAAACTGGGCATTGCCCGTATCGCCGCG‑ATCCCCCTGAAAGACACCCAGCCAGCCACCGGGCAAAGCCCCGGCCAGTTCCGCACCGAGCCGTTTGGAGAAgggcgcgcgg                                             >  2:253286/1‑231 (MQ=255)
                                                                                                                                                                                            aTACCGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCG‑ATCCACCTGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGGTTGGCGAAGGCTGCGTCGAt                                           >  1:482343/1‑239 (MQ=255)
                                                                                                                                                                                              aCCGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCG‑ATCCACCTGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGa                                                                                  >  1:852122/1‑198 (MQ=255)
                                                                                                                                                                                               ccGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCCTTGACCGTATCGCCCCG‑ACCCACCTGAAAGACACCCAGCCCGTCACCGGGACAAGTCCCGGACAGTTCCGCGACGTGCCGGTTGGCGAAGGCTgggcccggg                                          >  2:1070266/1‑229 (MQ=255)
                                                                                                                                                                                                cgcgTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCG‑ATCCACCTGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGCCGTGCCGTTTGGCGAAGGCTGCGTCGATTTCg                                       >  1:501321/1‑239 (MQ=255)
                                                                                                                                                                                                ccggTTTAAGAACCCCAACAGCAAAG‑TGAAAAAAG‑GGGACGGG‑ATCCCGCCCTCCCCGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCAGCGCCTGGGGCAATGATTTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCG‑ATCCACCTGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAGGCTGCGTCGATTTCg                                       <  1:91548/236‑1 (MQ=255)
                                                                                                                                                                                                  cgTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCG‑ATCCACCTGAAAGACACCCAGCCAGTCACCGGGCAAAGCCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAgg                                                     >  1:824637/1‑223 (MQ=255)
                                                                                                                                                                                                            ccccAACAGAAAAT‑GGAAAAAAT‑GGGAAGGT‑GTGCTCGCCTCACCGTGGTTCACGGTCTACCCGGGCGTCGGCAACCTCAGCGCCGGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCG‑ATCCACCTGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAGGCTGCGTCGATTTCGTTGGCATCTTCa                           <  1:1069035/237‑1 (MQ=255)
                                                                                                                                                                                                            cTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCG‑ATCCACCTGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAGGCTGCGTCGATTTCGTTGGCATCTTCa                           >  1:1105253/1‑239 (MQ=255)
                                                                                                                                                                                                               cATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCG‑ATCCACCTGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAGGCTGCGTCGATTTCGTTGGCATCTTCaaa                         >  1:564771/1‑238 (MQ=255)
                                                                                                                                                                                                                aTCAGCAAAT‑GGAAAAAAT‑GGGCCGGG‑AGGCTCGCCCCCCCGTGGTTCCCCGTCTACCCGGACGTCGGCAACCTCAGCGCCTGGGGCAATGATTTCCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCG‑ATCCACCTGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAGGCTGCGTCGATTTCGTTGGCATCTTCAAAAc                       <  1:512456/239‑1 (MQ=255)
                                                                                                                                                                                                                  ctccccacatgAAAAAATGGGGACGGA‑CTGCCCCCCCCCCCGGGGTCCGCCCTCCCCCCGGACGTGGGCACCCTCAGCGCTGGGGGCAATGTGGTCCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCG‑ATCCACCTGAAAGACCCCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAGGCTGCGTCGATTTCGTTGGCATCTTCAAAAc                       <  2:1079421/229‑1 (MQ=255)
                                                                                                                                                                                                                     cAAAT‑GGAAAAAAT‑GGGCCGGA‑ATGCCCGCCCCACCGTGGTTCCCCGTCTACCGGGACGGCGGCAACCTCAGCGCCTGGGCAAATGATTTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCG‑ATCCACCTGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAGGCTGCGTCGATTTCGTTGGCATCTTCAAAACGCTGc                  <  1:1070445/239‑1 (MQ=255)
                                                                                                                                                                                                                       aagagaaaaaaaT‑GGCGCGCG‑GTCCTCGCCTCCCGGTTGTTCCGCGTCTCCCCGGACGGCGGCACCCCCAGCGCCTGGGGCAATGATGCCCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCG‑ATCCACCTGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAGGCTGCGTCGATTTCGTTGGCATCTTCAAAACGCTGCa                 <  2:454549/233‑1 (MQ=255)
                                                                                                                                                                                                                       aaT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCG‑ATCCACCTGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAGGCTGCGTCGATTTCGTTGGCATCTTCAAAACGCTGCAt                >  1:1091935/1‑239 (MQ=255)
                                                                                                                                                                                                                         t‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCG‑ATCCACCTGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAGGCTGCGTCGATTCCGTTGGCACCTTCAAAACGCTGCATaa              >  1:532623/1‑239 (MQ=255)
                                                                                                                                                                                                                            aaaaaaaG‑GGGACGGA‑ATGCTCGCCTCCCCGTGTTTCCCGGTCTCCCCGGGCGGCGGCACCCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCG‑ATCCACCTGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAGGCTGCGTCGATTTCGTTGGCATCTTCAAAACGCTGCATaaa             <  1:187870/237‑1 (MQ=255)
                                                                                                                                                                                                                                      ggACGAG‑ATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCG‑ATCCACCTGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAGGCTGCGTCGATTTCGTTGGCATCTTCAAAACGCTGCCTAAACTGAACTAt    >  1:449911/1‑238 (MQ=255)
                                                                                                                                                                                                                                       gACGAG‑ATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCG‑ATCCACCTGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAGGCTGCGTCGATTTCGTTGGCATCTTCAAAACGCTGCATAAACTGAACTATc   >  1:277048/1‑238 (MQ=255)
                                                                                                                                                                                                                                       gACGAG‑ATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCG‑ATCCACCCGAACGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCCCGAGGTCCCGGTTGGCGAAGGCCGCGCGGGTTTCGTTGGAATCTTCcaaaacccgcaaaacataaaatatcg  >  2:27405/1‑217 (MQ=255)
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                            
CGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCT‑GGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTGGAGATTATGGATACCGCGTTTATGAACTCCATCAGCAAAT‑GGAAAAAAT‑GGGACGAG‑ATGCTCGCCTCACCGTGGTTCACCGTCTACCCGGACGTCGGCAACCTCAGCGCCTGGGGCAATGATGTTCCCGCCGAACTGAAACTGGGCATTGACCGTATCGCCGCG‑ATCCACCTGAAAGACACCCAGCCAGTCACCGGGCAAAGTCCCGGACAGTTCCGCGACGTGCCGTTTGGCGAAGGCTGCGTCGATTTCGTTGGCATCTTCAAAACGCTGCATAAACTGAACTATCG  >  NC_000913/3749510‑3749976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A