Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F28 I0 R1 140 172.9 3477528 97.9% 3404499 226.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,782,912 A→C 22.0% V250G (GTG→GGG)  gpsA ← glycerol‑3‑phosphate dehydrogenase (NAD+)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,782,9120AC22.0% 166.7 / 12.9 112V250G (GTG→GGG) gpsAglycerol‑3‑phosphate dehydrogenase (NAD+)
Reads supporting (aligned to +/- strand):  ref base A (46/38);  new base C (9/18);  total (55/57)
Fisher's exact test for biased strand distribution p-value = 7.60e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 112 total aligned reads displayed.

TCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTG‑GCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCG‑GTTACGCGACTG‑G‑TTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAAT‑AACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCA  >  NC_000913/3782685‑3783149
                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                
tCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTG‑GCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCG‑GTTACGCCACTG‑G‑TTGTCGGTACCAGTAAGCACCAAATCGccc                                                                                                                                                                                                                                       >  2:32318/1‑238 (MQ=255)
tCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTG‑GCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCG‑GGTACGCCACCG‑G‑TTGTTGGTACCAGGAAGCACCAGATCGcc                                                                                                                                                                                                                                        >  2:1129551/1‑238 (MQ=255)
  aaTGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTG‑GCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCG‑GTGATGCGCCGA‑G‑TTGTCGGACAAGGTAAGTCCCAGATCGCCaa                                                                                                                                                                                                                                      >  2:325317/1‑238 (MQ=255)
    tgctgcCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTG‑GCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCG‑GTTACGCAACAG‑G‑TTGTCGGTGCAGGGAAGCACCAGACCGCCaacc                                                                                                                                                                                                                                    >  2:799593/1‑236 (MQ=255)
         ccTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTG‑GCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCG‑GTTTCCCCCCTG‑G‑TTGTCGCTACAGGTTAGCACCacacaccccaaccccccc                                                                                                                                                                                                                              >  1:91101/1‑222 (MQ=255)
          cTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTG‑GCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTCCCCCCCCCTGACCAATCTTCCCCTGCGCGCTTTGTACATCCAGGCCCGGACCGAGCATCATGCCAAAACGGCG‑GTTACGCGACTG‑G‑TTGTCGGTACAGGAAAGCACCAGACCGCCAAGCCCCGCCa                                                                                                                                                                                                                             <  1:1022941/239‑1 (MQ=255)
           tCGCGCGCGTTTTTTCCGCAATATAATCCTTGATAAATTTCCTCGTTTATGG‑GCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCCCCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCG‑GTTACGCGACTG‑G‑TTGTCGGTACAGGTAAGCCCCAGACCGCCAAGCCCCGCCAt                                                                                                                                                                                                                            <  1:1447933/239‑1 (MQ=255)
           tCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTG‑GCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCG‑GTTACGCGACTG‑G‑TTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCAt                                                                                                                                                                                                                            <  1:840038/239‑1 (MQ=255)
           tCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTG‑GCATTTCAACGCCGAAGCGAGGCGCCAGTTCGCGGACTTCTTTCGTATGGCGGTAACCTCCCACCCCCTGACCAATCTCCTCCTGCGCGCTTTGTACACCCATGCCCTGACAGAGCATCATGCAAAAACGGCG‑GTTACGCGACTG‑G‑TTGTCGGAACAGGTAAGCCCCAGATCGCCAAGCCCCGCCAt                                                                                                                                                                                                                            <  1:444633/239‑1 (MQ=255)
           ccgcgcgcgTTTTTCCCCCAATATAAGACTTGATAAATTTCCTCGGTTATTG‑GCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCCCCCCCTGCCCAACCTTCTCCTGCGCGCTTTGTACATCCATGCCCGGACCGAGCATCATGCCAAAACGGCG‑GTTACGCGACTG‑G‑TTGTCGGTACAGGTAAGCCCCAGATCGCCAAGCCCCGCCAt                                                                                                                                                                                                                            <  1:31216/238‑1 (MQ=255)
                     tttttCCGAAATATAATACTTGATAAATTTCCTCGGTTATTG‑GCATTTCAACGCCGAAGCGATGCGCCAGTGCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCCCCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCG‑GTTACGCGACTG‑G‑TTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAg                                                                                                                                                                                                                  <  2:1103289/239‑1 (MQ=255)
                               ttataATACTTGAT‑AATTTTCTCGGTTTGTGTGCATTTCAACGCAGAGGCGATGCGCTAGGTCGCGGAATTCTTTCGTATTGCGGTATCCCTCCCCCCCCAGACCAATCTCCTCCTGCGCGTTTTGTACACCCGTGCCCTGACCGAGAAACAGGCAAAACCGGCG‑GTTTCGCGCATG‑G‑TTGCGGGTAGTGGTAAGCTCCAGAGCGCCAGGCCCCGCCATGCCCATAAAGGTGGCAGGGt                                                                                                                                                                                                        <  1:1297952/238‑1 (MQ=255)
                               ttaaaataCTGTATAAATTCCCCCGGTTTTTG‑CCATTTCAACCCCGAACCGATGCGCCAGTTCGCGGCCTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGCCCAACCTCCCCCTGCGCGCTTTGTCCATCCATGCCCTGACCGAGCATCATGCCAAAACGGCG‑GTTCCGCGACTG‑G‑TTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGt                                                                                                                                                                                                        <  1:1373151/235‑1 (MQ=255)
                                 ataataCTGAATAAATTTCCTCGGTTGTTG‑GCATTTCAAGGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTGCTTATTGCGGTGGCCTTCCCCCACCTGACCAATCTTCTCCTGCGCGCTTTGTACACCCAGGCCCTACCCGAGCATCAGGCAAAAACGGCG‑GTTACGGGACTG‑G‑TTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCg                                                                                                                                                                                                      <  1:1467852/239‑1 (MQ=255)
                                  taataCTTGATAAATTCCCTCGGTTATTG‑GCATTCCAACGCCGAAGCAATGCGCCAGTTCGCGGCCTTCTTTCGTATTGCGGTAGCCTTCCCCCCCCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCG‑GTTACGCGACTG‑G‑TTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGc                                                                                                                                                                                                             <  1:522394/231‑1 (MQ=255)
                                     taCTTGATAAATTTCCTCGGTTATTG‑GCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCG‑GTTACCCGACTG‑G‑GTGTCGGGAAAGGGAAGCACCAGAAAGGCCACCCCCGCCCAGCCCCCAAAAGGGGCAGGGtgcgca                                                                                                                                                                                                   >  1:173986/1‑233 (MQ=255)
                                        ttGATAAATTTCCTCGGTTATTG‑GCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCG‑GTTACGCGACTG‑G‑TTGTCGGTCCAGGGCAACACCAGATCGCCCAACCCCCCCCTGCCCCCAAACGAGGCAGGGGCGGGAccc                                                                                                                                                                                                >  1:287821/1‑238 (MQ=255)
                                        ttGATAAATTTCCTCGGTTATTG‑GCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCG‑GTTACGCGACCG‑GTTTGTGGGT‑CAGGGAAGCACCACAACCCCCAGCCCCGCCCCGCCCCTAAAAGTGGCAGGGGCCGCCCCCa                                                                                                                                                                                               >  1:315228/1‑239 (MQ=255)
                                          gATAAATTTCCTCGGTTATTG‑GCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCG‑GTTACGCGCCCG‑G‑TTGTCGGTACACGTAAGCACCAAAACGCCCAACCCCGCCCTGCCCCTAAAAGCGGCCAGGGCGGCACCCac                                                                                                                                                                                              >  1:748956/1‑237 (MQ=255)
                                          gATAAATTTCCTCGGTTATTG‑GCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCG‑GTTACGCGACTG‑G‑TTGTCGGTCCAGGTAAGCACCAGAACGCCAAGCCCCCCCCTCCCCACACAGGTGGCAGGGTCGGCACCCAgc                                                                                                                                                                                             >  2:1347860/1‑239 (MQ=255)
                                          gATAAATTTCCTCGGTTATTG‑GCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCG‑GTTACGCGACTG‑G‑TTGTCGGTACAGGTAAGCACCAGATCGCCAAACCCCGCCATGCCCCTAAAGGGGGCAGGGGCGGCACCCAgc                                                                                                                                                                                             >  2:686547/1‑239 (MQ=255)
                                          gATAAATTTCCTCGGTTATTG‑GCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCG‑GTTACGCGACTG‑G‑TTGTCGGGACAGGTAAGCCCCAGATCGCCAAGCCCCGCCCTGCCCATAAAGGGGGCAGGGTCGGCACCCAg                                                                                                                                                                                              >  2:1418459/1‑238 (MQ=255)
                                          gATAAATTTCCTCGGTTATTG‑GCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCG‑GTTACGCGACTG‑G‑GTGTCGGTCCAGGGAAGCAACAAATCTCCAAACCCCGCCCTGCCCCAAAAGGTGGCAGGGGCCGCAcccccc                                                                                                                                                                                             >  2:1261648/1‑236 (MQ=255)
                                          gATAAATTTCCTCGGTTATTG‑GCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCG‑GTTACGCGACCG‑G‑TTGTCGGTACAGGGAAGCACCAGATCGCCAAACCCCCCCCTGCCCACACAGGTGGCAGGGGGGGCACCCac                                                                                                                                                                                              >  1:1515312/1‑237 (MQ=255)
                                          gATAAATTTCCTCGGTTATTG‑GCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCAACCCCTGACCGAGCATCATGCCAAAACGGCG‑GCTACCAGACAT‑G‑TTGGCGGGAAACGGAAAACCCAGAACCCAAAGCCCCCCCCTACCCCAAACAGTCGCATGGGCGGCAcccca                                                                                                                                                                                              >  1:1632762/1‑236 (MQ=255)
                                            tAAATTTCCTCGGTTATTG‑GCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCG‑GTTTCGCGACTG‑G‑TTGTTGGGACAGGTAAGCACCAGATCGCCCAACCCCGCCCTGCCCCTAAAGGGGGGAGGGGCggggccagtc                                                                                                                                                                                             >  2:522394/1‑229 (MQ=255)
                                            tAAATTTCCTCGGTTATTG‑GCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCG‑GTTACCCCACCC‑G‑GTGTCGGTGCAGGTGAGCCACAAATACCCCACCCCCCCCCCACCCCTACAAGTGGCAgggcgcgcccccgcag                                                                                                                                                                                            >  1:550026/1‑225 (MQ=255)
                                                       ggTTGTTG‑GCCTTTCAACGCAGAAGCGACGCTCCTGTGCGCGCGCTTCTTTCGTATGGCTGTAGCCTTCCACCCCCCGACCACTTTACTCCTGCGGGTTTTGTACGTCCATGCCCTGCCCGCGCGTCATGCAAAAACGGCG‑GTTCCGCGACGG‑G‑TTGCCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGa                                                                                                                                                                                 <  1:1018646/238‑1 (MQ=255)
                                                         ttAGTG‑GCTTTTCAGCGCCGAAGCGCTGCGCCGGTTCGCGGATTTCTTTCGTATTGCGGTAGCCCTCCCCCACCACACCACTCTCCCCCTGCGCGCTTTGTCCAGCCATGCCCTGACCGACCATCATGCCAAAACGGCG‑GGTAGGCGCCTG‑G‑TTGTCGGTACAGGTAAGCACCAGACCGCCACGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGAcg                                                                                                                                                                               <  1:409913/238‑1 (MQ=255)
                                                          tATTG‑GCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCG‑GTTCCGCGACTG‑G‑TTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGAcgcg                                                                                                                                                                             <  2:281463/239‑1 (MQ=255)
                                                          tATTG‑GCATTCCAACGCCGAAGCGATGCGCCAGTGCGCGGATTTCTTTCGTATTGCGGTAGCCTTCCCCCCCCTGACCAATCTTCCCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCG‑GTTACGCGACTG‑G‑TTGTCGGTACAGGTAAGCACCAGAGCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGAcgcg                                                                                                                                                                             <  2:472297/239‑1 (MQ=255)
                                                                  aTTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCG‑GCTACCCCGCCA‑G‑TGGTCGGGACAAGGAAGCCCCCCATCCCCCAGCCCCCCCCCGCCCCCAAAAGAGGGAGGGGCCGCACCCCCCGCCGccccccaccgccacctct                                                                                                                                                                       >  1:937185/1‑224 (MQ=255)
                                                                     tCACCGCGGAAGCGCGGGGCCAGTTGCTGGCCTGCTTTCGTATTGCGGCAGCCTTCCACCACCGGCCCAATCTCCCCCTGCGCGCTTTGTCAATCCAGGCCCTGACCGAGCAGCATGCCAAAGCGGGG‑GTTACGCGACTG‑G‑TTTCCGGTACAGGTAGGCCCCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTcagc                                                                                                                                                                   <  1:370391/239‑1 (MQ=255)
                                                                     tCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCG‑GTTACGCGACTG‑G‑TTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTcagc                                                                                                                                                                   <  2:550926/239‑1 (MQ=255)
                                                                     tCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCG‑GTTACGCGACTG‑G‑TTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTcagc                                                                                                                                                                   <  1:584588/239‑1 (MQ=255)
                                                                      aaaggcggaggagacgcgctggTTCGCGGACTTCTTACGAATTCGGTTAGCTTCCCCCCCCCTTCACACTCTTCTCCTGCGCGCTTGTTACACCATTGCCTTGCCCTCGAATCTTGCCAAGCCGGCG‑TTTCCCCGACTG‑G‑TTCTGGTTTATGGTATGCCCCGTAGCCCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTcagc                                                                                                                                                                   <  1:1220474/217‑1 (MQ=255)
                                                                          gCCGAAGCGATGCGCCAGTTCGCGGCCTTCTTTCGTATTGCGGTAGCCTCCCACCCCCGGACCAATCTTCTCCTGCGCGCTTTGTACACCCATGCCCTGCCCGAGCATCATGCCAAAACGGCG‑GTTACGCGACTG‑G‑TTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCagccag‑cc                                                                                                                                                             <  2:1507788/239‑1 (MQ=255)
                                                                            caatgcaaggccccggTTCGCGAACTTCTTTCATATTGTGTAAGCCGTCCGCCGCCAGACCAAGCTTCCCCCGCGCGCTTTGTACATCCCTGCCCTGCCATACAATCCGGCCAAAACGGCG‑GTTGCGCCCCTG‑C‑TGGTCGGTACAGGTATGCCCAAGATCGCCAAGCGCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCa                                                                                                                                                           <  1:879806/224‑1 (MQ=255)
                                                                              aaGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCG‑GATCCGCCGCTC‑G‑TTGTCTGGCCAGGGAAGAACCCAATCGCCAAACCCCGCCCACCCCCTCAAAGTGGCAGGGCCCGACCCCAGCGCCGCCCCCCGCCGCGCCACTTcagcccacccccg                                                                                                                                                          >  1:1350017/1‑231 (MQ=255)
                                                                               aGCGATGCGCCAGTTCGCGGCCTTCTTTCGTATGGCGGTAGCCTTCCCCCACCTGACCAATCTCCTCCTGCGCGCTTTGTACATCCAGGCCCTGACCGAGCATCATGCCAAAACGGCG‑GTTACGCGACTG‑G‑TTGTCGGTACAGGTAAGCCCCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACgg                                                                                                                                                        <  2:941525/239‑1 (MQ=255)
                                                                                  gaggggcgcgTTCGCGACCTTCTTTCGTATGGCGGTGGCCTTCCCCCCCCTGCCAAATCTTCTCCGGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAACCGGCG‑GTTACGCGACTG‑G‑TTGCCGGTAGAGGTAAGCACCAGACCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGt                                                                                                                                                      <  1:542595/229‑1 (MQ=255)
                                                                                        ccAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCG‑GTTACCCCACTG‑G‑TTGTCGGTACAAGGAAGCACCAAATCGCCCAACCCCCCCCAGCCCCTAAAAGGGGGAGGGGCGGGCCCCCGCGCCCGCCCAAGACGCCACAATTTAGACAGACCCACGGGGGacag                                                                                                                                                 >  1:388431/1‑235 (MQ=255)
                                                                                         gtgTTCGCGGACTTTTTTCGTAGTGCGGCAGCCTCCCACGTCCGGACCAGCCTCCCCCGGCTCACTTTGTACATCACGGCCGTCACCGGTCAAGACGCCAAACGGGGG‑TTTCCGATTCTG‑G‑TTGTCGTCACGTGAAAGCCCAAGCCCGCAACGCCCCGCCATGCCCATAAAGGGGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAgc                                                                                                                                               <  1:1129551/236‑1 (MQ=255)
                                                                                         cAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCG‑GTTACGCGACTG‑G‑TTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAgcg                                                                                                                                              <  2:1049431/239‑1 (MQ=255)
                                                                                                      tctttctgtttgcgtgcgCCTCCCCCCGCCAGCCCAATTTTCTCCTGGCCGCTTTGTGCACCCATTCCCTGACCGATATGCACGCCAAAACGGGG‑TTTAGGCGACTG‑G‑TTGGGGGTACAGTTAAGCCCCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCAtt                                                                                                                                 <  1:107739/222‑1 (MQ=255)
                                                                                                      tcttTCGTATTGCGGTAGCCTTCCCCCCCCTGACCAATCTTCCCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCG‑GTTACGCGACTG‑G‑TTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCAtt                                                                                                                                 <  1:640016/239‑1 (MQ=255)
                                                                                                      tcttTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGCCCGAGCATCATGCCAAAACGGCG‑GTTACGCGACTG‑G‑TTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCAtt                                                                                                                                 <  2:1368373/239‑1 (MQ=255)
                                                                                                      tcttACGAATTGGGGTACCCTCCCACCCCCTGACAAATTTTCACCGGCGCGCTTTGTACCTCCAGGCCCTGACCGAGCAACATCCCAAAACGGCG‑GTTACGCGATTG‑G‑TTGTCGGCACAGGAAAGCCCCAGATCCCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCAtt                                                                                                                                 <  1:206035/239‑1 (MQ=255)
                                                                                                            tttttGCGGTAGCCTTCCCCCACCTGCCCATCCTCCCCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCG‑GTTACGCGACTG‑G‑TTGTCGGTACAGGTAAGCACCAGATCCCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑cc                                                                                                                          <  1:1418459/236‑1 (MQ=255)
                                                                                                            gTATTGCGGTAGCCTTCCCCCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCG‑GTTACGCGACTG‑G‑TTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑cc                                                                                                                          <  2:748956/239‑1 (MQ=255)
                                                                                                               ttGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCG‑GTTACACGCCTG‑G‑GTGTTGGGTCCCGGAAGCAACAAAACGACGCCCCCCACCAGGCCCTTAACGGTGGCAGGGGCGGCACCCAGCGCCGCACCACGACGCGCCAATTCAGACAA‑CGCACAGGGGATCAGCGCCGGAcaggccgccgaacccga                                                                                                                        >  1:631607/1‑223 (MQ=255)
                                                                                                                            ccccccccccccaattcttccCCCGCGCGTTTTGTAACTCCAGCCCCAGCCAGGCCATAAGGCAAAAGGGGGG‑TTTGCGCGACTG‑G‑TTGGCGGTCAAGGAAACCCCCAGCCCCCCACCCCCCGCCATGCCCATAAAGGTGGCGGGGTCGGCCCCCAGCGCCGCCCCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATCCCGTCg                                                                                                          <  1:1261504/224‑1 (MQ=255)
                                                                                                                              cccaccTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGCCCGAGCATCATGCCAAAACGGCG‑GTTACGCGACTG‑G‑TTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGa                                                                                                        <  2:937185/238‑1 (MQ=255)
                                                                                                                              accaccTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCG‑GTTACGCGACTG‑G‑TTGTCGGTACAGGTAAGCCCCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGa                                                                                                        <  2:369594/239‑1 (MQ=255)
                                                                                                                               ccaccTGCCCAATCTTCCCCTGCGCGCTTTGTACACCCATGCCCTGACCGAGCATCATGCCAAAACGGCG‑GTTACGCGACTG‑G‑TTGCCGGTACAGGTAAGCACCAGACCGCAAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGAc                                                                                                       <  2:1515312/239‑1 (MQ=255)
                                                                                                                                   cTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCG‑GTTACGCGCCCG‑G‑TTGTCGGTACAGGGAAGCACCAAATCGCCAAACCCCGCCATGCCCATAAAGGTGGGAGGGGCGGCACCCCGCCCCGCACCCAGACGCCACATTTCAGCCCG‑CCCCCCGGGGGACAGCGCCCGACGCCGCTTCGCC‑CCCAAAcacaaaaccgcggccatc                                                                                                    >  1:1580676/1‑221 (MQ=255)
                                                                                                                                      aCCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCG‑GTTACGCGGCTG‑G‑TTGTCGGGACAGGGAAAGACCAAAACGCCCAGCCCCGCCCCGCCCATAAAGGGGGGCGGGGCGGGACCCAGCGCCCCACCAACACGCGACCTTTTAGCCCC‑CCCACGGGGGAGCAGCGCGGGGACCGCCTTCGCC‑CCCAAACCACCACCGCCGGACACcccc                                                                                                 >  1:811197/1‑238 (MQ=255)
                                                                                                                                      aCCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCG‑GTTACGCGCCTG‑G‑TTGTCGGTACAGGGAAGCACCAAATCGCCAAACCCCCCCCTGCCCATAAAGGGGGGAGGGGCGGCACCCCCCGCCCCCCCCAGACACCACCTTTCAGCCAG‑CCCACCGGGGCTCAGCCCCGCACGCGCATTCCCC‑CCACAAccccgaccccccgccaacccc                                                                                                 >  1:823483/1‑219 (MQ=255)
                                                                                                                                          aTCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCG‑GTTACGCGACGG‑G‑TTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGACATCCCCGCAcc                                                                                            <  2:741261/239‑1 (MQ=255)
                                                                                                                                             ttctCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCG‑GTTACGCGTCTG‑T‑TTGTCGGTACAGGTAAGCACCAGATCGCCAGGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGACATCCCCGCACCAAt                                                                                         <  2:183412/239‑1 (MQ=255)
                                                                                                                                                tcCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCG‑GTTACGCGCCTG‑G‑TTGTCGGTACAGGTAAGCACCACATCGCCAAACCCCCCCATGCCCCTAAAGATGGCAGGGTCGGGACCCAGCGCCGCCCCACAACGCGACATTTCAGCCAG‑CCCCCGGGCGATCAGCGCCGTACGCGCCTTCGCC‑CCAACACCCATCCCCCCGGACATACCCCCCCCCATGGc                                                                                      >  1:1189962/1‑239 (MQ=255)
                                                                                                                                                  cTGCGCGTTTTGTGCACCCACGCCCTGCCCGAGCAACATCCCAAAGCGGCG‑GTTACCCGCGTGTG‑TTG‑CGGTACAGGTAAGCACCAGACCCCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCaa                                                                                    <  1:1261648/239‑1 (MQ=255)
                                                                                                                                                     ctgtgttagaacctccgggCCATGACCGAGCA‑CAAACCCAAACCGCGTGTTCGGCGAGTG‑G‑TTGCGGGTACAGGTAAGCACCAGACCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCCCCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAAT‑a                                                                                 <  1:956769/220‑1 (MQ=255)
                                                                                                                                                     cgcgcTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCG‑GTTTCCCGACTG‑G‑TTGTCGGTACAGGGAAGCCCCACAACGCCAAGCCCCCCCATGCCCCTAAAGGGGGCAGGGGCGGCCCCCCCCGCCGCCCCCAAACGCGACACTTCAGCCCG‑CCCCCCGGTGATCAGCGCCCTACGCGCCTTCCCC‑CCCAAACCCACACCGTCCGACAACCCCGcccccaccgcaacca                                                                                 >  1:1296824/1‑228 (MQ=255)
                                                                                                                                                          tttGTACATCCATGCCCTGACCGGGCATCATGCCAAAACGGCG‑GTTCCGCGACTG‑G‑TTGTCGGTACAGGTAACCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGa                                                                                                                  <  2:1367048/201‑1 (MQ=255)
                                                                                                                                                            tGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCG‑GTTACGCGACTG‑G‑TTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGc                                                                                                                                                                                                             <  2:1098249/110‑1 (MQ=255)
                                                                                                                                                                 ctCCATGCCCTGACCGAGCATCATGCCAAAACGGCG‑TTTCCGCTACTG‑G‑TTGTCGGTACAGGTAACCACCAGACCGCCAGGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAAT‑AACGTTTTTCACcg                                                                    <  2:1210267/238‑1 (MQ=255)
                                                                                                                                                                  tCCAGGCCCTGACCGAGCATCATGCCAAAACGGCG‑GTTACGCGACTG‑G‑TTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAAT‑AACGTTTTTCACcgc                                                                   <  2:7026/239‑1 (MQ=255)
                                                                                                                                                                      tGCCCTGACCGAGCATCATGCCAAAACGGCG‑GTTTCGCGACTG‑G‑GTTTCGGCACAGAGAAAAACCAAAACGCCAAACCCCCCCCTTCCCATAAAGGGGGCCAGGGCGGCACCCCCCGCCGCCCCCAAACGCGCCATTTTAGCCCC‑CCCCCGGGGGGTTCACGCCGCAAGCGCATTCGCACCCAAAACCGCCA‑CGCCGGACACCCCCCCCCCCACGGCCCA‑AACCGTTTTCACCCcgcc                                                                >  1:1306131/1‑238 (MQ=255)
                                                                                                                                                                      tGCCCTGACCGAGCATCATGCCAAAACGGCG‑GTTTCCCGACTG‑G‑GTGTCGGGACAGGGAAGCCCCAAAACGCCCAGCCCCGCCCTGCCCACAAAGGTGGGAGGGGGGGCACCCCGCGCCGCACCCAGACGCGACATTTCAGTCCG‑CCCACGCGTGACCAGCGCCCTACGCGCAGTCGCC‑CCAAAAACGAAAACCGCGGAAACCCCCCCACCACCTGCAATAAACGttttcaaccccac                                                                 >  1:940292/1‑229 (MQ=255)
                                                                                                                                                                           ggACCGGGCATCAGGCCAAAACGGCG‑GTTACGCGACTG‑G‑TTGTCGGTACAGGTAAGCCCCAGACCCCCCAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAAT‑AACGTTTTTCACCGCGCCGCCAAg                                                          <  1:686547/238‑1 (MQ=255)
                                                                                                                                                                             aCCGAGCATCATGCCAAAACGGCG‑GTTACGCGACTG‑G‑GTGTCGGTACAGGGAAGCCACAAAACGCCAAACCCCCCCACGCCCATAAAGGGGGCAGGGGCGGCACCCCGCGCCCCCCCCAGACCCCACATTTCCGCCAG‑CCCCCCGGGGATCAGCGCCCCACCCCCACTCCCA‑CCAAAACCGCAACCCCCGGCACTCCCCCCACCCATGGCCAA‑AAAGCTTTTCACCCCCCCCCCaaca                                                         >  1:422204/1‑236 (MQ=255)
                                                                                                                                                                              ccaggCATCAGCCAAAAACGGCG‑GTTCCGCGACTG‑T‑TTGTCGGTACGGGAAAGCCCCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAAT‑AACGTTTTTCACCGCGCCGCCAAGCTg                                                       <  2:1433297/235‑1 (MQ=255)
                                                                                                                                                                                gggCATCAGGCCAAAACGGCG‑GTTACGTGACTG‑G‑TTGCCGGTACAGGAAAGCACCAGACCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAAT‑AACGTTTTTCACCGCGCCGCCAAGCTGCa                                                     <  2:1306131/237‑1 (MQ=255)
                                                                                                                                                                                   catcatGCCAAAACGGCG‑GTTACGCGACTG‑G‑TTTTCGGGACAGGGAAGCACCAAAACGCCCAACCCCCCCATGCCCCCAAAGGGGGGAGGGGCGGCACCCAGCCCCGCACCCAAACGCCACCATTTAGCCAG‑CCCACCGGGGATCAGCGCCGCACCCCCCTTCGCA‑CCAAAACCCAAACCGCCCGACATCCCCCCCCCCAAAGGAAT‑AAAGTTTTTTCCCCCgccccccacaccgccg                                                   >  1:714395/1‑228 (MQ=255)
                                                                                                                                                                                    atcagGCCAAGACGGCG‑TTTACGCGACTG‑G‑TAGTCGCTAGAGGTAAGCACCACGTCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGACCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGACAGCCCCGCACCAATGGCAAT‑AACGTTTTTCACCGCGCCGCCAAGCTGCACGc                                                  <  1:1544992/238‑1 (MQ=255)
                                                                                                                                                                                    ataatGCCAAACCGGGG‑GTTCCGCGCCTG‑T‑TTGCCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAAT‑AACGTTTTTCACCGCGCCGCCAAGCTGCACGc                                                  <  2:91101/235‑1 (MQ=255)
                                                                                                                                                                                    accatGCCAAAAGGGCG‑GTGACGCGCCGG‑G‑TTGTCGTTACGGGTAAGCACCAGACCGCCAGGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAAT‑AACGTTTTTCACCGCGCCGCCAAGCTGCACg                                                   <  2:1287826/235‑1 (MQ=255)
                                                                                                                                                                                                  gcg‑gTTCCGCGACTG‑G‑TTGGCGGTACAGGTAAGCACCAGACCGCCAACCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAAT‑AACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGa                                    <  1:1614182/238‑1 (MQ=255)
                                                                                                                                                                                                          cgcgCCTG‑G‑TGGCCGGTACAGTTAAGCCCCAGACCCACAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAAT‑AACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTa                            <  2:388431/239‑1 (MQ=255)
                                                                                                                                                                                                                      tgtgGGTACAGGTACGCCCCAGATCGCCCAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAAT‑AACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGaa                   <  2:614074/238‑1 (MQ=255)
                                                                                                                                                                                                                            tACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAAT‑AACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACtttt             >  2:8953/1‑238 (MQ=255)
                                                                                                                                                                                                                              cAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAAT‑AACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGcc          >  2:1221127/1‑239 (MQ=255)
                                                                                                                                                                                                                               aGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGAcgcg                                                                                                                                                                             <  1:833833/78‑1 (MQ=255)
                                                                                                                                                                                                                               aGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGAcgcg                                                                                                                                                                             >  2:833833/1‑78 (MQ=255)
                                                                                                                                                                                                                               aGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAAT‑AACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGcc          >  2:1390119/1‑238 (MQ=255)
                                                                                                                                                                                                                                 gTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAAT‑AACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCa       >  2:293689/1‑239 (MQ=255)
                                                                                                                                                                                                                                 gTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCACTCGCA‑CCAAAACCGATACCGTCGGACATCCCCGCACAAATGGCAAT‑AACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTACACGCGGCAACTTTTGccccc       >  1:254815/1‑236 (MQ=255)
                                                                                                                                                                                                                                  tAAGCACCAGACCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAAT‑AACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCg         <  2:80808/236‑1 (MQ=255)
                                                                                                                                                                                                                                    aGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAAT‑AACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGt     >  2:1087891/1‑238 (MQ=255)
                                                                                                                                                                                                                                      cACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCACT‑CACGTTTTTCACCGCGCCGCCAAGCTGCCCGCCCATGACATCCGGATTGCTGTAAACTCGGAACCTTTTGCCGCAGTgc   >  1:552120/1‑238 (MQ=255)
                                                                                                                                                                                                                                      cACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAAT‑AACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGCTTGCTGTAAACGCGGAAACTTTTGCCGCCGTgca  >  1:1245573/1‑239 (MQ=255)
                                                                                                                                                                                                                                      cACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAAT‑AACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTgca  >  2:1223339/1‑239 (MQ=255)
                                                                                                                                                                                                                                      cACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAAT‑AACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTgca  >  2:1250502/1‑239 (MQ=255)
                                                                                                                                                                                                                                      cACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAAT‑AACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTgca  >  2:946404/1‑239 (MQ=255)
                                                                                                                                                                                                                                      cACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAAT‑AACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTgca  >  1:1548081/1‑239 (MQ=255)
                                                                                                                                                                                                                                      cACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAAT‑AACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTgca  >  2:1502381/1‑239 (MQ=255)
                                                                                                                                                                                                                                      cACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAAT‑AACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTgca  >  2:370052/1‑239 (MQ=255)
                                                                                                                                                                                                                                      cACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAAT‑AACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTgca  >  2:251632/1‑239 (MQ=255)
                                                                                                                                                                                                                                      cACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAAT‑AACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTgca  >  1:767670/1‑239 (MQ=255)
                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                
TCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTG‑GCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCG‑GTTACGCGACTG‑G‑TTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAAT‑AACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCA  >  NC_000913/3782685‑3783149

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A