Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F111 I0 R1 46 91.4 1555988 99.0% 1540428 264.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,948,449 (T)7→6 100% coding (1520/1521 nt) yifB ← magnesium chelatase family protein and putative transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,948,4430T.51.4% 206.4 / 276.0 105intergenic (+20/+5)yifE/yifBUPF0438 family protein/magnesium chelatase family protein and putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (24/27);  new base . (26/28);  total (50/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

Only 100 of 106 total aligned reads displayed.

GTGATTTCACCATCAAAGAGGCACAACTGCTTGAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGTATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCCTTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAATATCAGCAATGGTTCGTGCAACTTTCAATAACCGCTGCCAGGCACGAATCGATAACCCCAGATGGATCAGTGTTCCTTCCAACCACATCGCATCTTCGCTCTCAAGTTTGCAGAATTGGCGTATTTCCGGACTATCCAGCCAGGCATTCAGTTTATTCTGCCGCTTAAATTG  >  NC_000913/3948158‑3948720
                                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                     
gTGATTTCACCATCAAAGAGGCACAACTGCTTGAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGTATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCCtttt                                                                                                                                                                                                                                                                                    >  1:727855/1‑289 (MQ=255)
    tttCACCATCAAAGAGGCACAACTGCTTGAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGTATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCCTTTTTTTa                                                                                                                                                                                                                                                                                >  1:12823/1‑289 (MQ=255)
              aaaGAGGCACAACTGCTTGAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGTATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCCTTTTTTTATGTCagtagt                                                                                                                                                                                                                                                                      <  2:524926/289‑1 (MQ=255)
                      acaACTGCTTGAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGTATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCCTTTTTTTATGTCAGTAGTTTCTGCa                                                                                                                                                                                                                                                               <  1:14147/288‑1 (MQ=255)
                      acaACTGCTTGAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGTATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCC‑TTTTTTATGTCAGTAGTTTCTGCAga                                                                                                                                                                                                                                                             <  1:237701/289‑1 (MQ=255)
                           tGCTTGAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGTATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCCTTTTTTTATGTCAGTAGTTTCTGCAgatgga                                                                                                                                                                                                                                                         <  2:727855/289‑1 (MQ=255)
                             cTTGAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGTATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCC‑TTTTTTATGTCAGTAGTTTCTGCAGATGGATGa                                                                                                                                                                                                                                                      >  2:725332/1‑289 (MQ=255)
                                gAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGTATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCCTTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGc                                                                                                                                                                                                                                                    <  1:350515/289‑1 (MQ=255)
                                  gCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGTATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCC‑TTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCaa                                                                                                                                                                                                                                                  >  2:468746/1‑288 (MQ=255)
                                     tCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGTATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCCTTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACg                                                                                                                                                                                                                                               >  1:703811/1‑289 (MQ=255)
                                     tCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGTATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCCTTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACg                                                                                                                                                                                                                                               >  1:5289/1‑289 (MQ=255)
                                                   tCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGTATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCCTTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCaaa                                                                                                                                                                                                                                                 <  1:202312/273‑1 (MQ=255)
                                                   tCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGTATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCCTTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCaaa                                                                                                                                                                                                                                                 >  2:202312/1‑273 (MQ=255)
                                                    cAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGTATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCCTTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATa                                                                                                                                                                                                                                >  1:532384/1‑289 (MQ=255)
                                                    cAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGTATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCCTTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATa                                                                                                                                                                                                                                >  2:493289/1‑289 (MQ=255)
                                                    cAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGTATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCCTTTTTTTATGTCAGTAGTTTCTGCAGAGGGATGAGCAAACGGTCAATCGCTCGATa                                                                                                                                                                                                                                >  2:288428/1‑289 (MQ=255)
                                                    cAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGTATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCC‑TTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATa                                                                                                                                                                                                                                >  1:41597/1‑288 (MQ=255)
                                                    cAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGTATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCC‑TTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAg                                                                                                                                                                                                                               >  1:283523/1‑289 (MQ=255)
                                                               aTCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGTATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCC‑TTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGcct                                                                                                                                                                                                                     <  1:556325/288‑1 (MQ=255)
                                                                   tGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGTATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCCTTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGcctcct                                                                                                                                                                                                                  >  2:714622/1‑288 (MQ=255)
                                                                   tGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGTATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCC‑TTTCTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGc                                                                                                                                                                                                                >  1:188988/1‑289 (MQ=255)
                                                                         aCGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGTATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCCTTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAAt                                                                                                                                                                                                            >  2:634900/1‑288 (MQ=255)
                                                                          cgcgAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGTATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCCTTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGc                                                                                                                                                                                                          <  1:637880/289‑1 (MQ=255)
                                                                           gcgAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGTATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCC‑TTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTg                                                                                                                                                                                                        <  2:515954/289‑1 (MQ=255)
                                                                             gAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGTATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCCTTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTg                                                                                                                                                                                                        <  1:542034/288‑1 (MQ=255)
                                                                              aGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGTATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCC‑TTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGAc                                                                                                                                                                                                      <  1:725332/288‑1 (MQ=255)
                                                                              aGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGTATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCC‑TTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACg                                                                                                                                                                                                     >  2:404027/1‑289 (MQ=255)
                                                                                    ttACCGAGGAAGAGAAACTCTTCGTAGCAGTATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCC‑TTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGa                                                                                                                                                                                                <  2:738424/288‑1 (MQ=255)
                                                                                    ttACCGAGGAAGAGAAACTCTTCGTAGCAGTATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCC‑TTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGa                                                                                                                                                                                                <  1:468746/288‑1 (MQ=255)
                                                                                    ttACCGAGGAAGAGAAACTCTTCGTAGCAGTATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCC‑TTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGa                                                                                                                                                                                                <  2:283523/288‑1 (MQ=255)
                                                                                    ttACCGAGGAAGAGAAACTCTTCGTAGCAGTATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTAGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCC‑TTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGa                                                                                                                                                                                                <  2:504425/288‑1 (MQ=255)
                                                                                    ttACCGAGGAAGAGAAACTCTTCGTAGCAGTATGCCGTGGCGAACGGGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCC‑TTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGa                                                                                                                                                                                                <  1:11192/288‑1 (MQ=255)
                                                                                     tACCGAGGAAGAGAAACTCTTCGTAGCAGTATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCCTTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGa                                                                                                                                                                                                <  2:5289/288‑1 (MQ=255)
                                                                                           ggAAGAGAAACTCTTCGTAGCAGTATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTGCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCCTTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTc                                                                                                                                                                                         >  1:84865/1‑289 (MQ=255)
                                                                                             aaGAGAAACTCTTCGTAGCAGTATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCC‑TTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCt                                                                                                                                                                                                                                     <  2:371133/242‑1 (MQ=255)
                                                                                             aaGAGAAACTCTTCGTAGCAGTATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCC‑TTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCt                                                                                                                                                                                                                                     >  1:371133/1‑242 (MQ=255)
                                                                                                   aaCTCTTCGTAGCAGTATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCCTTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGAt                                                                                                                                                                                 <  2:629174/289‑1 (MQ=255)
                                                                                                   aaCTCTTCGTAGCAGTATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTCCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCCTTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGAt                                                                                                                                                                                 <  2:609729/289‑1 (MQ=255)
                                                                                                      tctTCGTAGCAGTATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCC‑TTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAat                                                                                                                                                                             <  1:259976/289‑1 (MQ=255)
                                                                                                      tctTCGTAGCAGTATGCCGTGGCGAACGGGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCC‑TTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAat                                                                                                                                                                             <  1:11410/289‑1 (MQ=255)
                                                                                                       ctTCGTAGCAGTATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCC‑TTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAata                                                                                                                                                                            >  2:507427/1‑289 (MQ=255)
                                                                                                                aGTATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCCTTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAATATCAGCa                                                                                                                                                                      <  2:398529/287‑1 (MQ=255)
                                                                                                                aGTATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCCTTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAATATCAGCa                                                                                                                                                                      <  2:532384/287‑1 (MQ=255)
                                                                                                                  tATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCC‑TTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAATATCAGCAATGGt                                                                                                                                                                 >  1:572940/1‑289 (MQ=255)
                                                                                                                    tGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCC‑TTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAATATCAGCAATGGt                                                                                                                                                                 >  1:599450/1‑287 (MQ=255)
                                                                                                                    tGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCC‑TTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAATATCAGCAATGGt                                                                                                                                                                 <  2:599450/287‑1 (MQ=255)
                                                                                                                         tGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCCTTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAATATCAGCAATGGTTCGTGc                                                                                                                                                           >  2:316812/1‑289 (MQ=255)
                                                                                                                          ggCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCCTTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAATATCAGCAATGGTTCGTGCa                                                                                                                                                          >  2:705900/1‑289 (MQ=255)
                                                                                                                            cGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCCTTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAATATCAGCAATGGTTCGTGCaa                                                                                                                                                         <  1:634900/288‑1 (MQ=255)
                                                                                                                            cGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCCTTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAATATCAGCAATGGTTCGTGCaa                                                                                                                                                         <  1:400659/288‑1 (MQ=255)
                                                                                                                                cGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCC‑TTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAATATCAGCAATGGTTCGTGCAACTTTCa                                                                                                                                                   >  2:38910/1‑289 (MQ=255)
                                                                                                                                    aGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCC‑TTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAATATCAGCAATGGTTCGTGCAACTTTCAATa                                                                                                                                                >  2:506514/1‑288 (MQ=255)
                                                                                                                                      ccAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCC‑TTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAATATCAGCAATGGTTCGTGCAACTTTCAATAAc                                                                                                                                              <  2:572940/288‑1 (MQ=255)
                                                                                                                                           gACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCC‑TTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAATATCAGCAATGGTTCGTGCAACTTTCAATAACCGCTGc                                                                                                                                        <  1:618097/289‑1 (MQ=255)
                                                                                                                                                          cgTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCC‑TTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCATACTGATCAATATCAGCAATGGTTCGTTCAAGTTTCAATAACCGCTGCCAGGCACGAATCGAt                                                                                                                         >  2:363661/1‑289 (MQ=255)
                                                                                                                                                          cgTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCC‑TTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAATATCAGCAATGGTTCGTGCAACTTTCAATAACCGCTGCCAGGCACGAATCGAt                                                                                                                         >  1:566804/1‑289 (MQ=255)
                                                                                                                                                           gtgtGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCCTTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAATATCAGCAATGGTTCGTGCAACTTTCAATAACCGCTGCCAGGCACGAATCGa                                                                                                                          <  1:724845/288‑1 (MQ=255)
                                                                                                                                                           gtgtGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCCTTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAATATCAGCAATGGTTCGTGCAACTTTCAATAACCGCTGCCAGGCACGAATCGa                                                                                                                          <  2:703811/288‑1 (MQ=255)
                                                                                                                                                             gtgGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCCTTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAATATCAGCAATGGTTCGTGCAACTTTCAATAACCGCTGCCAGGCACGAATCGATaa                                                                                                                       <  2:178738/289‑1 (MQ=255)
                                                                                                                                                              tgGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCC‑TTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAATATCAGCAATGGTTCGTGCAACTTTCAATAACCGCTGCCAGGCACGAATCGATAAc                                                                                                                      >  2:523761/1‑288 (MQ=255)
                                                                                                                                                              gtgaaaacgttttggACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCCTTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAATATCAGCAATGGTTCGTGCAACTTTCAATAACCGCTGCCAGGCACGAATCGATaa                                                                                                                       >  1:473138/15‑288 (MQ=255)
                                                                                                                                                               ggTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCC‑TTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAATATCAGCAATGGTTCGTGCAACTTTCAATAACCGCTGCCAGGCACGAATCGATAAcc                                                                                                                     <  1:507427/288‑1 (MQ=255)
                                                                                                                                                                   cAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCC‑TTTCTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAATATCAGCAATGGTTCGTGCAACTTTCAATAACCGCTGCCAGGCACGAATCGATAACCCCAGa                                                                                                                <  2:188988/289‑1 (MQ=255)
                                                                                                                                                                    aagaagatGACGCGCATTAAGCGTTCAAAACGTTGTCACACCCTGTCCAGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTAAAACGATTCTGACGATTAATAAAAAGGGCTGAATGCCCTTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAATATCAGCAATGGTTCGTGCAACTTTCAATAACCGCTGCCAGGCACGAATCGATAACCCCAGa                                                                                                                <  1:559789/283‑1 (MQ=255)
                                                                                                                                                                          atGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCCTTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAATATCAGCAATGGTTCGTGCAACTTTCAATAACCGCTGCCAGGCACGAATCGATAACCCCAGATGGATc                                                                                                          >  1:479905/1‑289 (MQ=255)
                                                                                                                                                                          atGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCCTTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAATATCAGCAATGGTTCGTGCAACTTTCAATAACCGCTGCCAGGCACGAATCGATAACCCCAGATGGATc                                                                                                          <  1:714622/289‑1 (MQ=255)
                                                                                                                                                                           tGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCC‑TTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAATATCAGCAATGGTTCGTGCAACTTTCAATAACCGCTGCCAGGCACGAATCGATAACCCCAGATGGATCAg                                                                                                        >  1:297152/1‑289 (MQ=255)
                                                                                                                                                                                cgTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCC‑TTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAATATCAGCAATGGTTCGTGCAACTTTCAATAACCGCTGCCAGGCACGAATCGATAACCCCAGATGGATCAGTGtt                                                                                                    >  2:248819/1‑288 (MQ=255)
                                                                                                                                                                                 gTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCCTTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAATATCAGCAATGGTTCGTGCAACTTTCAATAACCGCTGCCAGGCACGAATCGATAACCCCAGATGGATCAGTGtt                                                                                                    >  2:612107/1‑288 (MQ=255)
                                                                                                                                                                                 gTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCC‑TTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAATATCAGCAATGGTTCGTGCAACTTTCAATAACCGCTGCCAGGCACGAATCGATAACCCCAGATGGATCAGTGttc                                                                                                   <  2:41597/288‑1 (MQ=255)
                                                                                                                                                                                 gTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCC‑TTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAATATCAGCAATGGTTCGTGCAACTTTCAATAACCGCTGCCAGGCACGAATCGATAACCCCAGATGGATCAGTGttc                                                                                                   <  2:297152/288‑1 (MQ=255)
                                                                                                                                                                                          gTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCC‑TTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAATATCAGCAATGGTTCGTGCAACTTTCAATAACCGCTGCCAGGCACGAATCGATAACCCCAGATGGATCAGTGTTCCTTCCAAc                                                                                           <  1:523761/287‑1 (MQ=255)
                                                                                                                                                                                             cAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCC‑TTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAATATCAGCAATGGTTCGTGCAACTTTCAATAACCGCTGCCAGGCACGAATCGATAACCCCAGATGGATCAGTGTTCCTTCCAACCACAt                                                                                      <  1:404027/289‑1 (MQ=255)
                                                                                                                                                                                                aaCGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCCTTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAATATCAGCAATGGTTCGTGCAACTTTCAATAACCGCTGCCAGGCACGAATCGATAACCCCAGATGGATCAGTGTTCCTTCCAACCACATc                                                                                     >  2:249810/1‑288 (MQ=255)
                                                                                                                                                                                                     tttCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCCTTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAATATCAGCAATGGTTCGTGCAACTTTCAATAACCGCTGCCAGGCACGAATCGATAACCCCAGATGGATCAGTGTTCCTTCCAACCACATCGCATCt                                                                               <  2:479905/289‑1 (MQ=255)
                                                                                                                                                                                                            cccTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCCTTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGAc                                                                                                                                                                                                      <  1:148408/163‑1 (MQ=255)
                                                                                                                                                                                                            cccTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCCTTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGAc                                                                                                                                                                                                      >  2:148408/1‑163 (MQ=255)
                                                                                                                                                                                                                ttCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCC‑TTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAATATCAGCAATGGTTCGTGCAACTTTCAATAACCGCTGCCAGGCACGAATCGATAACCCCAGATGGATCAGTGTTCCTTCCAACCACATCGCATCTTCGCTCTCAAGt                                                                   >  1:281960/1‑289 (MQ=255)
                                                                                                                                                                                                                    ggcggTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCCTTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAATATCAGCAATGGTTCGTGCAACTTTCAATAACCGCTGCCAGGCACGAATCGATAACCCCAGATGGATCAGTGTTCCTTCCAACCACATCGCATCTTCGCTCTCAAGTTTg                                                                <  2:152000/289‑1 (MQ=255)
                                                                                                                                                                                                                         tAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCCTTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAATATCAGCAATGGTTCGTGCAACTTTCAATAACCGCTGCCAGGCACGAATCGATAACCCCAGATGGATCAGTGTTCCTTCCAACCACATCGCATCTTCGCTCTCAAGTTTGCAGaa                                                           >  2:169398/1‑289 (MQ=255)
                                                                                                                                                                                                                              cGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCC‑TTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAATATCAGCAATGGTTCGTGCAACTTTCAATAACCGCTGCCAGGCACGAATCGATAACCCCAGATGGATCAGTGTTCCTTCCAACCACATCGCATCTTCGCTCTCAAGTTTGCAGAATTGGCg                                                     <  2:566804/289‑1 (MQ=255)
                                                                                                                                                                                                                               gCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCCTTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAATATCAGCAATGGTTCGTGCAACTTTCAATAACCGCTGCCAGGCACGAATCGATAACCCCAGATGGATCAGTGTTCCTTCCAACCACATCGCATCTTCGCTCTCAAGTTTGCAGAATTGGCg                                                     <  1:705900/289‑1 (MQ=255)
                                                                                                                                                                                                                                cAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCC‑TTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAATATCAGCAATGGTTCGTGCAACt                                                                                                                                                       <  1:188340/189‑1 (MQ=255)
                                                                                                                                                                                                                                cAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCC‑TTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAATATCAGCAATGGTTCGTGCAACt                                                                                                                                                       >  2:188340/1‑189 (MQ=255)
                                                                                                                                                                                                                                        aGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCCTTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAATATCAGCAATGGTTCGTGCAACTTTCAATAACCGCTGCCAGGCACGAATCGATAACCCCAGATGGATCAGTGTTCCTTCCAACCACATCGCATCTTCGCTCTCAAGTTTGCAGAATTGGCGTATTTCCgg                                            >  2:373771/1‑289 (MQ=255)
                                                                                                                                                                                                                                          gTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCCTTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAATATCAGCAATGGTTCGTGCAACTTTCAATAACCGCTGCCAGGCACGAATCGATAACCCCAGATGGATCAGTGTTCCTTCCAACCACATCGCATCTTCGCTCTCAAGTTTGCAGAATTGGCGTATTTCCGGa                                           <  1:169398/288‑1 (MQ=255)
                                                                                                                                                                                                                                             cTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCCTTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCCAATGCTGACGTGTGATAATGTCAGACTGATCAATATCCGCAATGGTTCGTGCAACTTTCAATAACCCCTGCCAGGCACGAATAGATAACCCCAGATGGATCAGTGTTCCTTCCAACCACATCGCATCTTCGCTCTCAAGTTTGCAGAATTGGCGTATTTCCGGACTa                                        >  1:69134/1‑288 (MQ=255)
                                                                                                                                                                                                                                                 aGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCC‑TTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAATATCAGCAATGGTTCGTGCAACTTTCAATAACCGCTGCCAGGCACGAATCGATAACCCCAGATGGATCAGTGTTCCTTCCAACCACATCGCATCTTCGCTCTCAAGTTTGCAGAATTGGCGTATTTCCGGACTATccagc                                  <  2:232481/289‑1 (MQ=255)
                                                                                                                                                                                                                                                       cacCGATTCTGACGATTAATAAAAAGGGCGAAATGCCC‑TTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAATATCAGCAATGGTTCGTGCAACTTTCAATAACCGCTGCCAGGCACGAATCGATAACCCCAGATGGATCAGTGTTCCTTCCAACCACATCGCATCTTCGCTCTCAAGTTTGCAGAATTGGCGTATTTCCGGACTATCCAGCCAGGCa                            >  2:3028/1‑289 (MQ=255)
                                                                                                                                                                                                                                                       cacCGATTCTGACGATTAATAAAAAGGGCGAAATGCCC‑TTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAATATCAGCAATGGTTCGTGCAACTTTCAATAACCGCTGCCAGGCACGAATCGATAACCCCAGATGGATCAGTGTTCCTTCCAACCACATCGCATCTTCGCTCTCAAGTTTGCAGAATTGGCGTATTTCCGGACTATCCAGCCAGGCa                            >  2:129280/1‑289 (MQ=255)
                                                                                                                                                                                                                                                          cGATTCTGACGATTAATAAAAAGGGCGAAATGCCCTTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAATATCAGCAATGGTTCGTGCAACTTTCAATAACCGCTGCCAGGCACGAATCGATAACCCCAGATGGATCAGTGTTCCTTCCAACCACATCGCATCTTCGCTCTCAAGTTTGCAGAATTGGCGTATTTCCGGACTATCCAGCCAGGCAt                           >  1:403706/1‑288 (MQ=255)
                                                                                                                                                                                                                                                            aTTCTGACGATTAATAAAAAGGGCGAAATGCCC‑TTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAATATCAGCAATGGTTCGTGCAACTTTCAATAACCGCTGCCAGGCACGAATCGATAACCCCAGATGGATCAGTGTTCCTTCCAACCACATCGCATCTTCGCTCTCAAGTTTGCAGAATTGGCg                                                     >  1:545004/1‑259 (MQ=255)
                                                                                                                                                                                                                                                            aTTCTGACGATTAATAAAAAGGGCGAAATGCCC‑TTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAATATCAGCAATGGTTCGTGCAACTTTCAATAACCGCTGCCAGGCACGAATCGATAACCCCAGATGGATCAGTGTTCCTTCCAACCACATCGCATCTTCGCTCTCAAGTTTGCAGAATTGGCg                                                     <  2:545004/259‑1 (MQ=255)
                                                                                                                                                                                                                                                               cTGACGATTAATAAAAAGGGCGAAATGCCC‑TTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAATATCAGCAATGGTTCGTGCAACTTTCAATAACCGCTGCCAGGCACGAATCGATAACCCCAGATGGATCAGTGTTCCTTCCAACCACATCGCATCTTCGCTCTCAAGTTTGCAGAATTGGCGTATTTCCGGACTATCCAGCCAGGCATTCAGttt                    >  2:55629/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                  aCGATTAATAAAAAGGGCGAAATGCCCTTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAATATCAGCAATGGTTCGTGCAACTTTCAATAACCGCTGCCAGGCACGAATCGATAACCCCAGATGGATCAGTGTTCCTTCCAACCACATCGCATCTTCGCTCTCAAGTTTGCAGAATTGGCGTATTTCCGGACTATCCAGCCAGGCATTCAGTTTAt                  >  2:141571/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                      ttAATAAAAAGGGCGAAATGCCCTTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAATATCAGCAATGGTTCGTGCAACTTTCAATAACCGCTGCCAGGCACGAATCGATAACCCCAGATGGATCAGTGTTCCTTCCAACCACATCGCATCTTCGCTCTCAAGTTTGCAGAATTGGCGTATTTCCGGACTATCCAGCCAGGCATTCAGTTTATTCTg              <  2:473138/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                gggCGAAATGCCC‑TTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATc                                                                                                                                                                                <  1:194813/116‑1 (MQ=255)
                                                                                                                                                                                                                                                                                gggCGAAATGCCC‑TTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATc                                                                                                                                                                                >  2:194813/1‑116 (MQ=255)
                                                                                                                                                                                                                                                                                   cGAAATGCCCTTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAATATCAGCAATGGTTCGTGCAACTTTCAATAACCGCTGCCAGGCACGAATCGATAACCCCAGATGGATCAGTGTTCCTTCCAACCACATCGCATCTTCGCTCTCAAGTTTGCAGAATTGGCGTATTTCCGGACTATCCAGCCAGGCATTCAGTTTATTCTGCCGCTTAAATTg  >  2:460319/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                                     aaaTGCCC‑TTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAATATCAGCAATGGTTCGTGCAACTTTCAATAACCGCTGCCAGGCACGAATCGATAACCCCAGATGGATCAGTGTTCCTTCCAACCACATCGCATCTTCGCTCTCAAGTTTGCAGaa                                                           <  2:587570/228‑1 (MQ=255)
                                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                     
GTGATTTCACCATCAAAGAGGCACAACTGCTTGAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGTATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCTTTCCGGCGGTAAACCGCAGGTTGAAGGTGCTGAAGACTACACCGATTCTGACGATTAATAAAAAGGGCGAAATGCCCTTTTTTTATGTCAGTAGTTTCTGCAGATGGATGAGCAAACGGTCAATCGCTCGATAGCTAACTGCCTCCTGCAAATGCTGACGTGTGATAATGTCAGACTGATCAATATCAGCAATGGTTCGTGCAACTTTCAATAACCGCTGCCAGGCACGAATCGATAACCCCAGATGGATCAGTGTTCCTTCCAACCACATCGCATCTTCGCTCTCAAGTTTGCAGAATTGGCGTATTTCCGGACTATCCAGCCAGGCATTCAGTTTATTCTGCCGCTTAAATTG  >  NC_000913/3948158‑3948720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A