Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F9 I0 R1 80 116.8 2384848 97.0% 2313302 226.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,098,390 A→C 18.9% Y219S (TAC→TCC)  rhaS → transcriptional activator of rhaBAD and rhaT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,098,3900AC18.9% 284.0 / 16.2 128Y219S (TAC→TCC) rhaStranscriptional activator of rhaBAD and rhaT
Reads supporting (aligned to +/- strand):  ref base A (67/33);  new base C (2/24);  total (69/59)
Fisher's exact test for biased strand distribution p-value = 4.02e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 130 total aligned reads displayed.

TTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTTCTCTTTCACTGCGTACGCTACATCGGCAGCTTAAGCAGCAAACGGGACTGACGCCTCAGCGATACCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACGCCATATTGCGAATAATCAACTTCGTTCT  >  NC_000913/4098152‑4098626
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                            
ttACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGCAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGAAAAACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGTCCATTTTGCCGATGAGGTGAATTGGGATCACGTGGCGGGTCAG‑TTTTTTCTTTGACGTCGTCCGCTACATGAGCAGGTTAAGCAGCAAACGGGCACGACGCCTCCGCGATa                                                                                                                                                                                                                                              >  1:246636/1‑239 (MQ=255)
      tCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTTCTCTTTCACTGCGTACGCTACATCGGCAGCTTAAGCAGCAAACGGGACTGACGCCTCAGCGATACCTGa                                                                                                                                                                                                                                         >  2:511200/1‑238 (MQ=255)
       cGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGAATTGAGATGCCGTGGCGGATCAA‑TTTTCTCTTTCACTGCGTACGCTACATCGGAAGCTTAAGCAGCAAACGGGACTGACGCCTCAGCGATACCTGaa                                                                                                                                                                                                                                        >  2:619059/1‑238 (MQ=255)
                                   gCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTTCTCTTTCACTGCGTACGCTACATCGGCAGCTTAAGCAGCAAACGGGACTGACGCCTCAGCGATACCTGAACCGCCTGCGACTGATGAAAGCCCGACa                                                                                                                                                                                                             >  2:1078146/1‑237 (MQ=255)
                                      aTTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTTCTCTTTCACTGCGTACGCTACATCGGCAGCTTAAGCAGCAAACGGGACTGACGCCTCAGCGATACCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGc                                                                                                                                                                                                        <  2:773141/239‑1 (MQ=255)
                                       ttACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTTCTCTTTCACTGCGTACGCTACATCGGCAGCTTAAGCAGCAAACGGGACTGACGCCTCAGCGATACCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCt                                                                                                                                                                                                       >  2:863482/1‑239 (MQ=255)
                                       ttACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTTCTCTTTCACTGCGTACGCTACATCGGCAGCTTAAGCAGCAAACGGGACTGACGCCTCAGCGATACCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCt                                                                                                                                                                                                       >  2:322560/1‑239 (MQ=255)
                                       ttACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTTCTCTTTCACTGCGTACGCTACATCGGCAGCTTAAGCAGCAAACGGGACTGACGCCTCAGCGATACCTGAACCGCCTGCGACTGATGAAAGCCCGAAATCTGCt                                                                                                                                                                                                       >  2:1143423/1‑239 (MQ=255)
                                       ttACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGCAAAACAGCGCATCACGTCTAAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTTCTCTTTCCCTGCGTACGCTCCATCCGCCGCTTAAGCGGCAAACGGGCCTGACGCCTCAGCGCTACCCGCACCGCACGCCACTGATGaacacccccccacttc                                                                                                                                                                                                        >  1:259533/1‑224 (MQ=255)
                                            gCTCTTGCTGCGTAAAAGAGGTTGGCAGGACAACCTGGAAAACGGCACAGCCGGTCTCAACTCGGTGTGGGCCTGGCTGGAAGACCATTTTGCCGATGAGGTGAAGGGGGTTGCGTTGGCGGATCAA‑TTTTCTCTTTCACTGCGTACCCTCCACCGGCTGTTTATGCAGAAACGGGGACTCACACCACAGCGATACTCGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGcc                                                                                                                                                                                                  <  1:386911/239‑1 (MQ=255)
                                               ctTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTTCTCTTTCACTGCGTACGCTACATCGGCAGCTTAAGCAGCAAACGGGACTGACGCCTCAGCGATACCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCcaca                                                                                                                                                                                               >  2:363237/1‑239 (MQ=255)
                                                 tgctgcGTAAAAGCATTTTGCAGGGAAACCTGGAAAACAGCCCATCCCGCCCAAACTTTCTTCTGGCTTGGCTGTGGGCCCATTTTGCCGATGGGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTTCTCTTTCACTGCGTACGCTACACCGGCATCTTAAGCAGCAAACGGGACTGCCCCCCCAGCAATACCTGATCCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGc                                                                                                                                                                                             <  1:1047883/239‑1 (MQ=255)
                                                 tgctgcGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTTTTCTCTCACTGCGTACGCTACATCGGCAGCTTAAGCAGCAAACGGGACTGACGCCTCAGCGATACCTTAACCGCCTGCGACTGATTACAGCCCGACATTTGCTACGCCACAGc                                                                                                                                                                                             >  1:142375/1‑239 (MQ=255)
                                                 tgctgcGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTTCTCTTTCACTGCGTACGCTACATCGGCAGCTTAAGCAGCAAACGGGACTGACGCCTCAGCGATACCTGAACCGCCTGCGACTGATGAACGCCCGCAATCTGCTACGCCACAg                                                                                                                                                                                              >  1:1135138/1‑238 (MQ=255)
                                                 tgctgcGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTTCTCTTTCACTGCGTACGCTACATCGGCAGCTTAAGCAGCAAACGGGACTGACGCCTCAGCGATACCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGc                                                                                                                                                                                             >  2:432468/1‑239 (MQ=255)
                                                      tataagagacatttGCAGGGAGACCGGAAAAACAGCGCATCCCGGTTCAACTTGCTTCGGGGCGGGCTGGGGGACCTTTTTGCCCATGAGGTGAATTGGGATGCCGTGGCGGGTCAA‑TTTTCCTTTTCCCGGCGGACGCTACATCGGCAGCTTAAGCAGCAAACGGGACCGACGCCTCAGCGATTCCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGc                                                                                                                                                                                        <  1:104525/228‑1 (MQ=255)
                                                       ggaaaaGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCGGGCCGGGCTGGAGGACCATTTTGCCGATGAGGTAAATTGGGATGCCGTGGCGGATAAA‑TTTTCTCTTTCACTGCGTACGCTACATCGGCAGCTTAAGCAGCAAACGGGACTGACGCCTCAGCGATACCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGc                                                                                                                                                                                        <  2:1113015/236‑1 (MQ=255)
                                                       gTAAAAGCAGTTTGCGGGAGACCCTGAAAAACAGCGCACCCCGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGAGGAGGTGAATGGGGATGCCGTGGCGGAGCAA‑TTTTCTCTTTCCCTGCGTACGCTACACCGGCAGCTTAAGCAGCAAACGGGACTGACGCCTCAGCGATACCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGc                                                                                                                                                                                        <  1:363237/238‑1 (MQ=255)
                                                         aaaaGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGGTGGGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTTCTCTTTCACTTCGTTCGCTACACCGGCAGCTTAAGCAGAAAACGGGACTGACGCCTCCGCGATCCCTGAGCCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCa                                                                                                                                                                                      <  1:764001/238‑1 (MQ=255)
                                                                gTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTTCTCTTTCCCTGCGTACGCTACCTCGGCAACTTTAGCAGCAAACCGGACTGACGCCTCAGCGTTATCTGAACCCCCCGCGACCGATGAAAGCCCCGCCTCTGCTACGCCCCAGCACGGCAAGCGTTaat                                                                                                                                                                              >  1:487619/1‑237 (MQ=255)
                                                                gTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTTCTCTTTCACTGCGTACGCTACATCGGCAGCTTAAGCAGCAAACGGGACTGACGCCTCAGCGATACCTGAACCGCCTTCGACTGATGAAAGCCCGACATCTGCTCCGCCACAGCGAGGCCAGCgct                                                                                                                                                                                 >  2:750224/1‑234 (MQ=255)
                                                                gTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTTCTCTTTCACTGCGGACGCTACATCGGCAGCTTAAGCAGCAAACGGGACTGACGCCTCAGCGATACCTGAACCGCCTGCGACTGATGAAAGGCCGACCTCTGCTACGCCACCACGAGGCCAGCGGTaat                                                                                                                                                                              >  1:116351/1‑237 (MQ=255)
                                                                   tGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTTCTCTTTCACTGCGTACGCTACATCGGCAGCTTAAGCAGCAAACGGGACTGACGCCTCAGCGATACCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGAc                                                                                                                                                                           >  2:264905/1‑239 (MQ=255)
                                                                    gcgggAGAACCTGGAAAACAGCGCATCCCGTCCCAGCTTGCTTGTGGCCTGGCTGTAGGACCATTTTGCCGAGGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTTCTCTTTCACTGCGTACGCTACATCGGCAGCTTAAGCAGCAAACGGGACTGACGCCTCAGCGATACCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACa                                                                                                                                                                          <  1:322560/236‑1 (MQ=255)
                                                                    gaaGGAGAACCGGGAAAACAGCGCATCACGTTCCAACTTGCTTTGGGCTGGGCTGGAGGCCCATTTTGCGGAGGGGGGAAATGGGGAGGCCGGGGGGGATCAA‑TTTTCTCTTTCCCTGCGTACGCTACATCGGCAGCTTAAGCAGCAAACGGGACTGACGCCCAAGCGATCCCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACa                                                                                                                                                                          <  1:6111/237‑1 (MQ=255)
                                                                     gaggaaacccggtaaaaCACCGCATCACGTCTCAACTTGCTTCGGGCCGGGCGGGAGGACCATTTTCCCGATGAGGTGAATGGGGATGTCGTGGCGGATCAA‑TTTTCTCTTTCACTGCGTACGCTACATCGGCAGCTTAAGCAGCAAACGGGACTGACGCCTCAGCGATCCCGGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACa                                                                                                                                                                          <  1:1778/225‑1 (MQ=255)
                                                                         agaACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTTCTCTTTCACTGCGTACGCTACATCGGCAGCTTAAGCAGCAAACGGGACTGACGCCTCAGCGATACCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCg                                                                                                                                                                       >  2:306849/1‑237 (MQ=255)
                                                                          gacctaggAAAACAGCGCTTCATGTCTCATCTTGCTTCCGCCTTGGTTGGATGTCTATTCTGCCGATGGGGTGAATGGGGATTCCTTGGC‑GATAAACTTTTCTCTTTCCCTGTGTAAGGCACAGCGCCGTCTTAAGTAGCAAGCGGGGCTGACGCCCCGGCAATCCCTCAACCGCATGCGACTGATGAAAGCCCGCCATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGcc                                                                                                                                                                     <  1:90707/232‑1 (MQ=255)
                                                                          gaACCTGGAAAAAAGGTCACCGCGTCTCAACTTGCTTCTGGCGGGGCTGGAGGACCTTTTTGCCGATGAGGTGAATTGGGATGCCTGGGCGGATCAA‑TTTTCTCTTCCCCTGCGTACGCTACATCGGCAGCTTAAGCAGCAAAGGGGACTGACGCCTCAGCGATACCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGcc                                                                                                                                                                     <  1:987917/238‑1 (MQ=255)
                                                                            tgagggAAATCAGCACATCACGTCCCAGTTTGCGTCTTGGATGGCGGGACGGATATTTTGCCGAAGAGCTAAATTGGCATGCTGGGGGGGATCAA‑TTTTCTCTTCCACCGGGTCGGCTACAGCGGGAGTTTAAGGACCAAACGGGCCTCGCCCCTCAGAAATCCGGTACCCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGAGGCAGCGAGGCCAGCGTTACTGACTTCGCCTa                                                                                                                                                                   <  1:637293/234‑1 (MQ=255)
                                                                             ccTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTTCTCTTTCACTGCGTACGCTACATCGGCAGCTTAAGCAGCAAACGGGACTGACGCCTCAGCGATACCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCCCAGCGAGGCCAGCGTTACt                                                                                                                                                                              >  2:104525/1‑226 (MQ=255)
                                                                                  aaaaCAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTTCTCTTTCACTGCGTACGCTACATCGGCAGCTTAAGCAGCAAACGGGACTGACGCCTCAGCGATACCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGCTACTGACACCGCCTACCGCtgt                                                                                                                                                            >  2:960063/1‑239 (MQ=255)
                                                                                       caccccacccGTCACAACTGGCGTCATGCCTGGCGTGAGCACCATTTTGCCGAGGAGGTAACTGGGGATGCCTTGGCGGATCAA‑TTTTCTCTTTCACTGCGTCCGCTCCCCCGGCACCTAAACCACCAAACGGGACTGACGCCCCAGCGATCCCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGa                                                                                                                                                         <  1:306849/228‑1 (MQ=255)
                                                                                       aGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTTCTCTTTCACTGCGTACGCTACATCGGCAGCTTAAGCAGCAAACGGGACTGACGCCTCAGCGATACCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGAt                                                                                                                                                        >  2:1041122/1‑238 (MQ=255)
                                                                                        gcgcATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTTCTCTTTCACTGCGTACGCTACATCGGCAGCTTAAGCAGCAAACGGGACTGACGCCTCAGCGATACCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGCTTc                                                                                                                                                      >  2:1023275/1‑239 (MQ=255)
                                                                                        gcgcATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTTCTCTTTCACTGCGTACGCTACATCGGCAGCTTAAGCAGCAAACGGGACTGACGCCTCAGCGATACCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTc                                                                                                                                                      >  1:767006/1‑239 (MQ=255)
                                                                                         cgcATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTTCTCTTTCACTGCGTACGCTACATCGGCAGCTTAAGCAGCAAACGGGACCGACGCCTCAGCGATACCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCa                                                                                                                                                     >  2:1186916/1‑239 (MQ=255)
                                                                                           cATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTTCTCTTTCACTGCGTACGCTACATCGGCAGCTTAAGCAGCAAACGGGACTGACGCCTCAGCGATACCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTTTGGa                                                                                                                                                         >  2:637293/1‑233 (MQ=255)
                                                                                           cATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTTCTCTTTCACTGCGTACGCTACATCGGCAGCTTAAGCAGCAAACGGGACTGACGCCTCAGCGATACCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGc                                                                                                                                                   >  2:413158/1‑239 (MQ=255)
                                                                                                    tcAACTTGCTTCGGGCCTGGCTGGAGGACCATTTGGCCGATGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTTCTCTTTCACTGCGTACGCTACACCGGCAGCTTAAGCACCAAACGGGACTGACGCCTCAGCGATCCCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAAc                                                                                                                                          <  2:585977/239‑1 (MQ=255)
                                                                                                              tCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTTCTCTTTCACTGCGTACGCTACATCGGCAGCTTAAGCAGCAAACGGGACTGACGCCTCAGCGATACCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGa                                                                                                                                <  2:767574/239‑1 (MQ=255)
                                                                                                                       gctggAGGACCATTTTGCCGATGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTTCTCTTTCACTGCGTACGCTACATCGGCAGCTTAAGCAGCAAACGGGACTGACGCCTCAGCGATACCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTc                                                                                                                       >  2:429129/1‑239 (MQ=255)
                                                                                                                            aggaCCATTTTGCCGATGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTTCTCTTTCACTGCGTCCGCTACATCGGCAGCTTAAGCAGCAAACGGGACTGACGCCTCAGCGATCCCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAAc                                                                                                                                          <  2:648634/215‑1 (MQ=255)
                                                                                                                            aggaCCATTTTGCCGATGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTTCTCTTTCACTGCGTACGCTACATCGGCAGCTTAAGCAGCAAACGGGACTGACGCCTCAGCGATACCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAAc                                                                                                                                          >  1:648634/1‑215 (MQ=255)
                                                                                                                               aCCATTTTGCCGATGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTTCTCTTTCCCTGCGTCCGCTACATCGGCGGTTTAAGCAGCAACCGGGCCTGACGCCTCAGCGATACCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCgag                                                                                                                 <  2:1186349/237‑1 (MQ=255)
                                                                                                                                ccATTTTGCGGAGGAGGTGAATTGGAATGCGGTGGCGGATCAA‑TTTTCTCTTTCACTGCGTACGCTACATCGGCAGCTTAAGCAGCAAACGGGACTGACGCCTCAGCGATCCCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGagag                                                                                                               <  2:1148969/238‑1 (MQ=255)
                                                                                                                                 cATTTTAGCGATGAGGTGAAGTGGGAGGCCGTGGGGGATAAA‑TTTCCTCTTTCACGGCGTACGTTACAGCGGCACCTAAAGAAGCAAACGGGAGTGAGGCCACAGCGATGCCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGtt                                                                                                             <  1:1091956/239‑1 (MQ=255)
                                                                                                                                 actttggcctgtggggTGAGTGGGGAGCCAGTGGCGGAATAT‑TTCTCTCTTCCCCTGTGTACGCTACATGGGCAGCTTAAGCAACAAACGGGGATGCCGCTCCGGCCATCCATGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGtt                                                                                                             <  1:1186916/225‑1 (MQ=255)
                                                                                                                                         cGATGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTTCTCTTTCACTGCGTACGCTACATCGGCAGCTTAAGCAGCAAACGGGACTGACGCCTCAGCGATACCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGt                                                                                                     <  2:767006/239‑1 (MQ=255)
                                                                                                                                         cGATGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTTCTCTTCCACTGCGTACGCTACATCGGCAGCTTAAGCAGCAAACGGGACTGACGCCTCAGCGATACCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGt                                                                                                     <  2:11405/239‑1 (MQ=255)
                                                                                                                                            tGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTTCTCTTTCACTGCGTACGCTACATCGGCAGCTTAAGCAGCAAACGGGACTGACGCCTCAGCGATACCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCAc                                                                                                  <  2:115989/239‑1 (MQ=255)
                                                                                                                                                        ggATGCCGTGGCGGATCAA‑TTTTCTCTTTCACTGCGTACGCTACATCGGCAGCTTAAGCAGCAAACGGGACTGACGCCTCAGCGATACCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTc                                                                                      >  2:708169/1‑239 (MQ=255)
                                                                                                                                                            gCCGTGGCGGATCTA‑TTTGGTCTCTCATGGGGCGCACGACATCGGCGCGTGAACGAGGAAACGGGTGGGAGGCCTACGCTAGACCTGAACCGCCTGCGAGTGATGAAAGCTCGACATCTGATACGCCTCAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGca                                                                                   <  1:20123/238‑2 (MQ=255)
                                                                                                                                                             ccGTGGCGGATCAA‑TTTTCTCTTTCACTGCGTACGCTACATCGGCAGCTTAAGCAGCAAACGGGACTGACGCCTCAGCGATACCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACCGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAg                                                                                 >  1:5800/1‑239 (MQ=255)
                                                                                                                                                             ccGTGGCGGATCAA‑TTTTCTCTTTCACTGCGTACGCTACATCGGCAGCTTAAGCAGCAAACGGGACTGACGCCTCAGCGATACCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAACGGTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGGAACAAATTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGCTATTCGcccg                                                                                 >  1:251531/1‑237 (MQ=255)
                                                                                                                                                                            tgtataagcgacaggCGTACGCTACACCGGCAGCTTAAGCAGCAAACGGGCCTGACGCCTCAGCGATCCCTAAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCt                                                                    <  1:775739/224‑1 (MQ=255)
                                                                                                                                                                                 tctTTCACTGCGTACGCTACATCGGCAGCTTAAGCAGCAAACGGGACTGACGCCTCAGCGATACCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGc                                                              >  2:600367/1‑239 (MQ=255)
                                                                                                                                                                                  ctTTCACTGCGTACGCTACATCGCCAGCTTAGGCAGCAAACGGGACTGACGCCTCAGCGATACCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCa                                                             <  2:87984/239‑1 (MQ=255)
                                                                                                                                                                                        cTGCGTACGCTACTTCGGCAGCTAAAGCAGCAAGCGGGCCTGACGCCTCACCGATCCCTAAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAAcg                                                       <  2:487619/239‑1 (MQ=255)
                                                                                                                                                                                        cTGCGTACGCTACATCGGCAGCTTAAGCAGCAAACGGGACTGACGCCTCAGCGATACCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAAcg                                                       <  2:122216/239‑1 (MQ=255)
                                                                                                                                                                                             tACGCTACATCGGCAGCTTAAGCAGCAAACGGGACTGACGCCTCAGCGATACCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACcgcg                                                                                              >  1:990504/1‑195 (MQ=255)
                                                                                                                                                                                             tACGCTACATCGGCAGCTTAAGCAGCAAACGGGACTGACGCCTCAGCGATACCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACcgcg                                                                                              <  2:990504/195‑1 (MQ=255)
                                                                                                                                                                                             tACGCTACATCGGCAGCTTAAGCAGCAAACGGGACTGACGCCTCAGCGATACCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGaa                                                   >  2:757301/1‑238 (MQ=255)
                                                                                                                                                                                                  accacgggaaGCTAAAGCAGCAACCGGGACTGCCCCCCCAGCGATACCGAAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAAtcttct                                             <  1:865618/230‑1 (MQ=255)
                                                                                                                                                                                                     cgcGGCAGCTTAAGCAGCAAACGGGACTGACGCTCCAGCGATCCTTAAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCaa                                          <  2:617656/237‑1 (MQ=255)
                                                                                                                                                                                                     atggccAGCTTAAGCAGCAACAGGGACTGACGCCCGAGCGATACCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCaa                                          <  1:863482/234‑1 (MQ=255)
                                                                                                                                                                                                     aTCGGCAGCTTAAGCAGCAAACGGGACTGACGCCTCAGCGATACCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATa                                                          >  2:775739/1‑223 (MQ=255)
                                                                                                                                                                                                     aTCGGCAGCTTAAGCAGCAAACGGGACTGACGCCTCAGCGATACCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCa                                           >  1:742379/1‑238 (MQ=255)
                                                                                                                                                                                                     aTCGGCAGCTTAAGCAGCAAACGGGACTGACGCCTCAGCGATACCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCa                                           >  1:89881/1‑238 (MQ=255)
                                                                                                                                                                                                     aTCGGCAGCTTAAGCAGCAAACGGGACTGACGCCTCAGCGATACCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCa                                           >  2:865618/1‑238 (MQ=255)
                                                                                                                                                                                                     aTCGGCAGCTTAAGCAGCAAACGGGACTGACGCCTCAGCGATACCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCa                                           >  2:198145/1‑238 (MQ=255)
                                                                                                                                                                                                       cGGCAGCTTAAGCAGCAAACGGGACTGACGCCTTAGCGATACCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAggg                                                                               >  1:99633/1‑200 (MQ=255)
                                                                                                                                                                                                       cGGCAGCTTAAGCAGCAAACGGGACTGACGCCTCAGCGATACCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACg                                        >  2:705092/1‑239 (MQ=255)
                                                                                                                                                                                                       cGGCAGCTTAAGCAGCAAACGGGACTGACGCCTCAGCGATACCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGCGTTTCTGCAATAACGAGAATCTTCTCAACg                                        >  1:695868/1‑239 (MQ=255)
                                                                                                                                                                                                          cAGCTTAAGCAGCAAACGGGACTGACGCCTCAGCGATACCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTAt                                     >  2:506394/1‑239 (MQ=255)
                                                                                                                                                                                                          cAGCTTAAGCAGCAAACGGGACTGACGCCTCAGCGATACCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTAt                                     >  2:149119/1‑239 (MQ=255)
                                                                                                                                                                                                              ttAAGCAGCAAACGGGACTGACGCCTCAGCGATCCCTGCACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGACCCAGCGACCCCCGCCTTCCTGACATCGCCTATCGCTGCGGCTTCAGCGACAGTAACCACTTTTCTACGCTTTTTCGCCGCGAGTTTACCTGGTCACCCCGTGATATTCGCCAGGGCCGCGCTAGCTTTCTGCAATCACGCGAATCTTCACACCGTATTTGt                                 >  1:219159/1‑239 (MQ=255)
                                                                                                                                                                                                              ttAAGCAGCAAACGGGACTGACGCCTCAGCGATACCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTCCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGAGTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCCCACCGTGATATTCGCCAGGGCCGGGATGGTTTTTTTCCATAACGCTAATCTTTCCAACGCATgtgt                                 >  1:171972/1‑239 (MQ=255)
                                                                                                                                                                                                              tgaaGCAACAACCGGGCCGGCCCCCTCGGCGATACCTAACCCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTAttt                                   <  1:1078146/235‑1 (MQ=255)
                                                                                                                                                                                                              tacagcagcAAACTGTACTGACCCCTCCGCGAGCCCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCTAGGCCAGCGTTACTGACATCGCCTATCGCTTTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGt                                 <  1:1041122/236‑1 (MQ=255)
                                                                                                                                                                                                                 agcagcAAGCGGGACTGTGTTCTCGGCTAGCCCGAAACCGCCTGCGGCGGATTAAAGCCCGGCATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTGTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACg                              <  1:919545/239‑1 (MQ=255)
                                                                                                                                                                                                                    agcaAACGGGACTGACGCCTCAGCGATCCCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACGcc                            <  2:749148/238‑1 (MQ=255)
                                                                                                                                                                                                                    accaAAGGGGCCGGACGCCTCAGCGATACCGGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACGcc                            <  1:708169/236‑1 (MQ=255)
                                                                                                                                                                                                                       aaaCGGGACTGACGCCTCAGCGATACCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACt                                                                                                        >  2:332541/1‑159 (MQ=255)
                                                                                                                                                                                                                       aaaCGGGACTGACGCCTCAGCGATACCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGt                                 <  2:219256/230‑1 (MQ=255)
                                                                                                                                                                                                                       aaaCGGGACTGACGCCTCAGCGATACCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACGCCatat                        >  2:584481/1‑239 (MQ=255)
                                                                                                                                                                                                                        accGGGCCTGACGCCTCACCGATCCCTAAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCaa                                          <  2:312730/218‑1 (MQ=255)
                                                                                                                                                                                                                        aaCGGGACTGACGCCTCAGCGATACCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACGCCATAtt                       >  1:845261/1‑239 (MQ=255)
                                                                                                                                                                                                                          cGGGCCTGCCCCCCCAGCGATCCCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACGCCATATTGc                     <  1:511200/239‑1 (MQ=255)
                                                                                                                                                                                                                                 gACGCCGCAGCGATCCCTGACCCGCCTGCGACTGATGAAAGCCCGACAGCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACGCCATATTGCGaataat              <  2:1135138/239‑1 (MQ=255)
                                                                                                                                                                                                                                  ccgcctaagcatcaCCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTTATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACGCTATATTGCGaataat              <  2:326855/225‑1 (MQ=255)
                                                                                                                                                                                                                                  aCGCCTCAGCGATCCCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGCGTTTAACTGGTCACCGCGTGTTATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATATTCTCCACGTATTTGTaactgtctcttttacacat              >  1:312730/1‑220 (MQ=255)
                                                                                                                                                                                                                                  aCGCCTCAGCGATACCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACGCCATATTGCGAATAATc             >  2:690019/1‑239 (MQ=255)
                                                                                                                                                                                                                                     ccTCAGCGATACCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGCGTGTTTAACTGGTCACCGCGTGATATTCGCCAGCGACGGGATGGATTTCTGCACTAACGCGAATCTTCTCATCGTATTTGTACg                              >  1:572296/1‑219 (MQ=255)
                                                                                                                                                                                                                                       tCAGCGATACCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACGCCATAtt                       >  1:947288/1‑224 (MQ=255)
                                                                                                                                                                                                                                       tCAGCGATACCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACGCCATAtt                       <  2:947288/224‑1 (MQ=255)
                                                                                                                                                                                                                                       tCAGCGATACCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACGCCATATTGCGAATAATCAACt         >  1:722282/1‑238 (MQ=255)
                                                                                                                                                                                                                                       tCAGCGATACCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACGCCATATTGCGAATAATCAACt         >  1:1094273/1‑238 (MQ=255)
                                                                                                                                                                                                                                       tCAGCGATACCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACGCCATATTGCGAATAATCAACt         >  2:344562/1‑238 (MQ=255)
                                                                                                                                                                                                                                              tACCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACGCCATATTGCGAATAATCAACTTCGTtct  <  1:432468/238‑1 (MQ=255)
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                            
TTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGAATTGGGATGCCGTGGCGGATCAA‑TTTTCTCTTTCACTGCGTACGCTACATCGGCAGCTTAAGCAGCAAACGGGACTGACGCCTCAGCGATACCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACGCCATATTGCGAATAATCAACTTCGTTCT  >  NC_000913/4098152‑4098626

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A