Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F9 I0 R1	142	103.7	2603848	81.8%	2129947	227.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,665,631	G→A	10.4%	S60L (TCA→TTA)	gadX ←	acid resistance regulon transcriptional activator, autoactivator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,665,631	0	G	A	10.4%	373.2 / 5.5	131	S60L (TCA→TTA)	gadX	acid resistance regulon transcriptional activator, autoactivator
Reads supporting (aligned to +/- strand): ref base G (73/41); new base A (2/13); total (76/55)
Fisher's exact test for biased strand distribution p-value = 3.75e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 131 total aligned reads displayed.

GTTCAGAAGCAGCGGTATAAAGTGCTCATCCTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAG‑CATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGG‑AATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGTGCATGAAGATTTAAT‑GCCTCCTCCTTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑AACAC‑ACTTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAG‑TGATTGCATAGTTGACTTAATA‑TTACATAAACATATTACTGTTTATTAATGTAGCACGCCGC  >  NC_000913/3665399‑3665862

gTTCAGAAGCAGCGGTATAAAGTGCTCATCCTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAG‑CATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGG‑AATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGTGCATGAAGATTTAAT‑GCCTCCTCCTTGAGCATCGGGAGAAAT‑AATGAAA‑GCg                                                                                                                                                                                                                                           <  2:583610/239‑1 (MQ=255)

 ttCAGAAGCCGCGGTATAAAGTGCTCATCCTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAG‑CATTTCTGCATAGCGAAGATTCTTAGTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGG‑AATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGTGCATGAAGATTTAAT‑GCCTCCTCCTTGAGCATCGGGAGAAAT‑AATGAAA‑GCgt                                                                                                                                                                                                                                          <  2:842409/239‑1 (MQ=255)

 ttCAGAAGCAGCGGTATAAAGTGCTCATCCTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAG‑CATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGG‑AATATCTCTACTACAGTGATGAACGCGTAATGAAGAAACTTTTTT‑A‑TGTGCATGAAGATTTAAG‑GCCTCCTCCTTGAGAATCGGGAGAAAT‑ATTGAAA‑GCgt                                                                                                                                                                                                                                          >  1:357177/1‑239 (MQ=255)

          agcGGTATAAAGTGCTCATCCTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAG‑CATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGG‑AATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGTGCATGAAGATTTAAT‑GCCTCCTCCTTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCtt                                                                                                                                                                                                                                   <  2:72870/237‑1 (MQ=255)

              gtaataaGTTCTCAACCTCAAGAAAAACAGATAACAACGCAAAGATTAATTCTCTTTTACGTAG‑GATTTCTGCATAGCGAAGATTCTTATGCTGCGATAGTTGCGCAACTTCCTGAAAAACAGG‑AATAGCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGTGCATGAAGATTTAAT‑GCCTCCTCCTTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGac‑ac                                                                                                                                                                                                                             <  2:531993/233‑1 (MQ=255)

                      ttcacATCCTCAAGAAAAACAGATAACAACGGAAAGATTAATTCTCTTTTACGTAG‑CATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGG‑AATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGTGCATGAAGATTTAAT‑GCCTCCTCCTTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACaaa                                                                                                                                                                                                                    <  1:1245409/235‑1 (MQ=255)

                      tGCTCATCCTCAAGAAAAACAGATAACAACGCCAAGATTAATGCTCTTTTACGTAG‑CATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGG‑AATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGTGCATGAAGATTTAAT‑GCCTCCTCCTTTAGCATCGGGAGAAAT‑AATGAAA‑GCGGGTCCCTTTCC‑ACGAAAACaaa                                                                                                                                                                                                                    >  1:1255281/1‑239 (MQ=255)

                      tGCTCATCCTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAG‑CATTTCTGCATAGCGAAGATTCTTATTCTGCGATATTTGCGCAACTTCCTGAAAAACAGG‑AATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTAT‑A‑TGTGCATGAAGATTTAAT‑GCCTCATCCTTGAGCTTCTGGAGAAAT‑AATGAAA‑GAGGGTCCCTTGAC‑ACAaaaaaaaa                                                                                                                                                                                                                    >  1:1040359/1‑239 (MQ=255)

                        cTCATCCTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAG‑CATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGG‑AATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGTGCATGAAGATTTAAT‑GCCTCCTCCTTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAAt                                                                                                                                                                                                                  >  2:534787/1‑239 (MQ=255)

                        cTCATCCTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAG‑CATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGG‑AATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGTGCATGAAGATTTAAT‑GCCTCCTCCTTGAGCATCGGGAGAAAT‑AATGAAA‑GCGGGTCCCTTGAC‑ACGAATACAAAAt                                                                                                                                                                                                                  >  2:1256413/1‑239 (MQ=255)

                        cTCATCCTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAG‑CATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGG‑AATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGTGAATGAAGATTTAAT‑GCCTCCTCCTTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAAAAAAAAAt                                                                                                                                                                                                                  >  1:1136680/1‑239 (MQ=255)

                         tCATCCTCAAGAAAAACAGATAACACCGCAAAGATTAATGCTCTTTTACGTAG‑CATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGG‑AATATCTCTACTACAGTGATGAACGAGAAATGAAGAAACTTTTTT‑A‑TGTGCATGAAGATTTAAT‑GCCTCCTCCTTGGGCATAGGGAGAAAT‑AATAAAA‑GGGTGTCCCTTGAC‑ACGAaaaaaaaaat                                                                                                                                                                                                                 >  1:959030/1‑237 (MQ=255)

                         tCATCCTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAG‑CATTTCTGCATAGCGTAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGG‑AATATCTCTACTTCTGTGATGAACGAGTAATGAAGAAACTTTTTG‑A‑TGTGCATGAAGATTTACA‑TCCTCCTCCTTGTGCATCGAGAGAAAT‑AATGCAA‑GCGTGTACCTTGaaaaacaaaaaaaaat                                                                                                                                                                                                                  >  1:556728/1‑224 (MQ=255)

                         tCATCCTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAG‑CATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGG‑AATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGTGCATGAAGATTTAAT‑GCCTCCTCCTTGAGCATCGGGGGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAAtt                                                                                                                                                                                                                 >  2:532828/1‑239 (MQ=255)

                         tCATCCTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAG‑CATTTCTGCATAGCGAAGATTATTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGG‑AATATCTCTACTACAGTGTAGAACGAGTAATGAAGACACTTTTTA‑C‑TTTGCATGAAGATTGAAT‑GCCTCCTACTTGTGCATCGGTTGAAAT‑AATGAAA‑ACGGGTCCCATAac‑agagaaaaaaaaat                                                                                                                                                                                                                 >  1:360326/1‑226 (MQ=255)

                           aTCCTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAG‑CATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGG‑AATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TTTGCATGAAGTTTTAAT‑GCATCCTCCTTACGCATCGGGCGAAAT‑AATTAAA‑GATAGTCCCTTGCA‑CCAAAAACAAAAtttt                                                                                                                                                                                                               >  1:529693/1‑239 (MQ=255)

                           aTCCTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAG‑CATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGG‑AATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGTGCATGAAGATTTAAT‑GCCTCCTCCTTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAAtttt                                                                                                                                                                                                               <  2:1297100/239‑1 (MQ=255)

                             ccTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAG‑CATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGG‑AATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGTGCATGAAGATTTAAT‑GCCTCCTCCTTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAAAAAAAAAttttaa                                                                                                                                                                                                             >  1:655987/1‑237 (MQ=255)

                             ccTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCGTTTACGTAG‑CATTGCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGG‑AATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGTGCATGAAGATTTAAT‑GCCTCCTCCTTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑a                                                                                                                                                                                                           <  2:556728/239‑1 (MQ=255)

                             ccTCAAAGAAAGAAGATAAAAACGCAATGATTAGTCCTCTTTTACGTTGTCATTT‑TGCATAAGGAATATTCTTATTCCGCGCTAGTTGGTCAACTTCCTGAAAAACAGG‑AATATCGCTACTGCAGTGATGGACGAGTAATGAAGAAACTTTTTT‑A‑TTTGCATGATGATCTAAT‑GCCTCCTCCTTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGAGCCTTGAG‑ACGAATGCAGAGTTTT‑C‑a                                                                                                                                                                                                           <  1:468220/239‑1 (MQ=255)

                              cttcaGAAAACCATATTACAACGCTAAGATTAATGCTCTTTTACGTAG‑CATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAATTTCCTGAAAAACAGG‑AATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGTGCATGAAGATTTAAT‑GCCTCCTCCTTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑aa                                                                                                                                                                                                          <  1:75719/235‑1 (MQ=255)

                              cTCAAGAAAAACTGATAACAACGGAAAGATTTATGCTCTTTTACGTAG‑CATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGG‑AATATCTCTACTACAGTTATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGTGCATGAAGATTTAAT‑GCCTCCTCCTTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑aa                                                                                                                                                                                                          <  1:534787/239‑1 (MQ=255)

                              cTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAG‑CATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGG‑AATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGTGCATGAAGATTTAAT‑GCCTCCTCCTTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑aa                                                                                                                                                                                                          <  2:529693/239‑1 (MQ=255)

                              cTCAAGAAAAACAGATAACAACGAAAAGATTAATGCTCTTTTACGTAG‑CATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGG‑AATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGTGCATGAAGATTTAAT‑GCCTCCTCCTTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑aa                                                                                                                                                                                                          <  2:655987/239‑1 (MQ=255)

                                ccaaaaaaacaaaatataactgaaaGTTTAATTGTTTTTTACCTTG‑CATTTCCTGATAAGGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGG‑AATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGTGCATGAAGATTTAAT‑GCCTCCTCCTTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑Acca                                                                                                                                                                                                        <  1:336015/217‑1 (MQ=255)

                                cAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAG‑CATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGG‑AATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGTGCATGAAGATTTAAT‑GCCTCCTCCTTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑Aaca                                                                                                                                                                                                        <  2:546056/239‑1 (MQ=255)

                                 aaGAAAAACGAAAAACAAATAAAATATTAATGCGTTTTTACGTAG‑CATTTCTGCATAGCAAAGATTCTTATTCTGTGATAGGTGCGCAACTGCCTGAAAAACAAG‑AATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGTGCATGAAGATTTAAT‑TCCTCCTCCTTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTTTCCCTTGAC‑ACGAATACAAAATGTT‑c‑accac                                                                                                                                                                                                       <  1:1024595/239‑1 (MQ=255)

                                  aGAAAAACAAATAACAACGCAAAATTTAATGCTCTTTTACGTAG‑CATTTCTGCATAGCGAAGATGCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGG‑AATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGTGCATGAAGATTTAAT‑GCCTCCTCCTTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑Aacac‑a                                                                                                                                                                                                     <  1:94251/239‑1 (MQ=255)

                                     aaaaCAGATAACAACGCAAAGATTAATGCTCTTTTACGTAG‑CATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGG‑AATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGTGCATGAAGATTTAAT‑GCCTCCTCCTTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑Aacac‑ac                                                                                                                                                                                                    <  2:61344/237‑1 (MQ=255)

                                       aaCAGATAACAACGCAAAGATTAATGCTCTTTTACGTAG‑CATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGG‑AATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGTGCATGAAGATTTAAT‑GCCTCCTCCTTGAGCATCGGGGGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑AAAAC‑ACTTAt                                                                                                                                                                                                >  2:40620/1‑239 (MQ=255)

                                                  acccAAAGATTAATTCTCTTTTTCGTGA‑CCTTTTTGCAAAGCGAAGATTCTTATTCTGCAATAGTTGCGCAACTTCCTGAAAAACAGG‑AATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGTGCATGAAGATTTAAT‑GCCTCCTCCTTGAGCACCGGGAGAAAT‑AATGAAA‑GCGTGTTCCTTGAC‑ACGAATACAAAATTTT‑C‑AACAC‑ACTTATCTACTCGAAtt                                                                                                                                                                                     <  1:507493/236‑1 (MQ=255)

                                                                    ttttACGTAG‑CATTTCTGCATAGCGAAGATTCTTATTCTGCGCTAGTTGCGCAAATTCCTGAAAAACAGG‑AATATCTCTACTACAGTGATGAACGAGTCATGAAGAAACTTTTTT‑A‑TGTGCATGAAGATTTAAT‑GCCTCCTCCTTGAGCCTCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑AACAC‑ACTTATCTACTCGAATTTGGC‑TTGCCTCCGCaaaa                                                                                                                                                                  >  2:1070255/1‑239 (MQ=255)

                                                                    ttttACGTAG‑CATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGG‑AATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGTGCATGAAGATTTAAT‑GCCTCCTCCTTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑AACAC‑ACTTATCTACTCGAATTTGGC‑TTGCATCCGCaaaa                                                                                                                                                                  >  2:1174453/1‑239 (MQ=255)

                                                                    ttttACGTAG‑CATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGG‑AATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGTGCATGAAGATTTAAT‑GCCTCCTCCTTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑AACAC‑ACTTATCTACTCGAATTTGGC‑TTGCATCCGCaaaa                                                                                                                                                                  >  2:94527/1‑239 (MQ=255)

                                                                    ttttACGTAG‑CATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGG‑AATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGTGCATGAAGATTTAAT‑GCCTCCTCCTTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑AACAC‑ACTTATCTACTCGAATTTGGC‑TTGCATCCGCaaaa                                                                                                                                                                  >  2:974613/1‑239 (MQ=255)

                                                                    ttttACGTAG‑CATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGG‑AATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGTGCATGAAGATTTAAT‑GCCTCCTCCTTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑AACAC‑ACTTATCTACTCGAATTTGGC‑TTGACTCCCCaaaa                                                                                                                                                                  >  1:18165/1‑239 (MQ=255)

                                                                    ttttACGAAG‑CATATCTGCATAGCGAAGGTTCTTATTCTGCGATCGTTGCGCAACTTCCTGAAAAACAGG‑AATATCTCTACTAAAGTGATGAACGTGTAATGAAGCAAGTTTTTT‑A‑TGTGCATGATGATTTATT‑GCCTCCTCCTTGAGCATCGGTAGAAAT‑AATGAAA‑CCGCGTCCTTTGAT‑ATGTATCGAAAATTTC‑C‑AAAAC‑ACTTACCAACCCCAATTTTGC‑TTCAATACGCaaaa                                                                                                                                                                  >  1:239877/1‑239 (MQ=255)

                                                                       tACGTAG‑CATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGG‑AATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGTGCATGAAGATTTAAT‑GCCTCCTCCTTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑AACAC‑ACTTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAAcc                                                                                                                                                               >  2:134976/1‑239 (MQ=255)

                                                                               cATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGG‑AATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGTGCATGAAGATTTAAT‑GCCTCCTCCTTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGCATACAAAATTTT‑C‑AACAC‑ACTTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACC‑AGGTCa                                                                                                                                                        >  2:834577/1‑238 (MQ=255)

                                                                               cATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGG‑AATATCTCTACTACAGCGATGAACGAGGAATGAAGAAACTTTTTT‑A‑TGTGAATGAAGATTTAAT‑GCCTCCTCCTTGAGCATCGGGGGCAAT‑AATGACA‑GCGAGTCCCTTCAA‑ACGTATATAAATTTTT‑C‑AAAAC‑CATTCATAACTCGATTTTGCC‑TTGCATCCCCAAAAACA‑Agtaac                                                                                                                                                        >  1:722523/1‑234 (MQ=255)

                                                                                         tAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGG‑AATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGTGCATGAAGATTTAAT‑GCCTCCTCCTTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑Aacac‑a                                                                                                                                                                                                     >  2:328572/1‑185 (MQ=255)

                                                                                         tAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGG‑AATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGTGCATGAAGATTTAAT‑GCCTCCTCCTTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑Aacac‑a                                                                                                                                                                                                     <  1:328572/185‑1 (MQ=255)

                                                                                            cGAAGATTCTTATTCTGCGATATTTGCGCAACTTCCTGAAAAACAGG‑AATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGTGCATGAAGATTTAAT‑GCCTCCTCCTTGAGCATCGGGAGAAAT‑AATGAAA‑Gc                                                                                                                                                                                                                                            >  2:752949/1‑147 (MQ=255)

                                                                                            cGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGG‑AATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGTGCATGAAGATTTAAT‑GCCTCCTCCTTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑AACAC‑ACTTATCTACTCGAATTTGGC‑TTGCATCCGCAAAACCC‑AGGCCACCGCCATTAAAATa                                                                                                                                          >  2:1252337/1‑239 (MQ=255)

                                                                                                 aTTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGG‑AATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGTGCATGAAGATTTAAT‑GCCTCCTCCTTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCtt                                                                                                                                                                                                                                   <  2:726584/151‑1 (MQ=255)

                                                                                                      tATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGG‑AATATCTCTACTACAGTGATGAACGAGTAATGAGGAAACTTTTTT‑A‑TGTGCATGAAGATTTAAT‑GCCTCCTCCTTGAGCATCGGGAGAAAT‑AATGAAA‑GCGAGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑AACAC‑ACTTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACC‑CGGCCAACGCCATTAAAATAGCGaaaaaca                                                                                                                                >  1:498476/1‑233 (MQ=255)

                                                                                                           tGCGATAGTTGCGCAACTTCCTGAAAAACAGG‑AATATCTCTCCTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGTGCATGAAGATTTAAT‑GCCTCCTCCTTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑AACAC‑ACTTATCTACTCGAATTTGGC‑TTGAATCCGCACAAACC‑AGGTCACCGCCATTAAAATAGCGATCtttcccttt                                                                                                                           >  2:182347/1‑232 (MQ=255)

                                                                                                           tGCGATAGTTGCGCAACTTCCTGAAAAACAGG‑AATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGTGCATGAAGATTTAAT‑GCCTCCACCTTGATCATCGGGAGAAAT‑AATGAAA‑GCTTGTCCCTTGAC‑CCGATTa                                                                                                                                                                                                                        >  1:726584/1‑151 (MQ=255)

                                                                                                             cGATAGTTGCGCAACTTCCTGAAAAACAGG‑AATATCTCTACTCCAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGTGCATGAAGATTTAAT‑GCCTCCTCCTTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑ac                                                                                                                                                                                                          >  1:879100/1‑160 (MQ=255)

                                                                                                             cGATAGTTGCGCAACTTCCTGAAAAACAGG‑AATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGTGCATGAAGATTTAAT‑GCCTCCTCCTTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑AACAC‑ACTTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTa                                                                                                                          >  2:733632/1‑238 (MQ=255)

                                                                                                                   ttGCGCAACTTCCTGAAAAACAGG‑AATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGTGCATGAAGATTTAAT‑GCCTCCTCCTTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑AACAC‑ACTTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAAc                                                                                                                        >  2:365936/1‑234 (MQ=255)

                                                                                                                     gcgcAACTTCCTGAAAAACAGG‑AATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGTGCATGAAGATTTAAT‑GCCTCCTCCTTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑AACAC‑ACTTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACC‑AGGCCACCGCCATTAAAATAGCGAAATTAACCATAAACCAAggga                                                                                                                 >  1:1010255/1‑237 (MQ=255)

                                                                                                                     gcgcAACTTCCTGAAAAACAGG‑AATATCTCTACTACAGTGATGAAAGAGTAATGAAGAAACTTTTTT‑A‑TGTGCATGAAGATTTAAT‑GCCTCCTCCTTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑AACAC‑ACTTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACC‑AGGTCACCGCCATTAAAAAAGCACTATTAACCATTAACCATgggg                                                                                                                 >  2:641902/1‑237 (MQ=255)

                                                                                                                      cgcaattgtatGAAAAACGGG‑AATTTTCTTACTAAGTTTATGAACGAGTAATAAAGAAACTTTTTA‑A‑TGGCCAAAAAATTTTAAG‑CCCTCCACCTGGAGCAGCGGGAAAAAT‑AATGAAG‑GGGTGCCCCCAAAC‑ACAAATAAAAAATTTA‑A‑AACAC‑ATTTATCTACCCAATTTTGGC‑TTGAATCCGAAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTga                                                                                                                <  2:943548/229‑1 (MQ=255)

                                                                                                                         aatttccaaaaaaaCAAG‑AATACCTCTACTCAGGGGAAGAAAGAATAAGAAAAAAATTTTTTT‑A‑TGTAAAATAAGATTTAAT‑CCCCCCTCCTTGACCATGGGAAAAAAT‑AATAAAG‑GCGTGTCCCTTGAC‑ACGAATAAAAATTTTA‑C‑ACCCC‑ACTTATCTACTCGATTTTGGT‑TTGCACCCACAAAACCC‑GGTTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGaa                                                                                                             <  2:358150/230‑1 (MQ=255)

                                                                                                                          aatttcaaaaaaaaAGGAAATTTCCCTACGACATGGATAAACGAG‑AAAGAAAAAATTTTTTTGA‑GGTGAATGAAGATTAAGT‑CCCTCCCCCTGAAGCATCGGAAAAAAT‑AAGAAAA‑GCGTGCCCCTTGAC‑ACAAATACAAAATTTT‑C‑AACAC‑TCTTATCTACTCGAATTTGCC‑GTGCACCCGAAAAACCC‑AGATCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGaa                                                                                                             <  2:1255281/231‑1 (MQ=255)

                                                                                                                           tttccaaaaaaaCAGG‑AATATCTCTACTGAAGTAAGAAACGAGTAATGAAAAAATTTTTTT‑A‑TGGGAAGAAAGTTTTAAT‑GCCTCCTCCTTGGGCAGCGGGAGAAAA‑AATAAAG‑GGGTGTCCCTTGAC‑CCAAATA‑AAAATTTT‑CAAACCC‑TCTTATCTACCCAAATTTGGC‑TTGCACCCGAAAAAACC‑AGGCCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAata                                                                                                           <  2:1136680/232‑1 (MQ=255)

                                                                                                                               cTGAAAAACAGG‑AATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGTGCATGAAGATTTAAT‑GCCTCCTCCTTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑AACAC‑ACTTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATAtt                                                                                                       >  2:44077/1‑239 (MQ=255)

                                                                                                                                 aaaaaacaagatatattctcactacATTAATGAACGAGAAGTGAAGAATCTTTGTT‑G‑GGTGCATTAAGATCTACT‑CCCTCATCTTTGGCCAGAGTGAAAAAT‑AATGCAT‑GATTTCTCCTTGAA‑AGAAAAACAACTCTAT‑C‑ACCTC‑TCTTATCAACAAGAATTTGGT‑GTCCATCGGCAAAAATG‑AGTCCTCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATAttt                                                                                                      <  1:733632/219‑1 (MQ=255)

                                                                                                                                    aaaCAGG‑AATCTCTCTACTACAGTTAAGAAAGAAAAATAAAAAAATTTTTTT‑A‑GGTGCATGAAGATTAAAT‑CCCCCCCCCTTGAACATGGGGAAAAAT‑AATGAAA‑GCGTTCCCTTTGAC‑ACAAATAAAAAATTTC‑C‑AACAC‑ACTTATCTACTCAATTTTGGC‑TTGCACCCGCAAAACCC‑GGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGt                                                                                                  <  2:1010255/239‑1 (MQ=255)

                                                                                                                                          g‑AATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGTGCATGAAGATTTAAT‑GCCTCCTCCTTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑AACAC‑ACTTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCa                                                                                            >  2:236023/1‑239 (MQ=255)

                                                                                                                                             atatCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGTGCATGAAGATTTAAT‑GCCTCCTCCTTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATAAAAAATTTT‑A‑AACAC‑ACTTATCTACTCGAATTTGGC‑TTGAATCCGCAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTACACATGGTGAAAAAACATTTATGTCttcaaa                                                                                           >  1:1104902/1‑234 (MQ=255)

                                                                                                                                                  tctACTACAGGGATGAACGAGTAATAAAGAATCTTTTTA‑A‑TGTGAAAGAAATTTTAAC‑CCCTCCCCCTTGAGCATCGGAAAAAAT‑AATAAAA‑GCGTTCCCCTTGAC‑ACAAATACAAATTTTC‑C‑AACAC‑ATTTATCTACTCAATTTGGGC‑TTGCACCCAAAAAAACC‑AGGTCCCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGaa                                                                                                             <  2:665917/215‑1 (MQ=255)

                                                                                                                                                     actacAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGTGCATGAAGATTTAAT‑GCCTCCTCCTTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑AACAC‑ACTTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACC‑AGGTCACCGCCCTTAAAATAGCGATATTCACCATTAACCATGGTGAGAAAATATTTATGTCTTGCAAACGAAAtaa                                                                                  >  1:699988/1‑237 (MQ=255)

                                                                                                                                                         cAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGTGCATGAAGATTTAAT‑GCCTCCTCCTTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑aa                                                                                                                                                                                                          >  2:385069/1‑118 (MQ=255)

                                                                                                                                                         cAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGTGCATGAAGATTTAAT‑GCATCCTCCTTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑AACAC‑ACTTATCTACTCGAATTTGTC‑TTGCATCCGCAAAAACC‑AGGTCCCCGCCATTACAATCGCGATATTCACCATTAACCATGGTGAGAATATATATATGTCTGGCAAAGGCAAATAGACa                                                                              >  1:650116/1‑239 (MQ=255)

                                                                                                                                                          cgaggaagaagtaaaaTAAAGATAGTTTTTT‑T‑TGTGAATGAAGAATTAAC‑TCCTACGTGTTGATCCTGCGGAAAAAT‑AACAAAA‑GCGTTCCCCTTGAA‑CAGAAAAAAAATGTGG‑C‑AACAC‑CTTTTTTTGTGAGTATTGGGT‑TTGCCCCCAAAAAAACC‑AGGTACCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACaa                                                                             <  1:926960/225‑1 (MQ=255)

                                                                                                                                                           agtaatgaaagaaaaaaaaaaaaTTTTTTT‑AGTGGGAATAAATATTAAAT‑GCCTCCCCTTGGAGCAGGGGAAAAAAT‑AA‑GAAA‑GCGTGGCCCTTAAA‑ACAAAAAAAAAATTTTAC‑AACCT‑CTTTACCTATCAGATTTTGGC‑TGGCACCCGAAAAACCC‑AGGCCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACaa                                                                             <  2:1104902/219‑1 (MQ=255)

                                                                                                                                                            tgatgaACGAGTAATGAAGAAACTTTTTT‑A‑TGTGCATGAAGATTTAAT‑GCCTCCTCCTTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACaaa                                                                                                                                                                                                                    >  2:889179/1‑107 (MQ=255)

                                                                                                                                                            tgatgaACGAGTAATGAAGAAACTTTTTT‑A‑TGTGCATGAAGATTTAAT‑GCCTCCTCCTTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑a                                                                                                                                                                                                           <  1:519621/114‑1 (MQ=255)

                                                                                                                                                            tgatgaACGAGTAATGAAGAAACTTTTTT‑A‑TGTGCATGAAGATTTAAT‑GCCTCCTCCTTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑AACAC‑ACTTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACC‑AGGTACCCGCCATTAAAATAGCGATATTAACCATTAACCATGGGGAGAATATATTAATGTcattctcttaaacacatataac                                                                            >  1:665917/1‑217 (MQ=255)

                                                                                                                                                            taagcactgaaaaaTGAAAAACTTTTTTT‑A‑TGTGCATGAATTTTTAAG‑CCCCCCTCCTGGAACATGGGGAGAAAT‑AATAAAA‑GCTTGTCCTTTGAA‑ACAAATAAAAAATTTC‑C‑AACCC‑CTTTATCTACTCAATTTTGGC‑TTGCACCCAAAAAACCC‑AGGCCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACaa                                                                             <  2:945130/225‑1 (MQ=255)

                                                                                                                                                              atgaACGAGTAATGAAGAAACTTTTTT‑A‑TGTGCATGAAGATTTAAT‑GCCTCCTCCTTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑aa                                                                                                                                                                                                          >  1:886748/1‑113 (MQ=255)

                                                                                                                                                              atgaACGAGTAATGAAGAAACTTTTTT‑A‑TGTGCATGAAGATTTAAT‑GCCTCCTCCTTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑aa                                                                                                                                                                                                          <  2:886748/113‑1 (MQ=255)

                                                                                                                                                              atgaACGAGTAATGAAGAAACTTTTTT‑A‑TGTGCATGAAGATTTAAT‑GCCTCCTCCTTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑AACAC‑ACTTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACC‑AGGTCACCTCCATTAAAATAGCGCTATTCACCATGAACAATTGTGAGAATAAATTTATGTCTTGCAATCGCAATTAGTCAATTc                                                                          >  2:706516/1‑238 (MQ=255)

                                                                                                                                                              atgaACGAGTAATGAAGAAACTTTTTT‑A‑TGTGCATGAAGATTTAAT‑GCCTCCTCCTTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑AACAC‑ACTTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATg                                                                                                   >  2:410580/1‑213 (MQ=255)

                                                                                                                                                              atgaACGAGTAATGAAGAAACTTTTTT‑A‑TGTGCATGAAGATTTAAT‑GCCTCCTCCTTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑AACAC‑ACTTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGAcctgtct                                                                         >  2:859175/1‑233 (MQ=255)

                                                                                                                                                              atgaACGAGTAATGAAGAAACTTTTTT‑A‑TGTGCATGAAGATTTAAT‑GCCTCCTCCTTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑AACAC‑ACTTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTc                                                                          >  2:1110994/1‑238 (MQ=255)

                                                                                                                                                              atgaACGAGTAATGAAGAAACTTTTTT‑A‑TGTGCATGAAGATTTAAT‑GCCTCCTCCTTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑AACAC‑ACTTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACC‑AGGTCACCGCCATTAAAATAACATTATTCACCATTAACCATGGGGAGAAAATATTTATATATTGAATACGCAATTAAAAAATTcc                                                                         >  1:1226981/1‑239 (MQ=255)

                                                                                                                                                                 accgagaaaaaaaaaaaCTTTTTT‑A‑TGGGAATAAAATTTTAAT‑CCCTCCTCCTTGAGCATGGGGAAAAAA‑AAAGAAG‑GCGGGTCCTTTAAC‑AAAAATAAAAAATTTC‑C‑AACCC‑TCTTATCTACTAAATTTTGGA‑TTGCACCCGAAAAAACC‑AGTTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCAt                                                                      <  2:959030/225‑1 (MQ=255)

                                                                                                                                                                 aaCGAGTAATGAAGAAACTTTTTT‑A‑TGTGCATGAAGATTTAAT‑GCCTCCTCCTTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑AACAC‑ACTTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACC‑AGGTCACCGCCATTAAAAAAGCGCTATTCACCATTAACCATGGTGAGAATCTATTTATATCTTTCATACGCAATTAAACAATTCCCaa                                                                      >  1:1280265/1‑238 (MQ=255)

                                                                                                                                                                   cGAGTAATGAAGAAACTTTTTT‑A‑TGTGCATGAAGATTTAAT‑GCCTCCTCCTTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑AACAC‑ACTTATCTACTCGAATTTGGC‑TTGCATCCGCAAAACCC‑AGGTCACGGCCCTTAAAATAGCGCTATTCCCCATTAACACTGGTGAGAAAATATTCATTTCTTGCTAAAGAAATTAGACAATTCCAATga                                                                    >  1:913389/1‑238 (MQ=255)

                                                                                                                                                                          taaaaattttttttt‑A‑TTTACATGATTATTTACC‑CCCCCCCTCTTCAGCATGGGGAAAAAT‑AAT‑AAG‑ATGTTCTCCATGACTACAAAAAAAAATTTTA‑C‑AACAC‑ACTTATATACAGTATTTTTGT‑TTCCATCAAAAAAACCC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAG‑TGAtt                                                            <  1:1285586/230‑1 (MQ=255)

                                                                                                                                                                                 tttttttt‑A‑TTGGCATGAATTTTTAAC‑CCCTCCTCTTGGAGCATGGGAAAAAAT‑AAGGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTCT‑C‑AACAC‑TTTTATCTCCTCGAATTTGGC‑TTGCATCCACAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAG‑TGATTGCATAGt                                                     <  2:699988/237‑1 (MQ=255)

                                                                                                                                                                                 aCTTTTTT‑A‑TGTGCATGAAGATTTAAT‑GCCTCCTCCTTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑AACAC‑ACTTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGCATATATTTATGTCTTGAATAGGCAATTATACAATTCCAATGTAC‑GAATTCCATAGt                                                     >  2:1014665/1‑239 (MQ=255)

                                                                                                                                                                                   cttttttgaggggaataaaattttAAG‑CCCTCCGTCATGAGCATGGGAAGAAAG‑AAAAAAA‑GTGTTCCCTTTGAC‑AGGAAAAAAAAATTGT‑C‑ACAGC‑ATTTATCTACCCGATTTGTGC‑TTGACCCAGAAATAACC‑GGGCGACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAG‑TGATTGCATAGt                                                     <  2:880558/218‑1 (MQ=255)

                                                                                                                                                                                               ggcaggaaaTTTAATCGCCTCTCCTTTGACCAT‑GGGAAAAATAAATAAAG‑GCGTGTCCTTTGAC‑ACAAATA‑AAAATTTT‑C‑AAAACTATTTATC‑ACCTGAATTTTGG‑TTGCCACCCAAAAAACCAAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAG‑TGATTGCATAGTTGACTTAata‑t                                         <  2:947642/231‑1 (MQ=255)

                                                                                                                                                                                                   taaattttAAT‑GCCCCCTCCTGGAGAACGGGAAAAAAA‑AATGAAA‑GGGGGCCCCTTGAC‑AAAAATAAAAAATTTA‑C‑AACAC‑TCTTATCTACCCGAATTTGGC‑TTGCACCCAAAAAACCC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAG‑TGATTGCATAGTTGACTTAATA‑TTACAt                                    <  2:138100/234‑1 (MQ=255)

                                                                                                                                                                                                   gaatttttAAT‑CCCCCCTACTTGAACAGGGGGAAAAAT‑AAAGAAG‑GCGTCTCCCTT‑AC‑AAAAAAAAAAAATTTTAC‑AACCC‑TTTTATCTCCTAAAATTTGGC‑TTGCACCCAAAAAACCC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAG‑TGATTGCATAGTTGACTTAATA‑TTACAt                                    <  2:1226981/234‑1 (MQ=255)

                                                                                                                                                                                                   aaataTTTACT‑GCCCCCCCCTGGAGCA‑GGGGAAAAAA‑AAAGAAAGGCTTTCCCCTAGAC‑AAGAAAAAAAATTTTC‑C‑ACCCC‑ATTTTTCTACTCTATTTGGGC‑TTGCACCCAAAAAACCC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAG‑TGATTGCATAGTTGACTTAATA‑TTACAt                                    <  2:249728/235‑1 (MQ=255)

                                                                                                                                                                                                     agagTTAAC‑GCCTCGTCCTGGAGCATGGGGAAAAAT‑AAGAAAA‑GTGGCTCCTTAGAC‑A‑GAAAACAAAATTTTCC‑AACAT‑ATTTATCTACTAGATTTTGGC‑TTCCTCCCAAAAAAACC‑AGGTAGCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAG‑TGATTGCATAGTTGACTTAATA‑TTACATaa                                  <  2:239877/239‑1 (MQ=255)

                                                                                                                                                                                                        tttAAG‑CCCCCCGCCGTGAACAACGGGAAAAAA‑AAGAAAG‑GCTTCTCCCTTGAC‑ACAAAAAAAAATTTTC‑C‑AACAC‑ACTTATCTACCCAATTTGGGC‑TTGCACCCGAAAAAACA‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAG‑TGATTGCATAGTTGACTTAATA‑TTACATAAACa                               <  2:18165/239‑1 (MQ=255)

                                                                                                                                                                                                          tAAT‑GCCTCCTCCTTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGACCCTTGAC‑ACGAATACAAACTTTT‑C‑AACAC‑ACTTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATTCACCCTTAACCATGGTGAGATTATATTTATGTCTTGCATACGCAATCAAACAATTCCCAT‑TAGAGGTTTGCATAGtttcataaaaataaactaaaaaaa                              >  1:7115/1‑217 (MQ=255)

                                                                                                                                                                                                            aagcccgccgcatagAGCAGGGGGAAAAAT‑AATGAAC‑GCGTTACCATTGCA‑ACAAAAAAAAAATTTC‑A‑AACAC‑ACTTATCAACTGAAATTGGGC‑TTGCATCCAAAAAACCC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAG‑TGATTGCATAGTTGACTTAATA‑TTACATAAACatat                            <  2:51181/225‑1 (MQ=255)

                                                                                                                                                                                                                    ctcctTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑AACAC‑ACTTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAG‑t                                                                >  2:1230571/1‑196 (MQ=255)

                                                                                                                                                                                                                      ccatgagatggggaaaaaaa‑aaGGAAA‑GCTTTCCCCTAAAC‑ACAAAAACAAATTTTT‑C‑AAC‑C‑CCTTAACTTTCCAAAATTGGCTTTGCATCAACAAAAACC‑AGGTCTCCGCCATTAAAATAGCGATATACACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAG‑TGATTGCATAGTTGACTTAATA‑TTACATAAACATATTACTGTttat                  <  2:498476/223‑1 (MQ=255)

                                                                                                                                                                                                                               tCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑AACAC‑ACTTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAG‑TGATTGCATAGTTGACTTAata                                           >  2:47405/1‑206 (MQ=255)

                                                                                                                                                                                                                                cGGGAGAAAT‑AATAACA‑GCCTGTCCCTTGAC‑ACGCATACAAAATTTT‑C‑AACAC‑ACTTATCTACTCGACTTTGGC‑TTGCATCCGCCAAACCC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATCATCCATGTTGAGCATTTATTCATGTCTTGCATACGCAATTATACAATTCCCATGCAG‑TGATTGAATTGTTGATTTAAAA‑TTACATAAAAAAATACCTGTTATTTAAtatacc         >  1:218223/1‑236 (MQ=255)

                                                                                                                                                                                                                                 gggAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑AACAC‑ACTTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCCTGGTGAGAATCACTTTATGTCTTGCATACTCAATTAGACAATTCCCATGGAG‑TGATTGCATAGTTGAATTAATATTTACA‑AAAAAAATTTCTTTTTTTTAATGTAACa        >  1:868180/1‑238 (MQ=255)

                                                                                                                                                                                                                                      aaaT‑AAAGAAA‑GCTTTCCATTTCAC‑ACAAAAACAAATTTCA‑C‑AACTC‑CCTTACCCACTCAAATTTGCC‑TTGCCACCCAAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACTCAATTAGACAATTCCCATGTAG‑TGATTGCATAGTTGACTTAATA‑TTACATAAACATATTACTGTTTATTAATGTAGCAcgccgc  <  2:315135/239‑1 (MQ=255)

GTTCAGAAGCAGCGGTATAAAGTGCTCATCCTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAG‑CATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGG‑AATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTT‑A‑TGTGCATGAAGATTTAAT‑GCCTCCTCCTTGAGCATCGGGAGAAAT‑AATGAAA‑GCGTGTCCCTTGAC‑ACGAATACAAAATTTT‑C‑AACAC‑ACTTATCTACTCGAATTTGGC‑TTGCATCCGCAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAG‑TGATTGCATAGTTGACTTAATA‑TTACATAAACATATTACTGTTTATTAATGTAGCACGCCGC  >  NC_000913/3665399‑3665862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A