Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F9 I0 R1 142 103.7 2603848 81.8% 2129947 227.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,671,664 A→C 10.5% V77G (GTG→GGG)  yhjB ← putative DNA‑binding transcriptional response regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,671,6640AC10.5% 321.1 / 5.0 129V77G (GTG→GGG) yhjBputative DNA‑binding transcriptional response regulator
Reads supporting (aligned to +/- strand):  ref base A (55/56);  new base C (2/16);  total (57/72)
Fisher's exact test for biased strand distribution p-value = 2.01e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 130 total aligned reads displayed.

ACATGGTTAAAATCTCCCGCTGGCGGGCGCTTAACGATTTGAGATCTTTAATGTCCTTTTC‑CGGCGTCGTCCGCCAGTCTCCAGGCAGAAACATCATCCCCATCGCCGCA‑CTATTTACCGCCAACGCAAATGT‑CTCGACGGTTGAATCAC‑GAGGCACAATGGCCAGCACATTAAAATGGATAACTTCCTGTAACCACCGTTTATTGCAATCCGTCGCCGTAATTAACACCTTAACCTCAGGAAATTGCACCACGGTTTTTTGCAGCAACCAGTAGCAAAACTCACCATCCTGATCGCCATCGAGCATAACTAAGGCTTCAGGGTAACTTTCCAGCTTTTGCCATAACTCGTCTGCCTGACTGGCCCCCTGAATACTCACTCCTGGAATACGCTGCTGT‑AAACTGATTTTCATTCCATGAATAAATATTGACTGCCTGTCAAACATGACTATTTGCATAACTGAATC  >  NC_000913/3671437‑3671902
                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                               
       tAAAATCTCCCGCTGGCGGGCGCTTAACGATTTGAGATCTTTAATGTCCTTTTC‑CGGCGTCGTCCGCCAGTCTCCAGGCAGAAACATCATCCCCATCGCCGCA‑CTATTTACCGCCAACGCAAATGT‑CTCGACGGTTGAATCAC‑GAGGCACAATGGCCAGCACATTAAAATGGATAACTTCCTGTAACCACCGTTTATTGCAATCCGTCGCCGTAATTAACACCTTAACCTCAGGAAAtt                                                                                                                                                                                                                               >  2:855944/1‑239 (MQ=255)
             ctcCCGTTGGCGGGCGCTTAACGATTTGAGATCGTTAATGTCCTTTTC‑CGGCGTCGTCCGCCAGTCTCCAGGCAGAAACCTCATCCCCATCGCCGCA‑CTATTTACCGCCAACGCAAATGT‑CTCGACGGTTGAATCAC‑GAGGCACAATGGCCAGCACATTAAAATGGATAACTTCCTGTAACCACCGTTTATTGCAATCCGTCGCCGTAATTAACACCTTAACCTCAGGAAATTGcacca                                                                                                                                                                                                                         <  2:387843/239‑1 (MQ=255)
             cccccGCTGGCGGGCGCTTAACGATTTGAATATTTTAAGGTCCTTTTC‑CGGCGTCGTCCGCCAGTCTCCAGTCAGAAACATCATCCCCATCGCCGCA‑CTATTTACCGCCAACGCAAATGT‑CTCGACGGTTGAATCAC‑GAGGCACAATGGCCAGCACATTAAAATGGATAACTTCCTGTAACCACCGTTTATTGCAATCCGTCGCCGTAATTAACACCTTAACCTCAGGAAATTGcacca                                                                                                                                                                                                                         <  2:1257922/237‑1 (MQ=255)
             cccccGCTGGCGGGCCCTTAACGATTTGAGATCTTTAATGTCCTTTTC‑CGGCGTCGTCCGCCAGTCTCCAGGCAGAAACATCATCCCCATCGCCGCA‑CTATTTACCGCCAACGCAAATGT‑CTCGACGGTTGAATCAC‑GAGGCACAATGGCCAGCACATTAAAATGGATAACTTCCTGTAACCACCGTTTATTGCAATCCGTCGCCGTAATTAACACCTTAACCTCAGGAAATTGcacca                                                                                                                                                                                                                         <  2:460700/237‑1 (MQ=255)
                ccGCTGGCGGGCGCTTAACGATTTGAGATCTTTAATGTCCTTTTC‑CGGCGTCGTCCGCCAGTCTCCAGGCAGAAACATCATCCCCATCGCCGCA‑CTATTTACCGCCAACGCAAATGT‑CTCGACGGTTGAATCAC‑GAGGCACAATGGCCAGCACATTAAAATGGATAACTTCCTGTAACCACCGTTTATTGCAATCCGTCGCCGTAATTAACACCTTAACCTCAGGAAATTGCACCACgg                                                                                                                                                                                                                      >  2:879863/1‑239 (MQ=255)
                        ggggtcTTAACGTTTTGAGATTTTTAAGGTCCTTTTC‑CGGGTTCGTCCGCCAGTCTCCAGGCAGAAACATCATCCCCATCGCCGCA‑CTATTTACCGCCAACGCAAATGT‑CTCGACGGTTGAATCAC‑GAGGCACAATGGCCAGCACATTAAAATGGATAACTTCCTGTAACCACCGTTTATTGCAATCCGTCGCCGTAATTAACACCTTAACCTCAGGAAATTGCACCACGGTTTTTTg                                                                                                                                                                                                               <  2:1135665/233‑1 (MQ=255)
                          ccgcTTAACGATTGGATATCTTTAATGTCCTTTGC‑CGGCGTCGTCCGCCAGTCTCCAGGCAGAAACATCATCCCCATCGCCGCA‑CTATTTACCGCCAACGCAAATGT‑CTCGACGGTTGAATCAC‑GAGGCACAATGGCCAGCACATTAAAATGGATAACTTCCTGTAACCACCGTTTATTGCAATCCGTCGCCGTAATTAACACCTTAACCTCAGGAAATTGCACCACGGTTTTTTgca                                                                                                                                                                                                             <  2:594581/237‑1 (MQ=255)
                             cTTAACGATTTGAGATCTTTAATGTCCTTTTC‑CGGCGTCGTCCGCCAGTCTCCAGGCAGAAACATCATCCCCATCGCCGCA‑CTATTTACCGCCAACGCAAATGT‑CTCGACGGTTGAATCAC‑GAGGCACAATGGCCAGCACATTAAAATGGATAACTTCCTGTAACCACCGTTTATTGCAATCCGTCGCCGGAATTAAACCCTTAACCTCAGGAAATTGCACCACGGTTTTTTgcagca                                                                                                                                                                                                          >  2:770510/1‑238 (MQ=255)
                                        gggattcttAATGTCCTTTTC‑CGGCCTCGTCCGCCAGTCTCCAGGCCGAAACATCATCCCCATCGCCGCA‑CTATTTCCCGCCAACGCAAATGT‑CTCGACGGTTGAATCAC‑GAGGCACAATGGCCAGCACATTAAAATGGATAACTTCCTGTAACCACCGTTTATTGCAATCCGTCGCCGTAATTAACACCTTAACCTCAGGAAATTGCACCACGGTTTTTTGCAGCAACCAGTAGCaaa                                                                                                                                                                                              <  2:450044/232‑1 (MQ=255)
                                         agtattttctcttCCTTTTCGCGGCTTCCACCCCCAGGCCCCAGGCCTAAACTACATCCCCACCGCCCCA‑CTATTGTCCGCCAACGCAAATGT‑CTCGACGGTTGAATCAC‑GAGGCACAATGGCCAGCACATTAAAATGGATAACTTCCTGTAACCACCGTGTATTGCAATCCGTCGCCGTAATTAACACCTTAACCTCAGGAAATTGCACCACGGTTTTTTGCAGCAACCAGTAGCaaa                                                                                                                                                                                              <  1:797160/227‑1 (MQ=255)
                                               ttAATGTCCTTTTC‑CGGCGTCGTCCGCCAGTCTCCAGGCAGAAACATCATCCCCATCGCCGCA‑CTATTTACCGCCAACGCAAATGT‑CTCGACGGTTGAATCAC‑GAGGCACAATGGCCAGCACATTAAAATGGATAACTTCCTGTAACCACCGTTTATTGCAATCCGTCGCCGTAATTAACACCTTAACCTCAGGAAATTGCACCACGGTTTTTTGCAGCAACCAGTAGCAAAACTCAc                                                                                                                                                                                        >  2:1118797/1‑238 (MQ=255)
                                                  atttgcTTTTC‑CGGCTCCGCCCCGCAGTCTCCGGGCAGAAACGTCATCCCCATCGGCGCA‑CAATTTACCGCCAACGCAAATGT‑CTCGGCGGTTGAATCAC‑CAGGCACAATGGCCAGCACATTAAAATGGATAACTTCCTGTAACCACCGTTTATTGCAAGCCGTCGCCGTAATTAACACCTTAACCTCAGGAAATTGCACCACGGTTTTTTGCAGCAACCAGTAGCAAAACTCACCATc                                                                                                                                                                                    <  2:306325/234‑1 (MQ=255)
                                                    ctCCCTTCC‑CGACGTGCTCCCCCAGTCACCAGGGAGAAACATCACCCCAATCGCCCCC‑GTATTTACCGCCAACCCAAATGT‑CTCGACGGTTTAATCAC‑GAGTCACAATGGCCAGCACATTAAAATGGATAACTTCCTGTAACCACCGTTTATTGCAATCCGTCGCCGTAATTAACACCTTAACCTCAGGAAATTGCACCACGGTTCTTTGCAGCAACCAGTAGCAAAACTCACCATCCt                                                                                                                                                                                  <  1:913768/238‑1 (MQ=255)
                                                       cTTTTC‑CGGCGTCGTCCGCCAGTCTCCAGGCAGAAACATCATCCCCATCGCCGCA‑CTATTTACCGCCAACGCAAATGT‑CTCGACGGTTGAATCAC‑GAGGCACAATGGCCAGCACATTAAAATGGATAACTTCCTGTAACCACCGTTTATTGCAATCCGTCGCCGTAATTAACACCTTAACCTCAGGAAATTGCACCACGGTTTTTTGCAGCAACCAGTAGCAAAACTCACCATCCTGa                                                                                                                                                                                >  2:747330/1‑238 (MQ=255)
                                                       cTTTTC‑CGGCGTCGTCCGCCAGTCTCCAGGCAGAAACATCATCCCCATCGCCGCA‑CTATTTACCGCCAACGCAAATGT‑CTCGACGGTTGAATCAC‑GAGGCACAATGGCCAGCACATTAAAATGGATAACTTCCTGTAACCACCGTTTATTGCAATCCGTCGCCGTAATTAACACCTTAACCTCAGGAAATTGCACCACGGTTTTTTGCAGCAACCAGTAGCAAAACTCACCATCCTGa                                                                                                                                                                                >  2:207895/1‑238 (MQ=255)
                                                       cTTTTC‑CGGCGTCGTCCGCCAGTCTCCAGGCAGAAACATCATCCCCATCGCCGCA‑CTATTTACCGCCAACGCAAATGT‑CTCGACGGTTGAATCAC‑GAGGCACAATGGCCAGCACATTAAAATGGATAACTTCCTGTAACCACCGTTTATTGCAATCCGTCGCCGTAATTAACACCTTAACCTCAGGAAATTGCACCACGGTTTTTTGCAGCAACCAGTAGCAAAACTCACCATCCTGa                                                                                                                                                                                >  2:595542/1‑238 (MQ=255)
                                                            c‑cGGGGTCCTCCGCCAGTCTCAAGGCAGAAACATCATCCCCATCGCCGCA‑CTATTTACCGCCAACGCAAATGT‑CTCGACGGTTGAATCAC‑GAGGCACAATGGCCAGCACATTAAAATGGATAACTTCCTGTAACCACCGTTTATTGCAATCCGTCGCCGTAATTAACACCTTAACCTCAGGAAATTGCACCACGGTTTTTTGCAGCAACCAGTAGCAAAACTCACCATCCTGATCGCCa                                                                                                                                                                          <  2:573100/239‑1 (MQ=255)
                                                             ccctcgtccttgctcttgctcCCGGCAGAAACAACCCCCCCTTCACAGCA‑CAATTTCACGCCAAC‑CAAAAGTACTCGACGGTTGAAACCC‑GAGGCCCAATGGCCAGCACATTAAAATTGATAACTTCCTGTAACCACCTTTTATTGCAATCCGTCGCCGTAATTAACACCTTAACCTCAGGAAATTGCACCTCGGTTTTTTGCAGCATCCAGTAGCAAAACTCACCATCCTGATCGCCa                                                                                                                                                                          <  1:1042460/221‑1 (MQ=255)
                                                              tggctacggtccccactgtcCAGGCAGAAACACCA‑CCCCCTCCCCCCATCTATTTCCCCCCAAAGAAAATGT‑TGCGGCGGTTGAATCCC‑GAGCCACAATGGCCAGCACATTAAAATGGATAACTTACTGTAACCCCCGTTTATTCCAATCCGTCGCCGTAATTAACACCTTAACCTCAGGAAATTGCACCACGGTTTTTTGCAGCAACCAGTAGCAAAACTCACCATCCTGATCGCCAt                                                                                                                                                                         <  1:35740/221‑1 (MQ=255)
                                                                gCGTCGTCCGCCAGTCTCCAGGCAGAAACATCATCCCCATCGCCGCA‑CTATTTACCGCCAACGCAAATGT‑CTCGACGGTTGAATCAC‑GAGGCACAATGGCCAGCACATTAAAATGGATAACTTCCTGTAACCACCGTTTATTGCAATCCGTCGCCGTAATTAACACCTTAACCTCCGGAAATTGCACCCCGGTTTTTTGCAGCAACCACAGGAAAAACCACCCATCCTAATCCCCaccc                                                                                                                                                                       >  1:67249/1‑236 (MQ=255)
                                                                gCGTCGTCCGCCAGTCTCCAGGCAGAAACATCATCCCCATCGCCGCA‑CTATTTACCGCCAACGCAAATGT‑CTCGACGGTTGAATCAC‑GAGGCACAATGGCCAGCACATTAAAATGGATAACTTCCTGTAACCACCGTTTATTGCAATCCGTCGCCGTAATTAACACCTTAACCTCAGGACATTGCACCACGCGTTTTTGCACCAACCACTCGAAAAACCCACCACCCTgtacccactcg                                                                                                                                                                       >  1:235129/1‑229 (MQ=255)
                                                                         gCCAGTCTCCAGGCAGAAACATCATCCCCATCGCCGCA‑CTATTTACCGCCAACGCAAATGT‑CTCGACGGTTGAATCAC‑GAGGCACAATGGCCAGCACATTAAAATGGATAACTTCCTGTAACCACCGTTTATTGCAATCCGTCGCCGTAATTAACACCTTAACCTCAGGAAATTGCACCACGGTTTTTTGCAGCAACCAGTAGCAAAACTCACCATCCTGATCGCCATCGAGCATAACt                                                                                                                                                              <  2:256968/239‑1 (MQ=255)
                                                                           cAGTCCCCAGGCAAAAACATCATCCCCATCGCCGCA‑CTATTTACCGGCAACGCAAATGT‑CTCGACGGTTGAATCAC‑GAGGCACAATGGCCAGCACATTAAAATGGATAACTTCCTGTAACCACCGTTTATTGCAATCCGTCGCCGTAATTAACACCTTAACCTCAGGAAATTGCACCACGGTTTTTTGCAGCAACCAGTAGCAAAACTCACCATCCTGATCGCCATCGAGCATAACTaa                                                                                                                                                            <  2:62307/239‑1 (MQ=255)
                                                                             gTCTCCAGGCAGAAACATCATCCCCATCGCCGCA‑CTATTTACCGCCAACGCAAATGT‑CTCGACGGTTGAATCAC‑GAGGCACAATGGCCAGCACATTAAAATGGATAACTTCCTGTAACCACCGTTTATTGCAATCCGTCGCCGTAATTAACACCTTAACCTCAGGAAATTGCACCACGGTTTTTTGCAGCAACCAGTAGCAAAACTCACCATCCTGATCGCCATCGAGCATAACTAAgg                                                                                                                                                          >  2:1177565/1‑239 (MQ=255)
                                                                              tctcCAGGCAGAAACATCATCCCCATCGCCGAA‑CTATTTACCGCCAACGCAAATGT‑CTCGACGGTTGAATCAC‑GAGGCACAATGGCCAGCACATTAAAATGGATAACTTCCTGTAACCACCGTTTATTGCAATCCGTCGCCGTAATTAACACCTTAACCTCAGGAAATTGCACCACGGTTTTTTGCAGCAACCAGTAGCAAAACTCACCATCCTGATCGCCATCGAGCATAACTAAg                                                                                                                                                           >  2:392478/1‑237 (MQ=255)
                                                                                      cagaaccacttcccacATTGCCACA‑CTCTCTACCGCAAAGGCAAATGT‑CTCGAAGGTTGAAGCACGGAGACA‑AAGTGCCAGCCCATTAAAATGGATAACGTCCTGGAACCACCGTTTAGTGCAATCCGTCGCCGTAATTAACACCTTAACCTCAGGAAATTGCCCCACGGTTTTTTGCAGCAACCAGTAGCAAAACTCACCATCCTGATCGCCATCGAGCATAACTAAGGCTTCAGGGt                                                                                                                                                 <  1:814149/224‑1 (MQ=255)
                                                                                      cAGAAACATCATCCCCATCGCCGCA‑CTTTTTACCGCCAACGCAAATGT‑CTCGACGGTTGAATCAC‑GAGGCACAATGGCCAGCACATTAAAATGGATAACTTCCTGTAACCACCGTTTATTGCAATCCGTCGCCGTAATTAACACCTTAACCTCAGGAAATTGCACCACGGTTTTTTGCAGCAACCAGTAGCAAAACTCACCATCCTGATCGCCATCGAGCATAACTAAGGCTTCAg                                                                                                                                                    <  2:158312/236‑1 (MQ=255)
                                                                                       aaaaaCCTCCTCCCCATCGCCGCA‑TTAATTACCGCCAACGCAAATGG‑CTCGACGGTTGAATCAC‑GAGGCCCAATGGCCAGCACATTAAAATGGATAACTTCCTGTAACCACCGTTTATTGCAATCCGTCGCCGTAATTAACACCTTAACCTCAGGAAATTGCACCACGGTTTTTTGCAGCAACCAGTAGCAAAACTCACCATCCTGATCGCCATCGAGCATAACTAAGGCTTCAGGGt                                                                                                                                                 <  2:1029037/236‑1 (MQ=255)
                                                                                       aGAAACCTCATCCCCATCGCCGCA‑CTATTTACCGCCAACGCAAATGT‑CTCGACGGTTGAATCAC‑GAGGCACAATGGCCAGCACATTAAAATGGATAACTTCCTGTAACCACCGTTTATTGCAATCCGTCGCCGTAATTAACACCTTAACCTCAGGAAATTGCACCACGGTTTTTTGCAGCAACCAGTAGCAAAACTCACCATCCTGATCGCCATCGAGCATAACTAAGGCTTCAGGGt                                                                                                                                                 <  2:863053/238‑1 (MQ=255)
                                                                                         aaaCATCATCCCCATCGCCGCA‑CTATTTACCGCCAACGCAAATGT‑CTCGACGGTTGAATCAC‑GAGGCACAATAGCCAGCACATTAAAATGGATAACTTCCTGTAACCACCGTTTATTGCAATCCGTCGCCGTAATTAACACCTTAACCTCAGGAAATTGCACAACGGTTTTTTTCAGCAAACAGAAGCAACACTCACCATCCTGATCGCCATAGAGCAAAACAAAGGCTTCGGGGTaaa                                                                                                                                              >  1:151482/1‑238 (MQ=255)
                                                                                                       tCGCCGCA‑CTATTTACCGCCAACGCAAATGT‑CTCGACGGTTGAATCAC‑GAGGCACAATGGCCAGCACATTAAAATGGATACCTTCCTGTAACCACCGTTTATTGCAATCCGTCGCCGTAATTAACACCTTAACCTCAGGAAATTGCACCCCGGTTTTTTGCAGCAACAAGTAGCAAAACTCCCCATCCTGACCACCATCCAGCAGAACTAAGGATCACGGGAAACTTTCCAGCTTTTg                                                                                                                                 >  1:1130884/1‑238 (MQ=255)
                                                                                                       tCGCCGCA‑CTATTTACCGCCAACGCAAATGT‑CTCGACGGTTGAATCAC‑GAGGCACAATGGCCAGCACATTAAAATGGATAACTTCCTGTAACCACCGTTTATTGCAATCCGTCGCCGTAATTAACACCTTAACCTCAGGAAATTGCACCACGGTTTTTTGCAGCAACCAGTAGCAAAACTAACCATCCTGATCGCCATCGAGCATAACTAAGGCTTCAGGGTAACTTTCCAGCTTTTg                                                                                                                                 >  2:1104198/1‑238 (MQ=255)
                                                                                                             cA‑CTATTTACCGCCAACGCAAATGT‑CTCGACGGTTGAATCAC‑GAGGCACAATGGCCAGCACATTAAAATGGATAACTTCCTGTAACCACCGTTTATTGCAATCCGTCGCCGTAATTAACACCTTAACCTCAGGAAATTGCACCACGGTTTTTTGCAGCAACCAGTAGCCAAACTCACCATCCTGATCCCCCACCCGCATAACTAAGGCTCACGGGAAACTTTCCAACTTTTGCCATaa                                                                                                                           >  1:1220773/1‑238 (MQ=255)
                                                                                                                     taccccccacaaaaaTGT‑CACCACCGTTGAATCAC‑GGGGCACAATGGCCAGCACATTAAAATGGATAACTCCCGGTAACCACCGTTTATTGCAAGCCGTCGCCGTAATTAACCCCGTAACCTCAGGAAATTGCACCACGGTTTTTTGCAGCAACCAGTAGCAAAACTCACCATCCTGATCGCCATCGAGCAGAACTAAGGCTTCAGGGTAACTTTCCAGCTTTTGCCATAACTCGTCTg                                                                                                                   <  1:1290031/227‑1 (MQ=255)
                                                                                                                      aCCGCCAACGCAAATGT‑CTCGACGGTTGAATCAC‑GAGGCACAATGGCCAGCCCATTAAAATGGATAACTTCCTGTAACCACCGTTTATTGCAATCCGTCGCCGTAATTAACACCTTAACCTCAGGAAATTGCACCACGGTTTTTTGCAGCAACCAGTAGCAAAACTCa                                                                                                                                                                                         <  1:1090882/168‑1 (MQ=255)
                                                                                                                      aCCGCCAACGCAAATGT‑CTCGACGGTTGAATCAC‑GAGGCACAATGG‑CAGCACATTAAAATGGATAACTTCCTGTAACCACCGTTTATTGCAATCCGTCGCCGTAATTAACACCTTAACCTCAGGAAATTGCACCACGGTTTTTTGCAGCAACCAGTAGCAAAACTCACCATCCTGATCGCCATCGAGCATAACTAAGGCTTCAGGGTAACTTTCCAGCTTTTGCCATAACTCGTCTg                                                                                                                   >  2:903875/1‑237 (MQ=255)
                                                                                                                        cGCCAACGCAAATGT‑CTCGACGGTTGAATCAC‑GAGGCACAATGGCCAGCACATTAAAATGGATAACTTCCTGTAACCACCGTTTATTGCAATCCGTCGCCGTAATTAACACCTTAACCTCAGGAACTTGCACCACGGTTTTTTGCAGCAACCAGTAGCAAAACTCACCATCCTGATCGCCATCGAGCATAACTAAGGCTTCAGGGTAACTTTCCAACTTTTGCCATAACTctcttgcc                                                                                                                 >  2:44296/1‑231 (MQ=255)
                                                                                                                             aCGCAAATGT‑CTCGACGGTTGAATCAC‑GAGGCACAGTGGCCAGCACATTAAAATGGATAACTTCCTTTAACCACCGTTTATTGCGATACGTCGCCGTAATTAACCCCTTAACCTCAGTAAATTTCACCACGGTTTTTTGCATCAACCAGAAGCAAAACTCACCATCCTGAACGCCACCGAGCAGAACTAAGGTTTAAGGGTAACTTCAAACCTTTTCCCATaaaccgcccaccagacg                                                                                                            >  1:1171757/1‑223 (MQ=255)
                                                                                                                             aCGCAAATGT‑CTCGACGGTTGAATCAC‑GAGGCACAATGGCCAGCCCATTAAAATGGCTAACTTCCTGTAACCACCGTTTATTGCAATCCGCCGCCGCAATTACCACCTTAACCTCAGGCAATTGCACCACGGGTCTTTGCAGCAACCACTAGCAAAACTCAACATCCTTGTAGCCACCTCGCGTAACCAAAGCTTTAGGGTAACTTTCCCACTTTTGcacaaacacctttgccagaccg                                                                                                           >  1:1018375/1‑218 (MQ=255)
                                                                                                                             aCGCAAATGT‑CTCGACGGTTGAATCAC‑GAGGCACAATGGCCAGCACATTAAAATGGATAACTTCCTGTAACCACCGTTTATTGCAATCCGTCGCCGTAATTAACACCTTAACCTCAGGAAATTGCACCACGGTTTTTTGCAGCAACCAGTAGCAAAACTCACCATCCTGATCGCCATCGAGCATAACTAAGGCTTCAGGGTAACTTTCCAGCTTTTGCCATAACTCGTCTGCCTGACt                                                                                                            >  2:376413/1‑238 (MQ=255)
                                                                                                                             aCGCAAATGT‑CTCGACGGTTGAATCAC‑GAGGCACAATGGCCAGCACATTAAAATGGATAACTTCCTGTAACCACCGTTTATTGCAATCCGTCGCCGTAATTAACACCTTAACCTCAGGAAATTGCACCACGGTTTTTTGCAGCAACCAGTAGCAAAACTCACCATCCTGATCGCCATCGAGCATAACTAAGGCTTCAGGGTAACTTTCCAGCTTTTGCCATAACTCGTCTGCCTGACt                                                                                                            >  2:294378/1‑238 (MQ=255)
                                                                                                                             aCGCAAATGT‑CTCGACGGTTGAATCAC‑GAGGCACAATGGCCAGCACATTAAAATGGATAACTTCCTGTAACCACCGTTTATTGCAATCCGTCGCCGTAATTAACACCTTAACCTCAGGAAATTGCACCACGGTTTTTTGCAGCAACCAGTAGCAAAACTCACCATCCTGATCGCCATCGAGCATAACTAAGGCTTCAGGGTAACTTTCCAGCTTTTGCCATAACTCGTCTGCCTGACt                                                                                                            >  2:344098/1‑238 (MQ=255)
                                                                                                                               gCAAATGT‑CTCGACGGTTGAATCAC‑GAGGCACAATGGCCAGCACATTAAAATGGATAACTTCCTGTAACCCCCGTTTATTGCAATCCGTCGCCGTAATTAACACCTTAACCTCAGGAAATTGCACCACGGTTTTTTGCAGCAACCAGTAGCAAAACTCACCATCCTGATCGCCATCGAGCATAACTAAGGCTTCAGGGTAACTTTCCAGCTTTTGCCATAACTCGTCTGCCTGACTGGc                                                                                                         <  2:1105165/239‑1 (MQ=255)
                                                                                                                               gCAAATGT‑CTCGACGGTTGAATCAC‑GAGGCACAATGGCCAGCACATTAAAATGGATAACTTCCTGTAACCACCGTTTATTGCAATCCGTCGCCGTAATTAACACCTTAACCTCAGGAAATTGCACCACGGTTTTTTGCAGCAACCAGTAGCAAAACTCACCATCCTGATCGCCATCGAGCATAACTAAGGCTTCAGGGTAACTTTCCAGCTTTTGCCATAACTCGTCTGCCTGACTGGc                                                                                                         <  2:289952/239‑1 (MQ=255)
                                                                                                                                 aaaTGT‑CTTAACGGTTTAAACCC‑GAGGCACAATGGCCAGCACATTAAAATGTATAACTTCCTGTAACCACCGTTTATTGCAATCCGTCGCCGTAATTAACCCCTTAACCTCAGGAAATTGCACCACGGTTTTTTGCAGCAACCAGTAGCAAAACTCACCATCCTGATCGCCATCGAGCATAACTAAGGCTTCAGGGTAACTTTCCAGCTTTTGCCATAACTCGTCTGCCTGACTGGc                                                                                                         <  2:1147785/237‑1 (MQ=255)
                                                                                                                                  aaTGT‑CTCGACGGTTGAATCAC‑GAGGCACAATGGCCAGCACATTAAAATGGATAACTTCCTGTAACCACCGTTTATTGCAATCCGTCGCCGTAATTAACACCTTAACCTCAGGAAATTGCACCACGGTTTTTTGCAGCAACCAGTAGCAAAACTCACCATCCTGATCGCCATCGAGCATAACTAAGGCTTCAGGGTAACTTTCCAGCTTTTGCCATAACTCGTCTGCCTGACTGGcccc                                                                                                      <  2:151482/239‑1 (MQ=255)
                                                                                                                                       gatcgtctggtGAATACC‑GAGGCACAATGGCCAGCACATTAAAATGGATAACCTCCTGTAACCACCGTTTATTGCAATCCGTTGCCGTAATTAACCCCTTAACCTCAGGAAATTGCACCACGGTTTTTTGCAGCAACCAGTAGCAAAACTCACCATCCTGATCGCCATCGAGCATAACTAAGGCTTCAGGGTAACTTTCCAGCTTTTGCCATAACTCGTCTGCCTGACTGGCCCCCt                                                                                                    <  2:473868/227‑1 (MQ=255)
                                                                                                                                        ctctcctgTTGAATCAC‑GAGGCCAAATGGCCAGCCCAGTAAAAGGGATACCACCCAGTAACCCCCGTTTAGTGCAATCCGTCGCCGTAATTAACCCCTTAACCTCAGGAAATGGCACCACGGTTTTTTGCAGCAACCAGTAGCAAAACTCACCATCCTGATCGCCATCGAGCATAACTAAGGCTTCAGGGTAACTTTCCAGCTTTTGCCATAACTCGTCTGCCTGACTGGCCCCCTGaa                                                                                                 <  1:879863/232‑1 (MQ=255)
                                                                                                                                            aaggTTGAACCAC‑GAGGCCCAATGGCCAGCCCATTAAAATGGATAACTTCCCGTAACCACCGTTTATTGGCATCCGTCGCCCTAATTAACACCTTAACCTCAGGAAATTGCACCACGGTTTTTTGCAGCAACCAGTAGCAAAACTCACCATCCTGATCGCCATCGAGCATAACTAAGGCTTCAGGGTAACTTTCCAGCTTTTGCCATAACTCGTCTGCCTGACTGGCCCCCTGAATact                                                                                             <  2:1211997/237‑1 (MQ=255)
                                                                                                                                            aCGTTTTAATCAC‑CAGGCCTAATGGCCCGAATATTAAAATGGATAACTCCCTGTAACCACCGTTTATTGCAATCCGTTGCCGTAATTAACCCCTTAACCTCAGGAAATTGCACCACGGTTTTTTGCAGCAACCAGTAGCAAAACTCACCATCCTGATCGCCATCGAGCATAACTAAGGCTTCAGGGTAACTTTCCAGCTTTTGCCATAACTCGTCTGCCTGACTGGCCCCCTGAATact                                                                                             <  1:207895/239‑1 (MQ=255)
                                                                                                                                            aCGGTTGAATCAC‑GAGGCCCAATTGCCAGCACATTAAAATGGATAACTTCCTGTAACCACCGTTTATTGCAATCCGTCGCCGTAATTAACACCTTAACCTCAGGAAATTGCACCACGGTTTTTTGCAGCAACCAGTAGCAAAACTCACCATCCTGATCGCCATCGAGCATAACTAAGGCTTCAGGGTAACTTTCCAGCTTTTGCCATAACTCGTCTGCCTGACTGGCCCCCTGAATact                                                                                             <  2:692684/239‑1 (MQ=255)
                                                                                                                                             cGGTTGAATCAC‑GAGGCACAATGGCCAGCACATTAAAATGGATAACTTCCTGTAACCACCGTTTATTGCAATCCGTCGCCGTAATTAACACCTTAACCTCAGGAAATTGCACCACGGTTTTTTGCAGCAACCAGTAGCAAAACTCACCATCCTGATCGCCATCGAGCATAACTAAGGCTTCAGGGTAACTTTCCAGCTTTTGCCATAACTCGTCTGCCTGACTGGCCCCCTGAATact                                                                                             <  2:1290391/238‑1 (MQ=255)
                                                                                                                                             cGGTTGAATCAC‑GAGGCACAATGGCCAGCACATTAAAATGGATAACTTCCTGTAACCACCGTTTATTGCAATCCGTCGCCGTAATTAACACCTTAACCTCAGGAAATTGCACCACGGTTTTTTGCAGCAACCAGTAGCAAAACTCACCATCCTGATCGCCATCGAGCATAACTAAGGCTTCAGGGTAACTTTCCAGCTTTTGCCATAACTCGTCTGCCTGACTGGCCCCCTGAATact                                                                                             <  2:539853/238‑1 (MQ=255)
                                                                                                                                              gtcagatttttataagagacgtGGCCATCCCATTAAAATGGAAAACTTCCTTGAACCACCGTTTATTGCAAGCCGTCGCCGTAATTAACACCTTAACCTCAAGAAATTGCACCACGGTTTTTTGCAGCAACCAGTAGCAAAACTCACCATCCTGATCGCCATCGAGCATAACTAAGGCTTCAGGGTAACTTTCCAGCTTTTGCCATAACTCGTCTGCCTGACTGGCCCCCTGAATact                                                                                             <  1:186264/217‑1 (MQ=255)
                                                                                                                                                          gAGGCACAATGGCCAGCCCATTAAAATGGATAACTTCCTGTAACCACCGTTTATTGCAATCCGTCGCCGTAATTAACACCTTAACCTCAGGAAATTGCACCACGGTTTTTTGCAGCAACCAGTAGCAAAACTCACCATCCTGATCGCCATCGAGCATAACTAAGGCTTCAGGGTAACTTTCCAGCTTTTGCCATAACTCGTCTGCCTGACTGGCCCCCTGAATACTCACTCCTGGAATa                                                                                <  2:13250/239‑1 (MQ=255)
                                                                                                                                                          gAGGCAAAATGGCCAGCACCTTAAAATGGATAACTTCCTGTAACCACCGTTTATTGCAATCCGTCGCCGTAATTAACACCTTAACCTCAGGAAATTGCACCACGGTTTTTTGCAGCAACCAGTAGCAAAACTCACCATCCTGATCGCCATCGAGCATAACTAAGGCTTCAGGGTAACTTTCCAGCTTTTGCCATAACTCGTCTGCCTGACTGGCCCCCTGAATACTCACTCCTGGAATa                                                                                <  2:1171757/239‑1 (MQ=255)
                                                                                                                                                           aGGCACAATGGCCAGCACATTAAAATGGATAACTTCCTGTAACCACCGTTTATTGCAATCCGTCGCCGTAATTAACACCTTAACCTCAGGAAATTGCACCACGGTTTTTTGCAGCAACCAGTAGCAAAACTCACCATCCTGATCGCCATCGAGCATAACTAAGGCTTCAGGGTAACTTTCCAGCTTTTGCCCTAACTCGTCTGCCTGACTGGCCCCCTGAATACTCCCTCCTGGAATa                                                                                >  2:337880/1‑238 (MQ=255)
                                                                                                                                                            gggataggggCCACCACATTAAAATGTATAACTTCTTTTAACCACAGTTTATGGCAATCCGTCGCCGTAATTAACCCCTTAACCTCAGGAAATTGCACCACGGTTTTTTGCAGCAACAAGTAGCAAAACTCACCATCCTGATCGCCATCGAGCATAACTAAGGCTTCAGGGTAACTTTCCAGCTTTTGCCATAACTCGTCTGCCTGACTGGCCCCCTGAATACTCACTCCTGGAATACg                                                                              <  1:48462/231‑1 (MQ=255)
                                                                                                                                                            ggCCCATTGGCCAGCACATTAAAATGGATATCTTCCTGTAACCACCTTTTATTGCAATCCGTCGCTGTAATTAACACCTTACCCTCAGGAAATTTCCCCACGGTTTTTTGCAGCAACCAGTAGCAAAACTCACCATCCTGATCGCCATCGAGCATAACTAAGGCTTCAGGGTAACTTTCCAGCTTTTGCCATAACTCGTCTGCCTGACTGGCCCCCTGAATACTCACTCCTGGAATACg                                                                              <  1:879821/239‑1 (MQ=255)
                                                                                                                                                            gcaccaATTGCCAGCGTATGAAAATGGATATCTGCCTGTAACCACCGTTTATTGCCCACCGTCGCTGTAATTAACACCTTAACCTCAGGAAATTGCACCCCGGTTTTTTGCAGCAACCAGTAGCAAAACTCACCATCCTGATCGCCATCGAGCATAACTAAGGCTTCAGGTTAACATTCCAGCTTTTGCCATAACTCGTCTGCCTGACTGGCCCCCTGAATACTCACTCCTGGAATACg                                                                              <  1:709747/235‑1 (MQ=255)
                                                                                                                                                               acacTGGCCAGCACATTAAAATGGATACCTTCCTGTAACCACCGTTTATTGCAATCCGTCGCCGTAATTAACACCTTAACCTCAGCAAATTGCACCACGGTTTTTTGCAGCAACCAGTAGCAAAACTCACCATCCTGATCGCCATCGAGCATAACTAAGGCTTCAGGGCAACTTTCCAGCATTTGCCATAACTCGCCCTCCTGGCTGTCCCCCTACATACCCAACCATGAAAAACgct                                                                            >  1:327404/1‑238 (MQ=255)
                                                                                                                                                                caATGGCCAGCACATTAAAATGGATAACTTCCTGTAACCACCGTTTATTGCAATCCGTCGCCGTAATTAACACCTTAACCTCAGGAAATTGCACCACGGTTTTTTGCAGCAACCAGTAGCAAAACTCACCATCCTGATCGCCATCGAGCATAACTAAGGCTTCAGGGTAACTTTCCAGCTTTTGCCATAACTCGTCTGCCTGACTGGCCCCCTGAATa                                                                                               <  2:9648/218‑1 (MQ=255)
                                                                                                                                                                     gCCAGCACATTAAAATGGATAACTTCCTGTAACCACCGTTTATTGTAATCCGTCGCCGTAATTAACACCTTAACCTCAGGAAATTGCACCACGGTTTTTTGCAGCAACCAGTAGCAAAACTCACCATCCTGATCGCCATCGAGCATAACTAAGGCTTCAGGGTAACTTTCCAGCTTTTGCCATAACTCGTCTGCCTGACTGGCCCCCTGAATact                                                                                             <  2:1034491/215‑1 (MQ=255)
                                                                                                                                                                      cccgCACATTAAAATGGATAACTTCATGTAACCACCGTTTATTGCACTCAGTCGCCGTAATTAACACCTTAACCACAGGAAATTGCACCACGGTTTTTGGCAGCAACAAGTAGCAAAACTCAACATCCTGATCGCCATCGAGCTTAACTAAGGCTTCAGGGTAACTTTCCAGCTTTTGCCATAACCCGTCTGCGTGTATCGCCCCCTGATAACTCACTCCTGGAAtcggccgttgcaa                                                                     >  1:237001/4‑226 (MQ=255)
                                                                                                                                                                        aGCACATTAAAATGGATAACTTCCTGTAACCACCGTTTATTGCAATCCGTCGCCGTAATTAACACCTTAACCTCAGGAAATTGCACCACGGTTTTTTGCAGCAACCAGTAGCAAAACTCACCATCCTGATCGCCATCGAGCATAACTAAGGCTTCAGGGTAACTTTCCAGCTTTTGCCATAACTCGTCTGCCTGACTGGCCCCCTGACTAATCACTCCTGGAATACGCGGCAGT‑aa                                                                    >  1:116307/1‑236 (MQ=255)
                                                                                                                                                                               tAAAATGGATAACTTCCTGTAACCACCGTTTATTGTAATCCGTCGCCGTAATTAACACCTTAACCTCAGGAAATTGCACCACGGTTTTTTGCAGCAACCAGTAGCAAAACTCACCATCCTGATCGCCATCGAGCATAACTAAGGCTTCAGGGTACCTTTCCAGCTTTTGCCATAAATCGCCTGCCTGACTGGCCCCCTGAATACCACCCCCTGGaactgtcccttctacacacctgac                                                            >  1:1034491/1‑216 (MQ=255)
                                                                                                                                                                               tAAAATGGATAACTTCCTGTAACCACCGTTTATTGCAATCCGTCGCCGTAATTAACACCTTAACCTCAGGAAATTGCACCACGGTTTTTTGCAGCAACCAGTAGCAAAACTCACCATCCTGATCGCCATCGAGCATAACTAAGGCTTCAGGGTAACTTTCCAGCTTTTGCCATAACTCGTCTGCCTGACTGGCCCCCTGAATACTCACTCCTGGAATACGCTCCTGT‑aaa                                                                   >  2:48462/1‑230 (MQ=255)
                                                                                                                                                                               tAAAATGGATAACTTCCTGTAACCACCGGTTATTGCAATCCGTCGCCGTAATTAACACCTTAACCTCAGGAAATTGCACCACGGTTTTTTGCAGCAACCAGTAGCAAAACTCACCATCCTGATCGCCCTCGAGCATAACTAAGGCTTCAGGGTAACTTTCCAGCTTTTTCCATAACTCTTCTGCCTGCCTGGCCCCCTGCATACTCCCTCCTGGAAAActcgcctgaaaaaagtttttc                                                           >  1:1203514/1‑219 (MQ=255)
                                                                                                                                                                                    tGGATAACTTCCTGTAACCCCCGTTTATTGCAATCCGTCGCCGTAATTAACACCTTAACCTCAGGAAATTGCACCACGGTTTTTTGCAGCAACCAGTAGCAAAACTCACCATCCTGATCGCCATCGAGCATAACTAAGGCTTCAGGGTAACTTTCCAGCTTTTGCCATAACTCGTCTGCCTGACTGGCCCCCTGAATACTCACTCCTGGAATACGCTGCTGT‑AAACTGATTTTCATTcc                                                     <  2:235129/239‑1 (MQ=255)
                                                                                                                                                                                    tGGATAACTTCCTGTAACCCCCGTTTATTGCAATCCGTCGCCGTAATTAAAAACTTAACCTCAGGAAATTGAACCACGGTTTTTTGCAGCAACCAGTAGCAAAACTCACCATCCTGATCGCCATAGAGCATAACTAAGGCTTCAGGGTAAATTTCCAGCTTTTGCCAAACATCGTTTACCTGACTGGCCCCCTGAATACCCACTCCTGGAATAGACACCTGT‑AAACTGACTTTAATTcc                                                     >  1:651334/1‑239 (MQ=255)
                                                                                                                                                                                     tgATAACTTCTTGTAACCTCCGTTTAGTGCAATCCTTTCCCGTAAATAACACCTTAACCTCAGGAAATTGCCCCACTGTTTTTTGCAGCCACCAGTAGCAAAACTCCCCATCCTGATCGCCATCGAGCATAACTAAGGCTTCAGGGTAACTTTCCAGCTTTTGCCATAACTCGTCTGCCTGACTGGCCCCCTGAATACTCACTCCTGGAATACGCTGCTGT‑AAACTGATTTTCATTCCa                                                    <  1:242539/238‑1 (MQ=255)
                                                                                                                                                                                       aTAACTTCCTGTAACCACCGTTTATTGCAATCCGTCGCCGTAATTAACACCTTAACCTCAGGAAATTGCACCACGGtttt                                                                                                                                                                                                                  <  1:571767/80‑1 (MQ=255)
                                                                                                                                                                                       aTAACTTCCTGTAACCACCGTTTATTGCAATCCGTCGCCGTAATTAACACCTTAACCTCAGGAAATTGCACCACGGtttt                                                                                                                                                                                                                  >  2:571767/1‑80 (MQ=255)
                                                                                                                                                                                         aaCTTCCTGTAACCCCCGTTTATTGCCATCCGTCGCCGTAAGTAACCCCTTAACCTCAGGAAATTGCACCACGGTTTGTTGCAGCAACCAGTAGCAAAACTCACCATCCTGATCGCCATCGAGCATAACTAAGGCTTCAGGGTAACTTTCCAGCTTTTGCCATAACTCGTCTGCCTGACTGGCCCCCTGAATACTCACTCCTGGAATACGCTGCTGT‑AAACTGATTTTCATTCCATGaa                                                <  2:651334/239‑1 (MQ=255)
                                                                                                                                                                                         aaCTTCCTGGAACCACCGTTTATTGCAATCCGTCGCCGTAATTAACACCTTAACCTCAGGAAATTGCACCACGGTTTTTTGCAGCAACCAGTAGCAAAACTCACCATCCTGATCGCCATCGAGCATAACTAAGGCTTCAGGGTAACTTTCCAGCTTTTGCCATAACTCGTCTGCCTGACTGGCCCCCTGAATACTCACTCCTGGAATACGCTGCTGT‑AAACTGATTTTCATTCCATGaa                                                <  2:695232/239‑1 (MQ=255)
                                                                                                                                                                                          aCTTCCTTTAACCACCGTTTATTGCAATCCGTCGCCGTAATTAACCCCTTAACCTCAGGAAATTGCACCACGGTTTTTTGCAGCAACCAGTAGCAAAACTCACCATCCTGATCGCCATCGAGCATAACTAAGGCTTCAGGGTAACTTTCCAGCTTTTGCCATAACTCGTCTGCCTGACTGGCCCCCTGAATACTCACTCCTGGAATACGCTGCTGT‑AAACTGATTTTCATTCCATGaat                                               <  2:1132740/239‑1 (MQ=255)
                                                                                                                                                                                           cTTCCTGTAACCACCGTTTATTGCAATCCGTCGCCGTAATTAACACCTTAACCTCAGGAAATTGCACCACGGTTTTTTGCAGCAACCAGTAGCAAAACTCACCATCCTGATCGCCATCGAGCATAACTAAGGCTTCAGGGTAACTTTCCAGCTTTTGCCATAACTCGTCTGCCTGACTGGCCCCCTGAATACTCACTCCTGGAATACGCTGCTGT‑AAACTGATTTTCATTCCATGaata                                              >  2:402563/1‑239 (MQ=255)
                                                                                                                                                                                             tCCTGTAACCCCCGTTTATTGCAATCCGTCGCCGTAATTAACACCTTAACCTCAGGAAATTGCACCACGGTTTTTTGCAGCAACCAGTAGCAAAACTCACCATCCTGATCGCCATCGAGCATAACTAAGGCTTCAGGGTAACTTTCCAGCTTTTGCCATAACTCGTCTGCCTGACTGGCCCCCTGAATACTCACTCCTGTAATACGCTGCTGT‑AAACTGATTTTCATTCCATGaataaa                                            <  2:678136/239‑1 (MQ=255)
                                                                                                                                                                                              ccTGTAACCACCGTTTATTGCAATCCGTCGCCGTAATTAACACCTTAACCTCAGGAAATTGCACCACGGTTTTTTGCAGCAACCAGTAGCAAAACTCACCATCCTGATCGCCATCGAGCATAACTAAGGCTTCAGGGTAACTTTCCAGCTTTTGCCATAACTCGTCTGCCTGACTGGCCCCCTGAATACTCACTCCTGGAATACGCTGCTGT‑AAACTGATTTTCATTCCATGaataaat                                           >  2:791433/1‑239 (MQ=255)
                                                                                                                                                                                                 ggaaCCACCTGTTATTGCAATCCGTCGCCGTAATTAACACCTTAACCTCACGAAATTGCCCCACGGTCTTTTGCAGCAACCAGTAGCAAAACTCACCATCCTGATGGCCATCGAGCATAACGAAGGCTTCAGGGTAACTGTCCAGCTTTTGCCATAACTCGTCTGCCTGACTGGCCTCCTGAATACTCACTCCTGGAATACGCTGCTGT‑AAACTGATTTTCATTCCATGAATAAATAtt                                        <  2:974796/237‑1 (MQ=255)
                                                                                                                                                                                                       cccGTTTATTGCAATCCGTCGCCGTAATTAACACCTTAACCTCAGGAAATTGCACCACGGTTTTTTGCAGCAACCAGTAGCAAAACTCACCATCCTGATCGCCATCGAGCATAACTAAGGCTTCAGGGTAACTTTCCAGCTTTTGCCATAACTCGTCTGCCTGACTGGCCCCCTGAATACTCACTCCTGGAATACGCTGCTGT‑AAACTGATTTTCATTCCATGAATAAATATTGACTGc                                  <  2:1099651/238‑1 (MQ=255)
                                                                                                                                                                                                          gTTTATTGCAATCCGTCGCCGTAATTAACACCTTAACCTCAGGAAATTGCACCACGGTTTTTTGCAGCAACCAGTAGCAAAACTCACAATCCTGATCGCCATCGAGCATAACTAAGGCTTCAGGGTAACTTTCCAGCTGTTGCCATAACTCGTCTGCCTGACTGGCCACCTGAATACTCACTCCTGGAATAAGCTCCTGT‑AAACTGATTTTCATTCCATGAAAAAAAAATGaatcccag                               >  1:492925/1‑232 (MQ=255)
                                                                                                                                                                                                           tttAATGCAATCCGTCGCCGTAATTAACACCTTAACCTCAGGAAAGTGCACCACGGTTTTTTGCAGCAACCAGTAGCAAAACTCACCATCCTGATCGCCATCGAGCATAACTAAGGCTTCAGGGTAACTTTCCAGCTTTTGCCATAACTCGTCTGCCTGACTGGCCCCCTGAATACTCACTCCTGGAATACGCTGCTGT‑a                                                                     <  2:1254651/200‑1 (MQ=255)
                                                                                                                                                                                                            ttATTGCAATCCGTCGCCGTAATTAACACCTTAACCTAAGGAAATTGCACCACGGTTTTTTGCAGCAACCAGTAGCAAAACTCACCATCCTGATCGCAATCGAGCATAACTAAAGCTTCAGGGTAACTTTCCAGCTTTTGCCATAACTCGTCTGCCTGCCTGGCCCCCTGAATACTCACTCCTGGAATATGCGGCTGTGAAACTG‑TTTTCATTCCTTCAAAAAATATTAACTGCCTGTc                             >  1:868611/1‑239 (MQ=255)
                                                                                                                                                                                                             tATTGCAATCCGTCGCCGTAATTAACCCCTTAACCTCAGGAAATTGCACCACGGTTTTTTGCAGCAACCAGTAGCAAAACTCACCATCCTGATCGCCATCGAGCATAACTAAGGCTTCAGGGTAACTTTCCAGCTTTTGCCATAACTCGTCTGCCTGACTGGCCCCCTGAATACTCACTCCTGGAATACGCTGCTGT‑AAACTGATTTTCATTCCATGAATAAATATTGACTGCCTGTCa                            <  2:1018375/239‑1 (MQ=255)
                                                                                                                                                                                                                tGCAATCCGTCGCCGTAATTAACACCTTAACCTCAGGAAATTGCACCACGGTTTTTTGCAGCAACCAGTAGCAAAACTCACCATCCTGATCGCCATCGAGCATAACTAAGGCTTCAGGGTAACTTTCCAGCTTTTGCCATAACTCGTCTGCCTGACTGGCCCCCTGAATACTCACTCCTGGAATACGCTGCTGT‑AAACTGATTTTCATTCCATGaa                                                <  2:223842/216‑1 (MQ=255)
                                                                                                                                                                                                                   aaTCCGTCGCCGTAATTAACACCTTAACCTCAGGAAATTGCACCACGGTTTTTTGCAGCAACCAGTAGCAAAACTCACCATCCTGATCGCCATCGAGCATAACTAAGGCTTCAGGGTAACTTTCCAGCTTTTGCCATAACTCGTCTGCCTGACTGGCCCCCTGAATactca                                                                                           <  2:852675/171‑1 (MQ=255)
                                                                                                                                                                                                                   aaTCCGTCGCCGTAATTAACACCTTAACCTCAGGAAATTGCACCACGGTTTTTTGCAGCAACCAGTAGCAAAACTCACCATCCTGATCGCCATCGAGCATAACTAAGGCTTCAGGGTAACTTTCCAGCTTTTGCCATAACTCGTCTGCCTGACTGGCCCCCTGAATactca                                                                                           >  1:852675/1‑171 (MQ=255)
                                                                                                                                                                                                                     tCCGTCGCCGTAATTAACACCTTAACCTCAGGAAATTGCACCACGGTTTTTTgca                                                                                                                                                                                                             <  2:945134/55‑1 (MQ=255)
                                                                                                                                                                                                                     tCCGTCGCCGTAATTAACACCTTAACCTCAGGAAATTGCACCACGGTTTTTTgca                                                                                                                                                                                                             >  1:945134/1‑55 (MQ=255)
                                                                                                                                                                                                                     tCCGTCGCCGTAATTAACACCTTAACCTCAGGAAATTGCACCACGGTTTTTTGCAGCAACCAGTAGCAAAACTCACCATCCTGATCGCCATCGAGCATAACTAAGGCTTCAGGGTAACTTTCCAGCTTTTGCCATAACTCGTCTGCCTGACTGGCCCCCTGAATACTCACTCCTGGAATACGCTGCTGT‑AAACTGATTTTCATTcc                                                     <  2:835385/206‑1 (MQ=255)
                                                                                                                                                                                                                      ccGTTGCCGTAATTAACCACTTAACCACAGGAAATTGCGCCACGGGTTTTTGCAGCAAACATGAGCAAAACGCACCATCCTGATCTCCATCGAGCATAACTAAGGCTTCAGGGTAACTATCCAGCTTTTGCCATAACTCTGCTGCCTGACTGGCCCCCTGAATACTCACTCCTGGAATTTCCTGCTGT‑AAACTGATTTTCATTCCATGAATAAATATTGACTGCCTGTCAAACATGACt                   <  1:811070/239‑1 (MQ=255)
                                                                                                                                                                                                                      ccGTCCCCGTTATTAACCCCTTAACCCGAGGGAATTGCCCCCAGGTTTTTTGCAGCAACCAGTAGCAAAACTCCCCATCCTGATCGCCACCGAGCATAACTAATGCTTCAGGGTAACTTTCCAGCTTTTGCCATAACTCGTCTGCCTGACTGGCCCCCTGAATACTCACTCCTGGAATACGCTGCTGT‑AAACTGATTTTCATTCCATGAATAAATATTGACTGCCTGTCAAACATGACt                   <  1:392478/239‑1 (MQ=255)
                                                                                                                                                                                                                         tCGCCGTAATTAACACCTTAACCTCAGGAAATTGCACCACGGTTTTTTGCAGCAACCAGTAGCAAAACTCACCATCCTGATCGCCATCGAGCATAACTAAGGCTTCAGGGTAACTTTCCAGCTTTTGCCATAACTCGTCTGCCTGACTGGCCCCCTGAATACTCACTCCTGGAATACGCTGCTGT‑AAACTGATTTTCATTCCATGAATAAATATTGACTGCCGGTCAAACATGACTAtt                >  2:923454/1‑239 (MQ=255)
                                                                                                                                                                                                                          cGCCGTAATTAACACCTTAACCTCAGGAAATTGCACCACGGTTTTTTGCAGCAACCAGTAGCAAAACTCACCATCCTGCTCGCCATCGAGCATAACTAAGGCTTCAGGGTAACTTTCCAGCTTTTGCCATAACTCGTCTGCCTGACTGGCCCCCTGAATACTCACTCCTGGACTACGCTCCTGT‑AAATTGCTTTTCATTCCATGATTAAATATTTACTGCTTGCCAAACATgccacttt               >  1:1224374/1‑232 (MQ=255)
                                                                                                                                                                                                                          cGCCGTAATTAACACCTTAACCTCAGGAAATTGCACCACGGTTTTTTGCAGCAACCAGTAGCAAAACTCACCATCCTGATCGCCATCGAGCATAACTAAGGCTTCAGGGTAACTTTCCAGCTTTTGCCATAACTCGTCTGCCTGACTGGCCCCCTGAATACTCACTCCTGGAATACGCTGCTGT‑AAACTGATTTTCATTCCATGaa                                                <  2:167069/206‑1 (MQ=255)
                                                                                                                                                                                                                             ctttaTTACCCCCTTAACCCAACGAAATTGCCCCACGGTTTTTTGCAGCAACACATAGCAAAAATCACCATCCTGATCGCCATCGAGCATAACTAAGGCTTCAGGGTAACTTTCCAGCTTTTGCCATAACCCGTCTGCTTGACGGGCCCCCTGAATACTCACTCCTGGAATACGCTGCTGT‑AAACTGATTTTCATTCCATGAATAAATATTGACTGCCTGTCAAACATGACTATTTGCa            <  1:547976/235‑1 (MQ=255)
                                                                                                                                                                                                                             cttAATTAACCCCTTTACCACTGTAAATTTCCCCGCGGTTTTGTGGACCAACCGTTAGCAAAACCCCCCCCCCTGATCGCCATCGAGCATAAGTAAGGCTTCAGGGTAACGTTCCAGCTTTTGCCATAACTCGTCTGCCTGACTGGCCCCCTGAATACTCACTCCTGGAATACGCTGCTGT‑AAACTGATTTTCATTCCATGAATAAATATTGACTGCCTGTCAAACATGACTATTTGCa            <  1:962072/237‑1 (MQ=255)
                                                                                                                                                                                                                                 aTTAACACCTTAACCTCAGGAAATTGCACCACGGTTTTTTGCAGCAACCAGTAGCAAAACTCACCATCCTGATCGCCATCGAGCATAACTAAGGCTTCAGGGTAACTTTCCAGCTTTTGCCATAACTCGTCTGCCTGACTGGCCCCCTGAATACTCACTCCTGGAATACGCTGCTGT‑AAACTGATTTTCATTCCATGAATAAATATTGACTGCCTGTCAAACATGACTATTTGCATa          >  2:439623/1‑237 (MQ=255)
                                                                                                                                                                                                                                       acCTTAACCCCAGGAAATTGCCACACGGTTTTTTGCAGCAACCAGGAGCAAAACTCACCATCCGTATCGTCAACGAGCATAACTAAGGCTTCAGGGTAACTTTCCAGCTTTTGCCATAACTCGTCTGCCTGACTGGCCCCCTGAATACTCACTCCTGGAATACGCTGCTGT‑AAACTGATTTTCATTCCATGAATAAATATTGACTGCCTGTCAAACATGACTATTTGCATAACTGAAtc  <  2:567500/239‑1 (MQ=255)
                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                               
ACATGGTTAAAATCTCCCGCTGGCGGGCGCTTAACGATTTGAGATCTTTAATGTCCTTTTC‑CGGCGTCGTCCGCCAGTCTCCAGGCAGAAACATCATCCCCATCGCCGCA‑CTATTTACCGCCAACGCAAATGT‑CTCGACGGTTGAATCAC‑GAGGCACAATGGCCAGCACATTAAAATGGATAACTTCCTGTAACCACCGTTTATTGCAATCCGTCGCCGTAATTAACACCTTAACCTCAGGAAATTGCACCACGGTTTTTTGCAGCAACCAGTAGCAAAACTCACCATCCTGATCGCCATCGAGCATAACTAAGGCTTCAGGGTAACTTTCCAGCTTTTGCCATAACTCGTCTGCCTGACTGGCCCCCTGAATACTCACTCCTGGAATACGCTGCTGT‑AAACTGATTTTCATTCCATGAATAAATATTGACTGCCTGTCAAACATGACTATTTGCATAACTGAATC  >  NC_000913/3671437‑3671902

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A