Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F28 I0 R1 54 59.4 1320120 91.9% 1213190 218.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,065,603 T→A 29.8% I55F (ATC→TTC)  yihO ← putative sulphoquinovose importer

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,065,6030TA29.8% 66.4 / 7.8 45I55F (ATC→TTC) yihOputative sulphoquinovose importer
Reads supporting (aligned to +/- strand):  ref base T (24/7);  new base A (4/10);  total (28/17)
Fisher's exact test for biased strand distribution p-value = 2.88e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGT‑GG‑CGATAAACTGGAGCGTTGCAATTAA‑CGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGG‑TGAG‑CATATCGGTAAAC‑GCGGTAAAAAACTTCGCCACCAG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTTTATTATTATTACGCCGTGCGTTTACG  >  NC_000913/4065377‑4065825
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                              
tATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGT‑GG‑CGATAAACTGGAGCGTTGCAATTAA‑CGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAGTCCGG‑TGAG‑CATATCGGAAAAC‑GCGGAAAAAAACTTCGCCACCAG‑AAAAATa                                                                                                                                                                                                                               >  2:309267/1‑228 (MQ=255)
tATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGT‑GG‑CGATAAACTGGAGCGTTGCAATTAA‑CGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGG‑TGAG‑CATATCGGTAAAC‑GCGGTAAAAAACTTCGCCACCAG‑AAAGATa                                                                                                                                                                                                                               >  2:517299/1‑228 (MQ=255)
tATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGT‑GG‑CGATAAACTGGAGCGTTGCAATTAA‑CGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGG‑GGAG‑CATATCGGTAAAC‑GCGGTAAAAAACTTCGCCACCAG‑AAAGATa                                                                                                                                                                                                                               >  2:517502/1‑228 (MQ=255)
      aGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAACGGTGGT‑GG‑CGCTAAACTGGAGCGTTGCAATTAA‑CGCCGCCGGAACCGCTGCATATAAACTAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCAAGCAAAAATCCGG‑TGAG‑CAGATCGGAAAAC‑GCGGTAAAAAACTTGGCAACCAG‑AAAGATAATACCg                                                                                                                                                                                                                         >  2:583109/1‑228 (MQ=255)
      aGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAACGGTGGT‑GG‑CGATAAACTGGAGCGTTGCAATTAA‑CGCCGCCGGAACCGCTGCCTATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGTGTAAAAATCCGG‑TGAG‑CATATCGGTAAAC‑GTGGTAAAAACCTTCCACCACAG‑AAAGAAAAAccct                                                                                                                                                                                                                         >  1:140193/1‑227 (MQ=255)
        tCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGT‑GG‑CGATAAACTGGAGCGTTGCAATTAA‑CGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGG‑TGAG‑CATATCGGTAAAC‑GCGGTAAAAAACTTCGCCACCAG‑AAAGATAATCCCGcc                                                                                                                                                                                                                       >  2:13971/1‑228 (MQ=255)
          cAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGT‑GG‑CGATAAACTGGAGCGTTGCAATTAA‑CGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGG‑TGAG‑CATATCGGTAAAC‑GCGGGAAAAAACTTCGCCACCAG‑AAAGAAAATccctgtc                                                                                                                                                                                                                      >  2:146533/1‑223 (MQ=255)
          cAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGT‑GG‑CGATAAACTGGAGCGTTGCAATTAA‑CGCCGCCGGAACCGCTGCATATAAAATAAAGGGAAGAAATTTGCCTTTAGGCACAATATTTTTCCGAGATACGGGGAAAAATCCGGCTGA‑‑CATATCGGAAAAC‑GCGGTAAAAAACTTCACAAACAA‑AAAGAAAATCCCGccca                                                                                                                                                                                                                     >  1:347092/1‑226 (MQ=255)
                 catAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGT‑GG‑CGATAAACCGGAGCGTTGCAATTAA‑CGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGG‑TGAG‑CATATCGGTAAAC‑GCGGTAAAAAACTTCGCCACCAG‑AAAGATAATCCCGCCATAGAAAGc                                                                                                                                                                                                              >  2:67994/1‑228 (MQ=255)
                      acgcgggggggtgaaattggtttttAACCCCGAAACAAATGGGGGT‑GGTAGAAAAAACGGGGTGTTGAAATTAC‑CCCCGCCGGAACCGCTGCATAAAAAAAAAAGGGATGAAATTGGCCTTGAGGCCAAATTTTTTCCCGCGGGGCGAGAAAAAATCGGG‑TGAG‑AATACCGGTAAAC‑GCGGAAAAAACCTCCGCCCCCAG‑AAAGATAATCCCGCCATAGTAAGCAGGc                                                                                                                                                                                                          <  1:67994/207‑1 (MQ=255)
                          cgcgGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGT‑GG‑CGATAAACTGGAGCGTTGCAATTAA‑CGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCACGAGTCGAGTAAAAATCCGG‑TGGG‑CATATCGGTAAAC‑CCGGGAAAAACCTTCGCCAACAG‑AAAGAAAATCCCGCCAAAGAAAGAAGGCAAccc                                                                                                                                                                                                     >  1:362839/1‑228 (MQ=255)
                          cgcgGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGT‑GG‑CGATAAACTGGAGCGTTGCAATTAA‑CGCCGCCGGAACCGCTGCATATAAAAT‑AAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGG‑TGAG‑CATATCGGTAAAC‑GCGGTAAAAAACTTCGCCACCAG‑AAAGATAATCCCGCCATAGTAAGCAGGCATACCt                                                                                                                                                                                                    >  2:342996/1‑228 (MQ=255)
                                                aCCGGCAAACAAAAGGTGGG‑GG‑CGATAAACTGGAGCGTTGCAATTAA‑CGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGG‑TGAG‑CATATCGGTAAAC‑GCGGTAAAAAACTTCGCCACCAG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTg                                                                                                                                                                               >  2:486698/1‑228 (MQ=255)
                                                   cagaaaaaaaaGGGGGTGGG‑AAATAAAGGGGAGCG‑TGCAAATAACCGCCGCGGGCACCGTTCCATAAAAAAAAAAGGAAAGAATTTCCCCTTTGGCCACAATTTTTCCCCGAGGTTCGAGTAAAAACCGGG‑AGAT‑CTTACGGGAAAAC‑GCGGAAAAAAATTCCCCCACCAG‑AAAAAAAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTgag                                                                                                                                                                             <  1:202392/225‑1 (MQ=255)
                                                                   t‑gg‑CGATAAACTGGAGCGTTGCAATTAA‑CGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGCAATTTACCTTTAGGCCCAATATTTTTCCGCGAGTCGCGTAACAATCCGG‑TGCG‑CATATCGGCAAAG‑CCGGTAAAAACCTTCACCACCAA‑AAAGATAACCCCGCCACAGTAACCAGGCATCCCAACTTCCTCGCAATAAAATTTAGGGAGATCCA‑AGTAcacga                                                                                                                                                           >  1:379137/1‑224 (MQ=255)
                                                                     gg‑CGATAAACTGGAGCGTTGCAATTAA‑CGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGG‑TGAG‑CATATCGGTAAAC‑GCGGTAAAAAACTTCGCCACCAG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGAATAAAATTTGAGGAGGTACAAGGTACCCAt                                                                                                                                                           >  1:530861/1‑228 (MQ=255)
                                                                                        tGCAATTAA‑CGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGG‑TGAG‑CATATCGGTAAAC‑GCGGTAAAAAACTTCGCCACCAG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGCATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCg                                                                                                                                          >  2:433306/1‑227 (MQ=255)
                                                                                        tGCAATTAA‑CGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGG‑TGAG‑CATATCGGTAAAC‑GCGGTAAAAAACTTCGCCACCAG‑AAAGATAATCCCGCCATAGTAAGAAGGCATCCCTAATTCCTCGGTATAAAATTTGAGGAGATACACGGTACCGATGCAGCGAATTAAATTCg                                                                                                                                          >  1:502923/1‑227 (MQ=255)
                                                                                                                               aGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGG‑TGAG‑CATATCGGTAAAC‑GCGGTAAAAAACTTCGCCACCAG‑AAAGATAATCCCGCCATAGTCAGCAGGCATCCCTAATTCATCGGTATAAAAATTGAGGAGATACACAGTACCGTTGAAGCCCATTAAATTCGAACCGACGTCTCCCAAACCATAGGGGATTTTTTccccag                                                                                                   >  1:239209/1‑225 (MQ=255)
                                                                                                                               aGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGG‑TGAG‑CATATCGGTAAAC‑GCGGTAAAAAACTTCGCCACCAG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGACCCAACGTCGCCCATCCCATAGGCGATTTTTTCCCGCa                                                                                                   >  2:632113/1‑228 (MQ=255)
                                                                                                                               aGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGG‑TGAG‑CATATCGGTAAAC‑GCGGTAAAAAACTTCGCCACCAG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCcaca                                                                                                   >  1:444893/1‑228 (MQ=255)
                                                                                                                               aGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGG‑TGAG‑CATATCGGTAAAC‑GCGGTAAAAAACTTCGCCACCAG‑AAAGATAATCCCGCCATAGTAAGCAGGCACCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCa                                                                                                   >  2:176460/1‑228 (MQ=255)
                                                                                                                                   agaaaaTTCGCTTTGGGGCCAAATATTTCTCCGAGAGTAGAGAAAAAATCGGG‑TGAG‑CATATCGGAAAAC‑GCGGTAAAAAACTCCACCACCAA‑AAAGAAAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGAtt                                                                                               <  2:433280/225‑1 (MQ=255)
                                                                                                                                    cGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGG‑TGAG‑CATATCGGTAAAC‑GCGGTAAAAAACTTCGCCACCAG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTc                                                                                              >  2:463798/1‑228 (MQ=255)
                                                                                                                                                 tAGGCCTAATTTTTTGCCGGGGATCGAATAAAAAGAGGG‑TGAG‑CATGGCGGAAACC‑GGGGAAAAAAACCGCGCACCCAA‑AAAAATAACTCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCa                                                                                 <  1:286555/228‑1 (MQ=255)
                                                                                                                                                 tAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGG‑TGAG‑CATATCGGTAAAC‑GCGGTAAAAAACTTCGCCACCAG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTga                                                                                                                                                                              >  1:182510/1‑135 (MQ=255)
                                                                                                                                                 tAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGG‑TGAG‑CATATCGGTAAAC‑GCGGTAAAAAACTTCGCCACCAG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGAGGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGACa                                                                                 >  2:286555/1‑228 (MQ=255)
                                                                                                                                                 tAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGG‑TGAG‑CATATCGGTAAAC‑GCGGTAAAAAACTTCGCCACCAG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCAAAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCa                                                                                 >  2:502747/1‑228 (MQ=255)
                                                                                                                                                 tAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGG‑TGAG‑CATATCGGTAAAC‑GCGGTAAAAAACTTCGCCACCAG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGCATAAAATTTGAGGAGATACAGGGTACCGATGCAGAGCATTAAATTTGAACCGACGTCGCCCATACCAAAGGCGAATTTTTCCTCCAAATCCAGTTTTaacttc                                                                                  >  1:239974/1‑223 (MQ=255)
                                                                                                                                                 aaggcaaaaTGTTTTTCCGCGAGCTGA‑TAAAAAACCCG‑GGAGTCATCGCGGTAACC‑GGGGTAAAAATCTTCGCCACAAG‑AAGGAAAAACCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCa                                                                                 <  2:239209/221‑1 (MQ=255)
                                                                                                                                                  aGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGG‑TGAG‑CATATCGGTAAAC‑GCGGTAAAAAACTTCGCCACCAG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTgag                                                                                                                                                                             <  2:182510/135‑1 (MQ=255)
                                                                                                                                                   gcccaaaTTTTTTCCCGCAAACCGAAAAAAACCCCGG‑GGAG‑CATACCGTAAAGC‑GCGATAAAAAACCTCCCCACGAA‑AAAGAAAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGt                                                                               <  2:475893/223‑1 (MQ=255)
                                                                                                                                                        aattttttccggagaaccgaaaaaaacTCCGG‑TGAG‑AATACGGGTAAAC‑GGGGAAAAAAATTTCGCCACCAA‑AAAGAAAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGAtt                                                                          <  2:347092/206‑1 (MQ=255)
                                                                                                                                                             ttttCCGGAGGTCGAATAAAAATCGGG‑TGAG‑ATTACCGGAAAAC‑GGGGAAAAAAACTCCGCCCCAAA‑AAAGAAAACCCCGCCATAGTTAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGt                                                                     <  2:615454/228‑1 (MQ=255)
                                                                                                                                                             tttccccggattcaaatAAAAGTCCGG‑GGAC‑AATAGCGGAAAGC‑CCGGT‑AAAAACCTCCCCAACAGAAAAGATAACGCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGt                                                                     <  2:362839/212‑1 (MQ=255)
                                                                                                                                                                 cggtgcggctagaaaaacTCGGG‑TGAG‑TATATCGTTAACG‑GCAGAAAAAAACGTCGCCCACAA‑AAAAAAAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGa                                                                 <  1:13971/215‑1 (MQ=255)
                                                                                                                                                                         tgaatAAAACTCCGG‑TG‑G‑CCTTTCGGGAAACGGAGGAAAAAAACTCCGCCAACAG‑AAAAATATGCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCa                                                         <  1:159657/224‑1 (MQ=255)
                                                                                                                                                                                                      tAAAC‑GCGGTAAAAAACTTCGCCACCAG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCCGATTCAGTTTTAATGTCAGTGGATTTTGGTCACACATAATCACTCCTATTAACCCCCCTCAGGGggtgt                              >  1:150335/1‑226 (MQ=255)
                                                                                                                                                                                                      tAAAC‑GCGGTAAAAAACTTCGCCACCAG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGt                                                                     >  2:506046/1‑189 (MQ=255)
                                                                                                                                                                                                        aaC‑GCGGTAAAAAACTTCGCCACCAG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGCGGAGATACAGAGTACCGATGCAGAGCATTAACTTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGCGGATTATTGTCAGACATAATCACTCATTATAACGCCCCGCAGGGcgccgt                             >  2:384750/1‑227 (MQ=255)
                                                                                                                                                                                                            gcgtaaaaaaaCTCCGCCCGCAG‑AAAGAAAACCCCGACATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTttat                         <  2:502923/223‑1 (MQ=255)
                                                                                                                                                                                                            gcgATAAAAACTTTCCCCCCAAA‑AAAAAAAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTttat                         <  2:556112/228‑1 (MQ=255)
                                                                                                                                                                                                            gatgTAAAAAGCTCCGCCACAAA‑AAATAAAACCCCGCCATAGTAAAGAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATCCAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTttat                         <  1:486698/225‑1 (MQ=255)
                                                                                                                                                                                                                              acaaG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTTTATTATTATTACGCCGTGCGt       <  2:150335/225‑1 (MQ=255)
                                                                                                                                                                                                                                    aaaGATAACCCCGCCATAGTAAGGAGGCCTCCCTAATTCATCGGTATAACATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTTTATTATTATTACGCCGTGCGTTTACg  <  2:259094/228‑1 (MQ=255)
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                              
TATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGT‑GG‑CGATAAACTGGAGCGTTGCAATTAA‑CGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGG‑TGAG‑CATATCGGTAAAC‑GCGGTAAAAAACTTCGCCACCAG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTTTATTATTATTACGCCGTGCGTTTACG  >  NC_000913/4065377‑4065825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A