Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F17 I0 R1 52 59.5 1410130 88.0% 1240914 215.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,520,060 A→T 22.5% D345V (GAT→GTT)  curA → curcumin/dihydrocurcumin reductase, NADPH‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,520,0600AT22.5% 49.0 / 3.4 42D345V (GAT→GTT) curAcurcumin/dihydrocurcumin reductase, NADPH‑dependent
Reads supporting (aligned to +/- strand):  ref base A (12/16);  new base T (3/4);  total (22/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGAGATGGGGC‑AATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTA‑GAGAATGCGC‑CACAGACGTTTATCGGCCTGCTGAAGGGTAAAAA‑CTT‑CGGCAAAGTGGTGATCCGCGTGGCGGGTGATGATTAAA‑GTTTCAA‑CGGCGGC‑GTAAGCCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGCTTTAATTAACTAACCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGAGTGGTTTATGATTATTCACTTTAATACACCAGGTGAATTCCTTCTGCCATGCAGGCAGGGTTGGAC‑AGAAAACACGCGAAGGAATTAAAATGCCTGGAACGGG  >  NC_000913/1519838‑1520275
                                                                                                                                                                                                                                   |                                                                                                                                                                                                                           
cAGTGCTGCAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGAGATGGGGC‑AATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGGTTA‑GAGAATGCGC‑CACAGACGTTTATCGGCCTGCGGAAGGGGAAAAA‑CTT‑CGGCAAAGTGGAGATCCGCGTGGCGGGGGAGGATTaaa                                                                                                                                                                                                                        >  2:521049/1‑228 (MQ=255)
   tGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGAGATGGGGC‑AATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTA‑GAGAATGCGC‑CACAGACGTTTATCGGCCTGCTGAAGGGTAAAAA‑CTT‑CGGCAAAGTGGGGATCCGCGTGGCGGGGGCGGTTTAAA‑ggt                                                                                                                                                                                                                    >  2:302889/1‑226 (MQ=255)
   tGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGAGATGGGGC‑AATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTA‑GAGAATGCGC‑CACAGACGTTTATCGGCCTGCTGAAGGGTAAAAA‑CTT‑CGGCAAAATGGTGATCGGCGTGGAGGGGGATGATTAAA‑gat                                                                                                                                                                                                                    >  2:615135/1‑226 (MQ=255)
             aCGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTTAGAGGGAGATGGGGC‑AATGGGTGAAAGAGGATAAAATCAACTACCGCGAAGAAATTACTGACGGTTTA‑GAGAATGCGC‑CACAGACGTTTATCGGCCTGCTGAAGGGTAAAAA‑CTT‑CGGCAAAGTGGTGATCCGCGTGGCGGGTGATGATTAAA‑GTTTCAA‑cgccgg                                                                                                                                                                                                         >  2:97036/1‑228 (MQ=255)
                  ttCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGAGATGGGGC‑AATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTA‑GAGAATGCGC‑ACAAGGCGTTTATCGGCATGCTCGAGGGTAAAAA‑CTT‑CGGCAAACTGGTGATCCGCGTGGCGGGGGCAGGTTAAA‑AGTTCAA‑caagcgggggt                                                                                                                                                                                                    >  1:194842/1‑218 (MQ=255)
                         gCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGAGATGGGGC‑AATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTA‑GAGAATGCGC‑CACAGACGTTTATCGGCCTGCTGAAGGGTAAAAA‑CTT‑CGGCAAAGTGGTGATCCGCGTGGCGGGTGGTGATTAAA‑GTTTTAA‑AGGGGGC‑GTAAGACGCCa                                                                                                                                                                                            >  2:347670/1‑228 (MQ=255)
                                 ttattaTCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGAGATGGGGC‑AATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTA‑GAGAATGCGC‑CACAGACGTTTATCGGCCTGCTGAAGGGTAAAAA‑CTT‑CGGCAAAGTGGTGATCCGCGTGGCGGGGGATGGTTAAA‑GTTTCAA‑CGCGGGC‑GGAAACCGCCATaaaaaac                                                                                                                                                                                    >  2:656761/1‑226 (MQ=255)
                                         gCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGAGATGGGGC‑ATTGGGCGAAAGAGGATAAAATCCACTACCGCGAAGAAATTAATGACGGTTTA‑GAGAATGCGC‑CACAGACGTTTATCGGCCTGCTGAAGGGTAAAAA‑CTT‑CGGCAAAGTGGTGATCCGCGTGGCGGGTGATGATTAAA‑GTTTCAA‑AGGCGGG‑GTAAACCGGCATaaaaagaccgggaa                                                                                                                                                                             >  2:304876/1‑218 (MQ=255)
                                         gCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGAGATGGGGC‑AATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTA‑GAGAATGCGC‑CACAGACGTTTATCGGCCTGCTGAAGGGTAAAAA‑CTT‑CGGCACAGTGGAGATCAGCGGGGCGGGGGATGCGTAAA‑AATTTAA‑AGGCGGG‑GGAAGCGGCCCCaaaaaaaaagagaaa                                                                                                                                                                            >  1:368648/1‑218 (MQ=255)
                                                    tGGTCACCGCATCCATGAGTTTCAGAGGGAGATGGGGC‑AATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTA‑GAGAATGCGC‑CACAGACGTTTATCGGCCTGCTGAAGGGTAAAAA‑CTT‑CGGCAAAGTGGTGATCCGCGTGGCGGGTGATGTTTAAA‑GTTTCAA‑CGGCGGC‑GTAACGCGCCATAAAAATCCAGGCGAAAAGCTtttttt                                                                                                                                                                 >  2:12501/1‑228 (MQ=255)
                                                    tGGTCACCGCATCCATGAGTTTCAGAGGGAGATGGGGC‑AATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTA‑GAGAATGCGC‑CACAGACGTTTATCGGCCTGCTGAAGGGTAAAAA‑CTT‑CGGCAAAGTGGTGATCCGCGTGGCGGGGGATGATTAAA‑GTTTAAA‑AGGGGGG‑GTAAACCGCCCTAAAAATCCCAGCAAAAAACTTAtttt                                                                                                                                                                 >  2:472045/1‑228 (MQ=255)
                                                         aCCGCATCCATGAGTTTCAGAGGGAGATGGGGC‑AATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTA‑GAGAATGCGC‑CACAGACGTTTATCGGCCTGCTGAAGGGTAAAAA‑CTT‑CGGCAAAGTGGTGATCCGCGTGGCGGGGGATGTTTAAA‑GTTTCAA‑CGGCAGC‑GGAAGCCGCCATAAAAAGCCTAGTCACAAGGTTAtttttacct                                                                                                                                                            >  2:229592/1‑223 (MQ=255)
                                                         aCCGCATCCATGAGTTTCAGAGGGAGATGGGGC‑AATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTA‑GAGAATGCGC‑CACAGACGTTTATCGGCCTGCTGAAGGGTAAAAA‑CTT‑CGGCAAAGTGGTGATCCGCGTGGCGGGGGATGGTTAAA‑GTTTCCA‑CGGCGGCGGTAAACCGCCCAAAAAATCAGGGCAAAAACTTTTTTTTCAgt                                                                                                                                                             >  1:411308/1‑227 (MQ=255)
                                                         aCCGCATCCATGAGTTTCAGAGGGAGATGGGGC‑AATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTA‑GAGAATGCGC‑CACAGACGTTTATCGGCCTGCTGAAGGGTAAAAA‑CTT‑CGGCAAAGTGGGGATCGGCGTGGCGGGTGAGGGTTAAA‑GTTTAAA‑AGGCGGG‑GTAAGGCGCCCTAAAAATTCAGGCAAAAAACTTATTTTTAGCt                                                                                                                                                            >  2:252361/1‑228 (MQ=255)
                                                              aTCCATGAGTTTCAGAGGGAGATGGGGC‑AATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTA‑GAGAATGCGC‑CACAGACGTTTATCGGCCTGCTGAAGGGTAAAAA‑CTT‑CGGCAAAGTGGTGATCCGCGTGGCGGGGGGTGATTAAA‑GTTTCAA‑CGGCGGC‑GTAAACCGCCCTAAAAATCCACGCGAAAAGCTTATTTTCAGCTttaat                                                                                                                                                       >  2:351581/1‑228 (MQ=255)
                                                                    gggattCAGGGGGAAAAGGGGCAAAGGGGTAAAAGAGGAAAAAACCCCCTCCCGGGAAAAAATTCCGTACGGTTTA‑GAGATGGCCC‑CACAGCCGTTTATCGGCCTGCTGAAGGGTAAAAA‑CTC‑CGGCAAAGTGGTGACCCGCGTGGCGGGTGATGATTAAA‑GTTTCAC‑CGGCGGC‑GTAAGCCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGCTTTAATtaact                                                                                                                                                  <  2:344224/224‑1 (MQ=255)
                                                                       tttCAGAGGGAGATGGGGC‑AATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTA‑GAGAATGCGC‑CACAGACGTTTATCGGCCTGCTGAAGGGTAAAAA‑CTT‑CGGCAAAGTGGTGATCCGCGTGGCGGGTGATGATTAAAGGTTTCAA‑‑GGCGGC‑GGAAACCCGCACAAAAATCCACGACAAAAGTCTACATTCAACTTTCAATAAATAAcc                                                                                                                                              >  2:67376/1‑228 (MQ=255)
                                                                        ttgagggggAGAGGGGGC‑AGGGGGTGAAAGAGGAAAAAACCCCACGGCGAGAAGAAATGGCGGGCGGTTGA‑GATAGGGACC‑AACAGGCGTTTACGGGCCTGGGGAAGTAAAAAAA‑TTC‑CGGCAAATGGGTGCCCCGCGTGGGGGGTAAGGATTAAA‑GTTTCAA‑CGGCGGC‑TTAAGCCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGCTTTAATTAACTAACca                                                                                                                                             <  1:521049/222‑1 (MQ=255)
                                                                         tCAGAGGGAGATGGGGC‑AATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTA‑GAGAATGCGC‑CACAGAAGTTTATCGGCCTGCTGAAGGGTAAAAA‑CTT‑CGGCAAAGTGGTGATCAGCGAGGCGGGGGAGGGTTAAA‑GATTCAA‑CAGCGGC‑GTAAGGCCCCCTAAGAATCCAGGGAAAAAGATTAtttttatggtttattaacaaaacaa                                                                                                                                            >  1:266526/1‑207 (MQ=255)
                                                                                 agaTGGGGC‑AATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTA‑GAGAATGCGC‑CACAGACGTTTATCGGCCTGCTGAAGGGTAAAAA‑CTT‑CGGCAAAGTGGTGATCCGCGTGGCGGGGGAGGGTTAAA‑GTTTAAA‑CGGCGGG‑GGAAAGCGCCCAAAAAAACCCGGGGAAAAACTTATTTTTACGCttaattaaacaaacaaaaaaattt                                                                                                                                    >  1:57660/1‑212 (MQ=255)
                                                                                               ggTGAAAGGGGATAAATGCCACGACCGCAAAGAAATTCGTGACGGTTAA‑GGAAAGGCGC‑AACAGGCTTTTAAGGGCTGGTAGATGGATAAAAA‑CTG‑AGGAAAAGTGTTAATCGGCGTGGGGGTGGATGAGTAAA‑GTTGCAA‑CGGCGGC‑TTAAGCCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCATCTTTAATTAACTAACCACATCATTGTTCTGCTGCtaat                                                                                                                        <  2:320717/227‑1 (MQ=255)
                                                                                                             aaaCCCACTCCCGCGAAAAATTTTCGGAGGGTTTA‑AAGAAGGCGC‑CACAGGCGTTTATCGGCCTGGTGAAGGGTAAAAC‑CTT‑CGGCAAAGGGGTGACCCGCGTGGCGGTTTATGAAAAAA‑GTTTAAA‑CGGCGGC‑TTAAGCCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGCTTTAATTAACTAACCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGAg                                                                                                         <  1:550497/228‑1 (MQ=255)
                                                                                                              acccccccaCCGGGAAAAAATTTCTGACGGTTAA‑GAGAATCCGC‑CACGGACTTTTACGGGCCTGCGAAAGGATAAAAA‑CTT‑GGGCAAGGTGGTCACCCGCGTGGCGGGTGATGATTAAA‑GTTTCAA‑CGGCGGC‑ATAAGCCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGCTTTAATTAACTAACCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGAg                                                                                                         <  2:57660/219‑1 (MQ=255)
                                                                                                              aaTCCGCTACCGAAAAAAAATTATTG‑TGGTTTAGGAGATTCCAC‑GAGAGACGTTTACCGGCGTGCTGAAGTGTAAAAT‑CTA‑CGGCAATGTGGTGACCCGCGTGGCGGGTGATGATAAAA‑TTTTCAA‑CGGCGGC‑GTAAGCCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGCTTTAATTAACTAACCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGAg                                                                                                         <  1:387509/227‑1 (MQ=255)
                                                                                                                  cACTACCGAGAAAAAATTACTGACGGTTTA‑GGAAAGGCGC‑CACAGACGTTTATGGGCCTGGTAAAGGGTAAAAG‑CTT‑CGGAAAGGTGGTGATCCGGGTGGCGGGTGAGGATAAAA‑GTTTCAA‑CGGCGGC‑GTAAGCCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGCTTTAATTAACTAACCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGAGTGGtt                                                                                                    <  2:36057/228‑1 (MQ=255)
                                                                                                                          cgAAGAAATTACTGACGGTTTA‑GAGAATGCGC‑CACAGACGTTTATCGGCCTGCTGAAGGGTAAAAA‑CTT‑CGGCAAAGTGGTGATCCGCGTGGCGGGGGATGATTAAA‑GTTTCAA‑CGGCGGC‑GTAAGCCGCCCTAAAAATCCAGGGAAAAAAGTTATTTTTAGCTTTTATTAACTAACCACATCATTGTTTTGCATCTAATAAAACCAAAAGtatggggg                                                                                                     >  1:36057/1‑212 (MQ=255)
                                                                                                                            acgaatatACTGACGTTATA‑GAGAAGTGGA‑CCCAGTAGTTGAGCGACCGGCTGAAGGTTAAAAT‑CTG‑CGGGAAAGTGGTACTCCGCTGGGGGGGCGATGATTAAA‑TTTTAAA‑GAGCGGC‑ATAGACCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGCTTTAATTAACTAACCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGAGTGGTTTATGattatt                                                                                          <  2:672892/221‑1 (MQ=255)
                                                                                                                            aagaaTTTCCTGATGTTTTA‑AGGAAT‑CGCACACGGGCGTTTACCGGCCTGCTAAAGGGAAAAAA‑CTC‑AGAAAAAGTGGTATACCGCGTGGCGGGTGAAGATTAAA‑GTTTAAA‑CGGCGGC‑GTAAGCCGCCATAAAAATCCAGGCAAAAAGCTTAATTTCAGCTTTAATTAACTAACCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGAGTGGTTTATGattatt                                                                                          <  2:368648/228‑1 (MQ=255)
                                                                                                                                    aCTGACGTTTAA‑GAGAATGCGC‑CACGGACGTTTAGCGGCAGGTTGAAGTGTAAAAA‑CTT‑CGAAAAGGTGGTAATCCGCGTGGCGGGTAATGATTAAA‑GTTTCAC‑CGGCGGC‑GTAAGCCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGCTTTAATTAACTAACCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGAGTGGTTTATGATTATTCACTTTaa                                                                                  <  2:158553/228‑1 (MQ=255)
                                                                                                                                        aCGGTGTA‑GAGAAGGCGC‑CACAGACGTTTATGGGCGTGTTAAAGGGTAAAAT‑CTT‑CAGAAAAGTGGTGATCCGGGTGGCGGGTGATGATTAAA‑TTTTCAA‑CGGCGGC‑TAAAGCCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGCTTTAATTAACTAACCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGAGTGGTTTATGATTATTCACTTTAATaca                                                                              <  2:266526/228‑1 (MQ=255)
                                                                                                                                                 gaaaaTGCGC‑CACAGGCTTTAAGCGGCGTGGTAAAGGATAAAAA‑CTG‑CGGCAAGGTGGTGACCCGGGGGGCGGGGGAAGA‑TAAA‑ATTTCAAGCGGCGGC‑GAAAGCCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGCTTTAATTAACTAACCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGAGTGGTTTATGATTATTCACTTTAATACACCAGGTGa                                                                      <  2:564405/225‑1 (MQ=255)
                                                                                                                                                                 cggttttacgggcgtggtaaaggTAAAAA‑CTTGCGACAAAGTGGTGACCCGGGTGGGGGGAAAGGTTAAAA‑TTTACAA‑CGGGGCA‑GAAAGCCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGCTTTAATTAACTAACCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGAGTGGTTTATGATTATTCACTTTAATACACCAGGTGAATTCCTTCTGCCa                                                         <  2:294927/206‑1 (MQ=255)
                                                                                                                                                                  agagttaggggcaggcgAAAGGTAAAAAGCGT‑CGGCAAGTGGGTTCGCCGGGGGGGGGGGGATGTTTAAA‑TGTTCAC‑GGGCGAC‑GTAAGCCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGCTTTAATTAACTAACCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGAGTGGTTTATGATTATTCACTTTAATACACCAGGTGAAGTCCTTCTGCCATg                                                       <  2:590425/212‑1 (MQ=255)
                                                                                                                                                                     ttaccgtctggttgaatgtcaaaac‑CTG‑CGGCAAAGTGGTCAACGGCGTGGGTGGTAAGTTGTAAA‑TTTTCAA‑CGGGGTG‑CAAATCCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGCTTTAATTAACTAACCCCATCATTGTTCTGCTGCTAATAACAACAAAGGTGAGTGGTTTATGATTATTCACTTTAATACACCAGGTGAATTCCTTCTGCCATgcagg                                                   <  2:342320/208‑1 (MQ=255)
                                                                                                                                                                                               actgcgcaaaaGTGGTGATCCGAGGGGCGGGTATTGTTTAAA‑TTCTCAA‑CGGCGGG‑TAAATCCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGCTTTAATTAACTAACCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGAGTGGTTTATGATTATTCACTTTAATACACCAGGTGAATTCCTTCTGCCATGCAGGCAGGGTTGGAC‑AGAAAACACGCGa                          <  2:301026/220‑1 (MQ=255)
                                                                                                                                                                                                ttgcGGAAAAGGGGTGACCCGCGGGGCGGTGAATGATTAAG‑TTTTCAA‑GGGCGGC‑GAAAGCCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGCTTTAATTAACTAACCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGAGTGGTTTATGATTATTCACTTTAATACACCAGGTGAATTCCTTCTGCCATGCAGGCAGGGTTGGAC‑AGAAAACACGCGa                          <  2:352664/224‑1 (MQ=255)
                                                                                                                                                                                                taagaaacagggtgtgACCCGCTTGGCGGTTGATAATTAAA‑GTTCCAA‑GGGCGGC‑TAACGCCGCCATAAAAATCCAGGCAAAAAGCTTATTGTCAGCTTTAATTAACTAACCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGAGTGGTTTATGATTATTCACTTTAATACACCAGGTGAATTCCTTCTGCCATGCAGGCAGGGTTGGAC‑AGAAAACACGCGa                          <  2:581874/214‑1 (MQ=255)
                                                                                                                                                                                                   cGGCAAAGTGGTGATCCGCGTGGCGGGTGATGATTAAA‑GTTTCAA‑CGGCGGC‑GTAAGCCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGCTTTAATTAACTAACCACATCATTGTTTTGCTGCTAATAACAACAAAGGGGAGTGGTTTATGATTATTTACTTTAATAACCCAGGTGAATTCCTTTTGCCATGCAGGCAGGGGTGGAC‑AGAAAACACCCGAAg                        >  2:353137/1‑226 (MQ=255)
                                                                                                                                                                                                    ggCAAAGTGGTGATCCGCGTGGCGGGTGATGATTAAA‑GTTTCAA‑CGGCGGC‑GTAAGCCGCCCTAAAAATCCAGGGAAAAAAGTTATTTTTAGCTTTTATTTAACAACCACATCCTTTGTTTTCTGCCAATAAAAACCAAAGTGAGTGGGTTATGATTTTTTACTTTTATATACCAGGGGCATTCTTTTTGCCATGTATGTAGGGTTGGGC‑aaaaaaacaccgcaa                        >  1:411924/1‑216 (MQ=255)
                                                                                                                                                                                                           tggtgATCCGCGTGGCGGGTGATGATTAAA‑GTTTCAA‑CGGCGGC‑GTAAGCCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGCTTTAATTAACAAACCACATCATTGTTCTGCTGCTAATAAAAACAAAAGTGAGTGGGTTATTATTATTCACTTTAATACACCAGGGGAATTCCTTTTGCCATGCAGGCAGGGGTGGACAATAAAACAC‑CGAAGAAATTtaaaa              >  2:258757/1‑227 (MQ=255)
                                                                                                                                                                                                                        ggggggTAATAATTAAA‑GTTCAAG‑CGGGGGT‑GAAAGCCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGCTTTAATTAACTAACCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGAGTGGTTTATGATTATTCACTTTAATACACCAGGTGAATTCCTTCTGCCATGCAGGCAGGGTTGGAC‑AGAAAACACGCGAAGGAATTAAAATGCCTGGAACggg  <  2:411308/224‑1 (MQ=255)
                                                                                                                                                                                                                        ggCGGGTTATGATTAAA‑GTTACAA‑CGGGGGG‑GAAAGCCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGCTTTAATTAACTAACCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGAGTGGTTTATGATTATTCACTTTAATACACCAGGTGAATTCCTTCTGCCATGCAGGCAGGGTTGGAC‑AGAAAACACGCGAAGGAATTAAAATGCCTGGAACggg  <  2:411924/227‑1 (MQ=255)
                                                                                                                                                                                                                                   |                                                                                                                                                                                                                           
CAGTGCTGAAAAAACGTATTCGCTTGCAAGGTTTTATTATCGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGAGATGGGGC‑AATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTA‑GAGAATGCGC‑CACAGACGTTTATCGGCCTGCTGAAGGGTAAAAA‑CTT‑CGGCAAAGTGGTGATCCGCGTGGCGGGTGATGATTAAA‑GTTTCAA‑CGGCGGC‑GTAAGCCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGCTTTAATTAACTAACCACATCATTGTTCTGCTGCTAATAACAACAAAGGTGAGTGGTTTATGATTATTCACTTTAATACACCAGGTGAATTCCTTCTGCCATGCAGGCAGGGTTGGAC‑AGAAAACACGCGAAGGAATTAAAATGCCTGGAACGGG  >  NC_000913/1519838‑1520275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A