Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F27 I0 R1 322 127.1 2757226 91.8% 2531133 223.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,411,020 A→C 11.4% V310G (GTG→GGG)  ttcA ← tRNA s(2)C32 thioltransferase, iron sulfur cluster protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,411,0200AC11.4% 343.8 / 2.4 126V310G (GTG→GGG) ttcAtRNA s(2)C32 thioltransferase, iron sulfur cluster protein
Reads supporting (aligned to +/- strand):  ref base A (58/49);  new base C (4/14);  total (62/64)
Fisher's exact test for biased strand distribution p-value = 2.00e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 126 total aligned reads displayed.

TGTCGCGGTCCGTCAGGCTGTTGCTCATAAAGCATGGAAACAGTTACAGGGCGGGAAGATTAAAGGAAAAACGTGCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGT‑CTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGTTGGTATCGCACAGATGCGACGGCACCAC‑ATTCTGCATCGCGCTGAACATCGTCTCGATACGCCCTGGATAACGTTTATCCC  >  NC_000913/1410788‑1411257
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                              
tGTCGCGGTCCGTCAGGCTGTTGCTCATAAAGCATGGAAACAGTTACAGGGCGGGAAGATTAAAGGAAAAACGTGCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGT‑CTTATCCGGCCTCCATAAGAGTAGCCCGATACGCGTGCGC‑ACCGGGCGCTACCCTGGTTATTTTACTTCa                                                                                                                                                                                                                                           >  2:724583/1‑238 (MQ=255)
tGTCGCGGTCCGTAAGGCTGTTGCTCATAAAGCATGGAAACAGTTACAGGGCGGGAAGATTAAAGGAAAAACGTGCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGT‑CTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCACTTCaa                                                                                                                                                                                                                                          <  1:1166702/239‑1 (MQ=255)
       gTCCGTCAGGCTGTTGCTCATAAAGCATGGAAACAGTTACAGGGCGGGAAGATTAAAGGAAAAACGTGCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGT‑CTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCACTTCAACCCCAt                                                                                                                                                                                                                                    >  1:1364111/1‑238 (MQ=255)
       gTCCGTCAGGCTGTTGCTCATAAAGCATGGAAACAGTTACAGGGCGGGAAGATTAAAGGAAAAACGTGCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGT‑CTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTCGGC‑ATCGGGCGCTATCCTGGCTATTTACCTCCAAacacct                                                                                                                                                                                                                                    >  1:1039449/1‑236 (MQ=255)
        tCCGTCAGGCTTTTGCTCATAAAGCATGGAAACAGTTACAGGGCGGGAAGATTAAAGGAAAAACGTGCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGT‑CTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCACTTCAACCACAtt                                                                                                                                                                                                                                   <  1:718174/238‑1 (MQ=255)
          cGTCAGGCTGTTGCTCATAAAGCATGGAAACAGTTACAGGGCGGGAAGATTAAAGGAAAAACGTGCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGT‑CTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGC‑ATCGGGCGCTTTCCTGGTTATTTCCCTTCAACCACATTCa                                                                                                                                                                                                                                 >  1:8037/1‑238 (MQ=255)
                 cTGTTGCTCATAAAGCATGGAAACAGTTACAGGGCGGGAAGATTAAAGGAAAAACGTGCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGT‑CTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTa                                                                                                                                                                                                                           <  1:1019780/237‑1 (MQ=255)
                  tGTTGCTCATAAAGCATGGAAACAGTTACAGGGCGGGAAGATTAAAGGAAAAACGTGCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGT‑CTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAAc                                                                                                                                                                                                                         <  1:550696/238‑1 (MQ=255)
                    ttGCTCATAAAGCATGGAAACAGTTACAGGGCGGGAAGATTAAAGGAAAAACGTGCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGT‑CTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCa                                                                                                                                                                                                                      >  1:736298/1‑239 (MQ=255)
                        tCATAAAGGATAGAAACAGTTACAGGGCGGGAAGGTTAAAGGAAAAACGTGCCGGGTTCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGT‑CTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATcc                                                                                                                                                                                                                   <  1:456655/238‑1 (MQ=255)
                                 aTGGAAACAGTTACAGGGCGGGAAGATTAAAGGAAAAACGTGCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGT‑CTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGC‑ATCGGGCGCAATCCTGGTTATTTCACTTCAACCCAATTCAGCCGTAACTCATCCAACTGAttt                                                                                                                                                                                                          >  1:506613/1‑238 (MQ=255)
                                   ggAAACAGTTACAGGGCGGGAAGATTAAAGGAAAAACGTGCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGACGCGACGCTGGCCGCGT‑CTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCa                                                                                                                                                                                                       <  1:1197850/239‑1 (MQ=255)
                                          gTTACAGGGCGGGAAGATTAAAGGAAAAACGTGCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGT‑CTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGCTTTTCAtcttct                                                                                                                                                                                                 >  1:327014/1‑238 (MQ=255)
                                          gTTACAGGGCGGGAAGATTAAAGGAAAAACGTGCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGT‑CTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCAtcttctt                                                                                                                                                                                                >  1:92188/1‑239 (MQ=255)
                                           ttACAGGGCGGGAAGATTAAAGGAAAAACGTGCCGGGTGCGGTTATTAAAATACGGAAATGTTGAATTGACGGGCGCAAGAGTAAACATCTTATTCCGGATTGCCGGGTGCGACGCGGGCCGCGT‑CTTATCCGTCCTCCATAAGAGTAACCCGATACGCTTGCGC‑ATCGGTGGCTTTCCTGGTTTTTTCCCTTCCACCACCTTCACCCTTAAACCACCCACACGGTTTTCAtctttatt                                                                                                                                                                                               >  2:882288/1‑235 (MQ=255)
                                           ttACAGGGCGGGAAGATTAAAGGAAAAACGTGCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGT‑CTTATCCGACCTCCATAAGAGTAGCCCGCTACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACCGATTTTcgtattat                                                                                                                                                                                                 >  1:905580/1‑230 (MQ=255)
                                                   cGGGAAGATTAAAGGAAAAACGTGCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGT‑CTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCCCTTCAACCCCATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTTTGGGTGcc                                                                                                                                                                                       >  2:405127/1‑239 (MQ=255)
                                                     ggAAGATTAAAGGAAAAACGTGCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGT‑CTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTAACTTCAACCACATTCAGCCGTAACTCATCAAACTGATTTTAAACTTCTTTtgcctgcccg                                                                                                                                                                                     >  2:662310/1‑237 (MQ=255)
                                                     ggAAGATTAAAGGAAAAACGTGCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGT‑CTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGC‑ATCGGGCGCTATCCTGGGTATTTCACTTCAACCACATTCAACCGTAACTCATCCAAATGATTTTTATTTTTTTCTGGCTGCCa                                                                                                                                                                                      >  2:1340889/1‑238 (MQ=255)
                                                        aGATTAAAGGAAAAACGGTCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGT‑CTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCAc                                                                                                                                                                                  <  1:724583/239‑1 (MQ=255)
                                                           ttAAAGGAAAAACGTGCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGT‑CTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGC‑ATCGGGCGCTATCCTGGGTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTTTTTTGGCTGCCAGCACGcc                                                                                                                                                                               >  1:55687/1‑239 (MQ=255)
                                                             aaaGGAAAAACGTGCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGT‑CTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCgg                                                                                                                                                                             <  1:868009/239‑1 (MQ=255)
                                                             aaaGGAAAAACGGGCCGGGTGCGGTTATTAAAATAATGAAAGGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGT‑CTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCgg                                                                                                                                                                             <  1:763695/239‑1 (MQ=255)
                                                                          gcagggttgggTTAATAAAATAATGAAATGTTGAATTGCCGGGTGAAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGT‑CTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCACTTCAAACACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGAt                                                                                                                                                                 <  1:882288/229‑1 (MQ=255)
                                                                                tggcgTTATTAAAATTATGAAATGTTAAATTGCCGGGTACAAGAGTAACCATCTTATTCGGGATGGCCGGATGCGACGCTGGCCGCGG‑CTTAGCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTcg                                                                                                                                                          <  2:353759/235‑1 (MQ=255)
                                                                                tGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGT‑CTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGTTGCCAGCCCGCCGGTTGTAGTGGGACATCTTcg                                                                                                                                                          >  1:906046/1‑239 (MQ=255)
                                                                                  ggttttttaAAAAAATGAAATTTTTGATTGCCGGGGTCAAGGTTAAAAATTTTATTCGGGATTGCCGGATGGGACGCTGGCCGCGG‑TTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGTCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTcgc                                                                                                                                                         <  2:535014/232‑1 (MQ=255)
                                                                                      tattaAAATTAGGGAATGTTGAATTGGCGGGTGGCAGAGTAAAAGTCTTATTCGGGATTGCCGGATGCGCAGCTGGCCGCGT‑CTTATCCGGCCTTCATAAGAGTAGCCCGATACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCa                                                                                                                                                    <  1:617275/239‑1 (MQ=255)
                                                                                      tattaAAATAATGAAATTTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGT‑CTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCa                                                                                                                                                    <  2:736298/239‑1 (MQ=255)
                                                                                      tattaAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTAGTCGGGATTGCCGGATGCGACGCTGGCCGCGT‑CTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATc                                                                                                                                                     <  1:799885/238‑1 (MQ=255)
                                                                                      tattaAAAATATGAAATGTTGAATTGGCGGGTGCAAGGATAAACCTCTTATTCGGGATTGCCGGATGCGACGCTGGCCGGTT‑CTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCa                                                                                                                                                    <  2:60027/239‑1 (MQ=255)
                                                                                          aaaaTAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGT‑CTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTTTAGTGGGGTCCCTTTCCGTTCAAAc                                                                                                                                                 >  2:1060113/1‑238 (MQ=255)
                                                                                           aaaTAATTAAATTTTGAATTGCCGGGTGCAAGGGTAAACATCTTATTCGGGATGGCCGGATGGCACGCTGGCCGCGT‑CTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGc                                                                                                                                               <  2:57055/239‑1 (MQ=255)
                                                                                           aaaTAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGT‑CTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGc                                                                                                                                               <  1:467838/239‑1 (MQ=255)
                                                                                             ataatGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGT‑CTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCa                                                                                                                                             >  2:124095/1‑239 (MQ=255)
                                                                                                 tGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGT‑CTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCCCGATCAAACGCCAGATc                                                                                                                                         >  1:543248/1‑239 (MQ=255)
                                                                                                             ttGCCGGGTGCAAGGTTAAACATTTTATTCGGGGTGGGCGGATGCGCCGCGGGCCGCGT‑CTTAGCCGGCCGCCATAAGAGTAGCCCGATACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAAc                                                                                                                             <  2:1093530/239‑1 (MQ=255)
                                                                                                             ttGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGT‑CTTAGCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAAc                                                                                                                             <  1:1093676/239‑1 (MQ=255)
                                                                                                             ttGCCGGGTGCAAGAGTAAACATCTTAGTCGGGATTGACGGATGCGACGCTGGCCGCGT‑CTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGC‑ATCGGGCGCTATCCTGGTTAGTTCACGTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAAc                                                                                                                             <  1:1140895/239‑1 (MQ=255)
                                                                                                               cccGGGTGTACGAGTAAAATCCTGATTCGGGATCGCCGGTTGCGACGCTGGCCGCTT‑CTGATCCGGCCTCCGTAAGAGTAGCCCGATCCGCTTGCGC‑ATCGGGCGCTATCCGTGTTATTTCCCTTCAACCACCTTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCCCCCCCGTTAACCa                                                                                                                           <  2:1257143/238‑1 (MQ=255)
                                                                                                                ccGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGT‑CTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGCTCAAACGCCAGATCACCCCCGTTAAcc                                                                                                                            >  1:548700/1‑237 (MQ=255)
                                                                                                                ccGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGT‑CTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAAcc                                                                                                                            >  1:45042/1‑237 (MQ=255)
                                                                                                                         agatTAAAAATCTTATTGGGGATTGCCGGATGCGACCCTTGCCGCTT‑CTTGTCCGGCCTCGATAAGAGTCGCCCGATACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCAGAAcc                                                                                                                 <  2:445971/239‑1 (MQ=255)
                                                                                                                          aagTAAACAGCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGT‑CTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGC‑ATCGGGCGCTAGCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCg                                                                                                                <  1:204834/238‑1 (MQ=255)
                                                                                                                             tAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGT‑CTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTgg                                                                                                             >  1:1281416/1‑239 (MQ=255)
                                                                                                                               aaCATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGT‑CTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCTCGTTCAAACGCCAGATCACCCCCGTTAACCACTTCACAACCGTGGGt                                                                                                           >  1:1035452/1‑239 (MQ=255)
                                                                                                                                 cctttttttCGGGATGGGCGGATGCGACCCTGGCCGCGT‑CTTACCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGa                                                                                                         <  2:1364111/232‑1 (MQ=255)
                                                                                                                                  ctCTTATTCGGGATTGCCGGGTTCCACGTTGGCC‑CGTGTTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGAt                                                                                                        <  2:543248/238‑1 (MQ=255)
                                                                                                                                     ttATTCGGGATTGCCGGATGCGACGCTGGCCGCGT‑CTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCCCGATCAAACGCCAGATCACCCCCGTTAACCACTTCCGAACCGGGGGtagtg                                                                                                       >  2:778345/1‑233 (MQ=255)
                                                                                                                                       aTTCGGGATTGCCGGATGCGACGCTGGCCGCGT‑CTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCCCCCCCGTTAACCACTTCGGAACCCTGGGCCATGCCtt                                                                                                   >  1:52893/1‑239 (MQ=255)
                                                                                                                                           gggATTGCCGGATGCGACGCTGGCCGCGT‑CTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGaa                                                                                               <  1:405127/239‑1 (MQ=255)
                                                                                                                                            ggATTGCCGGATGCGACGCTGGCCGCGT‑CTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAATGGGATCTCTTCGCGATCAAACGCCCGATCACCCCCGTTAACCCCTTCACAACCGTGGGGGAAGCCTTTGaa                                                                                               >  2:701655/1‑238 (MQ=255)
                                                                                                                                                 tccGGGTGCGCCGCGGGCCGCGT‑CTTACCCGGCCTCCATAAGAGTAGCCCCATACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCCCTTCAACCCCATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGaa                                                                                         <  2:855089/238‑1 (MQ=255)
                                                                                                                                                 gcggggggCGTCGCGGCCCGCGT‑TTTATCCGGGCCACATAAGAGTACCCCGATACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGaa                                                                                         <  2:914769/232‑1 (MQ=255)
                                                                                                                                                 gCCGGGTGGGAGGTTGGCGGCGT‑TTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTGCCTTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGaa                                                                                         <  2:327014/239‑1 (MQ=255)
                                                                                                                                                 gCCGGGTGCCGCGCTGGCCGGGT‑CTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGaa                                                                                         <  2:8037/239‑1 (MQ=255)
                                                                                                                                                 gCCGGATGCGACGCTGGCCGCGT‑TTTATCCGGCCTGCATAAGAGTAGCCCGATACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTGCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGaa                                                                                         <  1:586641/239‑1 (MQ=255)
                                                                                                                                                       tgacgcGCTGGGCGCGT‑CTTTTCCGGGCTCCATAAGAGTATCCCGATCCGCTGTCGC‑ATGGGGCGATATCCTGGTTATTTCTCTTTAACCACATTCAGCCGTAACGTAGCCAACCGATGTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGt                                                                                    <  2:1035452/233‑1 (MQ=255)
                                                                                                                                                       tGCGACGCTGGCCGCGT‑CTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGaa                                                                                         <  1:647657/233‑1 (MQ=255)
                                                                                                                                                       tGCGACGCTGGCCGCGT‑CTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGtt                                                                                   >  1:866448/1‑239 (MQ=255)
                                                                                                                                                           acccTGGCCGGCT‑CTTTCCCGGCCTCCATAAGAGTAGCCCGATCCGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGTTGGTa                                                                               <  2:848800/236‑1 (MQ=255)
                                                                                                                                                            cGCTGGCCCTGT‑CTTACCCGGCCCCCTTAAGAGTAGCCCGATACGCTTGCGC‑ATCGGGCGCTTTCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGTTGGTa                                                                               <  2:1281416/238‑1 (MQ=255)
                                                                                                                                                                 gCCGCGT‑CTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTa                                                                                                                                                                        >  1:303622/1‑144 (MQ=255)
                                                                                                                                                                 gCCGCGT‑CTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTa                                                                                                                                                                        <  2:303622/144‑1 (MQ=255)
                                                                                                                                                                  gtttataaagaacaggCCTCCATAAGAGGTGCCCGATACGCTTGCGC‑ATCGGGCGCTTTCCTTGTTATTTCCCTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGTTGGTATCGca                                                                          <  2:472252/224‑1 (MQ=255)
                                                                                                                                                                            aTCCGGCCTCCATAAGGGTAGCCCG‑TACGGTTTGGCGATCGGGCTGATTCCTGGTTTTTTCCCTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGTTGGTATCGCACAGATGCGACg                                                               <  2:92188/239‑1 (MQ=255)
                                                                                                                                                                               cGGCCTACATAAGAGTAGCCCGATACGCTTGCCC‑ATCGGGCGCTATCCTGGTTGTTTCACTTCAACCACTTTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGTTGGTATCGCACAGATGCGACGGca                                                            <  1:1115382/239‑1 (MQ=255)
                                                                                                                                                                                aggtgtgtaaaaggagcagcGATACGCTTGCGC‑ATCGGGCGCTATGCGGGTTATTTCACTTCAACCAGTTTCAGCCGTAACTCATCCAACTGATTTTCATCTTCGTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGTTGGTATCGCACAGATGCGACGGca                                                            <  1:53018/219‑1 (MQ=255)
                                                                                                                                                                                  ccTCCATAAGAGTAGCCCGATACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGTTGGTATCGCACAGATGCGACGGcacc                                                          >  1:135012/1‑238 (MQ=255)
                                                                                                                                                                                    tcaaTAAGAGTAGCCCGTTACGTTTGCGT‑AACGGGGGCTTTCCTGGTTATTTCCCTTCAACCACATTAAACCGTAACTCATCCAACGGATTTGCATCTTCTTCTGGCTGCCAGCACGCCGGGTGTAGTGGGATCGCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGTTGGTATCGCACAGATGCGACGGCACcac‑a                                                      <  2:52893/236‑1 (MQ=255)
                                                                                                                                                                                           gagTGGCCCGTCTCGCTTGCGC‑CTCTGGCCTTATCCTGGTTATTTCCCTTCAACCACATTCAGCCGTAACTCTTCCAACCGATTTTCAACTTCTTCTGGCTGCCAGCCCGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGTTGGTATCGCACAGATGCGACGGCACCAC‑ATTCTGCa                                               <  2:1058022/239‑1 (MQ=255)
                                                                                                                                                                                            agTAGCCCGATACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGTTGGTATCGCACAGATGCGAc                                                                >  1:472252/1‑222 (MQ=255)
                                                                                                                                                                                             gTAGCCCGATTCGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGTTGGTATCGCACAGATGCGACGGCACCAC‑ATTCTGCa                                               <  1:1060113/237‑1 (MQ=255)
                                                                                                                                                                                              tAGCCCGATACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGGGATGCCTTTGAAATCGAACAGGTTGGTATCGCACAGATGCGCCGGCACCAC‑ATTCTGCATc                                             >  1:513192/1‑238 (MQ=255)
                                                                                                                                                                                               aGCCCGATACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCTTTGGTGATGCCTTTGAAATCGACCAGCTTGGTATCGCACAAATGCGACGGCCCCAC‑ATTCGGCATccc                                           >  2:137442/1‑237 (MQ=255)
                                                                                                                                                                                                gCCCGATACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCTAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGaa                                                                                         <  1:1327332/193‑1 (MQ=255)
                                                                                                                                                                                                gCCCGATACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCCCTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGTTGGTATCTCACAGATGCGACGGAACCAC‑ATTCTGCATcgc                                           >  1:871851/1‑238 (MQ=255)
                                                                                                                                                                                                gCCCGATACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCCGAACCGCGCGTTGTGCCTTTGAAATCCAAACAGATGGTATCGCACAGAGGCGACGGccccaaagtcgccaccgcg                                           >  2:1112542/1‑225 (MQ=255)
                                                                                                                                                                                                gCCCGATACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCCAAACCGTGGGTCATGCCTTTGAACTCGAACAGGTTGGGATAGCACAAGTGCCACGGCACCAC‑ATTCGGCATcgc                                           >  2:415253/1‑238 (MQ=255)
                                                                                                                                                                                                gCCCGATACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGc                                                                                                      >  1:444048/1‑180 (MQ=255)
                                                                                                                                                                                                gCCCGATACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGt                                                                                    >  1:868590/1‑198 (MQ=255)
                                                                                                                                                                                                gCCCGATACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGt                                                                                    <  2:868590/198‑1 (MQ=255)
                                                                                                                                                                                                gCCCGATACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGTTGGTATCGCACAGATGCGACGGCACCAC‑ATTCTGCATcgc                                           >  1:377657/1‑238 (MQ=255)
                                                                                                                                                                                                gCCCGATACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGTTGGTATCGCACAGATGCGACGGCACCAC‑ATTCTGCATcgc                                           >  1:1014006/1‑238 (MQ=255)
                                                                                                                                                                                                gCCCGATACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGTTGGTATCGCACAGATGCGACGGCACCAC‑ATTCTGCATcgc                                           >  1:1266892/1‑238 (MQ=255)
                                                                                                                                                                                                 cccGATACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGTTCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGCGATGCCTTTGAAATCGAACAGGTTGGAATCGCACAGATGCGACGG‑GCCACAATTCTGAATcgc                                           >  1:1088788/1‑237 (MQ=255)
                                                                                                                                                                                                     aTACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGTTGGTATCGCACAGATGCGACGGCACCAC‑ATTCTGCATCGCGCTGaa                                     >  1:1346000/1‑239 (MQ=255)
                                                                                                                                                                                                      tACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCTTGGGTGATGCCTTTGAAATCGAACAGGTTGGTATCGCACAGATGCGCCGGCAACAC‑ATTACGCATCGCGCTGaaa                                    >  2:783309/1‑238 (MQ=255)
                                                                                                                                                                                                                  aTCGGGCGCTATTCTTGTTATTTAACTGCAACGACAGTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTATTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGTTGGTATCGCACAGATGCGACGGCACCAC‑ATTCTGCATCGCGCTGAACATCGTCTCGAt                         <  1:308052/239‑1 (MQ=255)
                                                                                                                                                                                                                  aTCGGGCGCTATCCTGGTTATTTCACCTCAACCACATTCAGGCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGTTGGTATCGCACAGATGCGACGGCACCAC‑ATTCTGCATCGCGCTGAACATCGTCTCGAt                         <  1:824574/239‑1 (MQ=255)
                                                                                                                                                                                                                       gcgcTAACCTTGTTATTTCCCTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTGCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTGCGCGATCAAACGGCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGTTGGTATCGCACAGATGCGACGGCGCCAC‑ATTCTGCATCGCGCTGAACATCGTCTCGATACGc                     <  1:415253/238‑1 (MQ=255)
                                                                                                                                                                                                                        gggtATCCTTGTTATTTCCTTTACACCCCATTCAGCCGTAACTCATCCAACTGAGTTTCATCTTCTTCTGGATGCCAGCACGACGTTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGTTGATGCCTTTGAAATCGAACAGGTTGGTATCGCACAGATGCGACGGCACCAC‑ATTCTGCATCGCGCTGAACATCGTCTCGATACGcc                    <  1:1189990/235‑1 (MQ=255)
                                                                                                                                                                                                                            aTCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGCTTGGAATCGCACAGATGCGACGGCACCAC‑ATTCTGCATCGCGCTGGACACCGTCCCGAAACGCCCggg                >  2:824574/1‑238 (MQ=255)
                                                                                                                                                                                                                             tCCTGGTTTTTTCACTTCAACCCCATTCAGCCGTAACCCTTCCAACTGATTTTCATCTTTTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGTTGGTATCGCACAGATGCGACGGCACCAC‑ATTCTGCATCGCGCTGAACATCGTCTCGATACGCCCTGGAt              <  2:623654/239‑1 (MQ=255)
                                                                                                                                                                                                                              ccTGGTTTTTTCCCTTCCACCCCATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGAACTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGCTGGTATCGCACAGATGCGACGGCACCAC‑ATTCTGCATCGCGCTGAACATCGTCTCGATACGCCCTGGATa             <  2:548700/239‑1 (MQ=255)
                                                                                                                                                                                                                                   ttATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGTTGGTATCGCACAGATGCGACGGCACCAC‑ATTCTGCATCGCGCTGAACATCGTCTCGATACGCCCTGGATAACGtt        >  1:623654/1‑239 (MQ=255)
                                                                                                                                                                                                                                     aTTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGTTGGTATCGCACAGATGCGACGGCACCAC‑ATTCTGCATCGCGCTGAACATCGTCTCGATACGCCCTGGATAACGttt       >  1:1296016/1‑238 (MQ=255)
                                                                                                                                                                                                                                      attCCCTTCACCCCCATTCAGCCGTTACTCATCCAACTTATTTTCTTCTTCTTCAGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCGAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGTTGGTAGCGCACAGATGCGACGGCACCAC‑ATTCTGCATCGCGCTGAACATCGTCTCGATACGCCCTGGATAACGTTTAt     <  2:562931/238‑1 (MQ=255)
                                                                                                                                                                                                                                       ttCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGACCAGGTTGGTATCCCCCAGATGCGACGGCACCAC‑ATTCTGCATCCCCCTGAACATCGCCTCGAAACGCCCTGGAAAAAGTTTAt     >  2:1376080/1‑238 (MQ=255)
                                                                                                                                                                                                                                       ttCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGTTGGTATCGCACAGATGCGACGGCACCAC‑ATTCTGCATCGCGCTGAACa                                   <  2:860417/208‑1 (MQ=255)
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                              
TGTCGCGGTCCGTCAGGCTGTTGCTCATAAAGCATGGAAACAGTTACAGGGCGGGAAGATTAAAGGAAAAACGTGCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGT‑CTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGC‑ATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCAGAACCGTGGGTGATGCCTTTGAAATCGAACAGGTTGGTATCGCACAGATGCGACGGCACCAC‑ATTCTGCATCGCGCTGAACATCGTCTCGATACGCCCTGGATAACGTTTATCCC  >  NC_000913/1410788‑1411257

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A