Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F27 I0 R1	322	127.1	2757226	91.8%	2531133	223.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,455,846	A→C	13.4%	H228P (CAC→CCC)	paaC →	ring 1,2‑phenylacetyl‑CoA epoxidase subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,455,846	0	A	C	13.4%	186.7 / 3.9	85	H228P (CAC→CCC)	paaC	ring 1,2‑phenylacetyl‑CoA epoxidase subunit
Reads supporting (aligned to +/- strand): ref base A (34/36); new base C (3/11); total (37/48)
Fisher's exact test for biased strand distribution p-value = 8.00e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCAGGGCAAAAGATGCAGCAGGCCATCAACAAGTTGTGGCGTTTTA‑CCGCCGAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGC‑‑GCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCAC‑ATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGAC‑TGCATACC‑G‑AACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGG‑TCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCGGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAATGTGACACAGATGGGAGAAGGATGGGTGATCGGCTTTA  >  NC_000913/1455613‑1456081

aTCAGGGCAAAAGATGCAGCAGGCCATCAACAAGTTGTGGCGTTTTA‑CCGCCGAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGC‑‑GCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCAC‑ATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGAC‑TGCATACC‑G‑AAAACCTTg                                                                                                                                                                                                                                         >  1:68416/1‑239 (MQ=255)

 tCAGGGCAAAAGATGCAGCAGGCCATCAACAAGTTGTGGCGTTTTA‑CCGCCGAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGC‑‑GCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCAC‑ATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGAC‑TGCATACC‑G‑AACACCTTg                                                                                                                                                                                                                                         >  1:743849/1‑238 (MQ=255)

 tCAGGGCAAAAGATGCAGCAGGCCATCAACAAGTTGTGGCGTTTTA‑CCGCCGAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGC‑‑GCAGCGGGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCAC‑ATTGAACGTACCGCAAGGGAAGGCGGATCGCACGGGCGGGAAAAAAGAAA‑TGCAAACC‑C‑AAAACCATgg                                                                                                                                                                                                                                        >  2:1344082/1‑239 (MQ=255)

     ggCAAAAGATGCAGCAGGCCATCAACAAGTTGTGGCGTTTTA‑CCGCCGAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGC‑‑GCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCAC‑ATTGAACGTACCGCAAGAGCAGGCGTATGGCACTGGCGGGAAAAAAAGCC‑TACAAACC‑G‑AACAACTTGGAcc                                                                                                                                                                                                                                     >  1:976878/1‑238 (MQ=255)

            aaTGCAGCAGGCCTTCAACAAGTTGGGGCGTTTAA‑CCGGCGAACTGTTCGATGGCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGC‑‑GCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCAC‑ATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGAC‑TGCATACC‑G‑AACACCTTGGACCCATGCTgg                                                                                                                                                                                                                             <  1:428979/238‑1 (MQ=255)

              tGCAGCAGGCCATCAACAAGTTGTGGCGTTTTA‑CCGCCGAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGC‑‑GCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCAC‑ATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGAC‑TGCATACC‑G‑AACACCTTGGACCCATGCTgg                                                                                                                                                                                                                             <  1:1344082/237‑1 (MQ=255)

               tcacgagGCCATTAAAAAGTTGTGGCGTTTTA‑CCGGCGAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGC‑‑GCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCAC‑ATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGAC‑TGCATACC‑G‑AACACCTTGGACCCATGCTGGCAg                                                                                                                                                                                                                          <  1:535297/234‑1 (MQ=255)

               gcagcaGGCCATCAACAAGTTGTGGCGTTTTA‑CCGCCGAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGC‑‑GCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCAC‑ATTGAACGTACCGCAAGAGCAGCCGTATCGCACTGGCGGTAAAAAAAGAA‑TCCATACC‑G‑AAAACCTCGGAccccgcgtggcgg                                                                                                                                                                                                                          >  1:1090887/1‑229 (MQ=255)

               cggtcagGCCAACAAAATGTTGTGG‑GTTTTACCCGCCGAACTGTTCGATGGCGACGGGATTGATATGGCACTGAGTGAAGAGGGTATTGCGGGTGATCCACGCACTTTACGC‑‑GCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCAC‑ATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGAC‑TGCATACC‑G‑AACACCTTGGACCCATGCTGGCAg                                                                                                                                                                                                                          <  2:1246384/235‑1 (MQ=255)

                   agacagaTCAACAAGTTGGGGCGTTTTA‑CCGCCGAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGC‑‑GCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCAC‑ATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGAC‑TGCATACC‑G‑AACACCTTGGACCCATGCTGGCAGAAAt                                                                                                                                                                                                                      <  1:580758/233‑1 (MQ=255)

                      gcaaacaaaatgtggtggtgTTTTA‑CCGCCGAACTGTTCGATCCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGATCCACGCACTCTACGC‑‑GCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCAC‑ATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGAC‑TGCATACC‑G‑AACACCTTGGACCCATGCTGGCAGAAAt                                                                                                                                                                                                                      <  1:685600/222‑1 (MQ=255)

                      gacaaaagaatgtgggggCTTTTTA‑CGGCAGGACTGTCCGATGGAGTCGGGTTTGATTTTGCACTGAGGTAAGGGGGGATTGCGGTTGATCAACGTACTTTACGC‑‑GCAGCGGGGGAGGGCGAAGTTTTTGCCGGGATCAACGAAGCCAC‑ATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGAC‑TGCATCCC‑G‑AACACCTTGGGCCCATGCTGGCAGAAAGGc                                                                                                                                                                                                                    <  2:976878/222‑1 (MQ=255)

                      gCCATCAACAAGTTGTGGCGTTTTA‑CCGCCGAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGC‑‑GCAGAGTGGGAAGCCGAAGATTTTGCCGGGATCAACGAAGCCAC‑ATTGAACGTAACGCAAGAGCAGGCGTATCGCACTGCAGGTAAAAAAGGGCGTCCGAACC‑G‑‑ACACCCTGGACCCCTGccaggggcaatgca                                                                                                                                                                                                                   >  2:829494/1‑226 (MQ=255)

                       cccacaacagttggggggtttttC‑CCGCCGAACTTGTCGATGCCGACGAGGTTGATATTGCACTGAGTGAAGAGGGTTTTGCGGTTGAGCCACGCACGTTACGC‑‑GCAGCGTGGGAAGCCGAAGTTTTTGGCGGGATCAACGAAGCCAC‑ATGGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGAC‑TGCATACC‑G‑AACACCTTGGACCCATGCTGGCAGAAATGCAg                                                                                                                                                                                                                  <  2:989349/220‑1 (MQ=255)

                        cATCAACAAGTTGTGGCGTTTTA‑CCGCCGAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGC‑‑GCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCAC‑ATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGGC‑TGCCTACC‑G‑AACACCCTGGACCCCTGCTGGCAGAAAAGCAGt                                                                                                                                                                                                                 >  1:62849/1‑239 (MQ=255)

                            cacatgtttgggctgtttA‑CCGCCGAACTGTTCGATGCCTACGATATTGATATTGCACTGAGGGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGC‑‑GCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCAC‑ATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGAC‑TGCATACC‑G‑AACACCTTGGACCCATGCTGGCAGAAATGCAGTAtct                                                                                                                                                                                                             <  2:882512/224‑1 (MQ=255)

                            aaaaattgggggggTTTTA‑CCGCAGAACTGTTCAAAGCCGACCAGATTGCTATTGCGCTGAGTGAAGAGGGTATTGCGGTGGATCCACGCACTTTACGC‑‑GCAGAGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCAC‑ATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGAC‑TGCATACC‑G‑AACACCTTGGACCCATGCTGGCAGAAATGCAGTAtct                                                                                                                                                                                                             <  2:1188571/226‑1 (MQ=255)

                                  ttGGGGCTTTTTA‑CCGCCGAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGC‑‑GCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCAC‑ATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGAC‑TGCATACC‑G‑AACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAg                                                                                                                                                                                                         <  1:715528/237‑1 (MQ=255)

                                    gtgGCGTTTTA‑CCGCCGAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGC‑‑GCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCAC‑ATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAAGAC‑TGCAAACC‑A‑AAAACCCTGGACCCAAGCGGGCAaaaaaacaggaaccccccggc                                                                                                                                                                                                      >  2:580758/1‑221 (MQ=255)

                                    gtgGCGTTTTA‑CCGCCGAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGC‑‑GCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCAC‑ATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGGAAAAAAGGAC‑ACCAAACC‑G‑CACACCTCGGACCCACGCCGCCAGaaaaacacaatccccaacgg                                                                                                                                                                                                      >  2:626547/1‑221 (MQ=255)

                                                ccgccgAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGCGAGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCAC‑ATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGAC‑TGCATACC‑G‑AACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCg                                                                                                                                                                                            <  2:1159606/239‑1 (MQ=255)

                                                cccccgAACTGTTCGGGTGCGAGGAGATTGATATTGCACTGAGTGAAGGGGGTATTGCGGTTGACCCACGCCATTTACGC‑‑GCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCAC‑ATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGAC‑TGCATACC‑G‑AACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGG‑t                                                                                                                                                                                         <  2:743849/236‑1 (MQ=255)

                                                  ggcggaCTGTTGGGTGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGC‑‑GCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCAC‑ATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGAC‑TGCATACC‑G‑AACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGG‑TCa                                                                                                                                                                                       <  2:421153/234‑1 (MQ=255)

                                                            tCGATGCCGACGAGATGGATATTGCACTGAGTGAAGAGGGTATTGCGGTGGATCCACGCACTTTACGC‑‑GCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCAC‑ATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGAC‑TGCATACC‑G‑AACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGG‑TCAGCAATGGTaa                                                                                                                                                                             <  1:1085859/239‑1 (MQ=255)

                                                              taTGCCGAGGGGATTGTTATTGCACTGGGTGAGGAGGGTATTGCGGTTGATCCACGCACTTTACGC‑‑GCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCAC‑ATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGAC‑TGCATACC‑G‑AACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGG‑TCAGCAATGGTAACa                                                                                                                                                                           <  2:733135/238‑1 (MQ=255)

                                                              gttGCCGGCGAGTTTGGTTTTGCCCTGAGTGAAGGGGGTTTTGCGGTTGATCCACGCACTTTACGC‑‑GCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCAC‑ATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGAC‑TGCATACC‑G‑AACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGG‑TCAGCAATGGTAACa                                                                                                                                                                           <  2:1374887/237‑1 (MQ=255)

                                                              gATGCCGGCGGGTTTGTTTTTTCATTGAGTTAAGAGGGTATTGCGGTTGATCCACGCACTTTACGC‑‑GCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCAC‑ATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGAC‑TGCATACC‑G‑AACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGG‑TCAGCAATGGTAACa                                                                                                                                                                           <  2:529992/239‑1 (MQ=255)

                                                              gATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGC‑‑GCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCAC‑ATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGAC‑TGCATACC‑G‑AACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGG‑TCAGCAATGGTAACa                                                                                                                                                                           <  1:626547/239‑1 (MQ=255)

                                                                     tcgggttttgttttGCTCTGGTTGAAGGGGGTTTTGCTGTTGGTCCCCGAACTTTACCG‑‑CCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCAC‑ATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGAC‑TGCATACC‑G‑AACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGG‑TCAGCAATGGTAACAGAGgaga                                                                                                                                                                    <  2:428630/227‑1 (MQ=255)

                                                                     acgaGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGC‑‑GCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCAC‑ATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGAC‑TGCATACC‑G‑AACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGG‑TCAGGCATGGGAACAaaggaag                                                                                                                                                                    >  1:428630/1‑237 (MQ=255)

                                                                      cgaGATTTATATTGCACGGAGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGC‑‑GCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCAC‑ATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGAC‑TGCATTCC‑G‑AACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGG‑TCAGCAATGGTAACAGAGgaga                                                                                                                                                                    <  1:396584/238‑1 (MQ=255)

                                                                       gagaTTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGC‑‑GCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCAC‑ATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGAC‑TGCATACC‑G‑AACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGG‑TCAGCAATGGTAACAGAGGAGATg                                                                                                                                                                  <  1:1186464/239‑1 (MQ=255)

                                                                               tatTGCACTGGGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGC‑‑GCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCAC‑AGTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGAC‑TGCATACC‑G‑AACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGG‑TCAGCAATGGTAACAGAGGAGATGGGTATGc                                                                                                                                                           <  1:295536/238‑1 (MQ=255)

                                                                               tatTGCACTGAGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGC‑‑GCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCAC‑ATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGAC‑TGCATACC‑G‑AACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGG‑TCAGCaa                                                                                                                                                                                   <  1:1360325/214‑1 (MQ=255)

                                                                                    cACTGAGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGC‑‑GCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCAC‑ATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGAC‑TGCATACC‑G‑AACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGcc                                                                                                                                                                                              >  1:330413/1‑199 (MQ=255)

                                                                                    cACTGAGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGC‑‑GCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCAC‑ATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGAC‑CGCCATCC‑G‑ACAAACTTTGACCCATGCTGTACGAAATGCAGGATCCTCAACGGGTCTTTCCCGG‑TCCGCACTGGGAAAAAGGGAAATGGGGATGCAAcc                                                                                                                                                       >  2:295536/1‑236 (MQ=255)

                                                                                    cACTGAGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGC‑‑GCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCAC‑ATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGT‑AAAAAGGAC‑TGCATACC‑G‑AACACCTTGGACCCCAGCTGGGAGAAATGCAGATTTTCCCGCGGGTCTTGCCCGG‑TCAGCAATGGGAAAAGAGGGAATGGGGATGCAACg                                                                                                                                                       >  2:1180510/1‑236 (MQ=255)

                                                                                    cACTGAGGGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGC‑‑GCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCAC‑ATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGAC‑TGCATACC‑G‑AACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGcc                                                                                                                                                                                              <  2:330413/199‑1 (MQ=255)

                                                                                        gAGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGC‑‑GCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCAC‑ATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGAC‑TGCATACC‑G‑AACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGG‑TCAGCAATGGTACCACAGGAGATGGGTATGCAACGTCTgg                                                                                                                                                  >  1:348470/1‑238 (MQ=255)

                                                                                         aGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGC‑‑GCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCAC‑ATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGAC‑TGCATACC‑G‑AACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTACCCGG‑TCAGCAATTGTAACACAGGAACTGGGGTTGCAACGTcacgc                                                                                                                                                 >  1:1169904/1‑234 (MQ=255)

                                                                                         aGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGC‑‑GCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCAC‑ATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAAGGA‑TCGACACC‑A‑CACCCCTTGGGACCATGCTGGGCGAAATACAGAATCTCACCCCGGTCTTTCCCGGCTCA‑CAAAGGTAACAAGGGGAATGGGAAAGCAAAAACTGGcc                                                                                                                                                >  2:1091055/1‑239 (MQ=255)

                                                                                         aGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGC‑‑GCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCAC‑ATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGGAAAAAAGGAC‑TGCATACC‑G‑AACCCCTTTGACCCATGCTGGCAGAAATGCAGGATCTCCAGCGGGTCTTGCCCGG‑TCAGCAATGGTAACAGAGGAGATGGGGATGCACCGGCTGGc                                                                                                                                                 >  1:57741/1‑238 (MQ=255)

                                                                                            gAAGAGGGTATGGCGGTTTATCCACGCACTTTACGC‑‑GCAGCGTGGGAAGCCGAAGTTTTTGCCGGAATCAACGAAGCCAC‑ATTGAACGTACCGAAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGAC‑TGCATCCC‑G‑AACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGG‑TCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGccac                                                                                                                                              <  1:1339353/238‑1 (MQ=255)

                                                                                                   gTATTGCGGTTGATCCACGCACTTTACGC‑‑GCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCAC‑ATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGAC‑TGCATACC‑G‑AACACCTTGGACCCATGCTGGCAGAAATGCAGTATTTCCAGCGTGTCTTGCCCGG‑TCAGCAATGGTAACCGAGGGGATGGGGATGAAACGTCTGGCCACCATTGCa                                                                                                                                       >  1:1083939/1‑238 (MQ=255)

                                                                                                   gTATTGCGGTTGATCCACGCACTTTACGC‑‑GCAGCGTGGG‑AGCCGAAGTTTTTGCCGGGATCAACGAAGCCAC‑ATTGAACGTACCGCAAGAGCAGGCGTATTGCACTGGCGGTAAAAAAGGAC‑TGCATACC‑G‑AACACCTTTGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGGGTCTTGCCCGG‑TCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCAc                                                                                                                                      >  1:1106293/1‑238 (MQ=255)

                                                                                                     aTTGCGGTTGATCCACGCACTTTACGC‑‑GCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCAC‑ATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGAC‑TGCATACC‑G‑AACACCTTTGACCCATGCTGGCAGAAATGCAGTATCTCCAACGGGTCTTTCCCGG‑TCCGCAATGGTAACAAAGGGACTGGGGATGCAACGTCTGCCCCCCATTGcccc                                                                                                                                     >  1:719974/1‑238 (MQ=255)

                                                                                                            ttGATCCACGCACTTTACGC‑‑GCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCAC‑ATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGAC‑TGCCTACC‑G‑AACAACTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGG‑TCAGCACTCGTAAAAGAGGAGGTGGGGAATGAAAGACTGGCCACCCCAGAACCGCAACAgg                                                                                                                             >  2:542730/1‑239 (MQ=255)

                                                                                                             gtccccccGCCCTGTACGC‑‑GCGGGGGGGGAGGCCGAATTTTTTGCGGGAAACAACGAAGCCAC‑ATGGAACTGACCCAAAGAGCAGGCTTACCGCCCTGGGGGAAAAAAGGGGC‑TGCATACC‑G‑AACCCCTGGGACCAATGCTGGCAGAAATGCAGTATCCCCGGGGTGTCTTCCCCGG‑TCAGAAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATGGCACCGCCACAgg                                                                                                                             <  2:1188868/231‑1 (MQ=255)

                                                                                                                ccctcGCACTTTACGC‑‑GAAGGGGGGGAAACCGAAGTTTTTGCCGGGATAAACGAAGCCAC‑ATTGAACGTACCGAAAGGGCAGGCGTATCGCACTGGCGGTAAAAAAGGAC‑TGCATACC‑G‑ACCACCTGGGACCCATGCTAGCAGAAATGCAGTATCTCCAGCGGGTCTTGCCCGG‑TAAGCAATGATAAAAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCa                                                                                                                         <  1:405524/235‑1 (MQ=255)

                                                                                                                cccACGCACTTTACGC‑‑GCAGCGTGGAAAGCCGAAGTTTTTGCGGGGACAAACGAAGCCAC‑ATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGAAAAAAAGGAC‑TGCATCCC‑G‑AACCCCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGG‑TCAGCAATGGTAACAGAGGAGAGGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCa                                                                                                                         <  1:312225/238‑1 (MQ=255)

                                                                                                                 ccACGCACTTTACGC‑‑GCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCAC‑ATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGAC‑TGCATACC‑G‑AACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGG‑TCAGCAATGGTAACAGAGgaga                                                                                                                                                                    <  1:647595/195‑1 (MQ=255)

                                                                                                                           tACGC‑‑GCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCAC‑ATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGAC‑TGCATACC‑G‑AACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGG‑TCAGCAATGGTAACAGAGGGGATGGGTATGCAACGTCTGTCCCCCATTGCACCGCCACAAGTTcccgaggattggg                                                                                                              >  1:233216/1‑227 (MQ=255)

                                                                                                                           tACGC‑‑GCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCAC‑ATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGAC‑TGCACACC‑G‑AAACCCCTGGGCCCCTGCCGGCGGAAATTCCGATTTCCCACCGCGGCCTTCCCGG‑CCCGCAAAGGGACACGAGGGGATGGGTATGCCACGCTTGGCCCCCCTGTCACCGCCCCAAGTGCagaggaaaaggg                                                                                                              >  2:312225/1‑228 (MQ=255)

                                                                                                                                     gCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCAC‑ATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGAC‑TGCATACC‑G‑AACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGG‑TCa                                                                                                                                                                                       <  2:25318/158‑1 (MQ=255)

                                                                                                                                     gCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCAC‑ATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGAC‑TGCATACC‑G‑AACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGG‑TCa                                                                                                                                                                                       >  1:25318/1‑158 (MQ=255)

                                                                                                                                              gCCGAAGTTTTTGCCGGGATCAACGAAGCCAC‑ATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGAC‑TGCATACC‑G‑AACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGG‑TCAGCAATGGTAACAAAGGAGATGGGGATGCAACGTCTGGCCCCCATTGCACCGCCACAGGTTCATGAGGAATGGGGGCTACTCAGCCCGAt                                                                                              >  1:232289/1‑238 (MQ=255)

                                                                                                                                                cGAAGTTTTTGCCGGGATCAACGAAGCCAC‑ATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGAC‑TGCATACC‑G‑AACACCTTGGACCCCTGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGG‑TCAGCAATGGTAACAGAGGAGATTGGGTTGCAACGTCTGGCCACCATTGCACCGCCACAAGTTCATGGGATATGGGCGCTCCTCAGCCAGATcac                                                                                           >  1:193091/1‑237 (MQ=255)

                                                                                                                                                 gAAGTTTTTGCGGTGATCAACGAAGCCAA‑ATTGACCGTACCTCAAGAGCAGCCGTATCGACCTGGCGGTAAAAAAGGCC‑TGCATCCC‑G‑AACCCCTGGGACCCATGCGGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGG‑TCAGCAATGTTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATcccg                                                                                          <  1:1141214/239‑1 (MQ=255)

                                                                                                                                                        ttcccGGAATCGACGAA‑CCACAATTGAACGTCCCGCAAGAGCAGGCTTATGGCCTGGGCGGT‑AAAAAGGAC‑TGCATACC‑G‑AACCCCTTGGACCCATGCTGGAAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGG‑TCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCCCCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATcccggacccgga                                                                                  <  1:1180510/236‑1 (MQ=255)

                                                                                                                                                         ggccggactgagcGAAGCCAC‑ACTGACCGTACCGAAAGAGCAGGCGTCCCGCAGTGGGGGAAAAAAGGTAG‑TGCATACG‑G‑ACCACCTGGGGCCCGTGCGGGCAGAAACGGAGTATCTACAGTGTGTCTGGCCGGG‑TCAGAAAGGGTAACAGAGGACATGGGTATGCAACGTCTGGCCCCAATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATcccggacccgga                                                                                  <  2:285386/227‑1 (MQ=255)

                                                                                                                                                              ggACCAAGGACGCCAA‑ATAGAACGTACCGCAAGAGGAGGCGTAACAGTCTGGGGGAAAAAAGGCAC‑GGCATCCC‑G‑AACCTCTTGGCCCCATGCGGGAAAAAATGCGGTATCCCGAGCGTGTTTTGCCGGG‑TCAGCAAGGGTAACAGGGGAGATGGGTATGCAAGGTCTGCCCACCTTGGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCGGAGATcc                                                                             <  2:348470/239‑1 (MQ=255)

                                                                                                                                                               gaccAACGAAGCCAC‑ATTGAACGTACCGCAAGAGCAGGCGTATCGCACGGGCGGTAAAAAAGGAC‑TGCATACC‑G‑AACACCTGGGACCCATGCTGGCAAAAATGCAGTATCTCCAGCGTGTCTTGCCCGG‑TCAGCAAGGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCCCCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCGGAGATcc                                                                             <  1:1213989/235‑1 (MQ=255)

                                                                                                                                                                         gCCAC‑ATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAAGAC‑TGCATACC‑G‑AACACCTTGGACCCATGCTTGCAGAAATGCAGTATCTCCAGCGCGGCTTACCCCG‑ACAGCACTGGGAACAGAGGCGATGGGTATGGAACGTCTGGACCCCCTTGAACCGCCACAGGTTCATGAGATATGGGGGCTACAAAGCCAGATCCCGGACCCAGACAACCCGGGGCTgtaa                                                                  >  2:1047940/1‑236 (MQ=255)

                                                                                                                                                                             c‑aTTGAACGTACCGAAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGAC‑TGCATCCC‑G‑AACCCCTTGGACCCATGCTGGCAGAAAGGCAGTATCCCCAGCGGGTCTTGCCCGG‑TCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCca                                                                                                                                 <  1:154959/172‑1 (MQ=255)

                                                                                                                                                                                   aaCGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGAC‑TGCATACC‑G‑AACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGG‑TCAGCAATgg                                                                                                                                                                                >  1:760590/1‑120 (MQ=255)

                                                                                                                                                                                   aaCGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGAC‑TGCATACC‑G‑AACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGG‑TCAGCAATgg                                                                                                                                                                                <  2:760590/120‑1 (MQ=255)

                                                                                                                                                                                         ccGCAAGAGCAGGCGTGTCGCACTGGCGGTAAAAAAGGAC‑TGCATACC‑G‑AACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGG‑TCAGCAATGGGAACAGAGGAGATGAGTATGCAACGTCTGGCCACCATTGCACCGCCCCAGGTTCATGAGATATGGGCGCTACCCAGCCAGATCCCGGACCCGGAGATCCCGGTGCTGACCATTCCTGCTTTAgtc                                                   >  1:894279/1‑237 (MQ=255)

                                                                                                                                                                                         ccGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGAC‑TGCATACC‑G‑AACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGG‑TCAGCAATGGTAACAgaggag                                                                                                                                                                     <  2:1172251/125‑1 (MQ=255)

                                                                                                                                                                                         ccGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGAC‑TGCATAAC‑G‑AACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGG‑TCAGCAATGGGAACAgaggag                                                                                                                                                                     >  1:1172251/1‑125 (MQ=255)

                                                                                                                                                                                         ccGAAAGAGCAGGCTTATCGCATTGGGGGAAAAAAAGGTC‑TGCATCCC‑G‑AACCCCTTGGACCCATGCT‑GCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGG‑TCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCGGAGATCCCGGTGCTGACCATTACTGATTTAGGCa                                                  <  1:572744/239‑1 (MQ=255)

                                                                                                                                                                                          cGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGAC‑TGCATACC‑G‑AACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGG‑TCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCCGATCCCGGACCCGGAGATCCCGGGGCGGACCCTTACt                                                            >  1:1083316/1‑229 (MQ=255)

                                                                                                                                                                                           gaaagcgaaGGCGTATCGCA‑TGGGAGTAAAAAAAGAC‑CAGAGTCC‑GCAACACTTAGGACACATGCTGGAAAAACGGCATTATCACCAG‑GGGTCTTTCCCGGTTCAGAAAGGGTAGGGGAGGAGATGGTTAGCCAACGTGTGCCCACCTTGGAACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCGGAGATCCCGGTGCTGACCATTACTGATTTAGGCAt                                                 <  1:1047940/231‑1 (MQ=255)

                                                                                                                                                                                           gaaaGGGCGGGCGTTTAGCACGGGCGGTAAAAAGGGAC‑TGCATTCC‑G‑AACCCCTTGGACCCAGGCTGGCAGAAAGGCATTAGCTCCAGCTTGTCTTGCCCGG‑TCAGCAAGGGTAACAGAGGAGATGGTTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCGGAGATCCCGGTGCTGACCATTACTGATTTAGGCAt                                                 <  1:395784/237‑1 (MQ=255)

                                                                                                                                                                                                  cAGGCGTATCGCACTGGCGGTAAAAAAGGAC‑TGCATACC‑G‑AACACCTTGGACCCAAGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGG‑TCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCCTTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCGGGGATCCCGGGGCTGGCCATTACTGATTTAGGCATGGTGCGt                                          >  1:960998/1‑239 (MQ=255)

                                                                                                                                                                                                         agcgaaCTGGCGG‑AAAAAAGGGC‑CGCAACCCAG‑ACCCCCTTGGACCCATGCTGGCAGAAATGCGTTATCTCAGGCGTGTCTTGCCCGG‑GCAGCAAGGGTAAAAGAGGAGATGGGTATGCAACGTCTGGCCACAATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCGGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAATGTGa                                   <  1:1315342/234‑1 (MQ=255)

                                                                                                                                                                                                         aTCGCACTGGCGGTAAAAAAGGAC‑TGCATACC‑G‑AACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGG‑TCAGCAATGGTAACAGAGGAGATGGGGATGCAACGTCTGGCCACCATTGCACCGCCCCAGGTTCATGAGATATGGGCGCTACTCAGCCCGATCCCCGACCCGGGGATCCCGGTGCTGACCCTTACTGATTTAGGCAAGGTGCGGAAtgtg                                    >  1:908210/1‑238 (MQ=255)

                                                                                                                                                                                                          tCGCACTGGCGGTAAAAAAGGAC‑TGCATACC‑G‑AACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGG‑TCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGAACCGGAGATCCCGGTGCTGACCCTTACTGATTTAGGCATGGTCGGTAATGTGaa                                  >  1:633111/1‑238 (MQ=255)

                                                                                                                                                                                                           gcacatGGCGGTAAAAAAGGAC‑GGCATCCC‑G‑AACACCGTGGACCCATGCTGGCAGAAATGCAGTTTCTCCGGGGTGTCTTGCCGGG‑TCAGAAATGGTAACAGAGGAGATGGTTATGCACCGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCGGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAATGTGaca                                 <  1:1091055/234‑1 (MQ=255)

                                                                                                                                                                                                           cgcctggggggaaaaaagGGAC‑TGCATACC‑G‑AACCCCTGGGACCTATGCTGGAAGAAATGCAGTTTCTCCAGCGTGTCTTCCCGGG‑TCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCCCCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCGGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAATGTGaca                                 <  1:529724/227‑1 (MQ=255)

                                                                                                                                                                                                               tggggtgTAAAAAAGGAC‑TGCATACG‑G‑AACGCCTTGGACCCATGCTGGAAGAAAGGCATGTCCGCAAGCGTGTCCTGCCGGT‑ACAGCAAGGGTAACAGAGGAAAGGGGTATGACACGCCGGCCCACCATTGCCCCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCGGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAATGTGACACAGa                             <  2:233216/233‑1 (MQ=255)

                                                                                                                                                                                                                  gCGGTAAAAAAGGAC‑TGCATACC‑G‑AACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGG‑TCAGCAATGGTAACAGAGGAGATGGGGATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCGGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGGCCGTAATGGGACACAAATgg                          >  1:1200520/1‑239 (MQ=255)

                                                                                                                                                                                                                                   gCATCCC‑G‑AACCCCTTGGACCCATGCTGGAAGAAATGCAGTATCGCCAGCGTGTCTTGCCCGG‑TCAGAATTGGAAACAGAGGAGATGGGTAGGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCGGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAATGTGACACAGATGGGAGAAGGATGGGTGAt          <  1:752886/239‑1 (MQ=255)

                                                                                                                                                                                                                                    cATACC‑G‑AACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGG‑TCAGCAAt                                                                                                                                                                                  <  2:334279/70‑1 (MQ=255)

                                                                                                                                                                                                                                    cATACC‑G‑AACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGG‑TCAGCAAt                                                                                                                                                                                  >  1:334279/1‑70 (MQ=255)

                                                                                                                                                                                                                                           g‑AACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGG‑TCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCGGGGATCCCGGTGCTGACCATTACTGATTTAGGAATGGTGCGTAATGGGACCCAGAGGGGAGAAGGATGGGGGATCGGCttt   >  2:250590/1‑239 (MQ=255)

                                                                                                                                                                                                                                              acacCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGG‑TCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCAAGATCCCGGACCCGGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGATGCGAAATGTGACACAGCTGGGAGAAGGATGGGGGATCGGCTTTa  >  1:789660/1‑238 (MQ=255)

                                                                                                                                                                                                                                                acCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGG‑TCa                                                                                                                                                                                       <  1:642886/55‑1 (MQ=255)

                                                                                                                                                                                                                                                acCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGG‑TCa                                                                                                                                                                                       >  2:642886/1‑55 (MQ=255)

ATCAGGGCAAAAGATGCAGCAGGCCATCAACAAGTTGTGGCGTTTTA‑CCGCCGAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGC‑‑GCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCAC‑ATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGAC‑TGCATACC‑G‑AACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGG‑TCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCGGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAATGTGACACAGATGGGAGAAGGATGGGTGATCGGCTTTA  >  NC_000913/1455613‑1456081

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A