Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F27 I0 R1 322 127.1 2757226 91.8% 2531133 223.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,322,973 A→G 12.7% pseudogene (259/426 nt) phnE ← defective phosphonate ABC transporter permease,transport, Transport of small molecules: Anions, membrane channel protein component of Pn transporter, membrane component of ABC superfamily

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,322,9730AG12.7% 189.5 / 2.4 82pseudogene (259/426 nt)phnEdefective phosphonate ABC transporter permease,transport, Transport of small molecules: Anions, membrane channel protein component of Pn transporter, membrane component of ABC superfamily
Reads supporting (aligned to +/- strand):  ref base A (45/25);  new base G (6/6);  total (51/31)
Fisher's exact test for biased strand distribution p-value = 3.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAACAGCGCCAG‑CACGCCAGCACGCCAGCGAACGGGCCGAGGCCGAC‑GGCGACCACGAACAGCATGGCG‑AAGACCATTT‑CGTTAATGGC‑GCGGCAGGCGTCCATCAGGCGGCGAACGGGCTGGTAAACCCACCACGGCACCAGGTTTTC‑GGCGCTCATCAGGCCAAAGGGGATGGAGAG‑AA‑CCACCGCCAGCGCGGTGCCCCAGACGGCGATTTGCAGCGTGACGGCCATTTCGGTGAGGTAATCCTGCCACTGGCTGAAATCGGGCGGGAAGAAGTC‑GGCGGCGAACGTCGCCATGTTGCCGCCGTCTTTGATCAGCGTAAGCGGGGCCATTTCCGCGCCCTGCCACGAGACGACCAACACGGCGAGTACAACGGCCCAGCTCAGAAGCGAGAACCAGCTGCGCTTGGG  >  NC_000913/4322739‑4323207
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                             
ggcTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAACAGCGCCAG‑CACGCCAGCACGCCAGCGAACGGGCCGAGGCCGAC‑GGCGACCACGAACAGCATGGCG‑AAGACCATTT‑CGTTAATGGC‑GCGGCAGGGGGCCCTCAGGCGGCGAACGGGCGGGTAAACCCCCCCCCGCCCCCGGTTTTC‑CGCGCCCCACAGGCCCAAGGGGGGGgagag‑a                                                                                                                                                                                                                                          >  2:947056/1‑239 (MQ=255)
 gcccAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAACAGCGCCAG‑CACGCCAGCACGCCAGCGAACGGGCCGAGGCCGAC‑GGCGACCACGAACAGCATGGCG‑AAGACCATTT‑CGTTAATGGC‑GCGGCAGGCGTCCATCAGGCGGCGAACGGGCTGGTAAACCCACCACGGCACCAGGTTTTC‑GGCGCTCATCAGGCCAAAGGGGATGgaga                                                                                                                                                                                                                                             <  1:1247742/233‑1 (MQ=255)
           ttCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAACAGCGCCAG‑CACGCCAGCACGCCAGCGAACGGGCCGAGGCCGAC‑GGCGACCACGAACAGCATGGCG‑AAGACCATTT‑CGTTAATGGC‑GCGGCAGGCGTCCATCAGGCGGCGAACGGGCTGGTAAACCCACCACGGCCCCAGGGTTTC‑AGGGCTCCTCCAGCCAAAGGGGAGGGGGGA‑AA‑CCACcccccc                                                                                                                                                                                                                              >  2:1247742/1‑237 (MQ=255)
            tCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAACAGCGCCAG‑CACGCCAGCACGCCAGCGAACGGGCCGAGGCCGAC‑GGCGACCACGAACAGCATGGCG‑AAGACCATTT‑CGTTAATGGC‑GCGGCAGGGGTCCATCAGGCGGCGAACGGGCCGGTAAACCCACCACGCCGCCAGGTTTTC‑GGGCCCCACCAGGCCAAAGGGGAGGGGGAG‑AC‑CACCCGCCaa                                                                                                                                                                                                                              >  1:651953/1‑237 (MQ=255)
               cccggccgggAAAGGAGCGTGGGGACCGGGCCGGTGGGGGGGATAAACCGGCCCAC‑ACCCCCACCCGGCCGGGGAAGGGGCGGGGGCCGAC‑GGCGACCACGAACAGCATGGCG‑AAGACCTTTT‑GGTTAGTGGC‑GGGGCAGGCGTCCATCAGGCGGGGAACGGGGGGGTAAACCCACCACGGCACCAGGTTTTC‑GGCGCTCATCAGGCCAAAGGGGATGGAGAG‑AA‑CCACCGCCAGcgcg                                                                                                                                                                                                                          <  2:1030532/231‑1 (MQ=255)
                     tCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAACAGCGCCAG‑CACGCCAGCACGCCAGCGCACGGGCCGAGGCCGAC‑GGCGACCACGAACAGCCTGGCG‑AAGACCCTTT‑CGTTAATGGC‑GCGGCCGGCGGCCATCAGGCGGCGCCCGGGCTGGGAAACCCACCACGGCCCCACGTTTTC‑GCGCCCCCTCCGGCCAACGGGGGGGAAGAG‑AA‑CCCCCGCCAGCGCGCCGccc                                                                                                                                                                                                                    >  2:7296/1‑239 (MQ=255)
                     tCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAACAGCGCCAG‑CACGCCAGCACGCCAGCGAACGGGCCGAGGCCGAC‑GGCGACCACGAACAGCATGGCG‑AAGACCATTT‑CGTTAATGGC‑CCGGCAGGCGTCCACCAGGCGGCGCACCGGCTGGGAAAACCACCACGGCCCCAGGTTTTC‑GGCGCTCACCAGGCCAAAAGGGATGGGGAG‑AA‑CAACCGCCACCGGGGGGcc                                                                                                                                                                                                                     >  2:1247922/1‑238 (MQ=255)
                             ccgcTTGGGGAGCCCGCCGGTGGGGTGGAGAACCCG‑GCCAGCCGCGCTATCGCCCTAGTGAACGGGCGGAGGCCGAG‑GGCGCCCCTGAACAGCA‑GGCGAAACACCATTT‑CGTAAATGGC‑GCGGCAGGCGTCCATCAGGCGGCGAACGGGTTGGGAAACCCCCCACGGCACCAGGTTTTC‑GGCGCTCATCAGGCCAAAGGGGATGGAGAG‑AA‑CCACCGCCAGCGCGGTGCCCCAGACg                                                                                                                                                                                                              <  2:596781/235‑1 (MQ=255)
                                   ggAGAGCACGCCGGTGGTGTGGATAAACAGCGCCAG‑CACGCCAGCACGCCAGCGAACGGGCCGAGGCCGAC‑GGCGACCACGAACAGCATGGCG‑AAGACCATTT‑CGTTAATGGC‑GCGGCAGGCGTCCATCAGGCGGCGACCGGGCTGGTAAACCCACCACCGCACCAGGGTTTC‑GGCGCTCATCAGGCCAAAGGGGATGGAGAG‑AA‑CCACCGCCAGCGGGGGGCCCCAGACGGCggcatg                                                                                                                                                                                                      >  2:1285371/1‑234 (MQ=255)
                                   ggAGAGCACGCCGGTGGTGTGGATAAACAGCGCCAG‑CACGCCAGCACGCCAGCGAACGGGCCGAGGCCGAC‑GGCGACCACGAACAGCATGGCG‑AAGACCATTT‑CGTTAATGGC‑GCGGCAGGCGTCCATCAGGCGGCGAACGGGCTGGTAAACCCACCACGGCCCCAGGTTTTC‑GGCGCTCATCAGGCCAAAAGGGGTGGAGAG‑AA‑CCACCGCCAGCCCGGTGCCCCCGACGGCGGtttt                                                                                                                                                                                                      >  1:863256/1‑238 (MQ=255)
                                      ggccacggggtgggtgTGGACAACGCGCCCCAG‑CACGACAGCCCGGCAGGAGACGGGCCGAGGCGGGC‑GGCGCACGAGAACAGCATGGCG‑AAGCCCATTT‑CGTTAAGGGC‑GCGGCGGGCGTCCACGAGGCGGCAAACGGGCTGGTAAACCCCCCACGGCACCAGGTTTGC‑GGCGCTCATCAGGCCAAAGGGGAGGGAGAG‑AA‑CCACCGCCAGCGCGGTGCCCCAGACGGCGATTTGCa                                                                                                                                                                                                    <  2:474863/226‑1 (MQ=255)
                                          acggggggggtgTGGATAAACCGCGCCCG‑CCCGCACGACCGCCAGCAAAGGGGCCGAGGCCGAG‑GGGGACCACGAGAAGATGGGCG‑ATG‑CCATTTGCGTAAGTGGC‑GGGGCAGGGGTCCATCAGGCGCGGAACGGGCTGGTAAACCCACCACGGCACCAGGTTTTC‑GGCGCGCATCAGGCCAAAGGGGATGGAGAG‑AA‑CCACCGCCAGCGCGGTGCCCCAGACGGCGATTTGCAGCGTg                                                                                                                                                                                               <  2:233815/231‑1 (MQ=255)
                                              cGGTGGTGTGGATAAACAGCGCCAG‑CACGCCAGCACGCCAGCGAACGGGCCGAGGCCGAC‑GGCGACCACGAACAGCATGGCG‑AAGACCATTT‑CGTTAATGGC‑GCGGCAGGCGTCCCTCAGGCGGCGAACGGGCTGGTAAACCCACCACGGCACCAGGTTTTC‑CGCGCTCATCAGGCCCAAAGGGATGGAGAG‑AA‑CCACCGCCAGCGCGGTGACCCCGAACGCGATTTGCAGGCTGACg                                                                                                                                                                                            >  1:598478/1‑238 (MQ=255)
                                                  ggtgGGGATAAACGGCCCCAG‑CACGCCAGCACGCCAGCGAACGGGCCGAGGCCGAC‑GGCGACCACGAACAGCATGGCG‑AAGACCATTT‑CGTTAATGGC‑GCGGCAGGCTTCCATCAGGCGGCGAACGGGCTGGTAACCCCACCACGGCCCCAGGTTTTC‑GGCGCTCATCAGGCCAAAGGGTATGGAGAG‑AA‑CCACCGCCAGCGCGGTGCCCCAGACGGCGATTTGCAGCGTGACGGCCa                                                                                                                                                                                        <  1:1285371/238‑1 (MQ=255)
                                                        gaaaaaagcccccag‑cccgccggccccCCAGGAGACGGGCGGGGGCCGGC‑GGCGCCCCCGAACAGCATGGCG‑AAGACCTTTT‑CTTTAAGGGC‑GCGGCGGGCTTCCATCAGGCGGCGAACGGGCTGGTAAACCCACCACGGCCCCAGGTTTTC‑GGCGCTCATCAGGCCAAAGGGGATGGAGAG‑AA‑CCACCGCCAGCGCGGTGCCCCAGACGGCGATTTGCAGCGTGACGGCCATTTCgg                                                                                                                                                                                  <  2:651953/227‑1 (MQ=255)
                                                        gATAAACAGCGCCAG‑CACGCCAGCACGCCAGCGAACGGGCCGAGGCCGAC‑GGCGACCACGAACAGCATGGCG‑AAGACCATTT‑CGTTAATGGC‑GCGGCAGGCGTCCATCAGGCGGCGAACGGGCTGGTAAACCCACCACGGCACCAGGTTTTC‑GGCGCTCATCAGGCCAACGGGGATGGGGAG‑AA‑CCACCGCCAGCCCGGTGCCCCAGACGGCGGTTTGCAGCGGGACGGCCATTTCGGt                                                                                                                                                                                 >  1:14991/1‑239 (MQ=255)
                                                           aaccAGCGCCAC‑CACGCCAGCACGCCAGTGAAGGGGCCGAGGCCGCC‑GGCGACCACGAACAGCATGGCG‑AAGACCATTT‑CGTTAATGGC‑GCGGCAGGCGTCCATCAGGCGGCGAACGGGCTGGTAAACCCACCACGGCACCAGGTTTTC‑GGCGCTCATCAGGCCAAAGGGGATGGAGAG‑AA‑CCACCGCCAGCGCGGTGCCCCAGACGGCGATTTGCAGCGTGACGGCCATTTCGGTGAg                                                                                                                                                                              <  1:163854/236‑1 (MQ=255)
                                                           aaaCAGCGCCAG‑CACGCCAGCACGCCAGCGAACGGGCCGAGGCCGAC‑GGCGACCACGAACAGCATGGCG‑AGGACCATTT‑CGTTAATGGC‑GCGGCAGGCGTCCATCAGGCGGCGAACGGGCTGGTAAACCCACCCCGGCACCAGGTTTTC‑GGCGCTCATCAGGCCCAAGGGGATGGGGAG‑AA‑ACACCGCCAGCGCGGGGCCCCAGACGGCGATTTTCCACGTGACGGCCCCTTCgggggg                                                                                                                                                                              >  2:534152/1‑235 (MQ=255)
                                                             aCAGCGCCAG‑CACGCCAGCACGCCAGCGAACGGGCCGAGGCCGAC‑GGCGACCACGAACAGCATGGCG‑AAGACCATTT‑CGTTAATGGC‑GCGGCAGGCGTCCATCAGGCGGCGAACGGGCTGGTAAACCCACCACGGCACCAGGTTTTC‑GGCGCTCATCAGGCCAAAGGGGATGGGAAG‑AA‑CCACCGCCCGCCCGGGGCCCCAGCAGGGGCTTTGCAGCGTGGCGGCCATTTCGGTGGGGt                                                                                                                                                                            >  1:277346/1‑239 (MQ=255)
                                                              ccggccccggcccgccggcccgccggcGACGGGGCGGAGGCGGAC‑GGCGACCCCGACCAGAAGGGCG‑AAGCCCATTG‑CGTTAATGGC‑GCGGGGGGCGCCCAGCAGGCGGCGAACGGGCTGGAAAACCCACCACGGCCCCAGGTTTGC‑GGCGCTCATCAGGCCAAAGGGGATGGAGAG‑AA‑CCACCGCCAGCGCGGTGCCCCAGACGGCGATTTGCAGCGTGACGGCCATTTCGGTGAGGt                                                                                                                                                                            <  2:177222/227‑1 (MQ=255)
                                                                gcgcCAG‑CACGCCAGCACGCCAGCGAACGGGCCGAGGCCGAC‑GGCGACCACGAACAGCATGGCG‑AAGACCATTT‑CGTTAATGGC‑GCGGCAGGCGTCCATCAGGCGGCGAACGGGCTGGTAAACCCACCACGGCACCAGGTTTTC‑GGCGCTCATCAGGCCAAAGGGGATGGAGAG‑AA‑CCACCGCCAGCGCGGGGCCCCCGACGGCGATTTGCAGCGGGACGGCCATTTCGGTGAGGTaa                                                                                                                                                                          >  1:1046485/1‑238 (MQ=255)
                                                                  gccag‑cacgccagcacGCCAGCGAACGGGCCGAGGCCGAC‑GGCGACCACGAACAGCATGGCG‑AAGACCATTT‑CGTTAATGGC‑GCGGCAGGCGTCCATCAGGCGGCGAACGGGCTGGTAAACCCACCACGGCACCAGGTTTTC‑GGCGCTCATCAGGCCAAAGGGGATGGAGAG‑AA‑CCACCGCCAGCGCGGTGCCCCAGACGGCGATTTGCACCGTGACGGCCATTTCGGTGAGGTAATc                                                                                                                                                                        >  1:410158/1‑238 (MQ=255)
                                                                   ccag‑cacgccagcacgCCAGCGAACGGGCCGAGGCCGAC‑GGCGACCACGAACAGCATGGCG‑AAGACCATTT‑CGTTAATGGC‑GCGGCAGGCGTCCATCAGGCGGCGAACGGGCTGGTAAACCCACCACGGCCCCAGGTTTTC‑GGCGCTCATCAGGCCAAAGGGGATGGAGAG‑AA‑CCACCGCCAGCGCGGTGCCCCAGACGGCCATTTGCAGCGTGACGGCCAATTCGGTGAGGGAATcc                                                                                                                                                                       >  1:792493/1‑238 (MQ=255)
                                                                         caggccccccccccgcGAAAGGGGCGGGGCCGGCGGGCGCCCCGGAAAAGAATGGGG‑AA‑ACCCTTT‑CGTTTAAGGCGGCGGAGGGCGCCCATAGGGCGGGCAAGGGGTGGGAAACCCCACCACGGCACCAGGTTTTC‑GGCGCTCATGAGGCCAAAGGGGTGGGGGAG‑AA‑CCACCGCCAGCGCGGTGCCCCAGACGGCGATTTGCAGCGTGACGGCCATTTCGGTGAGGTAATCCTGCCa                                                                                                                                                                  <  2:795918/225‑1 (MQ=255)
                                                                                       gcGAACGGGCCGAGGCCGAC‑GGCGCCCACGAACAGAAGGGCG‑AAGACCTTTT‑CGGTAAGGGG‑GCGGCAGGCGTCCATCGGGCGGCGAAGGGGCTGGTAAACCCACCACGGCACCGGGTTTGG‑GGCGCTCATCAGGCAAAGGGGGATGGAGAG‑AA‑CCCCCGCCAGCGCGGTGCCCCAGACGGCGATTTGCAGCGTGACGGCCATTTCGGTGAGGTAATCCTGCCACTGGCTGAAATcggg                                                                                                                                                   <  2:1043036/239‑1 (MQ=255)
                                                                                        gcacggggccggggccgAC‑GGCGCCCACGAAAAGCAGGGCG‑AAGTCCATTG‑CGTTAAGGGC‑GCGGCAGGCGTCCATCAGGCGGCGAACGGGTTGTTAACCCCACCGCGGCACCAGGTTTGC‑GGCGCACATCAGGCCAAGGGGGATGGAGAG‑AA‑CCACCGCCAGCGCGGTGCCCCAGACGGCGATTTGCAGCGTGACGGCCATTTCGGTGAGGTAATCCTGCCACTGGCTGAAATcgggc                                                                                                                                                  <  2:277346/234‑1 (MQ=255)
                                                                                            cGGGCCGAGGCCGAC‑GGCGACCACGAACAGCATGGCG‑AAGACCATTT‑CGTTAATGGC‑GCGGCAGGCGTCCATCAGGCGGCGAACGGGCTGGTAAACCCACCACGGCACCAGGTTTTC‑GGCGCTCATCAGGCCAAAGGGGATGGAGAG‑AA‑CCACCGCCAGCGCGGTGCCCCAGACGGCGATTTGCAGCGTGACGGCCATTTCGGTGAGGTAATCCTGCCACTGGCTGAAATCGGGCGGga                                                                                                                                              >  1:996197/1‑239 (MQ=255)
                                                                                                         aC‑GGCGACCACGAACAGCATGGCG‑AAGACCATTT‑CGTTAATGGC‑GCGGCAGGCGTCCATCAGGCGGCGAACGGGCTGGTAAACCCACCACGGCACCAGGTTTTC‑GGCGCTTATCAGGCCAAAGGGGATGGAGAG‑AA‑CCACCGCCAGCGCGGTGCCCCAGACGGCGGTTTGCAGCGTGACGGCCATTTCGGTGAGGGAATCCTGCCACTGGCTGAAATAGGGCGGGAAGCAGTC‑ggcggc                                                                                                                                >  1:587083/1‑239 (MQ=255)
                                                                                                         aC‑GGCGACCACGAACAGCATGGCG‑AAGACCATTT‑CGTTAATGGC‑GCGGCAGGCGTCCATCAGGCGGCGAACGGGCTGGTAAACCCACCACGGCACCAGGTTTTC‑GGCGCTCATCAGGCCAAAGGGGATGGAGAG‑AA‑CCACCGCCAGCCCGGTGCCCCAGACGGCGATTTGCAGCGTGGCGGCCATTTCGGTGAGGTAATCCTGCCACTGGGTGAAAACGGGGGGGaagaagaggggggg                                                                                                                                 >  2:490005/1‑231 (MQ=255)
                                                                                                         aC‑GGCGACCACGAACAGCATGGCG‑AAGACCATTT‑CGTTAATGGC‑GCGGCAGGCGTCCATCAGGCGGCGAACGGGCTGGTAAACCCACCACGGCACCAGGGTTTC‑GGCGCTCCTCAGGCCAAAGGGGATGGAGAG‑AA‑CCACCGCCAGCGCGGGGCCCCAGACGGCGATTTGCAGCGTGACGGCCCTTTCCGTGGGGTAATCCTCCCCCTGGCGGAAAACGGGGGGggagggacggggggg                                                                                                                                 >  2:918743/1‑228 (MQ=255)
                                                                                                              cgacaccgacaaGCAGGGGG‑AAGACCATTT‑CGTTAAGGGC‑GCGGCAGGCGTCCAGCAGGCGGCGAACGGGCGGGTAAACCCACCACGGCACCAGGTTTGC‑GGCGCTCATCAGGCAAAAGGGGATGGAGAG‑AA‑CCACCGCCAGCGCGGTGCCCCAGACGGCGATTTGCAGCGTGACGGCCATTTCGGTGAGGTAATCCTGCCACTGGCTGAAATCGGGCGGGAAGAAGTC‑GGCGGCGaa                                                                                                                             <  1:7296/227‑1 (MQ=255)
                                                                                                                aCCACGAACAGCATGGCG‑AAGACCATTT‑CGTTAATGGC‑GCGGCAGGCGTCCATCAGGCGGCGAACGGGCTGGTAAACCCACCACGGCACCAGGTTTTC‑GGCGCTCATCAGGCCAAAGGGGATGGAGAG‑AA‑CCACCGCGAGCGCGGTGCCCCAGACGGCGGTTTTCAGCGTGACGGACCTTTCGGGGAGGGAATCCTGCCACCGGGCGAAAGcggggggggcgaaagcggggggagagcc                                                                                                                           >  2:1148785/1‑219 (MQ=255)
                                                                                                                           cATGGCG‑AAGACCATTT‑CGTTAATGGC‑GCGGCAGGCGTCCATCAGGCGGCGAACGGGCTGGTAAACCCACCACGGCACCAGGTTTTC‑GGCGCTCATCAGGCCAAAGGGGATGGAGAG‑AA‑CCACCGCCAGCGCGGTGCCCCAGACGGCGATTTGCAGCGTGACGGCCATTTCGGCGAGGCAATCCTGCCACTGGCTGAAATCGGGCGGGCAGATGTC‑GGCGGCGAACGGCGCCATGTTg                                                                                                                >  1:541395/1‑238 (MQ=255)
                                                                                                                           cATGGCG‑AAGACCATTT‑CGTTAATGGC‑GCGGCAGGCGTCCATCAGGCGGCGAACGGGCTGGTAAACCCACCACGGCACCAGGTTTTC‑GGCGCTCATCAGGCCAAAGGGGATGGAGAG‑AA‑CCACCGCCAGCGCGGTGCCCCAGACGGCGATTTGCAGCGTGAAGGCCATTTCGGTGAGGTAATCCTGCCACTGGCTGAAATCGGGCGGGAAGAAGAC‑GTGGGCGAACGTCCCCTGGTTg                                                                                                                >  1:450380/1‑238 (MQ=255)
                                                                                                                           cATGGCG‑AAGACCATTT‑CGTTAATGGC‑GCGGCAGGCGTCCATCAGGCGGCGAACGGGCTGGTAAACCCACCACGGCACCAGGGTTTC‑GGCGCTCATCAGGCCCAAGGGGATGGAGAG‑AA‑CCACCGCCAGCGCGGTGCCCCAGACGGCGAATTGCAGCGTGACGGCCATTTCGGGGGGGGAATCCTGCCCCCGGCTGAAACCGGGCGGGAAGAAGGC‑GGGGGGGAAAGTCCCCagtatgg                                                                                                               >  2:297017/1‑233 (MQ=255)
                                                                                                                            aTGGCG‑AAGACCATTT‑CGTTAATGGC‑GCGGCAGGCGTCCATCAGGCGGCGAACGGGCTGGTAAACCCACCACGGCACCAGGTTTTC‑GGCGCTCATCAGGCCAAAGGGGATGGAGAG‑AA‑CCACCGCCAGCGCGGTGCCCCAGACGGCGATTTGCAGCGTGACGGCCATTTCGGTGAGGTAATCCTGCCACTGGCTGAAATCGGGCGGGAAGAAGTC‑GGGGGCGAACGTCGCCAGGTTgc                                                                                                               >  1:183150/1‑238 (MQ=255)
                                                                                                                              gggg‑AAGTCCTTTG‑CGTTAAGGGG‑GCGGCAGGCGTCCACGAGGGGGCGAACGGGGTGGTAAACCCCCCCGGGCACCAGGTTGTC‑GGGGCTCATAAGCCAAAGGGGGAGGGGGAG‑AC‑CCCCCGCCAGCGCGGTGCCCCAGCCGGCGATTTGCAGCGTGACGGCCATTTCGGTGAGGTAATCCTGCCACTGGCTGAAATCGGGCGGGAAGAAGTC‑GGCGGCGAACGTCGCCATGTTgccgc                                                                                                            <  2:996197/236‑1 (MQ=255)
                                                                                                                                gggaggccctttt‑CGTTAGGGGG‑GGGGCGGGCCTCCTGCAGGGGGGAAGCGGGGTGGCAAACCCCCCACGGCCCAAGGTTTCG‑GGCCCCAACCAGGCAAAGGGGGAGGGAGAG‑AA‑CCCCCGCCAGCGCGGTGCCCCAGACGGCGATTTGCAGCGTGACGGCCATTTCGGTGAGGTAATCCTGCCACTGGCTGAAATCGGGCGGGAAGAAGTC‑GGCGGCGAACGTCGCCATGTTgccgc                                                                                                            <  2:748504/228‑1 (MQ=255)
                                                                                                                                gcaaaGCCCATTT‑CGTTAAGGGC‑GCGGCAGGGGCCCATCAGGCGGCGACCGGGTTGGTAAACCCACCAGGGCACCAGGTTTTC‑GGCGCTCATCAGGCCAAAGGGGATGGAGGG‑AA‑CCACCGCCAGCGCGGTGCCCCAGACGGCGATTTGCAGCGTGACGGCCATTTCGGTGAGGTAATCCTGCCACTGGCTGAAATCGGGCGGGAAGAAGTC‑GGCGGCGAACGTCGCCATGTTgccgc                                                                                                            <  1:1121709/235‑1 (MQ=255)
                                                                                                                                 gggaagccttttgctttgaTGGG‑GCGGCGGGGGTCGATGAGGGGGGCAAGGGGGGGGAAACCCCCCCCCGGCCCCAGGTTTGC‑GGGGCCAATCAGGCCAAAGGGGAGGGAGAG‑AA‑CCACCGCCAGCGCGGTGCCCCAGACGGCGATTTGCAGCGTGACGGCCATTTCGGTGAGGTAATCCTGCCACTGGCTGAAATCGGGCGGGAAGAAGTC‑GGCGGCGAACGTCGCCATGTTgccgc                                                                                                            <  2:410158/220‑1 (MQ=255)
                                                                                                                                    aGACCATTT‑CGTTAATGGC‑GCGGCAGGCGTCCATCAGGCGGCGAACGGGCTGGTAAACCCACCACGGCACCAGGTTTTC‑GGCGCTCATCAGGCCAAAGGGGATGGAGAG‑AA‑CCACCGCCAGCGCGGTGCCCCAGACGGCGATTTg                                                                                                                                                                                                      >  1:1004283/1‑145 (MQ=255)
                                                                                                                                      aCCATTT‑CGTTAATGGC‑GCGGCAGGCGTCCATCAGGCGGCGAACGGGCTGGTAAACCCACCACGGCACCAGGTTTTC‑GGCGCTCATCAGGCCAACGGGGATGGACAG‑AA‑CCACCGCCAGCGCGGTGCCCCAGACGGCGATTTGACGCGTGACGGCCATTTCGGGGAGGTAATCCTGCCCCCGGCGTAAAACGGGGGGGAAGAAGCG‑GGCGGGGCAGGTCGCCATTTTGCCGCGGCCttg                                                                                                     >  2:1221636/1‑238 (MQ=255)
                                                                                                                                      aCCATTT‑CGTTAATGGC‑GCGGCAGGCGTCCATCAGGCGGCGAACGGGCTGGTAAACCCACCACGGCACCAGGTTTTC‑GGCGCTCATCAGGCCAAAGGGGATGGAGAG‑AA‑CCACCGCCAGCGCGGTGCCCCAGACGGCGATTTGCAGCGTGACGGCCATTTCGGTGAGGTAATCCTGCCCCTGGCTGAAATCGGGCGGGAAGAAGTC‑GGCGGCGAACGTCCCCATGTTGCCGCCGTCttt                                                                                                     >  1:118307/1‑239 (MQ=255)
                                                                                                                                      aCCATTT‑CGTTAATGGC‑GCGGCAGGCGTCCATCAGGCGGCGAACGGGCTGGTAAACCCACCACGGCACCAGGTTTTC‑GGCGCTCATCAGGCCAAAGGGGATGGAGAG‑AA‑CCACCGCCAGCGCGGTGCCCCAGACGGCGATTTGCAGCGTGACGGCCATTTCGGGGGGGGAATCCTGGACCTGGCGGGAAGCGGGGGGGGAAAAGGC‑GGCGGGGCACGTccccccgtcggccgcggcatt                                                                                                     >  2:881602/1‑222 (MQ=255)
                                                                                                                                         aTTT‑CGTTAAGGGC‑CGCGCGGGGTCCCAACGGGGGGCGAAGGGGCGGGAAACCCCCCCCCGGCCCCAGGTTTCC‑GGCCCTCAACAGGCCAAAGGGGATGGAGAG‑AA‑CCACCGCCAGCGCGGTGCCCCAGACGGCGATTTGCAGCGTGACGGCCATTTCGGTGAGGTAATCCTGCCACTGGCTGAAATCGGGCGGGAAGAAGTC‑GGCGGCGAACGTCGCCATGTTGCCGCCGTCTTTGAt                                                                                                  <  1:804234/239‑1 (MQ=255)
                                                                                                                                          ttt‑CGTTAATGGC‑GCGGCAGGCGTCCATCAGGCGGCGAACGGGCTGGTAAACCCACCACGGCACCAGGTTTTC‑GGCGCTCATCAGGCCAAAGGGGATGGAGAG‑AA‑CCACCGCCAGCGCGGTGCCCCAGACGGCGATTTGCAGCGTGACGGCCATTTCGGTGAGGTAATCCTGCCACTGGCTGAAATCGGGCGGGAAGAAGTC‑GGCGGCGAACGTCGCCATGTTGCCGCCGCCTTTGAt                                                                                                  >  1:216478/1‑238 (MQ=255)
                                                                                                                                          ttt‑CGTTAATGGC‑GCGGCAGGCGTCCATCAGGCGGCGAACGGGCTGGTAAACCCACCACGGCACCAGGTTTTC‑GGCGCTCATCAGGCCAAAGGGGATGGAGAG‑AA‑CCACCGCCAGCGCGGTGCCCCAGACGGCGATTTGCAGCGGGACGGCCATTTCGGGGAGGTAATCCTGCCACTGGCTGAAATCGGGCGGGAAGAAGTC‑GGGGGCGAACTTCGCCATGTTGCCGCCGTCTTTGGTc                                                                                                 >  1:985774/1‑239 (MQ=255)
                                                                                                                                          ttgtgtttAAGGGC‑GGGGCGGGCGCCCAGCGGGC‑GCGAAAGGGGCGGGTAACCCCCCGCGGCGCCCGGGTTTCGGGCGCTCACCAGGCAAAGGGGGATGGAGAG‑AC‑CCCCCGCCAGCGCGGTGCCCCAGACGGCGATTTGCAGCGTGACGGCCATTTCGGGGAGGTAATCCTGCCACTGGCTGAAATCGGGCGGGAAGAAGTC‑GGCGGCGAACGTCGCCATGTTGCCGCCGTCTTTGAt                                                                                                  <  2:1148936/233‑1 (MQ=255)
                                                                                                                                                ttAATGGC‑GCGGCAGGCGTCCATCAGGCGGCGAACGGGCTGGTAAACCCACCACGGCACCAGGTTTTC‑GGCGCTCATCAGGCCAAAGGGGATGGAGAG‑AA‑CCACCGCCAGCGCGGTGCCCCAGACGGCGATTTGCAGCGTGACGGCCCTTTCGGTGAGGTAATCCTGCCACTGGCTGAAATCGGGCGGGAAGCAGTC‑GGCGGCGAACGTCGCCATGTTGCCGCCGCCTTTGATCAGCgg                                                                                            >  1:1247379/1‑238 (MQ=255)
                                                                                                                                                   aTGGC‑GCGGCAGGCGTCCATCAGGCGGCGAACGGGCTGGTAAACCCACCACGGCACCAGGGTTTC‑GGCGCTCATCAGGCCAAAGGGGATGGAGAG‑AA‑CCACCGCCAGCGCGGTGCCCCAGACGGCGATTTGCAGCGTGACGGCCATTTCCGGGGGGGAAACCTCCCCCTGGCTGAAAAAGGGCGGGAAGAAGTCGGGGGGCGACCG‑CGCCGTGTTGCCGCCCGATTTGACCAGCgggag                                                                                         >  2:851901/1‑235 (MQ=255)
                                                                                                                                                                   tCCATCAGGCGGCGAACGGGCTGGTAAACCCACCACGGCACCAGGTTTTC‑GGCGCTCATCAGGCCAAAGGGGATGGAGAG‑AA‑CCACCGCCAGCGCGGTGCCCCAGACGGCGATTTGCAGCGTGACGGCCATTTCGGTGAGGTAATCCTGCCACTGGCTGAAATCGGGCGGGAAGAAGTC‑GGCGGCGACCGTCGCCATGTTGCCGCCGCCTTTGATCAGCGTAAGCGGGGCCATTTCcg                                                                           >  1:342846/1‑238 (MQ=255)
                                                                                                                                                                   tCCATCAGGCGGCGAACGGGCTGGTAAACCCACCACGGCACCAGGTTTTC‑GGCGCTCATCAGGCCAAAGGGGATGGAGAG‑AA‑CCACCGCCAGCGCGGTGCCCCAGACGGCGATTTGCAGCGTGACGGCCATTTCGGTGAGGTAATCCTGCCACTGGCTGAAATCGGGCGGGAAGAAGTC‑GGCGGCGAACGTCGCCATGTTGCCGCCGTCCTTGATCAGCGGTACCGGGGCCCTTTccc                                                                           >  1:105440/1‑237 (MQ=255)
                                                                                                                                                                      cctggggggacacggggcgagTAAACCC‑CCACGGCGCCGGGTTTTT‑CGCCGTAATCAAGGCAAAGGGGCGAGGGAGAAA‑CCACCGCAGGCGCGGTGACCCAGAGGGCGTGTTGCAGCGTGACGGCCATTTGGGTGAGTTAATCCTGCCACTGGCTGAAATCGGGCGGGAAGAAGTC‑GGCGGCGAACGTCGCCATGTTGCCGCCGTCTTTGATCAGCGTAAGCGGGGCCATTTCCGCGcc                                                                       <  2:985774/219‑1 (MQ=255)
                                                                                                                                                                                 aaCGGGCTGGAAACCCCCCCGCGGCACCGGTTTTTG‑GGCGCTCACAAGGCCAAGGGGGATGGAGAG‑AA‑CCCCCGCCAGCGCGGTGCCCCAGACGGCGATTTGCAGCGTGACGGCCATTTCGGTGAGGTAATCCTGCCACTGGCTGAAATCGGGCGGGAAGAAGTC‑GGCGGCGAACGTCGCCATGTTGCCGCCGTCTTTGATCAGCGTAAGCGGGGCCATTTCCGCGCCCTGCCACgag                                                             <  1:268002/238‑1 (MQ=255)
                                                                                                                                                                                    gggCTGGAAAACCCACCACGGCACCGGTTTTTC‑GGCGCTCACAAGGCCAAAGGGGAGGGAGAG‑AC‑CCCCCGCCAGCGCGGTGCCCCAGACGGCGATTTGCAGCGTGACGGCCATTTCGGTGAGGTAATCCTGCCACTGGCTGAAATCGGGCGGGAAGAAGTC‑GGCGGCGAACGTCGCCATGTTGCCGCCGTCTTTGATCAGCGTAAGCGGGGCCATTTCCGCGCCCTGCCACGAgac                                                           <  1:947056/237‑1 (MQ=255)
                                                                                                                                                                                                ccaccaCGGCACCAGGTTTTC‑GGCGCTCATCAGGCCAAAGGGGATGGAGAG‑AA‑CCACCGCCAGCGCGGTGCCCCAGACGGCGATTTGCAGCGTGACGGCCATTTCGGTGAGGTAATCCTGCCACTGGCTGAAATCGGGCGGGAAGAAGGC‑GGCGGCGCACGTCGCCATGTTTCCGCCGCCTTTGATCAGCGTAAGCGGGGCAATTTCCCGGCACGCCCCCGCGACGACCAACCAGGCgg                                             >  2:333404/1‑238 (MQ=255)
                                                                                                                                                                                                  accacGGCACCAGGTTTTC‑GGCGCTCATCAGGCCAAAGGGGATGGAGAG‑AA‑CCACCGCCAGCGCGGTGCCCCAGACGGCGATTTGCAGCGTGACgg                                                                                                                                                                                           <  2:358609/96‑1 (MQ=255)
                                                                                                                                                                                                  accacGGCACCAGGTTTTC‑GGCGCTCATCAGGCCAAAGGGGATGGAGAG‑AA‑CCACCGCCAGCGCGGTGCCCCAGACGGCGATTTGCAGCGTGACgg                                                                                                                                                                                           >  1:358609/1‑96 (MQ=255)
                                                                                                                                                                                                  accacGGCACCAGGTTTTC‑GGCGCTCATCAGGCCAAAGGGGATGGAGAG‑AA‑CCACCGCCAGCGCGGTGCCCCAGACGGCGATTTGCAGCGTGACGGCCATTTCGGTGAGGTAATCCTGCCACTGGCTGAAATCGGGCGGGAAGAAGTC‑GGCGGCGAACGTCGCCATGTTGCCGCCGTCTTTGATCAGCGTAAGCGGGGCCATTTCCGCGCCCTGCCACGAGACGACCAACACGGCGAgg                                           >  1:1090747/1‑238 (MQ=255)
                                                                                                                                                                                                    cacGGCACCAGGTTTTC‑GGCGCTCATCAGGCCAAAGGGGATGGAGAG‑AA‑CCACCGCCAGCGCGGTGCCCCAGACGGCGATTTGCAGCGTGACGGCCATTTCGGTGAGGTAATCCTGCCACTGGCTGAAATCGGGCGGGAAGAAGTC‑GGCGGCGAACGTCGCCATGTTGCCGCCGTCTTTGATCAGCGTAAGCGGGGCCATTTCCGCGCCCTGCCACGAGACGCCCAACACGGCGGGTAc                                         >  1:984712/1‑239 (MQ=255)
                                                                                                                                                                                                    cacGGCACCAGGTTTTC‑GGCGCTCATCAGGCCAAAGGGGATGGAGAG‑AA‑CCACCGCCAGCGCGGTGCCCCAGACGGCGATTTGCAGCGTGACGGCCATTTCGGTGAGGTAATCCTGCCACTGGCTGAAATCGGGCGGGAAGAAGTC‑GGCGGCGAACGTCGCCATGTTGCCGCCGTCTTTGATCAGCGTAAGCGGGGCCATTTCCGCGCCCTGCCACGAGACGCCCAACACGGCGGGTAc                                         >  1:995039/1‑239 (MQ=255)
                                                                                                                                                                                                    cacGGCACCAGGTTTTC‑GGCGCTCATCAGGCCAAAGGGGATGGAGAG‑AA‑CCACCGCCAGCGCGGTGCCCCAGACGGCGATTTGCAGCGTGACGGCCATTTCGGTGAGGTAATCCTGCCACTGGCTGAAATCGGGCGGGAAGAAGTC‑GGCGGCGAACGTCGCCATGTTGCCGCCGTCTTTGATCAGCGTAAGCGGGGCCATTTCCGCGCCCTGCCACGAGACGCCCAACACGGCGAGTAc                                         >  1:1132132/1‑239 (MQ=255)
                                                                                                                                                                                                      gggCACCAGGTTTTG‑GGCGCCAACGAGGCAAAGGGGGGTGGAGAG‑AA‑CCCCCGCCAGCGCGGTGCCCCAGACGGCGATTTGCAGCGTGACGGCCATTTCGGTGAGGTAATCCTGCCACTGGCTGAAATCGGGCGGGAAGAAGTC‑GGCGGCGAACGTCGCCATGTTGCCGCCGTCTTTGATCAGCGTAAGCGGGGCCATTTCCGCGCCCTGCCACGAGACGACCAACACGGCGAGTACaa                                       <  1:160245/238‑1 (MQ=255)
                                                                                                                                                                                                         cACCAGGTTTTC‑GGCGCTCATCAGGCCAAAGGGGATGGAGAG‑AA‑CCACCGCCAGCGCGGTGCCCCAGACGGCGATTTGCAGCGTGACGGCCATTTCGGTGAGGTAATCCTGCCACTGGCTGAAATCGGGCGGGAAGAAGTC‑GGCGGCGAACGTCGCCATGTTGCCGCCGTCTTTGATCAGCGTAAGCGGGGCCATTTCCGCGCCCTGCCACGAGACGACCAACACGGCGAGTACAACg                                     >  1:197065/1‑238 (MQ=255)
                                                                                                                                                                                                         cACCAGGTTTTC‑GGCGCTCATCAGGCCAAAGGGGATGGAGAG‑AA‑CCACCGCCAGCGCGGTGCCCCAGACGGCGATTTGCAGCGTGACGGCCATTTCGGTGAGGTAATCCTGCCACTGGCTGAAATCGGGCGGGAAGAAGTC‑GGCGGCGAACGTCGCCATGTTGCCGCCGTCTTTGATCAGCGTAAGCGGGGCCATTTCCCCGCCCTGCCACGGGACGACCAACAAGGCGAGGACAACg                                     >  1:300677/1‑238 (MQ=255)
                                                                                                                                                                                                          cccgggttgcc‑ggcggTAATCAGGACAAAGGGGATGGAGAG‑AA‑CCCCCGCCAGCGCGGTGCCCCAGACGGCGATTTGCAGCGTGACGGCCATTTCGGTGAGGTAATCCTGCCACTGGCTGAAATCGGGCGGGAAGAAGTC‑GGCGGCGAACGTCGCCATGTTGCCGCCGTCTTTGATCAGCGTAAGCGGGGCCATTTCCGCGCCCTGCCACGAGACGACCAACACGGCGAGTACAACgg                                    <  1:1148785/228‑1 (MQ=255)
                                                                                                                                                                                                          aCCAGGTTTTC‑GGCGCTCATCAGGCCAAAGGGGATGGAGAG‑AA‑CCACCGCCAGCGCGGTGCCCCAGACGGCGATTTGCAGCGTGACGGCCATTTCGGTGAGGTAATCCTGCCACTGGCTGAAATCGGGCGGGAAGAAGTC‑GGCGGCGAACGTCGCCATGTTGCCGCCGTCTTTGATCAGCGTAAGCGGGGCCATTTCCGCGCCCTGCACCGAGACGACTGACACGGCGAgaccaaagg                                    >  1:1166763/1‑230 (MQ=255)
                                                                                                                                                                                                           ccAGTTTGTG‑GGCGCTCACCGGGCCAAAGGGGATGGAGAG‑AC‑CCACCGCCCGCGGGGGGCCCCAGACGGCGATTTGCAGCGTGACGGCCATTTCGGTGAGGTAATCCTGCCACTGGCTGAAATCGGGCGGGAAGAAGTC‑GGCGGCGAACGTCGCCATGTTGCCGCCGTCTTTGATCAGCGTAAGCGGGGCCATTTCCGCGCCCTGCCACGAGACGACCAACACGGCGAGTACAACGGcc                                  <  1:922539/239‑1 (MQ=255)
                                                                                                                                                                                                           ccAGGTTTGC‑GGCGCCGCACGGCCCAAGGGGTATGGAGGG‑AA‑CCACCGCCAGCGCGGTGCCCCAGACGGCGATTTGCAGCGTGACGGCCATTTCGGTGAGGTAATCCTGCCACTGGCTGAAATCGGGCGGGAAGAAGTC‑GGCGGCGAACGTCGCCATGTTGCCGCCGTCTTTGATCAGCGTAAGCGGGGCCATTTCCGCGCCCTGCCACGAGACGACCAACACGGCGAGTACAACGGcc                                  <  1:851901/239‑1 (MQ=255)
                                                                                                                                                                                                                 tttC‑GGCGCTCATCAGGCCAAAGGGGATGGAGAG‑AA‑CCACCGCCAGCGCGGTGCCCCAGACGGCGATTTGCAGCGTGACGGCCATTTCGGTGAGGTAATCCTGCCACTGGCTGAAATCGGGCGGGAAGAAGTC‑GGCGGCGAACGTCGCCATGTTGCCGCCGTCTTTg                                                                                                    >  1:730242/1‑167 (MQ=255)
                                                                                                                                                                                                                  ttC‑GGCGCTCAACAGGCCAAAGGGGATGGAGAG‑AC‑CCCCCGCCAGCGCGGTGCCCCAGACGGCGATTTGCAGCGTGACGGCCATTGCGGTGAGGTAATCCTGCCACTGGCTGAAATCGGGCGGGAAGAAGTC‑GGCGGCGAACGTCGCCATGTTGCCGCCGTCTTTGATCAGCGTAAGCGGGGCCATTTCCGCGCCCTGCCACGAGACGACCAACACGGCGAGTACAACGGCCCAGCTCa                           <  1:1221636/239‑1 (MQ=255)
                                                                                                                                                                                                                   tccgggccTCATCAGGCAAAGGGGGGTGGAGAG‑AA‑CCCCCCCCAGCGCGGTGCCCCAGCCGGCGATTTGGAGGGGGGCGGCCATTTCGGTGAGGTAATCCTGCCACTGGCTGAAATCGGGCGGGAAGAAGTC‑GGCGGCGAACGTCGCCATGTTGCCGCCGTCTTTGATCAGCGTAAGCGGGGCCATTTCCGCGCCCTGCCACGAGACGACCAACACGGCGAGTACAACGGCCCAGCTCa                           <  1:534152/232‑1 (MQ=255)
                                                                                                                                                                                                                    c‑GGCGCTCATCAGGCCAAAGGGGATGGAGAG‑AA‑CCACCGCCAGCGCGGTGCCCCAGACGGCGATTTGCAGCGTGACGGCCATTTCGGTGAGGTAATCCTGCCACTGGCTGAAATCGGGCGGGAAGAAGTC‑GGCGGCGAACGTCGCCCTGTTGCCGCCGTCTTTGATCAGCGTAAGCGGGGCCATTTCCGCGCCCTGCCCCCAGACGACCAACACGGCGGGGACAACCGccccccccaca                         >  2:768971/1‑231 (MQ=255)
                                                                                                                                                                                                                      gccgggaattgtgaaaaaGGGAAGGGGGAG‑AACCCACCGCCAGCGCGGTGCCCCAGACGGCGATTTGCAGCGTGACGGCCATTTCGGTGAGGTAATCCTGCCACTGGCTGAAATCGGGCGGGAAGAAGTC‑GGCGGCGAACGTCGCCATGTTGCCGCCGTCTTTGATCAGCGTAAGCGGGGCCATTTCCGCGCCCTGCCACGAGACGACCAACACGGCGAGTACAACGGCCCAGCTCAg                          <  1:114379/223‑1 (MQ=255)
                                                                                                                                                                                                                         gcTCATCAGGCCAAAGGGGATGGAGAG‑AA‑CCACCGCCAGCGCGGTGCCCCAGACGGCGATTTGCAGCGTGACGGCCATTTCGGTGAGGTAATCCTGCCACTGGCTGAAATCGGGCGGGAAGAAGTC‑GGCGGCGAACGTCGCCATGTTGCCGCCGTCTTTGATCAGCGTAAGCGGGGCCATTTCCGCGCCCTGCCCCGAGACGACCAACACGGCGCGAACAACGGcccccccccaaagag                     >  2:1056718/1‑227 (MQ=255)
                                                                                                                                                                                                                          cTCATCAGGCCAAAGGGGATGGAGAG‑AA‑CCACCGCCAGCGCGGTGCCCCAGACGGCGATTTGCAGCGTGACGGCCATTTCGGTGAGGTAATCCTGCCACTGGCTGAAATCGGGCGGGCAGAAGTC‑GGCGGCGAACGTCGCCATGTTGCCGCCGTCTTTGATCAGCGTAAGCGGGGCCATTTCCGCGCCCTGCCACGAGACGACCAACACGGCGAGGACAACGGCCACGCTCCGAAACga                    >  1:1188275/1‑239 (MQ=255)
                                                                                                                                                                                                                           tcatcaGGCCAAAGGGGATGGAGAG‑AA‑CCACCGCCAGCGCGGTGCCCCAGACGGCGATTTGCAGCGTGACGGCCATTTCGGTGAGGTAATCCTGCCACTGGCTGAAATCGGGCGGGAAGAAGTC‑GGCGGCGAACGTCGCCATGTTGCCGCCGTCTTTGATCAGCGTAAGCGGGGCCATTTCCGCGCCCTGCCACGAGACGACCAACACGGCGAGTACAACGGCCCAGCCCAGAAGCgag                   >  1:532017/1‑239 (MQ=255)
                                                                                                                                                                                                                           cctaccgggcAAAGGGGAGTGGGAG‑AACCCACCGCCAGCGCGGTGCCCCAGCCGGGGATTTGCAGCGTGACGGCCATTTCGGTGAGGTAATCCTGCCACTGGCTGAAATCGGGCGGGAAGAAGTC‑GGCGGCGAACGTCGCCATGTTGCCGCCGTCTTTGATCAGCGTAAGCGGGGCCATTTCCGCGCCCTGCCACGAGACGACCAACACGGCGAGTACAACGGCCCAGCTCAGAAGCg                     <  1:1231354/229‑1 (MQ=255)
                                                                                                                                                                                                                                  gCCAAAGGGGATGGAGAG‑AA‑CCACCGCCAGCGCGGTGCCCCAGACGGCGATTTGCAGCGTGACGGCCATTTCGGTGAGGTAATCCTGCCACTGGCTGAAATCGGGCGGGAAGAAGTC‑GGCGGCGAACGTCGCCATGTTGCCGCCGTCTTTGATCAGCGTAAGCGGGGCCATTTCCGCGCCCTGCCACGAGACGACCAACACGGCGAGTCCAACGGCCACGCTCAGACGCGAGAACCa              >  1:411077/1‑237 (MQ=255)
                                                                                                                                                                                                                                   caaagggggATGAGGAG‑AC‑CCACCGCCGGGGGGGTGCCCCAGACGGCGATTTGCCGCGTGACGGCCATTTCGGTGAGGTAATCCTGCCACTGGCTGAAATCGGGCGGGAAGAAGTC‑GGCGGCGAACGTCGCCATGTTGCCGCCGTCTTTGATCAGCGTAAGCGGGGCCATTTCCGCGCCCTGCCACGAGACGACCAACACGGCGAGTACAACGGCCCAGCTCAGAAGCGAGAACCAGc            <  1:1305151/233‑1 (MQ=255)
                                                                                                                                                                                                                                       aGGGGATGGAGAG‑AA‑CCACCGCCAGCGCGGTGCCCCAGACGGCGATTTGCAGCGTGACGGCCATTTCGGTGAGGTAATCCTGCCACTGGCTGAAATCGGGCGGGAAGAAGTC‑GGCGGCGAACGTCGCCATGTTGCCGCCGTCTTTGATCAGCGTAAGCGGGGCCATTTCCGCGCCCTGCCACGAGACGACCAACACGGCGAGTCCAACGGCCCACCTCAGAAGCGAGAACCAGCCgc         >  1:759483/1‑237 (MQ=255)
                                                                                                                                                                                                                                       aGGGGATGGAGAG‑AA‑CCACCGCCAGCGCGGTGCCCCAGACGGCGATTTGCAGCGTGACGGCCATTTCGGTGAGGTAATCCTGCCACTGGCTGAAATCGGGCGGGAAGAAGTC‑GGCGGCGAACGTCGCCATGTTGCCGCCGTCTTTGATCAGCGTAAGCGGGGCCATTTCCGCGCCCTGCCACGAGACGACCAACACGGCGAGTACAACGGCCCAGCTCCGAAGCGAGAACCAGCTgc         >  1:215584/1‑237 (MQ=255)
                                                                                                                                                                                                                                            aTGGAGAG‑AA‑CCACCGCCAGCGCGGTGCCCCAGACGGCGATTTGCAGCGTGACGGCCATTTCGGTGAGGTAATCCTGCCACTGGCTGAAATCGGGCGGGAAGAAGTC‑GGCGGCGAACGTCGCCATGTTGCCGCCGTCTTTGATCAGCGTAAGCGGGGCCATTTCCGCGCCCTGCCCCCGGCCGGCCAACCCGGCGAGGACAACCGGCCACGCCCGACGCGAGAAcaacccgccgtgggg  >  2:1208543/1‑225 (MQ=255)
                                                                                                                                                                                                                                            aTGGAGAG‑AA‑CCACCGCCAGCGCGGTGCCCCAGACGGCGATTTGCAGCGTGACGGCCATTTCGGTGAGGTAATCCTGCCACTGGCTGAAATCGGGCGGGAAGAAGTC‑GGCGGCGAACGTCGCCATGTTGCCGCCGTCTTTGATCAGCGTAAGCGGGGCCATTTCCGCGCCCTGCCACGAGACGACCAACACGGCGAGTACAACGGCCCAGCTCAGAAGCGAGAACCAGCTGCGCTtggg  >  1:590818/1‑239 (MQ=255)
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                             
GGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAACAGCGCCAG‑CACGCCAGCACGCCAGCGAACGGGCCGAGGCCGAC‑GGCGACCACGAACAGCATGGCG‑AAGACCATTT‑CGTTAATGGC‑GCGGCAGGCGTCCATCAGGCGGCGAACGGGCTGGTAAACCCACCACGGCACCAGGTTTTC‑GGCGCTCATCAGGCCAAAGGGGATGGAGAG‑AA‑CCACCGCCAGCGCGGTGCCCCAGACGGCGATTTGCAGCGTGACGGCCATTTCGGTGAGGTAATCCTGCCACTGGCTGAAATCGGGCGGGAAGAAGTC‑GGCGGCGAACGTCGCCATGTTGCCGCCGTCTTTGATCAGCGTAAGCGGGGCCATTTCCGCGCCCTGCCACGAGACGACCAACACGGCGAGTACAACGGCCCAGCTCAGAAGCGAGAACCAGCTGCGCTTGGG  >  NC_000913/4322739‑4323207

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A