Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F27 I0 R1 120 139.6 2835800 98.5% 2793263 226.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,004,469 G→C 12.0% C76W (TGC→TGG)  yigI ← 4HBT thioesterase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,004,4690GC12.0% 258.4 / 7.1 100C76W (TGC→TGG) yigI4HBT thioesterase family protein
Reads supporting (aligned to +/- strand):  ref base G (9/78);  new base C (11/2);  total (20/80)
Fisher's exact test for biased strand distribution p-value = 7.61e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 104 total aligned reads displayed.

AACCTACCATATAGGTGGCGGTGGCACTGGCAATATAAAGCTGTTCTTCATTGTGTAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGC‑GGGTTAA‑GGTAC‑‑TTCCCA‑CGCAC‑ACCAGACCGGCGGCGACATCCAGCGCCGACG‑CAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCG‑AACTCTTTTTCGTAACGCTCCAGTTCCATCCCCA‑ATGCGCGGTTAAATGGCATGTGATAAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACGGCAGACATATCATCCTTA  >  NC_000913/4004231‑4004706
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                  
aaCCTACCATATAGGTGGCGGTGGCACTGGCAATATAAAGCTGTTCTTCATTGTGTAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGC‑GGGTTAA‑GGTAC‑‑TTCCCA‑Cg                                                                                                                                                                                                                                                    <  2:1371717/239‑1 (MQ=255)
aaCCTACCATATAGGTGGCGGTGGCACTGGCAATATAAAGCTGTTCTTCATTGTGTAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGC‑GGGTTAA‑GGTAC‑‑TTCCCA‑Cg                                                                                                                                                                                                                                                    <  2:253109/239‑1 (MQ=255)
aaCCTACCATATAGGTGGCGGTGGCACTGGCAATATAAAGCTGTTCTTCATTGTGTAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGC‑GGGTTAA‑GGTAC‑‑TTCCCA‑Cg                                                                                                                                                                                                                                                    <  2:940435/239‑1 (MQ=255)
               tggcggtggcACTGGCAATATAAAGCTGTTCTTCATTGTGTAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGC‑GGGTTAA‑GGTAC‑‑TTCCCA‑CGCAC‑ACCAGACcgg                                                                                                                                                                                                                                      <  1:391018/237‑1 (MQ=255)
                ggcggtggcACTGGCAATATAAAGCTGTTCTTCATTGTGTAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGC‑GGGTTAA‑GTGAC‑‑TTTCCA‑CCCCC‑Accccaacggggc                                                                                                                                                                                                                                   >  1:840279/1‑229 (MQ=255)
                ggcggtggcACTGGCAATATAAAGCTGTTCTTCATTGTGTAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGC‑GGGTTAA‑AGGAC‑‑TTCCCC‑CCCAC‑ACCCCAAcggcg                                                                                                                                                                                                                                    >  1:1382184/1‑238 (MQ=255)
                ggcggtggcACTGGCAATATAAAGCTGTTCTTCATTGTGTAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGC‑GGGGTTA‑GGGAA‑‑GTCCCC‑CCCcc‑accacccccgccc                                                                                                                                                                                                                                   >  2:838776/1‑230 (MQ=255)
                ggcggtggcACTGGCAATATAAAGCTGTTCTTCATTGTGTAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGC‑GGGGTAT‑GGTGC‑‑TTCCCC‑CCCCC‑ACCacacacgcc                                                                                                                                                                                                                                    >  1:571040/1‑233 (MQ=255)
                 gcggtggcACTGGCAATATAAAGCTGTTCTTCATTGTGTAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGGGTTAG‑GGTAT‑‑TTCCTC‑CCCAC‑CCCAGcccccgg                                                                                                                                                                                                                                    >  2:658351/1‑234 (MQ=255)
                 gcggtggcACTGGCAATATAAAGCTGTTCTTCATTGTGTAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGC‑GGGTGAT‑GGTACGTTTCCCA‑CCCCC‑CCCACCCcggcg                                                                                                                                                                                                                                    >  2:1038929/1‑239 (MQ=255)
                 gcggtggcACTGGCAATATAAAGCTGTTCTTCATTGTGTAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGC‑GGGGTTA‑GGTGA‑‑TTCTCC‑CCcac‑accacacacgccgc                                                                                                                                                                                                                                  >  2:615593/1‑232 (MQ=255)
                               aaTATAAAGCTGTTCTTCATTGTGTAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGC‑GGGTTAA‑GGTAC‑‑TTCCCA‑CGCAC‑ACCAGACCGGCGGCGACATCCAGCGcc                                                                                                                                                                                                                     <  1:1038929/238‑1 (MQ=255)
                                 tataAAGCTGTTCTTCATTGTGTAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGC‑GGGTTAA‑GGTAC‑‑TTCCCA‑Cg                                                                                                                                                                                                                                                    <  1:89565/206‑1 (MQ=255)
                                   aaaaGCTGTTCTTCATTGTGTAATCAAGCGGGGGCTAGGGCGACTGTATGGCCTGCACGAAACAGGATACTAGTAGAAGTAAAGCGCTCGCCCCTGCCTGGGCGTAGATAATCAACGCGAAGATCAATTGTCCCCTTCCGCGATGGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGC‑GGGTTAA‑GGTAC‑‑TTCCCA‑CGCAC‑CCCAGACCGGCGGCGACATCCGGCGCCGACG‑c                                                                                                                                                                                                               <  2:658103/238‑1 (MQ=255)
                                       gCTGTTCTTCATTGTGTAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAACCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGC‑GGGTTAA‑GGTGA‑‑TTGCCA‑CCCTCAACCCAACCGGC‑GCGGCATCCatccgcgcgcccaca                                                                                                                                                                                                            >  1:732617/1‑225 (MQ=255)
                                           ttcttcATTGTGTAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGC‑GGGGTAA‑GGTGC‑‑ATCTCACCCCCC‑ACAACACC‑GCGGCGCCCACCCGCGGCCACG‑CAATGaac                                                                                                                                                                                                        >  1:874184/1‑236 (MQ=255)
                                                     tgtAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGC‑GGGTTTA‑GGGGC‑‑TTCTCA‑CGCCC‑ACCACACCCGCGGGGACCTCCCGCGCCGCCG‑Caccgacacccccccgccc                                                                                                                                                                                             >  1:1198741/1‑222 (MQ=255)
                                                                  gggCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGCAGATAATCACCGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGC‑GGGTTAA‑GGTAC‑‑TTCCCA‑CGCAC‑ACCAGACCGGCGGCGACATCCAGCGCCGACG‑CAATGACCCCGCCGTGCAAAATGCTTTGCGcc                                                                                                                                                                                <  1:1011162/239‑1 (MQ=255)
                                                                  gggCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGC‑GGGTTAA‑GGTAC‑‑TTCCCA‑CGCCC‑ACCAGACCGGCGGCGACATCCAGCGCCGACG‑CAATGACCCCGCCGTGCAAAATGCTTTGCGcc                                                                                                                                                                                <  1:487269/239‑1 (MQ=255)
                                                                  gggCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGC‑GGGTTAA‑GGTAC‑‑TTCCCA‑CGCAC‑ACCAGACCGGCGGCGACATCCAGCGCCGACG‑CAATGACCCCGCCGTGCAAAATGCTTTGCGcc                                                                                                                                                                                <  1:458225/239‑1 (MQ=255)
                                                                            gacTTTATTGCCTGCCCGCAACAGGCTACTAGAAGCAGTAAACCGCTCGCCCCTGCCGGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGC‑GGGTTAA‑GGTAC‑‑TTCCCA‑CGCAC‑ACCAGACCGGCGGCGACATCCAGCGCCGACG‑CAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCcca                                                                                                                                                                     <  2:571040/239‑1 (MQ=255)
                                                                            gacTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCACCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGC‑GGGTTAA‑GGTAC‑‑TTCCCA‑CGCAC‑ACCAGACCGGCGGCGACATCCAGCGCCGACG‑CAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCcca                                                                                                                                                                     <  2:29060/239‑1 (MQ=255)
                                                                            gacTTTAGTGCCCCAACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGC‑GGGTTAA‑GGTAC‑‑TTCCCA‑CGCAC‑ACCAGACCGGCGGCGACATCCAGCGCCGACG‑CAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCcca                                                                                                                                                                     <  1:620936/239‑1 (MQ=255)
                                                                               tttATTCCCCGCACGCACACGGCTACTGGTAGCAGTAAAACGCCCGCCCCTGCCGGGGCGCAAATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGC‑GGGTTAA‑GGTAC‑‑TTCCCA‑CGCAC‑ACCAGACCGGCGGCGACATCCAGCGCCGACG‑CAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAggcaggggcga                                                                                                                                                                   <  2:505477/239‑11 (MQ=255)
                                                                                                aCAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGC‑GGGTTAA‑GGTAC‑‑TTCCCA‑CGCAC‑ACCAGACCGGCGGCGACATCCAGCGCCGACG‑CAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTa                                                                                                                                                 <  1:1408260/239‑1 (MQ=255)
                                                                                                     ctactaGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGC‑GGGTTAA‑GGTAC‑‑TTCCCA‑CGCAC‑ACCAGACCGGCGGCGACATCCAGCGCCGACG‑CAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTAAAgg                                                                                                                                             <  2:1320721/238‑1 (MQ=255)
                                                                                                      tactaGTAGCAGTAAAACGCTCGCCCCTGCCGGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGC‑GGGTTAA‑GGTAC‑‑TTCCCA‑CGCAC‑ACCAGACCGGCGGCGACATCCAGCGCCGACG‑CAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGc                                                                                                                                            <  2:837678/238‑1 (MQ=255)
                                                                                                         tagtagCAGTAAAACGCTCGCCCCTGCCTGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGC‑GGGTTAA‑GGTAC‑‑TTCCCA‑CGCAC‑ACCAGACCGGCGGCGACATCCAGCGCCGACG‑CAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGc                                                                                                                                        <  1:942144/239‑1 (MQ=255)
                                                                                                         tagtagCAGTAAAACGCTCGCCCCTGCCTGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGC‑GGGTTAA‑GGTAC‑‑TTCCCA‑CGCAC‑ACCAGACCGGCGGCGACATCCAGCGCCGACG‑CAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGc                                                                                                                                        <  1:419386/239‑1 (MQ=255)
                                                                                                         tagtagCAGTAAAACGCTCGCCCCTGCCTGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGC‑GGGGTAAGGGGAC‑‑TTCCCC‑CCCCC‑ACCA‑CCCCGCGGCGCCACCCACCGCCCCCGCCAACGACCCCCCCCGCCACAACCTTTTGCGCCCCC‑TGCCCCCCCCCCATTTGGTGATTTTTTAAGGccc                                                                                                                                          >  2:1060092/1‑237 (MQ=255)
                                                                                                                aGTAAAACGCTCGCCCCTGCCTGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGC‑GGGTTAA‑GGTAC‑‑TTCCCA‑CGCAC‑ACCAGACCGGCGGCGACATCCAGCGCCGACG‑CAATGCCCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCg                                                                                                                                  <  1:1060092/238‑1 (MQ=255)
                                                                                                                   aaacCGCTCGCCCCTGCCTGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGC‑GGGTTAA‑GGTAC‑‑TTCCCA‑CGCAC‑ACCAGACCGGCGGCGACATCCAGCGCCGACG‑CAACGACCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCG‑aa                                                                                                                               <  1:1283374/237‑1 (MQ=255)
                                                                                                                   aaaaCGCTCGCCCCTGCCTGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGC‑GGGTTAA‑GGTAC‑‑TTCCCA‑CGCAC‑ACCAGACCGGCGGCGACATCCAGCGCCGACG‑CAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCG‑aa                                                                                                                               <  1:1227516/237‑1 (MQ=255)
                                                                                                                             ccccTGCCTGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGC‑GGGTTAA‑GGTAC‑‑TTCCCA‑CGCAC‑ACCAGACCGGCGGCGACATCCAGCGCCGACG‑CAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCG‑AACTCTTTTTCGTa                                                                                                                   <  1:142740/239‑1 (MQ=255)
                                                                                                                              cccTGCCTGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGC‑GGGTTAA‑GGTAC‑‑TTCCCA‑CGCAC‑ACCAGACCGGCGGCGACATCCAGCGCCGACG‑CAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCG‑AACTCTTTTTCGTaa                                                                                                                  <  1:205989/239‑1 (MQ=255)
                                                                                                                              cccTGCCTGGGCGCAGATAAGCAACGCTAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTCCATCTTCACTGATGGTTTCGTGGC‑GGTTTAA‑GGTAC‑‑TTCCCA‑CGCAC‑ACCAGACCGGCGGCGACATCCAGCGCCGACG‑CAAGGACCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCG‑AACTCTTTTTCGTaa                                                                                                                  <  1:622155/239‑1 (MQ=255)
                                                                                                                                          gcAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGC‑GGGTTAA‑GGTAC‑‑TTCCCA‑CGCAC‑ACCAGACCGGCGGCGACATCCAGCGCCGACG‑CAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCG‑AACTCTTTTTCGTAACGCTCCAGTTc                                                                                                       <  1:838776/238‑1 (MQ=255)
                                                                                                                                            aGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGTAAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGC‑GGGTTAA‑GGTAC‑‑TTCCCC‑CGCCC‑ACCAGACCGGCGGCGACATCCAGCGCCGACG‑CAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCG‑AACTCTTTTTCGTAACGCTCCAGTTCCa                                                                                                     <  1:1414220/238‑1 (MQ=255)
                                                                                                                                                    aaCGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGC‑GGGTTAA‑GGTAC‑‑TTCCCA‑CGCAC‑ACCAGACCGGCGGCGACATCCAGCGCCGACG‑CAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCG‑AACTCTTTTTCGTAACGCTCCAGTTCCATCCCCA‑ATg                                                                                           <  1:898163/239‑1 (MQ=255)
                                                                                                                                                      cgctAAGATCAATGGTCCCCATCCGCGATATCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGC‑GGGTTAA‑GGTAC‑‑TACCCG‑CGCAC‑ACCAGACCGGCGGCGACATCCAGCGCCGACG‑CAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCG‑AACTCTTTTTCGTAACGCTCCAGTTCCATCCCCA‑ATgcg                                                                                         <  1:124109/239‑1 (MQ=255)
                                                                                                                                                      cgcgAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGC‑GGGTTAA‑GGTAC‑‑TTCCCA‑CGCAC‑ACCAGACCGGCGGCGACATCCAGCGCCGACG‑CAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCG‑AACTCTTTTTCGTAACGCTCCAGTTCCATCCCCA‑ATgcg                                                                                         <  1:676535/239‑1 (MQ=255)
                                                                                                                                                      cgcgAAAATAAAGGGTCCCATCCCGCGATGGCCGCTGGCGTATTTCATCTTCACTGAGGGTTTCGTGGC‑GGGTTAA‑GGTAC‑‑TTCCCC‑CGCAC‑ACCAGCCCGGCGGCGACATCCAGCGCCGACG‑CAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCG‑AACTCTTTTTCGTAACGCTCCAGTTCCATCCCCA‑ATgcg                                                                                         <  2:712349/239‑1 (MQ=255)
                                                                                                                                                             aTCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGC‑GGGTTAA‑GGTAC‑‑TTCCCA‑CGCAC‑ACCAAACCGGCGGCGACATCCAGCGCCGACG‑CAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCG‑AACTCTTTTTCGTaa                                                                                                                  <  1:32312/208‑1 (MQ=255)
                                                                                                                                                              tCAATGGTCCCCATCCGCGATAGCCGCGGGCGTAGTTCATCTTCATTGATGGTTTCGTGGC‑GGGTTAA‑GGTAC‑‑TTCCCA‑CGCAC‑ACCAGACCGGCGGCGACATCCAGCGCCGACG‑CAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCG‑AACTCTTTTTCGTAACGCTCCAGTTCCATCCCCA‑ATGCGCGGTTa                                                                                   <  1:1323052/237‑1 (MQ=255)
                                                                                                                                                              tCAATGGACCCCGTCGGCTATAGCCGCTGGCGTAGTTCATCTTCACGGAGGGTTTCGTGGC‑GGGTAAA‑GGTAC‑‑TGCCCA‑CGCAC‑CCCAGGCCGGCGGCGACATCCAGCGCCGACG‑CAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCG‑AACTCTTTGTCGTAACGCTCCAGTTCCATCCCCA‑ATGCGCGGTTaaa                                                                                 <  2:416989/239‑1 (MQ=255)
                                                                                                                                                                  tGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGC‑GGGTTAA‑GGTAC‑‑TTCCCC‑CGCAC‑ACCAGACCGGCGGCGACATCCAGCGCCGACG‑CAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCG‑AACTCTTTTTCGTAACGCTCCAGTTCCATCCCCA‑ATGCGCGGTTAAATGGc                                                                             <  1:368967/239‑1 (MQ=255)
                                                                                                                                                                  tGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGC‑GGGTTAA‑GGTAC‑‑TTCCCA‑CGCAC‑ACCAGACCGGCGGCGACATCCAGCGCCGACG‑CAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCG‑AACTCTTTTTCGTAACGCTCCAGTTCCATCCCCA‑ATGCGCGGTTAAATGGc                                                                             <  1:885963/239‑1 (MQ=255)
                                                                                                                                                                  tGGTCCCCAGCCGCGATGGCCGCGGGCGTAGTTCATCTTCCCTGATGGTTTCGTGGC‑GGGTTAA‑GGTAC‑‑TTCCCA‑CGCAC‑ACCAGACCGGCGGCGACATCCAGCGCCGACG‑CAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCG‑AACTCTTTTTCGTAACGCTCCAGTTCCATCCCCA‑ATGCGCGGTTAAATGGc                                                                             <  1:719644/239‑1 (MQ=255)
                                                                                                                                                                     tCCCCATCCGCGTTAGCCGCTGGCGTAGTTCATCTTCACTGAGGGTTTCGTGGC‑GGGTTAA‑GGTAC‑‑TTCCCA‑CGCAC‑ACCGGACCGGCGGCGACATCCAGCGCCGACG‑CAATGCCCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCG‑AACTCTTTTTCGTAACGCTCCAGTTCCATCCCCA‑ATGCGCGGTTAAATGGCAt                                                                           <  1:469474/238‑1 (MQ=255)
                                                                                                                                                                     tCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGC‑GGGTTAA‑GGTAC‑‑TTCCCA‑CGCAC‑ACCAGACCGGCGGCGACATCCAGCGCCGACG‑CAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCG‑AACTCTTTTTCGt                                                                                                                    <  1:800272/198‑1 (MQ=255)
                                                                                                                                                                         cttCCGCGATAGCCGCTGGCGTGTTTCATCTTCACTGATGGTTTCGTGGC‑GGGTTAA‑GGTAC‑‑TTCCCA‑CGCAC‑ACCAGCCCGGCGGCGACATCCAGCGCCGCCG‑CAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCG‑AACTCTTTTTCGTAACGCTCCAGTTCCATCCCCA‑ATGCGCGGTTAAATGGCATGTGAt                                                                      <  2:1833/237‑1 (MQ=255)
                                                                                                                                                                         cATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGC‑GGGTTAA‑GGTAC‑‑TTCCCA‑CGCAC‑ACCAGACCGGCGGCGACATCCAGCGCCGACG‑CAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCG‑AACTCTTTTTCGTAACGCTCCAGTTCCATCCCCA‑ATGCGCGGTTAAATGGCATGTGAt                                                                      <  1:1107011/239‑1 (MQ=255)
                                                                                                                                                                         cATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGC‑GGGTTAA‑GGTAC‑‑TTCCCA‑CGCAC‑ACCAGACCGGCGGCGACATCCAGCGCCGACG‑CAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCG‑AACTCTTTTTCGTAACGCTCCAGTTCCATCCCCA‑ATGCGCGGTTAAATGGCATGTGAt                                                                      <  1:1060791/239‑1 (MQ=255)
                                                                                                                                                                              gcgATAGCAGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGC‑GGGTTAA‑GGTAC‑‑TCCCCA‑CGCCC‑ACCAGACCGGCGGCGACATCCAGCGCCGACG‑CAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCG‑AACTCTTTTTCGTAACGCTCCAGTTCCATCCCCA‑ATGCGCGGTTAAATGGCATGTGATaaaca                                                                 <  2:883721/239‑1 (MQ=255)
                                                                                                                                                                              gagATAGCCGCTTGCGTAGTTCATCTTCACTGATGGTTTCGTGGC‑GGGTAAA‑GGTAC‑‑TTCCCA‑CCCAC‑ACCAGACCGGCGGCGACATCCAGCGCCGACG‑CAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCG‑AACTCTTTTTCGTAACGCTCCAGTTCCATCCCCA‑ATGCGCGGTTAAATGGCATGTGATaaaca                                                                 <  2:1229400/237‑1 (MQ=255)
                                                                                                                                                                                  tAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGC‑GGGTTAA‑GGTAC‑‑TTCCCA‑CGCAC‑ACCAGACCGGCGGCGACATCCAGCGCCGACG‑CAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCG‑AACTCTTTTTCGTAACGCTCCAGTTCCATCCCCA‑ATGCGCGGTTAAATGGCATGTGATaaacaaac                                                              <  1:899283/238‑1 (MQ=255)
                                                                                                                                                                                         tGGCGTAGTTCATCTTCCCGGATGGTTTCGTGGC‑GGGTTAA‑GGTAC‑‑TTCCCC‑CGCAC‑CCCAGACCGGCGGCGACATCCAGCGCCGACG‑CAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCG‑AACTCTTTTTCGTAACGCTCCAGTTCCATCCCCA‑ATGCGCGGTTAAATGGCATGTGATAAACAAACATCTCAc                                                       <  2:612730/238‑1 (MQ=255)
                                                                                                                                                                                            cGTAGTTCATCTTCACTGATGGTTTCGTGGC‑GGGGTAA‑GGGGC‑‑TTCCCC‑CCCAC‑ACCCCACCGGCGGCGGCCACCCACGCCGCCC‑CCAAGAACCCCCCCCGCCAAAAACCTTTCGCCCCGCTTGCCCCCCACCATTTCCTGTTTTTTTAATGCC‑CCCGTGCGCAACCTTTTTTTTTAACCCCCCCTTTCCTCCCCCACATCCG‑GGTT‑AATTGCCTGTGATAAAAAAACATCTCAcccc                                                    >  2:563222/1‑237 (MQ=255)
                                                                                                                                                                                             gTAGTTCATCTTCACTGATGGTTTCGTGGC‑GGGTTAA‑GGTAC‑‑TTCCCA‑CGCCC‑ACCAGACCGGCGGCGACATCCAGCGCCGACG‑CAATGCCCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCG‑AACTCTTTTTCGTAACGCTCCAGTTCCATCCCCA‑ATGCGCGGTTAAATGGCATGTGATAAACAAACATCTCACCCACt                                                  <  1:385411/239‑1 (MQ=255)
                                                                                                                                                                                             gTAGTTCATCTTCACTGATGGTTTCGTGGC‑GGGTTAA‑GGTAC‑‑TTCCCA‑CGCAC‑ACCAGGCCGGCGGCGACATCCAGCGCCGACG‑CAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCG‑AACTCTTTTTCGTAACGCTCCAGTTCCATCCCCA‑ATGCGCGGTTAAATGGCATGTGATAAACAAACATCTCACCCACt                                                  <  1:1356571/239‑1 (MQ=255)
                                                                                                                                                                                                     tCTTCACTGATGGTTTCGTGGC‑GGGTTAA‑GGTAC‑‑TTCCCA‑CGCAC‑ACCAGACCGGCGGCGACATCCAGCGCCGACG‑CAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCG‑AACTCTTTTTCGTAACGCTCCAGTTCCATCCCCA‑ATGCGCGGTTAAATGGCATGTGATAAACAAACATCTCACCCACTAATTTCAg                                          <  1:544090/239‑1 (MQ=255)
                                                                                                                                                                                                        tCACTAAGGGTTTGGTGGC‑GGGTTAA‑GGTAC‑‑TTCCCA‑CGCAC‑CCCAGACCGGCGGCGACCTCCAGCGCCGCCG‑CAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCG‑AACTCTTTTTCGTAACGCTCCAGTTCCATCCCCA‑ATGCGCGGTTAAATGGCATGTGATAAACAAACATCTCACCCACTAATTTCAGGGc                                       <  1:263598/239‑1 (MQ=255)
                                                                                                                                                                                                               tGGTTTCGTGGC‑GGGTTAA‑GGTAC‑‑TTCCCA‑CGCAC‑ACCAGACCGGCGGCGACATCCAGCGCCGACG‑CAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCG‑AACTCTTTTTCGTAACGCTCCAGTTCCATCCCCA‑ATGCGCGGTTAAATGGCATGTGATAAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCa                                <  1:1179020/239‑1 (MQ=255)
                                                                                                                                                                                                               tGGTTTCGTGGC‑GGGTTAA‑GGTAC‑‑TTCCCA‑CGCAC‑ACCAGACCGGCGGCGACATCCAGCGCCGACG‑CAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCG‑AACTCTTTTTCGTAACGCTCCAGTTCCATCCCCA‑ATGCGCGGTTAAATGGCATGTGATAAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCa                                <  1:658351/239‑1 (MQ=255)
                                                                                                                                                                                                               tGGTTTCGGGGC‑GGGTTAA‑GGTGC‑‑TTCCCA‑CGCTC‑CCCAGACCGGCGGCGACATCCAGCGCCGACG‑CAATGCCCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCG‑AACTCTTTTTCGTAACGCTCCAGTTCCAGCCCCA‑ATGCGCGGTTAAATGGCATGTGATAAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCa                                <  1:1252320/239‑1 (MQ=255)
                                                                                                                                                                                                                ggTTGCGGGGC‑GGGGTAA‑GGTAC‑‑GTCCCA‑CGCAC‑ACCAAGCGGGCGGCGACATCCAGCGCCGACG‑CAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCAGCATTGGCTGATTTTTAAAGGCCAGCTGTGCG‑AACTCTTTTTCGTAACGCTCCAGTTCCATCCCCA‑ATGCGCGGTTAAATGGCATGTGATAAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCa                                <  2:249453/238‑1 (MQ=255)
                                                                                                                                                                                                                          c‑GGGTTAA‑GGTAC‑‑TTCCCA‑CGCAC‑ACCAGACCGGCGGCGACATCCAGCGCCGACG‑CAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCG‑AACTCTTTTTCGTAACGCTCCAGTTCCATCCCCA‑ATGCGCGGTTAAATGGCATGTGATAAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTa                      <  1:1093242/238‑1 (MQ=255)
                                                                                                                                                                                                                                tAA‑GGTAC‑‑TTCCCC‑CGCCC‑ACCAGACCGGCGGCCACATCCACCGCCAAAG‑CAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCG‑AACTCTTTTTCGTAACGCTCCAGTTCCATCCCCA‑ATGCGCGGTTAAATGGCATGTGATAAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACGGCAg                <  1:1182660/239‑1 (MQ=255)
                                                                                                                                                                                                                                tAA‑GGTAC‑‑TTCCCC‑CGCAC‑ACCAGACCGGCGGCGACATCCAGCGCCGACG‑CAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCG‑AACTCTTTTTCGTAACGCTCCAGTTCCATCCCCA‑ATGCGCGGTTAAATGGCATGTGATAAACAAACATCTCACCCACTAATTTCAGAGCTTGTTCAGCGGTCAGTACGGCa                 <  1:915233/238‑1 (MQ=255)
                                                                                                                                                                                                                                tAA‑GGTAC‑‑TTCCCA‑CGCTC‑ACCTGACCGGGGGCGACATCCTGGGCCGACG‑CAATGAGCCCGCCGTTCAAAATGTTTTGCGCCCAG‑TTGCTCACCATCATTGTCTGATTTTTAAAGGTCAGCTGTGCG‑AACTCTTTTTCGTAACGCTCCAGTTCCATCCCCA‑ATGCGCGGTTAAATGGCATGTGATAAACAAACATCTCACCCACTAATTTCAGCGCTTGTTCAGCGGTCACTACGGCa                 <  2:908169/238‑1 (MQ=255)
                                                                                                                                                                                                                                tAA‑GGTAC‑‑TTCCCA‑CGCAC‑ACCGGACCGGCGGCGACATCCAGCGCCGACG‑CAATGCCCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCG‑AACTCTTTTTCGTAACGCTCCAGTTCCATCCCCA‑ATGCGCGGTTAAATGGCATGTGATAAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACGGCa                 <  1:1244991/238‑1 (MQ=255)
                                                                                                                                                                                                                                tAA‑GGTAC‑‑TTCCCA‑CGCAC‑ACCAGACCGGCGGCGACATCCAGCGCCGACG‑CAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCG‑AACTCTTTTTCGTAACGCTCCAGTTCCATCCCCA‑ATGCGCGGTTAAATGGCATGTGATAAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACGGCa                 <  1:1025481/238‑1 (MQ=255)
                                                                                                                                                                                                                                tAA‑GGTAC‑‑TTCCCA‑CGCAC‑ACCAGACCGGCGGCGACATCCAGCGCCGACG‑CAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCG‑AACTCTTTTTCGTAACGCTCCAGTTCCATCCCCA‑ATGCGCGGTTAAATGGCATGTGATAAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACGGCa                 <  1:151305/238‑1 (MQ=255)
                                                                                                                                                                                                                                tAA‑GGTAC‑‑TTCCCA‑CGCAC‑ACCAGACCGGCGGCGACATCCAGCGCCGACG‑CAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCG‑AACTCTTTTTCGTAACGCTCCAGTTCCATCCCCA‑ATGCGCGGTTAAATGGCATGTGATAAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACGGCa                 <  1:969532/238‑1 (MQ=255)
                                                                                                                                                                                                                                tAA‑GGTAC‑‑TTCCCA‑CGCAC‑ACCAGACCGGCGGCGACATCCAGCGCCGACG‑CAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCG‑AACTCTTTTTCGTAACGCTCCAGTTCCATCCCCA‑ATGCGCGGTTAAATGGCATGTGATAAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACGGCa                 <  1:586069/238‑1 (MQ=255)
                                                                                                                                                                                                                                 aa‑GGTCC‑‑TGCCCC‑CGCAC‑ACCATTCCGGCGGCGTGATCCAGCTCCGTTG‑CAAGGACCCCGCCGTGCAAAATGCGTGTTGTCCTG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGTGGTGCT‑AACTCTTTTTCGTAACGCTCCAGTTCCATCCCCA‑ATGCGCGGTTAAATGGCATGTGATAAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTTTGTACGGCAGa               <  2:1079942/239‑1 (MQ=255)
                                                                                                                                                                                                                                  a‑GGTCC‑‑TTCCCC‑CGCAC‑ACCAAACCGGGGGCGACATCCACCGCCGCCG‑CAATGCCCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCG‑AACTCTTTTTCGTAACGCTCCAGTTCCATCCCCA‑ATGCGCGGTTAAATGGCATGTGATAAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACGGCAGAc              <  2:1216255/239‑1 (MQ=255)
                                                                                                                                                                                                                                   taaggcccgtCCCA‑CGCCC‑ACCCGACCGGCGGCGACAGCCAGCCCGGAGA‑CGTGGCCCCCGGCGTGCAAAATGCTTTGCGGCCAG‑TTGCCCACCATAAATGGCTGAAATATAAAGGCCAGCTGTGCG‑AGCTCTTTTTCGTAACTCTCCAGTGCCATCCCCA‑ATGCGCGGTTAAATGGCATGTGGGAAACAAACATCTCCCCCACTAATTTCACGGCTGGTTCAGCGGTGAGTACGGCa                 <  2:248431/229‑1 (MQ=255)
                                                                                                                                                                                                                                       aC‑‑TTCCCC‑CGCAC‑ACCAGACCGGCGGCGACATCCAGCGCCGACG‑CAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCG‑AACTCTTTTTCGTAACGCTCCAGTTCCATCCCCA‑ATGCGCGGTTAAATGGCATGTGATAAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACGGCAGACatat          <  1:117803/239‑1 (MQ=255)
                                                                                                                                                                                                                                       aC‑‑TTCCCA‑CGCAC‑ACCAGACCGGCGGCGACATCCAGCGCCGCCG‑CAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCG‑AACTCTTTTTCGTAACGCTCCAGTTCCATCCCCA‑ATGCGCGGTTAAATGGCATGTGATAAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACGGCAGACatat          <  2:732617/239‑1 (MQ=255)
                                                                                                                                                                                                                                       aC‑‑TTCCCA‑CGCAC‑ACCAGACCGGCGGCGACATCCAGCGCCGACG‑CAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCG‑AACTCTTTTTCGTAACGCTCCAGTTCCATCCCCA‑ATGCGCGGTTAAATGGCATGTGATAAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACGGCAGACatat          <  1:604715/239‑1 (MQ=255)
                                                                                                                                                                                                                                       aC‑‑TTCCCA‑CGCAC‑ACCAGACCGGCGGCGACATCCAGCGCCGACG‑CAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCG‑AACTCTTTTTCGTAACGCTCCAGTTCCATCCCCA‑ATGCGCGGTTAAATGGCATGTGATAAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACGGCAGACatat          <  1:230297/239‑1 (MQ=255)
                                                                                                                                                                                                                                       aC‑‑TTCCCA‑CGCAC‑ACCAGACCGGCGGCGACATCCAGCGCCGACG‑CAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCG‑AACTCTTTTTCGTAACGCTCCAGTTCCATCCCCA‑ATGCGCGGTTAAATGGCATGTGATAAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACGGCAGACatat          <  2:50341/239‑1 (MQ=255)
                                                                                                                                                                                                                                       aC‑‑TTCCCA‑CGCAC‑ACCAGACCGGCGGCGACATCCAGCGCCGACG‑CAATGACCCCGCCGTGCAAAATGCTGTGCGCCCAG‑GTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCG‑AACTCTTTGTCGTAACGCTCCAGTTCCATCCCCA‑ATGCGCGGTTAAATGGCATGTGATAAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACGGCAGACatat          <  1:670147/239‑1 (MQ=255)
                                                                                                                                                                                                                                       aC‑‑TTCCCA‑CGCAA‑ACCAGACCGGCGGCGACATCCAGCGCCGACG‑CAATGGCCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTTCCCAGCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCG‑AACTCTTTTTCGTAACGCTCCAGTTCCATCCCCA‑ATGCGCGGTTAAATGGCATGTGATAAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACGGCAGACatat          <  1:126441/239‑1 (MQ=255)
                                                                                                                                                                                                                                         cctccccA‑CGCAC‑CCCAGACCGGCGGCGACACCCAGCGCCGACG‑CAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCG‑AACTCTTTTTCGTAACGCTCCAGTTCCATCCCCA‑ATGCGCGGTTAAATGGCATGTGATAAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACGGCAGACatat          <  2:451275/235‑1 (MQ=255)
                                                                                                                                                                                                                                           ttCCCA‑CGCAC‑ACCAGACCGGCGGCGACATCCAGCGCCGACG‑CAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCG‑AACTCTTTTTCGTAACGCTCCAGTTCCATCCCCA‑ATGCGCGGTTAAATGGCATGTGATAAACAAACATCTCAc                                                       >  1:33975/1‑192 (MQ=255)
                                                                                                                                                                                                                                           ttCCCA‑CGCAC‑ACCAGACCGGCGGCGACATCCAGCGCCGACG‑CAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCG‑AACTCTTTTTCGTAACGCTCCAGTTCCATCCCCA‑ATGCGCGGTTAAATGGCATGTGATAAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACg                    <  1:611892/227‑1 (MQ=255)
                                                                                                                                                                                                                                           ttCCCA‑CGCAC‑ACCAGACCGGCGGCGACATCCAGCGCCGACG‑CAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCG‑AACTCTTTTTCGTAACGCTCCAGTTCCATCCCCA‑ATGCGCGGTTAAATGGCATGTGATAAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACg                    >  2:611892/1‑227 (MQ=255)
                                                                                                                                                                                                                                           ttCCCA‑CGCAC‑ACCAGACCGGCGGCGACATCCAGCGCCGACG‑CAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCG‑AACTCTTTTTCGTAACGCTCCAGTTCCATCCCCA‑ATGCGCGGTTAAATGGCATGTGATAAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACGGCa                 >  2:1390972/1‑230 (MQ=255)
                                                                                                                                                                                                                                           ttCCCA‑CGCAC‑ACCAGACCGGCGGCGACATCCAGCGCCGACG‑CAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCG‑AACTCTTTTTCGTAACGCTCCAGTTCCATCCCCA‑ATGCGCGGTTAAATGGCATGTGATAAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACGGCa                 <  1:1390972/230‑1 (MQ=255)
                                                                                                                                                                                                                                           ttCCCA‑CGCAC‑ACCAGACCGGCGGCGACATCCAGCGCCGACG‑CAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCG‑AACTCTTTTTCGTAACGCTCCAGTTCCATCCCCA‑ATGCGCGGTTAAATGGCATGTGATAAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACGGCAGACATatca        >  1:451625/1‑239 (MQ=255)
                                                                                                                                                                                                                                           ttCCCA‑CGCAC‑ACCAGACCGGCGGCGACATCCAGCGCCGACG‑CAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCG‑AACTCTTTTTCGTAACGCTCCAGTTCCATCCCCA‑ATGCGCGGTTAAATGGCATGTGATAAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACGGCAGACATatca        >  1:18989/1‑239 (MQ=255)
                                                                                                                                                                                                                                            tCCCA‑CGCAC‑ACCAGACCGGCGGCGACATCCAGCGCCGACG‑CAATGACCCCGCCGTGCAAAATGCTTTGCGCCAAG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCG‑AACTCTTTTTCGTAACGCTCCAGTTCCATCCCCA‑ATGCGCGGTTAAATGGCATGTGATAAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACGGCAGACATatca        >  2:1095945/1‑238 (MQ=255)
                                                                                                                                                                                                                                            tCCCA‑CGCAC‑ACCAGACCGGCGGCGACATCCAGCGCCGACG‑CAATGACCCCGCCGGGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCG‑AACTCTTTTTCGTAACGCTCCAGTTCCATCCCCA‑ATGCGCGGTTAAATGGCATTTGATAAACAAACATCACACCCACTACTTTTAGGGCTTGTTCAGCGGTCAGTACGGCAGACATatca        >  2:355952/1‑238 (MQ=255)
                                                                                                                                                                                                                                                  cGCAC‑ACCAGACCGGCGGCGACATCCAGCGCCGACG‑CAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCG‑AACTCTTTTTCGTAACGCTCCAGTTCCATCCCCA‑ATGCGCGGTTAAATGGCATGTGATAAACAAACATCTCACCCACTAATTTCAGGGCTTGTTTAGCGGTTAGTACGGCAGGCATATCATCCtt   >  2:1134778/1‑238 (MQ=255)
                                                                                                                                                                                                                                                  cGCAC‑ACCAGACCGGCGGCGACATCCAGCGCCGACG‑CAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCG‑AACTCTTTTTCGTAACGCTCCAGTTCCATCCCCA‑ATGCGCGGTTAAATGGCATGTGATAAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACGGCAGACATATCATCCTTa  <  1:306302/239‑1 (MQ=255)
                                                                                                                                                                                                                                                  cGCAC‑ACCAGACCGGCGGCGACATCCAGCGCCGACG‑CAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCG‑AACTCTTTTTCGTAACGCTCCAGTTCCATCCCCA‑ATGCGCGGTTAAATGGCATGTGATAAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACGGCAGACATATCATCCTTa  <  1:1323001/239‑1 (MQ=255)
                                                                                                                                                                                                                                                  cGCAC‑ACCAGACCGGCGGCGACATCCAGCGCCGACG‑CAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCG‑AACTCTTTTTCGTAACGCTCCAGTTCCATCCCCA‑ATGCGCGGTTAAATGGCATGTGATAAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACGGCAGACATATCATCCTTa  <  2:18989/239‑1 (MQ=255)
                                                                                                                                                                                                                                                  cGCAC‑ACCAGACCGGCGGCGACATCCAGCGCCGACG‑CAAGGACCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCG‑AACTCTTTTTCGTAACGCTCCAGTTCCATCCCCA‑ATGCGCGGTTAAATGGCATGTGATAAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACGGCAGACATATCATCCTTa  <  1:669770/239‑1 (MQ=255)
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                  
AACCTACCATATAGGTGGCGGTGGCACTGGCAATATAAAGCTGTTCTTCATTGTGTAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGC‑GGGTTAA‑GGTAC‑‑TTCCCA‑CGCAC‑ACCAGACCGGCGGCGACATCCAGCGCCGACG‑CAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAG‑TTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCG‑AACTCTTTTTCGTAACGCTCCAGTTCCATCCCCA‑ATGCGCGGTTAAATGGCATGTGATAAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACGGCAGACATATCATCCTTA  >  NC_000913/4004231‑4004706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A