Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F27 I0 R1	322	127.1	2757226	91.8%	2531133	223.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,005,348	A→C	20.7%	D163A (GAC→GCC)	pldA →	outer membrane phospholipase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,005,348	0	A	C	20.7%	150.4 / 6.5	80	D163A (GAC→GCC)	pldA	outer membrane phospholipase A
Reads supporting (aligned to +/- strand): ref base A (45/14); new base C (2/16); total (48/32)
Fisher's exact test for biased strand distribution p-value = 9.96e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01

AGGATGAAGTAAAGTTTCAGTTGAGCCTGGCATTTCCGCTGTGGCGTGGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGG‑CAACTGTCCAATAGCGAAGAGTCTTCA‑CCGTTTCG‑TGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTG‑CCACC‑GA‑TTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGA‑TGGGG‑TATAA‑CCACGACTCTAAC‑GGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACTGACGATAACCCGGATATCACCAAATATATGGGTTACTACCAGCTTAAAATCGGCTATCACCTCGGTGATGCGGTGCTCAGTGCGAAAGGACAGTACAACTGGAACACCGGCTACGGCGGC  >  NC_000913/4005111‑4005586

aGGATGAAGTAAAGTTTCAGTTGCGCCTGGCATTTCCGTTGGTGCGTGGGATTTTAGGCCCGTACTCGGTGTTGGGTTCGTCTTATACGCAAAAATCCTGGTGG‑CAACTGTCCAATAGCGAAGAGTCTTCA‑CCGTTTCG‑TGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTG‑CCACC‑GA‑TTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGA‑TGGGG‑TATAA‑CCACGa                                                                                                                                                                                                                                                 <  1:938145/238‑1 (MQ=255)

          aaaGTTTCAGTTGAGCCTGGCATTTCCGCTGTGGCGTGGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGG‑CAACTGTCCAATAGCGAAGAGTCTTCA‑CCGTTTCG‑TGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTG‑CCACC‑GA‑TTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGA‑TGGGG‑TATAA‑CCACGACTCTAAC‑gg                                                                                                                                                                                                                                       <  1:703481/237‑1 (MQ=255)

            aGTTTCAGTTGAGCCTGGCATTTCCGCTGTGGCGTGGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGG‑CAACTGTCCAATAGCGAAGAGTCTTCA‑CCGTTTCG‑TGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTG‑CCACC‑GA‑TTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGGGA‑TGGGG‑TATAA‑CCACGACTCTAAC‑CGGCGt                                                                                                                                                                                                                                   >  1:169422/1‑239 (MQ=255)

               ttCAGGTGAGCCTGGCATTTCCGTTGTGGCGTGGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTTG‑CAACTGTCCAATAGCGAAGAGTCTTCA‑CCGTTTCG‑TGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTG‑CCACC‑GA‑TTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGA‑TGGGG‑TATAA‑CCACGACTCTAAC‑GGGCGtt                                                                                                                                                                                                                                  <  1:386982/237‑1 (MQ=255)

                      gAGCCTGGCATTTCCGCTGTGGCGTGGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGG‑CAACTGTCCAATAGCGAAGAGTCTTCA‑CCGTTTCG‑TGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTG‑CCACC‑GA‑TTACCGTTTTGCAGGTTGGACGCTGCGCGATTTGGGGT‑TGGGG‑GATAA‑CCACGACTCTAAC‑GGGCGTTccgccccg                                                                                                                                                                                                                          >  1:515310/1‑238 (MQ=255)

                        gCCTGGCATTTCCGCTGTGGCGTGGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGG‑CAACTGTCCAATAGCGAAGAGGCTTCA‑CAGTTTCG‑TGAAACCACCTACGAACCGCAATTGCTCCTCGGTTTTG‑GCACC‑GC‑TTCCCGTTTTGACGGATGAGCGCTGCGCGATGTGGAGT‑GGGGG‑TATAA‑ACCCGGCCCAAAC‑GGGGGTTcccaaccacc                                                                                                                                                                                                                        >  2:84069/1‑230 (MQ=255)

                                   ccGCTGTGGCGTGGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGG‑CAACTGTCCAATAGCGAAGAGTCTTCA‑CCGTTTCG‑TGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTG‑CCACC‑GA‑TTACCGTTTTGCAGGTTGGACGCTGCGCGATTGGGAGA‑TGGGG‑TATAA‑CCACGCCCCAACA‑GGGCGTTCCGACCCGACCTCCCGTAGCTg                                                                                                                                                                                                            >  1:682155/1‑239 (MQ=255)

                                      cTGTGGCGTGGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGG‑CAACTGTCCAATAGCGAAGAGTCTTCA‑CCGTTTCG‑TGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTG‑CCACC‑GA‑TTACCGTTTTGCCGGTTGGACGCTCCGCGGTTTGGGGA‑TGGGG‑TATAA‑CCACGCCCCTAAC‑GGGCGTTcccacccggacccccgaaccggga                                                                                                                                                                                                          >  2:1375035/1‑222 (MQ=255)

                                       ttgggtggggttttttGGCCCCGACCTCGGTGTGGGGGGTGTTTTGTACACAAAAGCCCTGGGGG‑AACCTGCCAAAGAGGGAAGTGT‑TTCC‑CCCTGTGGTTAAAACCAACTACGAACCGAAATTGTTCCTCGGTTTTG‑CCACC‑GT‑TTACGGTTTTGCAGGTTGTACGGGGCGCGATGTGGAGA‑TGGGG‑TATAA‑CCACGACTCTAAC‑GGGCGTTCCGCCCCGACCTCCCGAGGCTGGAAc                                                                                                                                                                                                        <  2:512678/227‑1 (MQ=255)

                                       tggggggagggttttctggCCCGACCCGGGTGTTGGTGGTCTTTTATCCGCAAAAATCCGGGGGG‑CAACTGACAAAGAGCGAAGGTTCTTCC‑CCGTTTCG‑GTAAACCATCACCACCCCGGAATCGTCACTGTTTTCTG‑CCCCG‑TA‑TTCCCGTTTGGCAGGTTGGACGCTGCCGTATGTGGAGA‑TGGGG‑TCTAA‑CCGAGCCTCTAAC‑GGGCGTTCCGACCCGACCTCCCGAAGCTGGAAc                                                                                                                                                                                                        <  2:679389/222‑1 (MQ=255)

                                         tgGCGTGGGATTTTAGGCCCGAACTCGGTATTGGGTGCGTCTTATACGCAAAAATCCTGGTGG‑CAACTGTCCAATAGCGAAGAGTCTTCA‑CCGTTTCG‑TGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTG‑CCACC‑GA‑TTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGTGA‑TGGGG‑TATAA‑CCACGACTCTAAC‑GGGCGTTCCGCCCCGACCCCCCCCCGCGGGAccc                                                                                                                                                                                                       >  1:1341790/1‑238 (MQ=255)

                                           gCGTGGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGG‑CAACTGTCCAATAGCGAAGAGTCTTCA‑CCGTTTCG‑TGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTG‑CCACC‑GA‑TTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGA‑TGGGG‑TATAA‑CCACGACCCTAAA‑GGGCGTTCCGACCCGAACCCCCGCAGCTGGAACCg                                                                                                                                                                                                      >  2:230585/1‑237 (MQ=255)

                                           gCGTGGGATTTTAGGCCCGAACTCGGGGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGG‑CAACTGTCCAATAGCGAAGAGTCTTCA‑CCGTTTCG‑TGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTG‑CCACC‑GA‑TTAACGTTTTGCAGGTTGGACGCTGCGCGATGTGGGGA‑TGGGG‑TATAA‑CCACGACCCTAAC‑GGGCGTTCCGACCCGCGCTACCGCCGCTGGAAccc                                                                                                                                                                                                      >  1:910877/1‑236 (MQ=255)

                                              tGGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGG‑CAACTGTCCAATAGCGAAGAGTCTTCA‑CCGTTTCG‑TGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTG‑CCACC‑GA‑TTACCGTTTTGCAGGTTGGACGCTGCGCGAAGTGGGGA‑TGGGG‑TATAA‑CCACGACTCTAAC‑GGGCGTTCCGACCCGACCTCCCGCAGCCGGAACCGCCttt                                                                                                                                                                                                 >  1:157415/1‑239 (MQ=255)

                                                   ttttgggccggaccggtgttttGGGTGGGTTTTATCCG‑AAAAAACCTGGGGGAAAACGGTCAAATAGCGAAGGGTTTCAA‑CCGTTTCG‑TAAAACCAACTCCGAACCGCATTTGTTCCTCGGTTTTG‑CCCCC‑GA‑TTCCCGTTTGGCAGGTTGGACGCTGCGGGATGTGGAGG‑GGGGG‑TATAA‑CCCCGCCTCTAAC‑GGGCGTCCCGCCCCGACCTCCCGCAGCTGGAACCGCCTTTATACt                                                                                                                                                                                            <  2:468945/219‑1 (MQ=255)

                                                     ttAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGG‑CAACTGTCCAATAGCGAAGAGTCTTCA‑CCGTTTCG‑TGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTG‑CCACC‑GC‑TTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGA‑TGGGG‑GATAA‑CCACGACTCTAAC‑GGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATCCta                                                                                                                                                                                           >  1:301318/1‑237 (MQ=255)

                                                     ttAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGG‑CAACTGTCCAATAGCGAAGAGTCTTCA‑CCGTTTCG‑TGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTG‑CCACC‑GA‑TTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGA‑TGGGG‑TATAA‑CCACGACTCTAAC‑GGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTc                                                                                                                                                                                           >  1:253138/1‑238 (MQ=255)

                                                           ccGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGG‑CAACTGTCCAATAGCGAAGAGTCTTCA‑CCGTTTCG‑TGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTG‑CCACC‑GA‑TTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGA‑TGGGG‑TATAA‑CCACGACTCTAAC‑GGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCCCCCGAt                                                                                                                                                                                    >  1:1296411/1‑239 (MQ=255)

                                                             gAACTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGG‑CAACTGTCCAATAGCGAAGAGTCTTCA‑CCGTTTCG‑TGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTG‑CCACC‑GA‑TTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGA‑TGGGG‑TATAA‑CCACGACTCTAAC‑GGGCGTTCCGACCCGACCTCCCGCCGCTGGGAACCCCTTTATACTccccaggagg                                                                                                                                                                                  >  2:1291880/1‑233 (MQ=255)

                                                                         gggTGCGTCTTATACGCAAAAATCCTGGTGG‑CAACTGTCCAATAGCGAAGAGTCTTCA‑CCGTTTCG‑TGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTG‑CCACC‑GA‑TTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGA‑TGGGG‑TATAA‑CCACGACTCTAAC‑GGGCGGTCCGACCCGCCCTCCCGCAGCGGGAACCGCCTTTATACTCCCCCGCTGGCAAAAAACggga                                                                                                                                                                      >  2:1353824/1‑237 (MQ=255)

                                                                             gCGTCTTATACGCAAAAATCCTGGTGG‑CAACTGTCCAATAGCGAAGAGTCTTCA‑CCGTTTCG‑TGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTG‑CCACC‑GA‑TTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGA‑TGGGG‑TATAA‑CCACGACTCTAAC‑GGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGGAActg                                                                                                                                                                  >  1:178399/1‑239 (MQ=255)

                                                                               gTCTTATACGCAAAAATCCTGGTGG‑CAACTGTCCAATAGCGAAGAGTCTTCA‑CCGTTTCG‑TGAAACCAACTACGACCCGCAATTGTTCCTCGGTTTTG‑CCACC‑GA‑TTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGA‑TGGGG‑TATAA‑CCACGACCCTAAC‑GGGCGTTCCGACCCGACCTCCCGCAGCTGGAAACGCCTTTATACTCCTCTGATGGCAGAAACAGGTAActggt                                                                                                                                                                >  2:958892/1‑238 (MQ=255)

                                                                               gTCTTATACGCAAAAATCCTGGTGG‑CAACTGTCCAATAGCGAAGAGTCTTCA‑CCGTTTCG‑TGAAACCAACTACGACCAGCAATTGTTCTCCGGTTTTG‑CCACC‑GA‑TTACCGTTTTGCAGGTTGGACCCTGCGCGCTGTGGCGA‑TGGGG‑CATAA‑CCAAGACTCTAAC‑GGGCGTTCCGACCCCACCTTCCGCCGCTGGAACCGCCTTTATACCCCCCCGCTGCCCGAAAAAGGTAAatgag                                                                                                                                                                >  2:642628/1‑237 (MQ=255)

                                                                               gTCTTATACGCAAAAATCCTGGTGG‑CAACTGTCCAATAGCGAAGAGTCTTCA‑CCGTTTCG‑TGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTG‑CCACC‑GA‑TTACCGTTTTGCAGGCTGGCCGCTGCGCGATGTGGGGA‑TGGGG‑GTTAA‑CCACGACTCTAAC‑GGGCGTTCCGACCCGACCTCCCGCCGCTGGAACCCCCTTTATCCCCGCCTGATGGCCGAAAAAGGGaaaggtc                                                                                                                                                                >  2:754548/1‑234 (MQ=255)

                                                                                    ataCGCAAAAATCCTGGTGG‑CAACTGTCCAATAGCGAAGAGTCTTCA‑CCGTTTCG‑TGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTG‑CCACC‑GA‑TTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGA‑TGGGG‑TATAA‑CCACGACTCTAAC‑GGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTa                                                                                                                                                           >  1:37087/1‑239 (MQ=255)

                                                                                               acttggggg‑CATTTGTCCTAGAGCGAAGATTTTTAC‑CCGTTTGG‑AAAACCCAACTACGAACCGCAATTGTTCCACGTTTTTG‑CCCCC‑GA‑TTCCCGTTTTGCAGTTGGGCCGCGGGGGGATGTGGAGA‑TGGGT‑TATAA‑CCACGCCTCTAGC‑GGGCTTTCCGCCCCGACCTCCCGCAGCTGTAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGcc                                                                                                                                                <  2:150142/236‑1 (MQ=255)

                                                                                                cgggtggg‑CAACTGCCAAATGGGGAAGTTTCTTCC‑CCTTTTGG‑TAAAACCAACTACGAACCGCATTTGTTCCTCGTTTTTG‑CCCCC‑GT‑TTCCCTTTTGGCGGGTGGGACGCTGCGGGATGTGGGTA‑TGGGT‑TATAC‑CCACGACTCTAAG‑GGGCGTCCCGCCCCGCCCCCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGcc                                                                                                                                                <  2:1043661/232‑1 (MQ=255)

                                                                                                ccTGTTGG‑CAACTGTCCAATGGCGAAGGGTCTTCA‑CCGTTTCG‑TGAACCCAACTACGAACCGCAATTGTTCCTCGGTTTTG‑CCACC‑GA‑TTCCCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGA‑TGGGG‑TATAA‑CCACGACTCTAAC‑GGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCg                                                                                                                                               <  1:1279887/239‑1 (MQ=255)

                                                                                                 ttgtaaaaaacaaggaccaatgggAAGATTTTTCACCCTTTTCG‑GAAAACCAACTACGACCCGAAATTGTTCCCTGTTTTTG‑CC‑CC‑GATTTACCTTTTTGGAGGTTGGAGGCTGCGGTATGGGGAGA‑TGGGG‑TATAA‑CCACGCCTCTAAC‑GGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGcc                                                                                                                                                <  2:1127493/216‑1 (MQ=255)

                                                                                                 cctggggcaatcttcccAATAGCGAAGGTTTGTCC‑CCTTTTCG‑TAAACCAAACTACGACCCGCATTTGTTCTCGGGTTTTC‑CCCCC‑AT‑TTTCCTTTTGGCTGGTTGGACGCTGCGGGATGTGGAGA‑GGGGG‑TATAA‑CCACGCCTTTACC‑GGGCGTTCCGACCCGACCCCCCGCAGCGGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCg                                                                                                                                               <  2:682155/224‑1 (MQ=255)

                                                                                                    gtgg‑CAACTGTCCAATAGCGAAGAGTCTTCA‑CCGTTTCG‑TGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTG‑CCACC‑GA‑TTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGA‑TGGGG‑TATAA‑CCACGACTCTAAC‑GGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGt                                                                                                                                           >  1:545288/1‑239 (MQ=255)

                                                                                                         cAACTGTCCAATAGCGAAGAGTCTTCA‑CCGTTTCG‑TGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTG‑CCACC‑GA‑TTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGA‑TGGGG‑TATAA‑CCACGACTCTAAC‑GGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTAtgt                                                                                                                                       >  1:729213/1‑239 (MQ=255)

                                                                                                          ccctttCCAATGGGGAGGGGTCTTCC‑CCTTTTGG‑AAAACCCACCTTCGAGCGGAATTTTTTCCCGGTTTTTG‑CCCCG‑GT‑TTCCCTTTTTGCGGGTGGGACGCGGCGCGATGGGGAGA‑TGGGG‑TATAC‑CCACGCCTTTACC‑GGGCGTTCCGACCCGACCCCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTAtgtg                                                                                                                                      <  2:1233236/234‑1 (MQ=255)

                                                                                                          acttttcaaattggagggtgttttCC‑CCGTTTCT‑TGAACCAACCTCCGACCCGCAATTGTTCCTCGTTTGTG‑CCCCG‑GA‑TTCCCGTTTGGCGGGTTGGAGGTGGGGCGGTGTGGAGT‑GGGGT‑TATAC‑CCACGCCTCAAAC‑GGGCGTTCCGACCCGACCCCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTAtgtg                                                                                                                                      <  2:515310/217‑1 (MQ=255)

                                                                                                               tCCAATAGCGAAGAGTCTTCA‑CCGTTTCG‑TGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTG‑CCACC‑GA‑TTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGA‑TGGGG‑TATAA‑CCACGACTCTAAC‑GGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTAtgtg                                                                                                                                      >  1:1043661/1‑234 (MQ=255)

                                                                                                                       cGAAGAGTCTTCA‑CCGTTTCG‑TGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTG‑CCACC‑GA‑TTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGA‑TGGGG‑TATAA‑CCACGACTCTAAC‑GGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGAAATAccg                                                                                                                         >  1:553124/1‑237 (MQ=255)

                                                                                                                         cagagTCTTCA‑CCGTTTCG‑TGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTG‑CCACC‑GA‑TTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGA‑TGGGG‑TATAA‑CCACGACTCTAAC‑GGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCCCCTGATGGCAGAAAACGGTAACTGGCTGGGATAAGGTAAGCCGGGGTGAGTGGGGGGGaaaaaggaa                                                                                                                       >  2:573810/2‑232 (MQ=255)

                                                                                                                         aaGAGTCTTCA‑CCGTTTCG‑TGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTG‑CCACC‑GA‑TTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGA‑TGGGG‑TATAA‑CCACGACTCTAAC‑GGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTAtgtg                                                                                                                                      >  1:1127493/1‑224 (MQ=255)

                                                                                                                         aaGAGTCTTCA‑CCGTTTCG‑TGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTG‑CCACC‑GA‑TTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGA‑TGGGG‑TATAA‑CCACGACTCTAAC‑GGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGGGAAGCCGTGGTAGgtggctgtctcttttaca                                                                                                                       >  1:853669/1‑225 (MQ=255)

                                                                                                                         aaGAGTCTTCA‑CCGTTTCG‑TGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTG‑CCACC‑GA‑TTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGA‑TGGGG‑TATAA‑CCACGACTCTAAC‑GGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCGGGTAGAAGGGAAGCCGGGGGATGGGGGGGGGAAAACGGAc                                                                                                                       >  2:565244/1‑239 (MQ=255)

                                                                                                                              tCTTCA‑CCGTTTCG‑TGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTG‑CCACC‑GA‑TTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGA‑TGGGG‑TATAA‑CCACGACTCTAAC‑GGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATAATGACGATa                                                                                                                   >  1:297205/1‑238 (MQ=255)

                                                                                                                                         ttCG‑TGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTG‑CCACC‑GA‑TTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGA‑TGGGG‑TATAA‑CCACGACTCTAAC‑GGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTTGTCGAAGGGAAGCCGTGGGATGGGGTGGGTAATACTGACGATAACCCGGagata                                                                                                        >  1:598720/1‑238 (MQ=255)

                                                                                                                                             ttgaccccaccttggaatcgctgttGTTCCGTGTTTTTG‑CCCCT‑GA‑TTACCGTGTGGCTGGGTGGACGCTGC‑CGGAGGGGGGG‑TGGGG‑TTTAACCCCCGTCGCTAGC‑GGGCGTTCAGACCCGACCCCCCGCAGTGGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACTGACGATAACCCGGATATCAcc                                                                                                     <  2:218506/216‑1 (MQ=255)

                                                                                                                                             ttaaacccATCTACGACCTGCTTTTGTCCTGCGGTTTTG‑CCCCC‑GT‑TTCCGTTTTTGCAGTTTGGACGGTGGCGGATGGGGAGA‑TGGGA‑TAAAA‑CCCGGCCTCTACC‑GGGCGTTCCACCCCGCCCCCCGGCGGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACTGACGATAACCCGGATATCAcc                                                                                                     <  2:140610/233‑1 (MQ=255)

                                                                                                                                             tgcaacccAACTACGACCCCCATTTGTCCCGGGGTTGTG‑CCCAC‑GT‑TCGCTTTTTTGCGGTTTGGCGGCTGGGGGATGGGGAGA‑GGGGG‑GACAC‑CCCCGACT‑TAACGGGGTGTTCCGCCCCGACCCCCAGCAGGTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACTGACGATAACCCGGATATCAcc                                                                                                     <  2:727005/233‑1 (MQ=255)

                                                                                                                                             gttaaccccactccgaccccataTTGTTCCGGGTTTTTG‑CCACC‑GG‑TTACCGTTTTGCAGGTGGGCCGCGGCG‑GGTGTGGGGA‑GGGGGTTAAAA‑CCCCGACTTTAAC‑GGTGGTCCCGCCCCGCCCCCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACTGACGATAACCCGGATATCAcc                                                                                                     <  2:950673/217‑1 (MQ=255)

                                                                                                                                             gtgaaccccatgagaacccGAAATTGTTCCTGGGTTTTG‑CCCCG‑GT‑TTCCTGTTTGGCAGGTTGACCGCTGGGAGATGGGGAGA‑TGGGG‑TTAAC‑CCACGACTCAAAC‑GGGCGTTCCGACCGGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACTGACGATAACCCGGATATCAcc                                                                                                     <  1:565244/222‑1 (MQ=255)

                                                                                                                                                    ccccttccacccGCAGTTTTTCCGGGTTTTTG‑CCGCG‑GT‑GTACCGTTTGGAGGGTGGGGCGCTGCGGGATGTGGAGA‑GGGGT‑AATAC‑CCACGATTGTAAC‑GGGCGTCCGGACGCGCCCCACGGCACGGGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACTGACGATAACCCGGATATCACCAAatata                                                                                              <  2:972949/229‑1 (MQ=255)

                                                                                                                                                    cacttgaaacccGAAATTGTTCCGCGTTTGTG‑‑CACT‑GATTTACCTTTTGGCAGGTGGGCCGCTGCGCGATGTGGAGA‑GGGGG‑TAAAC‑CCAGGCTCCAAAC‑GGGGTTTCCGACCCGCCCCCCGGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACTGACGATAACCCGGATATCACCAAatata                                                                                              <  1:1375035/229‑1 (MQ=255)

                                                                                                                                                     gtcTACGACCCGTATGTTTTCCT‑GGTTTTGCCCACG‑GA‑TTACTGTTTGGGAGTTTGCACGCTGCGCTGTGTGAAGA‑TGGGG‑TTAAC‑CCACGACTCTAGC‑GGGGGTGCCGACCCGACCCCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACTGACGATAACCCGGATATCACCAAatatat                                                                                             <  2:1352484/237‑1 (MQ=255)

                                                                                                                                                                 cAATTGTTCCTCGGTTTTG‑CCACC‑GA‑TTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGA‑TGGGG‑TATAA‑CCACGACTCTAAC‑GGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACTGACGATAACCCGGATATCACCAAATATATGGGTtacaac                                                                                   >  1:1352484/1‑237 (MQ=255)

                                                                                                                                                                         ccTCGGTTTTG‑CCACC‑GA‑TTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGA‑TGGGG‑TATAA‑CCACGACTCTAAC‑GGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTCGAAGTGGAGCCGGGGTATGGGGTGGGGAATACTTAGGATAACCCGGATATACCCAAATATAGGGGGtacacacgactaaaaa                                                                         >  2:398139/1‑226 (MQ=255)

                                                                                                                                                                            ccgTTTTG‑CCCCCGGG‑TTCCCGTTGTGCTGGTGGGCGGCTGCG‑GGGGTGGAGAGGGGGG‑GAAAA‑CCCCGTCTCTAGC‑GGGCGTGCGGACCAGGCTCCCCGCGGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACTGACGATAACCCGGATATCACCAAATATATGGGTTACTACCAGCTTAAAATc                                                                       <  2:297205/237‑1 (MQ=255)

                                                                                                                                                                            cGGTTTTG‑CCACC‑GA‑TTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGA‑TGGGG‑TATAA‑CCACGACTCTAAC‑GGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACGGGCTGGTAGAAGTGAAGCCGTGGTATGTGGGGGGTAATACTGACGATAACCCGGGAATCACCAAATATATGGGTTAATACCAGCTTAAAATCg                                                                      >  2:965936/1‑239 (MQ=255)

                                                                                                                                                                                           gA‑TTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGA‑TGGGG‑TATAA‑CCACGACTCTAAC‑GGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGGGGTATGTGGTGGGAAATACTGCCGATAACCCGGCTATCACACAATATATGGGTTACGCCCCGCTTAAAATCGGCTACCAACCCgg                                                         >  2:1079637/1‑239 (MQ=255)

                                                                                                                                                                                            gtttccgttttgggtggTTGGGCGCTGGGGGGGGGGGAGA‑TGGGG‑TATAC‑CCCCGACTACAAC‑GGGGGTGCCGACCCGCCCTCCCGCGGCGGGACCCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACTGACGATAACCCGGATATCACCAAATATATGGGTTACTACCAGCTTAAAATCGGCTATCACCTCgg                                                         <  2:729213/224‑1 (MQ=255)

                                                                                                                                                                                              ttcctgTTTTGCAGGTGGGAGGCTGCGGGTGGTGGAGA‑GGGGG‑TATAC‑CCACGCCTCTAAC‑GGGCGTTCCGACCCGCCCTCCCGCAGCGGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACTGACGATAACCCGGATATCACCAAATATATGGGTTACTACCAGCTTAAAATCGGCTATCACCTCGGTg                                                       <  1:230585/234‑1 (MQ=255)

                                                                                                                                                                                                    ttttGCAGGTTGGACGCTGCGCGATGTGGAGA‑TGGGG‑TATAA‑CCACGACTCTAAC‑GGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACTGACGATAACCCGGATATCACCAAATATATGGGTTACTACCAGCTTAAAATCGGCTATCACCTCGGGGAAGCgg                                                 >  1:728899/1‑239 (MQ=255)

                                                                                                                                                                                                        gctggTTGGCCGCGGCGCGATGTGGAGA‑TGGGG‑TATAA‑CCACGCCTCTAAC‑GGGCTGTCCGACCCGACATCCCCCAGATGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACTGACGATAACCCGGATATCACCAAATATATGGGTTACTACCAGCTTAAAATCGGCTATCACCTCGGTGATGCGGTGCt                                             <  1:687368/236‑1 (MQ=255)

                                                                                                                                                                                                        gcgggTTGGGCGCGGCGCGATGGGGTGA‑TGGGT‑TATAA‑CCACGACTTTAAG‑GGGCGTTCCTACCCGACCTCCCGCAGCGGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACTGACGATAACCCGGATATCACCAAATATATGGGTTACTACCAGCTTAAAATCGGCTATCACCTCGGTGATGCGGTGCt                                             <  1:754548/236‑1 (MQ=255)

                                                                                                                                                                                                          aGGTTGGACGCTGGTGGATGGGGAGA‑TGGGG‑TATAC‑CCACGACTCTAAG‑GGGCGTCCCGACCCGACCTCCCGCAGCGGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACTGACGATAACCCGGATATCACCAAATATATGGGTTACTACTAGCTTAAAATCGGCTATCACCTCGGTGATGCGGTGCTc                                            <  1:75069/238‑1 (MQ=255)

                                                                                                                                                                                                           gtttggccccttgtcggtgtgGAGT‑TGGGG‑TAATC‑CCCCTCCTCTAGC‑GGGCGTTCCGACCCCACCTCCCGCAGCTGAACCCGCTTTTATACTCGCCTGATGGGAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACTGACGATAACCCGGATATCACCAAATATATGGGTTACTACCAGCTTAAAATCGGCTATCACCTCGGTGATGCGGTGCTCAg                                          <  2:253138/222‑1 (MQ=255)

                                                                                                                                                                                                              tGGACGCTGCGCGATGTGGAGA‑TGGGG‑TATAA‑CCACGACTCTAAC‑GGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTggg                                                                                                                                 >  1:578011/1‑149 (MQ=255)

                                                                                                                                                                                                                 ccGCTGCGCGTGGGGGAGT‑TGGGG‑TATAC‑CCACGCTCCTAGC‑GGGCGTTCCGACCCGACCCCCCGCAGCGGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACTGACGATAACCCGGATATCACCAAATATATGGGTTACTACCAGCTTAAAATCGGCTATCACCTCGGTGATGCGGTGCTCAGTGCGa                                     <  2:178399/237‑1 (MQ=255)

                                                                                                                                                                                                                           tttggggaagtGGGT‑TATAA‑CCACGCCCCTAAC‑GGGCGTTCCGACCCGACCCCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACTGACGATAACCCGGATATCACCAAATATATGGGTTACTACCAGCTTAAAATCGGCTATCACCTCGGTGATGCGGTGCTCAGTGCGAAAGGACAGt                            <  1:573810/228‑1 (MQ=255)

                                                                                                                                                                                                                            tgtgGAGA‑GGGGG‑TATAA‑CCACGCCTCTAAC‑GGGCGTCCAGCCCCGCCCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGaa                                                                                                                                                   <  2:1301358/117‑1 (MQ=255)

                                                                                                                                                                                                                                      gggg‑TATAC‑CCAGTCCTTTACC‑GGGCGTTCCGACCCGACCTCCCGCAGCGGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACTGACGATAACCCGGATATCACCAAATATATGGGTTACTACCAGCTTAAAATCGGCTATCACCTCGGTGATGCGGTGCTCAGTGCGAAAGGACAGTACAACTGGAac                 <  1:1009266/238‑1 (MQ=255)

                                                                                                                                                                                                                                       ggg‑TTTCA‑CCCCGACCTTAAC‑TGGCTCTCCGACCCGCCCTCCAGCAGTGGGAACCCCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTAGGTGGTGGGTAATACTGACGATAACCCGGATATCACCAAATATATGGGTTACTACCAGCTTAAAATCGGCTATCACCTCGGTGATGCGGTGCTCAGTGCGAAAGGACAGTACAACTGGAaca                <  2:1166685/238‑1 (MQ=255)

                                                                                                                                                                                                                                       ggg‑TATAA‑CCACGACTCTAAC‑GGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGt                                                                                                                                                      >  1:1287053/1‑104 (MQ=255)

                                                                                                                                                                                                                                       ggg‑TATAA‑CCACGACTCTAAC‑GGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTAt                                                                                                                                         >  1:1301358/1‑117 (MQ=255)

                                                                                                                                                                                                                                       ggg‑TATAA‑CCACGACTCTAAC‑GGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACTGACGCTAACCCGGGTATCCACAAATATATGGGTTACTACCAGCTTAAAATCGGCTATCACCTCGGGGGTACGGTGCTCAAAGCGaaaaaaaagaaaaactgaaaaac               >  2:665143/1‑219 (MQ=255)

                                                                                                                                                                                                                                       ggg‑TATAA‑CCACGACTCTAAC‑GGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACTGACGATAACCCGGATATCACCAAATATATGGGTTACTACCAGCTTAAAATCGGCTATCACCTCGGTGATGCGGTGCTCAGTGCGAAAGGACAGTACAACTGGAaca                >  1:549700/1‑238 (MQ=255)

                                                                                                                                                                                                                                       ggg‑TATAA‑CCACGACTCTAAC‑GGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACTGACGATAACCCGGATATCACCAAATATATGGGTTACTACCAGCTTAAAATCGGCTATCACCTCGGTGATGCGGTGCTCAGTGCGAAAGGACAGTACAACTGGAaca                >  1:1046955/1‑238 (MQ=255)

                                                                                                                                                                                                                                         g‑TATAA‑CCACGACTCTAAC‑GGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGCCAGAAAACAGTAACTGGCGGGGAAAAGGGAAGCCGTGGTACGTGGTGGGTAATATTGCCGAAAACCCGGAAGTCACACAATAACTGGGTTACTACCCGCTTAAAATCAGCTATCACCCCGGTGATCCGGTGCTCAGTGCGAAAGGaaaagacacaaagaaaaccg             >  2:361837/1‑220 (MQ=255)

                                                                                                                                                                                                                                               a‑CCACGACTCTAAC‑GGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACTGACGATAACCCGGATATCACCAAATATATGGGTTACTACCAGCTTAAAATCGGCTATCACCTCGGTGATGCGGTGCTCAGTGCGAAAGGACAGAAAAAATGGAAAACCGGCAAc        >  2:274157/1‑239 (MQ=255)

                                                                                                                                                                                                                                                    cGACTCTAAC‑GGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACTGACGCTAACCCGGATATCACCAAATATATGGGGTACTACCAGCTTAAAATCGCCTATAACCTCGGTGATGCGGTGTTCCGTGCGAAAAGa                                >  2:812820/1‑211 (MQ=255)

                                                                                                                                                                                                                                                    cGACTCTAAC‑GGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACTGACGATAACCCGGATATCACCAAATATATGGGTTACTACCAGCTTAAAATCGGCTATCACCTCGGTGATGCGGTGCTCAGTGCGAAAGGCCAGTACAACTGGAACACCGGCTAcgccg    >  1:27344/1‑239 (MQ=255)

                                                                                                                                                                                                                                                    cGACTCTAAC‑GGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACTGACGATAACCCGGATATCACCAAATATATGGGTTACTACCAGCTTAAAATCGGCTATCACCTCGGTGATGCGGTGCTCAGTGCGAAAGGACAGTCCAACTGGAACACCGGCTAcggcg    >  1:708530/1‑239 (MQ=255)

                                                                                                                                                                                                                                                    cGACTCTAAC‑GGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACTGACGATAACCCGGATATCACCAAATATATGGGTTACTACCAGCTTAAAATCGGCCATCACCTCGGTGATGCGGGGCCCAGTGCGAAAGGCCAGAACAACTGGAAAACCGGCCAcgggg    >  2:842034/1‑237 (MQ=255)

                                                                                                                                                                                                                                                    cGACTCTAAC‑GGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGAGGTGGGTAATACTGACGATAACCCGGATATCACCAAATATATGGGCTACTACCAGCTTACAATCGGCTATCACCTCGGTGATGCGGTGCTCAGTGGGAAAGGACAGTACAACTGGAACACCGGCTAcgccg    >  1:356440/1‑239 (MQ=255)

                                                                                                                                                                                                                                                     gACTCTAAC‑GGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATTGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACTGACGATAACCCGGATATCACCAAATATATGGGTTACTACCGGCTTAAAATCGGCTATCACCCCGGTGATACGGGGCTCAGTGCGAAAGGAAAGGACAAATGGACCACCgacgccggacg   >  2:381565/1‑229 (MQ=255)

                                                                                                                                                                                                                                                      aCTCTAAC‑GGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACTGACGATAACCCGGATATCACCAAATATATGGGTTACTACCAGCTTAAAATCGGCTATCACCACGGTGATGCGGTGCTAAATGCGAAAAGAAAGAACAAATGGAAAACCGGCAAcgggggg  >  2:680064/1‑238 (MQ=255)

                                                                                                                                                                                                                                                      aCTCTAAC‑GGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACTGACGATAACCCGGATATCACCAAAGATATGGGTTACTACCAGCTTAAAATCGGCTATCACCTCGGTGATGCCGTGCTAAGTGCGAAAGGACAGTACCACTGGAACACCGGCAACggcggc  >  2:1045714/1‑239 (MQ=255)

AGGATGAAGTAAAGTTTCAGTTGAGCCTGGCATTTCCGCTGTGGCGTGGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGG‑CAACTGTCCAATAGCGAAGAGTCTTCA‑CCGTTTCG‑TGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTG‑CCACC‑GA‑TTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGA‑TGGGG‑TATAA‑CCACGACTCTAAC‑GGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACTGACGATAACCCGGATATCACCAAATATATGGGTTACTACCAGCTTAAAATCGGCTATCACCTCGGTGATGCGGTGCTCAGTGCGAAAGGACAGTACAACTGGAACACCGGCTACGGCGGC  >  NC_000913/4005111‑4005586

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A