Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A15 F18 I0 R1 55 72.5 2455354 98.2% 2411157 146.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,241,196 Δ1 bp 6.1% coding (65/663 nt) yqjA → general envelope maintenance protein; DedA family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,241,1950C. 6.1% 195.9 / 9.7 66coding (64/663 nt)yqjAgeneral envelope maintenance protein; DedA family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (29/32);  new base . (2/2);  total (31/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.27e-01

AGAACATACCAAAACAGCAATAACATTGCGGTAGTGCATCTTTTAAAACCAGCGTGGCGTTAACCGATTCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTGGTATTGGTCGGCGTGTTGATTGCGAAAGGCGCGATGGGCTATCCGCAAA  >  CP009273/3241048‑3241342
                                                                                                                                                   |                                                                                                                                                    
aGAACATACCAAAACAGCAATAACATTGCGGTAGTGCATCTTTTAAAACCAGCGTGGCGTTAACCGATTCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATcc                                                                                                                                                     <  1:1270476/149‑1 (MQ=255)
  aaCATACCAAAACAGCAATAACATTGCGGTAGTGCATCTTTTAAAACCAGCGTGGCGTTAACCGATTCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCAt                                                                                                                                                   <  1:801476/149‑1 (MQ=255)
      tACCAAAACAGCAATAACATTGCGGTAGTGCATCTTTTAAAACCAGCGTGGCGTTAACCGATTCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCgat                                                                                                                                               <  1:28226/149‑1 (MQ=255)
         cAAAACAGCAATAACATTGCGGTAGTGCATCTTTTAAAACCATCGTGGCGTTAACCGATTCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATc                                                                                                                                                  <  2:312593/143‑1 (MQ=255)
          aaaaCAGCAATAACATTGCGGTAGTGCATCTTTTAAAACCAGCGTGGCGTTAACCGATTCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GAtt                                                                                                                                          <  1:219643/149‑1 (MQ=255)
                gCAATAACATTGCGGTAGTGCATCTTTTAAAACCAGCGTGGCGTTAACCGATTCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAAT‑CATCGATGGATTGGccag                                                                                                                                    <  1:1104103/149‑4 (MQ=255)
                 cAATAACATTGCGGTAGTGCATCTTTTAAAACCAGCGTGGCGTTAACCGATTCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGCGTTTGAAACCCTCGCCAAGCCATCGAT‑GATTg                                                                                                                                         >  2:126128/1‑143 (MQ=255)
                   aTAACATTGCGGTAGTGCATCTTTTAAAACCAGCGTGGCGTTAACCGATTCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCAtgttg                                                                                                                                 >  1:415980/1‑149 (MQ=255)
                    tAACATTGCGGTAGTGCATCTTTTAAAACCAGCGTGGCGTTAACCGATTCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAACCCATCGCT‑GGCTGGCa                                                                                                                                      >  2:266549/1‑143 (MQ=255)
                    tAACATTGCGGTAGTGCATCTTTTAAAACCAGCGTGGCGTTAACCGATTCACCAGGAATAATGAATGGAACTTTTGACCAAATTAATGCAATTACTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGcg                                                                                                                                      >  2:680098/1‑142 (MQ=255)
                    tAACACTGCGTAAGTGCATCTTTTAAAACCAGCGTGGCGTTAACCGCTTCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGAGTTTGAAACCCTGGCCAAT‑CATCGATGGATTGGccagggtt                                                                                                                                <  1:262577/149‑8 (MQ=255)
                     aaCATTGCGGTAGTGCATCTTTTAAAACCAGCGTGGCGTTAACCGATTCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTa                                                                                                                               <  1:817703/149‑1 (MQ=255)
                                    cATCTTTTAAAACCAGCGTGGCGTTAACCGATTCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCtt                                                                                                                      >  2:937338/1‑143 (MQ=255)
                                     aTCTTTTAAAACCAGCGTGGCGTTAACCGATTCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCACAGGGTTTTGAAACCCTGGCCAATCCCTCGAT‑GATTGGCAGGTTGTATTTTGTCttg                                                                                                                     >  2:892725/1‑143 (MQ=255)
                                     aTCTTTTAAAACCAGCGTGGCGTTAACCGATTCACCAGGAATAATGAATGGAACTTTGGACCCAATTGCTGCAAGCCCTGTGTGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GAGTGGCATGTTGGATTTTGTCTTGTTTGTa                                                                                                               >  1:276320/1‑149 (MQ=255)
                                                ccAGCGTGGCGTTAACCGATTCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAAtttt                                                                                                          <  2:791684/143‑1 (MQ=255)
                                                    cGTAGCGTTAACCGATTCACCAGGAATAATGAATGTAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGaa                                                                                                <  1:124939/149‑1 (MQ=255)
                                                       ggCGTTAACCGATTCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCtt                                                                                                   <  2:948675/143‑1 (MQ=255)
                                                         cGTTAACCGATTCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGa                                                                                                 >  2:876163/1‑143 (MQ=255)
                                                         cGTTAACCAATTCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGa                                                                                                 <  2:45955/143‑1 (MQ=255)
                                                             aaCCTATCCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCACTCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGgcttg                                                                                       <  1:1240454/149‑1 (MQ=255)
                                                             aaCCGATTCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAAc                                                                                             >  2:254076/1‑143 (MQ=255)
                                                                  aTTCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGCCTTGTTTGTAATTTTGTTCCTTGAAAACGgctt                                                                                        >  2:441684/1‑143 (MQ=255)
                                                                    tCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTCTTCCTTGAAAACGGCTTGCTTCcgg                                                                                <  1:652752/149‑1 (MQ=255)
                                                                      aCCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCcggcg                                                                              >  1:543298/1‑149 (MQ=255)
                                                                        cAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCggcggc                                                                            <  1:299306/149‑1 (MQ=255)
                                                                             ataatGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAGAACGGCTTGCTTCCGGCGGCCtttt                                                                       <  1:775516/149‑1 (MQ=255)
                                                                             ataatGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCtttt                                                                       >  1:1243831/1‑149 (MQ=255)
                                                                             ataatGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGATTTcggcgt                                                                             >  2:704511/1‑142 (MQ=255)
                                                                              taatGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTCGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTTTTTGTAATTTTGTTCCTTCAAAACGGCTTGCTTCCGGCGGCCttttt                                                                      <  1:732018/149‑1 (MQ=255)
                                                                                atgactgGAACTTTTGACCCAATCGGTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTTATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTAc                                                                    <  1:256748/149‑1 (MQ=255)
                                                                                atgaatgGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAATGGCTTGCTTCCGGCGGCCTTTTTAc                                                                    <  1:727214/149‑1 (MQ=255)
                                                                                atgaatgGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTAc                                                                    >  1:1038004/1‑149 (MQ=255)
                                                                                    atgGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCAGCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCggg                                                                <  1:3005/149‑1 (MQ=255)
                                                                                     tgGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGc                                                               <  1:252103/149‑1 (MQ=255)
                                                                                            tttGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTCCCGGGCg                                                              >  2:523705/1‑143 (MQ=255)
                                                                                                  ccAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTgg                                                  >  1:870137/1‑149 (MQ=255)
                                                                                                     aTTGCTGCAAGCCCTATGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTAc                                                     >  2:623461/1‑143 (MQ=255)
                                                                                                       tgctgcCCGCCCTGTGGGCGCAGTATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTGGTATTg                                             <  1:1266797/149‑1 (MQ=255)
                                                                                                        gctgcAAGCCCTGTGGGCGCATGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATTTTGTATTTTGTCTTGTTTGTAATTTTGTTCATTGTATTCTGCGGGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTGGTATTgg                                            <  1:255771/149‑1 (MQ=255)
                                                                                                         ctgcACGTCCTGTGGGCGCAGGATTTTGAAGCCCTGCCCAAGCCATCGAC‑TATTGGCAGGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTGGTATTGGt                                           <  1:93830/149‑1 (MQ=255)
                                                                                                         ctgcAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGTCGGCCTTTTTACCGGGCGACAGTTTACTGGTATTGGt                                           <  1:842151/149‑1 (MQ=255)
                                                                                                            cAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGCCGACAGTTTACTGGTCtt                                              >  2:569114/1‑143 (MQ=255)
                                                                                                             aaGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTGGTATTg                                             <  2:998518/143‑1 (MQ=255)
                                                                                                              aGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTGGTATTGGTCGGCg                                      <  1:1206355/149‑1 (MQ=255)
                                                                                                                cgcTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTGGTATTGGTc                                          >  2:300588/3‑143 (MQ=255)
                                                                                                                cccTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGCCAGTTTACTGGTATTGGTCGGCgtg                                    >  1:277137/1‑149 (MQ=255)
                                                                                                                       ggCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTGGTATTGGTCGGCGTGTTGATTg                             <  1:999317/149‑1 (MQ=255)
                                                                                                                         agccgcaTTTTGAAACCCTGGTCAATCCATCGGT‑GATTGGCATGTTGTTTTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGGTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTGGTATTGGTCGGCGTGTTg                                 <  2:1085899/137‑1 (MQ=255)
                                                                                                                          gcAGGATTTTGAAACCCTGGCCAATGCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTGGTATTGGTCGGCGTGTTGATTGCGa                          <  1:1073559/149‑1 (MQ=255)
                                                                                                                            aGGATTTTGAAACCCTGGCCAATCCATTGAT‑GAGTGGACTGTTGTATTTTGTTTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCCGCGGCCTTTTTACCGGGCGACAGTTTACTGGTATTGGTCGGCGTGTTGATTGCGaaa                        <  1:428134/149‑1 (MQ=255)
                                                                                                                              gATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTGGTATTGGTCGGCGTGTTGATTGCGAAAgg                      >  1:1024270/1‑149 (MQ=255)
                                                                                                                              gATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTGGTATTGGTCGGCGTGTTGATTGCGAAAgg                      >  1:107930/1‑149 (MQ=255)
                                                                                                                                ttttGAACCCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTGGTATTGGTCGGCGTGTTGATTGCGa                          <  2:638651/143‑1 (MQ=255)
                                                                                                                                ttttGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTGGTATTGGTCGGCGTGTTGATTGCGa                          >  2:29098/1‑143 (MQ=255)
                                                                                                                                   tGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTGGTATTGGTCGGCGTGTTGATTGCGACAGGCGCGa                 >  1:1214831/1‑149 (MQ=255)
                                                                                                                                   tGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTGGTATTGGTCGGCGTGTTGATTGCGAAAGGCGCGa                 <  1:1162319/149‑1 (MQ=255)
                                                                                                                                      aaCCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAACACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTGGTATTGGTCGGCGTGTTGATTGCGAAAGGCGCGATgg              >  1:789076/1‑149 (MQ=255)
                                                                                                                                       caacatgCCAAT‑CATCGATGGATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTGGTATTGGTCGGCGTGTTGATTGCGAAAGGCGCGATggg             >  1:168682/7‑149 (MQ=255)
                                                                                                                                       caacatgCCAAT‑CATCGATGGATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTGGTATTGGTCGGCGTGTTGATTGCGAAAGGCGCGATggg             >  1:979955/7‑149 (MQ=255)
                                                                                                                                          cTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTGGTATTGGTCGGCGTGTTGATTGCGAAAGGCGCGATGGGCTa          <  1:674898/149‑1 (MQ=255)
                                                                                                                                            ggCCAATCCATCGAT‑GATTGGCAGGTTGTATTTTGTCTTGGTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTGGTATTGGTCGGCGTGTTGCTTGCGAAAGGCGCGATgg              >  2:1100406/1‑143 (MQ=255)
                                                                                                                                             gCCAATCCATCGGT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTGGTATTGGTCGGCGTGTTGATTGCGAAAGGCGCGATggg             <  2:41342/143‑1 (MQ=255)
                                                                                                                                               cAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTTAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTGGTATTGGTCGGCGTGTTGATTGCGAAAGGCGCGATGGGCTATCCGc     >  1:575177/1‑149 (MQ=255)
                                                                                                                                                aaTCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACCGTTTATTGGTTTGGGTCGGCGTGTTGATTGCTGAAGGCGCGATGGGCTATCCGCa    >  1:298229/1‑149 (MQ=255)
                                                                                                                                                aaTCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTGGTATTGGTCGGCGTGTTGATTGCGAAAGGCGCGATGGGCTATCCGCa    >  1:258387/1‑149 (MQ=255)
                                                                                                                                                  tCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTGGTATTGGTCGGCGTGTTGATTGCGAAAGGCGCGATGGGCTATCCGCaaa  <  1:550914/149‑1 (MQ=255)
                                                                                                                                                   ccATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTGGTATTGGTCGGCGTGTTGATTGCGAAAGGCGCGATGGGCTATcc       >  2:293229/1‑143 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
AGAACATACCAAAACAGCAATAACATTGCGGTAGTGCATCTTTTAAAACCAGCGTGGCGTTAACCGATTCACCAGGAATAATGAATGGAACTTTTGACCCAATTGCTGCAAGCCCTGTGGGCGCAGGATTTTGAAACCCTGGCCAATCCATCGAT‑GATTGGCATGTTGTATTTTGTCTTGTTTGTAATTTTGTTCCTTGAAAACGGCTTGCTTCCGGCGGCCTTTTTACCGGGCGACAGTTTACTGGTATTGGTCGGCGTGTTGATTGCGAAAGGCGCGATGGGCTATCCGCAAA  >  CP009273/3241048‑3241342

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A