Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A16 F20 I0 R1
|
52 |
51.1 |
1736300 |
96.9% |
1682474 |
149.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
CP009273 |
3,960,789 |
C→G |
18.3% |
T338R (ACA→AGA) |
rho → |
transcription termination factor |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | CP009273 | 3,960,789 | 0 | C | G | 18.3%
| 93.4
/ 20.6
| 44 | T338R (ACA→AGA) | rho | transcription termination factor |
| Reads supporting (aligned to +/- strand): ref base C (14/22); new base G (5/3); total (19/25) |
| Fisher's exact test for biased strand distribution p-value = 2.62e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.95e-01 |
TGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATT > CP009273/3960642‑3960937
|
tgGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACa < 2:148872/149‑1 (MQ=255)
gggATGCCACCGCCCTGTATCTTCCGAAACGCTGCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCGTATCGATACCGGTTTTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACa < 2:208100/148‑1 (MQ=255)
ggATGCCAACGCCCTGCATCGTCCGCAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATTGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGTAGTTATCTACGAAGAGTTTAAAGGTACAg < 2:532863/149‑1 (MQ=255)
aTGCCACCGCCCGGCAGCGTCCGAAACGCTTCTTTGGTGCGGCGCTTAACGTGGACGAGGGCGGCAGCCTGACTATTATCGTGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGc < 2:534088/149‑1 (MQ=255)
gCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTACCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTAGAGGCaa < 2:337534/149‑1 (MQ=255)
aaCGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGGCGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCGACAt > 2:663831/1‑149 (MQ=255)
aCGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGCGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTCCGAATAGTTTAAAGGTACAGGCAACATg < 2:523098/149‑1 (MQ=255)
gCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTAGAGGCAACATGGAACTGc < 2:75459/149‑1 (MQ=255)
cATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCa > 2:105561/1‑149 (MQ=255)
tCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCAcc < 1:263997/149‑1 (MQ=255)
cGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTCGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAACGTACAGGCAACATGGAACTGCACct < 2:485752/149‑1 (MQ=255)
gcgtcttTGGTGCTGCGCGTAAAGTGGAGGAGGGCGGCAGCATGACCATTATTGCGACGGCGATTACCGATACCGGGTGTAAAATGGACGAAGTTATATACGAAGAGTTTAAAGGTACAGGCAGAATGGAACTGCGCATCTCTCGaaac > 1:301854/4‑148 (MQ=255)
cttcttTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGa < 2:617508/149‑1 (MQ=255)
ttcttTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTAGAGGCAACATGGAACTGCACCTCTCTCGTAAGAt < 1:699324/149‑1 (MQ=255)
ttcttTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGAt < 2:350108/149‑1 (MQ=255)
tcgttGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATGATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATc < 1:756058/146‑1 (MQ=255)
tGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGaaa < 2:760717/149‑1 (MQ=255)
cgcgTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCTGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAAcgc < 1:624097/149‑1 (MQ=255)
tAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCt > 2:819171/1‑149 (MQ=255)
tGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATGCCGGTTCTAAAATGGACGAAGTTGTCTACGAGGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTGAGATCGCTGAAAAACGCGTATTccag > 2:680962/1‑147 (MQ=255)
tGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGCGTTTAAAGGTACAGGCACCCTGGACCTGCCCCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCg > 2:350110/1‑149 (MQ=255)
agagGGCGGCAGCCTGACCATTATCGCGACGGCGATTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTa > 2:441170/1‑149 (MQ=255)
gcggcgGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGAc < 2:101098/149‑1 (MQ=255)
gcggcAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGAc > 2:805053/1‑149 (MQ=255)
ggcggcCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTa < 1:391150/149‑1 (MQ=255)
aGCTTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACaa < 2:217370/149‑1 (MQ=255)
ccTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACACGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAAcc > 2:588769/1‑149 (MQ=255)
cATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGAAAAAAAAAAAAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTg < 1:482390/149‑1 (MQ=255)
cggtACCGGTACTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTAGAGGCAACATGGAACTGCACACCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTACCGACTACAACCGTTCTGGTACCCGTAAAGACGAgctgc > 1:799803/4‑149 (MQ=255)
cGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTAGAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAACAAGAgctgc > 1:722370/1‑149 (MQ=255)
gATACCGGTTCTAAAATGGGCGAAGGTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAgctgct < 1:39747/149‑1 (MQ=255)
gATACCGGTTCTAAAATGGACGAAGTTATATACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAgctgct < 1:552081/149‑1 (MQ=255)
cGGTTCTAAAATGGACGAAGTTATCTAAGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGa < 2:478541/149‑1 (MQ=255)
gggcGAAGTTATCTACGCAGAGTTTAAAGGTACAGGCAACATGGAGCTGCACCTCTCTCGTAAGAACACAGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAAc < 1:805053/146‑1 (MQ=255)
gACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACt > 1:475651/1‑149 (MQ=255)
aCGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTg > 2:496730/1‑149 (MQ=255)
aGTTATCTACGAAGAGTTTAAAGGTAGAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGGCTTCCCGGCTAGCGACTACAACCGTTCTGGTACCCGTAAAGAACAGCTGCTCACGACGCAGGAAGAACGACAGa > 2:557662/1‑149 (MQ=255)
cTACGAAGCTTTTAAATGCACAGGCAACATACCACCGCACCTCCCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAAtgt < 2:162457/149‑1 (MQ=255)
tACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAAtgtg < 1:105561/149‑1 (MQ=255)
aCGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGGACTGCAGAAAATGTgg > 1:37717/1‑149 (MQ=255)
aCGAAGAGCTTAAAGGTAGAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCCGGAAGAACTGCAGAAAACGTgg > 1:211090/1‑149 (MQ=255)
aaGAGTTTAAAGGTAGAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAAAGCGTCTTCCCGGCTAGCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGGAGAACTGCAGAAAATGTGGATc > 2:334949/1‑149 (MQ=255)
tttAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTgcg > 1:408765/1‑149 (MQ=255)
tAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAAAGCGTCTTACAGGCTATCGAATACAACTGTCCCGTGACCCGTAAAGAAGAACTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCa > 2:854050/1‑149 (MQ=255)
cAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCAtt < 2:475651/149‑1 (MQ=255)
|
TGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATT > CP009273/3960642‑3960937
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
