Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A17 F172 I0 R1	42	33.2	716278	96.8%	693357	139.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	2,360,799	A→G	100%	G66G (GGT→GGC)	adk ←	adenylate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,360,799	0	A	G	100.0%	99.1 / ‑6.5	37	G66G (GGT→GGC)	adk	adenylate kinase
Reads supporting (aligned to +/- strand): ref base A (2/0); new base G (16/18); total (19/18)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TGATGACAGCATCAATGTTTCTGTCAAGCTCAGACATAATATTATTTAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATACCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTGAACATGTCACCAGTTGAAATGTGGGGTATTGGGAATTTCTTGA  >  CP000730/2360663‑2360935

tgatgaCAGCATTAATGTTTCTGTCAAGCTCAGAGATTATATTATTTAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCt                                                                                                                                       <  2:275788/140‑1 (MQ=255)

 gatgaCAGCATCAATGTTTACCTGCACCTCAGACATAATATTATTTAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTa                                                                                                                                      >  1:354306/1‑140 (MQ=255)

        gCATCAATGTTTCTGTCAAGCTCAGACATAATATTATTTAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCaa                                                                                                                               >  1:294733/1‑139 (MQ=255)

          aTCAATGTTTCTGTCAAGCTCAGACATAATATTATTTAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCGACAGTCACtt                                                                                                                             <  2:159111/140‑1 (MQ=255)

           tCAATGTTTCTGTCAAGCTCAGACATAATATTATTTAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTtc                                                                                                                            <  1:287847/140‑1 (MQ=255)

              aTGTTTCTGTCAAGCTCAGACATAATATTATTTAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCCCTtcatc                                                                                                                         >  2:337762/1‑140 (MQ=255)

                    cTGTCAAGCTCAGACATAATATTATTTAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATGTAATAAAAAGCCTTTTTTTGGATGGTCTTCAGAACTTCTTTCTTTAACGATTCTTACAGTCACTTAATGAgggca                                                                                                                   >  2:83327/1‑137 (MQ=255)

                          atcTCAGACATAACATTATTTAATGCCTCAGCTTGCTCGATTGTTCTGGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTc                                                                                                             <  1:333108/138‑1 (MQ=255)

                          aGCTCAGACATAATATTATTTAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAACTTCTTCACTTAACAAGACCTACAGTCACTTCATCAGGAACTAATTc                                                                                                             >  1:274745/1‑140 (MQ=255)

                              cAGACATAATATTATTTAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGccc                                                                                                         <  2:255090/140‑2 (MQ=255)

                              cAGACATAATATTATTTAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATAAGGAACTAATTCGCCa                                                                                                         <  1:176578/140‑1 (MQ=255)

                                  cATAATATTATTTAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTAATCAGGAACTAATTCGCCACGGt                                                                                                     >  1:20397/1‑140 (MQ=255)

                                      atatTATTTAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCTTACAGTCACTTCATCAGGAACTAATTAGCAAAGGTCCat                                                                                                 >  1:109533/1‑140 (MQ=255)

                                              tAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACt                                                                                         <  1:277768/140‑1 (MQ=255)

                                               aaTGCCGCAGCTTGCATGGTTGGTCTTGGGAAGCCATCGAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATGCTTTCCGTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACtt                                                                                        <  1:312849/140‑1 (MQ=255)

                                                     tCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTGGACACGGTCCATATAAGAATTAGctta                                                                                  >  2:103655/1‑139 (MQ=255)

                                                     tCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCCCGGCCCAAATAAGACTTAGcttc                                                                                  >  1:92979/1‑140 (MQ=255)

                                                           tGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTAcc                                                                            <  1:42125/140‑1 (MQ=255)

                                                           tGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCGTCAGTAATTCTTTCCTTAACGATGCCTACACTCCCTTCATCAGGAACTAATTCGCCACGGTCCATATAATACTAAGCTTCTTTAcc                                                                            >  2:37711/1‑140 (MQ=255)

                                                            gCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGAAATTAACAAACACGTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTCCCt                                                                           >  1:350686/1‑140 (MQ=255)

                                                                 aTTGTTCTTGGGAAGCCATCTGATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTc                                                                      <  1:71921/140‑1 (MQ=255)

                                                                  ttGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCa                                                                     >  2:269244/1‑140 (MQ=255)

                                                                      tCTTGGGAAGCCATCTAATAAAGATCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTAAAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGttt                                                                 <  1:95951/140‑1 (MQ=255)

                                                                       cTTGGGAAGCCATCTAATAATAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTAAGTttc                                                                >  1:225298/1‑140 (MQ=255)

                                                                       cTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTttc                                                                >  1:251570/1‑140 (MQ=255)

                                                                              aGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTTAGTTTCTTCtttt                                                         >  1:7760/1‑140 (MQ=255)

                                                                               gCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTta                                                        <  2:319116/140‑1 (MQ=255)

                                                                                            aaGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTGaa                                           >  2:59948/1‑140 (MQ=255)

                                                                                                  ttttttGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTAATAGCTTTTCTGCACATgcc                                     >  1:106451/1‑138 (MQ=255)

                                                                                                        gCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTGAACATGTCACCCGt                               >  1:357109/1‑140 (MQ=255)

                                                                                                          aTCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATTCATATGCTGAACATGTCACCAGTTg                             >  2:249016/1‑140 (MQ=255)

                                                                                                                 tCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTCATTCGCCCCGGTCCATATAAGACTTCGCTTCTTTACCTATTTCGGTTTCTTCTTTTATAGCTTTTCTGAACATGTCACCAGTTGAAAtgtg                      <  1:70800/140‑1 (MQ=255)

                                                                                                                 tCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTGAACATGTCACCAGTTGAAAtgtg                      <  2:320218/140‑1 (MQ=255)

                                                                                                                 tCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATCCAGTTTCTTCTTTTATAGCTTTTCTGAACATGTCCCCAGTTGAAAtgtc                      <  2:314110/140‑2 (MQ=255)

                                                                                                                             gtcTTATCGAGGCCGACAGTCACTTCATCTGGACCTAATTTGCCACGGTCCATATAACACTTAGCTTCTTTACCTCATTCCGTTTCTTCTTTAATAGCTTTTCTGAACATGTCACCAGTTGAAATGTGGGGTATTGGGaa          <  2:87639/138‑1 (MQ=255)

                                                                                                                             gctttAACGATGCCTACAGTCTCTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTGAACATCGCTCTAGTTGAGATGTGGGGTATTGGGaa          <  2:148083/137‑1 (MQ=255)

                                                                                                                                     gATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTGAACATGTCACCAGTTGAAATGTGGGGTATTGGGAATTTCTTGa  <  1:341207/140‑1 (MQ=255)

TGATGACAGCATCAATGTTTCTGTCAAGCTCAGACATAATATTATTTAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATACCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTGAACATGTCACCAGTTGAAATGTGGGGTATTGGGAATTTCTTGA  >  CP000730/2360663‑2360935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A