Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F110 I0 R1	50	71.1	1529928	95.4%	1459551	140.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,761,889	C→A	100%	D388Y (GAT→TAT)	folC ←	tetrahydrofolate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,761,889	0	C	A	100.0%	188.4 / ‑6.5	60	D388Y (GAT→TAT)	folC	tetrahydrofolate synthase
Reads supporting (aligned to +/- strand): ref base C (1/0); new base A (34/25); total (35/25)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTCTTCATGGCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTAATCGGT  >  CP000730/1761752‑1762022

ttcttcATGGCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTTGTCATatac                                                                                                                                    >  2:117883/1‑141 (MQ=255)

  cttcATGGCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATatacta                                                                                                                                  <  1:63125/141‑1 (MQ=255)

      aTGGCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTg                                                                                                                              <  1:621963/141‑1 (MQ=255)

      aTGGCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAACTTTTTATAAATTCAACGTAGACATATACAAATTg                                                                                                                              >  1:602106/1‑141 (MQ=255)

        ggCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATCCTAATTGtt                                                                                                                            >  2:53870/1‑140 (MQ=255)

        ggCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTa                                                                                                                            >  1:333602/1‑141 (MQ=255)

           tATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTAttt                                                                                                                         <  2:182105/141‑1 (MQ=255)

                tagttttagtttTATAATGCTTCATAGTCTGATTTTGATTTAACTTCACTTGTGAAATACAGACTACCGGTAATTACTAATGTATAACCTTGATAATTTTTTATAAATTCAAAGTAGACATATACTAATTGTATttcttct                                                                                                                    >  2:539111/1‑140 (MQ=255)

                   ttttagttttaTAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTAATAAttt                                                                                                                 >  2:74953/1‑141 (MQ=255)

                           ttaTAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTTAACGTAGTCATATACTAATTGTATTTCATCATTTTCAAtact                                                                                                         >  2:446296/1‑141 (MQ=255)

                           ttaTAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTtcataagtgaagttaaa                                                                                                         <  2:330946/141‑14 (MQ=255)

                              taAGGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACATACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGCATTTCATCATTTTCAATActacc                                                                                                      <  1:129389/141‑1 (MQ=255)

                               aaTGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATActacct                                                                                                     <  2:92034/141‑1 (MQ=255)

                                       aaaGTATTATTTTCATTTAACTTCCCTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTCCAATACTACCTACAATttc                                                                                             <  1:401395/141‑1 (MQ=255)

                                           tCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATAAACTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTtcttct                                                                                         >  2:719033/1‑141 (MQ=255)

                                           tCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGCTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTTCTCATTTTCAATACTACCTACAATTttttct                                                                                         >  2:35142/1‑141 (MQ=255)

                                             tAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCttt                                                                                       >  2:52920/1‑141 (MQ=255)

                                             tAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCttt                                                                                       >  2:285967/1‑141 (MQ=255)

                                              aaTTTTGATTTAACTTCACTTATGAAATACCGACTACCGGTAATTACTAATGTATCACCTAGATCATTTTTTATAAATTCAACGTAGTCATCTACTAATTTTCTTTCATCCTTTACCATACTTCCTCCAATTTCCTCtttt                                                                                      >  2:291512/1‑140 (MQ=255)

                                               aTTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGc                                                                                     >  1:135641/1‑141 (MQ=255)

                                                 tttGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTCATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGt                                                                                   <  1:146077/141‑1 (MQ=255)

                                                 tttGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGt                                                                                   >  2:165595/1‑141 (MQ=255)

                                                 tttGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGt                                                                                   <  1:117286/141‑1 (MQ=255)

                                                 tttGATCTAATTTCTCTTATTAAATACTGAATACCGTCAATTATATATGTATTACTTTGATACTTTTTTATAAATTCAACGTAGTCATATACTACTTGTATTTCATCATTTTTAATACTATCTACATTTTCTTCTTTGCGt                                                                                   <  2:280486/141‑1 (MQ=255)

                                                      tttAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACgt                                                                              >  2:6084/1‑140 (MQ=255)

                                                       ttAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCGTCATTTTCAATACTACCTACAATTTCTTCGTTGCGTaaggtt                                                                             >  2:602523/1‑137 (MQ=255)

                                                        tAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCtt                                                                            >  2:176397/1‑141 (MQ=255)

                                                            ttaaCTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTCTTTCATCATTTTCAATACTATCGTCAATTTCTTCTTTGCGTAACGCTTTCgg                                                                        <  1:366363/138‑1 (MQ=255)

                                                            ttCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATATTTTCTACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCgg                                                                        >  2:276505/1‑141 (MQ=255)

                                                                cTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGaaaa                                                                    <  1:264047/141‑1 (MQ=255)

                                                                  tATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATc                                                                  <  2:35679/141‑1 (MQ=255)

                                                                   aTGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCa                                                                 <  1:54398/141‑1 (MQ=255)

                                                                        aTACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTc                                                            >  2:13809/1‑141 (MQ=255)

                                                                             gACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTg                                                       >  1:138796/1‑141 (MQ=255)

                                                                              aCTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGc                                                      >  2:144997/1‑141 (MQ=255)

                                                                              aCTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGc                                                      <  1:243359/141‑1 (MQ=255)

                                                                                 aCCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATa                                                   >  2:428899/1‑141 (MQ=255)

                                                                                  ccGGTAATTACTAATGTATCACCTTGATAATTTTTTATTAATTCTACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTACCCTTTTCGGAAAATCACATTCAGTTCCATaa                                                  >  2:687894/1‑141 (MQ=255)

                                                                                   cGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATaaa                                                 <  1:160792/141‑1 (MQ=255)

                                                                                           aCTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATgc                                         <  1:165595/141‑1 (MQ=255)

                                                                                                tGTATCACCTTGATAATTTTTTATAAATTCAACGTTGTCATATTCTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAAt                                    <  2:214035/141‑1 (MQ=255)

                                                                                                   aTCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACGATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTaa                                 >  1:495208/1‑141 (MQ=255)

                                                                                                            aTAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATg                        >  2:224114/1‑141 (MQ=255)

                                                                                                            aTAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATg                        >  2:128056/1‑141 (MQ=255)

                                                                                                             tAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAACTTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGt                       >  1:121597/1‑141 (MQ=255)

                                                                                                                 tttttATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTCAATGTTTGa                   >  1:421840/1‑141 (MQ=255)

                                                                                                                 tttttATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGa                   <  1:77918/141‑1 (MQ=255)

                                                                                                                 tttttATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGa                   >  1:109696/1‑141 (MQ=255)

                                                                                                                 tttttATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGa                   >  1:603916/1‑141 (MQ=255)

                                                                                                                  ttttATAAATTCAACTTAGTCATATACTAATGGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGAc                  <  1:446296/141‑1 (MQ=255)

                                                                                                                      tcaaaTTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCAtc              <  2:623817/139‑1 (MQ=255)

                                                                                                                      ataAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCAtc              <  1:719033/141‑1 (MQ=255)

                                                                                                                      ataAATTCAACGTAGTCATATACTAATTGTATTGCATCATTTTCAATACTACCTACAATTTCCTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCAtc              <  1:35142/141‑1 (MQ=255)

                                                                                                                       taAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCAtct             >  1:2531/1‑141 (MQ=255)

                                                                                                                         aaTTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCg           >  2:617672/1‑141 (MQ=255)

                                                                                                                            tCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTa        <  1:276505/141‑1 (MQ=255)

                                                                                                                               aCGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTAATc     >  1:2901/1‑141 (MQ=255)

                                                                                                                                cGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTAATCg    >  2:761159/1‑141 (MQ=255)

                                                                                                                                cGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTGCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTAATCg    >  1:503360/1‑141 (MQ=255)

                                                                                                                                cGTAATCATATACTAATTGTATTTCATCATATTCAATACTACCTACAATTTCTCCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATTTTTCACCATCTCGTTAAtgt    >  2:462695/1‑139 (MQ=255)

                                                                                                                                 gTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTAATCgg   <  1:176397/141‑1 (MQ=255)

                                                                                                                                  tAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTAATCGGt  <  1:13809/141‑1 (MQ=255)

TTCTTCATGGCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTAATCGGT  >  CP000730/1761752‑1762022

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A