breseq  version 0.32.1  revision 5f2cb669173e
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 39,378 C→A 100% D586Y (GAT→TAT)  mecA ← penicillin binding protein 2 prime
RA 76,969 G→A 100% intergenic (+1027/‑107) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 110,651 C→T 7.9% D170D (GAC→GAT USA300HOU_0109 → staphylococcal tandem lipoprotein
RA 110,660 T→A 14.3% I173I (ATT→ATA USA300HOU_0109 → staphylococcal tandem lipoprotein
RA 111,512 A→G 30.8% S194S (TCA→TCG USA300HOU_0110 → possible staphylococcal tandem lipoprotein
RA 111,516 C→A 31.3% Q196K (CAA→AAA)  USA300HOU_0110 → possible staphylococcal tandem lipoprotein
RA 111,560 T→C 10.4% R210R (CGT→CGC USA300HOU_0110 → possible staphylococcal tandem lipoprotein
RA 195,286 T→A 100% intergenic (‑97/‑82) USA300HOU_0183 ← / → USA300HOU_0184 hypothetical protein/hypothetical protein
RA 217,384 A→G 7.1% M344T (ATG→ACG)  USA300HOU_0199 ← possible LIVCS family branched chain amino acid:cation symporter
RA 228,319 T→C 6.8% intergenic (+165/‑523) USA300HOU_0207 → / → hsdR2 RpiR family transcriptional regulator/type I site‑specific deoxyribonuclease restriction subunit
RA 310,609 A→G 4.8% intergenic (‑54/‑95) USA300HOU_0275 ← / → USA300HOU_0276 GntR family transcriptional regulator/PTS family glucose/glucoside (glc) porter component IIABC
RA 396,540 G→A 100% A208T (GCA→ACA)  USA300HOU_0364 → possible lipoprotein
RA 495,289 G→A 7.7% Q277* (CAG→TAG)  USA300HOU_0470 ← hypothetical membrane protein
RA 512,643 A→G 48.4% intergenic (+711/‑150) recR → / → rrs1 recombination protein RecR/16S ribosomal RNA
RA 607,884 G→A 100% R209* (CGA→TGA)  USA300HOU_0550 ← deoxynucleoside kinase
RA 629,681 C→A 100% L84I (CTA→ATA)  USA300HOU_0564 → possible 3‑hexulose‑6‑phosphate synthase
RA 665,392 T→G 100% L79* (TTA→TGA)  USA300HOU_0608 → possible acetytransferase
RA 678,888 G→T 11.2% intergenic (+91/+160) USA300HOU_0621 → / ← sarA hydrolase/staphylococcal accessory regulator A
RA 707,910 A→G 100% intergenic (‑1/‑121) USA300HOU_0653 ← / → USA300HOU_0654 ABC superfamily ATP binding cassette transporter, ABC protein/possible metal‑dependent transcriptional regulator
RA 744,511 G→C 5.1% intergenic (+2348/‑578) USA300HOU_0687 → / → sarX hypothetical membrane protein/possible staphylococcal accessory protein X
RA 826,969 G→T 6.3% A36D (GCT→GAT)  USA300HOU_0775 ← hypothetical membrane protein
RA 1,154,017 C→A 8.5% A181E (GCA→GAA)  USA300HOU_1079 → MutS family DNA mismatch repair protein
RA 1,233,504 T→A 100% V470D (GTT→GAT)  USA300HOU_1157 → non‑specific serine/threonine protein kinase
RA 1,243,307 A→T 5.0% S249C (AGT→TGT)  USA300HOU_1165 → fatty acid/phospholipid synthesis protein
RA 1,274,208 C→T 100% S204L (TCA→TTA)  USA300HOU_1186 → CodY family transcriptional regulator
RA 1,416,458 A→T 100% I216N (ATT→AAT)  USA300HOU_1314 ← oligopeptide ABC superfamily ATP binding cassette transporter, ABC protein
RA 1,440,085 G→T 100% intergenic (‑45/‑891) cspA ← / → xpaC cold shock protein CspA/5‑bromo‑4‑chloroindolyl phosphate hydrolysis protein XpaC
RA 1,513,930 A→T 100% L413F (TTA→TTT USA300HOU_1379 → hypothetical membrane protein
RA 1,646,189 G→C 100% A155G (GCA→GGA)  USA300HOU_1531 ← M24B family Xaa‑Pro dipeptidase
RA 1,685,149 T→A 5.3% intergenic (‑161/+59) USA300HOU_1575 ← / ← rpsU hypothetical membrane protein/ribosomal protein S21
RA 1,744,362 G→A 100% H104Y (CAC→TAC)  apt ← adenine phosphoribosyltransferase
RA 1,761,889 C→A 100% D388Y (GAT→TAT)  folC ← tetrahydrofolate synthase
RA 1,857,867 C→T 9.0% L169L (TTG→TTA USA300HOU_1728 ← hypothetical protein
RA 1,909,490:1 +T 100% coding (297/303 nt) USA300HOU_1771 → hypothetical membrane protein
RA 1,963,768 C→G 100% V365L (GTA→CTA)  hemG ← protoporphyrinogen oxidase
RA 2,010,363 C→T 100% C146Y (TGT→TAT)  USA300HOU_1861 ← ABC superfamily ATP binding cassette transporter, membrane protein
RA 2,028,269 G→C 100% P126A (CCA→GCA)  USA300HOU_1882 ← hypothetical membrane protein
RA 2,077,013 T→C 9.8% Y86C (TAT→TGT)  USA300HOU_1933 ← hypothetical membrane protein
RA 2,171,944 A→G 6.8% N509D (AAT→GAT)  leuA2 → 2‑isopropylmalate synthase
RA 2,211,990 C→T 5.9% V196I (GTC→ATC)  USA300HOU_2087 ← hypothetical membrane protein
RA 2,235,173 C→G 8.3% A185A (GCG→GCC tdk ← thymidine kinase
RA 2,279,705 G→A 100% intergenic (+1373/+6659) mtlD → / ← glmM mannitol‑1‑phosphate 5‑dehydrogenase/phosphoglucosamine mutase
RA 2,352,815 G→A 100% A92V (GCA→GTA)  rpsI ← ribosomal protein S9
RA 2,371,220 C→T 17.1% intergenic (‑4337/+1221) rpmC ← / ← USA300HOU_2234 ribosomal protein L29/NCS2 family nucleobase:cation symporter‑2
RA 2,384,414 T→C 7.3% Y217C (TAT→TGT)  fmhB ← peptidoglycan pentaglycine interpeptide biosynthesis protein FmhB
RA 2,454,626 A→T 100% L48L (CTA→CTT USA300HOU_2315 → hypothetical membrane protein
RA 2,532,298 A→T 100% T15T (ACA→ACT USA300HOU_2392 → FemAB family peptidoglycan biosynthesis protein
RA 2,632,292 C→A 15.6% intergenic (‑83/+599) fnbB ← / ← fnbA fibronectin‑binding protein B/fibronectin‑binding protein A
RA 2,632,296 G→A 14.2% intergenic (‑87/+595) fnbB ← / ← fnbA fibronectin‑binding protein B/fibronectin‑binding protein A
RA 2,816,469 G→C 15.2% Y620* (TAC→TAG USA300HOU_2654 ← serine‑threonine rich protein

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP000730 512620 =73 (1.120)54 (1.200)
+40 bp
40/180 NT 51.7% intergenic (+688/‑173) recR/rrs1 recombination protein RecR/16S ribosomal RNA
?CP000730 1998278 = NA (NA)intergenic (‑6/+5) USA300HOU_t0050/rrf4 tRNA‑Val/5S ribosomal RNA
* ? CP000730 748995 =82 (1.250)3 (0.050) 3/256 NT 3.6% coding (64/96 nt) USA300HOU_0695 hypothetical protein
?CP000730 749026 = 80 (1.240)coding (95/96 nt) USA300HOU_0695 hypothetical protein
* ? CP000730 = 80092868 (1.040)6 (0.100) 5/246 NT 7.5% intergenic (+62/‑289) hisC1/USA300HOU_0748 histidinol‑phosphate transaminase/hypothetical protein
?CP000730 801948 = 83 (1.340)intergenic (+189/+435) USA300HOU_0748/dgkA1 hypothetical protein/possible diacylglycerol kinase