Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A23 F20 I0 R1	95	76.5	1681216	93.1%	1565212	141.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	973,213	C→A	100%	E175* (GAG→TAG)	USA300HOU_0930 ←	possible HAD superfamily hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	973,213	0	C	A	100.0%	235.7 / ‑6.5	77	E175* (GAG→TAG)	USA300HOU_0930	possible HAD superfamily hydrolase
Reads supporting (aligned to +/- strand): ref base C (1/0); new base A (37/38); total (38/39)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ATCTTCATCACCGAATGCAATAATATTATTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTCAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGTTTGAATTCTTGGAT  >  CP000730/973076‑973344

aTCTTCATCACCGAATGCAATAATATTATTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAat                                                                                                                                  >  1:432276/1‑141 (MQ=255)

  cTTCATCACCGAATGCAATAATATTATTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAACGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAatat                                                                                                                                <  1:564448/141‑1 (MQ=255)

   ttCATCACCGAATGCAATAATATTATTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGGGTCGATGCGAAATATg                                                                                                                               >  2:5322/1‑141 (MQ=255)

    tcatcaCCGAATGCAATAATATTATTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCACCAGCGTCGATGTTAAATATGa                                                                                                                              >  2:207013/1‑141 (MQ=255)

     catcaCCGAATGCAATAATATTATTTCGGTTAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTCCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTCAATATGAt                                                                                                                             >  2:174083/1‑141 (MQ=255)

       tcaCCGAATGCAATAATATTATTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGTCCCAGCGTCGCTGCTAAATATGCTCg                                                                                                                           >  2:400762/1‑141 (MQ=255)

        caCCGAATGCTATAATATTATTTCGGGTAATATTTAAAAATTGTCTAACTTGCTAAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGAtaag                                                                                                                          <  2:237951/141‑4 (MQ=255)

        caCCGAATGCAATAATATTATTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATTACAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCgg                                                                                                                          <  1:295121/141‑1 (MQ=255)

           cgatggCAATAATATTATTTCGGTAAATATTTAAAAATTGTCTAATTTGCTCACTGCCTCTTGCTTTATTAATACCTCGTTGTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCAt                                                                                                                       <  2:362306/136‑1 (MQ=255)

             aaTGCAATAATATTATTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATaa                                                                                                                     >  1:407685/1‑141 (MQ=255)

             aaTGCAATAATATTATTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATaa                                                                                                                     >  1:803320/1‑141 (MQ=255)

             aaTGCAATAATATTATTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATaa                                                                                                                     >  1:665714/1‑141 (MQ=255)

              aTGCAATAATATTATTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATAGGCATaaa                                                                                                                    >  2:399732/1‑141 (MQ=255)

                gCAATAATATTATTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCCAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATaaaaa                                                                                                                  <  1:175841/141‑1 (MQ=255)

                gCAATAATATTATTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCCGCGTCGGTGCTAAATATGATCGGCATaaaaa                                                                                                                  >  1:70628/1‑141 (MQ=255)

                gCAATAATATTATTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATaaaaa                                                                                                                  <  1:705233/141‑1 (MQ=255)

                 cAATAATATTATTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGCATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAAt                                                                                                                 >  1:569982/1‑141 (MQ=255)

                 cAATAATATTATTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAAt                                                                                                                 >  1:340970/1‑141 (MQ=255)

                 cAATAATATTATTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAAt                                                                                                                 <  2:56939/141‑1 (MQ=255)

                 cAATAATATTATTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAAt                                                                                                                 >  2:481490/1‑141 (MQ=255)

                  aataatATTATTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATg                                                                                                                >  1:231905/1‑141 (MQ=255)

                    aaataTTATTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCGATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAg                                                                                                              <  1:504214/140‑1 (MQ=255)

                      atatTATTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACAAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTa                                                                                                            >  2:314898/1‑141 (MQ=255)

                        attattTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAg                                                                                                          <  1:757636/141‑1 (MQ=255)

                         ttattTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATAAAAAACAAAAAAATGAGTAAGc                                                                                                         <  2:675119/141‑1 (MQ=255)

                          tattTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCGTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCa                                                                                                        <  1:176005/141‑1 (MQ=255)

                                gatgaaaaataaaaaaaTGTCTAACTTGCTCAATGCCTCGTGCTTTATTAATACCACGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGTTGCTAAATAAAAAAAACAAAAAAATGAGTAAGCATAtttt                                                                                                  <  2:38985/130‑1 (MQ=255)

                                 gTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGACACAACGTCTATCCTAAATATGATCGGCATAAAAATAAGTAAGCATAttttt                                                                                                 >  2:712658/1‑141 (MQ=255)

                                    aaTATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGAt                                                                                              >  1:239343/1‑141 (MQ=255)

                                     atatTTAAAAATTGTCTAACTTGCTCAATGCCAGGATCTTTATTCATACTTTGATTTTCCCCATCACTGACAGCGAATGGTGCGCCCCAGCGTCGATGCGAAATTTGCTCGGCATAAAAATGAGTAAGCATATTTTTGAtt                                                                                             >  2:590847/1‑141 (MQ=255)

                                       atTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTc                                                                                           >  2:299944/1‑141 (MQ=255)

                                         ttAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATCAAACTGGGCACGCATGTTTTTGATTTCAg                                                                                         >  2:545477/1‑141 (MQ=255)

                                         taaaaaaTTGCTTAACTTGCTCAATGCCTCTTCCTTCATTAATACCAAGTTGTACAATTTCAATGACAGGGAATGGTGCGACCCAGCGTCGATGCTAAATATGAACGGCATAAAAAGGAGTAAGCATATTTTTGATTTCAg                                                                                         <  1:147285/139‑1 (MQ=255)

                                          tAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAgg                                                                                        >  1:726650/1‑141 (MQ=255)

                                             aaaTTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTAt                                                                                     >  2:687281/1‑141 (MQ=255)

                                              aaTTGTCTAACTTGCTCACTGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTAtt                                                                                    <  1:525605/141‑1 (MQ=255)

                                                  ttCTAACTCGCTCAATGCCTCCTCCTTTATTTATACCAAGTTTTACAATTTCCCTGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTAc                                                                                <  1:174083/140‑1 (MQ=255)

                                                  gTCTAAATTACGCAATGACTCTCGGTTTATTAATCGCCAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCTGCATAAAAATGAGTAAGCATATTTTTTATTTCAGGTATTTTAc                                                                                <  2:500543/141‑1 (MQ=255)

                                                    cTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACtt                                                                              >  2:18865/1‑141 (MQ=255)

                                                       aCTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTttct                                                                           <  1:597925/141‑1 (MQ=255)

                                                         ttGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTttcttc                                                                         <  2:718799/141‑1 (MQ=255)

                                                         ttGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTAAGGTATTTTACTttcttc                                                                         >  1:68310/1‑141 (MQ=255)

                                                          tGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCg                                                                        <  2:174715/141‑1 (MQ=255)

                                                            cTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGc                                                                      <  1:327326/141‑1 (MQ=255)

                                                               aaTGCCTCCTGCTTCATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGCCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTc                                                                   <  1:159384/141‑1 (MQ=255)

                                                                aTGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCa                                                                  <  1:111775/141‑1 (MQ=255)

                                                                  gCCTCGTGCTTTATTGCTGGCAAGGTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGGTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAAt                                                                <  2:627924/141‑1 (MQ=255)

                                                                      ctTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTaaa                                                            >  2:451662/1‑141 (MQ=255)

                                                                              attaATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGt                                                    <  1:729062/141‑1 (MQ=255)

                                                                                taATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAg                                                  <  2:102192/141‑1 (MQ=255)

                                                                                  aTACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAggg                                                >  1:327836/1‑141 (MQ=255)

                                                                                   tACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAgggg                                               <  2:669610/141‑1 (MQ=255)

                                                                                    aCCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCATGTATTTAACATTCTCCGGCTTCAATTAAAATTGAGGTAGGGGa                                              >  2:522966/1‑141 (MQ=255)

                                                                                     tcAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGAt                                             <  2:378093/140‑1 (MQ=255)

                                                                                         gTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCtt                                         <  2:451172/141‑1 (MQ=255)

                                                                                         gTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATACGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCtt                                         <  2:274548/141‑1 (MQ=255)

                                                                                           tttACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTc                                       >  2:353433/1‑141 (MQ=255)

                                                                                             tACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAGAATTGAGGTAGGGGGTTCTTTCag                                     >  2:654978/1‑140 (MQ=255)

                                                                                              aCAATTTCAATGACAGGGCATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAg                                    >  2:776829/1‑141 (MQ=255)

                                                                                                  tttCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGa                                <  1:545477/141‑1 (MQ=255)

                                                                                                    tCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACa                              <  1:649191/141‑1 (MQ=255)

                                                                                                    tCAATGACAGGGAATGGTGCCCCCCAGCCTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGAGTCTTTCAAGTGGACa                              <  1:623300/141‑1 (MQ=255)

                                                                                                      aaTGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGAGCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTGGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACagg                            >  2:59707/1‑139 (MQ=255)

                                                                                                       aTGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGt                           >  1:17500/1‑141 (MQ=255)

                                                                                                         gACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTACGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTaa                         >  1:514984/1‑141 (MQ=255)

                                                                                                         gACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACCAGtca                         >  1:103032/1‑139 (MQ=255)

                                                                                                         gACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTAAAGTGGACAAGTaa                         >  2:190959/1‑141 (MQ=255)

                                                                                                             gggAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTa                     <  1:314898/141‑1 (MQ=255)

                                                                                                              ggAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAGGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTATTTCAAGTGGACAAGTAAATTAc                    >  2:234069/1‑141 (MQ=255)

                                                                                                                  tGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTGCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGt                <  2:340970/141‑1 (MQ=255)

                                                                                                                   ggTGCGCCCCAGCGTCGATGCTTAATAAGATCGGAATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGtt               <  2:702448/141‑1 (MQ=255)

                                                                                                                   ggTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTGCTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACGAGtt               >  1:47164/1‑141 (MQ=255)

                                                                                                                    gTGCGCCCCTGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATAGTTTTGAGTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCCTTCAAGTGGACAAGTAAATTACCAGttt              <  2:14870/141‑1 (MQ=255)

                                                                                                                       cgcCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGTTTGaa           <  1:481490/141‑1 (MQ=255)

                                                                                                                             aGCGTCGATTCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGTGATTCTTTCAAGTGGACAAGTAAATTACCAGTTTGAATTCTTg     <  2:486817/141‑1 (MQ=255)

                                                                                                                             aGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGTTTGAATTATTg     >  1:265376/1‑141 (MQ=255)

                                                                                                                                gTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGTTTGAATTCTTGGAt  <  1:380007/141‑1 (MQ=255)

ATCTTCATCACCGAATGCAATAATATTATTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTCAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGTTTGAATTCTTGGAT  >  CP000730/973076‑973344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A