Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A19 F57 I0 R1
|
85 |
36.1 |
800162 |
93.0% |
744150 |
140.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
CP000730 |
1,196,508 |
C→A |
100% |
intergenic (+2563/‑1229) |
ftsQ → / → USA300HOU_1125 |
cell division protein FtsQ/hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | CP000730 | 1,196,508 | 0 | C | A | 100.0%
| 72.5
/ NA
| 25 | intergenic (+2563/‑1229) | ftsQ/USA300HOU_1125 | cell division protein FtsQ/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (13/12); total (13/12) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
TTCGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTCTAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAGAAATAGAGAAGAAA > CP000730/1196375‑1196642
|
ttCGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCCGAACAAGCGTAAATACTTATAGCAAt < 2:247398/141‑1 (MQ=255)
cacgATGAGATTGTTGTAACAGTTATGGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACt < 1:67676/138‑1 (MQ=255)
gttGAGATTGTTGGAACAGTTATTTCAACTTGTTTTGATGACAAACCAACAACACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCa < 2:91583/139‑1 (MQ=255)
aaCAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCAAATGCTTGATGGTGCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGc < 1:289945/141‑1 (MQ=255)
aGTTATTGTAGTTTGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTCATAGCTATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGcaca < 2:304129/141‑1 (MQ=255)
aGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATAATTCCCTTCAAATTCATCAAATGcaca > 2:97584/1‑141 (MQ=255)
aGTTATGGCAACTGGTTTGGATGACAAGCCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGcaca < 2:274616/141‑1 (MQ=255)
ttGCAACTGGTTTTGATAACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCaa < 1:324973/141‑1 (MQ=255)
tGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGAAACTTCTAAAGATGAATCATTCAATTCAAACTCATCAAATGCAAAAGCAAc > 2:259897/1‑141 (MQ=255)
tGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGAGTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAAc > 2:219557/1‑141 (MQ=255)
cgaaCATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCAAAAGCAACTGATAGTGTAAGTGAAAGAaca > 2:201683/3‑141 (MQ=255)
aCATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACAt > 2:162778/1‑141 (MQ=255)
cATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATa < 2:148080/141‑1 (MQ=255)
tCACATGGTCGTAAAACTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAACAACTGGTAGTGTAAGTAAAAGAACACATaaa > 2:107233/1‑139 (MQ=255)
aTGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGCCTCATTCTCGTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTa > 1:290239/1‑141 (MQ=255)
aTGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTACAGCCATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATGGTGTAAGTGAAAGAACGCATACagcca > 1:173683/1‑137 (MQ=255)
tGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAANTGATAGTGTAAGTGAAAGNACACATACAACTaa < 2:201333/141‑1 (MQ=255)
tGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGatat < 1:162778/141‑1 (MQ=255)
aCTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCCCAAGCAACTGATAGTGTAAg < 1:242450/101‑1 (MQ=255)
aCTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAg > 2:242450/1‑101 (MQ=255)
gATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGCTAGTGTAAGTGAAAGAACACATACAACTAAAGAAGAGGATATTCCTAGCTTCa > 1:351024/1‑141 (MQ=255)
gATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACCCATACAACTAAAGAAGATGATATTCCTAGCTTCa > 1:171194/1‑141 (MQ=255)
cGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAGAAATAgaga > 2:154282/1‑141 (MQ=255)
cGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAGAAATAgaga > 1:306228/1‑141 (MQ=255)
aTACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAGAAATAGAGaagaaa < 1:338818/141‑1 (MQ=255)
|
TTCGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTCTAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAGAAATAGAGAAGAAA > CP000730/1196375‑1196642
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
