Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A21 F63 I0 R1
|
92 |
32.2 |
718920 |
92.2% |
662844 |
140.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
CP000730 |
1,196,508 |
C→A |
100% |
intergenic (+2563/‑1229) |
ftsQ → / → USA300HOU_1125 |
cell division protein FtsQ/hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | CP000730 | 1,196,508 | 0 | C | A | 100.0%
| 64.0
/ NA
| 23 | intergenic (+2563/‑1229) | ftsQ/USA300HOU_1125 | cell division protein FtsQ/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (10/13); total (10/13) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
TTTCGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTCTAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAGAAATAGAGAAGAAAGAC > CP000730/1196374‑1196645
|
tttCGGTACAGTTATTAGTCCTTAATCACAAGAGTAGAGTGTTGTTAGTGTTATTTCCACTGGTTTTGTTGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCaa < 1:224331/141‑1 (MQ=255)
cGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAGATACTTATAGCAATGc > 1:359098/1‑141 (MQ=255)
ttattaATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTa < 1:133803/141‑1 (MQ=255)
atgaCAAGAATAGCTTGTAGTAACAGTGATTGTAACTGGTTTTTATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGACTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCAtt < 2:230887/138‑1 (MQ=255)
aTTACAATATGAGATTGTCGTAACAGTTATTGCAACAGGTTTTGATCACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCAtt < 2:144905/141‑1 (MQ=255)
aTTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATACAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCa < 1:25291/141‑1 (MQ=255)
gCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAACCAACt > 2:281807/1‑141 (MQ=255)
aaCTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGa > 1:307184/1‑141 (MQ=255)
aCTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGAt > 1:201593/1‑141 (MQ=255)
gTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGTTTTCTAGCAACCGTATATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAgtg < 1:244304/141‑1 (MQ=255)
tgatgaCAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAg < 1:35313/141‑1 (MQ=255)
atgaCAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTg < 1:325670/141‑1 (MQ=255)
tCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACa < 2:177639/141‑1 (MQ=255)
tCACAAGGTCGTAAAACTGGTAGCACTGGATTCGGAACAAACGTAAATAGTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTAATCAAATGCCCAAGCAACCGATAGTGAAAGTAAAAGAAAACATACa > 2:17336/1‑141 (MQ=255)
caTGGTCGTAAATCTGGTAGGAGAAGGTCCTTAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACt > 1:85437/1‑141 (MQ=255)
cTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCt < 1:141812/141‑1 (MQ=255)
ggATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTc > 2:316670/1‑141 (MQ=255)
tCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTa > 2:263132/1‑141 (MQ=255)
cGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAGGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAg > 1:182173/1‑141 (MQ=255)
cGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCCAGCTTCATTAg > 1:59900/1‑141 (MQ=255)
cTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAACTTCATCAAATGCACAACCAACTGATAGTGTAAGTGAAAGAACACAGACAACTAAATCAGATGATATTCCTAGCTTAATTAGAAATAGAGAAGAAAGAc < 1:174866/141‑1 (MQ=255)
cTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGCTAAACCAATCGTCATTAGAAATAGAGAAGAAAGAc < 1:85224/141‑1 (MQ=255)
cTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAGAAATAGAGAAGAAAGAc < 1:306714/141‑1 (MQ=255)
|
TTTCGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTCTAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAGAAATAGAGAAGAAAGAC > CP000730/1196374‑1196645
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
