Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A15 F177 I0 R1
|
51 |
26.9 |
575130 |
96.6% |
555575 |
140.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
CP000730 |
1,238,307 |
C→T |
100% |
Q141* (CAA→TAA) |
USA300HOU_1162 → |
glycerone kinase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | CP000730 | 1,238,307 | 0 | C | T | 100.0%
| 52.6
/ NA
| 18 | Q141* (CAA→TAA) | USA300HOU_1162 | glycerone kinase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (11/7); total (11/7) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
GTAAAAATATTGAAAGTGAATCTGAAATTAATTCAAAATTGTTAGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGCAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAACACACCAAACTTATTAGCTGTACTTAAAGAAGTTGGTGTTGTT > CP000730/1238170‑1238438
|
gTAAAAATATTGAAAGTGAATCTGAAATTAATTCAAAATTGTTAGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAg > 2:46723/1‑141 (MQ=255)
ttGAAAGTGAATCTGAAATTAATTCAAAATTGTTAGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAg > 1:117193/1‑141 (MQ=255)
tGAAAGTGAATCTGATATTAATTCAAGATTGTTATCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGCAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGa < 1:173823/141‑1 (MQ=255)
ttaattCAAAATTGTTAGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATAcag > 2:146101/1‑139 (MQ=255)
ttaattCAAAATTGTTAGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAACCTGCAATAGAAAAAGCAAATAATACTg > 2:144982/1‑141 (MQ=255)
aattCAAAATTGTTAGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACGGaa < 1:26391/141‑1 (MQ=255)
aTTGTTAGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCCATAGAAAAAGCAAATAATACTGAAGATTGtat < 1:222533/141‑1 (MQ=255)
ttGTTAGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGtata < 2:284887/141‑1 (MQ=255)
tCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACat > 1:188056/1‑141 (MQ=255)
tgtTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTaaa > 2:248461/1‑141 (MQ=255)
gtTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTaaaa < 1:132080/141‑1 (MQ=255)
ggCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGa > 1:282135/1‑141 (MQ=255)
cATATAAAGCTGTTATGAAACCAGTCGACAGTACAATACTTACAATGGCAAAAGATGCTACGTAAGCTGCAATAGAAACGACAACAACGCCGGAACCACGTCTAGAATGAATGGAGGAAATTATTGTaaagagcgatgcac > 2:104441/1‑130 (MQ=255)
ttGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAACACACCAAACttat > 2:169033/1‑141 (MQ=255)
tACTTACAGTTGCAAAAGATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAACACACCAAACTTATTAGCTGTACTTa < 1:46723/141‑1 (MQ=255)
aaGATGCTGCGTAAGCTGCAATAGAAAAAGCAAGTAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAACACACCAAACTTATTAGCTGTACTTAAAGAAGTTGGtgttt > 1:13576/1‑140 (MQ=255)
gATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAACACACCAAACTTATTAGCTGTACTTAAAGAAGTTGGTgttgtt < 1:548/141‑1 (MQ=255)
gATGCTGCGTAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAACACACCAAACTTATTAGCTGTACTTAAAGAAGTTGGTgttgtt > 2:548/1‑141 (MQ=255)
|
GTAAAAATATTGAAAGTGAATCTGAAATTAATTCAAAATTGTTAGCTGAAAGTTTTCAAGCTGGTGTTGAAACGGCATATAAAGCTGTTATGAAACCAGTTGAAGGTACAATACTTACAGTTGCAAAAGATGCTGCGCAAGCTGCAATAGAAAAAGCAAATAATACTGAAGATTGTATAGAATTAATGGAGTACATTATTGTAAAAGCCAATGAATCACTTGAAAACACACCAAACTTATTAGCTGTACTTAAAGAAGTTGGTGTTGTT > CP000730/1238170‑1238438
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
