Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A15 F177 I0 R1	51	26.9	575130	96.6%	555575	140.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,883,654	C→T	100%	G522D (GGT→GAT)	fmtB1 ←	cell surface anchored protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,883,654	0	C	T	100.0%	84.6 / ‑6.5	29	G522D (GGT→GAT)	fmtB1	cell surface anchored protein
Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (19/9); total (19/10)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AATCTTGTGTATATGTTTTATACGTTAATGTCTCGTTAAATGTTACTGTTCTTGGTGTCGGCACATTATTTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTACCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGCATCATATGTAACATTCATATCATTAACATCAACGCCTGAATTGTTACTTGGAAAATCTTTAGTCAATGAATTGTTCACATATTCAACACCTTCAG  >  CP000730/1883517‑1883789

aaTCTTGTGTATATGTTTTATACGTTAATGTCTCGTTAAATGTTACTGTTCTTGGTGTCGGCACATTATTTACACGTAATTTATATCTTAAATCGAGGAGTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTAtctc                                                                                                                                      <  1:269841/141‑1 (MQ=255)

 aTCTTGTGTATATGTTTTATACGTTAATGTCTCGTTAAATGTTACTGTTCTTGGTGTCGGTACATTATTTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTAtctcc                                                                                                                                     <  2:205692/141‑1 (MQ=255)

      gtgtATATGTTTTATACGTTAATGTCTCGTTAAATGTTACTGTTCTTGGTGTCGGCACATTATTTACACGTAATTTATCTCTTAAAACGAGTATTTTATCAGGCATAAGTCGTGCCGGAGCGTTTGCTGTATCTCATCCat                                                                                                                                >  1:65949/1‑140 (MQ=255)

      gtgtATATGTTTTATACGTTAATGTCTCGTTAAATGTTACTGTTCTTGGTGTCGGCACATTATTTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCCCCAg                                                                                                                                >  2:135069/1‑141 (MQ=255)

          ttatGTTTTATATGTTGATTTCTCGTTAAATTGTACTGTTCTTGGTGTCGGCACATTATTTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACt                                                                                                                            <  2:40986/140‑1 (MQ=255)

                    taCGTTAATGTCTCGTTAAATGTTACTGTTCTTGGTGTCGGCACATTATTTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGtt                                                                                                                  >  1:263096/1‑141 (MQ=255)

                     aCGTTAATGTCTCGTTAAATGTTACTGTTCTTGGTGTCGGCACATTATTTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTa                                                                                                                 >  2:121540/1‑141 (MQ=255)

                             gTCTCGTTAAATGTTACTGTTCTTGGTGTCGGCACATTATTTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGa                                                                                                         >  2:18636/1‑141 (MQ=255)

                               ctcGTTAAATGTTACTGTTCTTGGTGTCGGCACATTATTTACACGTAATTTATATTTATAATCCATTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGAtt                                                                                                       <  1:136032/141‑1 (MQ=255)

                               ctcGTTAAATGTTACTGTTCTTGGTGTCGGCACATTATTTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGAtt                                                                                                       >  1:189405/1‑141 (MQ=255)

                                ttgTTAAATGTTTCTGTTCTTGGTGTCTGCACATTATTTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCATTACTTTTAATTGTTATCACTCGAttt                                                                                                      <  2:65390/139‑1 (MQ=255)

                                tcGTTAAATTCTACTGTCTTTGTTGTCGGCAAATGATTTACGCGTAATTTAGATATTAATTCTATTATTTTATCAGGCATAAGTCGTGCCGGAGATTTTGTTGTATCTCCTCCAGTACTTTTAATTGTTATCACTCGAttt                                                                                                      <  2:250658/141‑1 (MQ=255)

                                  gTTAAATGTTACTGTTCTTGGTGTCGGCACATTATTTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTtgc                                                                                                    <  1:278073/141‑1 (MQ=255)

                                     aaaTGTTACTGTTCTTGGTGTCGGCACATTATTTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTtgctgc                                                                                                 <  1:182197/141‑1 (MQ=255)

                                         gTTACTGTTCTTGGTGTCGGCACATTATTTAAACGTAATTTCTATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCTCACTATTTTCTGaatca                                                                                             >  2:228449/1‑141 (MQ=255)

                                              tGTTCTTGGTGTCGGCACATTATTTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGGTTTGCTGCATCctattt                                                                                        >  1:160874/1‑139 (MQ=255)

                                                       tgtCGGCACATTATTTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGCATCATATGTAACATTCat                                                                               >  2:165421/1‑141 (MQ=255)

                                                             cacaTTATTTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTACTGCCTCCATTACTTTTAATTGTTATCACACGATTTGCTGCATCATATGTAACATTCATATCAtt                                                                         <  2:245279/141‑1 (MQ=255)

                                                                  tattTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGCATCATATGTAACATTCATATCATTAACAt                                                                    >  1:6350/1‑141 (MQ=255)

                                                                         acGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGCATCATATGTAACATTCATATCATTAACATCAACGcc                                                             >  1:189450/1‑141 (MQ=255)

                                                                         acGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGCATCATATGTAACATTCATATCATTAACATCAACGcc                                                             >  1:80598/1‑141 (MQ=255)

                                                                                  ttatCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGCATCATATGTAACATTCATATCATTAACATCAACGCCTGAATTGtt                                                    <  2:286400/140‑1 (MQ=255)

                                                                                  atatCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGCATCATATGTAACATTCATATCATTAACATCAACGCCTGAATTGtt                                                    >  2:158897/1‑141 (MQ=255)

                                                                                     tcgtAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGCATCATATGTAACATTCATATCATTAACATCAACGCCTGAATTGTTACt                                                 <  2:13533/138‑1 (MQ=255)

                                                                                               aGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGCATCATATGTAACATTCATATCATTAACATCAACGCCTGAATTGTTACTTGGAAAATCt                                       >  2:41987/1‑141 (MQ=255)

                                                                                                gtcttttATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGCATCATATGTAACATTCATATCATTAACATCAACGCCTGAATTGTTACTTGGAAAATCtt                                      <  1:121540/138‑1 (MQ=255)

                                                                                                gTATTTTATCAGGCATACCCCGTTCCTGTGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGCATCATATGTAACATTCATATCATTCACATCAACGCCTGAATTGTTACTTGGAAAATCtt                                      >  2:203528/1‑141 (MQ=255)

                                                                                                   ttttATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGCATCATATGTAACATTCATATCATTAACATCAACGCCTGAATTGTTACTTGGAAAATCTTTAg                                   >  2:260958/1‑141 (MQ=255)

                                                                                                            gCATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGCATCATATGTAACATTCATATCATTAACATCAACGCCTGAATTGTTACTTGGAAAATCTTTAGTCAATGAAt                          >  2:112568/1‑141 (MQ=255)

                                                                                                             cATAAGTCGTGCCGGAGAGTTTGCTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGCATCATATGTAACATTCATATCATTAACATCAACGCCTGAATTGTTACTTGGAAAATCTTTAGTCAATGAAtt                         >  2:23066/1‑141 (MQ=255)

                                                                                                                                    cTGTATCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGAAGCATCATATGTAACATTCATATCATTAACATCAACGCCTGAATTGTTACTTGGAAAATCTTTAGTCAATGAATTGTTCACATATTCAACACCTTCAg  >  1:270035/1‑141 (MQ=255)

AATCTTGTGTATATGTTTTATACGTTAATGTCTCGTTAAATGTTACTGTTCTTGGTGTCGGCACATTATTTACACGTAATTTATATCTTAAATCGAGTATTTTATCAGGCATAAGTCGTGCCGGAGAGTTTGCTGTACCTCCTCCAGTACTTTTAATTGTTATCACACGATTTGCTGCATCATATGTAACATTCATATCATTAACATCAACGCCTGAATTGTTACTTGGAAAATCTTTAGTCAATGAATTGTTCACATATTCAACACCTTCAG  >  CP000730/1883517‑1883789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A