Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A17 F109 I1 R1 28 33.5 644026 99.3% 639517 151.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP000730 2,266,814 G→T R251R (CGA→AGA)  USA300HOU_2140 ← SAP domain protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,266,8140GT100.0% 59.2 / NA 17R251R (CGA→AGA) USA300HOU_2140SAP domain protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (9/8);  total (9/8)

CTACTTTCTTTTTTTACATTAGACACTACATAGTTTATGAACTTTTCAATCAATTGTTCTTCCAAATCACTATAATCTTGTGTGTCCTTTTTAAATAATTCAAACATAATATTATTGGATAAATTTTCATTATATATTAATCGCTCGTACATACCAGAAGCGCTAGAGTTGATAAATCTATTCAGATCGTTTTGTATTTCGTTCAAATCTATATTCCCTTTACTATCGTAATATGAATAATTACTCATTAATTTTTTTAAGAAATCTCTCAAACCAAAGTA  >  CP000730/2266672‑2266952
                                                                                                                                              |                                                                                                                                          
cTACTTTCTTTTTTTACATTAGACACTACATAGTTTATGAACTTTTCAATCAATTGTTCTTCCAAATCACTATAATCTTGTGTGTCCTTTTTAAATAATTCAAACATAATATTATTGGATAAATTTTCATTATATATTAATCTCTCGTACa                                                                                                                                    <  1:289600/151‑1 (MQ=255)
                     gACACTACATAGTTTATGAACTTTTCAATCAATTGTTCTTCCAAATCACTATAATCTTGTGTGTCCTTTTTAAATAATTCAAACATAATATTATTGGATAAATTTTCATTATATATTAATCTCTCGTACATACCAGAAGCGCTAGAGTTGa                                                                                                               >  2:62115/1‑151 (MQ=255)
                      acacTACATAGTTTATGAACTTTTCAATCAATTGTTCTTCCAAATCACTATAATCTTGTGTGTCCTTTTTAAATAATTCAAACATAATATTATTGGATAAATTTTCATTATATATTAATCTCTCGTACATACCAGAAGCGCTAGAGTTGAt                                                                                                              <  2:237374/151‑1 (MQ=255)
                                gTTTATGAACTTTTCAATCAATTGTTCTTCCAAATCACTATAATCTTGTGTGTCCTTTTTAAATAATTCAAACATAATATTATTGGATAAATTTTCATTATATATTAATCTCTCGTACATACCAGAAGCGCTAGAGTTGATAAATCTATTc                                                                                                    <  1:42389/151‑1 (MQ=255)
                                  ttATGAACTTTTCAATCAATTGTTCTTCCAAATCACTATAATCTTGTGTGTCCTTTTTAAATAATTCAAACATAATATTATTGGATAAATTTTCATTATATATTAATCTCTCGTACATACCAGAAGCGCTAGAGTTGATAAATCTATTCAg                                                                                                  >  2:48781/1‑151 (MQ=255)
                                      gAACTTTTCAATCAATTGTTCTTCCAAATCACTATAATCTTGTGTGTCCTTTTTAAATAATTCAAACATAATATTATTGGATAAATTTTCATTATATATTAATCTCTCGTACATACCAGAAGCGCTAGAGTTGATAAATCTATTCAGATCg                                                                                              >  1:10036/1‑151 (MQ=255)
                                      gAACTTTTCAATCAATTGTTCTTCCAAATCACTATAATCTTGTGTGTCCTTTTTAAATAATTCAAACATAATATTATTGGATAAATTTTCATTATATATTAATCTCTCGTACATACCAGAAGCGCTAGAGTTGATAAATCTATTCAGATCg                                                                                              >  1:27772/1‑151 (MQ=255)
                                      gAACTTTTCAATCAATTGTTCTTCCAAATCACTATAATCTTGTGTGTCCTTTTTAAATAATTCAAACATAATATTATTGGATAAATTTTCATTATATATTAATCTCTCGTACATACCAGAAGCGCTAGAGTTGATAAATCTATTCAGATCg                                                                                              >  1:303172/1‑151 (MQ=255)
                                                             ccAAATCACTATAATCTTGTGTGTCCTTTTTAAATAATTCAAACATAATATTATTGGATAAATTTTCATTATATATTAATCTCTCGTACATACCAGAAGCGCTAGAGTTGATAAATCTATTCAGATCGTTTTGTATTTCGTTCAAATCtat                                                                       <  1:142905/151‑1 (MQ=255)
                                                             acAAATCACTATAATCTTGTGTGTCCTTTTTAAATAATTCAAACATAATATTATTGGATAAATTTTCATTATATATTAATCTCTCGTACATACCAGAAGCGCTAGCGTTGATAAATCTATTCAGATCGTTTTGTATTTCGTTCAAATCtat                                                                       <  2:207354/150‑1 (MQ=255)
                                                                       ataATCTTGTGTGTCCTTTTTAAATAATGCAAACATAATATTATTGGATAAATTTTCATTATATATTAATCTCTCGTACATACCAGAAGCGCTAGAGTTGATAAATCTATTCAGATCGTTTTGTATTTCGTCCAAATCCATATTCCCTTTa                                                             >  2:263016/1‑151 (MQ=255)
                                                                                           tAAATAATTCAAACATAATATTATTGGATAAATTTTCATTATATATTAATCTCTCGTACATACCAGAAGCGCTAGAGGTGATAAATCGATTCAGATCGTTTTGTATTTCGTTCAAATCTATATTCCCTTTACTATCGTAATATGAATAAtt                                         >  1:112052/1‑151 (MQ=255)
                                                                                             aataatTCAAACATAATATTATTGGATAAATTTTCATTATATATTAATCTCTCGTACATACCAGAAGCGCTAGAGTTGATAAATCTATTCAGATCGTTTTGTATTTCGTTCAAATCTATATTCCCTTTACTATCGTAATATGAATAATTAc                                       >  1:124791/1‑151 (MQ=255)
                                                                                                        cATAATATTATTGGATAAATTTTCATTATATATTAATCTCTCGTACATACCAGAAGCGCTAGAGTTGATAAATCTATTCAGATCGTTTTGTATTTCGTTCAAATCTATATTCCCTTTACTATCGTAATATGAATAATTACTCATTAAtttt                            >  1:18002/1‑151 (MQ=255)
                                                                                                         ataataTTATTGGATAAATTTTCATTATATATTAATCTCTCGTACATACCAGAAGCGCTAGAGTTGATAAATCTATTCAGATCGTTTTGTATTTCGTTCAAATCTATATTCCCTTTACTATCGTAATATGAATAATTACTCATTAAttttt                           <  2:95370/151‑1 (MQ=255)
                                                                                                                                  tatataTTAATCTCTCGTACATACCAGAAGCGCTAGAGTTGATAAATCTATTCAGATCGTTTTGTATTTCGTTCAAATCTATATTCCCTTTACTATCGTAATATGAATAATTACTCATTAATTTTTTTAAGAAATCTCTCAAACCAAAGTa  <  1:117703/151‑1 (MQ=255)
                                                                                                                                  tatataTTAATCTCTCGTACATACCAGAAGCGCTAGAGTTGATAAATCTATTCAGATCGTTTTGTATTTCGTTCAAATCTATATTCCCTTTACTATCGTAATATGAATAATTACTCATTAATTTTTTTAAGAAATCTCTCAAACCAAAGTa  <  1:1910/151‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                          
CTACTTTCTTTTTTTACATTAGACACTACATAGTTTATGAACTTTTCAATCAATTGTTCTTCCAAATCACTATAATCTTGTGTGTCCTTTTTAAATAATTCAAACATAATATTATTGGATAAATTTTCATTATATATTAATCGCTCGTACATACCAGAAGCGCTAGAGTTGATAAATCTATTCAGATCGTTTTGTATTTCGTTCAAATCTATATTCCCTTTACTATCGTAATATGAATAATTACTCATTAATTTTTTTAAGAAATCTCTCAAACCAAAGTA  >  CP000730/2266672‑2266952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A