Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A17 F172 I0 R1	42	33.2	716278	96.8%	693357	139.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	2,372,863	C→G	36.7%	G305R (GGA→CGA)	pbuG ←	NCS2 family nucleobase:cation symporter‑2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,372,863	0	C	G	36.7%	23.7 / 45.3	41	G305R (GGA→CGA)	pbuG	NCS2 family nucleobase:cation symporter‑2
Reads supporting (aligned to +/- strand): ref base C (14/12); new base G (10/5); total (24/17)
Fisher's exact test for biased strand distribution p-value = 5.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.46e-01

GATTAACGGACTAAAGAACAGCGCTAATAGGAAACAACAACCTGTTACGATACTAGCAAATCCTGTTCTAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAAC  >  CP000730/2372726‑2372996

gATTAACGGACTAAAGAACAGCGCTAATAGGAAACAACAACCTGTTACGATACTAGCAAATCCTGTTCTAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCCTa                                                                                                                                     <  2:337713/140‑1 (MQ=255)

   tAACGGACTAAAGAACAGCGCTAATAGGAAACAACAACCTGTCACGATACTAGCAAATCCGGTTCTAGCACCTACAGCAACACCTGACGTTGATTCGATATAAGAAGTTGTTGTAGTAGTTCCAAAAACTGATACTCCac                                                                                                                                  >  2:26013/1‑139 (MQ=255)

    aaCGGACTAAAGAACAGCGCTAATAGGAAACAACAACCTGTTACGATACTAGCAAATCCTGTTCTAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCCTACAAt                                                                                                                                 >  2:107934/1‑140 (MQ=255)

     aCGGACTAAAGAACAGCGCTAATAGGAAACAACAACCTGTTACGATACTAGCAAATCCTGTTCTAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATc                                                                                                                                <  1:179625/140‑1 (MQ=255)

          cTAAAGAACAGCGCTAATAGGAAACAACAACCTGTTACGATACTAGCAAATCCTGTTCTAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTTATCCTACAATCGTAGc                                                                                                                           >  2:40999/1‑140 (MQ=255)

             aaGAACACCGCTAATAAGAAACAACAACCTGTTACGATACTAGCAAATCCTGTTCTAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTaa                                                                                                                        <  1:227015/140‑1 (MQ=255)

                 aCAGGGCTAAGAGGAAACAAGAACCTGTTACGATACTAGCAAATCCTGTTCTCGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTAAAGaa                                                                                                                    <  2:104880/140‑1 (MQ=255)

                    gcgcTAATAGGAAACAACAACCTGTTACGATACTAGCAAATCCTGTTCTAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTAAAGAATCt                                                                                                                 >  2:332020/1‑140 (MQ=255)

                     cgcAAATAGCAAACAACAACCTGTTACGATACTAGCAAATCCTGTTCTACCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTAAAGAATCTg                                                                                                                <  2:96606/140‑1 (MQ=255)

                         aaTAGGAAACAACAACATGTTACGATACTAGCAAATCCTGTTCTAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGaa                                                                                                            >  2:77412/1‑140 (MQ=255)

                                 acaacaacCTGTTACGATACTAGCAAATCCTGTTCTAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGctc                                                                                                    >  1:296170/1‑140 (MQ=255)

                                    tcaacCTGTTACGATACTAGCAAATCCTGTTCTAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTGGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTAc                                                                                                 <  2:152878/139‑1 (MQ=255)

                                      aacCTGTTACGATACTAGCAAATCCTGTTCTAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCCTACAATCGTCGCTAAAGAATCTGAGAACAATGCTCTACCa                                                                                               >  1:331450/1‑140 (MQ=255)

                                      aacCTGTTACGATACTAGCAAATCCTGTTCTAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCa                                                                                               >  2:74670/1‑140 (MQ=255)

                                       acCTGTTACGATACTAGCAGATCCTGTTCTAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAg                                                                                              >  1:213402/1‑140 (MQ=255)

                                        ccTGTTACGATACTAGCAAATCCTGTTCTAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGc                                                                                             >  2:114188/1‑140 (MQ=255)

                                                              cTGTTCTAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTc                                                                       >  1:108608/1‑140 (MQ=255)

                                                               tGTTCTAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTACTATCTTTCa                                                                      <  1:261938/140‑1 (MQ=255)

                                                                gTTCTAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCat                                                                     >  1:216620/1‑140 (MQ=255)

                                                                gTTCTAGCACCTACAGCAACACCTGAAGTTGATGCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCat                                                                     >  1:71683/1‑140 (MQ=255)

                                                                    tttccccTACAGCAACTCCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAATAACTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCataata                                                                 <  1:80296/135‑1 (MQ=255)

                                                                       cACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGGTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTCCCAGCTCGTGCTAATGTGTTCTCTTTCATAATACCt                                                              >  1:342411/1‑140 (MQ=255)

                                                                        aCCTACAGCAACACCTGAAGTTGAGTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCGCTTGGTAATTTATTATCTTTCATAATACCTg                                                             <  2:164834/140‑1 (MQ=255)

                                                                            aCAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTg                                                         >  1:150285/1‑140 (MQ=255)

                                                                            aCAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAATTAATCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTg                                                         >  1:187771/1‑140 (MQ=255)

                                                                               gCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGt                                                      >  1:141630/1‑140 (MQ=255)

                                                                                     ccTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTCGGTAATTTATTATCTTTAATAATACCTGCttacgtacccac                                                >  2:22705/1‑130 (MQ=255)

                                                                                          aGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACAGcta                                           <  1:107934/140‑1 (MQ=255)

                                                                                                 tCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACAGCTCCTCATGt                                    >  2:88020/1‑140 (MQ=255)

                                                                                                     tataAGAAGTTGTTGTAGTTGTTCCAAAAACTGATTGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAATAGCTACTAATGTACCa                                <  1:197628/140‑1 (MQ=255)

                                                                                                     tataAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACAGCTACTAATGTACCa                                >  2:90025/1‑140 (MQ=255)

                                                                                                       taAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACAGCTACTAATGTACCAGc                              <  1:77412/140‑1 (MQ=255)

                                                                                                             gttgttGTAGTTGTTCCAAAATCTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACAGCTACTAATGTACCAGCTGTATc                        <  1:327290/140‑1 (MQ=255)

                                                                                                             gttgttGTAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACAGCTACTAATGTACCAGCTGTATc                        >  2:9673/1‑140 (MQ=255)

                                                                                                             ctttttgtAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCGCTACCAGCTCTTGGGAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACAGCTACTAATGTACCAGCTGTATc                        <  2:149092/136‑1 (MQ=255)

                                                                                                                   gtAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCGCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCACCAGGTACTAATCTACCAGCGGTATCAAAGaa                  >  2:80271/1‑140 (MQ=255)

                                                                                                                             cAAAAACTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCaataaat        <  1:181244/140‑1 (MQ=255)

                                                                                                                               aaaaCTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAataaataa      <  1:74670/140‑1 (MQ=255)

                                                                                                                                 aaCTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGa    <  2:296170/140‑1 (MQ=255)

                                                                                                                                   cTGATCGTACAATCGCAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACAGCTACTAATGTACCAGCTGTGTTAAAGAGATCAATAAATAAGAAc  >  1:264134/1‑140 (MQ=255)

                                                                                                                                   cTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTCCCAGCTCTAGGTAATTAATTATCTTTCATAATACCAGCTTGAGTACCATCAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATACGaaa  >  2:87503/1‑139 (MQ=255)

GATTAACGGACTAAAGAACAGCGCTAATAGGAAACAACAACCTGTTACGATACTAGCAAATCCTGTTCTAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAAC  >  CP000730/2372726‑2372996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A