Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F67 I1 R1	27	23.6	499472	97.1%	484987	140.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	225,004	G→T	E31* (GAA→TAA)	murQ →	possible sugar phosphate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	225,004	0	G	T	100.0%	78.5 / NA	28	E31* (GAA→TAA)	murQ	possible sugar phosphate isomerase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (16/12); total (16/12)

GCCGAATGATACATTTGATTTTCAAAAAACTAGGGAGTGTAAGAAGTGATGGAAAATAGTACGACCGAAGCGCGTAATGAAGCGACGATGCATCTTGATGAAATGACTGTGGAAGAGGCTTTAATTACGATGAATAAAGAAGATCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGCAGGTACAAGTGGAAGGTTGGGTGTC  >  CP000730/224866‑225141

gCCGAATGATACATTTGATTTTCAAAAAACTAGGGAGTGTAAGAAGTGATGGAAAATAGTACGACCGAAGCGCGTAATGAAGCGACGATGCATCTTGATGAAATGACTGTGGAAGAGGCTTTAATTACGATGAataaataa                                                                                                                                         >  1:354461/1‑141 (MQ=255)

     aTGATACATTTGATTTTCAAAAAACTAGGGAGTGTAAGAAGTGATGGAAAATAGTACGACCGAAGCGCGTAATGAAGCGACGATGCATCTTGATGAAATGACTGTGGAAGAGGCTTTAATTACGATGAATAAATAAGATCa                                                                                                                                    >  1:42586/1‑141 (MQ=255)

       gATACATTTGATTTTCAAAAAACTAGGGAGTGTAAGAAGTGATGGAAAATAGTACGACCGAAGCGCATAATTAAGCTACGATGCAACTTGATGAAATGACTGTGGAAGAGGCTTTAATTACGATGAATAAATAAGATCAGc                                                                                                                                  <  1:363555/141‑1 (MQ=255)

        aTACATTTGATTTTCAAAAAACTAGGGAGTGTAAGAAGTGATGGAAAATAGTACGACCGAAGCGCGTAATGAAGCGACGATGCATCTTGATGAAATGACTGTGGAAGAGGCTTTAATTACGATGAATAAATAAGATCAGCa                                                                                                                                 <  1:448641/141‑1 (MQ=255)

        aTACATTTGATTTTCAAAAAACTAGGGAGTGTAAGAAGTGATGGAAAATAGTACGACCGAAGCGCGTAATGAAGCGACGATGCATCTTGATGAAATGACTGTGGAAGAGGCTTTAATTACGATGAATAAATAAGATCAGCa                                                                                                                                 <  1:398800/141‑1 (MQ=255)

         tACATTTGATTTTCAAAAAACTAGGGAGTGTAAGAAGTGATGGAAAATAGTACGACCGAAGCGCGTAATGAAGCGACGATGCATCTTGATGAAATGACTGTGGAAGAGGCTTTAATTACGATGAATAAATAAGATCAGCaa                                                                                                                                <  1:467190/141‑1 (MQ=255)

                 aTTTTCAAAAAACTAGGGAGTGTAAGAAGTGATGGAAAATAGTACGACCGAAGCGCGTAATGAAGCGACGATGCATCTTGATGAAATGACTGTGGAAGAGGCTTTAATTACGATGAAAAAATAAGACCGGCAAGGCCCGtt                                                                                                                        >  1:172683/1‑141 (MQ=255)

                    ttCAAAAACCTTGGGAGTGTAAGAAGTGATGGAAAATAGTACGACCGAAGCGCGTAATGAAGCGACGATGCATCTTGATGAAATGACTGTGGAAGAGGCTTTAATTACGATGAATAAATAAGATCAGCAAGTCCCGTTAGc                                                                                                                     <  1:198957/141‑1 (MQ=255)

                         aaaaCTAGGGAGTGTAAGAAGTGATGGAAAATAGTACGACCGAAGCGCGTAATGAAGCGACGATGCATCTTGATGAAATGACTGTGGAAGCGGCATTAATTACGATGAATAAATAAGATCAGCAAGTCCCGTTAGCAGTTc                                                                                                                <  1:95750/141‑1 (MQ=255)

                           aaCTAGGGAGTGTAAGAAGTGATGGAAAATAGTACGACCGAAGCGCGTAATGAAGCGACGATGCATCTTGATGAAATGACTGTGGAAGAGGCTTTAATTACGATGAATAAATAAGATCAGCAAGTCCCGTTAGCAGTTCGa                                                                                                              <  1:170792/141‑1 (MQ=255)

                            aCTAGGGAGAGTAAGAAGTGATGGAAAATAGTACGACCGAAGCGCGTGATGAAGCGACGATGCATCTTGATGAGATGACTGTGGAAGAGGCTTTAATTACGATGAATAAATAAAATCAGCAAGTCCCGTTAGCAGTTCGaa                                                                                                             >  1:171456/1‑141 (MQ=255)

                                      gtAAGAAGTGATGGAAAATAGTACGACCGAAGCGCGTAATGAAGCGACGATGCATCTTGATGAAATGACTGTGGAAGAGGCTTTAATTACGATGAATAAATAAGATCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATAcc                                                                                                   >  1:433878/1‑141 (MQ=255)

                                           aagTGATGGAAAATAGTCAGAGACTAACGTTTAATGAAGCGACGATGCATCTTGATGAAATGACTGTGGACGAGGCTTTAATTACAATGAATAAATAAGATCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAAt                                                                                              >  1:118683/1‑141 (MQ=255)

                                                 tGGAAAATAGTACGACCGAAGCGCGTAATGAGGCGACGATGCATCTTGATGAAATGACTGTGGAAGAGGCTTTAATTACGATGAATAAATAAGATCAGCAAGTCCCGTTAGCAGTTAGAAAGGCAATACCACAATTGACaa                                                                                        >  1:98640/1‑141 (MQ=255)

                                                  ggAAAATAGTACGACCGAAGCGCGTAATGAAGCGACGATGCATCTTGATGAAATGACTGTGGAAGAGGCTTTAATTACGATGAATAAATAAGATCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTCACaaa                                                                                       >  1:414313/1‑141 (MQ=255)

                                                                     gcgcGTAATGAAGCGACGATGCATCTTGATGAAATGACTGTGGAAGAGGCTTTAATTACGATGAATAAATAAGATCAGCAAGTCCCGTTAGCAGTTAGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAAtt                                                                    >  1:467847/1‑141 (MQ=255)

                                                                         gTAATGAAGCGACGATGCAACTTGATGAAATGACTGTGGAAGAGGCTTTAATTACGATGAATAAATAAGATCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGcac                                                                >  1:297125/1‑141 (MQ=255)

                                                                            aTGAAGCGACGATGCATCTTGATGAAATGACTGTGGAAGAGGCTTTAATTACGATGAATAAATAAGATCAGCAAGTCCCGTTAGCAGTTCGACAGGCAATTCCTTAATTGACAAAAGTAATTAAAAAAACAATTGCACAGt                                                             <  1:217836/141‑1 (MQ=255)

                                                                                   gacgaTGCATCTTGATGAAATGACTGTGGAAGAGGCTTTAATTACGATGAATAAATAAGATCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCCAAGTCaaaaaa                                                      >  1:236596/1‑141 (MQ=255)

                                                                                     cgaTGCATCTTGATGAAATGACTGTGGAAGAGGCTTTAATTACGATGAATAAATAAGATCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAgg                                                    <  1:492179/141‑1 (MQ=255)

                                                                                        tgcatttcgcggaAATGACTGTGGAAGAGTCTTTAATTACGATGAATCAATAAGACAAGCAAGTCACGTTAGCAGTGCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTg                                                 <  1:211690/130‑1 (MQ=255)

                                                                                        tGCACCGTGATGAAATGACTGGGGAAGAGGCTTTAATTACGATGAATAAATAAGATCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAAGACCACAATTGCCAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTg                                                 <  1:375419/141‑1 (MQ=255)

                                                                                         gCACCTGGATGGAATGACTGTGGAAGAGGCTTTAATTACAATGAATAAATAAGATCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAACTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTgg                                                <  1:485357/141‑1 (MQ=255)

                                                                                           aTCTTGATGAAATGACTGTGGAAGAGGCTTTAATTACGATGAATAAATAAGATCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACACAATAAAAAGGGGGGAc                                              >  1:234844/1‑141 (MQ=255)

                                                                                                             tgGAAGAGGCTTTAATTACGATGAATAAATAAGATCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTg                            >  1:286655/1‑141 (MQ=255)

                                                                                                                    ggCTTTAATTACGATGAATAAATAAGATCAGCAAGTCCCGTTAGCAGTTCGGAAGGCAATACCACTATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTCGACGATTGATTTATATCGGTGCAGGTAc                     >  1:345162/1‑141 (MQ=255)

                                                                                                                       tttAATTACGATGAATAAATAAAAACCACTAGGCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGCAGGTACGAg                  >  1:365830/1‑141 (MQ=255)

                                                                                                                              aCGATGAATAAATAAGATCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGCAGGTACAAGTGGAAgg           >  1:374072/1‑141 (MQ=255)

                                                                                                                                     ataaataaGATCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTTATTTATATCGGTGCAGGTACAAGTGGAAGGTTGGgtg    >  1:11885/1‑141 (MQ=255)

                                                                                                                                     ataaataaGATCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGCAGGTACAAGTGGAGGGTTGGgtg    >  1:450399/1‑141 (MQ=255)

                                                                                                                                       aaaTAAGATCAGCAAGTCCCGTTAGCAGTTCGGAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGGCGATTGAGTTATATCGGTGCAGGTACAAGTGGAAGGTTGGgtgta  >  1:104929/1‑140 (MQ=255)

GCCGAATGATACATTTGATTTTCAAAAAACTAGGGAGTGTAAGAAGTGATGGAAAATAGTACGACCGAAGCGCGTAATGAAGCGACGATGCATCTTGATGAAATGACTGTGGAAGAGGCTTTAATTACGATGAATAAAGAAGATCAGCAAGTCCCGTTAGCAGTTCGAAAGGCAATACCACAATTGACAAAAGTAATTAAAAAAACAATTGCACAGTATAAAAAGGGTGGACGATTGATTTATATCGGTGCAGGTACAAGTGGAAGGTTGGGTGTC  >  CP000730/224866‑225141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A