Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A15 F59 I1 R1	25	48.3	927160	99.3%	920669	151.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	2,342,380	A→C	L78V (TTG→GTG)	USA300HOU_2199 ←	possible HAD superfamily hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,342,380	0	A	C	100.0%	187.4 / NA	49	L78V (TTG→GTG)	USA300HOU_2199	possible HAD superfamily hydrolase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (25/24); total (25/24)

TTCGACATTATATTGCGCAGAAATATGACGTAATATATCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCAAATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGTGATCACTTCATTATTATTAGTTAGTTTTTCCAACAATTTTTCCATTTCTCTTA  >  CP000730/2342240‑2342526

ttCGACATTATATTGCGCAGAAATATGACGTAATATATCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAg                                                                                                                                          <  2:217843/151‑1 (MQ=255)

ttCGACATTATATTGCGCAGAAATATGACGTAATATATCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAg                                                                                                                                          <  2:227541/151‑1 (MQ=255)

 tCGACATTATATTGCGCAGAAATATGACGTAATATATCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGa                                                                                                                                         <  2:220244/151‑1 (MQ=255)

 tCGACATTATATTGCGCAGAAATATGACGTAATATATCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGa                                                                                                                                         <  2:362447/151‑1 (MQ=255)

  cGACATTATATTGCGCAGAAATATGACGTAATATATCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGat                                                                                                                                        <  1:313294/151‑1 (MQ=255)

        tataTTGCGCAGAAATATGACGTAATATATCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTaa                                                                                                                                  >  2:132658/1‑151 (MQ=255)

          tatTGCGCAGAAATATGACGTAATATATCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATg                                                                                                                                >  2:441579/1‑151 (MQ=255)

           atTGCGCAGAAATATGACGTAATATATCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATgg                                                                                                                               >  2:419645/1‑151 (MQ=255)

           atTGCGCAGAAATATGACGTAATATATCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATgg                                                                                                                               >  2:187716/1‑151 (MQ=255)

           atTGCGCAGAAATATGACGTAATATATCTTCAATAACTTCAAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCTCATCAGAAAAGATATGTAATgg                                                                                                                               >  2:419839/1‑151 (MQ=255)

               cgcAGAAATATGACGTAATATATCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTa                                                                                                                           >  2:255372/1‑151 (MQ=255)

                gcAGAAATATGACGTAATATATCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTaa                                                                                                                          >  1:145525/1‑151 (MQ=255)

                gcAGAAATATGACGTAATATATCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGGAAGGGTTTaa                                                                                                                          >  1:193185/1‑151 (MQ=255)

                       tatGACGTAATATATCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCat                                                                                                                   <  1:102365/151‑1 (MQ=255)

                         tGACGTAATATATCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCatat                                                                                                                 >  2:196352/1‑151 (MQ=255)

                         tGACGTAATATATCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCatat                                                                                                                 >  1:450925/1‑151 (MQ=255)

                             gTAATATATCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAAc                                                                                                             <  1:306067/151‑1 (MQ=255)

                                  atatcttcaatatcttcaAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGAt                                                                                                        <  1:36605/151‑1 (MQ=255)

                                   tatcttcaatatcttcaaATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATc                                                                                                       >  1:224962/1‑151 (MQ=255)

                                    atcttcaatatcttcaaATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATcc                                                                                                      >  1:236827/1‑151 (MQ=255)

                                                    aaTAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGacac                                                                                      >  2:439731/1‑151 (MQ=255)

                                                        aaaaTGATTCACTCATCACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGCTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTg                                                                                  <  2:25071/151‑1 (MQ=255)

                                                         aaaTGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGa                                                                                 <  1:382533/151‑1 (MQ=255)

                                                            tGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAAt                                                                              >  1:25633/1‑151 (MQ=255)

                                                                   cTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTc                                                                       <  2:399274/151‑1 (MQ=255)

                                                                         aCTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCa                                                                 >  1:224428/1‑151 (MQ=255)

                                                                          ctttctttTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCaa                                                                >  1:247287/1‑151 (MQ=255)

                                                                          ctttctttTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCaa                                                                <  1:91356/151‑1 (MQ=255)

                                                                          ctttctttTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCaa                                                                <  1:381693/151‑1 (MQ=255)

                                                                                ttttATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATAcagc                                                          <  2:157042/151‑1 (MQ=255)

                                                                                ttttATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATAcagc                                                          <  2:147061/151‑1 (MQ=255)

                                                                                   tataTGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGt                                                       >  1:213607/1‑151 (MQ=255)

                                                                                    atatGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGTg                                                      <  2:226908/151‑1 (MQ=255)

                                                                                        gTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGTGATCa                                                  >  2:199352/1‑151 (MQ=255)

                                                                                        gTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGTGATCa                                                  >  2:71474/1‑151 (MQ=255)

                                                                                        gTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGTGATCa                                                  >  2:233726/1‑151 (MQ=255)

                                                                                         tttCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGTGATCAc                                                 <  2:47433/151‑1 (MQ=255)

                                                                                          ttCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGTGATCACt                                                >  2:124280/1‑151 (MQ=255)

                                                                                                 tCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGTGGTCACTTCattat                                         <  1:285806/151‑1 (MQ=255)

                                                                                                 tCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGTGATCACTTCattat                                         <  1:332939/151‑1 (MQ=255)

                                                                                                                    tCCAAGTGAACATTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGTGATCACTTCATTATTATTAGTTAGTTTTTCcaa                      >  2:236854/1‑151 (MQ=255)

                                                                                                                               aTTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGTGATCACTTCATTATTATTAGTTAGTTTTTCCAACAATTTTTCCa           <  1:181735/151‑1 (MQ=255)

                                                                                                                               aTTTTAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGTGATCACTTCATTATTATTAGTTAGTTTTTCCAACAATTTTTCCa           <  1:199081/151‑1 (MQ=255)

                                                                                                                                   tAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGTGATCACTTCATTATTATTAGTTAGTTTTTCCAACAATTTTTCCATTtc       >  2:185955/1‑151 (MQ=255)

                                                                                                                                   tAGAACTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTCGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGTGATCACTTCATTATTATTAGTTAGTTTTTCCAACAATTTTTCCATTtc       >  2:71001/1‑151 (MQ=255)

                                                                                                                                      aaCTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGTGATCACTTCATTATTATTAGTTAGTTTTTCCAACAATTTTTCCATTTctct    <  1:254005/151‑1 (MQ=255)

                                                                                                                                      aaCTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGTGATCACTTCATTATTATTAGTTAGTTTTTCCAACAATTTTTCCATTTctct    <  1:216583/151‑1 (MQ=255)

                                                                                                                                      aaCTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGTGATCACTTCATTATTATTAGTTAGTTTTTCCAACAATTTTTCCATTTctct    <  1:263138/151‑1 (MQ=255)

                                                                                                                                        cTCACATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGTGATCACTTCATTATTATTAGTTAGTTTTTCCAACAATTTTTCCATTTCTCTTa  >  1:314835/1‑151 (MQ=255)

TTCGACATTATATTGCGCAGAAATATGACGTAATATATCTTCAATATCTTCAAATAAAAATGGTTCGCTCAAGACTTTCTTTTTATATGTTTCTGATTCAACATATTCGCCATTATTCCAAGTGAACATTTTAGAACTCAAATCAGAAAAGATATGTAATGGTTTAAACGTCATATTAACTTGATCCATTTTATCCATGACACTTTGAAATGTACTTCCTGTCAATACAGCAGTGATCACTTCATTATTATTAGTTAGTTTTTCCAACAATTTTTCCATTTCTCTTA  >  CP000730/2342240‑2342526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A