Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A23 F20 I0 R1 92 76.5 1681216 93.1% 1565212 141.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,929,925 T→A 55.6% intergenic (‑1008/‑160) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,929,9250TA55.6% 48.5 / 158.0 126intergenic (‑1008/‑160)USA300HOU_1794/USA300HOU_1796hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (24/32);  new base A (36/34);  total (60/66)
Fisher's exact test for biased strand distribution p-value = 3.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.81e-01

Only 100 of 132 total aligned reads displayed.

GCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTAATTATACT  >  CP000730/1929785‑1930061
                                                                                                                                                |                                                                                                                                               
gCTTGGATATTGGCCGATGATAGAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGtt                                                                                                                                                 <  2:199097/141‑1 (MQ=255)
  ttGTATATTCACAGATGGTGTAATAATAGAGTCGCCTATCTCTCAGGCGTCAACT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatta                                                                                                                                              >  2:511428/1‑140 (MQ=255)
   tGTTTATTTGCCGAGGATGTAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGttttt                                                                                                                                              <  2:836436/141‑1 (MQ=255)
    gTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGtttttt                                                                                                                                             <  1:704293/141‑1 (MQ=255)
     tataTTCACAGATGGTATAATAATAGATTCGCCTATCTTTAAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatac                                                                                                                                           <  2:278859/141‑5 (MQ=255)
       tattcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCAAATCATAGCACCAGTCATCAGTGCCTGTGCTGTTGCGtattatactt                                                                                                                                         >  2:298755/9‑135 (MQ=255)
       tattcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACtt                                                                                                                                         >  2:55213/9‑141 (MQ=255)
       tattcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACtt                                                                                                                                         >  1:618788/9‑141 (MQ=255)
       tatTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCt                                                                                                                                          <  2:255223/141‑1 (MQ=255)
         ttcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAt                                                                                                                                       >  2:810716/7‑141 (MQ=255)
         ttcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGGGGCTGTGCTGTTGCGTATTATACTTAt                                                                                                                                       >  1:143126/7‑141 (MQ=255)
           cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTg                                                                                                                                     >  1:790995/5‑141 (MQ=255)
           cacagatggtAGAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTg                                                                                                                                     >  2:616332/5‑141 (MQ=255)
             cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc                                                                                                                                   <  1:413275/139‑1 (MQ=255)
             cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc                                                                                                                                   <  1:563988/139‑1 (MQ=255)
              agatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt                                                                                                                                  <  1:786131/140‑1 (MQ=255)
               gatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATCATTCTTGGCtt                                                                                                                                 >  2:752379/1‑140 (MQ=255)
               gatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtt                                                                                                                                 >  1:48936/1‑140 (MQ=255)
               aatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑TTTGCTAATCATTTTCGTTCACGTCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtt                                                                                                                                 <  2:50584/140‑2 (MQ=255)
                atgatATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTa                                                                                                                                 <  1:490140/141‑1 (MQ=255)
                 tgttataAGAATAGAGTCGCCTAACTCTCCGGCGTCAATT‑‑GACGAAGAGAGGAGGTTCA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAg                                                                                                                               <  2:303127/138‑1 (MQ=255)
                 tggtatcATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GCCGAAGAGATGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttag                                                                                                                               <  2:570768/138‑4 (MQ=255)
                 tggtataATACTAGATGCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttag                                                                                                                               <  2:756122/138‑4 (MQ=255)
                 tgatATAATAGTAGAGTCGCCTATATCTCAAGCTTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTaa                                                                                                                                >  2:347823/1‑141 (MQ=255)
                 gggtataAGAGTAGAGTCGCCTATCTTTCGTGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGTACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAg                                                                                                                               <  2:498392/138‑1 (MQ=255)
                  ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                                                                                              <  1:361531/139‑1 (MQ=255)
                  ggtataATAATAGAGTCGCCTATCCCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCTCATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                                                                                              <  1:241552/139‑1 (MQ=255)
                   gtataATAGGGGGGGCGCCTATCTCTCAGGCGTGAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGt                                                                                                                              <  2:191874/140‑1 (MQ=255)
                   gtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑TGCTAATCATTTTCGTTCACATGATAGCACCAGTCATCAGTGGATGTGCTGTTGCGTATTATACTTATTGGCttggta                                                                                                                             >  2:197150/2‑136 (MQ=255)
                   atatAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGt                                                                                                                              >  2:41776/1‑141 (MQ=255)
                   atatAATAGTAGAGTCGACTCTCTAAAGGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGt                                                                                                                              >  2:385095/1‑141 (MQ=255)
                    tataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaa                                                                                                                            <  2:233635/141‑2 (MQ=255)
                    tataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaa                                                                                                                            >  2:115692/1‑140 (MQ=255)
                     ataataataGAGTCGCCTATCTCTCCGGTGTAAA‑‑TGGACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTCTACTTATGGGCtttgtaaa                                                                                                                           <  2:800948/141‑8 (MQ=255)
                       aataatAGAGTTGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACg                                                                                                                         >  1:307755/1‑141 (MQ=255)
                        ataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                                                                                                        <  1:810716/141‑1 (MQ=255)
                         taatagAGTCGCCTATCTCTCAGGCCTCAATT‑‑GAAGAAGAGAGGTGGTGCATTAC‑T‑T‑TGTTAATCATTTTCCTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa                                                                                                                       <  2:379340/138‑1 (MQ=255)
                          aatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaa                                                                                                                      <  1:111478/139‑1 (MQ=255)
                          aatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaa                                                                                                                      >  2:650488/3‑141 (MQ=255)
                          aatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCCTAGTAAACGCaa                                                                                                                      >  2:39152/3‑141 (MQ=255)
                           atatagTCCCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaac                                                                                                                     <  1:107199/137‑2 (MQ=255)
                             agagTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATa                                                                                                                    >  2:836573/1‑141 (MQ=255)
                              gagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaa                                                                                                                  >  2:642553/1‑137 (MQ=255)
                              gagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaa                                                                                                                  <  1:376056/141‑5 (MQ=255)
                              gagTCGCCGATGTGTCAGGCTGCAATCGAGGCGCAGAGGGGAGGTGTA‑TAA‑G‑G‑GGATGCTTATTTTCGTTCACATCATAGCTCCAGTCATTAGTGGCTGTGCCAGTGCGTTTTTGTCTGATGGGCTAAGTAGACGCAATAc                                                                                                                   <  2:171833/141‑1 (MQ=255)
                               agTCGCCTATCTGTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCAGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaat                                                                                                                 >  1:592453/1‑136 (MQ=255)
                               agTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCAAATCATAGCACCAGTCATCAGTGGCTGTGCGGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaact                                                                                                                 >  2:90573/1‑136 (MQ=255)
                               agTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaat                                                                                                                 >  2:198806/1‑136 (MQ=255)
                                gTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGGTGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑aa                                                                                                               >  2:325926/1‑141 (MQ=255)
                                gTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCAACAa‑‑aa‑‑a                                                                                                            >  1:13125/1‑141 (MQ=255)
                                gTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑TTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaata                                                                                                                <  2:347035/141‑7 (MQ=255)
                                 tCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAGTAC‑‑aa‑‑a                                                                                                            >  1:26459/1‑141 (MQ=255)
                                   gCCTGTCTCTCAGGCGTCAATT‑‑GGCGAGGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCCTTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                                                                                             <  2:133413/141‑10 (MQ=255)
                                   gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                                                                                             <  2:568671/141‑10 (MQ=255)
                                    ccTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatta                                                                                                            <  1:814002/141‑11 (MQ=255)
                                    ccTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCCTAGTAAACGCaccaaataatta                                                                                                            >  1:191735/1‑130 (MQ=255)
                                     cTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAGTCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaa                                                                                                           <  2:571334/141‑12 (MQ=255)
                                     cTAGCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaa                                                                                                           <  1:775842/141‑12 (MQ=255)
                                     atATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTACTAAACGCaccaaataattaa                                                                                                           >  2:514129/2‑129 (MQ=255)
                                      tATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaat                                                                                                          <  2:59972/141‑13 (MQ=255)
                                      tATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGATGTGCTGTTGCTTATTATACTTATTGGCTTAGTAAACGCaacaaataattaat                                                                                                          >  1:781601/1‑129 (MQ=255)
                                      tATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑TTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaat                                                                                                          <  2:42152/141‑13 (MQ=255)
                                      tATCTCTCAGGCGTCAA‑‑TAGACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaat                                                                                                          <  1:735220/141‑13 (MQ=255)
                                       ttctctcAGGCGTCAATTTAGACGCAGAGCGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGt                                                                                                      <  1:230311/140‑1 (MQ=255)
                                       aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTCATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                                                                                                         >  2:131690/1‑128 (MQ=255)
                                       aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                                                                                                         >  1:417073/1‑128 (MQ=255)
                                             cAGGCGTTGATTTAGACGAAGAATTTAGTTTTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ata                                                                                               <  2:625527/141‑1 (MQ=255)
                                               ggCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTCTTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGAGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑Atat‑a                                                                                            >  1:788125/1‑141 (MQ=255)
                                                        ttAGCCGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCa                                                                                  <  1:331499/141‑1 (MQ=255)
                                                        ttAGCCGCAGAGAGGAGGTGTA‑CAA‑G‑G‑TGCTGCTTATTTTCGAAAGAGTCATCGTTTATGGCCCCGATCGCTGTGCCATTGCGTTTTGTGCTAGTTTTCCAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCa                                                                                  <  2:804414/141‑1 (MQ=255)
                                                              gCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATaaaa                                                                            <  2:576682/141‑1 (MQ=255)
                                                              gCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATaaaa                                                                            <  2:515932/141‑1 (MQ=255)
                                                                     ggaggTGTA‑TAA‑G‑G‑TGATGCTGATTTTCGTTCGCATCATAGAACCAGTAATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCt                                                                     <  2:610102/141‑1 (MQ=255)
                                                                       aggTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTTTTTTTTGTTTAGTAGACGCAATAC‑‑AA‑‑AATGGGTGAC‑ATAT‑ACCC‑GCGCAAGTACAAATCCCCTcc                                                                   >  2:787829/1‑140 (MQ=255)
                                                                        tgtgtA‑TAA‑C‑G‑TTATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCAc                                                                  <  1:52103/140‑1 (MQ=255)
                                                                        ggTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCAc                                                                  <  1:347823/141‑1 (MQ=255)
                                                                                aa‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTCCCGCa                                                           >  2:22551/1‑141 (MQ=255)
                                                                                         aTGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGt                                                     >  2:522931/1‑141 (MQ=255)
                                                                                             ttATTTTGGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAgg                                                 <  2:144275/141‑1 (MQ=255)
                                                                                             ttATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAgg                                                 <  2:686996/141‑1 (MQ=255)
                                                                                              tagtttCGTTCACATCATAGCACCAGTCATCAGTGGTTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAggg                                                <  2:136150/138‑1 (MQ=255)
                                                                                              tATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAggg                                                >  2:229317/1‑141 (MQ=255)
                                                                                               aTTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCCATAAAAATCCCCTCACTACCGGAAATAGTGAgggg                                               <  2:720683/141‑1 (MQ=255)
                                                                                                ttttCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATGGTGAggggg                                              >  1:669930/1‑140 (MQ=255)
                                                                                                ttttCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGa                                              <  2:292295/141‑1 (MQ=255)
                                                                                                ttttCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGa                                              <  1:314793/141‑1 (MQ=255)
                                                                                                           atcatAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATaa                                   <  2:713905/141‑1 (MQ=255)
                                                                                                            tcatAGCACCAGTCATCAGTGGCTGTGCCATTGCGTATTTTTCTTATTGGCTTAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑CCACCAATAAAAATCCCCTCACTACCCACAATAGCGAGGGGATTGGTTTACaaa                                  >  2:146387/1‑140 (MQ=255)
                                                                                                              atAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCAACAAATAATTAATAGGCGACTTTATAAGCCAGCATCGA‑AAAAATCCCCACACTACTGC‑AATAGTGTGGGGATTGGTGcat                                     >  1:26559/1‑141 (MQ=255)
                                                                                                                 gCACCAGTCGGCACTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAAt                             <  1:603952/141‑1 (MQ=255)
                                                                                                                  cACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCGCCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATa                            >  1:653156/1‑141 (MQ=255)
                                                                                                                  cACCAGTCATCAGTGCCTGTGCCATTGCGTTTTTTTCATATTGCCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGAGATTGGTGTATAAGTAAATa                            <  1:589259/141‑1 (MQ=255)
                                                                                                                  cACCAATCATCACGGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTAAAGCAATCGATTATGTTATTGGTGTATAAGTAAATa                            <  1:405245/141‑1 (MQ=255)
                                                                                                                     cAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACtt                         <  2:658926/141‑1 (MQ=255)
                                                                                                                      aGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGGGAGGGGAGTGGTGTATAAGTAAATACttt                        >  1:548900/1‑140 (MQ=255)
                                                                                                                         catcaGTTGGTGTTCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTAttt                     <  2:564504/141‑1 (MQ=255)
                                                                                                                                   tgtgCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTg               >  2:260277/1‑137 (MQ=255)
                                                                                                                                   tgtgCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCtt           <  1:385095/141‑1 (MQ=255)
                                                                                                                                    gtgCCATTGCGTTTCTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTa          >  1:546464/1‑141 (MQ=255)
                                                                                                                                            gCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTAATTATACt  >  2:595940/1‑141 (MQ=255)
                                                                                                                                                |                                                                                                                                               
GCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTAATTATACT  >  CP000730/1929785‑1930061

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A