Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F21 I0 R1 100 98.5 2221552 92.3% 2050492 141.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,929,941 A→T 49.2% intergenic (‑1024/‑144) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,929,9410AT49.2% 14.8 / 168.1 123intergenic (‑1024/‑144)USA300HOU_1794/USA300HOU_1796hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (35/26);  new base T (37/23);  total (74/49)
Fisher's exact test for biased strand distribution p-value = 7.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

Only 100 of 126 total aligned reads displayed.

ATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑GG‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAAT‑‑ACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTAATTATACTGCTAATTTTTCTTTTTG  >  CP000730/1929801‑1930078
                                                                                                                                                |                                                                                                                                                
atgatCTCAGAGGAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑GG‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTa                                                                                                                                                  <  2:1054650/141‑1 (MQ=255)
atgatATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑GG‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTa                                                                                                                                                  >  1:118213/1‑141 (MQ=255)
 tggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑TT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGGTGTGCTGTTGCGTATTATACTTATTGGCTTAg                                                                                                                                                >  1:334817/4‑141 (MQ=255)
 tggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑TT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAg                                                                                                                                                >  2:19058/4‑141 (MQ=255)
 tggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑TT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAg                                                                                                                                                >  2:115399/4‑141 (MQ=255)
  ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑TT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                                                                                                               >  1:515389/3‑141 (MQ=255)
  ggtaaaataaaagagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑TT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                                                                                                               <  2:300352/130‑1 (MQ=255)
  gatATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑GG‑TGATGCTTATTTTCGTTCACATCGGAGGCCGAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAg                                                                                                                                                >  1:306137/1‑141 (MQ=255)
  gatATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑GG‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAg                                                                                                                                                >  1:857951/1‑141 (MQ=255)
  gatATAATAGTAGAGTCGCCTATCTATCCGGCGTCAATTTAGACGCAGAGAGGAGGGG‑TA‑TAA‑GG‑TGATGCTTATTTTCGTTCACATCATAGCGCCAGTCATCCGTGGCTGTGCCAGTGCGTTTTTTTCTGGTGGGCTAAg                                                                                                                                                >  1:527547/1‑141 (MQ=255)
   gtataATAATAGAGTCGGCTATCTCTCAGGGGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑TT‑TGCTAATCATTTTCGTTCACATCATAGCGCCAGTCATCAGTGGCTGTGCTGTTGCGTGTTATACTTATTGGTTTAGTa                                                                                                                                              >  2:256610/2‑141 (MQ=255)
   gtataATAATAGAGTCGCCTATCTCTCAGGCGTCGATT‑‑GACGAAGAGAGGAGCTG‑CATTAC‑TT‑TGCTAATAATTTTCGTTCACATCATAGCACCAATCATCAGTGGCTGTGCTGTTGCGTATTATATGTATTGGCTTAGTa                                                                                                                                              >  2:348131/2‑141 (MQ=255)
   gtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑TT‑TGCTAATCATTTTCGTTCACCTCATATCACCAGTCATCAGTGGCGGTGCTGTTGCGTATTATACTTATTGGCTTAGTa                                                                                                                                              >  2:260405/2‑141 (MQ=255)
   gtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑TT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTa                                                                                                                                              >  1:800885/2‑141 (MQ=255)
   gtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑TT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTa                                                                                                                                              >  1:132719/2‑141 (MQ=255)
   gtataATAATAGAGTCGCCTAGCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑TT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCCTACGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTa                                                                                                                                              >  1:571971/2‑141 (MQ=255)
    tataATAATAGAGTCGCCTATCTCTCAGGCGTCACTT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑TT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTAATCAGTGGCTGTGCTGTTGCGTATTATCCTTATTGGCTTAGTaa                                                                                                                                             >  2:896836/1‑140 (MQ=255)
    tataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑TT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaa                                                                                                                                             >  1:869104/1‑140 (MQ=255)
    tataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑TT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaa                                                                                                                                             <  2:768041/141‑2 (MQ=255)
    tataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑TT‑TGCTAATCATTTTCGTTCACATCAGAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaa                                                                                                                                             >  1:915620/1‑140 (MQ=255)
    tataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑TT‑TGCTAATCATTTTCGTTCAAATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATCCTTATTGGCTTAGtca                                                                                                                                             >  2:518844/1‑139 (MQ=255)
    tataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGACGAGGTG‑CATTAC‑TT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaa                                                                                                                                             >  2:714008/1‑140 (MQ=255)
     ataacaATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑TT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaa                                                                                                                                            <  2:518142/141‑3 (MQ=255)
      taataaTAGATTCTCCTATCTCCCGGGCGTCAATT‑‑GACGAAGAGAGGACGTGCCT‑TAC‑TT‑TGCTTCTCATTTTCGTTCACATCATAGCACCATTCATCAGTTGCTGTGCTGTTGCGTATTATTCTTATTGGCTTAGTAAAc                                                                                                                                           <  2:115887/141‑1 (MQ=255)
       aaatatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACTAAGAGAGGAGGTG‑CATTAC‑TT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACg                                                                                                                                          <  2:816243/136‑1 (MQ=255)
        ataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑TT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                                                                                                                         <  1:966583/141‑1 (MQ=255)
        ataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACTT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                                                                                                                         <  2:581846/141‑1 (MQ=255)
         tagtagAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑GG‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGc                                                                                                                                         <  1:216377/141‑1 (MQ=255)
         tagtagAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑GG‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGc                                                                                                                                         <  1:1004251/141‑1 (MQ=255)
         tagtagAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑GG‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGc                                                                                                                                         <  1:1071881/141‑1 (MQ=255)
          aatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑TT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                                                                                                                         <  2:646883/137‑1 (MQ=255)
          aatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑TT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                                                                                                                         >  1:646883/3‑139 (MQ=255)
           atagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑TT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaac                                                                                                                                      >  2:1066255/2‑140 (MQ=255)
           atagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACTT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGCAAACGCaac                                                                                                                                      >  2:851496/2‑140 (MQ=255)
            tagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑TT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaaca                                                                                                                                     <  1:280341/141‑3 (MQ=255)
            tagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑TT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaaca                                                                                                                                     <  1:571420/141‑3 (MQ=255)
            aagagTCGCCTATCTCTCATGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACTT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCacca                                                                                                                                     <  2:927093/140‑4 (MQ=255)
             agagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACTT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaa                                                                                                                                    >  2:595827/1‑138 (MQ=255)
               agTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑TT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTGTACTTCTTGGCTTAGTAAACGccacaaat                                                                                                                                  >  2:359396/1‑134 (MQ=255)
                 tCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑GG‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAGGTCGCCGCAAT‑‑ACaa‑‑a                                                                                                                             >  1:315166/1‑141 (MQ=255)
                 cagCCTATCTCTCAATCCTCAATTTATACGCAGAGAGGAGGTG‑TA‑TAA‑GG‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTCCTTATTGGCTAAGTAGACGCAAT‑‑ACaa‑‑a                                                                                                                             <  1:467949/139‑1 (MQ=255)
                  cGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑GG‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAAT‑‑ACaa‑‑aa                                                                                                                            <  2:201777/141‑1 (MQ=255)
                   gCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑AA‑TAA‑GG‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAAT‑‑ACaa‑‑aaa                                                                                                                           >  2:551636/1‑140 (MQ=255)
                   gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑TT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                                                                                                              <  1:741885/141‑10 (MQ=255)
                   gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑CTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                                                                                                              <  2:526243/141‑10 (MQ=255)
                    ccTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑GG‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGTCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCATT‑‑ACAA‑‑AATa                                                                                                                          >  1:610897/1‑141 (MQ=255)
                    ccTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACTT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacagctaatta                                                                                                                             >  1:953996/1‑131 (MQ=255)
                    ccTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACTT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatta                                                                                                                             >  2:748463/1‑131 (MQ=255)
                     cTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑GG‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAAT‑‑ACAA‑‑AATAg                                                                                                                         <  1:285229/141‑1 (MQ=255)
                     cTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑GG‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAAT‑‑ACAA‑‑AATAg                                                                                                                         <  1:603150/141‑1 (MQ=255)
                     cTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑TT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaa                                                                                                                            >  2:870350/1‑130 (MQ=255)
                     cTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑TT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaa                                                                                                                            >  2:318013/1‑130 (MQ=255)
                     cTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑TT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataagtaa                                                                                                                            >  2:742861/1‑130 (MQ=255)
                     cTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑TT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataagtaa                                                                                                                            >  2:742876/1‑130 (MQ=255)
                      tATCTCTCAGGCTTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑TT‑TGCTAATCATTTTCGTTCACATCATAGCGCCAGTCATTATTGGCTGTGCTGTGGCTTATTATACTTATTGGCTTAGTAAACGCaacaaataattaat                                                                                                                           <  2:1064077/141‑13 (MQ=255)
                      tATCTCTCAGGCGTCAATT‑‑GACGAAGGGAGGAGGTG‑CATTAC‑TT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaat                                                                                                                           <  2:510179/141‑13 (MQ=255)
                      tATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑TT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGCTGCGTATTATACTGATGGTCTTAGTAAACGCaacaaataatcaat                                                                                                                           >  2:882173/1‑129 (MQ=255)
                       aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑TT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTATACGCaacaaataattaata                                                                                                                          <  1:607034/141‑14 (MQ=255)
                       aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑TT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                                                                                                                          >  1:361686/1‑128 (MQ=255)
                       aTCTCTCACGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACTT‑TGCTAATGATTTTCGTTCACATCATAGCACCAGTCATCAGTGGGTGTGCTGTTGCGTATTATACTTAGTGGCTTAGTAAACGCaacaaataattaata                                                                                                                          >  2:815114/1‑128 (MQ=255)
                        tctctcAGGCTTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑TT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaatag                                                                                                                         <  2:681465/141‑15 (MQ=255)
                         ctctcAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑TT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCAAC‑‑ACAT‑‑AAttaatagg                                                                                                                    >  2:340398/1‑134 (MQ=255)
                         ctctcAGGCGTCAATATAGACGCAGAGAGGAGGTG‑TA‑TAA‑GG‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCCAT‑‑ACAA‑‑AATAGGTGa                                                                                                                     >  1:820861/1‑141 (MQ=255)
                                  gTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑GG‑TGATGTTTATCTTCGTTCAAATCCTATCTTCCGTGATCAGTGTCTGTGCTATCGCGTTTTTTACTTATTGTCTTACTAACCGCAAT‑‑ACAA‑‑AATAGGTGAC‑ATAT‑GGcc                                                                                                          <  2:458261/141‑1 (MQ=255)
                                   tCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑GG‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCAATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAAT‑‑ACAA‑‑AATCGGTGAC‑ATAT‑AGCC‑g                                                                                                        >  1:1002530/1‑141 (MQ=255)
                                                     ggaggTG‑TA‑TAA‑GG‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAAT‑‑ACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCt                                                                                      <  2:857951/141‑1 (MQ=255)
                                                     ggaggTG‑TA‑TAA‑GG‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCCGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAAT‑‑ACAA‑‑AATCGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCt                                                                                      <  2:729976/141‑1 (MQ=255)
                                                      gaggTG‑TA‑TAA‑GG‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAAT‑‑ACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTc                                                                                     <  1:1101970/141‑1 (MQ=255)
                                                       aggTG‑TA‑TAA‑GG‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAAT‑‑ACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCa                                                                                    >  2:903794/1‑141 (MQ=255)
                                                        ggTG‑TA‑TAA‑GG‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAAT‑‑ACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCAc                                                                                   <  1:1035769/141‑1 (MQ=255)
                                                         cgataa‑taA‑GG‑AAATGCTGGTCTGCGTTCACATCAATGCGCCCCTCATCAGCGGCTGCGCCTTTTCGTTTTCTTCTTACCGGCAAACTATACGCAAT‑‑AGAA‑‑AATATGTGAC‑ATAT‑AGCT‑TCACCAATAAAAATCCCCTCAc                                                                                   <  2:857658/136‑1 (MQ=255)
                                                           g‑tA‑TAA‑GG‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTTGCTAAGTAGACTCAAT‑‑ACAG‑‑AATAGGTGAC‑ATAT‑AGAC‑GAACCAATAAAAATCCCCTGAcgaa                                                                                >  1:194561/1‑138 (MQ=255)
                                                                taA‑GG‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAAT‑‑ACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGc                                                                             >  2:632019/1‑141 (MQ=255)
                                                                 aa‑GG‑TAATGCTTGTTTGCGTCCACATCCTAGCACCAGTCATCAGTGGCTTTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAAT‑‑ACAA‑‑AATAGGTGAC‑ACAT‑AGCC‑GCACCAATAACAATCCCCTCACTACCGCa                                                                            <  2:213763/141‑1 (MQ=255)
                                                                  a‑GG‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTTTCTCAGTAGACTCTAT‑‑ACGA‑‑ATTAGTGGCC‑ATAT‑ATCC‑GCCCCAATAAAAATCCCCTCACTACCGCaa                                                                           >  2:231315/1‑141 (MQ=255)
                                                                  a‑GG‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAAT‑‑ACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCaa                                                                           >  1:861554/1‑141 (MQ=255)
                                                                  a‑GG‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAAT‑‑ACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCaa                                                                           >  2:331773/1‑141 (MQ=255)
                                                                  a‑GG‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAAT‑‑ACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCaa                                                                           <  1:951174/141‑1 (MQ=255)
                                                                       tGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGGTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAAT‑‑ACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GAAACAATAAAAATCCCCTCACTACCGCAAATa                                                                        <  2:118213/141‑1 (MQ=255)
                                                                       tGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAAT‑‑ACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATa                                                                        <  1:719582/141‑1 (MQ=255)
                                                                        gATGCTGATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAAT‑‑ACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAg                                                                       <  1:390148/141‑1 (MQ=255)
                                                                             ttATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAAT‑‑ACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAgg                                                                  >  2:988190/1‑141 (MQ=255)
                                                                             ttATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAAT‑‑ACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAAAAGTGAgg                                                                  >  1:1028616/1‑141 (MQ=255)
                                                                              tactttCGTTCACATCATCGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTGTTTCTTATTGGCTAAGTAGACGCAAT‑‑ACAA‑‑AATAGGCGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAggg                                                                 <  2:279408/138‑1 (MQ=255)
                                                                               aTTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAAT‑‑ACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAgggg                                                                >  1:840814/1‑141 (MQ=255)
                                                                                    cGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAAT‑‑ACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTgg                                                           >  1:444873/1‑141 (MQ=255)
                                                                                    cGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAAT‑‑ACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTgg                                                           >  2:778228/1‑141 (MQ=255)
                                                                                    cGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAAT‑‑ACAA‑‑AATAGGTGAC‑AGAT‑AGCC‑GCACCAATAAAAATCCCCTCCCTACCGCAAAAAGTGAGGGGATTgg                                                           >  1:21586/1‑141 (MQ=255)
                                                                                       tCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAAT‑‑ACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGgtgt                                                        >  1:622748/1‑141 (MQ=255)
                                                                                       tCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAAT‑‑ACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGgtgt                                                        >  2:183488/1‑141 (MQ=255)
                                                                                               tAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCAACAAATAATTAATAGGCGACTTTATAAGCCAGCATCGA‑AAAAATCCCCCCACTACTGC‑AATAGTGTGGGGATTGGTGcatt                                                     >  1:216538/1‑140 (MQ=255)
                                                                                                 gCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAAT‑‑ACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAAt                                              <  1:342894/141‑1 (MQ=255)
                                                                                                    ccAGTCATACATGGCTGTTCCATTTCGTTTTTTTCTTATTGGCTAAGTAGACGCAAT‑‑ACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTGAAGACt                                           >  2:1072782/1‑141 (MQ=255)
                                                                                                          atcaGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAAT‑‑ACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTAtttt                                     >  1:651664/1‑141 (MQ=255)
                                                                                                                 gCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAAT‑‑ACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTc                              <  2:840814/141‑1 (MQ=255)
                                                                                                                         aTTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAAT‑‑ACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTAATtat                      <  1:874452/141‑1 (MQ=255)
                                                                                                                           tGCGTTTTTTTCTTATTGGCTAAGTAGACGCAAT‑‑ACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTAATTATAc                    <  1:778228/141‑1 (MQ=255)
                                                                                                                                ttttttCTTATTGGCTAAGTAGACGCAAT‑‑ACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTAATTATACTGCTa               <  2:861554/141‑1 (MQ=255)
                                                                                                                                        tATTGGCTAAGTAGACGCAAT‑‑ACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTAATTATACTGCTAATTTTTCt       >  2:334082/1‑141 (MQ=255)
                                                                                                                                             gCTAAGTAGACGCAAT‑‑ACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTAATTATACTGCTAATTTTTCtttttt  >  1:345864/1‑140 (MQ=255)
                                                                                                                                                |                                                                                                                                                
ATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑GG‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAAT‑‑ACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTAATTATACTGCTAATTTTTCTTTTTG  >  CP000730/1929801‑1930078

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A