Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F67 I1 R1	13	62.5	1286300	92.5%	1189827	135.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	2,722,719	C→T	G13D (GGT→GAT)	cobW3 ←	cobalamin (vitamin B12) biosynthesis protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,722,719	0	C	T	100.0%	183.5 / NA	53	G13D (GGT→GAT)	cobW3	cobalamin (vitamin B12) biosynthesis protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (29/24); total (29/24)

TCAGTTCACTTAAAGGTATGTCTTCAGATACTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTACCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGATCTCCTATTGACTGACATGACTCTATATTCAGTTATAGTT  >  CP000730/2722582‑2722845

tCAGTTCACTTAAAGGTATGTCTTCAGATACTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGc                                                                                                                             <  1:107472/141‑1 (MQ=255)

  aGTTCACTTAAAGGTATGTCTTCAGATACTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCa                                                                                                                           <  1:42839/141‑1 (MQ=255)

   gTTCACTTAAAGGTATGTCTTCAGATACTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCAc                                                                                                                          >  1:514424/1‑141 (MQ=255)

        cTTAAAGGTATGTCTTCAGATACTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTaaa                                                                                                                     <  2:230359/141‑1 (MQ=255)

             aGGTATGTCTTCAGATACTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAAccc                                                                                                                >  2:282065/1‑141 (MQ=255)

                  tGTCTTCAGATACTAAGGCACCATCAACACTCATTTTCCCATATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCta                                                                                                           >  2:199919/1‑141 (MQ=255)

                  tGTCTTCAGATACTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCta                                                                                                           >  1:92160/1‑141 (MQ=255)

                  tGTCTTCAGATACTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCta                                                                                                           >  1:587263/1‑141 (MQ=255)

                     cTTCAGATACTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTataa                                                                                                        <  2:18356/141‑1 (MQ=255)

                     cTTCAGATACTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTataa                                                                                                        <  2:206221/141‑1 (MQ=255)

                     cTTCAGATACTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTataa                                                                                                        <  2:21774/141‑1 (MQ=255)

                      ttCAGATACTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTataat                                                                                                       <  1:295991/141‑1 (MQ=255)

                            tACTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTAt                                                                                                 <  1:191498/141‑1 (MQ=255)

                               tAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTAtttt                                                                                              >  1:95848/1‑141 (MQ=255)

                               tAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTAtttt                                                                                              >  1:600232/1‑141 (MQ=255)

                               tAAGGCACCATCAACACNCATTTTCCCANATTCNTTCNTGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCACCACCTAAAAACCCACCTATAATAACTAtttt                                                                                              >  1:394922/1‑141 (MQ=255)

                                    cACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCt                                                                                         <  2:365809/141‑1 (MQ=255)

                                      ccATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGAAAAATAAAAACCCACCTATAATAACTATTTTCATCTGc                                                                                       <  2:519561/141‑1 (MQ=255)

                                          cAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTAAGCGATTCCTTTAATGACTAAGAGAGCATATGACTTAAGACAGTCGTTATTTAGTAATTAATAAAAAGCCCTATAATAACTATTTTCATCTGCGAct                                                                                   >  1:94480/1‑141 (MQ=255)

                                           aaCACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGActc                                                                                  <  2:587263/141‑1 (MQ=255)

                                               cTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCCTCTGCGACTctcct                                                                              >  1:134744/1‑141 (MQ=255)

                                                   ttttCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTta                                                                          >  2:330489/1‑141 (MQ=255)

                                                   ttttCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTta                                                                          <  1:281544/141‑1 (MQ=255)

                                                       cccAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTctcc                                                                               >  1:579710/1‑132 (MQ=255)

                                                       cccAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTctcc                                                                               <  2:579710/132‑1 (MQ=255)

                                                       cccAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACt                                                                      >  1:200784/1‑141 (MQ=255)

                                                        ccAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACtt                                                                     <  1:639664/141‑1 (MQ=255)

                                                                  tcatGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATa                                                           >  1:559908/1‑141 (MQ=255)

                                                                        tgaCTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCatat                                                     <  2:407900/141‑1 (MQ=255)

                                                                          aCTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTc                                                   >  1:129569/1‑141 (MQ=255)

                                                                           ctgctgGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTcc                                                  >  2:332595/1‑141 (MQ=255)

                                                                                   ttCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCACCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAagataga                                          <  2:394922/141‑1 (MQ=255)

                                                                                      aGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAagata                                            <  1:3143/136‑1 (MQ=255)

                                                                                      aGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAagata                                            >  2:3143/1‑136 (MQ=255)

                                                                                         gATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGATCTCCt                                    <  1:170422/141‑1 (MQ=255)

                                                                                             ccTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGATCTCCTATtg                                >  1:219011/1‑141 (MQ=255)

                                                                                              cTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGATCTCCTATtga                               <  2:129569/141‑1 (MQ=255)

                                                                                               tttAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGATCTCCTATtgac                              >  1:523109/1‑141 (MQ=255)

                                                                                                        tCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGATCTCCTATTGACTGACATGAc                     >  2:189611/1‑141 (MQ=255)

                                                                                                        tCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGATCTCCTATTGACTGACATGAc                     >  2:90005/1‑141 (MQ=255)

                                                                                                        tCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGATCTCCTATTGACTGACATGAc                     >  2:90016/1‑141 (MQ=255)

                                                                                                        tCAGCGAGCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGATCTCCTATTGACTGACATGAc                     >  2:90076/1‑141 (MQ=255)

                                                                                                               gCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATCATAACTATTTTCATCTGCGCCTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGATCTCCTATTGACTGACATGACTCTatat              <  1:569173/141‑1 (MQ=255)

                                                                                                               gCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGATCTCCTATTGACTGACATGACTCTatat              <  2:200784/141‑1 (MQ=255)

                                                                                                               gCAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGATCTCCTATTGACTGACATGACTCTatat              >  2:625810/1‑141 (MQ=255)

                                                                                                                cAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGATCTCCTATTGACTGACATGACTCta                 >  2:380379/1‑137 (MQ=255)

                                                                                                                cAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGATCTCCTATTGACTGACATGACTCta                 <  1:380379/137‑1 (MQ=255)

                                                                                                                cAAATGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGATCTCCTATTGACTGACATGACTCTATAtt             <  2:594180/141‑1 (MQ=255)

                                                                                                                 aaaTGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGATCTCCTATTGACTGACATGACTCTATATTc            >  1:507220/1‑141 (MQ=255)

                                                                                                                   aTGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGATCTCCTATTGACTGACATGACTCTATATTCAg          <  1:330489/141‑1 (MQ=255)

                                                                                                                   aTGATTTAAGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATACATCTCCTATTGACTGACATGACTCTATATTCAg          >  2:121869/1‑141 (MQ=255)

                                                                                                                          aaGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGATCTCCTATTGACTGACATGACTCTATATTCAGTTATAGt   >  2:137047/1‑141 (MQ=255)

                                                                                                                           aGACAGTCGTTTTATCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGATCTCCTATTGACTGACATGACTCTATATTCAGTTATAGtt  >  1:624237/1‑141 (MQ=255)

TCAGTTCACTTAAAGGTATGTCTTCAGATACTAAGGCACCATCAACACTCATTTTCCCAAATTCATTCATGATGACTGCTGGTTTCAGCGATTCCTTTAATGATTCAGCGAGCAAATGATTTAAGACAGTCGTTTTACCGCCACCTAAAAACCCACCTATAATAACTATTTTCATCTGCGACTCTCCTCTTATACTTCATATGAATAGCATATTCCAAGATAGATCTCCTATTGACTGACATGACTCTATATTCAGTTATAGTT  >  CP000730/2722582‑2722845

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A