Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A19 F106 I1 R1 24 58.2 1363720 92.2% 1257349 135.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP000730 2,455,699 A→T H406L (CAT→CTT)  USA300HOU_2315 → hypothetical membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,455,6990AT95.5% 183.3 / ‑4.4 67H406L (CAT→CTT) USA300HOU_2315hypothetical membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/3);  new base T (31/32);  total (31/36)
Fisher's exact test for biased strand distribution p-value = 2.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAATTTTA  >  CP000730/2455570‑2455826
                                                                                                                                 |                                                                                                                               
ttAAACAAACATCACAAAGTGGCCAATTAACAAATGATGCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTAATGCAAAAAAAAATATCg                                                                                                                            <  2:80886/135‑1 (MQ=255)
    aCAAGCACCACAAAGTTACCATTTAACAAACGATTCAAAAGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATTCA‑AAAAAATTATCGaaaaa                                                                                                                       <  1:356226/135‑1 (MQ=255)
    aCAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAA‑TTATCGaaaaa                                                                                                                       <  1:112169/135‑1 (MQ=255)
    aCAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGaaaaa                                                                                                                       >  1:455959/1‑135 (MQ=255)
    aCAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGaaaaa                                                                                                                       >  2:389099/1‑135 (MQ=255)
    aCAAGCACCACAAAGTGACGAATTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGaaaaa                                                                                                                       >  1:242420/1‑135 (MQ=255)
        gCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAA‑TTATCGAAAAAACTga                                                                                                                  >  1:204743/1‑135 (MQ=255)
          accacAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTgaa                                                                                                                 >  1:82224/1‑135 (MQ=255)
           tcacaATGTGATGATGAAACAAACGATTCAAGTGTAGATGAAATAGAGCAAGCATTAAAAGAACCTGCGACGTATAAAGAAGACAGACGTTCATCATTTTTAATTGATGCA‑AAAAAATTTTCGTAAATACTgaag                                                                                                                <  2:82227/134‑1 (MQ=255)
             acaAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAA‑TTATCGACAAAACTgaagaa                                                                                                              >  2:38932/1‑135 (MQ=255)
             acaAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAA‑TTAACGAAAAAACTgaagaa                                                                                                              >  2:279171/1‑135 (MQ=255)
                aaGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATTGAAAAAACTGAAGAACaa                                                                                                           <  1:22861/135‑1 (MQ=255)
                aaGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACaa                                                                                                           >  1:672745/1‑135 (MQ=255)
                 aGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAAt                                                                                                          <  1:40463/135‑1 (MQ=255)
                 aGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAAAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAA‑TTATCGAAAAAACTGAAGAACAAt                                                                                                          >  1:666582/1‑135 (MQ=255)
                   tgccGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTAAAGAACAATCt                                                                                                        >  1:593897/4‑135 (MQ=255)
                   tGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAA‑TTATCGAAAAAACTGAAGAACAATCt                                                                                                        >  1:497132/1‑135 (MQ=255)
                   tGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCt                                                                                                        <  1:168279/135‑1 (MQ=255)
                      cGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCa                                                                                                     >  1:646169/1‑135 (MQ=255)
                        aTTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGa                                                                                                   >  2:600153/1‑135 (MQ=255)
                                    aTTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCTACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACaaaga                                                                                       <  1:610605/135‑3 (MQ=255)
                                      tCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAATACAGACGGTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAaataat                                                                                     <  2:156387/135‑1 (MQ=255)
                                         aaTGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAAGAATAAc                                                                                  >  2:615457/1‑135 (MQ=255)
                                                 tGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCTAAAAATCTGTAGAACAATTTTCAGATAAAAACAAATAATAACAAAGCGCt                                                                          <  1:9343/135‑1 (MQ=255)
                                                            aaTCATTAAAAGAACCGGCGACTTATAAATAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACct                                                               <  2:480103/135‑1 (MQ=255)
                                                              tctttAAAAGAACCTGCGACTTATAAAGAAAACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACctct                                                             <  2:35615/132‑1 (MQ=255)
                                                              tCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGATGTAATTGATGCAAAAAAA‑TTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTGGCTAGTACcact                                                             >  2:400576/1‑135 (MQ=255)
                                                              tCATGAAAAGAACCTGCTACTTCTAAAGAAGACAGACGTTAATCAGGTCTAATTGATGCA‑AAAAAATTAACGAAAAAACTGAAGAATAATCTTCAGATAAAAACAAATAGTAACAAAGCGCTTGCTAGTACctct                                                             <  2:350166/135‑1 (MQ=255)
                                                               cATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAA‑TTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTGGCTAGTACCTCtt                                                            >  2:573350/1‑135 (MQ=255)
                                                               cATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAGTAACAAAGCGCTCGCTAGCACCTCtt                                                            >  2:388377/1‑135 (MQ=255)
                                                               cATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCCCATGCTAGTACCTCtt                                                            >  2:141048/1‑135 (MQ=255)
                                                                  tAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCATGCTAGTACCTCTTaaa                                                         >  2:265828/1‑135 (MQ=255)
                                                                    aaaGAACCTGCGACTTATAGAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAA‑TTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAACGCGCTTGCTAGTACCTCTTaaaaa                                                       >  2:157476/1‑135 (MQ=255)
                                                                    aaaGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAGAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTaaaaa                                                       >  2:470133/1‑135 (MQ=255)
                                                                    aaaGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAgcatcatcttttta                                                       <  1:279171/135‑14 (MQ=255)
                                                                    aaaGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTacaaa                                                       >  2:300521/1‑135 (MQ=255)
                                                                    aaaGAACCTGCGACATATAAAGCAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAAAAAAAAAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTaaaaa                                                       <  2:123871/135‑1 (MQ=255)
                                                                     aaGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAg                                                      >  2:389654/1‑135 (MQ=255)
                                                                      aGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAA‑TTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAgat                                                    <  2:204743/135‑1 (MQ=255)
                                                                       gAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAgat                                                    <  2:175406/135‑1 (MQ=255)
                                                                        atccTGCGACTTATCAAGAAGACAGATGTTCATCAGCTGTAACTGATGCAAAAAAA‑TTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAATGCGCTTGCTAGTCCCTATTAAAAAgatg                                                   <  1:428839/133‑1 (MQ=255)
                                                                        aaCCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCATATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAgatg                                                   <  2:544164/135‑1 (MQ=255)
                                                                        aaCCTGCGACTTATAAAGAAGACAGACGTTCATCAGTCGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACACAGCGCTTGCTAGTACCTCTTAAAAAgatg                                                   <  2:372562/135‑1 (MQ=255)
                                                                        aaCCTGCGACTTAAAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACTAATCGCTTTTTAGTACCTCTTAAAAAgatg                                                   <  1:491885/135‑1 (MQ=255)
                                                                         aCCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAgatga                                                  <  1:96766/135‑1 (MQ=255)
                                                                             gctaCTTATAAAGACGACAGACGGTCATCAGTTGTAA‑ACCCAAAAACAAAAAAATCGAAAAAACCGAAGAACAATCTTCAGATAAAAACAAATAAGAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCt                                              <  2:429063/132‑1 (MQ=255)
                                                                              cGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTa                                             >  2:465952/1‑135 (MQ=255)
                                                                                 cttcgtaggaagaCAGATGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATACTAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCa                                          <  2:319175/127‑1 (MQ=255)
                                                                                 cTTATAAAGAAGAGAGACGTTCATCAGTTGTAATTTATGCAAAAAAA‑TTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATTTCAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCa                                          <  1:525442/135‑1 (MQ=255)
                                                                                  ttATAAAGAAGCCAGACGTTCATCAGTTGTAATTTATGCA‑AAAAAATTATCGAAAAATCTGAAGAACAATCTTCAGATAAAAACAAATAATAACTAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCaa                                         <  1:81105/135‑1 (MQ=255)
                                                                                  ttATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAA‑TTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCaa                                         >  1:100725/1‑135 (MQ=255)
                                                                                     taAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACACAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAgcg                                      <  2:638918/135‑1 (MQ=255)
                                                                                     aaccgtagccagatGTTCATCAGCTGTAATTGATGCA‑AAAAAATGATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAgcg                                      <  2:239680/126‑1 (MQ=255)
                                                                                      aaaGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAGATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAgcgc                                     <  1:342078/135‑1 (MQ=255)
                                                                                       aggaagtCAGACGTTCATCAGTTGTAATTGAGGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCt                                    <  2:338484/133‑1 (MQ=255)
                                                                                       acgaagaCAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCt                                    >  2:45911/3‑135 (MQ=255)
                                                                                            gaccgacGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTct                               >  1:188919/1‑135 (MQ=255)
                                                                                               aggcGTTCATCAGTTGGAATTGATGCA‑AAAAAATTATCGAAAAAACTGAATAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCACGCGCTTTTctata                            <  2:227803/132‑1 (MQ=255)
                                                                                               agacGTTCATCAGTTGTAATTGATGCAAAAAAA‑TTATCGAAAAAACTGAATAACAATCTTCAGATACATACAAATAATAACTGTGGGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCGAGCGCTTTTctata                            <  1:129565/135‑1 (MQ=255)
                                                                                                 acGTTCATCAGTTTTACTGGATGCA‑AAAAAAGTATCGCAAAAACTGAAGAACAAGCTTCAGATAAAAACAAATAGTAACAGAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCCCGCGCTTTTctatact                          <  2:398185/135‑1 (MQ=255)
                                                                                                 acGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTCGAATGCGCCTTTctatact                          >  2:582107/1‑135 (MQ=255)
                                                                                                  cGTTCATCAGTCGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTctatacta                         <  2:82224/135‑1 (MQ=255)
                                                                                                    ttCATCAGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTctatactata                       >  1:421432/1‑135 (MQ=255)
                                                                                                      catctGTTGTAATTAATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACtatatc                     >  1:202630/1‑134 (MQ=255)
                                                                                                      catcaGTTGTAATTGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACtatata                     >  1:434669/1‑135 (MQ=255)
                                                                                                                   tGATGCA‑AAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTaaa        <  1:600153/135‑1 (MQ=255)
                                                                                                                          aaaaaaaTTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAATTTTa  <  1:515777/134‑1 (MQ=255)
                                                                                                                                 |                                                                                                                               
TTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAATTTTA  >  CP000730/2455570‑2455826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A