Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A27 F34 I0 R2	578	165.1	5883352	98.2%	5777451	141.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,543,231	A→C	6.5%	M277R (ATG→AGG) ‡	narU ←	nitrate/nitrite transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,543,231	0	A	C	6.5%	541.0 / 24.1	154	M277R (ATG→AGG) ‡	narU	nitrate/nitrite transporter
Reads supporting (aligned to +/- strand): ref base A (72/72); new base C (2/8); total (74/80)
Fisher's exact test for biased strand distribution p-value = 1.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.71e-01

Only 100 of 159 total aligned reads displayed.

CATAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATGC  >  NC_000913/1543083‑1543379

cATAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCa                                                                                                                                                       >  1:15320/1‑149 (MQ=255)

 aTAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTTGCCAGCa                                                                                                                                                       >  4:571790/1‑149 (MQ=255)

 aTAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCAt                                                                                                                                                      >  2:706042/1‑149 (MQ=255)

 aTAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCAt                                                                                                                                                      >  3:1318487/1‑149 (MQ=255)

  tAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATg                                                                                                                                                     <  3:1431647/149‑1 (MQ=255)

  tAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATg                                                                                                                                                     >  1:659338/1‑149 (MQ=255)

     aaaTAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑a                                                                                                                                                 >  1:618973/1‑149 (MQ=255)

      aaTAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCATGCTGGC‑aa                                                                                                                                                >  3:784930/1‑149 (MQ=255)

        tAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑aaaa                                                                                                                                              <  2:618973/149‑1 (MQ=255)

        tAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑‑TTTTGCCATGCTGGC‑aaaaa                                                                                                                                             <  2:134372/149‑2 (MQ=255)

         aaaGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCATGCTGGC‑aaaaa                                                                                                                                             <  4:784930/149‑2 (MQ=255)

         aaaGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCATGCTGGC‑aaaaa                                                                                                                                             <  1:238393/149‑2 (MQ=255)

            gTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑‑TTTTGCCATGCTGGC‑AAAAaccca                                                                                                                                         <  4:1098635/149‑3 (MQ=255)

             ttGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCGCCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGc                                                                                                                                         >  2:962609/1‑149 (MQ=255)

             ttGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGc                                                                                                                                         <  1:1097886/149‑1 (MQ=255)

             ttGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGc                                                                                                                                         >  1:1367679/1‑149 (MQ=255)

             ttGATCAACGTCACCCGCACGCCACCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGc                                                                                                                                         <  3:1314691/149‑1 (MQ=255)

                  cAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGaaa                                                                                                                                    <  2:1300603/149‑1 (MQ=255)

                   aaCGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGaaaa                                                                                                                                   >  1:486329/1‑149 (MQ=255)

                    aCGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAAccc                                                                                                                                           <  1:1363504/140‑1 (MQ=255)

                      gTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAAcc                                                                                                                                >  4:748098/1‑149 (MQ=255)

                      gTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAAcc                                                                                                                                >  2:923421/1‑149 (MQ=255)

                         aCCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGAt                                                                                                                             >  3:167628/1‑149 (MQ=255)

                            cGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGa                                                                                                                              <  1:214885/145‑1 (MQ=255)

                                cgccgcCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGaa                                                                                                                      <  4:610515/149‑1 (MQ=255)

                                 gccgccGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAAc                                                                                                                     <  3:193140/149‑1 (MQ=255)

                                       gAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAgg                                                                                                               <  1:1192196/149‑1 (MQ=255)

                                       gAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAgg                                                                                                               <  2:807928/149‑1 (MQ=255)

                                        aaCTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGt                                                                                                              <  3:151678/149‑1 (MQ=255)

                                         aCTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACAAACCGAAGGTg                                                                                                             <  3:962052/149‑1 (MQ=255)

                                           ttATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGc                                                                                                           >  2:1173096/1‑149 (MQ=255)

                                              tCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAg                                                                                                        >  1:853960/1‑149 (MQ=255)

                                                 gAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTa                                                                                                     >  4:739490/1‑149 (MQ=255)

                                                 gAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTa                                                                                                     >  3:371218/1‑149 (MQ=255)

                                                 gAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTa                                                                                                     >  4:1165860/1‑149 (MQ=255)

                                                    aTAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAg                                                                                                  <  4:1328553/149‑1 (MQ=255)

                                                     tAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGc                                                                                                 <  3:748098/149‑1 (MQ=255)

                                                      aGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCa                                                                                                >  1:1409117/1‑149 (MQ=255)

                                                       gCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAg                                                                                               >  4:809994/1‑149 (MQ=255)

                                                        caccacCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAgg                                                                                              >  2:1123757/1‑149 (MQ=255)

                                                         accaccAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGc                                                                                             >  1:1222846/1‑149 (MQ=255)

                                                          ccaccaACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCt                                                                                            >  2:685965/1‑149 (MQ=255)

                                                            accaACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTca                                                                                          <  2:486329/149‑1 (MQ=255)

                                                              caACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTcagc                                                                                        >  3:711920/1‑149 (MQ=255)

                                                               aaCCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTcagca                                                                                       >  4:1415303/1‑149 (MQ=255)

                                                                  cgaccgCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGcc                                                                                    <  4:1307425/149‑1 (MQ=255)

                                                                    accgCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCag                                                                                  <  2:763372/149‑1 (MQ=255)

                                                                     ccgCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCaga                                                                                 <  2:721663/149‑1 (MQ=255)

                                                                       gcgcgATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAgaga                                                                               <  2:437057/149‑1 (MQ=255)

                                                                        cgcgATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGAt                                                                              <  3:410264/149‑1 (MQ=255)

                                                                                cACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGt                                                                      <  2:265173/149‑1 (MQ=255)

                                                                                 aCCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGtt                                                                     >  1:202359/1‑149 (MQ=255)

                                                                                  ccGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTg                                                                    >  2:656768/1‑149 (MQ=255)

                                                                                  ccGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTg                                                                    >  1:554781/1‑149 (MQ=255)

                                                                                  ccGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTg                                                                    <  3:172363/149‑1 (MQ=255)

                                                                                   cGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCagag                                                                                >  1:860495/1‑136 (MQ=255)

                                                                                   cGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGt                                                                   <  4:1093520/149‑1 (MQ=255)

                                                                                    gATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATaaa                                                                                                                          <  1:1157276/93‑1 (MQ=255)

                                                                                    gATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATaaa                                                                                                                          >  2:1157276/1‑93 (MQ=255)

                                                                                    gATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTa                                                                  >  2:810483/1‑149 (MQ=255)

                                                                                    gATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTa                                                                  >  4:339388/1‑149 (MQ=255)

                                                                                     aTAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTaa                                                                 <  2:1367679/149‑1 (MQ=255)

                                                                                     aTAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTaa                                                                 <  2:222984/149‑1 (MQ=255)

                                                                                     aTAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGAATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTaa                                                                 <  4:1181305/149‑1 (MQ=255)

                                                                                           gggCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAgg                                                           <  4:159067/149‑1 (MQ=255)

                                                                                             gCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTg                                                                                                             <  2:205969/97‑1 (MQ=255)

                                                                                             gCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTg                                                                                                             >  1:205969/1‑97 (MQ=255)

                                                                                              ccAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAg                                                        >  1:1363102/1‑149 (MQ=255)

                                                                                              ccAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAg                                                        <  1:270113/149‑1 (MQ=255)

                                                                                               cAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGc                                                       <  3:284776/149‑1 (MQ=255)

                                                                                                aaaGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCt                                                      >  2:606284/1‑149 (MQ=255)

                                                                                                 aaGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAgg                                                           >  1:782507/1‑143 (MQ=255)

                                                                                                  aGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTgg                                                    >  1:1381766/1‑149 (MQ=255)

                                                                                                    aaCGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTc                                                  <  1:1271434/149‑1 (MQ=255)

                                                                                                     aCGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAGCGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCg                                                 >  1:1066545/1‑149 (MQ=255)

                                                                                                     aCGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCg                                                 >  4:1142828/1‑149 (MQ=255)

                                                                                                       gCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTaa                                                                 <  4:1051734/131‑1 (MQ=255)

                                                                                                       gCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTaa                                                                 >  3:1051734/1‑131 (MQ=255)

                                                                                                         cAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCaa                                             >  1:479114/1‑149 (MQ=255)

                                                                                                             cgcAGAATATTGACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAATCCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCaa                                             >  1:1207054/1‑145 (MQ=255)

                                                                                                             cgcAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGa                                         <  2:936337/149‑1 (MQ=255)

                                                                                                             cgcAAAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCaa                                             <  2:1207054/145‑1 (MQ=255)

                                                                                                              gcAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAg                                        <  2:1381766/149‑1 (MQ=255)

                                                                                                              gcAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAg                                        >  4:1047127/1‑149 (MQ=255)

                                                                                                                aGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGgc                                      <  4:367544/149‑1 (MQ=255)

                                                                                                                 gAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGgcg                                     >  2:66953/1‑149 (MQ=255)

                                                                                                                 gAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGgc                                      <  3:1141462/148‑1 (MQ=255)

                                                                                                                  aaTATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGgcgc                                    >  3:1055693/1‑149 (MQ=255)

                                                                                                                   atatTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGCGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGcgcg                                   >  3:30769/1‑149 (MQ=255)

                                                                                                                              cGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGc                          >  2:120103/1‑147 (MQ=255)

                                                                                                                                    cTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATAtcat                  <  3:718067/149‑1 (MQ=255)

                                                                                                                                       gg‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGC‑‑GCGTTGTAAGACAGGGAGc                                                       >  3:433684/1‑107 (MQ=255)

                                                                                                                                        gggttttGCCATGCTGGCAAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAg                                                        <  3:151807/106‑1 (MQ=255)

                                                                                                                                        g‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATAtcattcat              >  1:147105/1‑149 (MQ=255)

                                                                                                                                          tttttGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATc             <  3:1142828/149‑1 (MQ=255)

                                                                                                                                          tttttGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATc             <  4:145491/149‑1 (MQ=255)

                                                                                                                                          tttttGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATc             <  2:124416/149‑1 (MQ=255)

                                                                                                                                           ttttGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATcc            <  4:785578/149‑1 (MQ=255)

                                                                                                                                            tttGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATccc           <  1:517924/149‑1 (MQ=255)

                                                                                                                                                     aTGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATgc  <  4:451878/149‑1 (MQ=255)

CATAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATGC  >  NC_000913/1543083‑1543379

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A