Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A14 F68 I0 R1 683 78.3 2772122 98.0% 2716679 141.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,543,231 A→C 5.9% M277R (ATG→AGG) ‡ narU ← nitrate/nitrite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,543,2310AC 5.9% 231.8 / 6.1 69M277R (ATG→AGG) ‡narUnitrate/nitrite transporter
Reads supporting (aligned to +/- strand):  ref base A (32/30);  new base C (2/2);  total (35/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

CATAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCA‑GCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATG  >  NC_000913/1543083‑1543378
                                                                                                                                                     |                                                                                                                                                   
cATAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCA‑GCa                                                                                                                                                     <  2:655083/149‑1 (MQ=255)
 aTAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCA‑GCAt                                                                                                                                                    >  2:272949/1‑149 (MQ=255)
  tAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCA‑GCATg                                                                                                                                                   >  2:340890/1‑149 (MQ=255)
  tAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCA‑GCATg                                                                                                                                                   <  3:607460/149‑1 (MQ=255)
        tAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCA‑GCATGGCaaaa                                                                                                                                             >  4:268202/1‑149 (MQ=255)
         aaaGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCATGC‑TGGCaaaaa                                                                                                                                            <  1:128206/149‑2 (MQ=255)
            gTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCA‑GCATgg                                                                                                                                                  >  4:546621/1‑140 (MQ=255)
            gTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCA‑GCATgg                                                                                                                                                  <  3:546621/140‑1 (MQ=255)
               gATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCA‑GCATGGCAAAACCCGCAg                                                                                                                                      >  4:622709/1‑149 (MQ=255)
                aTCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCA‑GCATGGCAAAACCCGCAGa                                                                                                                                     >  2:168300/1‑149 (MQ=255)
                      gTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCA‑GCATGGCAAAACCCGCAGAAAAAcc                                                                                                                               >  2:102167/1‑149 (MQ=255)
                           ccGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCA‑GCATGGCAAAACCCGc                                                                                                                                        <  1:51243/135‑1 (MQ=255)
                           ccGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCA‑GCATGGCAAAACCCGc                                                                                                                                        >  2:51243/1‑135 (MQ=255)
                           ccGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCA‑GCATGGCAAAACCCGCAGAAAAACCGATaa                                                                                                                          >  3:630842/1‑149 (MQ=255)
                             acACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGAACGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGTAACTGGGTTTTTGCCA‑GCATGGCAAAACCCGCAGAAAAACCGATAAAc                                                                                                                        >  4:225135/2‑149 (MQ=255)
                                     ccgAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCA‑GCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGaa                                                                                                                >  1:100360/1‑149 (MQ=255)
                                                 gAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCA‑GCATGGCAAAACCCGCAGAAAAACCGATaaa                                                                                                                         >  1:176621/1‑128 (MQ=255)
                                                 gAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCA‑GCATGGCAAAACCCGCAGAAAAACCGATaaa                                                                                                                         <  2:176621/128‑1 (MQ=255)
                                                 gAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCA‑GCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTa                                                                                                    >  1:33465/1‑149 (MQ=255)
                                                    aTAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCA‑GCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAg                                                                                                 >  1:95588/1‑149 (MQ=255)
                                                     tAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCA‑GCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGc                                                                                                <  3:115344/149‑1 (MQ=255)
                                                       gCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCA‑GCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAg                                                                                              <  3:135499/149‑1 (MQ=255)
                                                        caccacCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCA‑GCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAgg                                                                                             <  1:144753/149‑1 (MQ=255)
                                                          ccaccaACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCA‑GCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCt                                                                                           >  2:155807/1‑149 (MQ=255)
                                                           caccaACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCA‑GCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTc                                                                                          >  4:641357/1‑149 (MQ=255)
                                                            accaACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTGCCA‑TGCTGGCaaaaac                                                                                                                                           <  1:168158/98‑3 (MQ=255)
                                                            accaACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTGCCA‑TGCTGGCaaaaac                                                                                                                                           >  2:168158/1‑96 (MQ=255)
                                                                  cgaccgCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCA‑GCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGcc                                                                                   <  1:302649/149‑1 (MQ=255)
                                                                   gaccgCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCA‑GCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTaaa                                                                                                  <  4:468879/133‑1 (MQ=255)
                                                                   gaccgCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCA‑GCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTaaa                                                                                                  >  3:468879/1‑133 (MQ=255)
                                                                   gaccgCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCA‑GCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCa                                                                                  >  2:469980/1‑149 (MQ=255)
                                                                           gATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCA‑GCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAgaga                                                                              >  2:355465/1‑145 (MQ=255)
                                                                           gATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCA‑GCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAgaga                                                                              <  1:355465/145‑1 (MQ=255)
                                                                             tGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCA‑GCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAgg                                                                        <  1:168300/149‑1 (MQ=255)
                                                                                 aCCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCA‑GCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGtt                                                                    <  2:100360/149‑1 (MQ=255)
                                                                                       aaaTGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCATGC‑TGGCaaaaa                                                                                                                                            <  3:462678/71‑2 (MQ=38)
                                                                                       aaaTGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCATGC‑TGGCaaaaa                                                                                                                                            >  4:462678/1‑70 (MQ=38)
                                                                                         aTGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCA‑GCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACa                                                            <  2:33465/149‑1 (MQ=255)
                                                                                           gggCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCA‑GCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAgg                                                          <  3:268202/149‑1 (MQ=255)
                                                                                           gggCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCA‑GCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAgg                                                          <  2:95588/149‑1 (MQ=255)
                                                                                             gCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCA‑GCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGa                                                        >  2:224121/1‑149 (MQ=255)
                                                                                              ccAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCA‑GCATGGCACAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAg                                                       >  3:406502/1‑149 (MQ=255)
                                                                                              ccAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCA‑GCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGt                                                                  >  2:68747/1‑138 (MQ=255)
                                                                                              ccAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCA‑GCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGt                                                                  <  1:68747/138‑1 (MQ=255)
                                                                                               cAAAGAACGCCAGGCGCAGAATATTGACATCCGGGAACTGGGTTTTTGCCA‑GCATGGCAAAACCCGCAGGAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTaa                                                                >  3:198802/1‑139 (MQ=255)
                                                                                               cAAAGAACGCCAGGCGCAGAATATTGACATCCGGGAACTGGGTTTTTGCCA‑GCATGGCAAAACCCGCAGGAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTaa                                                                <  4:198802/139‑1 (MQ=255)
                                                                                                aaaGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCA‑GCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCt                                                     >  1:5460/1‑149 (MQ=255)
                                                                                                 aaGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCA‑GCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGAc                                                             >  4:585675/1‑140 (MQ=255)
                                                                                                 aaGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCA‑GCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGAc                                                             <  3:585675/140‑1 (MQ=255)
                                                                                                           ggCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCA‑GCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAAtt                                          <  1:224121/149‑1 (MQ=255)
                                                                                                                aGAATATTCACATCCGGGAACTGGGTTTTTGCCA‑GCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGgc                                     >  3:628826/1‑149 (MQ=255)
                                                                                                                  aaTATTCACATCCGGGAACTGGGTTTTTGCCA‑GCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGgcgc                                   <  1:340890/149‑1 (MQ=255)
                                                                                                                  aaTATTCACATCCGGGAACTGGGTTTTTGCCA‑GCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGgcgc                                   <  1:155807/149‑1 (MQ=255)
                                                                                                                    tatTCACATCCGGGAACTGGGTTTTTGCCA‑GCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGt                                 <  1:469980/149‑1 (MQ=255)
                                                                                                                    tatTCACATCCGGGAACTGGGTTTTTGCCA‑GCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGt                                 >  4:268901/1‑149 (MQ=255)
                                                                                                                         acaTCCGGGAACTGGGTTTTTGCCA‑GCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACt                            >  3:352223/1‑149 (MQ=255)
                                                                                                                            tCCGGGAACTGGGTTTTTGCCA‑GCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGc                         <  2:5460/149‑1 (MQ=255)
                                                                                                                              cGGGAACTGGGTTTTTGCCA‑GCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGa                       <  1:102167/149‑1 (MQ=255)
                                                                                                                              cGGGAACTGGGTTTTTGCCA‑GCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGa                       <  3:622709/149‑1 (MQ=255)
                                                                                                                                 gAACTGGGTTTTTGCCA‑GCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGatat                    <  4:628826/149‑1 (MQ=255)
                                                                                                                                     tGGGTTTTTGCCA‑GCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATAtcatt                >  1:256867/1‑149 (MQ=255)
                                                                                                                                     tGGGTTTTTGCCA‑GCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATAtcatt                >  1:552802/1‑149 (MQ=255)
                                                                                                                                     tGGGTTTTTGCCA‑GCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATAtcat                 >  2:283965/1‑148 (MQ=255)
                                                                                                                                     tGGGTTTTTGCCA‑GCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATAtcat                 <  1:283965/148‑1 (MQ=255)
                                                                                                                                       gggttttGCCA‑TGCTGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGtt                                                                    <  4:122110/92‑1 (MQ=255)
                                                                                                                                       gggttttGCCA‑TGCTGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGtt                                                                    >  3:122110/4‑95 (MQ=255)
                                                                                                                                        gTTTTTGCCA‑GCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATAtcattcat             <  4:630842/149‑1 (MQ=255)
                                                                                                                                         tttttGCCA‑GCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATc            <  1:272949/149‑1 (MQ=255)
                                                                                                                                                cA‑GCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGAcc     >  2:82549/1‑149 (MQ=255)
                                                                                                                                                    cATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATg  <  3:641357/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CATAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCA‑GCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATG  >  NC_000913/1543083‑1543378

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A