Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A16 F70 I0 R1
|
629 |
86.3 |
3068034 |
98.1% |
3009741 |
142.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
899,656 |
T→C |
7.3% |
intergenic (+11/+188) |
rlmC → / ← artJ |
23S rRNA m(5)U747 methyltransferase, SAM‑dependent/arginine ABC transporter periplasmic binding protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 899,656 | 0 | T | C | 7.3%
| 169.4
/ 3.7
| 55 | intergenic (+11/+188) | rlmC/artJ | 23S rRNA m(5)U747 methyltransferase, SAM‑dependent/arginine ABC transporter periplasmic binding protein |
| Reads supporting (aligned to +/- strand): ref base T (20/31); new base C (2/2); total (22/33) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.70e-01 |
TACTCCAGCTGTAACGCCCAAACTATGGCGAAAGATATCCGCGAACTGCCTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATT > NC_000913/899508‑899797
|
tACTCCAGCTGTAACGCCCAAACTATGGCGAAAGATATCCGCGAACTGCCTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCAt > 3:656743/1‑149 (MQ=255)
cTCCAGCTGTAACGCCCAAACTATGGCGAAAGATATCCGCGAACTGCCTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCAtgt < 4:742661/149‑1 (MQ=255)
cTCCAGCTGTAACGCCCAAACTATGGCGAAAGATATCCGCGAACTGCCTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCAtgt > 2:301718/1‑149 (MQ=255)
cTCCAGCTGTAACGCCCAAACTATGGCGAAAGATATCCGCGAACTGCCTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCAtgt > 2:299496/1‑149 (MQ=255)
ccAGCTGTAACGCCCAAACTGTGGCGAAAAATATCCGCGAACTGCCTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGTCGCATGTGc < 4:106496/149‑1 (MQ=255)
cAGCTGTACCGCCCAAACTATGGCGAAAGATATCCGCGAACTGCCTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCg > 3:699320/1‑149 (MQ=255)
aGCTGTAACGCCCAAACTATGGCGAAAGATATCCGCGAACTGCCTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCgg < 1:299496/149‑1 (MQ=255)
gCTGTAACGCCCAAACTATGGCGAAAGATATCCGCGAACTGCCTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGc < 2:380358/149‑1 (MQ=255)
gTAACGCCCAAACTATGGCGAAAGATATCCGGGAACTGCCTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCAGTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCACATGCGGCttt < 1:497936/149‑2 (MQ=255)
gCCCAAACTATGGCGAAAGATATCCGCGAACTGCCTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGAtt > 2:728307/1‑149 (MQ=255)
ccAAACTATGGCGAAAGATATCCGCGAACTGCCTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGc < 3:634150/149‑1 (MQ=255)
aaaCTATGGCGAAAGATATCCGCGAACTGCCTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTg > 2:756534/1‑149 (MQ=255)
aaCTATGGCGAAAGATATCCGCGAACTGCCTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGa < 1:87388/149‑1 (MQ=255)
gAAAGATATCCGCGAACTGCCTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTgcg < 1:173414/149‑1 (MQ=255)
tatCCGCGAACTGCCTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCgttgt > 1:291802/1‑149 (MQ=255)
tatCCGCGAACTGCCTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCgttgt > 3:138055/1‑149 (MQ=255)
ccGCGAACTGCCTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTa < 1:2669/149‑1 (MQ=255)
gcgAACTGCCTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATg < 2:652955/149‑1 (MQ=255)
ccTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCa > 4:504731/1‑112 (MQ=255)
ccTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTACa < 3:504731/112‑1 (MQ=255)
gTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGt < 4:548785/122‑1 (MQ=255)
gTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGt > 3:548785/1‑122 (MQ=255)
tCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCACATGCGGCttt < 3:131607/103‑2 (MQ=255)
tCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCACATGCGGCttt > 4:131607/1‑102 (MQ=255)
cGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCgtt > 4:138683/1‑125 (MQ=255)
cGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCgtt < 3:138683/125‑1 (MQ=255)
aTTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGcc < 3:332676/149‑1 (MQ=255)
aaCGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTAt > 1:712803/1‑149 (MQ=255)
aaCGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTAt < 4:36703/149‑1 (MQ=255)
gTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGc < 2:320914/149‑1 (MQ=255)
gTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGc < 1:756534/149‑1 (MQ=255)
gTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATAAGGCGTTTACGCCGCATCCGGc < 1:298223/149‑1 (MQ=255)
tGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGcc < 1:27652/149‑1 (MQ=255)
gCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCt > 2:750104/1‑149 (MQ=255)
tttCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaaaa < 1:655919/149‑1 (MQ=255)
tCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAAtt < 4:699320/149‑1 (MQ=255)
ccGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCa > 1:543035/1‑149 (MQ=255)
cGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCaa > 3:113448/1‑149 (MQ=255)
cGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCaa < 4:425863/149‑1 (MQ=255)
cGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCaa < 1:728307/149‑1 (MQ=255)
cATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATa < 1:510665/149‑1 (MQ=255)
tGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCa > 1:707738/1‑149 (MQ=255)
tGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAg < 2:326718/149‑1 (MQ=255)
cTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCaa > 1:689110/1‑126 (MQ=255)
cTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCaa < 2:689110/126‑1 (MQ=255)
cTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGc > 1:708870/1‑149 (MQ=255)
gACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCt < 4:360613/149‑1 (MQ=255)
gACGCTGCTGGCGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCt < 1:585275/149‑1 (MQ=255)
aCGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTg > 1:595774/1‑149 (MQ=255)
gTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGGCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTa > 4:518647/1‑149 (MQ=255)
gTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTa < 4:37552/149‑1 (MQ=255)
gAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAgc < 2:708870/149‑1 (MQ=255)
gCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCa < 1:144933/149‑1 (MQ=255)
aaTAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATc < 1:478339/149‑1 (MQ=255)
aaTAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATc > 2:466101/1‑149 (MQ=255)
tAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTaaa < 1:280983/70‑1 (MQ=255)
tAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTaaa > 2:280983/1‑70 (MQ=255)
tctgaaGCCGCACATGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCATAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAgg > 3:570370/5‑149 (MQ=255)
aGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAAtt > 3:575907/1‑149 (MQ=255)
|
TACTCCAGCTGTAACGCCCAAACTATGGCGAAAGATATCCGCGAACTGCCTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATT > NC_000913/899508‑899797
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
