Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A16 F70 I0 R1	629	86.3	3068034	98.1%	3009741	142.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,248,064	T→A	5.4%	K351* (AAA→TAA)	dhaM ←	putative dihydroxyacetone‑specific PTS enzymes: HPr, EI components

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,248,064	0	T	A	5.4%	256.2 / 4.7	74	K351* (AAA→TAA)	dhaM	putative dihydroxyacetone‑specific PTS enzymes: HPr, EI components
Reads supporting (aligned to +/- strand): ref base T (33/37); new base A (2/2); total (35/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.40e-01

TAGAATAGTTGGCGAGTTAAACTGCGGGAGTTCTTCTTTCGTTTGGGTCAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCTTTC  >  NC_000913/1247918‑1248212

tAGAATAGTTGGCGAGTTAAACTGCGGGAGTTCTTCTTTCGTTTGGGTCAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTaa                                                                                                                                                    <  1:408131/149‑1 (MQ=255)

tAGAATAGTTGGCGAGTTAAACTGCGGGAGTTCTTCTTTCGTTTGGGTCAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTaa                                                                                                                                                    <  3:70945/149‑1 (MQ=255)

  gAATAGTTGGCGAGTTAAACTGCGGGAGTTCTTCTTTCGTTTGGGTCAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGa                                                                                                                                                  >  2:738258/1‑149 (MQ=255)

  gAATAGTTGGCGAGTTAAACTGCGGGAGTTCTTCTTTCGTTTGGGTCAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGa                                                                                                                                                  >  1:216754/1‑149 (MQ=255)

  gAATAGTTGGCGAGTTAAACTGCGGGAGTTCTTCTTTCGTTTGGGTCAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGa                                                                                                                                                  >  2:510481/1‑149 (MQ=255)

   aaTAGTTGGCGAGTTAAACTGCGGGAGTTCTTCTTTCGATTGGGTCAGGTGGACCAGGGTTCGATGCAGAAGATCGTCCACACCAGTATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGac                                                                                                                                                 <  2:291100/149‑2 (MQ=255)

    aTAGTTGGCGAGTTAAACTGCGGGAGTTCTTCTTTCGTTTGGGTCAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAAc                                                                                                                                                <  3:693419/149‑1 (MQ=255)

       gTTGGCGAGTTAAACTGCGGGAGTTCTTCTTTCGTTTGGGTCAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTtg                                                                                                                                             <  3:382295/149‑1 (MQ=255)

         tGGCGAGTTAAACTGCGGGAGTTCTTCTTTCGTTTGGGTCAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTtgct                                                                                                                                           <  2:620416/149‑1 (MQ=255)

           gCGAGTTAAACTGCGGGAGTTCTTCTTTCGTTTGGGTCAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTAAAGAACttagcca                                                                                                                                         <  4:225998/149‑6 (MQ=255)

             gAGTTAAACTGCGGGAGTTCTTCTTTCGTTTGGGTCAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCa                                                                                                                                       >  2:704949/1‑149 (MQ=255)

             gAGTTAAACTGCGGGAGTTCTTCTTTCGTTTGGGTCAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCa                                                                                                                                       >  2:410731/1‑149 (MQ=255)

               gTTAAACTGCGGGAGTTCTTCTTTCGTTTGGGTCAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAgg                                                                                                                                     >  1:151718/1‑149 (MQ=255)

                ttAAACTGCGGGAGTTCTTCTTTCGTTTGGGTCAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCttt                                                                                                                                                      >  4:499363/1‑131 (MQ=255)

                ttAAACTGCGGGAGTTCTTCTTTCGTTTGGGTCAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCttt                                                                                                                                                      <  3:499363/131‑1 (MQ=255)

                     cTGCGGGAGTTCTTCTTTCGTTTGGGTCAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATAtt                                                                                                                               <  4:295880/149‑1 (MQ=255)

                          ggAGTTCTTCTTTCGTTTGGGTCAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGcc                                                                                                                          <  2:151718/149‑1 (MQ=255)

                                 ttcttTCGTTTGGGTCAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAAt                                                                                                                   <  1:704949/149‑1 (MQ=255)

                                 ttcttTCGATTGGGTCAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAAt                                                                                                                   <  2:634225/149‑1 (MQ=255)

                                    tttCGTTTGGGTCAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGtt                                                                                                                >  3:129628/1‑149 (MQ=255)

                                    tttCGTTTGGGTCAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTC‑TTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTc                                                                                                               <  4:257808/149‑1 (MQ=255)

                                    tttCGTTTGGGTCAGGTGGACCACGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGtt                                                                                                                <  1:506910/149‑1 (MQ=255)

                                      tCGTTTGGGTCAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCa                                                                                                              <  2:143096/149‑1 (MQ=255)

                                      tCGTTTGGGTCAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCa                                                                                                              <  2:170278/149‑1 (MQ=255)

                                             ggTCAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGaa                                                                                                       >  3:421952/1‑149 (MQ=255)

                                              gTCAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAg                                                                                                      >  4:563243/1‑149 (MQ=255)

                                              gTCAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAg                                                                                                      <  1:738258/149‑1 (MQ=255)

                                              gTCAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTGCATGCTGAAg                                                                                                      >  3:224088/1‑149 (MQ=255)

                                                cAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGa                                                                                                    <  2:7813/149‑1 (MQ=255)

                                                          aGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGATATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTgc                                                                                          >  2:725900/1‑149 (MQ=255)

                                                           gggTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTgcc                                                                                         <  3:296791/149‑1 (MQ=255)

                                                                cGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTAAAGAACtta                                                                                                                                             >  1:287459/1‑91 (MQ=255)

                                                                cGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTAAAGAACtta                                                                                                                                             <  2:287459/92‑2 (MQ=255)

                                                                 gATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTgccgccgcc                                                                                   >  1:83614/1‑149 (MQ=255)

                                                                     cAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCcagca                                                                               >  2:66212/1‑149 (MQ=255)

                                                                       gaagaTCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGt                                                                             >  1:141099/1‑149 (MQ=255)

                                                                        aagaTCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGtt                                                                            >  4:44470/1‑149 (MQ=255)

                                                                             cGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGGTCCGGa                                                                       <  4:421952/149‑1 (MQ=255)

                                                                              gTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGat                                                                      >  1:545271/1‑149 (MQ=255)

                                                                               tCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGatc                                                                     <  1:510481/149‑1 (MQ=255)

                                                                               tCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGatc                                                                     >  3:248908/1‑149 (MQ=255)

                                                                                ccACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGatca                                                                    <  2:37506/149‑1 (MQ=255)

                                                                                  acaTCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGatcatc                                                                  <  1:712558/149‑1 (MQ=255)

                                                                                     tCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTaa                                                               <  4:248908/149‑1 (MQ=255)

                                                                                         tataGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAgt                                                           <  4:224088/149‑1 (MQ=255)

                                                                                           taGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAgtgt                                                         <  1:13550/149‑1 (MQ=255)

                                                                                           taGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCCTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAgtgt                                                         >  4:47929/1‑149 (MQ=255)

                                                                                            aGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTa                                                        <  3:180104/149‑1 (MQ=255)

                                                                                            aGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTa                                                        <  2:580223/149‑1 (MQ=255)

                                                                                               gAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATgg                                                     >  4:407316/1‑149 (MQ=255)

                                                                                               gAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATACTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATgg                                                     >  4:564143/1‑149 (MQ=255)

                                                                                                aGCTTGTAGATATTCATCATCCAGTTGCTGATATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGt                                                    <  1:725900/149‑1 (MQ=255)

                                                                                                   ttGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGAc                                                 <  3:411962/149‑1 (MQ=255)

                                                                                                        gATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGaa                                            >  2:420266/1‑149 (MQ=255)

                                                                                                            ttCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACa                                                             <  3:406594/128‑1 (MQ=255)

                                                                                                            ttCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACa                                                             >  4:406594/1‑128 (MQ=255)

                                                                                                                tcatcCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGc                                    >  4:551909/1‑149 (MQ=255)

                                                                                                                tcatcCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGc                                    >  1:234300/1‑149 (MQ=255)

                                                                                                                tcatcCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGc                                    <  3:551909/149‑1 (MQ=255)

                                                                                                                 catcCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCg                                   <  2:141099/149‑1 (MQ=255)

                                                                                                                 catcCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCg                                   <  1:53569/149‑1 (MQ=255)

                                                                                                                    ccAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGAt                                       >  3:728162/1‑142 (MQ=255)

                                                                                                                    ccAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGAt                                       <  4:728162/142‑1 (MQ=255)

                                                                                                                     cAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGCaa                               <  2:116349/149‑1 (MQ=255)

                                                                                                                               tATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAg                     >  4:735623/1‑149 (MQ=255)

                                                                                                                                     tGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCt               >  2:763720/1‑149 (MQ=255)

                                                                                                                                      ggCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTg              >  1:662192/1‑149 (MQ=255)

                                                                                                                                         tAAGTTCTTTAGGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCAt                                                                                                             >  3:490132/1‑51 (MQ=255)

                                                                                                                                         tAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCAt                                                                                                             <  4:490132/51‑1 (MQ=255)

                                                                                                                                         tAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGc            <  4:263303/148‑1 (MQ=255)

                                                                                                                                         tAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGc            >  3:263303/1‑148 (MQ=255)

                                                                                                                                            gTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCt        >  1:74139/1‑149 (MQ=255)

                                                                                                                                            gTTCTTAAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCt        >  2:601791/1‑149 (MQ=255)

                                                                                                                                             ttCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAATATc                           <  2:372604/129‑1 (MQ=255)

                                                                                                                                             ttCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAATATc                           >  1:372604/1‑129 (MQ=255)

                                                                                                                                             ttCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTg       >  3:71395/1‑149 (MQ=255)

                                                                                                                                                tttAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCtt    >  1:111658/1‑149 (MQ=255)

                                                                                                                                                tttAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCtt    >  3:191022/1‑149 (MQ=255)

                                                                                                                                                 ttAAGACCTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGTGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCttt   <  3:564143/149‑1 (MQ=255)

                                                                                                                                                  tAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCTTTc  <  3:564110/149‑1 (MQ=255)

                                                                                                                                                  tAAGAACTTGCTGCCAGGCATATTCTGACGTGCAATGTTCATGCTGAAGAAGTTCGCTTGCCGCCGCCAGCACTTCCGGATCATCTAACAGTGTCTGGTGACCAGAAAAGATTGCGGCAAGATCGTCAAGCCCGCTGGCTTCTGCTTTc  <  2:692323/149‑1 (MQ=255)

TAGAATAGTTGGCGAGTTAAACTGCGGGAGTTCTTCTTTCGTTTGGGTCAGGTGGACCAGGGTGCGATGCAGAAGATCGTCCACATCAATATAGCGAGCTTGTAGATATTCATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAATGTTCATGCTGAAGGAGTTCGCTTGCCGCCGCCAGCAGTTCCGGATCATCTAACAGTGTATGGTGACCAGAAAAGATTGCGGCAATATCGTCAAGCCCGCTGGCTTCTGCTTTC  >  NC_000913/1247918‑1248212

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A