Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A16 F70 I0 R1 629 86.3 3068034 98.1% 3009741 142.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,367,637 T→G 5.5% N189K (AAT→AAG)  arnC → undecaprenyl phosphate‑L‑Ara4FN transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,367,6370TG 5.5% 252.5 / 6.4 74N189K (AAT→AAG) arnCundecaprenyl phosphate‑L‑Ara4FN transferase
Reads supporting (aligned to +/- strand):  ref base T (28/41);  new base G (2/2);  total (30/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.79e-01

TCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGAACGCAGCACCTTTATCCCGATTCTGGCGAATATCTTCGCCCGCCGTGCCATTGAAATTCCAGTACATCATGCCGAGCGTGAGTTTGGTGAATCCAAATACAGTTTTATGCGCCTGATTAATTTGATGTACGACCTGGTGACCTGCCTTACCACAACGCCGCTACGTATGCTGAGTCTGC  >  NC_000913/2367491‑2367785
                                                                                                                                                  |                                                                                                                                                    
tCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGAACGCAGCACCTTTATCCCGATTCTGGCGAatat                                                                                                                                                    <  2:26364/149‑1 (MQ=255)
  gAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGAACGCAGCACCTTTATCCCGATTCTGGCGAATATCt                                                                                                                                                  >  3:324824/1‑149 (MQ=255)
    aGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGAACGCAGCACCTTTATCCCGATTCTGGCGAATATCTTc                                                                                                                                                <  2:633463/149‑1 (MQ=255)
    aGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGAACGCAGCACCTTTATCCCGATTCTGGCGAATATCTTc                                                                                                                                                <  3:193024/149‑1 (MQ=255)
        gatTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGAACGCAGCACCTTTATCCCGATTCTGGCGAATATCTTcgccc                                                                                                                                            <  1:484109/149‑1 (MQ=255)
         atTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGAACGCAGCACCTTTATCCCGATTCTGGCGAAGATATTcgccag                                                                                                                                           <  4:312323/149‑3 (MQ=255)
         atTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGAACGCAGCACCTTTATCCCGATTCTGGCGAAGATATTcgccag                                                                                                                                           <  4:325324/149‑3 (MQ=255)
         atTAACCGGCTTATTCAGCGCAACACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGAACGCAGCACCTTTATCCCGATTCTGGCGAATATCTTcgcccg                                                                                                                                           >  2:9256/1‑149 (MQ=255)
              ccGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGAACGCAGCACCTTTATCCCGATTCTGGCGAATATCTTCGCCCGCCGTg                                                                                                                                      >  1:60860/1‑149 (MQ=255)
                ggCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGAACGCAGCACCTTTATCCCGATTCTGGCGAATATCTTCGCCCGCCGTGcc                                                                                                                                    >  1:533606/1‑149 (MQ=255)
                           cgcACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGAACGCAGCACCTTTATCCCGATTCTGGCGAATATCTTCGCCCGCCGTGCCATTGAAATTcc                                                                                                                         <  1:209506/149‑1 (MQ=255)
                             caccacTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGAACGCAGCACCTTTATCCCGATTCTGGCGAATATCTTCGCCCGCCGTGCCATTGAAATTCCAg                                                                                                                       <  1:240312/149‑1 (MQ=255)
                               ccacTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGAACGCAGCACCTTTATCCCGATTCTGGCGAATATCTTCGCCCGCCGTGCCATTGAAATTCCAGTa                                                                                                                     >  3:277113/1‑149 (MQ=255)
                                   tGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGAACGCAGCACCTTTATCCCGATTCTGGCGAATATCTTCGCCCGCCGTGCCATTGAAATTCCAGTAcatc                                                                                                                 >  3:185529/1‑149 (MQ=255)
                                    ggCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGAACGCAGCACCTTTATCCCGATTCTGGCGAATATCTTCGCCCGCCGTGCCATTGAAATTCCAGTAcatca                                                                                                                <  4:217032/149‑1 (MQ=255)
                                      cAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGAACGCAGCACCTTTATCCCGATTCTGGCGAATATCTTCGCCCGCCGTGCCATTGAAATTCCAGTACATCATg                                                                                                              <  4:498232/149‑1 (MQ=255)
                                       aaaGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGAACGCAGCACCTTTATCCCGATTCTGGCGAATATCTTCGCCCGCCGTGCCATTGAAATTCCAGTACATCATGc                                                                                                             >  1:32690/1‑149 (MQ=255)
                                        aaGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGAACGCAGCACCTTTATCCCGATTCTGGCGAATATCTTCGCCCGCCGTGCCATTGAAATTCCAGTACATCATGcc                                                                                                            >  2:767694/1‑149 (MQ=255)
                                              tGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGAACGCAGCACCTTTATCCCGATTCTGGCGAATATCTTCGCCCGCCGTGCCATTGAAATTCCAGTACATCATGCCGAGCGt                                                                                                      <  4:196113/149‑1 (MQ=255)
                                                ggTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGAACGCAGCACCTTTATCCCGATTCTGGCGAATATCTTCGCCCGCCGTGCCATTGAAATTCCAGTACATCATGCCGAGCGTGa                                                                                                    <  3:700381/149‑1 (MQ=255)
                                                 gTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGAACGCAGCACCTTTATCCCGATTCTGGCGAATATCTTCGCCCGCCGTGCCATTGAAATTCCAGTACATCATGCCGAGCGTGAg                                                                                                   <  2:533606/149‑1 (MQ=255)
                                                  tGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGAACGCAGCACCTTTATCCCGATTCTGGCGAATATCTTCGCCCGCCGTGCCATTGAAATTCCAGTACATCATGCCGAGCGTGAGt                                                                                                  >  4:487469/1‑149 (MQ=255)
                                                  tGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGAACGCAGCACCTTTATCCCGATTCTGGCGAATATCTTCGCCCGCCGTGCCATTGAAATTCCAGTACATCATGCCGAGCGTGAGt                                                                                                  >  3:275088/1‑149 (MQ=255)
                                                    aTTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGAACGCAGCACCTTTATCCCGATTCTGGCGAATATCTTCGCCCGCCGTGCCATTGAAATTCCAGTACATCATGCCGAGCGTGAGttt                                                                                                <  1:362918/149‑1 (MQ=255)
                                                     ttACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGAACGCAGCACCTTTATCCCGATTCTGGCGAATATCTTCGCCCGCCGTGCCATTGAAATTCCAGTACATCATGCCGAGCGTGAGTTTg                                                                                               <  2:549212/149‑1 (MQ=255)
                                                      tACGGTTGTATGCTGCGCGCCTATCGCCGTCATCTTGTCGATGCGATGTTGCACTGCCATGAACGCAGCACCTTTATCCCGATTCTGGCGAATATCTTCGCCCGCCGTGCCATTGAAATTCCAGTACATCATGCCGAGCGTGAGTTTgg                                                                                              <  4:500615/149‑1 (MQ=255)
                                                       aCGTTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGAACGCAGCACCTTTTTCCCGATTCTGGCGAAAATCTTCGCCCGCCGTGCCATTGAAATTCCAGTACATCATGCCGAGCGTGAGTTTGGt                                                                                             <  2:694533/149‑1 (MQ=255)
                                                       aCGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGAACGCAGCACCTTTATCCCGATTCTGGCGAATATCTTCGCCCGCCGTGCCATTGAAATTCCAGTACATCATGCCGAGCGTGAGTTTGGt                                                                                             <  3:487469/149‑1 (MQ=255)
                                                       aCGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGAACGCAGCACCTTTATCCCGATTCTGGCGAATATCTTCGCCCGCCGTGCCATTGAAATTCCAGTACATCATGCCGAGCGTGAGTTTGGt                                                                                             <  2:536829/149‑1 (MQ=255)
                                                            tGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGAACGCAGCACCTTTATCCCGATTCTGGCGAATATCTTCGCCCGCCGTGCCATTGAAATTCCAGTACATCATGCCGAGCGTGAGTTTGGTGAATc                                                                                        <  4:324824/149‑1 (MQ=255)
                                                              tATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGAACGCAGCACCTTTATCCCGATTCTGGCGAATATCTTCGCCCGCCGTGCCATTGAAATTCCAGTACATCATGCCGAGCGTGAGTTTGGTGAATCCa                                                                                      >  1:481330/1‑149 (MQ=255)
                                                                          cTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGAACGCAGCACCTTTATCCCGATTCTGGCGAATATCTTCGCCCGCCGTGCCATTGAAATTCCAGTACATCATGCCGAGCGTGAGTTTGGTGAATCCAAATACAGTTTTa                                                                          >  2:767876/1‑149 (MQ=255)
                                                                          cTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGAACGCAGCACCTTTATCCCGATTCTGGCGAATATCTTCGCCCGCCGTGCCATTGAAATTCCAGTACATCATGCCGAGCGTGAGTTTGGTGAATCCAAATACAGTTTTa                                                                          >  2:246002/1‑149 (MQ=255)
                                                                               gCCGTCATATTGTCGATGCGATGTTGCACTGCCATGAACGCAGCACCTTTATCCCGATTCTGGCGAATATCTTCGCCCGCCGTGCCATTGAAATTCCAGTACATCATGCCGAGCGTGAGTTTGGTGAATCCAAATACAGTTTTATgcgc                                                                     >  1:294242/1‑149 (MQ=255)
                                                                                 cGTCATATTGTCGATGCGATGTTGCACTGCCATGAACGCAGCACCTTTATCCCGATTCTGGCGAATATCTTCGCCCGCCGTGCCATTGAAATTCCAGTACATCATGCCGAGCGTGAGTTTGGTGAATCCAAATACAGTTTTATGCGCCt                                                                   <  1:717213/149‑1 (MQ=255)
                                                                                        ttGTCGATGCGATGTTGCACTGCCATGAACGCAGCACCTTTATCCCGATTCTGGCGAATATCTTCGCCCGCCGTGCCATTGAAATTCCAGTACATCATGCCGAGCGTGAGTTTGGTGAATCCAAATACAGTTTTATGCGCCt                                                                   <  2:75871/142‑1 (MQ=255)
                                                                                        ttGTCGATGCGATGTTGCACTGCCATGAACGCAGCACCTTTATCCCGATTCTGGCGAATATCTTCGCCCGCCGTGCCATTGAAATTCCAGTACATCATGCCGAGCGTGAGTTTGGTGAATCCAAATACAGTTTTATGCGCCt                                                                   >  1:75871/1‑142 (MQ=255)
                                                                                        ttGTCGATGCGATGTTGCACTGCCATGAACGCAGCACCTTTATCCCGATTCTGGCGAATATCTTCGCCCGCCGTGCCATTGAAATTCCAGTACATCATGCCGAGCGTGAGTTTGGTGAATCCAAATACAGTTTTATGCGCCTGATTAAt                                                            <  2:60860/149‑1 (MQ=255)
                                                                                          gTCGATGCGATGTTGCACTGCCATGAACGCAGCACCTTTATCCCGATTCTGGCGAATATCTTCGCCCGCCGTGCCATTGAAATTCCAGTACATCATGCCGAGCGTGAGTTTGGTGAATCCAAATACAGTTTTATGCGCCTGATTAAttt                                                          <  1:92269/149‑1 (MQ=255)
                                                                                            cgatgcgatgTTGCACTGCCATGAACGCAGCACCTTTATCCCGATTCTGGCGAATATCTTCGCCCGCCGTGCCATTGAAATTCCAGTACATCATGCCGAGCGTGAGTTTGGTGAATCCAAATACAGTTTTATGCGCCTGATTAATTTGa                                                        <  2:483558/149‑1 (MQ=255)
                                                                                             gatgcgatgTTGCACTGCCATGAACGCAGCACCTTTATCCCGATTCTGGCGAATATCTTCGCCCGCCGTGCCATTGAAATTCCAGTACATCATGCCGAGCGTGAGTTTgg                                                                                              >  3:586356/1‑110 (MQ=255)
                                                                                             gatgcgatgTTGCACTGCCATGAACGCAGCACCTTTATCCCGATTCTGGCGAATATCTTCGCCCGCCGTGCCATTGAAATTCCAGTACATCATGCCGAGCGTGAGTTTgg                                                                                              <  4:586356/110‑1 (MQ=255)
                                                                                             gatgcgatgTTGCACTGCCATGAACGCAGCACCTTTATCCCGATTCTGGCGAATATCTTCGCCCGCCGTGCCATTGAAATTCCAGTACATCATGCCGAGCGTGAGTTTGGTGAATCCAAATACAGTTTTATGCGCCTGATTAATTTGAt                                                       <  2:577534/149‑1 (MQ=255)
                                                                                               tgcgatgTTGCACTGCCATGAACGCAGCACCTTTATCCCGATTCTGGCGAATATCTTCGCCCGCCGTGCCATTGAAATTCCAGTACATCATGCCGAGCGTGAGTTTGGTGAATCCAAATACAGTTTTATGCGCCTGATTAAt                                                            >  3:547538/1‑142 (MQ=255)
                                                                                               tgcgatgTTGCACTGCCATGAACGCAGCACCTTTATCCCGATTCTGGCGAATATCTTCGCCCGCCGTGCCATTGAAATTCCAGTACATCATGCCGAGCGTGAGTTTGGTGAATCCAAATACAGTTTTATGCGCCTGATTAAt                                                            <  4:547538/142‑1 (MQ=255)
                                                                                                 cgatgTTGCACTGCCATGAACGCAGCACCTTTATCCCGATTCTGGCGAATATCTACGCCCGCCGTGCCATTGAAATTCCAGTACATCATGCCGAGCGTGAGTTTGGTGAATCCAAATACAGTTTTATGCGCCTGATTAATTTGATGTAc                                                   <  1:134348/149‑1 (MQ=255)
                                                                                                     gTTGCACTGCCATGAACGCAGCACCTTTATCCCGATTCTGGCGAATATCTTCGCCCGCCGTGCCATTGAAATTCCAGTACATCATGCCGAGCGTGAGTTTGGTGAATCCAAATACAGTTTTATGCGCCTGATTAATTTGATGTACGAcc                                               >  1:66452/1‑149 (MQ=255)
                                                                                                       tGCACTGCCATGAACGCAGCACCTTTATCCCGATTCTGGCGAATATCTTCGCCCGCCGTGCCATTGAAATTCCAGTACATCATGCCGAGCGTGAGTTTGGTGAATCCAAATACAGTTTTATGCGCCTGATTAATTTGATGTACGACCTg                                             <  2:76782/149‑1 (MQ=255)
                                                                                                         cACTGCCATGAACGCAGCACCTTTATCCCGATTCTGGCGAATATCTTCGCCCGCCGTGCCATTGAAATTCCAGTACATCATGCCGAGCGTGAGTTTGGTGAATCCAAATACAGTTTTATGCGCCTGATTAATTTGATGTACGACCTGGt                                           >  4:67640/1‑149 (MQ=255)
                                                                                                          aCTGCCATGAACGCAGCACCTTTATCCCGATTCTGGCGAATATCTTCGCCCGCCGTGCCATTGAAATTCCAGTACATCATGCCGAGCGTGAGTTTGGTGAATCCAAATACAGTTTTATGCGCCTGATTAATTTGATGTACGACCTGGTg                                          <  3:152348/149‑1 (MQ=255)
                                                                                                              ccATGAACGCAGCACCTTTATCCCGATTCTGGCGAATATCTTCGCCCGCCGTGCCATTGAAATTCCAGTACATCATTCCGAGCGTGAGTTTGGTGAATCCAAATACAGTTTTATGCGCCTGATTAATTTGATGTACGACCTGGTGACCt                                      <  3:482540/149‑1 (MQ=255)
                                                                                                              ccATGAACGCAGCACCTTTATCCCGATTCTGGCGAATATCTTCGCCCGCCGTGCCATTGAAATTCCAGTACATCATGCCGAGCGTGAGTTTGGTGAATCCAAATACAGTTTTATGCGCCTGATTAATTTGATGTACGACCTGGTGACCt                                      <  4:79240/149‑1 (MQ=255)
                                                                                                              ccATGAACGCAGCACCTTTATCCCGATTCTGGCGAATATCTTCGCCCGCCGTGCCATTGAAATTCCAGTACATCATGCCGAGCGTGAGTTTGGTGAATCCAAATACAGTTTTATGCGCCTGATTAATTTGATGTACGACCTGGTGACCt                                      >  2:115951/1‑149 (MQ=255)
                                                                                                                aTGAACGCAGCACCTTTATCCCGATTCTGGCGAATATCTTCGCCCGCCGTGCCATTGAAATTCCAGTACATCATGCCGAGCGTGAGTTTGGTGAATCCAAATACAGTTTTATGCGCCTGATTAATTTGATGTACGACCTGGTGACCTGc                                    >  4:451883/1‑149 (MQ=255)
                                                                                                                    aCGCAGCACCTTTATCCCGATTCTGGCGAATATCTTCGCCCGCCGTGCCATTGAAATTCCAGTACATCATGCCGAGCGTGAGTTTGGTGAATCCAAATACAGTTTTATGCGCCTGATTAATTTGATGTACGACCTGGTGACCTGCCTTa                                <  4:277113/149‑1 (MQ=255)
                                                                                                                     cGCAGCACTTTTAGCCCGATTCTGGCGAATATCTTCGCCCGCCGTGCCATTGAAATTCCAGTACATCATGCCGAGCGTGAGTTTGGTGAATCCAAATACAGTTTTATGCGCCTGAGTAATTTGATGTACGACCTGGTGACCTGCCTTAc                               <  4:514061/149‑1 (MQ=255)
                                                                                                                          caCCTTTATCCCGATTCTGGCGAATATCTTCGCCCGCCGTGCCATTGAAATTCCAGTACATCATGCCGAGCGTGAGTTTGGTGAATCCAAATACAGTTTTATGCGCCTGATTAATTTGATGTACGACCTGGTGACCTGCCTTACCACaa                          >  4:192502/1‑149 (MQ=255)
                                                                                                                          caCCTTTATCCCGATTCTGGCGAATATCTTCGCCCGCCGTGCCATTGAAATTCCAGTACATCATGCCGAGCGTGAGTTTGGTGAATCCAAATACAGTTTTATGCGCCTGATTAATTTGATGTACGACCTGGTGACCTGCCTTACCACaa                          >  3:516583/1‑149 (MQ=255)
                                                                                                                           aCCTTTATCCCGATTCTGGCGAATATCTTCGCCCGCCGTGACATTGAAATTCCAGTACATCATGCCGAGCGTGAGTTTGGTGAATCCAAATACAGTTTTATGCGCCTGATTAATTTGATGTACGACCTGGTGACCTGCCTTACCACAAc                         <  2:77856/149‑1 (MQ=255)
                                                                                                                              tttATCCCGATTCTGGCGAATATCTTCGCCCGCCGTGCCATTGAAATTCCAGTACATCATGCCGAGCGTGAGTTTGGTGAATCCAAATACAGTTTTATGCGCCTGATTAATTTGATGTACGACCTGGTGACCTGCCTTACCACAAcgcc                      >  4:109336/1‑149 (MQ=255)
                                                                                                                              tttATCCCGATTCTGGCGAATATCTTCGCCCGCCGTGCCATTGAAATTCCAGTACATCATGCCGAGCGTGAGTTTGGTGAATCCAAATACAGTTTTATGCGCCTGATTAATTTGATGTACGACCTGGTGACCTGCCTTACCACAAcgcc                      >  1:372633/1‑149 (MQ=255)
                                                                                                                                  tCCCGATTCTGGCGAATATCTTCGCCCGCCGTGCCATTGAAATTCCAGTACATCATGCCGAGCGTGAGTTTGGTGAATCCAAATACAGTTTTATGCGCCTGATTAATTTGATGTACGACCTGGTGACCTGCCTTACCACAACGCCGCTa                  >  1:454922/1‑149 (MQ=255)
                                                                                                                                   cccGATTCTGGCGAATATCTTCGCCCGCCGTGCCATTGAAATTCCAGTACATCATGCCGAGCGTGAGTTTGGTGAATCCAAATACAGTTTTATGCGCCTGATTAATTTGATGTACGACCTGGTGACCTGCCTTACCACAACGCCGCTAc                 <  3:67640/149‑1 (MQ=255)
                                                                                                                                    ccGATTCTGGCGAATATCTTCGCCCGCCGTGCCATTGAAATTCCAGTACATCATGCCGAGCGTGAGTTTGGTGAATCCAAATACAGTTTTATGCGCCTGATTAATTTGATGTACGACCTGGTGACCTGCCTTACCACAACGCCGCTACg                >  2:298237/1‑149 (MQ=255)
                                                                                                                                     cGATTCTGGCGAATATCTTCGCCCGCCGTGCCATTGAAATTCCAGTACATCATGCCGAGCGTGAGTTTGGTGAATCCAAATACAGTTTTATGCGCCTGATTAATTTGATGTACGACCTGGTGACCTGc                                    <  3:437186/128‑1 (MQ=255)
                                                                                                                                     cGATTCTGGCGAATATCTTCGCCCGCCGTGCCATTGAAATTCCAGTACATCATGCCGAGCGTGAGTTTGGTGAATCCAAATACAGTTTTATGCGCCTGATTAATTTGATGTACGACCTGGTGACCTGc                                    >  4:437186/1‑128 (MQ=255)
                                                                                                                                        ttCTGGCGAATATCTTCGCCCGCCGTGCCATTTAAATTCCAGTACATCATGCTGAGCGTGAGTTTGGTGAATCCAAATACAGTTTTATGCGCCTAATTAATTTGATGTACGACCTGGTGACCTGCCTTACCACAACGCCGCTACGTATg            <  1:456479/149‑1 (MQ=255)
                                                                                                                                        ggcgggCGAAGATATTCGCCCGCCGTGCCATTGAAATTCCAGTACATCATGCCGAGCGTGAGTTTGGTGAATCCAAATACAGTTTTATGCGCCTGATTAATTTGATGTACGACCTGGTGACCTGCCTTACCACAACGCCGCTACGTATg            >  3:294842/5‑149 (MQ=255)
                                                                                                                                         tCTGGCGAATATCTTCGCCCGCCGTGCCATTGAAATTCCAGTACATCATGCCGAGCGTGAGTTTGGTGAATCCAAATACAGTTTTATGCGCCTGATTAATTTGATGTACGACCTGGTGACCTGCCTTACCACAACGCCGCTACGTATGc           <  2:696760/149‑1 (MQ=255)
                                                                                                                                         tCTGGCGAATATCTTCGCCCGCCGTGCCATTGAAATTCCAGTACATCATGCCGAGCGTGAGTTTGGTGAATCCAAATACAGTTTTATGCGCCTGATTAATTTGATGTACGACCTGGTGACCTGCCTTACCACAACGCCGCTACGTATGc           <  4:516583/149‑1 (MQ=255)
                                                                                                                                         tCTGGCGAATATCTTCGCCCGCCGTGCCATTGAAATTCCAGTACATCATGCCGAGCGTGAGTTTGGTGAATCCAAATACAGTTTTATGCGCCTGATTAATTTGATGTACGACCTGGTGACCTGCCTTACCACAACGCCGCTACGTATGc           <  3:192502/149‑1 (MQ=255)
                                                                                                                                         tCTGGCGAATATCTTCGCCCGCCGTGCCATTGAAATTCCAGTACATCATGCCGAGCGTGAGTTTGGTGAATCCAAATACAGTTTTATGCGCCTGATTAATTTGATGTACGACCTGGTGACCTGCCTTACCACAACGCCGCTACGTATGc           <  1:115951/149‑1 (MQ=255)
                                                                                                                                          cTGGCGAATATCTTCGCCCGCCGTGCCATTGAAATTCCAGTACATCATGCCGAGCGTGAGTTTGGTGAATCCAAATACAGTTTTATGCGCCTGATTAATTTGATGTACGACCTGGTGACCTGCCTTACCACAACGCCGCTACGTATGCt          <  3:707726/149‑1 (MQ=255)
                                                                                                                                           gggCGAAGATATTCGCCCGCCGTGCCATTGAAATTCCAGTACATCATGCCGAGCGTGAGTTTGGTGAATCCAAATACAGTTTTATGCGCCTGATTAATTTGATGTACGACCTGGTGACCTGCCTTACCACAACGCCGCTACGTATGCTg         >  4:507091/2‑149 (MQ=255)
                                                                                                                                              cGAATATCTTCGCCCGCCGTGCCATTGAAATTCCAGTACATCATGCCGAGCGTGAGTTTGGTGAATCCAAATACAGTTTTATGCGCCTGATTAATTTGATGTACGACCTGGTGACCTGCCTTACCACAACGCCGCTACGTATGCTGAGt      <  4:712582/149‑1 (MQ=255)
                                                                                                                                                  tatCTTCGCCCGCCGTGCCATTGAAATTCCAGTACATCATGCCGAGCGTGAGTTTGGTGAATCCAAATACAGTTTTATGCGCCTGATTAATTTGATGTACGACCTGGTGACCTGCCTTACCACAACGCCGCTACGTATGCTGAGTCTGc  >  3:378729/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
TCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGAACGCAGCACCTTTATCCCGATTCTGGCGAATATCTTCGCCCGCCGTGCCATTGAAATTCCAGTACATCATGCCGAGCGTGAGTTTGGTGAATCCAAATACAGTTTTATGCGCCTGATTAATTTGATGTACGACCTGGTGACCTGCCTTACCACAACGCCGCTACGTATGCTGAGTCTGC  >  NC_000913/2367491‑2367785

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A