Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A13 F69 I0 R1
|
1426 |
148.0 |
5875178 |
97.4% |
5722423 |
123.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
897,968:1 |
+A |
19.4% |
intergenic (+39/‑21) |
potI → / → ybjO |
putrescine ABC transporter permease/DUF2593 family inner membrane protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 897,968 | 1 | . | A | 19.4%
| 86.7
/ 26.3
| 31 | intergenic (+39/‑21) | potI/ybjO | putrescine ABC transporter permease/DUF2593 family inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base . (12/13); new base A (3/3); total (15/16) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.12e-01 |
AACATTGATCCTCGGCGCGGTCGGAATTGTCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGCCTCGACGTGC > NC_000913/897821‑898109
|
aaCATTGATCCTCGGCGCGGTCGGAATTGTCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCgcg‑c < 3:423686/149‑1 (MQ=255)
cATTGATCCTCGGCGCGGTCGGAATTGTCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CAt < 4:1041132/149‑1 (MQ=255)
aTTGATCCTCGGCGCGGTCGGAATTGTCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCcgcgacat < 1:1117698/149‑5 (MQ=255)
aTTGATCCTCGGCGCGGTCGGAATTGTCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATg < 2:460969/149‑1 (MQ=255)
gcgGTCGGAATTGTCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGACATG‑CGCGGCAttat > 1:79771/1‑146 (MQ=255)
gcgGTCGGAATTGTCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCGACATG‑CGCGGCAttat < 2:79771/148‑3 (MQ=255)
tCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCa < 1:706390/131‑1 (MQ=255)
tCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCa > 2:706390/1‑131 (MQ=255)
tATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTg > 3:378585/1‑140 (MQ=255)
tATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTg < 4:378585/140‑1 (MQ=255)
tCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTaa < 4:946258/149‑1 (MQ=255)
tCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTaa > 3:946258/1‑149 (MQ=255)
gcgcAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCa > 3:934019/1‑110 (MQ=255)
gcgcAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCa < 4:934019/110‑1 (MQ=255)
gAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTaa < 3:430442/133‑1 (MQ=255)
gAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTaa > 4:430442/1‑133 (MQ=255)
gAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACAt < 4:314807/141‑1 (MQ=255)
gAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACAt > 3:314807/1‑141 (MQ=255)
aaaaaCAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACACAATGCCGCGACATG‑CGCGGCAttat > 4:391080/1‑97 (MQ=38)
aaaaaCAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACACAATGCCGCGACATG‑CGCGGCAttat < 3:391080/99‑3 (MQ=38)
gcgACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGa < 2:138024/96‑1 (MQ=255)
gcgACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGa > 1:138024/1‑96 (MQ=255)
aGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGaa < 4:308450/138‑1 (MQ=255)
aGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGaa > 3:308450/1‑138 (MQ=255)
ggCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGtt < 3:1238590/82‑1 (MQ=255)
ggCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGtt > 4:1238590/1‑82 (MQ=255)
gACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCt < 1:616646/124‑1 (MQ=255)
gACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCt > 2:616646/1‑124 (MQ=255)
aaaTTTGCGAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCattat > 3:1348151/1‑149 (MQ=255)
caaTGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTgg < 2:557715/102‑1 (MQ=255)
caaTGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTgg > 1:557715/2‑103 (MQ=255)
aTGCCGCGACATG‑CGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAg > 2:35282/1‑106 (MQ=255)
aTGCCGCGACATG‑CGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAg < 1:35282/106‑1 (MQ=255)
cgcg‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGCCTCGACGTGc < 2:123213/145‑1 (MQ=255)
cgcg‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGCCTCGACGTGc > 1:123213/1‑145 (MQ=255)
|
AACATTGATCCTCGGCGCGGTCGGAATTGTCGGATTTATCGCCTGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGTGGCTGAAGACACTAAAATTTGCCAACCTGGCTACATAATGCCGCG‑CATGTCGCGGCATTGTTTTCATGGAAGACGAAACGTTGGGATTTTTTAAGAAAACATCTTCATCTCATGCTCGCCTGAATGTGCCTGCGCTGGTGCAGGTGGCGGCGCTCGCCATTATTATGATCCGTGGCCTCGACGTGC > NC_000913/897821‑898109
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
