Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate | Predicted Mutations | Mean Coverage | Total Reads | Percent Mapped | Mapped Reads | Average Read Length |
|---|---|---|---|---|---|---|
| A13 F69 I0 R1 | 1426 | 148.0 | 5875178 | 97.4% | 5722423 | 123.7 |
Breseq alignment
| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,449,050 | T→A | 35.2% | intergenic (+32/+26) | feaB → / ← tynA | phenylacetaldehyde dehydrogenase/tyramine oxidase, copper‑requiring |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,449,050 | 0 | T | A | 35.2% | 35.1 / 40.9 | 37 | intergenic (+32/+26) | feaB/tynA | phenylacetaldehyde dehydrogenase/tyramine oxidase, copper‑requiring |
| Reads supporting (aligned to +/- strand): ref base T (13/11); new base A (6/7); total (19/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.37e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.35e-02 | |||||||||||
GCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGT‑AAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGGCGCGGGCCACATGTG > NC_000913/1448911‑1449197 | gCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGT‑AAAAAACGGCACCAGGTGCCGtt > 4:1425070/1‑149 (MQ=255)gCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTGCGCTCATAAGT‑AAAAAACGGCACCTg > 3:881632/1‑141 (MQ=255)gCTAACTTACCGTTTGGTGGGATGAAGCAGTCACGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGAGGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGT‑AAAAAACGGCACCTg < 4:881632/141‑1 (MQ=255) cTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGT‑AAAAAACGGCACCAGGTGCCGttt < 3:1425070/149‑1 (MQ=255) tAACTTACCGTTTGGTGGGATGAAGCAGTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGT‑AAAAAACGGCACCAGGTGCCGtttt < 1:424599/149‑1 (MQ=255) ggATGAAGCAGTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAAACGGCACCAGGTGCCGttt < 3:270462/132‑1 (MQ=255) ggATGAAGCAGTCAGGAACAGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAAACGGCACCAGGTGCCGttt > 4:270462/1‑132 (MQ=255) tGAAGCAGTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGT‑AAAAAACGGCACCTGGTGCCGttt < 1:1342375/128‑1 (MQ=255) tGAAGCAGTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGGGTGTACGGTATTAATCTGGTTCGCTCATAAGT‑AAAAAACGGCACCTGGCGCCGttt > 2:1342375/1‑128 (MQ=255) aGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGT‑AAAAAACGGCACCTGGTGCCGtt < 4:558540/117‑1 (MQ=255) aGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGT‑AAAAAACGGCACCTGGTGCCGtt > 3:558540/1‑117 (MQ=255) aGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGT‑AAAAAACGGCACCAGGTGCCGttt > 4:377657/1‑118 (MQ=255) aGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGT‑AAAAAACGGCACCAGGTGCCGttt < 3:377657/118‑1 (MQ=255) gggCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGT‑AAAAAACGGCACCTGGTGCCGttttttt > 3:789557/1‑116 (MQ=255) gggCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGT‑AAAAAACGGCACCTGGTGCCGttttttt < 4:789557/116‑1 (MQ=255) cccGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGT‑AAAAAACGGCACCTGGTGCCGttt > 2:388782/1‑96 (MQ=255) cccGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGT‑AAAAAACGGCACCTGGTGCCGttt < 1:388782/96‑1 (MQ=255) cccGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGT‑AAAAAACGGCACCTGGTGCCGttt > 2:625268/1‑96 (MQ=255) cccGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGT‑AAAAAACGGCACCTGGTGCCGttt < 1:625268/96‑1 (MQ=255) tggACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGT‑AAAAAACGGCACCAGGTGCCGtttt > 1:532180/1‑87 (MQ=255) tggACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGT‑AAAAAACGGCACCAGGTGCCGtttt < 2:532180/87‑1 (MQ=255) gACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGT‑AAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGaaa < 3:452614/96‑1 (MQ=255) gACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGT‑AAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGaaa > 4:452614/1‑96 (MQ=255) tgAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGT‑AAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCa < 3:773668/105‑1 (MQ=255) tgAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGT‑AAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCa > 4:773668/1‑105 (MQ=255) tAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGT‑AAAAAACGGCACCAGGTGCCGttt < 1:240679/67‑1 (MQ=255) tAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGT‑AAAAAACGGCACCAGGTGCCGttt > 2:240679/1‑67 (MQ=255) tATTAATCTGGTTCGCTCATAAGT‑AAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTAtct > 4:104457/1‑73 (MQ=255) tATTAATCTGGTTCGCTCATAAGT‑AAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTAtct < 3:104457/73‑1 (MQ=255) cTCATAAGT‑AAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCAct < 3:227764/149‑1 (MQ=255) aaaaCGGCACCAGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTAc < 3:278053/112‑1 (MQ=255) aaaaCGGCACCAGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTAc > 4:278053/1‑112 (MQ=255) gccccTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCaga < 4:773149/95‑1 (MQ=255) gCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCaga > 3:773149/1‑98 (MQ=255) cACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGGCGCGGGCCACAt > 1:259979/1‑149 (MQ=255) aCCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGGCGCGGGCCACAtg > 3:1353529/1‑149 (MQ=255) cTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGGCGCGGGCCACAtgtg > 1:1042889/1‑149 (MQ=255) | GCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGT‑AAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGGCGCGGGCCACATGTG > NC_000913/1448911‑1449197 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |