Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F69 I0 R1	1426	148.0	5875178	97.4%	5722423	123.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,230,415	T→A	8.6%	intergenic (‑30/‑209)	dusC ← / → yohJ	tRNA‑dihydrouridine synthase C/UPF0299 family inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,230,415	0	T	A	8.6%	274.1 / 12.6	81	intergenic (‑30/‑209)	dusC/yohJ	tRNA‑dihydrouridine synthase C/UPF0299 family inner membrane protein
Reads supporting (aligned to +/- strand): ref base T (35/39); new base A (2/5); total (37/44)
Fisher's exact test for biased strand distribution p-value = 4.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

CCACCACGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGC  >  NC_000913/2230274‑2230547

ccaccaCGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAgcg                                                                                                                               <  3:807350/149‑1 (MQ=255)

 caccacGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAgcgc                                                                                                                              <  1:443007/149‑1 (MQ=255)

   ccacGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTAACATAGCGCCt                                                                                                                            <  1:1075753/149‑1 (MQ=255)

         ggACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTc                                                                                                                      <  2:441186/149‑1 (MQ=255)

            cAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGc                                                                                                                   <  4:102121/149‑1 (MQ=255)

               aCTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGa                                                                                                                <  3:689462/149‑1 (MQ=255)

                     tGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTAACATAGCGcc                                                                                                                             <  2:308862/130‑1 (MQ=255)

                       aTGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTa                                                                                                                                  <  4:1308152/123‑1 (MQ=255)

                       aTGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTa                                                                                                                                  >  3:1308152/1‑123 (MQ=255)

                         gCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCt                                                                                                      >  2:1229170/1‑149 (MQ=255)

                           acaGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGtt                                                                                                                                   <  4:947769/118‑1 (MQ=255)

                           acaGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGtt                                                                                                                                   >  3:947769/1‑118 (MQ=255)

                           acaGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGc                                                                                                    >  3:1142668/1‑149 (MQ=255)

                           acaGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCACCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGc                                                                                                    >  1:726605/1‑149 (MQ=255)

                             aGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCaa                                                                                                  >  1:1168912/1‑149 (MQ=255)

                               aTCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGAt                                                                                                               >  2:1059426/1‑134 (MQ=255)

                               aTCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGAt                                                                                                               <  1:1059426/134‑1 (MQ=255)

                                     gTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGAt                                                                                          <  2:448644/149‑1 (MQ=255)

                                        gTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATtgc                                                                                       >  1:1054525/1‑149 (MQ=255)

                                        gTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATtgc                                                                                       >  1:599692/1‑149 (MQ=255)

                                        gTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATtgc                                                                                       >  3:220845/1‑149 (MQ=255)

                                         ttAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATtgct                                                                                      <  1:981511/149‑1 (MQ=255)

                                          tAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATtgctg                                                                                     <  2:1168912/149‑1 (MQ=255)

                                                 ggTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTg                                                                              <  2:1054525/149‑1 (MQ=255)

                                                   tCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATtg                                                                                        <  4:1046064/137‑1 (MQ=255)

                                                   tCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATtg                                                                                        >  3:1046064/1‑137 (MQ=255)

                                                   tCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCa                                                                            >  2:1142148/1‑149 (MQ=255)

                                                    cAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCaa                                                                           <  4:1142668/149‑1 (MQ=255)

                                                       cAATTCACGCACCAGAGAGTCAAGCACTCCCACCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATcc                                                                        <  2:726605/149‑1 (MQ=255)

                                                         aTTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTaaa                                                                                                           <  4:568239/112‑1 (MQ=255)

                                                         aTTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTaaa                                                                                                           >  3:568239/1‑112 (MQ=255)

                                                         aTTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTAACATAGCGCCtc                                                                                                                           <  1:1060501/96‑2 (MQ=255)

                                                          ttCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTa                                                                                                                                  >  2:584695/1‑88 (MQ=255)

                                                          ttCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTa                                                                                                                                  <  1:584695/88‑1 (MQ=255)

                                                             aCGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAag                                                                                                >  1:544167/1‑119 (MQ=255)

                                                             aCGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAag                                                                                                <  2:544167/119‑1 (MQ=255)

                                                                 aCCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCaaa                                                                                                 <  1:883652/114‑1 (MQ=255)

                                                                 aCCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCaaa                                                                                                 >  2:883652/1‑114 (MQ=255)

                                                                     gagagTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTg                                                                              <  2:1207253/129‑1 (MQ=255)

                                                                     gagagTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTg                                                                              >  1:1207253/1‑129 (MQ=255)

                                                                            aaGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCaaa                                                                                 >  4:233168/1‑119 (MQ=255)

                                                                            aaGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCaaa                                                                                 <  3:233168/119‑1 (MQ=255)

                                                                             aGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAagag                                                                                              <  3:1006347/105‑1 (MQ=255)

                                                                             aGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGcc                                                  <  1:1142148/149‑1 (MQ=255)

                                                                             aGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATCAAAGGTCTGCAAagag                                                                                              >  4:1006347/1‑105 (MQ=255)

                                                                                 ctccctccATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGAt                                                                                                               <  4:681186/84‑1 (MQ=255)

                                                                                 ctccctccATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGAt                                                                                                               >  3:681186/1‑84 (MQ=255)

                                                                                  tccctccATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTc                                                                                                       <  3:693509/91‑1 (MQ=255)

                                                                                  tccctccATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTc                                                                                                       >  4:693509/1‑91 (MQ=255)

                                                                                  tccctccATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGt                                             >  4:1416486/1‑149 (MQ=255)

                                                                                   ccctccATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCaaa                                                                                 >  3:775681/1‑112 (MQ=255)

                                                                                   ccctccATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCaaa                                                                                 <  4:775681/112‑1 (MQ=255)

                                                                                      tccATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGa                                                                                           >  2:82177/1‑99 (MQ=255)

                                                                                      tccATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGa                                                                                           <  1:82177/99‑1 (MQ=255)

                                                                                      tccATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGc                                         <  3:1416486/149‑1 (MQ=255)

                                                                                         aTCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATg                                                                                                                         >  2:449379/1‑66 (MQ=255)

                                                                                         aTCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATg                                                                                                                         <  1:449379/66‑1 (MQ=255)

                                                                                          tCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATcc                                                                        >  4:221632/1‑114 (MQ=255)

                                                                                          tCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATcc                                                                        <  3:221632/114‑1 (MQ=255)

                                                                                                 cAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAAt                                                                          >  2:254968/1‑105 (MQ=255)

                                                                                                 cAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAAt                                                                          <  1:254968/105‑1 (MQ=255)

                                                                                                      acacacGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCt                                                 >  1:1428770/1‑125 (MQ=255)

                                                                                                      acacacGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCt                                                 <  2:1428770/125‑1 (MQ=255)

                                                                                                        acacGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGa                                               >  2:232577/1‑125 (MQ=255)

                                                                                                        acacGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGa                                               <  1:232577/125‑1 (MQ=255)

                                                                                                          acGCATATCATCACCCGCAAAAAAATGAGGCGCTGTGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCt                                        <  3:446537/130‑1 (MQ=255)

                                                                                                          acGCATATCATCACCCGCAAAAAAATGAGGCGCTGTGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCt                                        >  4:446537/1‑130 (MQ=255)

                                                                                                          acGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAg                                                                                                          <  3:114142/64‑1 (MQ=255)

                                                                                                          acGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAg                                                                                                          >  4:114142/1‑64 (MQ=255)

                                                                                                               tatCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGAt                                                         <  2:415024/108‑1 (MQ=255)

                                                                                                               tatCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGAt                                                         >  1:415024/1‑108 (MQ=255)

                                                                                                                  catcaCCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGcc                       <  1:1309993/139‑1 (MQ=255)

                                                                                                                  catcaCCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGcc                       >  2:1309993/1‑139 (MQ=255)

                                                                                                                              aaaaaTGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCt                                                                                                      >  1:1019966/1‑48 (MQ=255)

                                                                                                                              aaaaaTGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCt                                                                                                      <  2:1019966/48‑1 (MQ=255)

                                                                                                                                    taGGCGCTAACATAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAgcgc  >  2:331054/2‑142 (MQ=255)

                                                                                                                                    taGGCGCTAACATAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAgcgc  <  1:331054/141‑1 (MQ=255)

                                                                                                                                      ggCGCTAACATAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCggg                    <  2:896005/122‑1 (MQ=255)

                                                                                                                                      ggCGCTAACATAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCggg                    >  1:896005/1‑122 (MQ=255)

                                                                                                                                         gcTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCAtt                                 >  2:199640/1‑106 (MQ=255)

                                                                                                                                         gcTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCAtt                                 <  1:199640/106‑1 (MQ=255)

CCACCACGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGC  >  NC_000913/2230274‑2230547

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A